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Vector Summary
Vector ID VB900102-9612yde
Vector Name pRP[Exp]-EGFP/Puro-CAG>hAPP[NM_000484.4]
Vector Size 8601 bp
Vector Type Mammalian Gene Expression Vector
Inserted Promoter CAG
Inserted ORF hAPP[NM_000484.4]
Inserted Marker EGFP/Puro
Plasmid Copy Number High
Antibiotic Resistance Ampicillin
Cloning Host VB UltraStable (or alternative strain)
Vector Map
Ampicillin
CAG
1
8000
pUC ori
Kozak
BGH pA 2000
VB900102-9612yde
6000 8601 bp
EGFP/Puro hAPP[NM_000484.4]
4000
CMV promoter SV40 late pA
Vector Components
Vector # : VB900102-9612yde Page 1 / 5
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Size
Name Position Type Description Application notes
(bp)
CMV early enhancer
fused to modified
CAG 22-1754 1733 Promoter Strong promoter.
chicken β-actin
promoter
Facilitates translation
Miscellaneo Kozak translation initiation of ATG start
Kozak 1779-1784 6
us initiation sequence codon downstream of the
Kozak sequence.
hAPP[NM_000
1785-4097 2313 ORF None None
484.4]
Allows transcription
termination and
Simian virus 40 late
SV40 late pA 4142-4363 222 PolyA_signal polyadenylation of mRNA
polyadenylation signal
transcribed by Pol II RNA
polymerase.
Human cytomegalovirus Strong promoter; may
CMV promoter 4367-4954 588 Promoter immediate early have variable strength in
enhancer/promoter some cell types.
Allows cells to be
visualized by green
EGFP/Puro 4986-6302 1317 ORF EGFP fused with Puro
fluorescence and
resistant to puromycin.
Allows transcription
termination and
Bovine growth hormone
BGH pA 6346-6570 225 PolyA_signal polyadenylation of mRNA
polyadenylation signal
transcribed by Pol II RNA
polymerase.
Facilitates plasmid
replication in E. coli;
pUC ori complement 589 Rep_origin pUC origin of replication regulates high-copy
(6766-7354) plasmid number (500-
700).
Ampicillin resistance Allows E. coli to be
Ampicillin complement 861 ORF
gene resistant to ampicillin.
(7525-8385)
Note: Components added by user are listed in bold red text.
Vector Sequence
1 CAACTTTGTA TAGAAAAGTT GCTCGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT
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81 AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
161 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT
241 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC
321 GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
401 CATGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA
481 TTTTTTAATT ATTTTGTGCA GCGATGGGGG CGGGGGGGGG GGGGGGGCGC GCGCCAGGCG GGGCGGGGCG GGGCGAGGGG
561 CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC AGAGCGGCGC GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG
641 GCGGCGGCGG CGGCCCTATA AAAAGCGAAG CGCGCGGCGG GCGGGAGTCG CTGCGCGCTG CCTTCGCCCC GTGCCCCGCT
721 CCGCCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT
801 CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTTG TTTCTTTTCT GTGGCTGCGT GAAAGCCTTG AGGGGCTCCG
881 GGAGGGCCCT TTGTGCGGGG GGAGCGGCTC GGGGGGTGCG TGCGTGTGTG TGTGCGTGGG GAGCGCCGCG TGCGGCTCCG
961 CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCAGTG TGCGCGAGGG GAGCGCGGCC
1041 GGGGGCGGTG CCCCGCGGTG CGGGGGGGGC TGCGAGGGGA ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC
1121 AGGGGGTGTG GGCGCGTCGG TCGGGCTGCA ACCCCCCCTG CACCCCCCTC CCCGAGTTGC TGAGCACGGC CCGGCTTCGG
1201 GTGCGGGGCT CCGTACGGGG CGTGGCGCGG GGCTCGCCGT GCCGGGCGGG GGGTGGCGGC AGGTGGGGGT GCCGGGCGGG
1281 GCGGGGCCGC CTCGGGCCGG GGAGGGCTCG GGGGAGGGGC GCGGCGGCCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC
1361 GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA
1441 AATCTGGGAG GCGCCGCCGC ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG
1521 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG ACGGCTGCCT
1601 TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG
1681 CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTGCAAGTT
1761 TGTACAAAAA AGCAGGCTGC CACCATGCTG CCCGGTTTGG CACTGCTCCT GCTGGCCGCC TGGACGGCTC GGGCGCTGGA
1841 GGTACCCACT GATGGTAATG CTGGCCTGCT GGCTGAACCC CAGATTGCCA TGTTCTGTGG CAGACTGAAC ATGCACATGA
1921 ATGTCCAGAA TGGGAAGTGG GATTCAGATC CATCAGGGAC CAAAACCTGC ATTGATACCA AGGAAGGCAT CCTGCAGTAT
2001 TGCCAAGAAG TCTACCCTGA ACTGCAGATC ACCAATGTGG TAGAAGCCAA CCAACCAGTG ACCATCCAGA ACTGGTGCAA
2081 GCGGGGCCGC AAGCAGTGCA AGACCCATCC CCACTTTGTG ATTCCCTACC GCTGCTTAGT TGGTGAGTTT GTAAGTGATG
2161 CCCTTCTCGT TCCTGACAAG TGCAAATTCT TACACCAGGA GAGGATGGAT GTTTGCGAAA CTCATCTTCA CTGGCACACC
2241 GTCGCCAAAG AGACATGCAG TGAGAAGAGT ACCAACTTGC ATGACTACGG CATGTTGCTG CCCTGCGGAA TTGACAAGTT
2321 CCGAGGGGTA GAGTTTGTGT GTTGCCCACT GGCTGAAGAA AGTGACAATG TGGATTCTGC TGATGCGGAG GAGGATGACT
2401 CGGATGTCTG GTGGGGCGGA GCAGACACAG ACTATGCAGA TGGGAGTGAA GACAAAGTAG TAGAAGTAGC AGAGGAGGAA
2481 GAAGTGGCTG AGGTGGAAGA AGAAGAAGCC GATGATGACG AGGACGATGA GGATGGTGAT GAGGTAGAGG AAGAGGCTGA
2561 GGAACCCTAC GAAGAAGCCA CAGAGAGAAC CACCAGCATT GCCACCACCA CCACCACCAC CACAGAGTCT GTGGAAGAGG
2641 TGGTTCGAGA GGTGTGCTCT GAACAAGCCG AGACGGGGCC GTGCCGAGCA ATGATCTCCC GCTGGTACTT TGATGTGACT
2721 GAAGGGAAGT GTGCCCCATT CTTTTACGGC GGATGTGGCG GCAACCGGAA CAACTTTGAC ACAGAAGAGT ACTGCATGGC
2801 CGTGTGTGGC AGCGCCATGT CCCAAAGTTT ACTCAAGACT ACCCAGGAAC CTCTTGCCCG AGATCCTGTT AAACTTCCTA
2881 CAACAGCAGC CAGTACCCCT GATGCCGTTG ACAAGTATCT CGAGACACCT GGGGATGAGA ATGAACATGC CCATTTCCAG
2961 AAAGCCAAAG AGAGGCTTGA GGCCAAGCAC CGAGAGAGAA TGTCCCAGGT CATGAGAGAA TGGGAAGAGG CAGAACGTCA
3041 AGCAAAGAAC TTGCCTAAAG CTGATAAGAA GGCAGTTATC CAGCATTTCC AGGAGAAAGT GGAATCTTTG GAACAGGAAG
3121 CAGCCAACGA GAGACAGCAG CTGGTGGAGA CACACATGGC CAGAGTGGAA GCCATGCTCA ATGACCGCCG CCGCCTGGCC
3201 CTGGAGAACT ACATCACCGC TCTGCAGGCT GTTCCTCCTC GGCCTCGTCA CGTGTTCAAT ATGCTAAAGA AGTATGTCCG
3281 CGCAGAACAG AAGGACAGAC AGCACACCCT AAAGCATTTC GAGCATGTGC GCATGGTGGA TCCCAAGAAA GCCGCTCAGA
3361 TCCGGTCCCA GGTTATGACA CACCTCCGTG TGATTTATGA GCGCATGAAT CAGTCTCTCT CCCTGCTCTA CAACGTGCCT
3441 GCAGTGGCCG AGGAGATTCA GGATGAAGTT GATGAGCTGC TTCAGAAAGA GCAAAACTAT TCAGATGACG TCTTGGCCAA
3521 CATGATTAGT GAACCAAGGA TCAGTTACGG AAACGATGCT CTCATGCCAT CTTTGACCGA AACGAAAACC ACCGTGGAGC
3601 TCCTTCCCGT GAATGGAGAG TTCAGCCTGG ACGATCTCCA GCCGTGGCAT TCTTTTGGGG CTGACTCTGT GCCAGCCAAC
3681 ACAGAAAACG AAGTTGAGCC TGTTGATGCC CGCCCTGCTG CCGACCGAGG ACTGACCACT CGACCAGGTT CTGGGTTGAC
3761 AAATATCAAG ACGGAGGAGA TCTCTGAAGT GAAGATGGAT GCAGAATTCC GACATGACTC AGGATATGAA GTTCATCATC
3841
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3921 AAAAATTGGT GTTCTTTGCA GAAGATGTGG GTTCAAACAA AGGTGCAATC ATTGGACTCA TGGTGGGCGG TGTTGTCATA
4001 GCGACAGTGA TCGTCATCAC CTTGGTGATG CTGAAGAAGA AACAGTACAC ATCCATTCAT CATGGTGTGG TGGAGGTTGA
4081 CGCCGCTGTC ACCCCAGAGG AGCGCCACCT GTCCAAGATG CAGCAGAACG GCTACGAAAA TCCAACCTAC AAGTTCTTTG
4161 AGCAGATGCA GAACTAGACC CAGCTTTCTT GTACAAAGTG GTGATGGCCG GCCGCTTCGA GCAGACATGA TAAGATACAT
4241 TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
4321 TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG
4401 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA
4481 ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
4561 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA
4641 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT
4721 TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
4801 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
4881 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG
4961 TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC
5041 TAACTAGAGA ACCCACTGCG CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
5121 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
5201 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT
5281 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA
5361 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
5441 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA
5521 TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC
5601 TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
5681 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT
5761 CGGCATGGAC GAGCTGTACA AGATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG
5841 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
5921 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT
6001 GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG
6081 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
6161 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG
6241 CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG
6321 CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACTCGAGTC TAGAGGGCCC
6401 GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT
6481 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT
6561 CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG
6641 GGCTCTATGG GCGGCCGCGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
6721 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
6801 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
6881 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
6961 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCTCTTCG GGAAGCGTGG CGCTTTCTCA
7041 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
7121 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
7201 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
7281 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
7361 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
7441 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
7521 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
7601 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
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7681 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
7761 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
7841 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
7921 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
8001 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
8081 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
8161 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
8241 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
8321 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
8401 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
8481 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT
8561 AGGCGTATCA CGAGGCCCTT TCGTCGGCGC GCCGCGGCCG C
Validation by Restriction Enzyme Digestion
Restriction Enzymes Cutting Sites DNA Fragments (bp)
NdeI 275, 4620 4345, 4256
SpeI 40, 4385 4345, 4256
ApaLI 7020, 8266 1246, 7355
EcoRI 3805 8601
DraIII 2118, 6275 4157, 4444
ApaLI+NdeI 275, 4620, 7020, 8266 4345, 2400, 1246, 610
ApaLI+SpeI 40, 4385, 7020, 8266 4345, 2635, 1246, 375
ApaLI+EcoRI 3805, 7020, 8266 3215, 1246, 4140
ApaLI+DraIII 2118, 6275, 7020, 8266 4157, 745, 1246, 2453
Vector # : VB900102-9612yde Page 5 / 5
