Mr.
PAWAN KUMAR 20448938 Reference: SELF VID: 240325101920331
Sample Collected At: Registered On:
VENKATESHWAR HOSPITAL (A UNIT
20/06/2024 11:10 AM
OF ASHA)
PID NO: P39224524479256 SECTOR 18A DWARKA NEW DELHI- Collected On:
Age: 49 Year(s) Sex: Male 110075 20/06/2024 11:07AM
Sample Processed At: Metropolis Reported On:
Healthcare Ltd E-21, B1 Mohan Co-op Ind
Estate New Delhi-110044 21/05/2024 08:16 AM
Investigation Observed Value Unit Biological Reference Interval
Anti HBe-Ab to Hepatitis B Envelope Ag Non Reactive(1.12) Ratio Non Reactive: > 1
(Serum,CMIA) Reactive: <= 1
Abbreviation :
CMIA : Chemiluminescence Microparticle Immunoassay
HBeAg-Hepatitis B Envelope Antigen REACTIVE(4.96) S/CO Non Reactive: < 1.0
(Serum,CMIA) Reactive: >= 1.0
Abbreviation :
CMIA : Chemiluminescence Microparticle Immunoassay
Tests marked with NABL symbol are accredited by NABL vide Certificate no MC-2676; Validity till 04-04-2026
Page 1 of 4
Dr. Chakshu Bansal
M.D (Pathology)
(DMC Reg. No. - 66994)
Mr. PAWAN KUMAR 20448938 Reference: SELF VID: 240325101920331
Client Address: Registered On:
VENKATESHWAR HOSPITAL (A UNIT
20/06/2024 11:10 AM
PID NO: P39224524479256 OF ASHA)
SECTOR 18A DWARKA NEW DELHI- Collected On:
Age: 49 Year(s) Sex: Male
110075 20/06/2024 11:07AM
Sample Processed At: Metropolis Reported On:
Healthcare Ltd E-21, B1 Mohan Co-op
Ind Estate New Delhi-110044 21/05/2024 08:16 AM
HBV-Hepatitis B Viral Load (Quantitative)
Target Selected : Highly conserved Core region of the HBVgenome across
A-H genotypes is selected for amplification & detection.
Equipment : Rotor Gene Q
Specimen Type : Serum/ EDTA Plasma
Test Principle : Real Time PCR (Taqman Probe)
Result :
HBV - Hepatitis B Viral load (Quantitative) 7,741 IU/mL
Log Value 3.11
HBV - Hepatitis B Viral load 68476 copies/ml
Result Interpretation:
Result (IU/ml) Log Value Comments
Below 31.6 IU/ml Below 1.50 Sample provided does not contain HBV DNA or HBV DNA detected
but below the lower limit of linear range of the assay. These results
should be interpreted with caution
>31.6 - 20000000 1.50 – 7.30 HBV DNA Detected within the linear range of the assay
Above 20000000 Above 7.30 HBV DNA Detected above the linear range of the assay
Note:
• This assay is a quantitative assay used for monitoring patients on therapy and not qualitative assay used
for screening. Hence a Target Not Detected result should not be considered as HBV status Negative for
the patient.
• Quantitative viral load results are best reflected when reported using log transformed units. Logarithmic
expression best reflects the process of viral replication and is less subject to over interpretation of non-
clinically significant (minor) changes.
Test Details:
Limit of Detection: 31.6 IU/ml
Measuring Range: 31.6 – 20000000 IU/ml
Conversion Factor: 1 IU/ml- 8.21 copies/ml
Dr. Shaheen.Bhat
M.D (Microbiology)
Page 2 of 4 (DMC Reg. No. - 20785)
Mr. PAWAN KUMAR 20448938 Reference: SELF VID: 240325101920331
Client Address: Registered On:
VENKATESHWAR HOSPITAL (A UNIT 20/06/2024 11:10 AM
PID NO: P39224524479256 OF ASHA) Collected On:
Age: 49 Year(s) Sex: Male SECTOR 18A DWARKA NEW DELHI-
20/06/2024 11:07AM
110075
Sample Processed At: Metropolis Reported On:
Healthcare Ltd E-21, B1 Mohan Co-op 21/05/2024 08:16 AM
Ind Estate New Delhi-110044
Clinical utility:
• Determine need to treat chronic HBV infection
• Indicator of chronic hepatitis
• Monitor virological response to therapy
• Demonstrate viral replication in patients with mutant HBV
• Predict likelihood of response to therapy
• Indicate emergence of resistant variants during antiviral therapy
Clinical Background:
• HBV is the most common cause of chronic liver disease worldwide. HBV is a DNA virus that is transmitted
primarily through blood exposure and sexual contact, and from mothers to their children.
• The clinical manifestations range from sub clinical hepatitis to symptomatic hepatitis and, in rare
instances, fulminant hepatitis. Long-term complications of hepatitis B include cirrhosis and hepatocellular
carcinoma.
• Perinatal or childhood infection is associated with few or no symptoms but has a high risk of becoming
chronic.
• HBV DNA detection and HBV DNA level measurement are essential for the diagnosis, decision to treat
and subsequent monitoring of patients.
• Follow-up using sensitive real-time PCR quantification assays is strongly recommended because of their
sensitivity, specificity, accuracy and broad dynamic range.
Limitation of Assay:
PCR is a highly sensitive technique; common reasons for paradoxical results are contamination during specimen
collection, selection of inappropriate specimen and inherent PCR inhibitors in the sample. Confirmed HBV cases
may have viral load below this detection range. Hence the results Below 31.6 IU/ml do not indicate that the
patient is negative for HBV. It is not advisable to compare viral loads between two different techniques.
Reference:
• EASL Clinical practice guidelines: Management of chronic hepatitis B. J Hepatol 2012; 57:167-185.
• Lok ASF, McMahan BJ, Chronic hepatitis B: Update 2009.HEPATOLOGY 2009, 50:No.3.
• WHO Hepatitis B Fact sheet N 204 July 2012.
Tests marked with NABL symbol are accredited by NABL vide Certificate no MC-2676; Validity till 04-04-2026
Page 3 of 4
Dr. Shaheen.Bhat
M.D (Microbiology)
(DMC Reg. No. - 20785)
Mr. PAWAN KUMAR 20448938 Reference: SELF VID: 240325101920331
.. Sample Collected At:
Venkateshwar Hospital (a Unit Of Asha) Registered On:
Sector 18a Dwarka New Delhi-110075 20/06/2024 11:10 AM
PID NO: P39224524479256 Processing Location:- Metropolis Collected On:
Age: 49 Year(s) Sex: Male Healthcare Ltd,Unit No409-416,4th 20/06/2024 11:07AM
Floor,Commercial Building-1,Kohinoor
Mall,Mumbai-70 Reported On:
21/05/2024 08:16 AM
Investigation Observed Value Unit Biological Reference Interval
Anti-HBc IgM (IgM Ab to Hep-B Core Reactive,31.12 S/CO Non Reactive: < 1
Ag) Reactive: >= 1
(Serum,CMIA)
Interpretation:
• Positive results indicate a recent or acute hepatitis B infection, while negative results suggest no recent infection or past
infection.
Clinical Utility
• Helps diagnose recent hepatitis B infection, differentiate acute from chronic HBV, and is the earliest specific antibody to
appear in response to HBV. Can help in the detection of surface antigen mutants.
Associated Tests:
• Hepatitis - Atypical Virus Profile (H0076), Anti HBcAg total (H0026)
Reference:
• Kit Insert
-- End of Report --
Tests marked with NABL symbol are accredited by NABL vide Certificate no MC-2139; Validity till 22-06-2024
Page 4 of 4 Dr. BHAVYA SAXENA
M.B.B.S., M.D. (Pathology)
Consultant Pathologist,
Reg No.2014/08/3484
