Lab Report
Okay so a lab report consists of:
1. Title- what was the experiment about? (Title should be under 100 characters)
2. Abstract- a summary of the entire report, usually wrote last and has no citations or references
3. Introduction- background information (what was the experiment about?),
observations/goals/purpose (of the study) Note that information must must be cited here in
parentheses
4. Materials and methods- includes materials/equipment used and exactly what was done in the
experiment, should be in paragraph form
5. Results- should include my data in words and also in table form, I DO NOT have to interpret
data in this section
6. Discussion- should indicate whether or not the data supported my hypothesis (it’s incorrect to
say “I proved my hypothesis by….” I need to interpret the results. What does it mean in the
grand scheme of biology?
7. References- should have at least 3 primary references (scientific journal articles) cite them in
CSE format
Question
Can you write a lab report differentiating between the four classes of biological molecules and
identify positive and negative reactions that occur for each test as they are tested with their
chemicals, Benedict’s reagent, lugols reagent, Sudan IV and biuret reagent. When the
carbohydrate ( monosaccharides and some disaccharides) reacts with Benedict’s reagent it yields
a brownish orange color if the reaction is positive. When the carbohydrate (polysaccharide)
reacts with lugol’s reagent it will yield a dark purple/dark blue/ black color if it’s positive. When
the lipid reacts with Sudan IV it will yield bright red droplets if it’s positive. When the protein
reacts with Biuret’s Reagent it will yield a violet color if it’s positive. Nucleic Acids aren’t
reacted with anything band therefor have no reaction. The results of the Benedict’s reagent are as
follows: starch was a cloudy blue before boiling, after boiling it turned dark blue so no sugars
were found. Sucrose was blue before boiling and blue after boiling and no sugars were found.
Glucose was a clear/light blue before boiling and turned dark brown after boiling so sugars were
found. Then a potato was blue before boiling and turned light blue after boiling so no sugars
were found. Lugol’s Reagent are as follows: starch was cloudy before being added with Lugol’s
Reagent, after the Lugol’s Reagent was added it turned yellow meaning no starch was present.
Sucrose was clear before adding Lugol’s Reagent and turned a dark purple after Lugol’s Reagent
was added meaning starches were present. Sucrose was clear before Lugol’s Reagent was added,
after Lugol’s Reagent was added it turned yellow meaning no starch was present. Glucose was
clear before Lugol’s Reagent was added, after it was added it turned dark purple meaning starch
was present. The potato slice was yellow before Lugol’s Reagent was added, after Lugol’s
Reagent was added it turned a dark purple meaning starch was present. Sudan IV are as follows:
cooking oil and water mixed with the Sudan IV yielded light pink on top of the water. Glucose
when mixed with Sudan IV yielded a very slight pink all through the solution. The Biuret
Reagent are as follows: when egg whites (albumin) was mixed with Biuret Reagent yielded a
purple color. When glucose was mixed with Biuret Reagent it yielded a clear blue color. When
water was mixed with Biuret reagent it yielded a clear blue color. Lastly the nucleus acids were
examined via a strawberry. A squished strawberry was combined with detergent and NaCl to free
the DNA by decomposing the cell walls, then ethanol was added to bring the DNA out of the
solution- a fluffy white cloud was in between the two liquids, which was said DNA.
