NECROSIS || **COAG** (Archi preserved; MC; H Sp Kid, TOMBSTONE) **LIQ**

(Digestion of tissue, BR SC) **CAS** (Combo of coag/liq; cheesy masses) **GANG** (int.
blood supp; wet - venous occlusion, dry - arterial) **FAT** (enz. digestion) **FIBoid**
(deposition of fib-like CHON in arterial wall) || SEC. CHANGES || (Pallor, Algor - 7*C/hr,
Rigor, Livor mortis, Putref, PstM clotting - currant jelly, Dessic, Autolysis, Sk'tn.) || Livor Mort
(Ooze upon incs'n), Ecchy (prom when pressure is app and disappears, no oozing) || PstM (settle
of RBC from plasma) (Chicken fat) (Currant) (Assumes vessel shape) (Rubbery const) AntM
(granular) (friable)

AUTOPSY TECH || (Virchow - organ by organ) (Rokltansky - in-situ exam, notable organs)
(Ghon - TIABN, En Block) (Lutelle - En Masse, all viscera) || MACROPHAGES || (BL - Mono)
(BR - Microglia) (BN - Osteoclast) (BM - Promono) (Skin - Langerhan) (Conn T - Histiocyte)
(Lungs - Dust) (Liver - Kupffer) (Spleen - Littoral) (Kidney - Mesangial) (Placenta - Hofbauer)
(Lymph - Dendritic)

[ 4 ] RT Specimens || **SP** (most common) (Saccomano's fix, 3 sp and 2-3 slides per sp) **Br
Brush** (Pull apart) **BAL** (Detect P. jiro) **Serous Fluids** (300u heparin/100 mL)
**Breast smear** (Any) || UT Specimens || **Voided UR** (male) **Cath. UR** (female)
**Washings** (both) || BODY CAVITY (Exu/Trans) || GIT Sp (<30 mins processing) || FIXES ||
**EtOhEth** (best but flammable) **Saccomano** (50 EtOH, 2 carbw) **95 EtOH** (UR,
Brc, Gst) **Carnoy's** (fastest) **50 R-OH** (for all types of effusion) **70 R-OH** (SP
only)

MAILING || (Air dry after 2 hr fix then place in wood, cardboard, plastic) (Glycerin tech - fix for
30 mins, cover with gly, then coverslip) || ADHESIVES || (Pooled ser/plasma) (Celloidin ether
alc) (Leuconostoc) || REQ ADHESIVE || (Conc. SP) (UR Sediment) (BAL) (Gastric lav) ||
GYNE STUDS || (Upper lat 3rd - hormonal) (Ecto - SEC) (Endo - lined by SiCol) (T Zne - cerv
C) (Vaginal scrape - hysterectomy) (4 quad - localize adenosis) (Vulvular - evaluate herpetic
lesions)

CVD Cells || ***MatSuf C*** (45 - 50 um) (Pale, Pk cyto, D Pyknotic nuc <6um) [Tr Acd - E2
influence; Pseudo - drying] ***Int C*** (mid, polyhedral, elongated w basophilic vacuolated
cyto) [Navi - boat; curls on edge; E2 Prog Effect; latter half of menses, preg, mp] [Preg C -
round/oval boat with trans baso cyto central, DB in periphery] ***Pbasal*** (fried, sunny side
up) (15-30 um) (2wk, childbirth, abort, mp) || ORGANS THAT DO NOT SHED
SPONTANEOUSLY (Breast, Thy, LN, Liv, Lung, Skin, Soft T, Bones)
OTHER ELEMENTS || ***Edmet C*** ( >g3) (during and 1-10 post mens) (ovarian hormone
shred) ***Ecerv GC*** (large g/small sheets) (cyto pale b/gy, fine vac, indc borders,
honeycomb) ***Basal C*** small, round, large nuc >half vol (pub, post mp) ***L.
acidophilus*** (MC vag flora, stubby baci, 3.5-4.5 pH) (B, Lv in pap) ***Lepto*** (long, thin,
filamentous, hair-like, inc vag pH) ***RBC*** trauma colxn, mens ***NEUTS*** (inc post
mens) (assoc infxn) ***TALC*** (comm. contam) ***C. Alb*** (shish kebab) ***T. vagi***
BGy to BG blob (Ping-pong Dse, STD) ***Clue Cell*** (abn var SEC, Moth-eaten) (G. vagi)
(Non-specific bact vaginosis) ***Koilocytes*** (HPV 18/16, wrinkled prune, perinuc halo,
LSIL ***HSV II*** (macrosomia, multinuc, nuc mold, ground glass chrm) ***FERNING***
(arborization, muc under E2 inf, early detection of preg)

RECORDS || **I, QC, R** (2 years) **BB QC** (5) **BB sig** (10) **BB record** (Indef.) ||
REPORTS || **CP** (2) **SP** (10) **Cytogen** (20) **Autopsy forensic** (Indef.) ||
SPECIMENS || **Serum, BF, OBF** (48 hrs) **BL Smear, Micro Smear, BB spx** (7 days)
**Cytogen** (3 years) **Patho block, slides** (10 years) **Cytogen images** (20 years) || (2)
FORENSIC CASES || **BF, Toxo** (1 year) **Wet Tissue** (3 years) **Para blocks, Slides,
Reps, Gross photo/neg, Dried BL films, Frozen tissue for DNA (INDEFINITE) || RELEASING ||
**SP/CP** <24 hrs **FrS** 5-15 mins **Autopsy** < 1 week

MICROANATOMICAL FIX || (10 NBF) (10 FormolSal) (Heiden) (Formol sub) (Zenker)
(ZenFor-Helly) (Brasil) (Bouin) || NUC || (BeNCH Flix) || CYTO || (RHeFoch FlOur) || Histo ||
FoS-A-A(lc)N || POST CHROM: 2.5-5% PotDi (mordant) 24 hrs. || IMPROPER FIXATION ||
(Fail to arrest autolysis - fail to fix immediately) (Removal of subs solube to fix - wrong choice)
(Art pigments - Inc washing) (Soft/Feather-like - Inc fix) (Loss/inactivation enz -wrong choice)
(SHrink/Swlling - overfix) (Brittle and hard - prolonged)

ALD FIX || **Form** MC (work: 10) (stock: 37-40) (24 hr) *pigments* (paraf: white crys ppt -
prol. storage, rev. 10% MetOH, filt) (acid f hematin: BlkBr deposits - seen in microscope, rev.
via Kard, Lillies, Picrate, Alc. KOH) **10NBF** (best general fix) **10ForSal** (CNS and
gen. post mortel, lipids) **ForSubl** (routine post mortem) **ForCal** (Lipids) **Paraf**
(ImmCyChem) **Glut** (2.5% - small t, 2-4 hrs) (4% - large, 6-24) **Karnovsky PFG** (resin
and ehc)