3d Printing - Axo1 Supplementry
3d Printing - Axo1 Supplementry
Review of Low-Cost 3D Bioprinters: State of The Market and Observed Future Trends
Anh Tonga, Quang Long Phama, Paul Abatemarcoa, Austin Mathewb, Dhruv Guptaa, Siddharth
a
The Otto H. York Department of Chemical and Materials Engineering, New Jersey Institute of
b
Department of Biomedical Engineering, New Jersey Institute of Technology Newark College
†
Corresponding author:
Prof. Roman Voronov, The Otto H. York Department of Chemical and Materials Engineering,
New Jersey Institute of Technology Newark College of Engineering, 161 Warren Street,
Table S1. Technical feature comparison of the low-cost bioprinters, clustered by the manufacturer. Note: RT = Room Temperature.
Color-code: ✓ = Microextrusion-based Bioprinters; = Droplet-based Bioprinters; = Light-based Bioprinters.
XYZ
WxDxH XYZ Build On- No. of
Positional Sterile Print Bed Print Head Inspection Control
Company Model Name Dimension Volume Enclosure* Board Printing Technology Print Photocuring Calibration
Accuracy Environment Temp. Control Temp. Control Camera Software
(cm) (cm) Display Heads
(µm)
Modular heads:
Interactive –
29 x 19 x Yes – Mechanical, Precision:
3D Cultures Tissue Scribe 10 x 12 x 8 Open 1 No No RT – 60 °C RT – 70 °C No Open Source Adjustable
36 Non touch Thermoplastic 100 x 100 x 44
Nozzle Height
extrusions
Pneumatic extrusion
10 x 10 x 10
(0.5-85 Psi)
Advanced BIOBOT™ 30 x 52 x Rotary ɸ 19 x & Included –
Open No (Turret dispensing print 5 No No No No No Automated
Solutions, Inc. BASIC 55 10 height Repeatability: TSIM® (1 year)
head with revolving
10 x 10 x 10
motion)
Optional
Yes
Mechanical
Modular heads: Optional 50 x 50 x 10 &
Yes – extruder:
33 x 54 x Mechanical, 365 / 405 / & Optional Optional Included –
Engine SR 20 x 20 x 20 Open Tablet 3 No 0 °C – 270 °C; Automated
56 Thermoplastic, Electro- 450 nm tool Repeatability: RT – 200 °C 200x USB Gib System
included Thermoplastic
melt printing extrusions head 25 x 25 x 5 Microscope
extruder: 150 °C
tool head
– 450 °C
Hyrel3D
Optional
Yes
Mechanical
Modular heads: Optional 12 x 12 x 10 &
Yes – extruder:
33 x 48 x Mechanical, 365 / 405 / & Optional Optional Included –
Engine HR 10 x 10 x 10 Open Tablet 4 No 0 °C – 270 °C; Automated
51 Thermoplastic, Electro- 450 nm tool Repeatability: RT – 200 °C 200x USB Gib System
included Thermoplastic
melt printing extrusions head 12 x 12 x 5 Microscope
extruder:
tool head
150 °C – 450 °C
405 nm Extruder 1:
Pneumatic extrusion (3- Included –
31 x 31 x / Precision: RT – 160 °C;
Allevi Allevi 2 9x9x9 Open No 120 psi with ±1 psi 2 No No No Bioprint Automated
31 365 nm 5x5x1 Extruder 2:
variance) Essential
optional RT – 70 °C
Optional
nScripp
Pneumatic
Modular heads: extruder:
Optional
Pneumatic, Thermoplastic -10 °C – 70 °C Yes
137 x 84 x 22.2 x 15.2 x 10 °C – 50 °C
BAT Closed No Extrusions, Pick-n-place 5 365,385 & 405 nm 10 x 10 x 5 Optional or & Automated
196 15.2 or
for electronic circuits, 25 °C – 90 °C; Customizable
25 °C – 150 °C
nMill Thermoplastic
extruder:
25 °C – 450 °C;
5 x 5 vacuum
chuck
Aspect 56 x 47 x 1 for up to
RX1 Or Open No Microfluidic Extrusion N/A 0.5 x 0.5 x 0.5 No N/A N/A Yes Automated
Biosystem 47 6 fluids
bigger substrate
optional
Yes –
Compatible: Excitation
Yes Microfluidic 1 for up to Inverted
70 x 57 x 3.5/5 cm Petri- objective: 370 –
Fluicell AB Biopixlar® Closed & hydrodynamic confined three 2x2x2 No No Optional Microscope for Automated
80 dish; 6-well 410 nm; 473 – 491
gamepad flow – with Robot Arm materials fluorescence &
plates nm; 580 – 598 nm
bright-field
Modular heads: Integrated
135 × 89 × 6-well & 12- Integrated Yes –
Poietis NGB-R Closed Pneumatic extrusion & 3 365 & 405 nm 10 x 10 x 10 Optional 30 – 50 °C microscope Automated
233 well plates PC Class II BSC
Inkjet with robot arm optional
1.3 x 1.3 x 1.3
hold up to 3
PC & No –
DigiLab CellJet N/A standard 96 Open Inkjet – Solenoid Valve 8 N/A No No No Automatic
included Repeatability: But has plate cover
well plates)
10 x 10 x 10
Yes –
Dual HEPA Filter –
48 x 56 x Standard multi- Auto cleaning
Inventia Rastrum Closed No Inkjet – Solenoid Valve 8 N/A 20 x 20 x 20 No No No Automatic
49 well plates function with
ethanol & Milli-Q
water
20 x 20 x 20 Optional
Yes –
Footprint: Build area: PC Modular heads: & Optional Heated vacuum Optional
jetlab® 4xl-A Closed 4 No Horizontal & Automated
63 x 57 21 x 26 included Inkjet – Piezo DOD Repeatability: HEPA filter platen: -195 °C – 300 °C
vertical cameras
5x5x5 RT – 100 °C
20 x 20 x 20 Optional Automated
Yes –
Footprint: Build area: PC Modular heads: & Optional Heated vacuum Optional &
jetlab® 4xl-B Closed 4 No Horizontal &
63 x 57 21 x 26 included Inkjet – Piezo DOD Repeatability: HEPA filter platen: -195 °C – 300 °C Integrated ultra
vertical cameras
5x5x5 RT – 100 °C balance
MicroFab
15 x 15 x 15
or
Footprint: Build area: 4x4x4 Optional Automated
Yes –
112 x 71 30 x 30 PC Modular heads: & Optional Heated vacuum Optional &
jetlab® II Closed 4 No Horizontal &
or or included Inkjet – Piezo DOD Repeatability: HEPA filter platen: -195 °C – 300 °C Integrated ultra
vertical cameras
120 x 75 40 x 40 5x5x5 RT – 100 °C balance
or
2x2x2
Autodrop
Pipettes: No;
25 x 25 x 25
Modular heads: Micro dispenser:
Autodrop Compact 56 x 55 x PC &
21 x 21 x 11 Closed Inkjet – Piezo DOD, 2 365-405 nm No No nozzle Yes Automatic
– AD-P-7000 77 included Repeatability:
Solenoid Valve tip and/or hose &
10 x 10 x 10
storage bin up to
100 or 160 °C
Autodrop
Pipettes: No;
20 x 20 x 20
Modular heads: Micro dispenser:
Autodrop Gantry 80 x 80 x PC &
microdrop 30 x 30 x 10 Closed Inkjet – Piezo DOD, 4 365-405 nm No No nozzle Yes Automatic
II 80 included Repeatability:
Solenoid Valve tip and/or hose &
5x5x5
storage bin up to
100 or 160 °C
Resolution: Autodrop
1 x 1 x 1; Pipettes: No;
Modular heads: Positioning Micro dispenser:
Autodrop Gantry – 110 x 85 x PC
36 x 60 x 10 Closed Inkjet – Piezo DOD, 8 365-405 nm accuracy: No No nozzle Yes Automatic
AD-P-9000 190 included
Solenoid Valve 10 x 10 x 10; tip and/or hose &
Repeatability: storage bin up to
3x3x3 100 or 160 °C
Table S3. Modular Print Heads Overview for low-cost 3D bioprinters. Note: RT = Room Temperature; not all Hydrel 3D’s modular is
suitable to be listed in the table.
Microdroplet/Microline Hot- Curing
Number of Pneumatic Extruder Mechanical Extruder Camera
Model Extruder Filament Melt Melting Light
Company Available Tool
Name Extruder Electrowriting Pneumatic Tool
Modules Cooled Ambient Heated Cooled Ambient Heated Cooled Ambient Heated Head
Extruder Head
(< RT) (RT) (> RT) (< RT) (RT) (> RT) (< RT) (RT) (> RT)
Dr.
ROKIT
INVIVO 3 ✓ ✓ ✓
HEALTHCARE.
4D
Tissue ✓
3D Cultures 4 ✓
Scribe 3 options
BIO V1 / ✓ ✓
Regemat 3D 7 ✓ ✓ ✓
REG4LIFE 2 options 2 options
CELLINK BIO X 7 ✓ ✓ ✓ ✓ ✓ ✓ ✓
AXO 3D
✓
Axolotl Biosystems Bioprinter 7 ✓ ✓ ✓ ✓ ✓
2 options
Series
Brinter® – ✓
✓
Brinter® 3D 9 ✓ 2 ✓ ✓ ✓ ✓
2 options
BioPrinter options
jetlab® ✓
MicroFab 8
series 8 options
Engine ✓ ✓ ✓ ✓
Hyrel 3D 39 ✓ ✓ ✓
SR/HR 13 options 8 options 7 options 8 options
Supplemental Figures
Listed by manufacturer: (A.i) 3D Cultures, Philadelphia, PA, US; (A.ii) Advanced Solutions, Inc.,
Louisville, KY, US; (A.iii-iv) Hyrel 3D, Norcross, GA, US; (A.v) LulzBot, Fargo, ND, US; (B.i-
iii) Allevi, Inc., Philadelphia, PA, US; (B.iv-v) Regemat 3D, Granada, Spain; (C.i) ROKIT
HEALTHCARE Inc., Seoul, South Korea; ; (C.ii-v) Axolotl Biosystems Ltd., Istanbul, Turkey;
(D.i) Brinter®, Turku, Finland; (D.ii) FELIXprinters, Ijssel stein, The Netherlands; and (D.iii-v);
CELLINK, Gothenberg, Sweden. Images are adapted from the public domain websites of the
manufacturers.
Figure S2. Photographs of commercially available low-cost piezoelectric droplet-based
bioprinters. (a) Materials Printer (DMP)-2850, FUJIFILM Dimatix, Inc., Santa Clara, CA, US;
(b&c) jetlab® 4 and jetlab® 4xl inkjet workstations, MicroFab Technologies, Inc., Plano, TX, US.
Allegro 3D, San Diego, CA, US. Images are adapted from the public domain websites of the
manufacturers.