.-.-..
CLUSTER U
� � NIVERSITY SRINAGAR
us (FYUP UNDER NEP 2020)
:,i �
+. Of��� Y Department of Biotechnology
oa1:Eli\.\\l,
'f-� f: . �-#
',!. Semester 3 rd (MaJo
· r/M'mor course)
Course Title·•
Molecular Cell Biology
C ourse Cod . U .
GB
Max. Marks\so TC23JJ01/ UGBTC23N301
e red'•ts: 6 (Theory: 4, Practicrtl· 2)
C
ontact H rs: 120 (Theory: 60, Practic Theory External: 80; Min Marks: 32
al: 60)Theory lntcrnaJ (Continuo u s Assessment): 20 Marks, Min Marks: 08
PracticaJ Ex1>erimental Basis= 30, Min. Marks: 12
PracticaJ Expc1imc11tal (Continuous assessment) 20, Min. Marks: 08
· ·
Objectives·· This course 1s ·
atmed to intr o duce students to basic concepts of Cell Biology.
Learning Outcomes: Students will be able t o :
► Students will be able to have an insight of cell as the fundamental unit of life.
► An�yze the structure and :function of different cell organelle.
► �am an understand ing of the functions performed by the cytoskeleton and the significance of cell-cell.
mteractions and distinguish between different phases of cell cycle.
UNIT I 15 hours
Introducti o n to Cell (Animal and Plant Cell), Cell Membrane-Structure and Function. Membrane Organizati on (Fluid
+ +
M o saic Model). Transport across membrane-Active and Passive Transport (Ca++ -ATPase, Na /K · ATPase,
Na++Linked Antiporter, ca++ from Cardiac Muscle, Symp o rters, Diffusion and Facilitated Diffusion).
UNIT II 15 hours
Endoplasmic Reticulwn, Golgi Complex and Lysosomes: structure and functi on, Role in Protein so rting and
Transport Mechanism of Vesicular transport (COP I, COP II and Clathrin coated Vesicles). Endocytosis, Pinocytosis
and Phagocytosis
UNIT III 15 hours
Mitochondria, Chloroplast, Ribosomes, Vacuoles and Peroxiso mes: Structure and Function. Structure and
Organization of Nucleus, Organization of Nuclear Pore. Structure and Functio ns of Microtubules, Microfilaments
and Intermediate Filaments
UNIT IV 15 hours
Extra Cellular matrix and cell matrix interactions. Cell- Cell interactions : Adherence junctions, tight junctions, gap
junctions, desmosomes, hemidesmosomes, fooaJ adhesions and plasmodesmata. Cell cycle (mitosis & meiosis),
regulation of cell cycle. Apoptosis.- brief idea
Practical/Lab Course, (Credits=2) 60Hrs
1. Studying of different cellular organelles with animations and micrographs
2. S tudying the different stages of mitosis by preparing slides of onion root tip.
3. Staining of cells.
onic and hypertomc
4. Observations on the penneability of the pJasma membrane- effect of isotonic, hypot
solutions on mammalian RBCs or any other cel1.
5. Field trip/subject tour/report
SUGGESTED READING: . New York USA
1. Molecular Biol ogy of the Cell by Albe rts, B Taylor and Fran cis,
Karp, John Wiley & Sons: . Philadelphia.
2. Cell and Molecular Biology: Concepts and. Experiments by. G. . .
3. Cell and Molecular Biology by De Robert:Is and De
Robertis L1ppmcott 1ll · w·
iams and Wtlk
JJ1S,
I
. and Hausman ASM Pre s.
4. The Cell: A Molecular Approach by Cooper, G.M
Publisher,
5. C.B.Powar (2010). Cell Biology, Himalaya
