Student name:
Q1. After study the following figure, answer the question:
A)
1. What's the type of inhibitor? Why?
2. How can be calculated the Km form the figure?
B)
1. What's the type of inhibitor? Why?
2. How can be calculated the Km with inhibitorform the figure?
Q2: A biochemist obtains the following data for two charts for an enzyme without inhibitor and
with inhibitor that is known to follow Michaelis-Menten kinetics.
Chart A (No inhibitor) Chart B (with inhibitor)
Vo Vo
[S] [S]
According to above charts, Calculate:
1. From chart A (NO inhibitor):
(a) Vmax for the enzyme without inhibitor is ________________________________________
(b) Km for the enzyme without inhibitor is __________________________________________
2. From chart B (with inhibitor):
(a) Vmax for the enzyme with inhibitor is___________________________________________
(b) Km for the enzyme with inhibitor is_____________________________________________
3. What is the type of inhibitor?___________________________________________________
Why?________________________________________________________________________
4. What are you think? Inhibitor has the same structure or different structure of substrate.
Q3: Comparing between the competitive inhibitor and non-competitive inhibitor in:
competitive inhibitor non-competitive inhibitor
Number of site on
the enzyme
Structure of
inhibitor is similar
or different with
substrate
Km
Vmax
Q4:
1. The Function of Trypsin
______________________________________________________________________________
______________________________________________________________________________
__________
2. The function of Serratopeptidase
______________________________________________________________________________
______________________________________________________________________________
________
Q5: The rate of an enzymatic reaction is measured with three similar but different
substrates. From the experiments, the Km and kcat values were determined for each
substrate. (2 marks)
Km Kcat Kcat/Km (M-1 S-1
3
Substrate A 1 5*10 5*103
Substrate B 0.1 5*10-2 5*10-1
4
Substrate C 100 5*10 5*102
Based on this data, answer the following questions:
1. For which compound the enzyme has the highest affinity? Explain your answer.
2. With which compound the enzyme has maximum Velocity? Explain your answer.
3. With which compound the enzyme has highest catalytic efficiency? Explain your
answer
Q6. Study the following figure for the adenylyl cyclase signaling pathway, then answer the
following questions:
1) The Transducer is………………………………………………………………..…
2) The effector enzyme is………………………………………………….…………..
3) The second messenger is……………………………………………………...…….
4) The effect of Caffeine is.........................………………………………………
