Name: ______________________________ Class: __________
Practical Assignment 5.2
Table 1.1 shows boxes for the full set of adult human teeth (32 in total).
Table 1.1
Use the mirror provided to observe your own teeth.
(a) (i) Count the number of teeth you have and record it below.
Number of teeth __________ [1]
(ii) Make a record of your own teeth by putting a ‘X’ for each tooth present in the appropriate box in
Table 1.1. [1]
(iii) Explain briefly why the third (last) molars may not be present in teenagers.
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_____________________________________________________________________________ [1]
(iv) Outline the functions of teeth during the intake of food.
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_____________________________________________________________________________ [2]
Dental hygiene is important for healthy gums and teeth.
• Rub the two sterile (unused) cotton buds around the inside of your mouth over your gums and teeth.
• Dip one cotton bud into the sugar provided so that it sticks to the surface of the cotton bud.
• Leave the other cotton bud without sugar.
• Lay both in the shallow dish provided, the one without sugar on the left.
• Add 2 or 3 drops of universal indicator solution to each cotton bud.
(b) (i) In Table 1.2, record the colour of the indicator solution immediately after it was put on to the cotton
buds.
Table 1.2
colour at start colour after 30 minutes
cotton bud without sugar
cotton bud with sugar
• Leave the cotton buds in the dish and observe after 30 minutes.
CONTINUE WITH THE REST OF THE PAPER DURING THIS 30 MINUTES.
Then, in Table 1.2, record the colours of the indicator solution after it has been on the cotton buds for 30
minutes. [2]
(ii) Use the colour chart provided to help you explain the observations you have recorded in Table 1.2.
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_____________________________________________________________________________ [3]
Saliva in the mouth may contain an enzyme, amylase, that acts on the substrate, starch.
(c) Describe how you could carry out a test to show the presence of
(i) the substrate,
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_______________________________________________________________________________
_____________________________________________________________________________ [3]
(ii) the product of the action of amylase on starch.
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_____________________________________________________________________________ [4]
You are provided with amylase and starch solutions in labelled test-tubes.
Read through the following instructions and then carry them out.
• Pour a small amount of amylase solution into two clean test-tubes.
• Carry out the test for the presence of starch in one tube and the test for the product in the other tube.
• Pour a small amount of starch solution into two clean test-tubes.
• Carry out the test for the presence of starch in one tube and the test for the product in the other tube.
Note: Make sure that you leave sufficient untested amylase and starch solution to complete the rest of this
experiment.
(d) (i) Record the results of this first set of tests in Table 1.3 below.
Table 1.3
test test for starch test for product
amylase
first set
starch
second set
with salt added
second set
without salt added
Read through the following instructions and carry them out.
• Label a clean test-tube S.
• In a large test-tube mix together the remainder of the amylase and starch solutions you were given.
• Shake the mixture and pour half of it into test-tube S.
• To this tube (S), add 4 drops of sodium chloride (salt) solution.
• Shake both tubes of mixture.
• Using clean test-tubes, test the mixture in tube S (with added salt) and the mixture in the other tube
(without added salt) for both starch and the product.
• Record your results in Table 1.3. [5]
(ii) Suggest explanations for your results recorded in Table 1.3.
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_____________________________________________________________________________ [3]
[Total: 22]
