Gas Chromatography

Descriptive questions

1. Draw a block diagram for instrumentation of GC

2. Explain the principle involved in GSC and GLC.

3. Write about the derivatization methods in Gas Chromatography.

4. With a neat diagram, explain the different parts and working of Gas Chromatography.

5. Write a note on columns used in Gas chromatography.

6. Write a note on programmed temperature in Gas chromatography.

7. Explain the principle and working of detectors used in Gas chromatography.

8. Mention the carrier gases used in Gas chromatography and give their merits and

limitations.

9. Write a note on applications of Gas chromatography.

Multiple choice questions

1 Predict which of the following parameters is used in identification of an analyte in GC

Techniques?
a) Theoretical Plates
b) Retention time
c) Resolution
d) Asymmetry factor

2 Which one of the following is commonly used as a carrier gas in gas chromatography?
a) Oxygen
b) Helium
c) Carbon dioxide
d) Nitrous Oxide

3 Using constant mobile phase composition throughout the run time wall coated open tubular
columns are use in _______.
a) GC
b) TLC
c) HPLC
d) Electrophoresis

4 In Gas-liquid phase chromatography, the stationary phase is composed of _________ and the
mobile phase is made of _________.
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

5 Headspace Analysis is used to carried out analysis of volatile sample from solid and liquid in
____________?
a) High Pressure Liquid Chromatography
b) Planar Chromatography
c) Column Chromatography
d) Gas Chromatography

6 In gas chromatography, sample to be separated is converted into vapor and mixed with
gaseous M.P. and in separating GC column.
a) Component more soluble in the stationary phase travels slower
b) Component less soluble in the Stationary phase travels slower
c) Component more soluble in the stationary phase travels faster
d) Component more soluble in the mobile phase travels slower

7 For the separation of which of the following substances, Gas-solid chromatography is being
used?
a) Thermally stable organic components
 b) Volatile organic components
c) Thermally stable inorganic components.
d) Low molecular weight gaseous species

8 Which of the following is not a feature of carrier gas used in gas chromatography?
a) It must be chemically inert
b) It should be suitable for the detector employed
c) It should not be completely pure
d) It should be cheap

9 Which of the following is the disadvantage of hydrogen, which can be used as carrier gas in
gas chromatography?
a) Dangerous to use
b) Expensive
c) Reduced sensitivity.
d) High density

10 Which of the following is the disadvantage of helium, which can be used as carrier gas in gas
chromatography?
a) Dangerous to use
b) Expensive
c) Reduced sensitivity.
d) High density

11 In which of the following methods are liquid samples injected into the column in gas
chromatography?
a) Gas tight syringe
b) Micro-syringe
c) Rotary sample valve.
d) Solid injection syringes

12 What must be done to the solid samples for it to be introduced into the column without using
solid injection syringes in gas chromatography?
a) Introduced in hot-zone of the column
b) Dissolved in volatile liquids
c) Introduced using rotary sample valve
d) Introduced using sampling loops

13 Which of the following is the commonly used support material for the packed column in gas
chromatography?
a) Glass
b) Metal
c) Diatomaceous earth
d) Stainless steel

14 Capillary columns are open tubular columns constructed from which of the following
materials?
 a) Glass
b) Metal
c) Stainless steel
d) Fused silica

15 Which detector is not used in GC ?

a) Flame Ionization Detectors (FID),
b) Thermal Conductivity Detectors (TCD),
c) Electron Capture Detectors (ECD)
d) Refractive index detector.

16 In gas chromatography, what is the purpose of the stationary phase?

a) To heat the sample
b) To separate the components of the mixture
c) To act as a carrier gas
d) To detect the components

17 In gas chromatography, the separation of components is mainly based on their:

a) Size
b) Charge
c) Volatility and interaction with the stationary phase
d) Color

18 Which of the following is a sophisticated sample analysis technique for determination of

residual solvents.
a. Headspace analysis
b. Transmission Electron Microscopy
c. Spectrofluorimetric analysis
d. Dissolution Studies

19 Which of the following is the disadvantage of Hydrogen gas being used as a carrier gas in gas
chromatography.
a. Combustible nature
b. Expensive
c. Reduced Sensitivity
d. High Density

20 Name the terminology which involves increasing the temperature of a gas chromatograph
column as a function of time.
a. Isothermal run
b. Temperature programming
c. Isocratic elution
d. Gradient elution

21 Which detector is used in Gas Chromatography?

a. Electron Capture Detector
b. Bolometer
c. Photomultiplier tube
 d. Pyroelectric Detector

22 Which is not application of GC chromatography?

a) Quality control and analysis of drug products like antibiotics (penicillin), anti-viral
(amantidine), general anaesthetics, etc.
b) Polluent like formaldehyde, carbon monoxide, benzene , DDT etc.
c) Assay of drugs – purity of a compound can be determined for drugs like:-Atropine
Sulphate
d) Determination of purified water.

23 __________is the chromatographic technique which involves vaporisation of the

sample.

(a) HPLC

(b) Gas Chromatography

(c) Ion exchange chromatography

(d) Affinity chromatography

24 The separation of the components of the sample occurs by _____effects if the particles
of the adsorbent are coated with a liquid which forms a stationary phase.

(a) Partition

(b) Absorption

(c) Adsorption

(d) Tyndall

25 _________is an accurate device for reproducing the rate of the carrier gas.
(a) Soap bubble flow meter

(c) Galvanometer

(b) Katharometer

(d) Flow cytometer

26 The requirement of the liquid stationary phase in gas chromatography is ___________.

(a) Thermally stable

(c) Chemically reactive

 (b) Thermally unstable

(d) Highly viscous

27 The requirement of gas chromatographic detector is

(a) Response to undesired compounds

(b) High sensitivity

(c) Non-linear response

(d) Low sensitivity

28 __________detector is based on the alteration of the thermal conductivity of the gas.

(a) Bolometer

(c) Electron capture

(b) Flame ionisation

(d) Katharometer