TripleTOF® 4600 System
Planned Maintenance Procedure
RUO-IDV-09-1897-D September 2017
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Contents
Chapter 1 Introduction...............................................................................................................................4
Chapter 2 Planned Maintenance Tasks......................................................................................................5
Pre-Planned Maintenance..................................................................................................................................................5
Pre-PM Tasks...............................................................................................................................................................5
Pre-PM System Tests....................................................................................................................................................6
Planned Maintenance......................................................................................................................................................10
Mass Spectrometer Maintenance...............................................................................................................................10
TM
DuoSpray Ion Source Maintenance........................................................................................................................11
Post-Planned Maintenance..............................................................................................................................................11
Voltage Tests.............................................................................................................................................................11
Pressure Test..............................................................................................................................................................12
Post-PM System Tests................................................................................................................................................12
Post-PM Tasks............................................................................................................................................................20
Review.......................................................................................................................................................................20
Chapter 3 Signoff......................................................................................................................................22
Comments and Exceptions...............................................................................................................................................23
Appendix A Inspect for Contamination...................................................................................................24
Guidelines for Identifying Components to be Cleaned.....................................................................................................24
Planned Maintenance Procedure TripleTOF® 4600 System
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Introduction 1
Note: For regulatory and safety information for the mass spectrometer, refer to the System User Guide.
The planned maintenance (PM) procedure is designed to help maintain overall system performance.
The PM is not intended to take the place of an Operational Qualification (OQ) nor is it intended to verify the
instrument specifications. Separate Installation Qualification (IQ) and OQ services are available. Contact a SCIEX
representative.
The procedure must be performed by a trained SCIEX Field Service Employee (FSE).
® TM
The procedure has been developed for the TripleTOF 4600 system with the DuoSpray ion source. It does not
apply to any other products or processes.
®
These procedures can also be performed using a NanoSpray ion source.
The procedure does not address any customer-specific analytical protocol (performance qualification) or method
validation.
Note: If an issue is identified and the system requires repair, then the customer is responsible for the repair at
the expense of the customer, except to the extent that the system and the required repairs are covered by a
SCIEX warranty or service contract. A separate repair service call must be opened and the repair hours must not
be charged against this procedure.
TripleTOF® 4600 System Planned Maintenance Procedure
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Planned Maintenance Tasks 2
Indicate the ion source used for the maintenance procedures:
TM ®
DuoSpray Ion Source NanoSpray Ion Source
Pre-Planned Maintenance
Note: Guideline values are for reference only. The pre-PM test results are not required to meet or exceed these values.
Pre-PM Tasks
Task Complete
Ask the customer about system performance since the last visit and record comments.
If the customer maintains a log for the system, then review it.
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
Task Complete
Review the work to be performed with the customer.
Inspect for front-end contamination. Refer to Inspect for Contamination on page 24.
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Planned Maintenance Tasks
Pre-PM System Tests
The Q1 Positive PPGs Test is Complete
-4
• Test solution: POS PPG, 1 × 10
• Cycles: 25
• Step Size: 0.1 Da
• MCA: On
• Printouts required: Spectra for masses 59.05, 175.13, 442.34, and 674.50, 906.67, and 1196.88, with peak intensities, peak width, and mass shift
results, complete with method file information.
Mass (Da) Intensity (cps) Peak width (Da)
Guideline Result Guideline Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
5 4
59.05 1.5 × 10 7.5 × 10 0.6 to 0.8
4 3
175.13 1.0 × 10 5.0 × 10 0.6 to 0.8
5 4
442.34 1.8 × 10 9.0 × 10 0.6 to 0.8
5 4
674.50 1.3 × 10 6.5 × 10 0.6 to 0.8
5 4
906.67 5.0 × 10 2.5 × 10 0.6 to 0.8
4 4
1196.88 5.0 × 10 52.5 × 10 0.6 to 0.85
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
The Q1 Negative PPGs is Complete
-4
• Test solution: POS PPG, 1 × 10
• Cycles: 25
• Step Size: 0.1 Da
• MCA: On
• Printouts required: Spectra for masses 45.00, 411.26, 585.39, 933.64, and 1165.80, with peak intensities, peak width, and mass shift results,
complete with method file information.
Mass (Da) Intensity (cps) Peak width (Da)
Guideline Result Guideline Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
4 4
933.64 8.0 × 10 4.0 × 10 0.6 to 0.8
3 3
1165.80 7.0 × 10 3.5 × 10 0.6 to 0.85
TripleTOF® 4600 System Planned Maintenance Procedure
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Planned Maintenance Tasks
The Positive Product Ion Test is Complete
• Test solution: Reserpine 0.167 pmol/µL (Reserpine Ref.)
• Accumulation time: 1.0 sec
• Cycles: 30
• MCA: On
• ITC: 100%
• Printouts required: Spectra for masses 174.0913, 195.0652, 397.2122, 448.1966, with peak intensities, peak width, and mass shift results, complete
with method file information.
Mass (Da) Resolution Centroid Intensity (cps)
Guideline Result Guideline Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
174.0913 N/A N/A 20 000 10 000
195.0652 N/A N/A 30 000 15 000
397.2122 25 000 6 500 3 250
448.1966 N/A N/A 5 000 2 500
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
Planned Maintenance
Mass Spectrometer Maintenance
Task Complete N/A
Shut down the system and then disconnect the mains supply power cable.
If required, replace the roughing pump oil. The recommended interval is every 12 months.
Note: If an oil change is not required, then inspect the oil level and top up, if necessary.
CAUTION: Potential System Damage. Do not mix different types of oil. Mixing mineral
oil with synthetic oil can cause pump failure.
If required, replace the roughing pump oil exhaust filter. The recommended interval is every 24 months.
Clean or replace the mass spectrometer cooling fans filters.
(If applicable) Verify the operation of the cooling fans in the roughing pump enclosure.
Inspect the mass spectrometer, components, and cabling, as required.
Clean the curtain plate.
Clean the orifice plate.
®
Clean the QJet ion guide and IQ0 lens.
(If contamination is detected) Clean the Q0 region.
Start up the system.
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Planned Maintenance Tasks
TM
DuoSpray Ion Source Maintenance
Task Complete N/A
®
If necessary, replace the electrode in the TurboIonSpray and the APCI probe.
Verify that the temperature (TEM) reaches a set point of 200 °C.
Post-Planned Maintenance
Task Complete
Inspect for front-end contamination. Refer to Inspect for Contamination on page 24.
Voltage Tests
Task Complete
Inspect the RF tuning voltages at the QPS amplifier module (Q1 and Q2), and then, if required, tune the coil boxes.
Inspect the detector voltage, optimizing if necessary.
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
Pressure Test
The Post-PM Pressure Test is Complete
Test Guideline Result
–5
Quad pressure with CAD gas off 3.0 × 10 torr
–6
TOF pressure with CAD gas off 2.0 × 10 torr
–5
Quad pressure with CAD gas set to Full 5.0 × 10 torr to 8.0 ×
–5
10 torr
–6
TOF pressure with CAD gas set to Full 2.0 × 10 torr
TripleTOF® 4600 System Planned Maintenance Procedure
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Planned Maintenance Tasks
Post-PM System Tests
The Q1 Positive PPGs Test is Complete
-4
• Test solution: POS PPG, 1 × 10
• Cycles: 25
• Step Size: 0.1 Da
• MCA: On
• Printouts required: Spectra for masses 59.05, 175.13, 442.34, and 674.50, 906.67, and 1196.88, with peak intensities, peak width, and mass shift
results, complete with method file information.
Mass (Da) Intensity (cps) Peak width (Da)
Specification Result Specification Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
5 4
59.05 1.5 × 10 7.5 × 10 0.6 to 0.8
4 3
175.13 1.0 × 10 5.0 × 10 0.6 to 0.8
5 4
442.34 1.8 × 10 9.0 × 10 0.6 to 0.8
5 4
674.50 1.3 × 10 6.5 × 10 0.6 to 0.8
5 4
906.67 5.0 × 10 2.5 × 10 0.6 to 0.8
4 4
1196.88 5.0 × 10 52.5 × 10 0.6 to 0.85
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
The Q1 Negative PPGs Test is Complete
-4
• Test solution: POS PPG, 1 × 10
• Cycles: 25
• Step Size: 0.1 Da
• MCA: On
• Printouts required: Spectra for masses 45.00, 411.26, 585.39, 933.64, and 1165.80, with peak intensities, peak width, and mass shift results,
complete with method file information.
Mass (Da) Intensity (cps) Peak width (Da)
Specification Result Specification Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
4 4
933.64 8.0 × 10 4.0 × 10 0.6 to 0.8
3 3
1165.80 7.0 × 10 3.5 × 10 0.6 to 0.85
TripleTOF® 4600 System Planned Maintenance Procedure
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Planned Maintenance Tasks
The Negative TOF MS Test is Complete
–4
• Test solution: POS PPG, 1 × 10
• Accumulation time: 1.0 sec
• Cycles: 30
• MCA: On
• ITC:
• Printouts required: Spectra for mass 933.6366, with peak intensities, peak width, and mass shift results, complete with method file information.
Mass (Da) Resolution
Specification Result
933.6366 25 000
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
The Positive TOF MS Test is Complete
• Test solution: Tuning Solution (CsI ALILTLVS Reference)
• Accumulation time: 1.0 sec
• Cycles: 30
• MCA: On
• ITC:
• Printouts required: Spectra for mass 829.5393, with peak intensities, peak width, and mass shift results, complete with method file information.
Mass (Da) Resolution
Specification Result
829.5393 25 000
TripleTOF® 4600 System Planned Maintenance Procedure
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Planned Maintenance Tasks
The Positive Product Ion Test is Complete
• Test solution: Reserpine 0.167 pmol/µL (Reserpine Ref.)
• Accumulation time: 1.0 sec
• Cycles: 30
• MCA: On
• ITC: 100%
• Printouts required: Spectra for masses 174.0913, 195.0652, 397.2122, 448.1966, with peak intensities, peak width, and mass shift results, complete
with method file information.
Mass (Da) Resolution Centroid Intensity (cps)
Specification Result Specification Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
174.0913 N/A N/A 20 000 10 000
195.0652 N/A N/A 30 000 15 000
397.2122 25 000 6 500 3 250
448.1966 N/A N/A 5 000 2 500
Planned Maintenance Procedure TripleTOF® 4600 System
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Planned Maintenance Tasks
The Negative Product Ion Test is Complete
• Test solution: Taurocholic Acid 2 ng/µL (Taurocholic Acid)
• Accumulation time: 1.0 sec
• Cycles: 30
• MCA: On
• ITC: 100%
• Printouts required: Spectra for masses 79.9574, 106.9808, 124.0074, 514.2844, with peak intensities, peak width, and mass shift results, complete
with method file information.
Mass (Da) Resolution Centroid Intensity (cps)
Specification Result Specification Result
TM ®
DuoSpray Ion NanoSpray Ion
Source Source
79.9574 N/A N/A 35 000 17 500
514.2844 25 000 15 000 7 500
Fragments are observed at m/z 79.9574, 106.9808, and 124.0074.
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Planned Maintenance Tasks
TM
The DuoSpray Ion Source Heater Test is Complete Complete N/A
Test Specification Result (Passed)
®
Set the temperature parameter for the The Analyst TF software reports that the temperature is reached.
TIS probe to 500 °C.
®
Set the temperature parameter for the The Analyst TF software reports that the temperature is reached.
APCI probe to 400 °C.
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Planned Maintenance Tasks
Post-PM Tasks
Task Complete N/A
®
(Obtain customer approval first) Install any applicable Analyst TF software HotFixes.
Delete any unnecessary files.
Back up the Analyst Data folder.
(If applicable) Defragment the hard drive.
Inspect the status of the RAID hard drives.
If the customer has a Software Support Plan, then perform the Software Health Check:
• (Obtain customer approval first) Install any compatible HotFixes and updates for SCIEX add-on software.
®
Log on to the StatusScope server using the FSE user name and then verify that the assets under the customer
®
organization are connected to the StatusScope server.
Perform the post-PM fault test to verify that the mass spectrometer fault generated is shown in the
®
StatusScope software.
Review
Task Complete
Review the work performed with the customer.
Record the verification data in this document and then attach all of the performance data.
Review the verification data for the completed maintenance with the customer.
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Planned Maintenance Tasks
Task Complete
Review the routine maintenance schedule and the procedures with the customer.
Make a copy of this completed document and attach all performance data. Then:
• Obtain customer acceptance.
• Provide the customer with the original planned maintenance document and the performance data.
Planned Maintenance Procedure TripleTOF® 4600 System
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Signoff 3
Organization
Mass Service request
spectrometer number
serial number
Customer contact Date
name (yyyy-mm-dd)
Customer contact
signature*
FSE name Date
(yyyy-mm-dd)
FSE signature
* Signature required on hard copy only.
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Signoff
Comments and Exceptions
Planned Maintenance Procedure TripleTOF® 4600 System
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Inspect for Contamination A
1. Run the Q1 Pos PPG method for 10 minutes, monitoring the TIC for degradation of the signal or sensitivity
drops.
2. Change the polarity to negative, and then scan for one minute.
3. Change the polarity to positive, and then make sure that the ISVF parameter returns to the original value.
4. Run the method.
If the signal sensitivity is restored temporarily and then it starts to degrade again, then the charging effect is
present.
Guidelines for Identifying Components to be
Cleaned
If the Q1 quadrupole is showing the charging effect and signal drop out, then clean the Q0 and the vacuum
®
interface components (the curtain plate, orifice plate, the QJet ion guide, and the Q0 side of the 1Q1 lens) using
the customer cleaning procedure, with the rod and tissues. In most cases, this will remove the contamination.
Notes:
• Do not remove the ion optics during a PM. This is considered to be a repair activity, and requires a separate
service call to be opened.
• Consider cleaning the Q1 quadrupole as well as other components (the stubbies and the 1Q1 lens), if cleaning
the front end does not eliminate the contamination.
• This guideline does not provide complete troubleshooting for all possible technical root causes of the signal
®
degradation or charging effect. Signal degradation might also result from a contaminated TurboIonSpray
probe or electrode, method parameters that are not optimized, and so on.
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