Certified Reference

Materials
for
UV, Visible, NIR and IR
Molecular Spectroscopy

RM-1N2N3NHGDG

Set Serial No: 43162

Customer Details:
Starna GmbH
Schillerstrasse 12
D-64319 Pfungstadt
GERMANY

The customer information stated on this page

number 1, applies to all certificates.

Original Starna references are manufactured under ISO 17034:2016 accreditation

All calibration measurements are performed under ISO/IEC 17025:2017 accreditation.

 Reference Material
Certificate of Calibration and Traceability
Calibration Lab. Starna glass filters for use in the Visible region of the spectrum to
Starna Scientific Ltd verify the transmission and absorbance scales.
52/54 Fowler Rd
HAINAULT Starna Neutral Density Filter Set
Essex IG6 3UT Certificate Number: 118401
England Certificate Date: 22 January 2024
22 January 2026
0659
Tel. +44 (0)20 8501 5550 Expiration Date:
Analysis file number: 16955
Page Number 2 of 3
Set Serial Number: 43162
Email: [email protected] Blank Serial Number: 128544

Description of Reference Material:

The reference filters are prepared from one of the following Schott optically neutral glass NG-3, NG-4, NG-5 or NG-11 depending
on the nominal transmission required for each individual filter. Each filter is mounted in a black-anodized aluminium alloy holder,
complete with front and rear slide cover.
All procedures are implemented in accordance with ISO/IEC 17025 and ISO 17034. Additional information can be found on the
Starna web site at www.starna.com

Certified Values of Reference Material:

Each individual filter is measured against the empty filter holder (blank). The net absorbance and transmision values are measured
at the wavelengths specified in the table below. The results and associated uncertaintiesat a coverage probability of 95 % as detailed
below were obtained using the analytical procedures detailed in NIST Special Publication 260-140.

The combined analytical and instrument uncertainties at a coverage probability of 95 % is:

For Absorbance values less than 1.2 A (greater than 6.3 T/%) the expanded uncertainty = +/- 0.0027 A
For Absorbance value in the range 1.2 to 1.9 A (6.3 - 1.6 T/%) the expanded uncertainty = +/- 0.0052 A
For Absorbance value in the range 1.9 to 2.2 A (1.6 - 0.6 T/%) the expanded uncertainty = +/- 0.0059 A
For Absorbance value in the range 2.2 to 2.7 A (0.6 - 0.2 T/%) the expanded uncertainty = +/- 0.011 A
For Absorbance values greater than 2.7 A (Less than 0.2 T/%) the expanded uncertainty = +/- 0.019 A

The reported expanded uncertainty is based on a standard uncertainty multiplied by a coverage factor k=2. providing a coverage
probability of approximately 95%. The uncertainty evaluation has been carried out in accordance with UKAS requirements.
(note net absorbance and transmission values can be measured at additional wavelengths if requested .)

Serial # Wavelength (nm) 440.0 465.0 546.1 590.0 635.0

131135 Absorbance 1.0032 0.9460 1.0013 1.1131 1.0987

%T 9.93 11.32 9.97 7.71 7.97

125468 Absorbance 0.7020 0.6629 0.6990 0.7744 0.7638

%T 19.86 21.73 20.00 16.81 17.23

127715 Absorbance 0.5569 0.5193 0.5203 0.5541 0.5616

%T 27.74 30.25 30.18 27.92 27.44

This certificate is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to the SI system of units and/or to units of measurement realised at NIST, NPL or other recognised National Metrology Laboratorie. This certificate may not
be reproduced other than in full, except with the prior written approval of the issuing laboratory.
Set Serial Number: 43162
Page Number 3 of 3
Certificate Number: 118401
Certificate Date: 22 January 2024
UKAS Accredited Calibration Laboratory No. 0659
Analysis Date: 19 January 2024
Analysis file number: 16955

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of high performance This certificate is valid for a maximum period of two years from the date
reference spectrophotometers. The instruments are tested and qualified to of issue or sooner if specified by the user's own protocols. Although the
the manufacturer's published specification over the analytical range used references are covered by a lifetime guarantee this is subject to certain
for the reference material certification. conditions, see guidance notes.
The following primary references and fundamental procedures are used in
the qualification of the reference spectrophotometers:
Re-certification Procedure:

Absorbance: NIST SRM 2031, 1930 & 930e, Double aperture method All reference materials are certified and supplied in a useable condition.
Wavelength: NIST SRM 2034, Emission lines of Hg & deuterium
There is no warranty for fitness beyond receipt by the customer. When
references need to be re-certified or inspected for any reason, customers
Stray Light: NIST SRM 2032, KCl, KI & lithium carbonate
should return them to the Starna ISO/IEC 17025 & ISO 17034 accredited
Resolution: Benzene vapor, half width of D2 656.1 nm line
calibration laboratory, where all original data is collated.

Calibration Method: On receipt by Starna Scientific the references are measured “As received”,
before cleaning under the re-certification procedure. “As received” data is
The conditions of analysis used to generate the certified values on this available on request.
certificate are as listed in the chart below:

Reference Materials:
NG-3, NG-4, NG-5 & NG-11 Optically Neutral Glass Storage and Care:
Reference: Empty filter holder (air)
References should always be stored in the box provided and handled with
Scale: Transmission & Absorbance
extreme care. Filters are fragile and should be inserted and removed from
Range: See Table on page 2. the instrument taking care not to twist or apply leverage against the cell
Band width: 1 nm +/- 0.2nm holder, as this may crack the filter. Damage in the form of scratches or
Temperature: 22 +/- 1.0 °C contamination may alter the certified values significantly such that they
need re-certifying or even complete replacement. For cleaning see
Instructions for Use: guidance notes.

Remove the sliding window covers from both sides of each filter to be
used. Place the empty (blank) filter holder in the blank cell holder in your Instrument Dependencies:
instrument. Place the filters in the sample compartment as you would for The instrument to be checked should have a spectral bandpass not
any sample. Determine the absorbance of each filter against the supplied exceeding 6.5 nm. Consult your instrument owners manual for this
blank at each of the five listed wavelengths. Repeat several times. information.

To assess photometric accuracy, compare the mean, net absorbance or Calibration performed by:
transmission reading for each filter and wavelength to the published values
on this certificate. The absolute difference between the mean measured
value and the certified value will not exceed the sum of the certified Calibration Technician - P. Wakelin RSci MRSC MIScT
uncertainty and the specified accuracy of the instrument if the instrument is Approved Signatory:
performing correctly.

Calibration Manager - A. Wakelin CSci CChem MRSC

All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing laboratory
This certification must not be used by a third party to claim product endorsement by the accreditation body or any agency of the U.S. or
U.K. governments.

This certificate is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to the SI system of units and/or to units of measurement realised at NIST, NPL or other recognised National Metrology Laboratorie. This certificate may not
be reproduced other than in full, except with the prior written approval of the issuing laboratory.
 Reference Material
Certificate of Calibration and Traceability
Calibration Lab. Holmium glass filter for use as a wavelength accuracy reference in the
Starna Scientific Ltd UV and visible spectrum
52/54 Fowler Rd
HAINAULT Certificate Number: 118402
Essex IG6 3UT Certificate Date: 22 January 2024 0659
England Expiration Date: 22 January 2026
Tel. +44 (0) 20 8501 5550 Analysis Number: TBM140322 Page Number 2 of 3

Set Serial Number: 43162

Email: [email protected] Cell Serial Number: 129172

Description of Reference Material:

This reference material consists of an optical glass filter containing holmium oxide which has distinct absorption bands. The reference material is
designed for the verification and calibration of the wavelength scales of visible and ultraviolet spectrophotometers having nominal spectral bandwidths of
5 nm or less.
At higher spectral bandwidths ( > 3.0 nm ) peaks identified with '.00' will not be listed. as they cannot be resolved by the instrument.
All procedures are implemented in accordance with ISO/IEC 17025 and ISO 17034. Additional information can be found on the Starna web site at
www.starna.com

Certified Values of Reference Material:

The holmium glass filter is measured in the absorbance mode against an air blank, over the wavelength range of 642 to 218 nm. For each spectral
bandwidth, a baseline correction is performed with an empty cell holder.
The 14 peak maxima are identified and certified to be within the expected wavelength range tolerance for each spectral bandwidth (SBW).

The combined analytical and instrument uncertainties at the 95% confidence level is 0.11 nm.

The reported expanded uncertainty is based on a standard uncertainty multiplied by a coverage factor k=2. providing a coverage probability of
approximately 95%. The uncertainty evaluation has been carried out in accordance with UKAS requirements

SBW Wavelengths in nanometers of peak maxima as referenced to air, +/- 0.11 nm

0.10 641.62 537.20 472.77 457.65 452.76 447.48 417.52 360.15 333.72 287.25 278.75 260.47 241.32 219.68

0.25 641.54 537.11 472.74 457.66 452.80 447.50 417.56 360.15 333.72 287.25 278.73 260.52 241.32 219.68

0.50 641.47 537.17 472.74 457.62 452.77 447.48 417.54 360.19 333.76 287.30 278.72 260.56 241.35 219.72

1.00 641.53 537.18 472.76 457.58 452.73 447.53 417.58 360.29 333.74 287.40 278.70 260.73 241.45 219.72

1.50 641.58 537.18 472.75 457.50 452.68 447.58 417.59 360.45 333.77 287.56 278.70 260.97 241.55 219.73

2.00 641.60 537.22 472.70 457.25 452.56 447.64 417.60 360.60 333.79 287.79 278.68 261.00 241.55 219.63

3.00 641.65 537.32 472.59 .00 452.02 448.04 417.60 360.85 333.79 288.00 278.61 261.02 241.47 219.47

4.00 641.76 537.49 472.19 .00 .00 448.99 417.67 360.95 333.72 288.06 278.50 260.89 241.31 .00

5.00 641.95 537.75 471.51 .00 .00 449.86 417.80 361.02 333.67 288.07 278.25 260.57 241.01 .00

This certificate is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to the SI system of units and/or to units of measurement realised at NIST, NPL or other recognised National Metrology Laboratorie. This certificate may not
be reproduced other than in full, except with the prior written approval of the issuing laboratory.
Starna Cell Serial Number: 129172 Page Number 3 of 3
Certificate Number: 118402
Certificate Date: 22 January 2024 UKAS Accredited Calibration Laboratory No. 0659
Verification Date: 22 January 2024

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of high performance This certificate is valid for a maximum period of two years from the date
reference spectrophotometers. The instruments are tested and qualified to of issue or sooner if specified by the user's own protocols. Although the
the manufacturer's published specification over the analytical range used references are covered by a lifetime guarantee this is subject to certain
for the reference material certification. conditions, see guidance notes.
The following primary references and fundamental procedures are used in
the qualification of the reference spectrophotometers:
Re-certification Procedure:
Absorbance: NIST SRM 2031, 1930 & 930e, Double aperture method
Wavelength: NIST SRM 2034, Emission lines of Hg & deuterium All reference materials are certified and supplied in a useable condition.
Stray Light: NIST SRM 2032, KCl, KI & lithium carbonate There is no warranty for fitness beyond receipt by the customer. When
Resolution: Benzene vapor, half width of D2 656.1 nm line references need to be re-certified or inspected for any reason, customers
should return them to the Starna ISO/IEC 17025 & ISO 17034 accredited
Calibration Method: calibration laboratory, where all original data is collated.

The conditions of analysis used to generate the certified values on this

On receipt by Starna Scientific the references are measured “As received”,
certificate are as listed in the chart below: before cleaning under the re-certification procedure. “As received” data is
Filter Material Holmium oxide glass available on request.
Reference: Air
Scale: Absorbance
Range: 642 to 218 nm Storage and Care:
Band width: Multiple References should always be stored in the box provided and handled with
Temperature: 23.5° C +/- 1.0 °C extreme care. Filters are fragile and should be inserted and removed from
the instrument taking care not to twist or apply leverage against the cell
Instructions for Use: holder, as this may crack the filter. Damage in the form of scratches or
contamination may alter the certified values significantly such that they
Remove the sliding window covers from both sides of each filter to be
need re-certifying or even complete replacement. For cleaning see
used. Place the filter in the sample compartment as you would for any
guidance notes.
sample. Leave the reference cell holder empty as all measurements are to
be made against air. Measurements should be made within the temperature
range of 20° to 30° C. In the absorbance mode scan the filter over the
required range. Find the absorbance maxima and compare them to the
certified wavelengths on this certificate as indicated for the spectral Calibration performed by:
bandwidth (SBW) used by your instrument. If you find any significant
differences, it is recommended that a service technician inspect your
instrument to determine the source of the discrepancy. Calibration Technician - P. Wakelin RSci MRSC MIScT
Approved Signatory:
Instrument Dependencies:
The instrument to be tested should have a SBW not exceeding 5 nm.
Consult the instrument owners handbook for this information. Calibration Manager - A. Wakelin CSci CChem MRSC

All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing laboratory This certification must not be
used by a third party to claim product endorsement by the accreditation body or any agency of the U.S. or U.K. governments.

This certificate is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to the SI system of units and/or to units of measurement realised at NIST, NPL or other recognised National Metrology Laboratorie. This certificate may not
be reproduced other than in full, except with the prior written approval of the issuing laboratory.
 Reference Material
Certificate of Calibration and Traceability
Calibration Lab. Didymium glass filter for use as a wavelength accuracy reference in the UV
Starna Scientific Ltd and visible spectrum
52/54 Fowler Rd
HAINAULT
Certificate Number: 118403
Essex IG6 3UT
Certificate Date: 22 January 2024 0659
England
Expiration Date: 22 January 2026
Tel. +44 (0) 20 8501 5550 Page Number 2 of 3
Analysis Number: DG03060801
Set Serial Number: 43162
Email: [email protected] Cell Serial Number: 125234

Description of Reference Material:

This reference material consists of an optical glass filter containing didymium (a mixture of neodymium and praesodymium) which
has distinct absorption bands. The reference material is designed for the verification and calibration of the wavelength scales of
visible and ultraviolet spectrophotometers having nominal spectral bandwidths of 5 nm or less. All procedures are implemented in
accordance with ISO/IEC 17025 and ISO 17034. Additional information can be found on the Starna web site at www.starna.com

Certified Values of Reference Material:

The didymium glass filter is measured in the absorbance mode against an air blank, over the wavelength range of 890 to 430 nm.
For each spectral bandwidth, a baseline correction is performed with an empty cell holder.
The 11 peak maxima are identified and certified to be within the expected wavelength range tolerance for each spectral bandwidth
(SBW).

The combined analytical and instrument uncertainties at a coverage probability of 95 % is 0.11 nm.

The reported expanded uncertainty is based on a standard uncertainty multiplied by a coverage factor k=2. providing a coverage
probability of approximately 95%. The uncertainty evaluation has been carried out in accordance with UKAS requirements

SBW Wavelengths in nanometers of peak maxima as referenced to air, +/- 0.11 nm

0.10 879.22 806.96 748.56 741.21 684.52 585.25 572.89 528.89 513.44 472.75 431.04

0.25 879.44 807.07 748.49 741.22 684.51 585.15 573.01 528.89 513.42 472.71 431.07

0.50 879.45 807.10 748.58 741.04 684.48 585.27 572.97 528.89 513.41 472.61 431.12

1.00 879.41 807.02 748.56 741.02 684.50 585.19 572.99 528.90 513.39 472.66 431.22

1.50 879.28 807.03 748.55 740.72 684.40 585.35 573.17 528.88 513.47 472.50 431.38

2.00 879.27 807.03 748.48 740.18 684.49 585.29 573.33 528.83 513.41 472.47 431.57

3.00 879.40 807.02 748.37 740.15 684.31 585.43 573.93 528.69 513.23 472.16 431.90

4.00 879.70 807.16 748.28 740.27 684.63 585.48 574.60 528.72 513.70 472.22 440.74

5.00 879.68 807.04 747.61 740.51 684.70 585.56 575.10 528.59 513.75 479.88 441.29

This certificate is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to the SI system of units and/or to units of measurement realised at NIST, NPL or other recognised National Metrology Laboratorie. This certificate may not
be reproduced other than in full, except with the prior written approval of the issuing laboratory.
Starna Cell Serial Number: 125234 Page Number 3 of 3
Certificate Number: 118403
Certificate Date: 22 January 2024 UKAS Accredited Calibration Laboratory No. 0659
Verification Date: 22 January 2024

Certifying Instrument Qualification: Duration of Certificate:

All calibration is performed on one of a series of high performance This certificate is valid for a maximum period of two years from the date
reference spectrophotometers. The instruments are tested and qualified to of issue or sooner if specified by the user's own protocols. Although the
the manufacturer's published specification over the analytical range used references are covered by a lifetime guarantee this is subject to certain
for the reference material certification. conditions, see guidance notes.
The following primary references and fundamental procedures are used in
the qualification of the reference spectrophotometers:
Re-certification Procedure:
All reference materials are certified and supplied in a useable condition.
Absorbance: NIST SRM 2031,1930 & 930e, Double aperture method
There is no warranty for fitness beyond receipt by the customer. When
Wavelength: NIST SRM 2034, Emission lines of Hg & deuterium
references need to be re-certified or inspected for any reason, customers
Stray Light: NIST SRM 2032, KCl, KI & lithium carbonate
should return them to the Starna ISO/IEC 17025 & ISO 17034
Resolution: Benzene vapor, half width of D2 656.1 nm line
accredited calibration laboratory, where all original data is collated.

Calibration Method: On receipt by Starna Scientific the references are measured “As
The conditions of analysis used to generate the certified values on this received”, before cleaning under the re-certification procedure. “As
certificate are as listed in the chart below: received” data is available on request.

Filter Material Didymium oxide glass

Reference: Air
Scale: Absorbance Storage and Care:
Range: 890 to 430 nm
Band width: Multiple References should always be stored in the box provided and handled with
Temperature: 23.5° C +/- 1.0 °C extreme care. Filters are fragile and should be inserted and removed from
the instrument taking care not to twist or apply leverage against the cell
Instructions for Use: holder, as this may crack the filter. Damage in the form of scratches or
contamination may alter the certified values significantly such that they
Remove the sliding window covers from both sides of each filter to be need re-certifying or even complete replacement. For cleaning see
used. Place the filter in the sample compartment as you would for any guidance notes.
sample. Leave the reference cell holder empty as all measurements are to
be made against air. Measurements should be made within the temperature
range of 20° to 30° C. In the absorbance mode scan the filter over the
required range. Find the peak maxima and compare them to the certified
wavelengths on this certificate as indicated for the spectral bandwidth Calibration performed by:
(SBW) used by your instrument. If you find any significant differences, it
is recommended that a service technician inspect your instrument to
determine the source of the difference.
Calibration Technician - P. Wakelin RSci MRSC MIScT
Approved Signatory:
Instrument Dependencies:
The instrument to be tested should have a SBW not exceeding 5 nm.
Consult the instrument owners handbook for this information.
Calibration Manager - A. Wakelin CSci CChem MRSC

All rights reserved. This certificate may not be reproduced other than in full, except with the prior written approval of the issuing laboratory This certification must not be used by
a third party to claim product endorsement by the accreditation body or any agency of the U.S. or U.K. governments.

This certificate is issued in accordance with the laboratory accreditation requirements of the United Kingdom Accreditation Service. It provides traceability of
measurement to the SI system of units and/or to units of measurement realised at NIST, NPL or other recognised National Metrology Laboratorie. This certificate may not
be reproduced other than in full, except with the prior written approval of the issuing laboratory.