Phy
Uploaded by
Nuwemuramuzi Ian MartinPhy
Uploaded by
Nuwemuramuzi Ian MartinDICUSSION OF RESULTS:
Glucose Concentration
Low glucose concentrations reduce glycolysis, impairing energy production and enhances RBC
fragility hence increased susceptibility to hemolysis.
Normal glucose concentrations maintain RBC membrane intergrity, support glycolysis, generate
sufficient energy for the RBC hence no significant hemolysis.
High concentrations increase oxidative stress, enhances glycolysis leading to increased reactive
species thus increased hemolysis. (NIDDK, 2022)
Sodium chloride concentration
Low (Hypotonic solution) leads to swelling of the cell due to water influx, increased membrane
tension, rupture and increased percentage hemolysis
Normal (isotonic) keeps the membrane intact and there is no significant hemolysis
High (hypertonic solution) leads to shrinkage due to water efflux, increased membrane rigidity,
crenation and limited hemolysis. (Bernhardt, 2003)
Urea concentration
Low and normal concentration cause no significant change in shape or volume of the cell,
maintains membrane integrity and therefore no significant hemolysis.
High concentration leads to shrinkage of the cell due to water efflux, increased membrane
rigidity, crenation and limited hemolysis. (Hoffman, J.F, 1976)