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Biological Sampling

The document discusses biological sampling methods for seawater, phytoplankton, zooplankton, and sediments. It describes using Niskin bottles and a rosette for seawater collection and a CTD. Methods for phytoplankton include settling chambers and preservation in Lugol's iodine or ethanol. Zooplankton are sampled using plankton nets and preserved in formaldehyde. Details are provided on calculating volume filtered and measuring biomass. Sediment sampling methods include grabs and corers.

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Nafisa Anggi
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9 views11 pages

Biological Sampling

The document discusses biological sampling methods for seawater, phytoplankton, zooplankton, and sediments. It describes using Niskin bottles and a rosette for seawater collection and a CTD. Methods for phytoplankton include settling chambers and preservation in Lugol's iodine or ethanol. Zooplankton are sampled using plankton nets and preserved in formaldehyde. Details are provided on calculating volume filtered and measuring biomass. Sediment sampling methods include grabs and corers.

Uploaded by

Nafisa Anggi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Biological Sampling

Dr. Dattesh Desai


CSIR-NIO, Goa
[email protected]
Seawater sampling
Niskin Bottles & Rosette

CTD
Phytoplankton

Settling Chambers

Preservatives: Lugol’s Iodine/Ethanol/ Glutaraldehyde/LN2

Phytoplankton abundance : No./ml or No./l


Zooplankton Sampling
Multiple Plankton Net

Horrizontal Plankton Net Open-Close-Open


System

Flowmeter
Counting Chamber
Based on Bogorov Design
Folsom Plankton Splitter

Zooplankton sample preservative - 4-5% buffered (Sodium Borate) Formaldehyde


(Formalin)
Volume of water filtered

When a circular net is used, the volume of water filtered can be calculated by the
formula given below:

V = (A X R)/K

Where

V = volume of water filtered.


A = mouth area of the net
R = flow meter reading
K = calibration constant of the flow meter
Biomass

Biomass denotes the live weight or the amount of living matter present in the
zooplankton sample.

1. Volumetric (displacement volume and settling volume) method


2. Gravimetric (wet weight, dry weight and ash free dry weight)method
3. Chemical method

Prior to determination of biomass, larger zooplankters such as medusae, ctenophores,


salps, siphonophores and fish larvae should be separated from the zooplankton
sample and their biomass taken separately.
Biomass
Sediment sampling
Grab Corer Deep-Water sediment
collection
Grab Operation
Volume of the Grab

Biomass: gm./m2
Abundance: No./m2

Sieves for benthic studies


Method of collection/Gear Amount of Preservative Type of
sample to be sampling
collected bottle/vial
Microorganisms Water sample/Sediment/ 1ml to 15ml – TBC – Filtered f PVC Vial
zooplankton associated water sample Formaldehyde
Niskin Water Sampler/Grab 5 gm (sediment) /Ethanol/ LN2
Phytoplankton Water sample 500ml – 1000ml Lugol’s PVC Bottle
(Surface/Bottom/Entire water Iodine/Ethanol/ 500/1000ml
column) LN2
Niskin Water Sampler
Zooplankton Net haul (Vertical, Horizontal, 100mt net Formaldehyde/ 1 lit PVC
Oblique)/Use of specialized haul/Pump – 1000 Ethanol bottle
pumps lit water
Benthic – Grab sample/Diving 0.045m2 Formaldehyde/ Dark Bottle
meiofauna Grab/Core Ethanol 250 ml
Benthic – Grab sample/Diving 0.045m2 Formaldehyde/ Plastic
macrofauna Grab/Core Ethanol bags/Bottle

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