BDS - Practical Exam Questions
General Microbiology
1. Identify the culture media, its classification, use in microbiology ( NA, MAC, BA, CA,
MHA, SDA, TSI, CITRATE, UREA, PPA, INDOLE-PW )
2. Identify the transport media – Sterile & Unsterile container, VTM, Swabs, plain
vacutainer
3. Gram Stain – Principle, Procedure, Advantages, Disadvantages, Examples of GN/GP
organisms, Importance, Modifications, theories of Gram stain
4. ZN stain - Principle, Procedure, Advantages, Disadvantages, Examples of acid fast
organisms, Importance, Modifications, RNTCP grading
5. Different types of Microscope-their principle, stains, reagents, examples of bacteria
6. Examples of GNB, GPB, GPC,GNC
7. Autoclave- Principle, uses, advantages, disadvantages,
8. Biological and chemical indicator
9. Content of sterilium, Dettol, savlon- their uses, MOA
10. Different methods of sterilization & Disinfection
Bacterial Identification:
1. Sample collection & transport
2. Biochemical test for Gram negative bacilli
3. Biochemical reaction for Gram positive cocci
4. Indole test – Positive and Negative with one or two example.
5. Citrate test – Positive and Negative with one or two example.
6. Urease test – Positive and Negative with one or two example.
7. Triple Sugar Iron (TSI) test – Positive and Negative with one or two example.
8. Phenylalanine agar (PPA) test – Positive and Negative with one or two example.
9. Automated identification methods.
Antimicrobial Susceptibility testing
1. Media use commonly for AST.
2. Different AST methods
3. MIC & MBC definition.
4. Different automated AST methods.
Immunology
1. Identify – ELISA plate ( ELISA full form, types, principles, examples of tests done by
ELISA method), Rapid test cards ( HIV, HBsAg, HCV, Dengue, WIDAL, RPR, LA – Its
principles, use and examples of each)
2. Serological Reactions ( Ag-Ab reactions- types, principles, examples)
3. Widal test- Interpretation, reason for false positive , anamnestic reaction,
Serological reactions
1. Definition of precipitation and Examples.
2. Definition of Agglutination and Examples.
3. Widal test-principle and method and identification of Tile
4. RPR-Principle and identification of card
5. Latex agglutination test- Example ,method and Identification of card
6. Rapid Immunochromatography test by Lateral flow assay – Identification and principle.
7. Rapid Immunochromatography test by Flow Through assay – Identification and
principle.
8. ELISA (Microtiter plate)- Identification ,types and use.
Bacteriology
1. colour of staphylococcus colony, interpretation of gram stain
2. morphology of staphylococcus, streptococcus, C. diphtheriae
3. infection caused by staphylococcus and streptococcus
4. morphology of N. Meningitidis and N. Gonorrhoae
5. Diseases caused by C. diphtheriae
6. specific stain and media used for C. diphtheriae
7. gas gangrene caused by which microorganism
8. which vaccine is used for prevention of tetanus
9. types of UTI
10. what is the difference between upper UTI and lower UTI
11. what are sign and symptoms of enteric fever
12. Gold standard method for detection of salmonella typhi
13. what are the other tests for detection of salmonella typhi – first ,second, third & forth
week
14. Widal test- Interpretation, reason for false positive , anamnestic reaction.
15. Various laboratory diagnostic method for Staphylococcus, Streptococcus ,
Mycobacterium tuberculosis etc.
Virology
1. Rapid Immunochromatography test by Lateral flow assay – Identification and
principle for HIV, Hepatitis viruses etc.
2. Rapid Immunochromatography test by Flow Through assay – Identification and
principle for HIV, Hepatitis viruses etc.
3. ELISA (Microtiter plate)- Identification ,types and use.
4. Mode of transmission and various laboratory diagnostic method for HIV, Hepatitis
viruses, Herpes viruses
Parasitology
1. Classification of various parasites, examples of nematodes, amoeba etc.
2. Non bile stained egg example.
3. Stool concentration techniques.
Mycology
1. Classification of fungi, selective media for fungus?
2. What is dimorphic fungi?
3. What is yeast?
4. What is mold?
5. Which reagent use for fungal microscopy?
6. Which culture media used for fungal culture?
7. Candida identification test
8. Give 3 names of Opportunistic fungus.
