ACQUITY UPLC

Systems with 2D
Technology
Capabilities Guide
715004391/Revision B

Copyright © Waters Corporation 2015

All rights reserved
September 25, 2015, 715004391 Rev. B
Page ii
General Information

Copyright notice

© 2013–2015 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF

AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS
THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN
PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should not
be construed as a commitment by Waters Corporation. Waters Corporation assumes
no responsibility for any errors that may appear in this document. This document is
believed to be complete and accurate at the time of publication. In no event shall
Waters Corporation be liable for incidental or consequential damages in connection
with, or arising from, its use. For the most recent revision of this document, consult
the Waters Web site (waters.com).

Trademarks
®
ACQUITY is a registered trademark of Waters Corporation.
®
ACQUITY UPLC is a registered trademark of Waters Corporation.
®
Empower is a registered trademark of Waters Corporation.

MassLynx® is a registered trademark of Waters Corporation.

UPLC® is a registered trademark of Waters Corporation.

®
“THE SCIENCE OF WHAT’S POSSIBLE.” is a registered trademark of Waters
Corporation.

Waters® is a registered trademark of Waters Corporation.

Waters Quality Parts® is a registered trademark of Waters Corporation.

®
MP35N is a registered trademark of SPS Technologies.

All other trademarks or registered trademarks are the sole property of their respective
owners.

September 25, 2015, 715004391 Rev. B

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Customer comments

Waters’ Technical Communications organization invites you to report any errors that
you encounter in this document or to suggest ideas for otherwise improving it. Help
us better understand what you expect from our documentation so that we can
continuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at

[email protected].

Contacting Waters

Contact Waters with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us via the
Internet, telephone, or conventional mail.

Waters contact information

Contacting medium Information

Internet The Waters Web site includes contact information for
Waters locations worldwide. Visit www.waters.com.
Telephone and fax From the USA or Canada, phone 800-252-4752, or fax
508-872-1990.
For other locations worldwide, phone and fax numbers
appear in the Waters Web site.
Conventional mail Waters Corporation
Global Support Services
34 Maple Street
Milford, MA 01757
USA

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose
chemical, biological, or radiological hazards (or any combination thereof). You must
know the potentially hazardous effects of all substances you work with. Always follow
Good Laboratory Practice, and consult your organization’s standard operating
procedures.

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is used to

find out the nature of the potential hazard and any actions which have to be taken.

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Power cord replacement hazard

Warning: To avoid electric shock, use the SVT-type power cord in the United
States and HAR-type (or better) cord in Europe. The main power cord must be
replaced only with one of adequate rating. For information regarding what cord
to use in other countries, contact your local Waters distributor.

Hand crush hazard

Warning: To avoid hazards associated with the reciprocating or rotating parts

in the source, keep hand clear of the regions marked with yellow and gray
labels.

High voltage hazard

Warning: To avoid electric shock, observe these precautions:

• Do not remove the mass spectrometer’s protective panels. The components
they cover are not user-serviceable.
• When the instrument is in Operate mode, avoid touching the areas marked
with the high voltage warning symbol. To touch external areas marked with
the symbol, first put the instrument in Standby mode.

Bottle placement prohibition

Warning: To avoid injury from electrical shock or fire, and damage to the
equipment, do not place vessels containing liquid atop the workstation or ancillary
equipment or otherwise expose those units to dripping or splashing liquids.

Prohibited: Do not place vessels containing liquid—such as solvent

bottles—atop the workstation or ancillary equipment or otherwise expose those
units to dripping or splashing liquids.

FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for
compliance, could void the users authority to operate the equipment. This device
complies with Part 15 of the FCC Rules. Operation is subject to the following two
conditions: (1) this device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause
undesired operation.

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Electrical power safety notice
Do not position the instrument so that it is difficult to disconnect the power cord.

Equipment misuse notice

If equipment is used in a manner not specified by its manufacturer, protections
against personal injury inherent in the equipment’s design can be rendered
ineffective.

Safety advisories
Consult Appendix A for a comprehensive list of warning advisories and notices.

Operating the system

When operating the system, follow standard quality-control (QC) procedures and the
guidelines presented in this section.

Applicable symbols

Symbol Definition
Manufacturer

Date of manufacture

Authorized representative of the European Community

Confirms that a manufactured product complies with all

applicable European Community directives

Australia EMC compliant

or

Confirms that a manufactured product complies with all

applicable United States and Canadian safety
requirements
Consult instructions for use

Alternating current

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 Symbol Definition
Electrical and electronic equipment with this symbol may
contain hazardous substances and should not be disposed
of as general waste.
For compliance with the Waste Electrical and Electronic
Equipment Directive (WEEE) 2012/19/EU, contact Waters
Corporation for the correct disposal and recycling
instructions.
Serial number

Part number catalog number

5()

Audience and purpose

Waters prepared this document describing the features and specifications of ACQUITY
2D Technology for both novice and experienced users.

Intended use of ACQUITY UPLC Systems with 2D

Technology
Waters designed ACQUITY UPLC Systems with 2D Technology to be used to perform
chromatographic separations. These systems are for research use only and are not
intended for use in diagnostic applications.

Calibrating
To calibrate LC systems, follow acceptable calibration methods using at least five
standards to generate a standard curve. The concentration range for standards must
include the entire range of QC samples, typical specimens, and atypical specimens.

Quality control
Routinely run three QC samples that represent subnormal, normal, and above-normal
levels of a compound. If sample trays are the same or very similar, vary the location
of the QC samples in the trays. Ensure that QC sample results fall within an
acceptable range, and evaluate precision from day to day and run to run. Data
collected when QC samples are out of range might not be valid. Do not report these
data until you are certain that the instrument performs satisfactorily.

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EMC considerations

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-001.

Cet appareil numérique de la classe A est conforme à la norme NMB-001.

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with IEC CISPR 11 Industrial
Scientific and Medical (ISM) instruments requirements.

Group 1 products apply to intentionally generated and/or used conductively coupled

radio-frequency energy that is necessary for the internal functioning of the
equipment.

Class B products are suitable for use in both commercial and residential locations and
can be directly connected to a low voltage, power-supply network.

EC authorized representative

Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK

Telephone: +44-161-946-2400
Fax: +44-161-946-2480
Contact: Quality manager

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Table of Contents
General Information .................................................................. iii
Copyright notice ......................................................................................... iii

Trademarks ................................................................................................ iii

Customer comments ................................................................................... iv

Contacting Waters ...................................................................................... iv

Safety considerations .................................................................................. iv

Safety hazard symbol notice.............................................................. iv
FCC radiation emissions notice........................................................... v
Electrical power safety notice............................................................. vi
Equipment misuse notice .................................................................. vi
Safety advisories ............................................................................. vi

Operating the system .................................................................................. vi

Applicable symbols........................................................................... vi
Audience and purpose ..................................................................... vii
Intended use of ACQUITY UPLC Systems with 2D Technology ................ vii
Calibrating ..................................................................................... vii
Quality control................................................................................ vii

EMC considerations ................................................................................... viii

Canada spectrum management emissions notice ................................ viii
ISM Classification: ISM Group 1 Class B ............................................ viii

EC authorized representative ...................................................................... viii

1 ACQUITY UPLC System with 2D Technology ............................. 13

1.1 Overview ................................................................................................. 13

2 Configuring the System ............................................................ 15

2.1 Configuring the system in Empower 3 software ............................................. 15

2.2 Configuring the system in MassLynx software ............................................... 16

2.3 Configuring Waters Pump Control software ................................................... 17

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 2.4 Configuring the CM-A ................................................................................ 18

2.5 Connecting external valves to the CM-A ....................................................... 20

3 System Components and Features ........................................... 23

3.1 Description of ACQUITY UPLC System with 2D Technology .............................. 23
3.1.1 Column manager (CM-A) .................................................................. 24
3.1.2 Solvent manager ............................................................................. 26
3.1.3 Additional pumps............................................................................. 26
3.1.4 External valves ............................................................................... 26
3.1.5 Tubing ........................................................................................... 27

4 Example System Configurations ............................................... 29

4.1 Flow path configurations ............................................................................ 29
4.1.1 Trap and back-flush elution configuration ............................................ 29
4.1.2 Trap and back-flush elution with at-column dilution (ACD) ..................... 31
4.1.3 Trap and forward-flush elution configuration ........................................ 32
4.1.4 Valve positions ................................................................................ 33
4.1.5 Heart-cutting configuration ............................................................... 34
4.1.6 Parallel column regeneration configuration .......................................... 35

4.2 Comprehensive 2D UPLC ........................................................................... 37

5 Spare Parts .............................................................................. 39

5.1 List of Spare Parts .................................................................................... 39

6 Worksheets .............................................................................. 41
6.1 Worksheets .............................................................................................. 41
6.1.1 Blank worksheets ............................................................................ 42
6.1.2 Completed worksheets ..................................................................... 45

A Safety Advisories ..................................................................... 49

A.1 Warning symbols ...................................................................................... 49
A.1.1 Specific warnings ............................................................................ 50

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A.2 Notices ................................................................................................... 51

A.3 Bottles Prohibited symbol .......................................................................... 52

A.4 Required protection .................................................................................. 52

A.5 Warnings that apply to all Waters instruments and devices ............................. 52

A.6 Warnings that address the replacing of fuses ................................................ 55

A.7 Electrical and handling symbols .................................................................. 57

A.7.1 Electrical symbols............................................................................ 57
A.7.2 Handling symbols ............................................................................ 58

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1 ACQUITY UPLC System with
2D Technology

This chapter gives an overview of ACQUITY UPLC with 2D technology.

1.1 Overview

Waters ACQUITY UPLC® Systems with 2D Technology employ solvent managers

(pumps), a sample manager, a column manager, optical detectors, mass
spectrometers, and a wide range of separation chemistries to facilitate
multidimensional analyses. To meet the specific needs of your application, you can
configure a 2D system that includes as many as three ACQUITY UPLC solvent
managers and as many as three 515 pumps (or one 1525 pump). The column
manager (CM-A), which is a required part of all 2D systems, incorporates
programmable switching valves, and two, independent heating zones with active
preheating. Various core systems, all in the UPLC® family, are available as foundations
for 2D technology.

Tip: You can upgrade a conventional ACQUITY UPLC system to include 2D technology.

Using a system with 2D technology, you can achieve these goals:

• Eliminate matrix effects

• Improve peak capacity (gain selectivity and sensitivity)
• Improve ruggedness (cleaner MS source, extended column lifetime)
• Obtain higher quality data for decision making

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2 Configuring the System

You must configure the system to operate under the control of Empower or MassLynx
software. You must also configure the Waters Pump Control software, and connect
external valves to the CM-A.

Important:

• To enable 2D features, you must operate the CM-A in Advanced mode.

• For information about the configuration of the pump and sample manager, other
than those listed in this document, contact Waters Technical Support.
• When operating any system that includes multiple pumps and valves, you must
know the pressure limitations of all system components including flow cells,
pumps, valves, and columns. Moreover, you must consider pressures at all
stages of the method during system operation, mindful that configurations and
operations vary in the level of pressure they produce within a system and that
the pressure on individual components within systems can vary.
• When you add or replace an instrument component of an ACQUITY UPLC
System with 2D Technology, repeat the procedure for configuring the system,
below. Proper communication cannot be achieved unless you repeat the
configuration procedure for the new instrument.

2.1 Configuring the system in Empower 3 software

Note: To use 2D technology under Empower control, you must use Empower 3
software together with Driver Pack 4.

To configure the system in Empower software:

1. Open Empower software, and select Configure System.
2. Select Systems > New > Chromatographic System.
3. In the New Chromatographic System Wizard, drag the modules that you want to
include in the system from the Available Instruments list to the New System
Instruments list, and then click Next.
4. Select the desired access under Allowed Access, and then click Next.
5. Type the name of the system in the System Name box, and then click Finish.

For systems under the control of Empower software, you must select the binary
solvent manager to be used as the system’s primary flow source.

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 To configure the system’s primary flow source:
1. From Run Samples in Empower 3, open the system Console.
2. In the console software, select Configure > Primary flow source, and then select
the binary solvent manager that you intend to use.

2.2 Configuring the system in MassLynx software

Mass Lynx-controlled 2D technology requires the use of MassLynx software with

Driver Pack 3 or Driver Pack 4.

To configure the system in MassLynx software:

1. Verify that all necessary instrument cables are connected, and that all
instruments are powered-on.
2. Open MassLynx software, and select Inlet Method.
3. Select Tools > Instrument Configuration, to open the Inlet Configuration dialog
box.
4. Click Configure, to open the wizard, and then click Next on the wizard‘s
Welcome page.
5. On the Select Pump page, choose Waters Pump Control, and then click Next.
6. On the Select Auto Samples page, select the applicable Sample Manager, and
then click Next.
7. On the Select Detectors page, select Waters ACQUITY CM, and use the
right-hand arrow to drag the selected detector to the Configured Detectors area
of the page.
8. Sequentially, select each applicable detector, drag it to the Configured Detectors
area, and then click Next.
9. On the Configured Communications page, click Scan for Instruments.

10. Verify that the list of ACQUITY® modules that appears in the Waters Instrument
Scan dialog box is correct, and then click OK.
11. In the box that corresponds to each instrument, click the list, then select the
instrument’s ID, and then click Next.
12. When the “Configuration Successful” prompt appears, click Finish.
13. If any of the detectors (including the column manager) do not appear in the
Selected Configuration Addresses section, repeat step 4 through step 12 before
proceeding to step 14.
14. From the Inlet Configuration page, click Events and Triggering, and then click
Next.
15. On the Choose Events page, click Next, leaving the default settings unchanged.
Note: You need not configure event wires.
16. On the Choose Triggering Method page, select Trigger by Contact Closure for the
Pump and Detector, click Next, and then click Finish.

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2.3 Configuring Waters Pump Control software

Note: This procedure is required only in MassLynx software.

To configure Waters Pump Control software:

1. In the Inlet Editor, click Inlet.
2. In the instrument method window, click Config.
3. Enter an appropriate module name for each pump.
Example: In the figure below, pump 1 is named “Alpha Pump” and pump 2
“Beta Pump”.

4. Click Scan.
5. After confirming the presence of all instruments and devices, click Close.

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 6. On the Waters Pump Control Configuration page, under “Associate with an
instrument”, click the list in the box that corresponds to each enabled pump;
then select the appropriate instrument and serial number.

7. Click OK.
8. When prompted, click OK, to confirm and reset communications.

2.4 Configuring the CM-A

You must configure the Column Manager - Active Preheater (CM-A) in the console
software regardless of whether you are using MassLynx or Empower software.

To configure the CM-A:

1. From the Column Manager in the console software, click Configure >
Instrument.
2. In the Instrument Configuration dialog box, from the list on the right-hand side,
select “2 columns”.

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 Requirement: Ensure that the column-position selections on the left-hand
side are specified as shown below.

Restriction: Do not select more than two columns.

3. Click OK, to accept the configuration.
4. From the Column Manager in the console software, select Configure > Operating
Mode.
Result: The Operating Mode Configuration page appears.

5. Click Advanced, select 2 for the left and right positions, select 6 for the left and
right ports, and then click OK.
6. When the Configuration Changed dialog box appears, cycle the power to the
CM-A, and then click OK, to restart and open the console software.
7. Reset Communications in the Inlet Method Editor.
8. In the console software, select Column Manager, and click Configure >
Preheaters.

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 Result: The Preheater Configuration page appears.

Restriction: You can install only one pre-heater in each trough, on either the
left-hand or right-hand side. You select the pre-heater configuration for each
trough on the Preheater Configuration page.
9. Select Active or Passive for column positions 1 and 2.
10. Click OK.
11. When the Configuration Changed dialog box appears, cycle power to the CM-A,
and then click OK, to restart the console software.
See also: ACQUITY UPLC Column Compartments Operator’s Overview and
Maintenance Information for details relating to the CM-A.

2.5 Connecting external valves to the CM-A

Requirement: If you intend to upgrade an existing CM-A as part of a new 2D

system, consult your local service engineer, to ensure compatibility.

To connect an external valve:

1. Attach the adapter cable (part number 441000459) to the connector labeled
COM B on the rear panel of the CM-A.
2. Attach one end of the serial cable supplied with the valve to the adapter cable
and the other end to the valve.
3. Using the address dial on the rear of each valve, set the appropriate address.
Requirement: Each valve must have a unique address setting: 1, 2, or 3, that
corresponds to the external valve identification: 1, 2, or 3.
4. Power-on the CM-A.
5. Power-on the external valves.

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To configure the valves:
1. In the console software, select the Column Manager.
2. Click Configure > Operating System.
3. Mark the check box for the external valve (or valves) that you are using.

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3 System Components and
Features

This chapter gives details of the components ad features found on ACQUITY UPLC with
2D technology instruments.

3.1 Description of ACQUITY UPLC System with 2D

Technology

Figure 3–1: An ACQUITY UPLC system with 2D Technology

Bottle
Tray

Bottle Tray Detector

(optional)

Sample
manager Column
manager
(CM-A)

Solvent Solvent
manager manager

The illustration depicts an ACQUITY UPLC system with 2D Technology. The following
items are required components of the system:

• A column manager (CM-A), with programmable switching valves

• Waters Pump Control software
®
• Windows 7 or Windows XP operating systems. (See the appropriate MassLynx
SCN or Empower release notes for the required workstation configuration.)
• MassLynx version 4.1, with Software Change Note (SCN) 781 (minimum)
• Empower 3, Feature Release 2
• ACQUITY UPLC Systems November 2013 Driver Pack 4

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3.1.1 Column manager (CM-A)
The column manager (CM-A) includes two 6-port, 2-position valves and two column
troughs with independent temperature control. You can use a maximum of two active
pre-heaters when running 2D applications in the CM-A. In the event table, you specify
the valve (Left Valve or Right Valve) and the position of each at a specific time.

Requirement: Confirm that each column is at the desired temperature before

running samples. It is possible to begin an injection before a column reaches its
temperature set point.

Restriction: You cannot use column heaters (CM-Aux) with 2D systems.

Figure 3–2: Column manager, with door open

eCord receptacles
6-port, 2-position valves

Column compartment
trough

Drip tray with leak

sensor
Active preheater retainer
(holds active preheater
TP03446
assembly)

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Figure 3–3: Active preheater assembly

Active preheater
Attached to active retainer
preheater fitting
Active preheater
assembly (fitting
shown below)

From active
preheater

TP03360A

TP03360A
Active preheater fitting

Table 3–1: Column manager components

Component Description
Active Heats the incoming solvent to a specified column temperature
preheater before the solvent enters the column.
assembly
Active Secures the active preheater in the column compartment.
preheater
retainer
Valves 6-port, 2-position valve
Drip tray Captures any leakage from the column or column connections and
routes it to the drip management system.

See also: ACQUITY UPLC Column Compartments Operator’s Overview and

Maintenance Information.

Figure 3–4: Valve (6-port, 2-position)

Hex screw Aligned grooves Cartridge housing

Front view Side view

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 Part number
of CM-A valve Description
cartridge
205000952 Kit, ACQUITY UPLC 2D Technology SS Valve
205000953 Kit, ACQ UPLC 2D Tech. Bio-Inert Valve

Tip: Bio-inert valves are intended for use with highly aqueous, highly ionic, mobile
phases.

3.1.2 Solvent manager

Typical system configurations include two binary solvent managers, or one quaternary
solvent manager and one binary solvent manager. ACQUITY UPLC Systems with 2D
Technology can use as many as three ACQUITY UPLC solvent managers.

Requirement: You must configure the quaternary solvent manager as the

first-dimension pump (Pump 1) in the Waters Pump Control software (see page 17).

Restriction: To avoid problems affecting retention time reproducibility, do not use a

quaternary solvent manager for the second dimension of an analysis.

See also: Your system’s user documentation for additional information on solvent
managers.

3.1.3 Additional pumps

You can use as many as three 515 pumps, or one 1525 pump, in a 2D system. The
additional pumps are for at-column dilution or adding a reagent. The additional
pumps can achieve a flow rate of as many as 10 mL/min, at pressures up to 34,473
kPa (344 bar, 5000 psi). Both the 1525 pump and the pump control module (PCM)
that controls the 515 pumps communicate with the system’s workstation via IEEE
488.

See also:

• Waters 1525μ and 1525EF HPLC Pump Installation and Maintenance Guide
• Waters Pump Control Module II Installation Guide

3.1.4 External valves

The CM-A includes two, 6-port, 2-position valves, and can also independently control
as many as three external valves (listed below) through the analysis method. The
external valves feed via separate power supplies, communicate with the CM-A by
means of an RS232 cable connection (part number 441000459).

Important: If you intend to upgrade an existing CM-A as part of a new 2D system

that includes external valves, consult your local service engineer, to ensure
compatibility.

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 Part number of Pressure
Ports Positions Material
external valve Rating
417000138 10 2 Titanium 34,473 kPa
(344 bar, 5000
psi)
417000139 7 6 PEEK 34,473 kPa
(344 bar, 5000
psi)
417000150 11 10 Stainless Steel 34,473 kPa
(344 bar, 5000
psi)

3.1.5 Tubing
High-pressure, critically clean, tubing assemblies are available for use with 2D
systems. You can choose stainless-steel or MP35N tubing assemblies, depending on
your application. Use MP35N for corrosion resistance in high-ionic strength aqueous
conditions, such as those needed for separating biological samples.

See also:

• Spare parts information on page 39, for more information on tubing assemblies.
• Waters Spare Parts Locator at Waters.com, for a current list of tubing
assemblies.

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September 25, 2015, 715004391 Rev. B
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4 Example System
Configurations

This chapter gives details of system configurations.

4.1 Flow path configurations

ACQUITY UPLC Systems with 2D Technology provide maximum flexibility for

configuring flow paths.

These are some possible flow-path configurations:

• Trap and back-flush elution configuration

• Trap and back-flush elution with at-column dilution (ACD) configuration
• Trap and forward-flush elution configuration
• Heart-cutting configuration
• Parallel column regeneration configuration

Important: In the illustrations depicting each of these configurations, below, Pump

1 is designated the alpha pump and pump 2 the beta pump.

4.1.1 Trap and back-flush elution configuration

Note: During installation, a Waters service engineer performs a system-installation
test for a basic “trap and back-transfer” configuration, to ensure that system modules
perform optimally and that the fluid path is configured correctly.

Trapping occurs when analytes of interest are captured on a trapping column,

constituting the first dimension of the analysis, and then transferred to a second
column, for an analytical separation, the second dimension.

You can use a trap and back-flush elution configuration for various applications
including online sample preparation or simplifying a separation.

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Figure 4–1: Trap and back-flush elution step 1

CM-A

Left Right
W

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

In this configuration, the alpha pump loads the sample onto column 1, trapping
analytes of interest. The alpha pump then performs a wash that retains analytes of
interest and allows some impurities to flow to waste. After the wash, the alpha pump
decreases its flow rate and the left-hand valve in the CM-A switches from position 1 to
position 2.

Figure 4–2: Trap and back-flush elution step 2

CM-A

W Left Right

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

With the left-hand valve in position 2, the beta pump passes a gradient through
column 1, transferring the analytes to column 2 for the analytical separation.

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4.1.2 Trap and back-flush elution with at-column dilution
(ACD)
At-column dilution makes direct injection of a large-volume sample in strong solvent
possible. It does so by diluting the injected sample before it reaches the head of the
column. The following example describes ACD in a trap and back-transfer application.

Figure 4–3: Trap and back-flush elution with at-column dilution step 1

CM-A

Left Right

SM/SM-FTN Mixer Column 1

Column 2

Alpha Beta
BSM/QSM BSM

Line A of the loading alpha pump carries organic solvent and pushes the sample into
the low-volume mixer (part number 205000272). Line B of the alpha pump carries
100% aqueous solution into the low-volume mixer, diluting the sample before it
reaches column 1.

With both the left-hand and right-hand CM-A valve in position 1, the alpha pump
loads the sample onto column 1.

The analytes of interest are then trapped on the head of column 1. The alpha pump
then stops, and the right-hand CM-A valve switches from position 1 to position 2.

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 Figure 4–4: Trap and back-flush elution with at-column dilution step 2

CM-A

Left Right

SM/SM-FTN Mixer Column 1

Column 2

Alpha Beta
BSM/QSM BSM

With the right-hand CM-A valve in position 2, the beta pump starts. A gradient elutes
the analytes of interest from column 1 and onto the more retentive column 2, where
the analytical separation is performed.

4.1.3 Trap and forward-flush elution configuration

The filter-trapping configuration prevents impurities from reaching the head of the
analytical column.

Figure 4–5: Trap and forward-flush elution step 1

CM-A

Left Right
W

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

The sample is injected into the flow produced by the alpha pump. The analytes of
interest pass through column 1, which acts like a filter, trapping impurities. When the
analytes pass through column 1, they are focused onto the head of column 2. The
alpha pump then decreases flow, and the left-hand CM-A valve switches from position
2 to position 1.

September 25, 2015, 715004391 Rev. B

Page 32
 Figure 4–6: Trap and forward-flush elution step 2

CM-A

Left Right
W

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

When the left-hand CM-A valve switches from position 2 to position 1, the beta pump
starts its flow, reconditioning column 1 and sending any impurities to waste. The
alpha pump starts a flow through column 2, to perform the analytical separation.

4.1.4 Valve positions

Figure 4–7: Valve positions

2 1 2 1

3 6 3 6

4 5 4 5

Valve Position 1 Valve Position 2

4.1.4.1 Valve usage

The following usage conditions for the trap and back-transfer configuration are
typical:

• For Pump 1, running water, a flow rate of 1.0 mL/min

• For pump B, running 50:50 acetonitrile/water, a flow rate of 0.7 mL/min
• Column 1 is a 2.1 × 30 mm column, with a particle size of 20 mm
• Column 2 is a 2.1 × 50-mm column, with a particle size of 1.7 mm

September 25, 2015, 715004391 Rev. B

Page 33
 In this configuration, pump 1 (the alpha pump) produces pressures of 6895 kPa
(68.95 bar, 1,000 psi), and pump 2 (the beta pump) produces pressures of 62050
kPa (621 bar, 9,000 psi).

Recommendation: Replace valves after 50,000 cycles of typical use.

4.1.5 Heart-cutting configuration

Heart-cutting is a function whereby analytes of interest that elute from the first
analytical column are then diverted onto a second column, for an additional analytical
separation. Use heart-cutting for obtaining alternate selectivity or for reducing sample
load, for better resolution. The following illustration depicts one method of
heart-cutting.

Figure 4–8: Heart-cutting step 1

CM-A

W
Left Right

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

The alpha pump performs an analytical separation on column 1. A particular

predefined segment of that chromatogram is diverted for a second analytical
separation. As the predefined segment of the analytical separation elutes from
column 1, the left-hand CM-A valve switches from position 1 to position 2.

September 25, 2015, 715004391 Rev. B

Page 34
 Figure 4–9: Heart-cutting step 2

CM-A

W
Left Right

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

With the left-hand CM-A valve in position 2, the beta pump elutes analytes of interest
from column 1 and diverts them to column 2. When the segment of the analytical
separation on column 1 is diverted to column 2, the left-hand CM-A valve switches
from position 1 to position 2.

Figure 4–10: Heart-cutting step 3

CM-A

W
Left Right

SM/SM-FTN Column 1

Column 2

Alpha Beta
BSM/QSM BSM

With the left-hand CM-A valve in position 1, the beta pump performs an analytical
separation of the diverted segment on column 2. The alpha pump re-equilibrates
column 1.

4.1.6 Parallel column regeneration configuration

In parallel column regeneration, one column is used for analysis, while a second
column is re-equilibrated, preparing for the next injection. Over a series of samples
and analytical separations, these columns alternate.

Parallel column regeneration is useful for increasing throughput.

September 25, 2015, 715004391 Rev. B

Page 35
Figure 4–11: Parallel column regeneration step 1

CM-A

Left Right
W

SM Column 1

Column 2

Alpha Beta
BSM BSM

An injection, using flow from the alpha pump, moves the sample onto column 2 for
the analytical separation. The beta pump re-equilibrates column 1, preparing it for the
next injection. After the analytical separation is performed on column 2 and column 1
is re-equilibrated, the left-hand and the right-hand CM-A valves switch from position 1
to position 2.

Figure 4–12: Parallel column regeneration step 2

CM-A

Left Right
W

SM Column 1

Column 2

Alpha Beta
BSM BSM

With both the left-hand and right-hand CM-A valves in position 2, the alpha pump
provides flow for an analytical separation on column 1 and the beta pump
re-equilibrates column 2.

September 25, 2015, 715004391 Rev. B

Page 36
4.2 Comprehensive 2D UPLC

Comprehensive two dimensional (2D) chromatography (C2DC) is a chromatographic

approach whereby two chromatographic systems are connected through the use of
high-pressure switching valves. The two systems (first and second dimensions) are
configured so that effluent from the first dimension is captured and redirected onto
the column of the second dimension. One or more switching valves and sampling
loops are connected so that the flow from the pumps of the respective systems can
alternate between the two sampling loops. Alternation allows for the collection of
effluent from the first dimension into one of the two sampling loops from where it is
diverted into the flow stream of the second dimension by means of a valve switch.
Following the flow’s diversion, the first dimension effluent is collected into the other
sampling loop while the contents of the first loop are processed by the second
dimension. By alternating the flow paths through the two sampling loops, all of the
first-dimension effluent can be, in theory, captured and diverted to the second
dimension, allowing the comprehensive collection and diversion of the entire
first-dimension separation. In essence, C2DC represents automated, on-line, fraction
collection of the effluent from the first dimension separation.

The coupling of two chromatographic systems, each possessing a column, presents

two potential advantages. First, it is a means by which the number of theoretical
plates can be increased for any given sample injection. The second potential
advantage concerning comprehensive 2D chromatography is the opportunity to gain
different or additional information about the sample. To realize this advantage, the
fundamental separation mechanisms of the two dimensions must differ in their
chromatographic conditions. The two dimensions (systems) must represent
orthogonal, or complementary, chromatographic techniques. For example, one
promising combination of C2DC involves partition-based chromatography (usually
reversed phase) in the first dimension and size-exclusion (SEC) or gel-permeation
chromatography (GPC) in the second dimension.

Note: When you configure the system for Comprehensive 2D UPLC using the repeat
feature, select the 2D repeat function, above the Binary Solvent Manager gradient
table and above the Column Manager event table.

Guideline: The time displayed on the last line of both the Binary Solvent Manager
gradient table and the Column Manager event table defines the duration of one cycle.
This duration repeats throughout a sample’s run time.

September 25, 2015, 715004391 Rev. B

Page 37
September 25, 2015, 715004391 Rev. B
Page 38
5 Spare Parts

The table below gives a list of spare parts.

5.1 List of Spare Parts

For information about replacement parts, see the Waters Quality Parts Locator on the
Waters Web site's Services & Support page.

Figure 5–1: Spare parts

Inside
Length inch Fittings (see
Part Number Material Diameter inch
(mm) note, below)
(mm)
430001486 Stainless Steel .010(.254) 24(610) HP/HP
430002582 Stainless Steel .004(.102) 14.5(368) HP/HP
700005476
430002599 Stainless Steel .004(.102) 19.0(483) HP/HP
700005479
430002600 MP35N .004(.102) 14.5(368) HP/HP
430002641 MP35N .004(.102) 19.0(483) HP/HP
430002657 Stainless Steel .004(.102) 22.5(572) LP/LP
700005480
430002658 MP35N .004(.102) 22.5(572) LP/LP
700005485
430002681 Stainless Steel .004(.102) 14.5(368) LP/LP
700005477
430002682 MP35N .004(.102) 14.5(368) LP/LP
700005482
430002683 Stainless Steel .004(.102) 19.0(483) LP/LP
700005478
430002684 MP35N .004(.102) 19.0(483) LP/LP
700005483
430002717 Stainless Steel .004(.102) 22.5(572) HP/HP
430002718 MP35N .004(.102) 22.5(572) HP/HP
430002727 Stainless Steel .010(.254) 30(762) HP/None
430002738 Stainless Steel .007(.178) 19.0(483) HP/HP

September 25, 2015, 715004391 Rev. B

Page 39
Figure 5–1: Spare parts

Inside
Length inch Fittings (see
Part Number Material Diameter inch
(mm) note, below)
(mm)
430002739 Stainless Steel .004(.102) 27.0(686) HP/HP
430002769 Stainless Steel .004(.102) 36.0(914) HP/HP
700005532
430002772 Stainless Steel .004(.102) 44.0(1118) LP/LP
430002779 MP35N .005(.127) 17(432) HP/HP
430002852 Stainless Steel .004(.102) 22.5(572) HP/LP
700005535
430002972 MP35N .004(.102) 6.0(152) HP/HP
430002974 MP35N .004(.102) 27.0(686) HP/HP
430002977 MP35N .004(.102) 36.0(914) HP/HP
430002980 MP35N .004(.102) 44.0(1118) HP/HP
430002983 Stainless Steel .004(.102) 6.0(152) HP/HP
430002986 Stainless Steel .004(.102) 36.0(914) HP/HP
430002992 Stainless Steel .004(.102) 44.0(1118) HP/HP
430002996 Stainless Steel .007(.178) 14.5(368) HP/HP
430002999 Stainless Steel .007(.178) 22.5(572) HP/HP
430003002 Stainless Steel .007(.178) 27.0(686) HP/HP
430003005 Stainless Steel .007(.178) 36.0(914) HP/HP
430003008 Stainless Steel .007(.178) 6.0(152) HP/HP

September 25, 2015, 715004391 Rev. B

Page 40
6 Worksheets

This chapter contains printable, blank worksheets, and examples of completed

worksheets.

6.1 Worksheets

Print the diagrams and tables, below, as needed, to record CM-A setup, valve identity
and valve position. Both blank worksheets and completed example worksheets are
shown.

September 25, 2015, 715004391 Rev. B

Page 41
6.1.1 Blank worksheets
Table 6–1: 2D programing worksheet

Alpha Pump Beta Pump CM-A

Flow Flow
Time %A %B Curve %A %B Curve Valve Position
Rate Rate

September 25, 2015, 715004391 Rev. B

Page 42
 2 1 2 1

3 6 3 6

4 5 4 5

Valve Position 1 Valve Position 2

Time Action Connections and Position

2 1 2 1

3 6 3 6

4 5 4 5

Valve Position _ Valve Position _

C A

D B

2 1 2 1

3 6 3 6

4 5 4 5

Valve Position _ Valve Position _

C A

D B

September 25, 2015, 715004391 Rev. B

Page 43
 2 1 2 1

3 6 3 6

4 5 4 5

Valve Position 1 Valve Position 2

Time Action Connections and Position

2 1

3 6

4 5

Valve Position _

C A

D B

2 1

3 6

4 5

Valve Position _

C A

D B

September 25, 2015, 715004391 Rev. B

Page 44
6.1.2 Completed worksheets
Table 6–2: 2D programing worksheet

Alpha Pump Beta Pump CM-A

Flow Flow
Time %A %B Curve %A %B Curve Valve Position
Rate Rate
0.0 1.0 100 0 0.5 90 10 Left 1

0.5 1.0 100 0 11 0.5 90 10 6 Left 2

0.6 0.1 0 100 11 0.5 90 10 6 Left 2

1.0 0.1 0 100 11 0.5 90 10 6 Left 2

2.0 0.1 0 100 11 0.5 5 95 6 Left 2

3.0 0.1 0 100 11 0.5 5 95 6 Left 2

3.5 1.0 100 0 11 0.5 90 10 6 Left 2

5.5 1.0 100 0 11 0.5 90 10 11 Left 2

September 25, 2015, 715004391 Rev. B

Page 45
Time Action Connections and Position
0.0 to 0.5 Inject sample
Alpha Pump/SM

Waste

Beta Pump

Valve Position 1

Flow

C Trap A

D Analytical B
Detection
Flow

0.0 to 0.5 Trap compound Alpha Pump/SM

Waste

Beta Pump

Valve Position 1

Flow

C Trap A

D Analytical B
Detection
Flow
TP03439

September 25, 2015, 715004391 Rev. B

Page 46
Time Action Connections and Position
0.0 to 0.5 Wash away Alpha Pump/SM
contaminants

Waste

Beta Pump

Valve Position 1

Flow

C Trap A

D Analytical B

Flow Detection

0.5 Switch valve. Alpha Pump/SM

Beta pump now
flows through
the trap column
Waste
in the reverse
direction,
transferring
Beta Pump
analyte to the
head of the
Valve Position 2
analytical
column.
Flow

C Trap A

D Analytical B
Flow Detection
TP03439

September 25, 2015, 715004391 Rev. B

Page 47
Time Action Connections and Position
0.5 to 3.5 Analytical Alpha Pump/SM
separation

Waste

Beta Pump

Valve Position 2

Flow

C Trap A

D Analytical B

Flow Detection

3.5 to 5.5 Switch valve, Alpha Pump/SM

re-equilibrate
system

Waste

Beta Pump

Valve Position 1

Flow

C Trap A

D Analytical B

Flow Detection
TP03439

September 25, 2015, 715004391 Rev. B

Page 48
A Safety Advisories

Waters instruments and devices display hazard symbols that alert you to the hidden
dangers associated with a product’s operation and maintenance. The symbols also
appear in product manuals where they accompany statements describing the hazards
and advising how to avoid them. This appendix presents the safety symbols and
statements that apply to all of Waters’ product offerings.

A.1 Warning symbols

Warning symbols alert you to the risk of death, injury, or seriously adverse
physiological reactions associated with the misuse of an instrument of device. Heed all
warnings when you install, repair, or operate any Waters instrument or device. Waters
accepts no liability in cases of injury or property damage resulting from the failure of
individuals to comply with any safety precaution when installing, repairing, or
operating any of its instruments or devices.

The following symbols warn of risks that can arise when you operate or maintain a
Waters instrument or device or component of an instrument or device. When one of
these symbols appear in a manual’s narrative sections or procedures, an
accompanying statement identifies the applicable risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on an

instrument, consult the instrument’s user documentation for important
safety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

Warning: (Risk of sharp-point puncture injury.)

Warning: (Risk of hand crush injury.)

Warning: (Risk of injury caused by moving machinery.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

September 25, 2015, 715004391 Rev. B

Page 49
 Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

Warning: (Risk of exposure to biological agents that can pose a serious health
threat.)

Warning: (Risk of tipping.)

Warning: (Risk of explosion.)

Warning: (Risk of high-pressure gas release.)

A.1.1 Specific warnings

The following warnings (both symbols and text) can appear in the user manuals of
particular instruments and devices and on labels affixed to them or their component
parts.

A.1.1.1 Burst warning

This warning applies to Waters instruments and devices fitted with nonmetallic tubing.

Warning: To avoid injury from bursting, nonmetallic tubing, heed these

precautions when working in the vicinity of such tubing when it is pressurized:
• Wear eye protection.
• Extinguish all nearby flames.
• Do not use tubing that is, or has been, stressed or kinked.
• Do not expose nonmetallic tubing to compounds with which it is chemically
incompatible: tetrahydrofuran, nitric acid, and sulfuric acid, for example.
• Be aware that some compounds, like methylene chloride and dimethyl
sulfoxide, can cause nonmetallic tubing to swell, significantly reducing the
pressure at which the tubing can rupture.

September 25, 2015, 715004391 Rev. B

Page 50
A.1.1.2 Biohazard warning
The following warning applies to Waters instruments and devices that can process
material containing biohazards, which are substances that contain biological agents
capable of producing harmful effects in humans.

Warning: To avoid infection with potentially infectious, human-sourced

products, inactivated microorganisms, and other biological materials, assume
that all biological fluids that you handle are infectious.
Specific precautions appear in the latest edition of the US National Institutes of
Health (NIH) publication, Biosafety in Microbiological and Biomedical
Laboratories (BMBL).
Observe Good Laboratory Practice (GLP) at all times, particularly when working
with hazardous materials, and consult the biohazard safety representative for
your organization regarding the proper use and handling of infectious
substances.

A.1.1.3 Biohazard and chemical hazard warning

This warning applies to Waters instruments and devices that can process biohazards,
corrosive materials, or toxic materials.

Warning: To avoid personal contamination with biohazards, toxic materials, or

corrosive materials, you must understand the hazards associated with their handling.
Guidelines prescribing the proper use and handling of such materials appear in the
latest edition of the National Research Council's publication, Prudent Practices in the
Laboratory: Handling and Management of Chemical Hazards.
Observe Good Laboratory Practice (GLP) at all times, particularly when working with
hazardous materials, and consult the safety representative for your organization
regarding its protocols for handling such materials.

A.2 Notices

Notice advisories appear where an instrument or device can be subject to use or

misuse that can damage it or compromise a non-clinical sample’s integrity (risks to
clinical sample integrity are accompanied by warning symbols). The exclamation point
symbol and its associated statement alert you to such risk.

Notice: To avoid damaging the instrument’s case, do not clean it with

abrasives or solvents.

September 25, 2015, 715004391 Rev. B

Page 51
A.3 Bottles Prohibited symbol

The Bottles Prohibited symbol alerts you to the risk of equipment damage caused by
solvent spills.

Prohibited: To avoid equipment damage caused by spilled solvent, do not

place reservoir bottles directly atop an instrument or device or on its front
ledge. Instead, place the bottles in the bottle tray, which serves as secondary
containment in the event of spills.

A.4 Required protection

The Use Eye Protection and Wear Protective Gloves symbols alert you to the
requirement for personal protective equipment. Select appropriate protective
equipment according to your organization’s standard operating procedures.

Requirement: Use eye protection when refilling or replacing solvent bottles.

Requirement: Wear clean, chemical-resistant, powder-free gloves when

handling samples.

A.5 Warnings that apply to all Waters instruments

and devices

When operating this device, follow standard quality-control procedures and the
equipment guidelines in this section.

Attention: Changes or modifications to this unit not expressly approved by the

party responsible for compliance could void the user’s authority to operate the
equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément
approuvée par l’autorité responsable de la conformité à la réglementation peut
annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die

ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit
verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des
Systems führen.

Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non

espressamente autorizzata dai responsabili per la conformità fa decadere il
diritto all'utilizzo dell'apparecchiatura da parte dell'utente.

September 25, 2015, 715004391 Rev. B

Page 52
Atencion: cualquier cambio o modificación efectuado en esta unidad que no
haya sido expresamente aprobado por la parte responsable del cumplimiento
puede anular la autorización del usuario para utilizar el equipo.

注意：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設
備的權利。

注意：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操
作该设备的合法性。

주의: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우,

이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.

注意：規制機関か ら 明確な承認 を 受け ず に 本装置の変更や改造 を ⾏ う と 、 本装置の ユ ー

ザ ー と し て の承認が無効 に な る 可能性が あ り ま す。

Warning: Use caution when working with any polymer tubing under pressure:
• Always wear eye protection when near pressurized polymer tubing.
• Extinguish all nearby flames.
• Do not use tubing that has been severely stressed or kinked.
• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated
nitric or sulfuric acids.
• Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic
tubing to swell, which greatly reduces the rupture pressure of the tubing.

Attention: Manipulez les tubes en polymère sous pression avec precaution:

• Portez systématiquement des lunettes de protection lorsque vous vous
trouvez à proximité de tubes en polymère pressurisés.
• Eteignez toute flamme se trouvant à proximité de l’instrument.
• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.
• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou
de l'acide sulfurique ou nitrique concentré.
• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le
gonflement des tuyaux non métalliques, ce qui réduit considérablement leur
pression de rupture.

Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere
Vorsicht angebracht:
• In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille
tragen.
• Alle offenen Flammen in der Nähe löschen.
• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind.
• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder
konzentrierte Salpeter- oder Schwefelsäure verwenden.
Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche
quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert.

September 25, 2015, 715004391 Rev. B

Page 53
Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico
sotto pressione:
• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero
pressurizzati.
• Spegnere tutte le fiamme vive nell'ambiente circostante.
• Non utilizzare tubi eccessivamente logorati o piegati.
• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o
nitrico concentrati.
• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano
rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di
rottura dei tubi stessi.

Advertencia: se recomienda precaución cuando se trabaje con tubos de

polímero sometidos a presión:
• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos
de polímero sometidos a presión.
• Si hubiera alguna llama las proximidades.
• No se debe trabajar con tubos que se hayan doblado o sometido a altas
presiones.
• Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano
(THF) o ácidos nítrico o sulfúrico concentrados.
Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo
dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos.

警告：當在有壓力的情況下使用聚合物管線時，小心注意以下幾點。
• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。
• 熄滅附近所有的火焰。
• 不要使用已經被壓癟或嚴重彎曲管線。
• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。
要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹，大大降低管線的耐壓能力。

警告：当有压力的情况下使用管线时，小心注意以下几点：
• 当接近有压力的聚合物管线时一定要戴防护眼镜。
• 熄灭附近所有的火焰。
• 不要使用已经被压瘪或严重弯曲的管线。
• 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。
要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。

경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.

• 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.
• 근처의 화기를 모두 끄십시오.
• 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.
• 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는
농축 질산 또는 황산과 함께 사용하지 마십시오.
염화 메틸렌 (Methylene chloride) 및 디메틸술폭시드 (Dimethyl sulfoxide) 는
비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오 .

September 25, 2015, 715004391 Rev. B

Page 54
 警告：圧⼒の か か っ た ポ リ マ ー チ ュ ー ブ を 扱 う と き は、 注意 し て く だ さ い。
• 加圧 さ れ た ポ リ マ ー チ ュ ー ブ の付近で は、 必ず保護 メ ガ ネ を 着用 し て く だ さ い。
• 近 く に あ る 火 を 消 し て く だ さ い。
• 著 し く 変形 し た 、 ま た は折れ曲が っ た チ ュ ー ブ は使用 し な い で く だ さ い。
• 非⾦属 チ ュ ー ブ に は、 テ ト ラ ヒ ド ロ フ ラ ン (THF)や⾼濃度の硝酸 ま た は硫酸な ど を 流 さ
な い で く だ さ い。
塩化 メ チ レ ン や ジ メ チ ル ス ル ホ キ シ ド は、 非⾦属 チ ュ ー ブ の膨張 を 引 き 起 こ す場合が あ
り 、 そ の場合、 チ ュ ー ブ は極め て 低い圧⼒で破裂 し ま す。

Warning: The user shall be made aware that if the equipment is used in a
manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une
façon non spécifiée par le fabricant, la protection assurée par le matériel risque
d’être défectueuses.

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei

unsachgemäßer Verwendung des Gerätes die eingebauten
Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: si rende noto all'utente che l'eventuale utilizzo

dell'apparecchiatura secondo modalità non previste dal produttore può
compromettere la protezione offerta dall'apparecchiatura.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma

distinta a la especificada por el fabricante, las medidas de protección del equipo
podrían ser insuficientes.

警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供
的保護將被消弱。

警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供
的保护将被削弱。

경고 : 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단

이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다 .

警告： ユ ー ザ ー は、 製造元 に よ り 指定 さ れ て い な い方法で機器 を 使用す る と 、 機器が提供

し て い る 保証が無効 に な る 可能性が あ る こ と に 注意 し て 下 さ い。

A.6 Warnings that address the replacing of fuses

The following warnings pertain to instruments and devices equipped with

user-replaceable fuses. Information describing fuse types and ratings sometimes, but
not always, appears on the instrument or device.

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Finding fuse types and ratings when that information appears on the
instrument or device

Warning: To protect against fire, replace fuses with those of the type and
rating printed on panels adjacent to instrument fuse covers.

Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles
par d'autres du type et de la puissance indiqués sur le panneau à proximité du
couvercle de la boite à fusible de l'instrument.

Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen
ersetzen, deren Typ und Nennwert auf den Tafeln neben den
Sicherungsabdeckungen des Geräts gedruckt sind.

Attenzione: per garantire protezione contro gli incendi, so8stituire i fusibili

con altri dello stesso tipo aventi le caratteristiche indicate sui pannelli adiacenti
alla copertura fusibili dello strumento.

Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo
y características impresos en los paneles adyacentes a las cubiertas de los
fusibles del instrumento.

警告 ：為了避免火災，更換保險絲時，請使用與儀器保險絲蓋旁面板上所印刷之相同類型與
規格的保險絲。

警告：为了避免火灾，应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险丝。

경고 : 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타입

및 정격의 제품으로 퓨즈를 교체하십시오 .

警告：火災予防の た め に 、 ヒ ュ ー ズ交換で は機器 ヒ ュ ー ズ カ バ ー脇の パ ネ ル に 記載 さ れ て

い る タ イ プ お よ び定格の ヒ ュ ー ズ を ご 使用 く だ さ い。

Finding fuse types and ratings when that information does not appear on the
instrument or device

Warning: To protect against fire, replace fuses with those of the type and
rating indicated in the “Replacing fuses” section of the Maintenance Procedures
chapter.

Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles
par d'autres du type et de la puissance indiqués dans la rubrique
"Remplacement des fusibles" du chapitre traitant des procédures de
maintenance.

Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen
ersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen" des
Kapitels "Wartungsverfahren" angegeben sind.

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 Attenzione: per garantire protezione contro gli incendi, sostituire i fusibili con
altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo
"Sostituzione dei fusibili" del capitolo "Procedure di manutenzione".

Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo
y características indicados en la sección "Sustituir fusibles".

警告 ：為了避免火災，更換保險絲時，應使用 「維護步驟」章節中 「更換保險絲」所指定

之相同類型與規格的保險絲。

警告：为了避免火灾，应更换 “ 维护步骤 ” 一章的 “ 更换保险丝 ” 一节中介绍的相同类

型和规格的保险丝。

경고 : 화재의 위험을 막으려면 유지관리 절차 단원의 “ 퓨즈 교체 ” 절에 설명된 것과 동일

한 타입 및 정격의 제품으로 퓨즈를 교체하십시오 .

警告： 火災予防の た め に 、 ヒ ュ ー ズ交換で は メ ン テ ナ ン ス 項目の 「 ヒ ュ ー ズ の交換」 に 記

載 さ れ て い る タ イ プ お よ び定格の ヒ ュ ー ズ を ご 使用 く だ さ い。

A.7 Electrical and handling symbols

A.7.1 Electrical symbols

The following electrical symbols and their associated statements can appear in
instrument manuals and on an instrument’s front or rear panels.

Symbol Description
Electrical power on

Electrical power off

Standby

Direct current
Alternating current
Alternating current (3 phase)
Safety ground

Frame, or chassis, terminal

Fuse

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 Symbol Description
Functional ground

Input

Output

A.7.2 Handling symbols

The following handling symbols and their associated statements can appear on labels
affixed to the packaging in which instruments, devices, and component parts are
shipped.

Symbol Description
Keep upright!

Keep dry!

Fragile!

Use no hooks!

Upper limit of temperature

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Symbol Description
Lower limit of temperature

Temperature limitation

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