For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 7
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2. Date Submitted:
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Expt. 7 Salivary Digestion
LAB REPORT Member 1 Member 2 Member 3 Member 4 Member 5
I. SCORE
Pre-lab Data
Data and Observations
Post-lab Questions
Sub-total
Participation
Online Pre-lab Quiz
Total Score
Objectives
II. Apparatus: III. Symbols of Chemicals:
IV. Procedure: (Note: Write schematic diagram on the sheet provided.)
V. Data and Observations
QUALITATIVE TEST OBSERVATION
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For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 7
Microscopial Examination Sketch of the unfiltered saliva viewed thru a microscope
unfiltered saliva stained with
methylene blue and viewed thru the
microscope
Salivary microscope constituents
pH Determination Estimated pH of saliva:
Test for mucin Identity of the precipitate formed?
3 mL saliva + 1-2 drops dil. HC2H3O2
What is the function of this precipitate?
Test for protein (Biuret test)
Test for Inorganic Matter
5 mL of saliva + 1 drop of 6N HNO3,
heated to boiling and filtered.
a) Test for chlorides Color:
filtrate + AgNO3 Name of ppt.:
Equation:
b) Test for phosphates Color:
filtrate + (NH4)2MoO4 Name of ppt.:
Equation:
c) Test for sulfates Color:
filtrate + BaCl2 Name of ppt.:
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Equation:
d) Test for calcium Color:
filtrate + (NH4)2C2O4 Name of ppt.:
Equation:
Test for thiocyanate (FeCl3 test)
a) saliva + dil. FeCl3 + dil. HCl till acidic Color:
b) + 1 drop HgCl2 Color
c) saliva + distilled water Color
comparing results from a) and c)
Source of thiocyanate in saliva
DIGESTION OF STARCH PASTE
TIME COLOR of I2 + SALIVA
1st minute
2nd minute
3rd minute
4th minute
5th minute
6th minute
DIGESTION OF STARCH PASTE
CONDITION OBSERVATION
Fehling’s Test at achromatic point Color:
What is the reduction due to?
Time it took for complete transformation of
starch to sugar
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For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 7
SEPARATION OF THE PRODUCTS OF SALIVARY DIGESTION
CONDITION OBSERVATION
25 mL of 1% starch paste + 1 mL saliva, stirred, Color:
+ iodine, +50 mL of 95% alcohol, allowed to stand
undisturbed.
Residue
a) Benedict’s test
b) Dextrin ppt. subjected to iodine test.
INFLUENCE OF FREE ACID
CONDITION OBSERVATION
Dark Blue Solution
tt #1 4 mL 0.2% HCl + 1 mL starch paste + 1 drop saliva
(acidity = 0.16%)
Dark Blue Solution
tt #2 4 mL 0.5% HCl + 1 mL starch paste + 1 drop saliva
(acidity = 0.04%)
Dark Blue Solution
tt #3 4 mL 0.0125% HCl + 1 mL starch paste + 1 drop saliva
(acidity = 0.01%)
Dark Blue Solution
tt #4 4 mL 0.006% HCl + 1 mL starch paste + 1 drop saliva
(acidity = 0.0048%)
INFLUENCE OF ALKALI
CONDITION OBSERVATION
Dark blue ppt
tt #1 4 mL 2% Na2CO3 + 1 mL starch paste + 1 drop saliva
Deep blue solution
tt #2 4 mL 1% Na2CO3 + 1 mL starch paste + 1 drop saliva
tt #3 4 mL 0.5% Na2CO3 + 1 mL starch paste + 1 drop saliva Deep burgundy color (red/black)
suspension
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For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 7
Brick red ppt
tt #4 4 mL 0.05% Na2CO3 + 1 mL starch paste + 1 drop saliva
POST-LAB QUESTIONS
1. What is the role of mucin in the saliva? (6 pts)
2. Give 3 constituents of saliva and identify the functions of each? (18 pts)
a.
b.
c.
3. Salivary amylase is present in the mouth and its isoenzyme is present in the intestine, however,
it is not present in the stomach. Why? (10 pts)
The stomach produces its own amylase, which is called gastric amylase. Only the amylase produced by the
stomach, among the amylases produced by the saliva and pancreas, is active in the stomach. Gastric
amalyse is special that it can function in an acidic environment without itself being deformed.
Furthermore, the pH in the stomach is around 1.0 to 2.0, which is extremely acidic. This level of acidity
causes the salivary amylase's protein structure to denature and change shape. With that, salivary amylase
is inactivated by an acid pH, no significant hydrolysis of carbohydrates would then occur in the stomach.
Consequently, salivary amylase does not function once it enters the stomach (West, n.d.).
THIS MATERIAL IS OWNED BY THE CHEMISTRY DEPT OF XAVIER-ATENEO 5
For the exclusive use of Chem 153L class in Xavier-Ateneo EXPERIMENT 7
West, J. (n.d.). Enzymes That Play an Important Role in Food Digestion. Seattle Post-Intelligencer.
https://education.seattlepi.com/enzymes-play-important-role-food-digestion-4062.html
4. What is an Achromatic point and what does this suggest? (6 pts)
Schematic Diagram of Procedure
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