Course Outline

School: Eng. Tech. & Applied Science

Department: Applied Biological and Environmental

Sciences (ABES)

Course Title: Biochemistry 1

Course Code: CH 222

Course Hours/Credits: 70

Prerequisites: CH 121

Co-requisites: N/A

Eligible for Prior Learning, Yes

Assessment and Recognition:

Originated by: Connie Zehr

Revised by: Fouroozan Mohammadi

Revision Date: Summer 2014

Current Semester: Winter 2024

Approved by:

Chairperson/Dean

Students are expected to review and understand all areas of the course outline.

Retain this course outline for future transfer credit applications. A fee may be charged for
additional copies.

This course outline is available in alternative formats upon request.

CH 222 CENTENNIAL COLLEGE Biochemistry 1

Acknowledgement of Traditional Lands

Centennial is proud to be a part of a rich history of education in this province and in this city. We
acknowledge that we are on the treaty lands and territory of the Mississaugas of the Credit First Nation
and pay tribute to their legacy and the legacy of all First Peoples of Canada, as we strengthen ties with
the communities we serve and build the future through learning and through our graduates. Today the
traditional meeting place of Toronto is still home to many Indigenous People from across Turtle Island and
we are grateful to have the opportunity to work in the communities that have grown in the treaty lands of
the Mississaugas. We acknowledge that we are all treaty people and accept our responsibility to honor all
our relations.

Course Description
This course introduces you to theoretical and practical applications in Biochemistry, and prepares
students for employment in food, pharmaceutical, and beverage manufacturing as well as government
and private laboratories. The theory focuses on important molecules found in living systems. These
concepts are then applied in the laboratory. Skills developed in prior courses (Analytic and Organic
Chemistry) will be further developed upon in this course.

Program Outcomes
Successful completion of this and other courses in the program culminates in the achievement of the
Vocational Learning Outcomes (program outcomes) set by the Ministry of Colleges and Universities in the
Program Standard. The VLOs express the learning a student must reliably demonstrate before
graduation. To ensure a meaningful learning experience and to better understand how this course and
program prepare graduates for success, students are encouraged to review the Program Standard by
visiting http://www.tcu.gov.on.ca/pepg/audiences/colleges/progstan/. For apprenticeship-based programs,
visit http://www.collegeoftrades.ca/training-standards.

Course Learning Outcomes

The student will reliably demonstrate the ability to:
1. Describe the structures and functions of all cellular components. A discussion of water and buffer
and their properties will also be included
2. Explain the structure, function and chemistry of all biomolecules. DNA replication and protein
synthesis will also be included
3. An introduction to Enzyme function and Kinetics
4. Draw structural formulas including Fischer and Haworth projection formulas for these basic
biomolecules and their derivatives
5. Relate the physical characteristics and behavior of biomolecules to their molecular structure
6. Predict chemical reactions on the basis of their structural and molecular formulas
7. Document experimental data in a variety of reports as outlined in the laboratory manual
8. Apply a variety of lab skills during lab activities in a safe and responsible manner
9. Interpret and validate experimental data to develop appropriate conclusions

Essential Employability Skills (EES)

The student will reliably demonstrate the ability to*:

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

1. Communicate clearly, concisely and correctly in the written, spoken, and visual form that fulfills the
purpose and meets the needs of the audience.
2. Respond to written, spoken, or visual messages in a manner that ensures effective
communication.
5. Use a variety of thinking skills to anticipate and solve problems.
9. Interact with others in groups or teams in ways that contribute to effective working relationships
and the achievement of goals.
*There are 11 Essential Employability Skills outcomes as per the Ministry Program Standard. Of these 11 outcomes, the following will be
assessed in this course.

Global Citizenship and Equity (GC&E) Outcomes

N/A

Methods of Instruction
Two hours per week delivered via in-class lecture. One hour per week delivered via online learning. Two
hours per week are in the laboratory.

Text and other Instructional/Learning Materials

Text Book(s):
SaplingPlus for Lehninger Principles of Biochemistry, 7th Edition.

Laboratory Manual for CH222: Biochemistry I, prepared by Centennial College Professors.

Online Resource(s):
CH 222 In-class lecture notes, online lecture activities, and CH 222 Lab Manual- most will be available
through eCentennial
Material(s) required for completing this course:
SaplingPlus for Lehninger Principles of Biochemistry; CH 222 lecture notes and Lab manual posted on
eCentennial; access to a computer and internet for completing online component. Safety Glasses, Full-
length lab coat, closed toe/heel covered flat shoe, a lab kit and locker rental are mandatory for laboratory
sessions.

Evaluation Scheme
➮ Test # 1: To be announced through eCentennial.
➮ Test # 2: To be announced through eCentennial.
➮ Test # 3: To be announced through eCentennial.
➮ Laboratories: Based on Written Lab reports and Lab Exam
➮ Achieve Online Activities: Online Activities.
Evaluation Name CLO(s) EES GCE Weight/100
Outcome(s) Outcome(s)
Test # 1 1, 2, 4 1, 2, 5 10
Test # 2 2, 4, 5, 6 1, 2, 5 10
Test # 3 2, 3, 4, 6 1, 2, 5 10
Laboratories 6, 7, 8, 9 1, 2, 5, 9 40
Achieve Online Activities 2, 5, 8, 9 5, 9 30
Total 100%
If students are unable to write a test they should immediately contact their professor or program Chair for

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

advice. In exceptional and well documented circumstances (e.g. unforeseen family problems, serious
illness, or death of a close family member), students may be able to write a make-up test.

All submitted work may be reviewed for authenticity and originality utilizing Turnitin®. Students who do not
wish to have their work submitted to Turnitin® must, by the end of the second week of class,
communicate this in writing to the instructor and make mutually agreeable alternate arrangements.

When writing tests, students must be able to produce official Centennial College photo identification or
they may be refused the right to take the test or test results will be void.

Tests or assignments conducted remotely may require the use of online proctoring technology where the
student’s identification is verified and their activity is monitored and/or recorded, both audibly and visually
through remote access to the student's computer and web camera. Students must communicate in writing
to the instructor as soon as possible and prior to the test or assignment due date if they require an
alternate assessment format to explore mutually agreeable alternatives.

Student Accommodation
The Centre for Accessible Learning and Counselling Services (CALCS) (http://centennialcollege.ca/calcs)
provides programs and services which empower students in meeting their wellness goals,
accommodation and disability-related needs. Our team of professional psychotherapists, social workers,
educators, and staff offer brief, solution-focused psychotherapy, accommodation planning, health and
wellness education, group counselling, psycho-educational workshops, adaptive technology, and peer
support. Walk in for your first intake session at one of our service locations (Ashtonbee Room L1-04,
Morningside Room 190, Progress Room C1-03, The Story Arts Centre Room 285, Downsview Room 105)
or contact us at [email protected], 416-289-5000 ext. 3850 to learn more about accessing
CALCS services.

Use of Dictionaries
• Dictionary use is not permitted in test or examination settings.

Program or School Policies

N/A

Course Policies
• Attendance in all laboratory sessions is mandatory.
• Students are required to attend at least 80% of lectures
• Students require a locker. Contact Enrolment Services for rental at Morningside campus.
• Students require a full-length laboratory coat and flat-soled shoes with closed toes and heels.
• Safety glasses will occasionally be required.
• Students must adhere to the SETAS “Lab Responsibilities and Safety Policies”.
• Students are required to be familiar with and adhere to all Biohazard Level 2 Laboratory Safety Rules
when working in the laboratory. Students who are absent during experimental procedures will not be
permitted to hand in the corresponding reports. The marks for these reports will not be available to these

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

students.
• Students will not be permitted to enter the laboratory without a copy of the appropriate lab protocol(s)
each week.
• Students must obtain at least 50% in the theory component and at least 50% in the laboratory
component to successfully pass this course.
• A grade of C (60%) in this subject is required for entry into the 3rd year of the Technologist program.
• A grade of C (60%) in this subject is required for graduation from the Technologist (Advanced) Diploma.
• A grade of C (60%) in this subject is required for entry into and graduation from the 3rd year of the
Technologist program.

Students must complete laboratory maintenance and monitoring duty to the satisfaction of the professor
and lab technologist. Students are required to attend all labs and 80% of lectures: attendance will be
taken. The labs are usually performed in groups of two students. Usually, the tasks required for each lab
will be divided up within the group. Results are to be shared within the group and are reported by each
student. In the report, it should be stated which results are produced by the author and which ones were
generated by co-workers.

• Students must complete laboratory maintenance and monitoring duty to the satisfaction of the professor
and lab technologist. A failing (F) grade will be assigned if lab duties are not completed satisfactorily.
Marks may be deducted for lab reports submitted after the due date at a rate of 10% per day (weekends
included.
• Laboratory reports are due one week after the exercise has been completed.
• Laboratory schedule is tentative and may vary due to unforeseen circumstances. As a result, topics and
experiments may be added, deleted or rearranged.

College Policies
Students should familiarize themselves with all College Policies that cover academic matters and student
conduct.

All students and employees have the right to study and work in an environment that is free from
discrimination and harassment and promotes respect and equity. Centennial policies ensure all incidents
of harassment, discrimination, bullying and violence will be addressed and responded to accordingly.

Academic Honesty

Academic honesty is integral to the learning process and a necessary ingredient of academic integrity.
Forms of academic dishonesty include cheating, plagiarism, and impersonation, among others. Breaches
of academic honesty may result in a failing grade on the assignment or course, suspension, or expulsion
from the college. Students are bound to the College’s AC100-11 Academic Honesty and Plagiarism
policy.

To learn more, please visit the Libraries information page about Academic Integrity
https://libraryguides.centennialcollege.ca/academicintegrity and review Centennial College's Academic
Honesty Module:
https://myappform.centennialcollege.ca/ecentennial/articulate/Centennial_College_Academic_Integrity_M

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

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Use of Lecture/Course Materials

Materials used in Centennial College courses are subject to Intellectual Property and Copyright
protection, and as such cannot be used and posted for public dissemination without prior permission from
the original creator or copyright holder (e.g., student/professor/the College/or third-party source). This
includes class/lecture recordings, course materials, and third-party copyright-protected materials (such as
images, book chapters and articles). Copyright protections are automatic once an original work is created,
and applies whether or not a copyright statement appears on the material. Students and employees are
bound by College policies, including AC100-22 Intellectual Property, and SL100-02 Student Code of
Conduct, and any student or employee found to be using or posting course materials or recordings for
public dissemination without permission and/or inappropriately is in breach of these policies and may be
sanctioned.

For more information on these and other policies, please visit www.centennialcollege.ca/about-
centennial/college-overview/college-policies.

Students enrolled in a joint or collaborative program are subject to the partner institution's academic
policies.

PLAR Process
This course is eligible for Prior Learning Assessment and Recognition (PLAR). PLAR is a process by
which course credit may be granted for past learning acquired through work or other life experiences. The
PLAR process involves completing an assessment (portfolio, test, assignment, etc.) that reliably
demonstrates achievement of the course learning outcomes. Contact the academic school to obtain
information on the PLAR process and the required assessment.

This course outline and its associated weekly topical(s) may not be reproduced, in whole or in
part, without the prior permission of Centennial College.

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

Semester: Winter 2024 Professor Name: Fouroozan Mohammadi

Section Code: CH222 Contact Information: 58281
Meeting Time & Location: Online Lectures on Monday and Thursday Office Hours: It will be announced
Delivery Method: online lecture and labs on campus Additional Information: Materials required for completing this course:

Achieve Online activities(Lehninger Principles

of Biochemistry,8th Edition) and
Principles of Biochemistry. David L. Nelson
and Michael M. Cox Lehninger, Freeman 8th
edition.
The lecture portion is online and the lab
portion is on Campus.

Topical Outline (subject to change):

Week Topics Readings/Materials Weekly Learning Outcome(s) Instructional Evaluation Evaluation
Strategies Name and Date
Weight
1 Lecture: Textbook Lecture: Define Biochemistry and identify Lecture online Lecture: Term TBD
Water and Buffers Chapter 2 course learning outcomes Test 1
Achieve online Lecture Notes Describe the polarity and solvent properties of Lab: Introduction and
activity water molecule. Discussion Lab: Lab Quiz
Lab manual from (Lab safety
Lab: eCentennial, Lab: Demonstrate biosafety procedures and mandatory)
Lab introduction Biosafety instructions, discuss lab experiments and lab report
Lab Safety Lab policies and Lab requirements
Report Guidelines
2 Lecture: Textbook Lecture: Lecture online Lecture: Term TBD
Water and Buffers Chapter 2 Describe the importance of the Hydrogen Test 1
Achieve online Lecture Notes from Bond. Lab: Teacher-led
activity eCentennial Review Acids and Bases instructions, lab Lab: Lab
Achieve online activity Discuss Buffers experiment Report
Lab:
Lab # 1 Lab: Lab: Perform a classic Titration of Glycine
Titration of Amino Lab manual from amino acid with strong Base to explain the
Acid eCentennial Titration curves and determine the
(week 1) PKa and PI of Glycine
3 Lecture: Textbook Describe the Titration Curves Lecture online Lecture: Term TBD
Amino Acids Chapter 3 Explain Buffers, the importance of buffers, Test 1
Achieve online Lecture Notes from making the buffer and buffer selection Lab: Teacher-led
activity eCentennial Describe the structures and properties of the instructions, lab Lab:Lab report
Lab: Achieve online activity individual Amino Acids including the peptide experiment

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

Week Topics Readings/Materials Weekly Learning Outcome(s) Instructional Evaluation Evaluation

Strategies Name and Date
Weight
Lab: bond.
Lab # 1 Lab: Explain the Three-dimensional structure
Titration of Amino Lab manual from and the physiological properties of amino acid
Acid eCentennial
(week 2) Lab: Perform a classic Titration of unknown
amino acid with strong Base to explain the
Titration curves and determine the
PKa and PI of unknown aminoacid
4 Lecture: Textbook Explain the Three-dimensional structure of Lecture online Lecture: Term TBD
Proteins Chapter 4 Proteins and levels of structure in Proteins. Test 1
Achieve online Lecture Notes from Determine the relationship between protein Lab: Teacher-led
activity eCentennial structure and protein function instruction, lab Lab: Lab
Lab: Achieve online activity experiment report
Lab # 2 Lab:
Separation and Lab: Lab manual from Perform a classic separation technique using
Identification of eCentennial paper chromatography of both known and
Amino Acids unknown Amino acids to explain the
stationary and mobile phases and determine
the Rf values as well.
Identify certain protein constitution by
performing some chemical Tests
5 Lecture: Content of Lectures 1- Test knowledge and comprehension of in Lecture: Test Lecture: Term TBD
Term Test 1 4 in class and class and online activities 1-4 Test 1
Achieve online Content of Achieve Lab: Teacher-led
activity online activities Lab: Perform the separation of Lactose instructions, Lab Lab: Lab
(sugar) from other proteins using skim milk by experiment report
Lab: Lab: Lab manual from examining different separation techniques,
Lab # 3 eCentennial determine the percentage yield, collect data,
Isolation of Lactose report and interpret data
and Casein
(Week 1)
6 Lecture: Text book Describe the chemical composition of nucleic Lecture online Lecture: Term TBD
Nucleic acids Chapter 25 acid. Explain the denaturation of DNA and Test 2
Achieve online Lecture Notes from describe the five different principals of RNA Lab:
activity eCentennial Teacher-led Lab: Lab
Achieve online activity Describe the biosynthesis of Nucleic Acids instructions, Lab report
Lab: (Replication) experiment
Lab # 3 Lab: Lab manual from
Isolation of Lactose eCentennial Lab: Perform the separation of Lactose
and Casein (sugar) from other proteins using skim milk by
(Week 2) examining different separation techniques,
determine the percentage yield, collect data,

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

Week Topics Readings/Materials Weekly Learning Outcome(s) Instructional Evaluation Evaluation

Strategies Name and Date
Weight
report and interpret data
7 Lecture: Text book Describe the transcription of Genetic code Lecture Online Lecture: Term TBD
Nucleic acids Chapter 26 (the biosynthesis of RNA) in both Eukaryotes Test # 2
Achieve online Lecture Notes from and Prokaryotes. Lab: Teacher-led
activity eCentennial instructions, Lab Lab: Lab
Lab # 4 Achieve online activity Lab: experiment report
Characterization of Perform the extraction of DNA from dried
DNA Lab: Lab manual from peas by using General basic methods
(Week 1) eCentennial
8 Lecture: Text book Describe the Translation of the Genetic Lecture Online Lecture: Term TBD
Nucleic acids Chapter 27 Message (Protein synthesis) in both Test # 2
Achieve online Lecture Notes from Eukaryotes and Prokaryotes Lab: Teacher-led
activity eCentennial instructions, Lab Lab: Lab
Lecture: Achieve online activity Lab: Perform several DNA identification Tests experiment Report, Lab
Lipids and Biological on commercial DNA and RNA product and exam
Membranes Lab: Lab manual from Examine the Isolated Lab DNA by performing
eCentennial the same identification Test
Lab # 4
Characterization of
DNA
(Week 2)
9 Lecture: Text Book Describe the structures, classification and Lecture Online Lecture: Term TBD
Lipids and Biological Chapter 10 properties of different lipids types. Describe Test # 2
Membranes Lecture Notes from the nature of biological membrane. Explain Lab: Teacher-led
Achieve online eCentennial the lipid soluble vitamins, prostaglandins, instructions, Lab Lab: Lab
activity Achieve online activity Leukotrienes and their functions. experiment Report
Lab: Perform different chemical reactions of
Lab # 5 Lab: Lab Manual from Lipids to distinguish the different types of
Properties of Lipids eCentennial Lipids and to detect the composition of
different lipids.
Accurately collect report and interpret data
10 Lecture: Content of Lectures 5- Lecture: Test knowledge and Comprehension Lecture : Test Lecture: Term TBD
Term Test # 2 8 of Lecture 5-8, Achieve online activity Test # 3
Content of Achieve Lab: Teacher-led
Lab # 6 online activity Lab: Perform different chemical tests to instruction, Lab Lab: Lab
Properties of characterize monosaccharide, disaccharides experiment Report
carbohydrates Lab: Lab Manual from and polysaccharides.
(week 1) eCentennial
11 Lecture Chapter 7 Describe the structure and the Lecture in class Lecture Term TBD
Carbohydrates Lecture Notes from stereochemistry of monosaccharides and Achieve Online Test # 3
Achieve online eCentennial cyclic structure and anomers. activities and

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CH 222 CENTENNIAL COLLEGE Biochemistry 1

Week Topics Readings/Materials Weekly Learning Outcome(s) Instructional Evaluation Evaluation

Strategies Name and Date
Weight
activity Achieve online Define the reducing sugar and oxidation and Carbohydrates Lab: Lab
(Week 1) activities reduction reactions report
Carbohydrates
No lab No lab
Good Friday Lab: Lab manual from
eCentennial
12 Lecture Text Book Describe the structure and functions of Lecture: In class Lecture: Term TBD
Carbohydrates Chapter 7 different carbohydrates. lecture Test# 3
(Week 2) Lecture Notes from Describe some important oligosaccharides Achieve online activity
Carbohydrates eCentennial Describe the structure functions of Carbohydrates Lab: lab report
Achieve online Achieve online polysaccharides Simulation
activity activities
Carbohydrates Lab: Determine the composition of an Lab: Teacher-led
Lab # 6 unknown by using the information obtained in instruction, Lab
Properties of Lab: Lab manual from week 1 experiment
carbohydrates eCentennial Accurately collect, report and interpret the
(week 2) results
13 Lecture: Text Book Describe the catalytic properties of enzymes. Lecture: In class Lecture: Term TBD
Enzymes Chapter 6 Describe the Kinetics and the thermodynamic lecture Test # 3
Lecture Notes from aspects of reactions. Achieve online activity
Lab # 7 eCentennial Describe the binding between Enzyme and
Enzymes Achieve online activity Substrate. Lab: Teacher-led
Explain the Michaelis-Menten model. instruction
Lab: Lab manual from Describe Enzyme inhibition
eCentennial
Lab: Examine the actions of four enzymes,
Pepsin, Amylase and Catalase and invertase.
14 Lecture: Term Test # Content of Lectures Lecture: Test Knowledge and Comprehension Test Test # 3 TBD
3 10-12 of Lectures 10-12 Lab Exam
Lab: Content of Achieve
Lab Exam Online activities
Lab: Test Knowledge
of Labs 1-7

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