Biology Lab Report
Quarter 2 Laboratory Activity #2
Subject & Teacher: Biology 2, Mary Sheryl Saldon-Raznee
Group Number: 2
Group Members (Last name, First Name):
Adelante, Leila Loraine
Salomes, Godiva Bernice
Realiza, Aziraj Kent
Garcinces, Rhussel
Gelsano, Ethan
Tañan, Theojay Clarence
Laboratory Activity Name: Plant Cell Microscopy part II
Materials:
Microscope
Toothpicks/Cotton buds
Wet tissue
Surgical gloves
Methylene blue
Dropper
Glass and cover slips
Beaker
Pond water with microorganisms
Animal cells (procured from a subject’s mouth)
Algae
Procedure:
1.) Prepare glass slide and cover slip
2.) Double check if the cover slip is doubled with bare hands
3.) Wear surgical gloves
FOR THE FIRST ACTIVITY (Animal Cells):
4.) Using a dropper, put tiny drop methylene blue on the glass slide
5.) Procure animal cells
a. Dry a part of the cheeks inside the mouth with a towel or by airdrying (leaving
your mouth open for a minute)
b. Rub a toothpick or cotton bud along the inner cheek’s lining
6.) Blot the part of the toothpick which was used to collect cells on the area with
methylene blue on the glass slide
FOR THE SECOND ACTIVITY (Algae and Microorganisms):
4.) Select a small amount of algae from the given sample and extract it with a toothpick
5.) Place the algae on a glass slip and, after making sure that it makes a thin layer, cover it
with a cover slip
6.) Using a dropper, place a drop of the pond water which comes with the algae to seal the
coverslip
7.) Cover the sample with the cover slip
8.) Secure the glass slide with the sample on the microscope with the stage clips
9.) Turn on the microscope
10.) Start looking for the specimen with low power
11.) Locate the specimen using the course adjustment knob
12.) Locate the specimen using the stage adjustment knobs (for side-to-side movement)
13.) Repeat steps 11.) and 12.) for mid power
14.) Once a possible specimen has been located, switch to high power and repeat step 12.)
if needed
14.) Refine the resolution of the view of the specimen using the fine adjustment knob
15.) (Optional) Increase or decrease the lighting using the iris diaphragm for better
viewing of the specimen
16.) After finishing observing the specimen, clean the glass slides with gloves still on
17.) Leave glass slides on a safe place to air dry
18.) Turn off microscope
19.) Return microscope
20.) Clean working station
Results (post pictures and total resolution):
Animal Cells
160 magnification
Animal Cells Animal Cells
160 magnification 170 magnification
Analysis: (5pts each)
1.
Question: What are the 3 parts of the animal cell visible from your slide/s?
Answer: The parts of the animal cell seen from the microscope are the Cytoplasm, Plasma
membrane and the nucleus.
2.
Question: Why do we use methylene blue?
Answer: We use methylene blue to put more emphasis on the cells since it adds this pigment
that stains them. Without such, it would be transparent, resulting in an extremely harder time
locating what we were looking for.
3.
Question: How does the dye(methylene blue) work in staining the animal cell?
Answer: The dye itself stains the animal cell through the usage of positively charged dye
being attracted and staining the negatively charged particles/molecules within the cell
including the RNA and DNA.
4. .cv
Question: What microscopic organisms you found in the second activity? (list them and label
the images shown in the result section)
Answer: The microscopic organism we have found is most likely an arthropod, as stated by
the teacher.
5.
Question: Which domain do you think those microorganisms belong? (list them and label
their domain)
Answer: The domain which the aforementioned microorganism belongs to is most likely
Eukarya. Additionally, the green algae utilized in the experiment is part of the domain
Eukarya.
