T E C H N I C A L N O T E

UV-Vis Spectroscopy

S23 Details and

Assessment,
Introduction
Configuration and The S20 Autosamplers were introduced in the
Installation SOP summer of 2020 as replacements for the old

for UV-VIS Products S10 autosampler.

Proprietary crash detection which prevents

damage from being caused to the autosampler probe if it comes into contact
with a capped sample vial. This reduces the turnover and cost of consumables
and ensures that your autosampler components are protected.

HIGHLIGHTS INCLUDE
n A chemically resistant sample tray which can be easily replaced if needed,
without having to replace the whole autosampler.
n LED status lighting, which provides a visible indication of the autosampler
state e.g. standby, running or error and ensures easy mitigation and
diagnosis of issues.
n The S20 Series Autosampler Software allows users to preset specific
parameters such as sampling speed, and autosampler peristaltic
pump speed thereby limiting the amount of programming used by the
respective software’s, allowing users full flexibility in their sampling needs.
n Once set, the S20 Software can be closed. The autosampler will simply need
a power cycle to retain the set parameters and offsets and does not require
to be open in the background, thereby further simplifying analysis.
n F
 ull control throughout all PKI spectroscopy software (Syngistix, UV Winlab™
and Spectrum FL).
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

Six Tray Configurations (Taken from Spectrum FL):

Figure 1. Five of the six configurations are shown here.

S20 Series Specifications: Shipping Kits:

Width Depth Height Weight There are two kits that will ship with the S20 autosamplers
Dimensions
(cm) (cm) (cm) (kg) when ordering either the S23 kit (N4100043) or the S25 kit
S23 57 53 45 9.5 (N4100044). The first kit is detailed below and includes the
autosampler itself along with the LAMBDA® 365+ specific parts.
S25 79 53 45 13.6
Enclosure S23 55.6 49.8 50.5 5.0 N4100043 Kit for S23 autosampler (N0830015) ship with the
following components:
Enclosure S25 76.8 49.8 50.5 5.5
Probe Arm Speed User-adjustable up to 330 mm/sec
Part
Pump Flow Rate User-adjustable up to 80 mL/min Part Description Qty
Number
Communication USB, serial RS-232 N0830015 System Autosampler S23 - 3 Rack 100 - 240v 1
Power Requirement 100-240 VAC, 47-63 Hz, 1.07 Amps N4101052 Flow Cell 1
Built-in Diagnostics Yes N4101054 Tubing Kit For Cell 1
N4101055 Tubing Kit For In-Built Sipper 1
B0199034 Violet/Violet Tubing 2.06 mm Id 1
Tube Outer Tube Height
Rack Type Rack Capacity N0811956 Carbon Support Probe 1.0 mm Id 1
Diameter (mm) (mm)
3 by 7 21-Position 28 115 N0830022 Fitting Kit (Required For Probe Fitting) 1
5 by 12 60-Position 17 100
6 by 15 90-Position 13 100

www.perkinelmer.com 2
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

N4100044 Kit for S25 autosampler (N0830025) ship with the B0199034 – 2.06 mm ID Tubing Ppl/Ppl/Ppl. Pkg. 12
following components: (Violet/Violet Tubing 2.06 mm ID)

B0193160 – 1.14 mm ID Tubing Red/Red/Red. Pkg. 12 (Red/

Part
Part Description Qty Red Tubing 1.14 mm ID)
Number
N0830025 System Autosampler S25 - 5 Rack 100 - 240v 1 n Used with tubing connection kit to make fittings with
N4101052 Flow Cell 1 flow‑through cell inlet and outlet tubing.
N4101054 Tubing Kit For Cell 1 n Used for connections with peristaltic pump.
N4101055 Tubing Kit For In-Built Sipper 1
B0199034 Violet/Violet Tubing 2.06 mm Id 1
N0811956 Carbon Support Probe 1.0 mm Id 1
N0830022 Fitting Kit (Required For Probe Fitting) 1

NOTE: Also included B0193160 - 1.14 mm ID Red/Red/Red Tubing. For

tubing connections between the autosampler probe, flow-through cell
and peristaltic pump.

The second kit is included with the autosampler itself and is

detailed below:
N0811956 – Carbon Fiber Sample Probe, 1.0 mm ID
Part (Carbon Support Probe 1.0 mm Probe Fitting)
Part Description Qty
Number
The 1.0 mm sample probe is shipped as default with the
N0811361 Usb Cable 1
S20 autosampler kit for L365+.
N0831034 Usb Flash Drive S20 Utility And User Guide 1
One Of Each
Power Supply: Autosampler S20 Series,
Cord Type
N0811381 Power Cords (Usa/Japan, Europe, Uk,
And One
Australia Pin Types)
Power Supply
N0777547 Carbon Support Probe 0.5 mm Id 1
Sample Tube W/ Screw Cap (50 ml) - Flat
B0193234 10 Tubes
Bottom
1 Rack
N0777167 Sample Tubes (15 ml)
(60 Tubes)
60 Position Sample Rack
N0830026 1
(For 15 ml Tubes)
N0831033 Rinse And Waste Tygon Tubing S20 Series 1
N0830022 Fitting Kit 1 N0830022 – Probe fitting kit for S20 series
N0831031 Peripump Tubing Id Pharmx 3.2 mm 1 (Fitting Kit (Required for Probe Fitting)
N0831030 Peripump Tubing Id Pharmx 2 mm 2 n Can be used as fittings for connections between the
B0104344 Narrow Neck Bottle 2000 ml w/o Cover 2 flow‑through cell inlet and outlet tubing, the sample probe
N0831032 Dual Rinse Station 1 and/or the peristaltic pump.

An example shipping list for an S23 is shown below:

NOTE: Shipping kit items may have a different description from those
listed above due to common part numbers being used for prior revisions.

www.perkinelmer.com 3
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

N4101052 – Flow-Through Cell 15.0 mm 420 µL, Quartz

(Flow Cell)

n Flow-through cell part number: N4101052

N4101055 – Front Plate Tube Connect Kit L365 Sipper

(Tubing Kit for In-Built Sipper)

N4101054 – Auto Sipper Tube Connection Kit (Tubing Kit for Cell)

n Includes the below parts.

n Tubing and conical adapters necessary for connecting the

sample probe to the flow-through cell.

n Inlet & outlet tubing are compatible with additional flow‑through

cells described in figure 2.

n LAMBDA 365+

Table 1: Flow-through Cells

Material Description Light Path Center Height Outside Dimensions Volume Part No.

Quartz SUPRASIL For use with LAMBDA 365/465 1 mm 15 mm 35 X 12.5 X 12.5 mm 62 μL B0631085

Quartz SUPRASIL For use with LAMBDA 365/465 10 mm 15 mm 35 X 12.5 X 12.5 mm 390 μL B0631084

Quartz SUPRASIL For use with LAMBDA 365/465 10 mm 15 mm 35 X 12.5 x 12.5 mm 160 μL B0631087

N4103018 Cell qtz suprasil ft 390 ul 10 mm pl pkg1 1

N4103019 Cell qtz suprasil ft 160 ul 10 mm pl pkg1 1

www.perkinelmer.com 4
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

Installing the S20 autosampler with a LAMBDA 365+.

NOTE: Make sure that the autosampler is switched off and that the power supply is disconnected until the S20 has been connected to the LAMBDA 365+.

1 Peristaltic Pump Configuration

The peristaltic pump tubing is pre-installed on the pump. There are three types of tubing available but the one that comes
pre‑installed is the ‘Tan Pharmed tubing’, PN: N0831030, Peripump Tubing ID PHARMX 2x2 mm. Figure 3 shows the tubing options
available for the S20 series.

Figure 2: Compatible peristaltic pump tubing options for the S20 series.

The peristaltic pump tubing adapters on channel 3 need to be

reversed to connect the outlet tube of the S20 flow-through cell.
The procedure to do this is detailed below:

1. Release the pressure shoe of Channel 3 by squeezing the

levers and pulling gently to the left.

Figure 3: Releasing the pressure shoe. Figure 4: Pressure shoe.

www.perkinelmer.com 5
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

2. If the pressure shoe moves a few centimeters and then 5. ‘Flip’ the fitting and plug the larger end of into the tube.
gets stuck, move your hand to the end of the pressure shoe
and continue pulling.

Figure 8: Reinstalling the fitting.

6. Reinstall the tube by sliding the clips on the end of the tube
into the slots in the pump. It is easiest to begin with the end
underneath the pump; stretch the tube slightly to reach the
slot on the top of the pump.
Figure 5: need figure info
7. At the outlet of the pump, connect a section of the Purple/
3. Lift the end of the tube straight up. Purple tube (B0199034). This section of tube can be cut
to an appropriate length. We will connect the outlet tube
of the flow-through cell to this section of tube using an
appropriate connector of which there are several options
(conical adapter, probe fitting kit, etc.).

2 Plumbing the Rinse Station

The rinse tubing should be connected to the rinse station and

peristaltic pump as per the configuration in Figure 9. The top
3 positions of the peristaltic pump are designated as ‘IN’, and
the bottom 3 positions are designated as ‘OUT’.

Figure 6: Removing the peristaltic pump tube.

4. Remove the fitting from both ends of the peristaltic

pump tube.

Figure 9: Single rinse tubing arrangement.

Figure 7: Removing the fitting from the peristaltic pump tube.

www.perkinelmer.com 6
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

Use the pre-cut Tygon tubing (N0831033) supplied with the S20 3 Installing the Sample Probe
to connect the rinse station to the peristaltic pump.

The correct configuration for the connection of the LAMBDA
UV-Vis and FL instruments is described as the ‘Pump after cell’
configuration and is not described in depth in the user guide
shipped with the S20. Figure 12 details this configuration
where the probe is directly connected to inlet tube of the
flow-through cell. The outlet tube of the flow-through cell is
connected to Channel 3 IN of the peristaltic pump.

Figure 10: Pre-cut Tygon tubing shipped with S20.

Figure 12: Pump after cell configuration flow diagram.

NOTE: To prevent contamination, wear gloves when handling the probe.

Figure 11: The rinse station (N0831032) is mounted into the station holder. Two of the
three short lengths of Tygon tubing connect the rinse station to the peristaltic pump. 1. Shut down and unplug the autosampler, if necessary.

2. Raise the slider on the Z-drive to its highest position.

The two pieces of short Tygon are attached to the peristaltic
pump as follows: 3. Route the sample tubing through the loop on top of the
Z-drive, as shown in figure 13.
1. Rinse station: Waste out – Channel 1, IN.
4. Loosen the two thumbscrews on the Z-drive slider.
2. Rinse station: Rinse in – Channel 2, OUT.
5. Guide the probe straight down through the sample holder
Cut an appropriate length of the long Tygon tubing to connect into the Z-drive slider.
‘Channel 1 OUT’ to one of the supplied narrow neck bottles
(B0104334). This will act as the waste reservoir for the
rinse solution.

Cut another length of the long tubing to connect to ‘Channel 2

IN’. This tube is for the rinse solution for the autosampler.

This generates 4 pieces of tubing (connections) with 2 more

required for Channel 3, which is reserved for the sample probe
and the flow-through cell.

Figure 13: Installing the sample probe.

www.perkinelmer.com 7
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

6. A
 djust the probe so that the tip of the probe is level with the 4 Connecting Flow-Through cell to the S20 Autosampler
bottom of the Y arm.

Figure 14: Adjusting the sample probe.

7. Tighten the thumbscrews (N0831035).

Figure 16: The sample probe tubing is coupled directly to the inlet tube of
the flow-through cell.

NOTE: Before cutting any tubing from either the sample probe or the
inlet/outlet tube of the flow be sure that there is sufficient tubing for the
Autosampler probe to move freely and without obstruction.
Figure 15: Tightening the sample probe thumbscrews.

Flow cell installation:

8. A
 lways position the sample tubing so that it does not pull
1. Remove the cell holder to reveal the space to route the inlet
on the probe. The tubing should naturally curve away from
and outlet tubing for the flow-through cell. There is a space
the probe so that it won’t rub or get caught. Be sure to
to the side of the compartment where the tubing can be
check that the tubing will not be stretched and will not snag
routed once the cell holder is reinstalled.
on an obstacle when the probe is moved to the far corner
sample positions.

CAUTION – Never attempt to move the sample probe by

hand during operation. The autosampler may be damaged
if the probe or autosampler arm are moved by hand when
the autosampler is under system control. Turn off the
autosampler if you need to manually move the arm or the
sample probe.

www.perkinelmer.com 8
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

2. Reinstall the single cell holder taking care not to pinch 2. To extend this tubing to the pump you will have to connect
the tubing. (You may choose to wait to do this until all multiple pieces together. To do this, cut a small piece (few
tubing is hooked up. This will allow you to pull tubing centimeters) of purple/purple (2.06 mm ID) peristaltic pump
through the bottom of the instrument to give you more to tubing. Connect the red/red/red peristaltic pump tube (1.14
play with while hooking the tubing up.) mm ID) to another red/red/red peristaltic pump tube using
that small piece of cut purple/purple peristaltic pump tubing.

3. T
 his second red/red/red peristaltic pump tubing gets
connected directly to the full purple/purple peristaltic pump
tubing that was installed onto Channel 3, IN of the peristaltic
pump during section 1, Peristaltic pump configuration, above.

3. The inlet tubing to the flow cell will come out the bottom of
the instrument. Cut a few centimeters of peristaltic pump
tubing (1.14 mm ID red/red/red). Fit this flow cell tubing
into one end of the piece of red/red/red peristaltic pump
tubing and the probe tubing from the autosampler into the
other end of the piece peristaltic pump tubing as shown
in Figure 17. Figure 19: Red/Red/Red tubing coming from connection with the flow cell
outlet tubing gets connected directly into the Purple/Purple tubing connected
to Channel 3, IN (right).

5 Software Installation and Configuration

NOTE: The S20 software, installation files and user guides are all stored
on the USB included with the shipping kit (N0831034).

1. Be sure to select and install the autosampler configuration

during the installation of UV Winlab or modify the
installation to install the autosampler accessory.
Figure 17: Connection between the probe tubing and Flow cell inlet tubing
(under the instrument) is made using a small piece of red/red/red tubing. 2. Install the S20 autosampler software from the USB that
ships with the autosampler. This software can be used to
Connecting the Flow-Through Cell Outlet to the Peristaltic Pump perform the autosampler qualification as well as modify
certain parameters of the autosampler such as the
1. The outlet tubing from the flow cell will also come out the
peristaltic pump speed.
bottom of the instrument. Connect this tubing directly into
the peristaltic pump tubing (1.14 mm ID red/red/red) as seen 3. The COM port assigned to the autosampler can also be
in Figure 18. confirmed in the S20 software, COM 8 is assigned in the
below example.

NOTE: The S20 software can also be used to adjust parameters such as
the pump speed which can be optimized as per the requirement of the
application being used. For further details regarding the S20 software
please consult the S23/S25 Autosampler guide supplied on the USB from
Figure 18: Union between sample probe and flow-through cell inlet tube.
the shipping kit (N0831034).

www.perkinelmer.com 9
S23 Details and Assessment, Configuration and Installation SOP for UV-VIS Products

6. Once the correct COM port has been you will be able to take
control of the autosampler after selecting it in the ‘Accessories’
tab of the Method or Task you are working with.

4. Proceed to UV Winlab software and select the ‘Tools’ tab

and then the ‘Accessories Management’ tab.

7. Parameters set in the ‘Autosampler’ tab (fill time, delay time,

flush/sample return time and autozero sample position),
will be saved as default for the method. This allows the
relevant column to be set as default in the sample list to
use the values for the parameters entered
in the ‘Autosampler’ tab.

5. T
 his will open the ‘Accessories Properties’ window and will
allow you to select the correct COM port for the autosampler.

PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright ©2023, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

794202 PKI