VWRTM VistaVisionTM

User Guide

Metallurgical Microscope

Model

82026-636

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 Congratulations and Thank You for your purchase of a

VWR VistaVision Microscope

This precision instrument has been designed to require a minimum of optical
and mechanical maintenance. Its excellent design assures years of high quality,
reliable service. We recommend that you read this entire manual carefully
before beginning to use the instrument.

WARNING
PLEASE DISCONNECT PLUG FROM THE MAIN SOCKET BEFORE
REMOVING THE BOTTOM PLATE, OPENING THE LAMP
REPLACEMENT DOOR, OR REMOVING THE LAMP HOUSE.

WARNING
THE POWER CORD PROVIDED WITH THE EQUIPMENT HAS A
GROUNDED PLUG. ALWAYS USE THE POWER CORD WITH A
PROPERLY GROUNDED WALL OUTLET.

DO NOT EXPOSE THE INSTRUMENT TO HIGH TEMPERATURE OR

HUMIDITY. AVOID USING THE INSTRUMENT IN EXTREMELY DUSTY
CONDITIONS
OPERATING TEMPERATURE: 5 DEGREE C. TO 35 DEGREE C.
OPERATING HUMIDITY: 20% TO 80% @ 25 DEGREE C.

CAUTION: NEVER IMMERSE THE INSTRUMENT IN WATER OR

SOLVENT

CAUTION: DO NOT PUT ANY FOREIGN OBJECTS IN THE FRAME OR

INTO ANY MOVING MECHANICAL PARTS
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 Table of Contents

List of Contents................................................................................... 3
Getting Started .................................................................................... 3
Components ........................................................................................ 4
Technical Specifications ..................................................................... 5
Setup Instructions ............................................................................... 6
Basic Operation .................................................................................. 7
Changing the Incident (upper) Light Bulb........................................ 13
Changing the Transmitted (lower) Light Bulb.................................. 13
Preventative Maintenance ................................................................. 14

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 List of Contents
Frame
Optical Body with Vertical Illumination Tube
10X Eyepieces (2)
5X Plan Achromatic Objective
10X Plan Achromatic Objective
20X LWD Plan Achromatic Objective
40X LWD Plan Achromatic Objective
50X LWD Plan Achromatic Objective
Vertical Illumination Lamp House
Analyzer Filter
Two Filters: Blue and Frosted
Two Spare Halogen Bulbs
Spare Fuse
Power Cord
Dustcover
Manual

Getting Started
This reference manual is written on the assumption that it will be used by
advanced students and experienced microscopists. It covers the essential
adjustments and controls of VWR VistaVision microscopes. No attempt has
been made to include fundamentals of microscopy. Read this user guide prior
to unpacking the microscope. After assembling the instrument, re-read the
manual and become familiar with all adjustments and functions before using
your new microscope.

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 Components
1 2 3 4 5 6 7 8 9 10

11

12

13

14
27

26

25

24

23

22 21 20 19 18 17 16 15

Figure 1
1. Eyepiece (2) 2. Trinocular Body 3. Polarizer 4. Field Iris Diaphragm 5. Field Iris
Diaphragm Centering Screws 6. Filter Wheel 7. Aperture Iris Diaphragm 8. Screw of
Lamp House 9. Focusing Lens Lever 10. Lamp House 11. Lamp Adjustment Lever
12. Locking Screw 13. Coarse Focusing Knob 14. Fine Focusing Knob 15. Plug for
Vertical Illumination 16. Transmitted / Incident Illumination Toggle Switch 17.
Lengthwise Knob 18. Cross Knob 19. Collector 20. Field Diaphragm 21. Abbe Condenser
and Iris Diaphragm with Filter Tray 22. Condenser Centering Screws 23. Abbe Condenser
Locking Screw 24. Stage 25Objectives 26. Tube
4 Holding Screw 27. Analyzer Lever

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 Technical Specifications
Body: Optical Body, inclined 30°, able to rotate a full 360°with
53mm to 75mm interpupillary adjustment. Left Eye diopter
adjustment of -5 to +5.
Nosepiece: Quintuple ball-beating reversed nosepiece with positive click
stops and smooth operation.
Eyepieces: 10X Plan wide field, Focal Length 25mm, Field Φ18mm.
Stage: Integrated Mechanical Stage size 185mm X 140mm with Glass
insert 98mm X 77mm. Right–hand Coaxial drop–down X–Y
Control Knobs. Range of motion 75mm X 50mm.
Focus Mechanism: All stainless steel and brass gear train system
Adjustable Tension Control to prohibit drift and Adjustable
Up-Stop to protect slides, specimen and objective lenses.
Coaxial coarse and fine adjustments with dial markings at
0.002mm increments.
Incident Light System: Condenser, Field and Aperture Iris diaphragm,
four color Filter Wheel and Polarizing filter
Transmitted Light System: 1.25 N.A., Abbe Condenser with iris diaphragm
Rack and Pinion Focusing, Easily centered with two adjustment
knobs.
Illumination: 20 watt, 6 volt Halogen bulb with electronic dimmer for
Incident and Transmitted light systems. Fuse: 0.5 Amp, located
in the base

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 Objective specifications: R indicates retractable lens
Field of
DIN Standard Magnificat Numerical Working
View with
Objectives ion Aperture Distance
10X E.P.
Plan achromatic 5X 0.12 18.3mm 3.6mm
Plan achromatic 10X 0.25 8.9mm 1.8mm
LWD planachro. 20X R 0.40 4.8mm 0.9mm
LWD planachro. 40X R 0.60 3.73mm 0.45mm
LWD planachro 50X R 0.70 2.02mm 0.36mm

Setup Instructions
1. Remove all parts from their packing materials and retain the packaging in
the event you need to transport the product.
2. Loosen the thumbscrew and install the Optical Body with Vertical
Illumination Tube to the frame by removing its dust cap and inserting the
dovetailed flange on the underside of the optical body into the frame.
Secure optical body with thumbscrew. DO NOT RELEASE THE
OPTICAL BODY FROM YOUR GRASP UNTIL IT IS FIRMLY
SECURED TO THE FRAME.
3. Install the objective into the nosepiece.
4. Remove the dust caps from the eyetubes in the Optical Body and insert
both eyepieces into the Optical Body.
5. Install the lamp house.
6. Connect the power cord to the instrument and to a suitable power supply.

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 Basic Operation
1. Illumination controls
a) The power switch to the illuminator and
the brightness control are located on
the base. The transmitted / incident
illumination toggle switch 1(Figure 2B)
is located on the right side. The 1 2
A
electrical system is fuse protected and 1. Power switch 2. Brightness
control
the fuse holder, located in the base of
the microscope.
b) Turn on the light with the Power switch
1 (Figure 2A), see Figure 2. If the light
does not appear to be ON, check the
brightness control 2( Figure 1A) to see 1
B
if it’s on a sufficiently low setting.
1. Transmitted / Incident
Also check the transmitted / incident illumination toggle switch
switch is set on the transmitted setting. Figure 2
2. Focusing Controls
1 2 3 4
a) Focusing adjustment is accomplished
by using the large coarse adjustment
knobs located comfortably on each side
of the frame. Fine adjustment is
accomplished using the smaller knobs
located on the same focus shaft. This
coaxial arrangement allows for easy,
Figure 3
precise adjustment without drift or
1. Fine Focus 2. Focus
discomfort.
Tension 3. Up Stop 4.
b) Focus Control Turning either of the
Coarse Focus
coarse focus control knobs will raise or
lower the stage. The smallest graduation on the fine adjust knob index
scale is 2μm of vertical.

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 c) Focus Tension Adjustment The tension of the coarse focus is
adjustable and preset at the factory for ease of use. If you wish to
adjust the coarse focus tension, first locate the tension adjustment ring
on the left coarse adjustment knob. It is located between the frame and
right coarse adjustment knob. Turning the ring toward the rear of the
microscope increases the tension, and toward the front of the
microscope loosens it. Tension is too high if you experience physical
discomfort
d) Pre-focusing or Focus Stop Control This lever is located on the right
side of the frame, between the coarse focus knob and the frame. Use of
this feature will insure that the longer objectives don’t contact the stage
or specimen when using the microscope. Its use also simplifies
focusing. After focusing on the specimen with the coarse adjustment,
rotation of the lever toward the front of the microscope will set the
upper limit on the coarse adjustment movement. After changing
specimens or objectives, focusing is easily accomplished by rotating
the coarse adjustment knob to reach the pre-focused position, then
making fine adjustments with the fine adjustment knob. Focusing
movement with the fine adjustment knob isn’t affected by using the
pre-focusing lever
3. Interpupillary and Diopter Adjustments
a) Diopter Adjustment Proper
correction for individual vision is
accomplished via the diopter
adjustment located at the left eyepiece,
see Figure 4. Using the 40X objective,
close your left eye and bring and
image into focus with your right eye
only. Once the image is well focused,
close your right eye and check the
focus with your left eye. If the image ①4
Figure
is out of focus, make fine adjustments
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 with the diopter adjustment ring to correct for your vision.
b) Proper interpupillary distance, or the distance between eyepieces, is
crucial to the comfort of the user. Adjusting the interpupillary distance
is accomplished through a “folding” action of the Seidentopf design
optical head, see Figure 4, allowing for quick and easy adjustment.
4. Mechanical Stage Controls
The mechanical stage, see Figure 5, with
Ultra-Low position control knobs for ease of use,
consists of three parts:
Y-Axis
a) The smaller knob for X-Axis or transverse
adjustment. X-Axi
Figure 5
b) The larger knob for Y-Axis or longitudinal
adjustment.
5. Condenser Components – are located
below the stage as follows, see Figure 6: 1
Abbe condenser; its Mounting Ring with
lock screw and two large centering screws
2
at either side for adjusting condenser/iris
3
assembly in the optical center of the light
path; the adjustment knob for raising and
lowering the condenser lens. The substage
lamp house includes a field iris diaphragm 4 5 6 7
Figure 6
for Kohler illumination and a recess for 1. Stage 2. Abbe Condenser
installation of a filter, if desired. Centering Screws 3. Abbe
Condenser Lock Screw 4. Iris
6. Condenser Alignment Adjustments to the Diaphragm 5. Abbe Condenser
substage condensing system, see Figure 7, 6. Substage Lamp House with
Field Iris Diaphragm 7. Filter
are crucial for proper illumination and Holding Tray
performance. There are three basic
adjustments to be made: centering, vertical focusing and aperture
adjustment.

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 a) Centering–The condenser must be
centered in the light path to ensure
proper light control. One simple
method for centering follows:
i) Rotate the objective nosepiece until
the 5X objective is in the light path.
Substage
ii) While looking into the microscope, Adjustment Knob
close the field diaphragm to the Figure 7
smallest setting using the knurled ring
at the top of the light exit window
assembly.
iii) Closing the iris in this manner will
A B C
reduce the field so that a small white
circle is visible within a black field, see Figure 8
Figure 8A. This white circle is the light
that is passing through the iris and it should be centered in the black field
as in Figure 8b. If it is not centered, move it to the center using the
centering screws.
iv) By opening the iris until the white circle almost fills the entire field,
see Figure 8C.
b) Vertical Focusing–The condenser can be raised and lowered with the
sub-stage adjustment knob in order to focus the light for optical
illumination.
c) Aperture Adjustment–The contrast can be adjusted with the iris
diaphragm adjustment lever on the condenser assembly. Aperture
adjustments are made to induce contrast into a specimen, not to adjust
light intensity.

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 7. Vertical Illumination Alignment, See Figure 9.
1 2 3 4 5 6

11 10 9 8 7

Figure 9
1. Trinocular Body 2. Field Iris Diaphragm 3. Filter Wheel 4. Aperture Iris
Diaphragm 5. Lamp Adjustment Lever 6. Locking Screw 7. Focusing Lens Lever 8.
Screw of Lamp House 9. Field Iris Diaphragm Centering Screws 10. Polarizer
11 A l l
1) To center the incident or vertical lamp put a small piece of paper and
place it on the stage. Take out one objective from the nosepiece and
rotate the nosepiece until this objective opening hole is in position
over the paper.
2) Turn the incident (upper) illumination on and adjust the lamp
intensity control to establish sufficient light.
3) This paper will allow for focusing of the lamp filament on the paper.
Refer to Figure 2 for this step. Reduce the aperture iris diaphragm,
using the adjustment lever and rotate the lamp focusing control on
the lamp housing until a sharp image of the lamp filament is attained.
4) Refer to Figure 6 for this step. Filament
If the filament image is not
centered in the overpass hole,
loosen the lamp set screw and
move the bulb socket with the
lamp centering knob to obtain a
Overpass Hole
uniform image. Retighten the set Figure 6
screw.
11

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 5) Reopen the aperture iris diaphragm.
6) Reinstall the objective again.
7) The Iris diaphragm nearest the illuminator house in the vertical
illuminator tube may be closed slightly to enhance contrast. The iris
diaphragm nearest the illuminator house should be centered for
Kohler illumination.
8) There is a field diaphragm near the trinocular body for Kohler
Illumination.
9) The Filter wheel contains a green, yellow, blue, and neutral density
filters, as well as a clear opening.
10) The Polarizing Analyzer is used in
1
conjunction with the Polarized
filter cube, selected by pushing in
the blue filter rod (Figure 10). The
Analyzer may also be placed over
the lower light source on the base 2
(Figure 7) and used with the
Polarized filter cube for 3 4
transmitted light work. Figure 10
1. Camera Port 2. Push Rod to Direct
11) The white push rod to the upper
Image to Binocular Eyepieces or
left of the trinocular head will Camera Port (White) 3.Silver Rod for
send 100% of the image light to Normal Upper Light 4. Blue Rod for
the binocular eyepieces or to the Analyzer Filter
top port.

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 Changing the Incident (upper) Light Bulb
1. Turn off and unplug the microscope.
2. Loosen the lamp set screw, Figure 11, unscrew the lamp centering control
lever and remove the entire lamp 1
socket from the lamp house. 2
3
3. The halogen bulb can be
4
removed by grasping the bulb
and pulling it firmly from its
fixture. Take care not to touch
the lamp with bare fingers, as
the lamp will be hot. Insert the Figure 11
1. Lamp Adjustment Lever 2. Lamp Set Screw
new halogen bulb into the same
3. Bulb 4. Lamp Socket
fixture. When installing the new
bulb, be careful not to touch the
glass with your fingers. The new lamp should be supplied in a plastic
protective envelope. If not, use a tissue or other medium to grasp the bulb.
This will prevent contamination from your hand from reducing the bulb’s
intensity and life.
4. Reinsert the lamp socket into the lamp house and retighten the setscrew.
Make certain that the new lamp is placed squarely in the socket to insure
that is will center properly in the field of view. You may need to re–center
the lamp as previously described.

Changing the Transmitted (lower) Light Bulb

Disconnect the power plug and wait to be sure the bulb is cool. Locate the
hinged door on the bottom of the frame. The lamp door retaining screw is on
the front of the frame, and the lamp centering screw is located in the middle
of the lamp door. Open the lamp door using the retaining screw as shown in
Figure 12. Pull the old lamp straight out of the socket. Always use a clean
cloth or lens tissue to prevent grease or oil getting on the glass of the new

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 bulb. Insert the lamp pins straight into the
socket. Close the lamp door and center
the lamp in the field of view using the
lamp positioning screw at the center of
the lamp door and pulling it firmly, see
Figure 12
1 3
2

Figure 12
1. Bulb 2. Lamp Door Retaining
Screw 3. Lamp Centering Screw
Preventative Maintenance
1. Cleaning frame and stage
Disconnect the plug from main socket before cleaning. Clean the frame
and stage with a soft cloth moistened with a mild detergent solution. Be
sure the instrument is dry before using.
2. Cleaning optical parts
Microscope eyepieces and objectives are coated.
They should not be wiped while dry as dirt or dust may scratch the
coating. It is best to remove parts from the frame prior to cleaning.
Always blow loose dust away first. Use cotton swabs or lens tissue
moistened with a lens cleaner or a small amount of alcohol, then wipe the
surface clean with a good quality lens tissue. Solvents such as Xylene
should NOT be used as cleaner.

DO NOT DISASSEMBLE OBJECTIVE LENSES.

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 VWR TECHNICAL HOT LINE: 888-VWR-LINE

(888-897-5463)

15

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www.vwr.com

VWR Compound Microscope

Operation Manual
(Version 1.0.0)

VWR Compound Microscope

89404-468, 89404-470
89404-472, 89404-474

89404-468 VWR Microscope Compound Microscope Trino Plan

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SAFETY MANUALS

Please read this manual carefully before using this product for optimal use. The indicated cautions are
related to safety and you should observe all safety and warning instructions to avoid potential damage
to product and injury to operators. Keep this manual for future reference.

Note: Use this product only in a way described in the product literature and this manual. Before using
the product, verify that this product is suitable for its intended use.

Do not modify the system components or use the unauthorized parts as this will void the product
warranty.

The following warning labels (or symbols) are found on the microscope, Study the meaning of the
warning labels (or symbols) and always use the equipment in the safest possible manner.

Warning Label / Symbol Explanation

Indicates that the surface becomes hot, and
should not be touched with bare hands.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

~ Indicates alternating current.

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INTRODUCTION

Thank you for your purchase of a VWR microscope. VWR microscopes are precision instruments, subjected
to meticulous examination in order to reach you in perfect condition. Their design combines easy
management and optimum functioning with minimum maintenance.
The information contained in this manual is likely to go beyond what the average user needs to know to use
the microscope, however, it is provided to answer any queries that may arise.

Your new microscope combines high performance features, with an excellent degree of optical resolution
and clarity of image. It incorporates a mechanical stage that provides a travel range of 70mm x 50mm in X
and Y directions with a graduation of up to 0.1 mm, thus permitting the perfect positioning of the specimen.
Also included are objectives located on a ball bearing nosepiece allowing movement in both directions; a
precision coarse and fine focusing system; a moveable Abbe condenser with a numerical aperture of 1.25
N.A. and a built-in 12V/ 20W halogen variable light source.

These instructions should be read carefully before operating the microscope. They will permit you to use
your new microscope to its fullest capabilities. Terminology used to describe components and controls can
be found in the diagram on page 2.

These instructions are based on the assembly and use of the binocular model with additional notes
applying specifically to other models in the series.

UNPACKING

All components of the microscope have been carefully packed to make sure they reach you in perfect
condition. We recommend that you do not discard any packing containers in case you need to return the
microscope or store it for long periods of time; or should it become necessary to transport it to a technical
service for any repair, or maintenance procedure.

The box should contain the following components, depending on the model:

 Binocular model: Stand, Power cable, monocular head, two eyepieces, two eyepiece protectors, four
objectives, condenser, sample holder mechanism, blue, green and yellow and frosted filters, fuse, dust
cover, a bottle of immersion oil and a 2mm hexagonal key.

 Trinocular model : Stand, Power cable, trinocular head, two eyepieces, two eyepiece protectors, four
objectives, condenser, sample holder mechanism, blue, green and yellow and frosted filters, fuse, dust
cover, a bottle of immersion oil and a 2mm hexagonal key.

Remove, and handle the microscope and all its components with extreme care.

Avoid touching the lenses of the optical elements and keep clear of contact with dust, water or other
contaminating agents, as they could stain, or damage the lens surface and affect the quality of the
image.

Warning: Before connecting the microscope to a power source, make sure that the voltage coincides
with that of the microscope.

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OPERATION

A. Starting Up

1. Before using the microscope, adjust the light intensity control to minimum position. This should be
repeated every time the microscope is switched on or off to prolong the use of the bulb.

2. Switch to position ON.

3. Turn illumination control until the image is illuminated.

4. Light intensity must be adjusted according to the objective used, or the type of preparation to be
observed.

B. Interpupilliary adjustment of head.

1. Look through the eyepieces move the eyepiece holder tubes by rotating them on their axis.

2. When the field of vision is complete through both eyepieces, ie. that the two images unify into one,
interpupilliary distance is correct.

3. Each person may have to adjust interpupilliary distance to receive the same image.

C. Focussing the microscope.

1. Rotate the nosepiece and place the 10X objective in the optical path, making sure that it correctly
clicks into place.

2. Rotate the coarse focus knob until the stage rests in its lowest position.

3. Place a microscopic sample on the stage, make sure the cover slip faces upwards.

4. Swing the moveable finger on the mechanism, support the slide against the side holder and
gently release the moveable finger, until the slide is well supported.

5. Ensure that the sample on the slide is in the optical path, to do so, move the stage using the knobs
controlling the X/Y movement of the stage.

6. Looking through the eyepiece, turn the coarse focus knob until the preparation appears in focus.

7. Readjust the focus with the fine focus knob until the image appears sharply defined.

D. Adjusting diopter for differences in eyesight.

1. With your right eye, look into the right eyepiece tube and adjust the sharpness of the image using the
fine focus knob.

2. With your left eye, looking through the left eyepiece, adjust the focus by rotating the diopter corrector
on the left hand eyepiece tube until the image is sharp. Do not use the fine focus knob.

E. Critical illumination.

The ideal level of illumination when all illumination elements are brought into proportion, basically, by the
condenser and the field diaphragm.

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1. Focus on a sample with the 10X objective.

2. Close the field diaphragm turning the ring situated on the illuminator, until it appears in the field
of vision.

3. Next, focus the field diaphragm, moving the condenser using its focusing control. Do not use
the coarse or the fine focus control knobs.

NB: The focus of the field diaphragm is not completely sharp, although it can be adjusted to the maximum
possible.

4. Center the condenser using the locking and centering screws. As a consequence, the field
diaphragm will be centred.

5. Once field diaphragm is focused and centred, open the diaphragm just enough so that the field of vision
disappears. It must not be fully opened if optimum illumination is to be obtained.

NB: For each objective used, the field diaphragm must be opened to a different degree. If anything
irregular appears in the field of vision, an element of the illuminator or filter for example, appears in focus.
Move the condenser just enough to remove it from view.

F. Adjusting the opening diaphragm.

The opening diaphragm must not be used to regulate light intensity. Its function is to obtain the best
resolution possible of the object, and proportion best image contrast. The less the iris is opened, the
better the image contrast, although reducing the opening too much will worsen image contrast. The best
means of finding the best image contrast is to experiment with the size of the opening. Suggested
apertures (openings) are as follows:

Objective Iris Aperture

4X Totally closed to 1/8 open.

10X From 1/8 to 1/4
40X From 1/4 to 1/2
100X From 1/2 to 3/4

G. Changing magnification.

1. Position the 10X objective in the optical path.

2. This microscope arrives parfocalised, although it is possible that small differences exist between
objectives. If so, readjust slightly using the fine focus knob.

3. When the 40X and 100X objectives are changed, it must be done with extreme care, in particular making
sure that the objectives do not make contact with the slide as this could damage the objective front lens.

4. To obtain maximum resolution with the 100X objective, it is necessary to apply immersion oil between the
slide and the front lens of the objective.

a. Use a very small amount of immersion oil, a tiny drop should be enough.

b. If air bubbles appear, they can be removed by moving the revolving nosepiece slightly back and
forth.

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c. After using the microscope, all parts that have come into contact with immersion oil must be cleaned.
Using a soft cotton cloth lightly dampened with Xylene. If the 100X objective is not cleaned, oil could
dry on the lens surface resulting in the view being blocked, and possible damage occurring. This must
be repeated each time the microscope is used.

NB: Immersion oil must ONLY be used with the 100X objective, which is the only objective prepared
for it. If any other objective comes into contact with immersion oil, it must be cleaned immediately.

HOW TO ADAPT A PHOTOGRAPHIC, OR A VIDEO CAMERA. (Only for trinocular model)

Trinocular model comes equipped with a vertical port on the top of the head that permits the attachment of a
photographic, or reflex type camera through use of the corresponding adapters.

The two position sliding rod allows the microscope image to appear easily through this third path.

 When the rod is pushed completely into the head, 100% of the image is directed to the binocular
eyepiece for observation.

 In the extended position, 30% is directed to the binocular eyepiece, and 70% to the vertical port.

A. To adapt a photographic camera, an adapter tube is required. This tube includes a 2,5X lens for
photography which measures the parfocality correctly between the images of the binocular and vertical ports.
The adapter tube also requires a T mount adapted to fit the T threads of all photographic camera brands on
the market.

1. To install the camera on the microscope, first extract the removable lens on the camera, and place the
appropriate T thread. Then attach the adapter tube to the thread.

2. Loosen the knurled screw located on the side of the vertical port until the protective cap can be removed.

3. Insert the adapter tube with the camera already connected to the vertical port. If it does not fit easily,
unscrew the knurled screw until the adapter tube fits in and is firmly in place.

4. Tighten the knurled screw to secure the camera well.

5. Place the sliding rod to mid or extended position so that the image can be projected to the photographic
camera.

6. Operate the camera according to manufacturers instructions.

B. To adapt a video camera, an adapter tube is required. This adapter includes a 0.5X lens for
videos that measures the correct parfocality between the images of the binocular and vertical ports
shown as an image on the TV monitor. The adapter tube also includes a “C” knurled ring and a “CS” type
ring to accommodate different types of video cameras.

1. To install the camera to the microscope, screw the adapter tube to the video camera.

2. Loosen the knurled screw located on the side of the vertical port of the head, until the protective
cap can be removed.

3. Insert the adapter tube with the camera already connected to the vertical port. If it does not connect
easily, unscrew the knurled screw until the adapter tube fits in, and is secure in place.

4. Tighten the screw, to secure the camera.

5. Place the sliding rod, to mid or extended position to allow the image to be projected to the camera.

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6. Operate the camera according to the manufacturers instructions.

7. If the image on the TV monitor does not appear in focus when the objective is changed, this is possibly
due to the “CS” mount. Place it, or remove it, according to the procedure to obtain parfocality.

MAINTENANCE

WARNING: FOR YOUR SECURITY, SWITCH OFF AND REMOVE PLUG FROM POWER SOURCE
OUTLET BEFORE MAINTAINING YOUR MICROSCOPE IN ORDER TO AVOID SHOCK OR FIRE
HAZARD.

CONSULT YOUR DISTRIBUTOR IF YOUR MICROSCOPE REQUIRES ANY MAINTENANCE OR REPAIR

PROCEDURE NOT COVERED IN THIS INSTRUCTION MANUAL.

A. Optical Maintenance

1. Do not attempt to remove any optical component.

2. Before cleaning any lens, remove surface traces of dust using a fine brush, especially for lenses, or with
low pressure. Both can be obtained in any photography shop.

3. Cleaning the eyepiece.

a. Do not remove the eyepiece from the eyepiece tube.

b. Only clean the lens surface, misting the lens with breath.

c. Afterwards, dry the lens with special lens paper in circular movements, from center out to the exterior
of the lens. Do not wipe the lenses when dry, as they can be easily scratched.

4. Cleaning the objectives.

a. Do not remove the objectives from the microscope.

b. Only clean the surface area. Use a soft cotton cloth dampened with Xylene. Dry the lens using the
same cloth.

5. Cleaning the condenser.

a. Only clean the top lens surface of the condenser using any of the methods mentioned above for
cleaning the eyepieces or objectives.

6. Cleaning the illuminator lens.

a. Only clean the top lens of the illuminator using any of the methods mentioned above for cleaning
the eyepieces or objectives.

B. Electrical maintenance

WARNING: FOR YOUR SECURITY, SWITCH OFF AND REMOVE PLUG FROM POWER SOURCE
OUTLET BEFORE MAINTAINING YOUR MICROSCOPE IN ORDER TO AVOID SHOCK OR FIRE
HAZARD.

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1. Changing the bulb.

a. Lay the microscope on its side taking extreme care, especially with the eyepieces and the
specimen slide holder mechanism.

b. Unscrew the screw marked by the arrow.

c. Open the cap where the bulb is located.

d. With a cloth, carefully grasp the bulb and pull it out to disconnect it from the socket.

e. Do not touch the replacement bulb with fingers, use a clean cloth to insert the pins of the bulb into
the socket.

f. If bulb is touched accidently, it must be cleaned as this could affect the transmission of light and
duration of the bulb.

Replace the cap, and screw down firmly.

2. Changing the fuse.

a. With a flat screwdriver, press lightly on the slot on the fuse cap and turn 1/4 in the direction of
the arrow indicated.

b. Release pressure and the fuse cap should be able to be removed easily. Remove it completely.

c. Remove the fuse by pulling it out, and insert the new one. Ensure that a 0.5 amp fuse is being used.

d. Replace the fuse cap.

e. Repeat step (a.) this time 1/4 in the opposite direction of the arrow indicated. The cap should be well
closed.

C. Mechanical maintenance.

1. Adjusting the tension of the coarse focus control.

The tension adjustment control is located between the coarse focus knob and the arm .
The coarse focus nov comes pre-adjusted by the manufacturer. The ideal tension point is that which
permits coarse focus knob to move as loosely as possible, without the stage moving down on its own.

a. To tighten the focussing controls of the coarse focus knob, the ring must be turned in an anti-clockwise
direction, as indicated by the arrow. To loosen it, the ring must be turned clockwise.

2. Adjusting the stage stopper.

The 40X and 100X (optional) objectives incorporate security measure in that the lens tip retracts to avoid
damage to the front of the lens should it come into contact with the slide. An additional measure of
security consists of an adjustable stopper on the ascending movement of the stage. The stopper comes
pre-adjusted by the manufacturer for standard slides with cover slips 0.17mm thick. For observing other
types of samples, however, it may become necessary to readjust the stage stopper.

a. Loosen the stage stopper screw with a 2mm hexagonal key.

b. Focus on the sample using only the fine focus knob with firstly the 4X objective, and 10X objective.

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c. Tighten the stage stopper screw until firm enough for the stage is supported, and cannot ascend
further.

TROUBLESHOOTER

Electrical

Problem Cause Solution

Bulb does not work. Plug outlet does not work Repair by a qualified specialised
technician.
Cable not connected Connect cable.
Bulb burned out. Replace bulb.
Fuse blown. Replace fuse.
Wrong bulb. Replace by the correct bulb.
Bulb burns out in short time. Voltage too high. Reduce light intensity to a
minimum before turning the
microscope on or off.
Bulb burns out immediately. Wrong bulb. Replace with the correct bulb.
Bulb flickers. The bulb is not correctly inserted Insert correctly.
into the socket.
Bulb about to burn out. Replace bulb.
Fuse holder not locked into proper Close correctly.
position.
Loose connection at plug outlet. Repair by a qualified specialised
technician.
Fuse blows in short time. Wrong fuse. Replace with the appropriate fuse.
Fuse blows immediately. Short circuit Repair by a qualified technician.

Image Quality

Problem Cause Solution

No image. Nosepiece not positioned Turn until clicks into place.
properly.

Image too bright. Reduce the intensity of the light.

Poor resolution. Dirty objective. Clean objective.
Dirty eyepiece. Clean eyepiece.
Slide upside down. Replace the slide with the cover
slip facing upwards.
Wrong cover slip used with slide. Use 0.17mm thick cover slips.
Light too bright. Reduce light intensity or adjust the
diaphragm aperture.
Dirty condenser. Clean condenser.
Spots in field of view Dirty eyepiece. Clean eyepiece.
Dirty slide. Clean preparation.
Dirty condenser. Clean condenser.
Uneven illumination of field. Nosepiece not positioned Turn until it clicks into place.
properly.
Diaphragm aperture not Adjust appropriately.
sufficiently open.

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Mechanical

Problem Cause Solution

It does not stay in focus. The stage is sliding down on its Adjust the tension of the coarse
own. focus knob.
The stopper on the ascending
It does not focus. movement of the stage needs Readjust the stopper.
adjusting.

MOVING THE MICROSCOPE

 Avoid moving the microscope if possible.

 Carry the microscope in both hands, with one hand holding the arm, and the other supporting the
base.

 Keep the microscope in an upright position.

REPAIRS

If the microscope needs repairing, or revision by authorised personnel, we would recommend that it be
stored in its polystyrene box and returned to the distributor. Attach a note with a description of the problem,
or details of the required revision.

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