Centrifugal Filters

Guide to Converting g-Force to RPM

1. Measure the Radius of the Rotor
Obtain the radius (r) of the rotor from the 25,000 2. Use Nomogram to Calculate RPM
manufacturer’s specifications or measure Plot the radius of the rotor and the recommended RCF (g-force) of the
the radius as shown below. 20,000 device on the nomogram and then draw the line to determine RPM. 500
The nomogram is based on the formula below. 475
15,000 450
Fixed Angle RCF
= RPM
425
(1.118 x 10 -6) (Radius in mm) 400
375
10,000 350
30,000 Amicon® Ultra Centrifugals
14,000 xg = Amicon® 0.5 mL (FA, concentration) 325
20,000
7,500 xg = Amicon® 2 mL (FA), A
 micon® 4 mL 300
(FA, 3,10,30,50k)
6,000 10,000 5,400 xg = Amicon® 2 mL (FA, dense solutions*) 275
5,000 xg = Amicon® 4 ml (FA, 100k),
250
Amicon® 15 mL (FA)
5,000 4,000 xg = Amicon® 2 mL, 4 mL, 15 mL (SB)
4,000 225
1,000 xg = A micon® 0.5 mL (reverse spin), 
Amicon® 2mL (reverse spin)
Swinging Bucket 3,000 2,000
200
Specialized Centrifugals
Swinging bucket 14,000 xg = Microcon® Protein (FA)
175
running position 1,000 12,000 xg = Ultrafree® -MC (FA)
2,000 5,000 xg = Ultrafree® -CL (FA)
3,500 xg = Centricon® Plus-70 (SB) 150
500 1000 xg = Microcon® (reverse spin)
500 xg = Microcon® DNA (FA)
140
130
200 120
1,000 110
100
100
50
90

500 80
20
70
10 FA = fixed angle
SB = swinging bucket
*For example, undiluted serum, plasma. 60
Use our online Amicon® selector
tool to choose the perfect filter 3
and view protocols, visit: 200 50
www.emdmillipore.com/FastEasy RCF (g) Radius of
RPM Rotation (mm)

When downstream results matter, make sure your upstream tools are the best.
Concentrate and fractionate your samples without compromising yield or function using EMD Millipore’s fast, gentle, membrane-based solutions.

Small Volume Medium Volume Large Volume XL Volume

<1 mL <2 mL <4 mL <15 mL <70 mL

Microcon® DNA/ Ultrafree®-MC Amicon® Ultra – 0.5 Ultrafree®-CL Amicon® Ultra–2 Amicon® Ultra–4 Amicon® Ultra–15 Centricon® Plus – 70
Microcon® Centrifugal Filter Centrifugal Filter Centrifugal Filter Centrifugal Filter Centrifugal Filter Centrifugal Filter Centrifugal Filter
Centrifugal Filter

To place an order or for complete product information,

EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany visit: www.emdmillipore.com/psp
EMD Millipore, the M mark, Amicon, Centricon, Microcon and Ultrafree are registered trademarks of Merck KGaA, Darmstadt, Germany.
Lit. No. PS4547EN00 Rev A BS-15-11378 05/2015 ©2015 EMD Millipore Corporation, Billerica, MA, USA. All rights reserved.