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Lab Report 2

The document describes an experiment measuring the reaction time of yeast spheres in hydrogen peroxide solutions of different concentrations. It was hypothesized that increasing the hydrogen peroxide concentration would speed up or slow down the reaction. Data was collected by timing how long it took yeast spheres to rise from bottom to top in 0.12%, 0.3%, and 0.6% hydrogen peroxide solutions. The results showed reaction times decreased with increasing concentration, supporting the hypothesis. Variations in yeast sphere size were identified as a potential source of error.

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86 views3 pages

Lab Report 2

The document describes an experiment measuring the reaction time of yeast spheres in hydrogen peroxide solutions of different concentrations. It was hypothesized that increasing the hydrogen peroxide concentration would speed up or slow down the reaction. Data was collected by timing how long it took yeast spheres to rise from bottom to top in 0.12%, 0.3%, and 0.6% hydrogen peroxide solutions. The results showed reaction times decreased with increasing concentration, supporting the hypothesis. Variations in yeast sphere size were identified as a potential source of error.

Uploaded by

katelyn
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Page 1

Katelyn Dalton 10/1

Title: Measuring Catalyst Activity

Purpose: Measuring catalyst activities with different substrate


to see if the enzyme will still ‘fit’ in the active site.

Hypothesis: When the volume of hydrogen peroxide is champed the


reaction will either delay or happen quickly.

Procedure
- mixture of equal parts 10% yeast solution and 2% sodium alginate,
dropped in a cup with 50 Ml of 0.15M of CaC13 creating spheres of
yeast
- A yeast sphere is dropped into a solution with 0.12% of Hydrogen
peroxid
- Time from when the sphere hits the bottom to when it reaches the top
- Repeat 10 times
- A yeast sphere is dropped into a solution with 0.3% of Hydrogen
peroxid
- Time from when the sphere hits the bottom to when it reaches the top
- Repeat 10 time
- A yeast sphere is dropped into a solution with 0.6% of Hydrogen
peroxid
- Time from when the sphere hits the bottom to when it reaches the top
- Repeat 10 times

Page 2

Data Collection

0.12 0.3 0.6

1 20.34 6.7 4.0

2 19.04 6.88 2.73

3 17.73 6.81 3.75

4 17.62 6.65 2.98

5 18.58 6.42 4.04

6 18.64 5.86 3.99

7 17.11 6.54 3.76

8 20.43 7.24 3.56

9 18.24 6.19 2.78

10 18.33 6.82 4.28

AVG 18.6 6.1 3.59

Sources of Error
The main source of ever inferred is the size of he yeast spheres
made, without the spheres being the same size for each drop in the
solutions the most accurate average can’t be concurred. As well as
stopping the timer used on the phone there could be a couple
milliseconds that are counted on accident with how fast the timer is
stopped.

Page 3

Summary:

In correlation to the hypothesis with the reaction was different

depending on the volume of hydrogen peroxide in the test tubes the

data table shows a correspondence to it, when the higher the volume of

the hydrogen peroxide was added the In the solution the faster the

yeast spheres went top the top of the the test tube. In each solution

there an outlier, in 0.12% the outlier was 17.11 seconds. In 0.3% the

outlier was 7.27 seconds, and in 0.6% the outlier was 4.28 seconds.

Which could correspond to the sources of error with different masses

of Yeats spheres dropped in the solutions.

In conclusion the lab showed the reaction of enzymes in a

specific solutions and how with different variants of specific

mixtures can cause the reaction time to either delay and or react

quicker depending on the amount added

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