Wesleyan University – Philippines

Mabini Extension, 3100 Cabanatuan City, Philippines

ALOE VERA AND LEMON ZEST AS AN ANTIBACTERIAL HAND SANITIZER

In partial fulfillment of the requirement for the subject

Practical Research 2

Dela Cruz, Mark Stephen F.

Dimalanta, Marry Jhoy A.

Dolormente, Ma. Jurlet P.

Estella, Christian Will L.

Javate, Eunice B.

Padilla, Rustyn C.

Reyes, Alexandria Jaye C.

Reyes, Nicole P.

Salonga, Karyll O.

Researchers

Pereña, John Rusty T.

Research Adviser

December, 2019
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CHAPTER I

INTRODUCTION

Lemon (Citrus lemon) is a common citrus fruit, alongside

grapefruits, limes, and oranges. While the pulp and juice are used the

most, the peel tends to be discarded. However, studies have determined

that lemon peel is also known as lemon zest. Zest is the outermost

rind of citrus fruits (usually lemons and oranges). It is valued for

the strong citrus flavor it imparts to food, thanks to an abundance of

perfume, aromatic oils in the rind. It is full of bioactive compounds

that may provide numerous health benefits.

Lemon zest has high nutritional value due to having a high amount

of fiber and vitamin C, providing 9% of the DV in only 1 tablespoon.

Lemon zest offers several antioxidants, including D-limonene and

vitamin C, that protects your immune system and reduce your risk of

disease. Lemon peel may offer antimicrobial and antifungal effects —

even against antibiotic-resistant strains.

Flavonoids, vitamin C, and pectin in lemon peel may promote heart

health by lowering blood cholesterol levels and other risk factors for

heart disease. Lemon peel has various applications as a household

cleaner or beauty product. Lemon peel has no reported side effects and

is recognized by the FDA as safe for human consumption. Although lemon

peel normally gets thrown away, research shows that it possesses

numerous health benefits. Its fiber, vitamin, and antioxidant contents

 2 | PAGE

may support oral, immune, and heart health. It may even have several

anticancer properties (Ariane Lang, 2019).

On the other hand, Aloe Vera is a popular medicinal plant that has

been used for thousands of years. It is best known for treating skin

injuries, but also has several other beneficial effects on health.

Aloe Vera is a thick, short-stemmed plant that stores water in its

leaves. Each leaf is full of a slimy tissue that stores water, which

makes the leaves thick. This slimy, water-filled tissue is the "gel" we

associate with Aloe Vera products. The gel contains most of the

bioactive compounds in the plant, including vitamins, minerals, amino

acids and antioxidants.

Aloe Vera gel contains powerful antioxidants, which belong to a

large family of substances known as polyphenols. These polyphenols,

along with several other compounds in Aloe Vera, can help inhibit the

growth of certain bacteria that can cause infections in humans. There

is some preliminary evidence that topical Aloe Vera gel can slow aging

of the skin. There is an evidence that Aloe Vera can treat skin

conditions like psoriasis and radiation dermatitis. Early evidence

suggests that Aloe Vera may have anti-aging effects on the skin.

Various Aloe Vera products are available to purchase in health

food stores or online, including Aloe Vera gel, juice, supplements,

oil, and powder. Aloe Vera definitely has some unique therapeutic
 3 | PAGE

properties, especially when applied as an ointment for the skin (Joe

Leech, 2017).

Conceptual Framework

Figure 1 below shows that the lemon zest and aloe vera gel can

be an anti-bacterial sanitizer.

 Aloe Vera • Extraction of

the gel from
 An
 Lemon Zest the Aloe Vera
plant. effectiveness
Lemon zest and of anti-
 Staphylococcus • Gathering the bacterial
Aureus
aloe vera gel zest from the hand
(Bacteria) lemon. sanitizer.
• Combining the
Aloe Vera gel,
lemon zest, and
other
ingredients.

INPUT PROCESS OUTPUT

Figure 1. The Conceptual paradigm of Lemon Zest and Aloe Vera gel

as an anti-bacterial sanitizer.
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Objectives of the Study

This study aimed to determine the efficacy of Aloe Vera and Lemon Zest

as an anti-bacterial hand sanitizer. Specifically, it aimed to:

1. Test the efficacy of Aloe Vera and Lemon Zest as an anti-bacterial

hand sanitizer and described in terms of inhibiting the growth of

Staphylococcus Aureus.

Significance of the Study

This study is important to the environment. It helps people free from

germs and make their hands clean. This study can also help the students

by expanding ones knowledge.

To the community, this study will benefit the people in a way that they

can use an antibacterial hand sanitizer that is more convenient, much

effective and cheap to clean their hands instead of using chemical based

antibacterial sanitizer.

To the researchers, the outcome of the study may serve as an eye-opener

to the researcher's mind to improve their skills and knowledge in

conducting a research paper.

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To the future researchers, this serves as a guide for them to conduct

a related study and be enlightened with the data gathered which will be

beneficial to their future use.

Scope and Delimitations

This study reviews different and studies related to Aloe Vera gel

and lemon zest. The efficiency of Aloe Vera gel as antibacterial and

Lemon zest as a good moisturizer. The researchers use Kirby Bauer Method

to determine the smallest inhibitory concentration of an antibacterial

drug that will inhibit during the incubation. The reason why this method

is used is to know if the bacteria are susceptible or resistant. This

study shows the properties of Aloe Vera gel and Lemon zest to kill

bacteria. The research aims to prove the efficiency of the Aloe Vera

gel and Lemon zest as an antibacterial sanitizer. The boundary of the

studies is if the Aloe Vera gel and Lemon zest hand sanitizer is capable

of killing large percentage of bacteria.

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Definition of terms

Aloe Vera- This is a succulent plant species of the genus Aloe.

An evergreen perennial, it originates from the Arabian Peninsula but

grows wild in tropical climates around the world and is cultivated

for agricultural and medicinal uses. The species is also used for

decorative purposes and grows successfully indoors as a potted plant.

Lemon zest- This is made from the top layer of a lemon’s peel.

Hand sanitizer- This is also called hand antiseptic, hand rub,

or hand rub, agent applied to the hands for the purpose of removing

common pathogens (disease-causing organisms).1,2 Hand sanitizers

typically come in foam, gel, or liquid form.

Staphylococcus aureus- This is a gram-positive, round-shaped

bacterium that is a member of the Firmicutes, and it is a member of

the normal flora of the body, frequently found in the nose,

respiratory tract, and on the skin.

Kirby Bauer- This is a test of the antibiotic sensitivity of

bacteria. It uses antibiotic discs to test the extent to which

bacteria are affected by those antibiotics.

Citrus- is a genus of flowering trees and shrubs in the rue

family, Rutaceae. Plants in the genus produce citrus fruits,

including important crops such as oranges, lemons, grapefruits,

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pomelos, and limes. The genus Citrus is native to South Asia, East

Asia, Southeast Asia, Melanesia, and Australia.

Flavonoids- are a group of plant metabolites thought to provide

health benefits through cell signaling pathways and antioxidant

effects. These molecules are found in a variety of fruits and

vegetables. Flavonoids are polyphenolic molecules containing 15

carbon atoms and are soluble in water.

Zest- is a food ingredient that is prepared by scraping or

cutting from the outer, colorful skin of unwaxed citrus fruits such

as lemon, orange, citron, and lime. Zest is used to add flavor to

foods. The flavedo and white pith (albedo) of a citrus fruit together

makes up its peel.

Antibacterial- anything that destroys bacteria or suppresses

their growth or their ability to reproduce.

Bacteria- are a type of biological cell. They constitute a large

domain of prokaryotic microorganisms. Typically a few micrometers in

length, bacteria have a number of shapes, ranging from spheres to

rods and spirals. Bacteria were among the first life forms to appear

on Earth, and are present in most of its habitats.

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CHAPTER II

REVIEW OF RELATED LITERATURE

This chapter discusses certain articles pertaining to different

literature and studies.

Aloe Vera has been used in traditional medicine for thousands of

years to cure a variety of diseases. It has multiple properties that

are most effective treatment of many skin conditions, hair and also in

health conditions. The extract of leaf skins and flowers of Aloe Vera

have beneficial effects on human health and its extract possible

antioxidant and antimycoplasmic activities. The gel contains 99.3% of

water and the remaining 0.7% is made up of solids with carbohydrates

(Foster, 1999) and has been known to deliver excellent results when

applied to burns and wounds. Concentrated extracts of Aloe Vera leaves

are used laxatives and as hemorrhoid treatment. According to World

Health Organization (WHO), medicinal plants would be the best source

for obtaining a variety of drugs.

(David R. Macinga, 2013) Hand sanitizers reduce the microbial load

on hands without the need for soap and water. They are an important

tool to help reduce the spread of illness-causing microorganisms and

are useful as an adjunct to hand-washing. In settings such as health

care environments, alcohol-based hand sanitizers are the preferred and

recommended method of hand hygiene when the hands are not visibly

soiled.1 In addition, the use of alcohol-based hand sanitizers has

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become an international standard for proper hand hygiene and is strongly

endorsed by the Centers for Disease Control (CDC), the Association for

Professionals in Infection Control and Epidemiology (APIC), the Society

for Healthcare Epidemiology of America (SHEA), the Canadian Public

Health Agency, the World Health Organization (WHO), and others.

The use of hand sanitizers is expanding worldwide, in part as a

result of efforts by the WHO in its Patient Safety Initiative, which

now covers more than 85% of the world’s population.3 In fact, in 2007,

USA Today included one hand sanitizer in its list of 25 inventions that

have changed modern life during the past 25 years, along with the cell

phone, laptop computer and the iPod

In the United States, hand sanitizers fall under the purview of

the US Food and Drug Administration (FDA) and are regulated as drugs.

Current regulations allow hand sanitizer manufacturers to make data-

supported antibacterial- related claims such as “kills 99.99% of germs.”

Additional claims include, “reduces bacteria that potentially can cause

disease” and “decreases bacteria on the skin.”

Lemon (Citrus lemon) is a common citrus fruit, alongside

grapefruits, limes, and oranges. While the pulp and juice are used the

most, the peel tends to be discarded. However, studies have determined

that lemon peel also known as lemon zest. Zest is the outermost rind

of citrus fruits (usually lemons and oranges). It is valued for the

strong citrus flavor it imparts to food, thanks to an abundance of

 10 | P A G E

perfume, aromatic oils in the rind. It is full of bioactive compounds

that may provide numerous health benefits.

Lemon zest has high nutritional value due to having a high amount

of fiber and vitamin C, providing 9% of the DV in only 1 tablespoon.

Lemon zest offers several antioxidants, including D-limonene and

vitamin C, that protects your immune system and reduce your risk of

disease. Lemon peel may offer antimicrobial and antifungal effects —

even against antibiotic-resistant strains.

Flavonoids, vitamin C, and pectin in lemon peel may promote heart

health by lowering blood cholesterol levels and other risk factors for

heart disease. Lemon peel has various applications as a household

cleaner or beauty product. Lemon peel has no reported side effects and

is recognized by the FDA as safe for human consumption. Although lemon

peel normally gets thrown away, research shows that it possesses

numerous health benefits. Its fiber, vitamin, and antioxidant contents

may support oral, immune, and heart health. It may even have several

anticancer properties (Ariane Lang, 2019).

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CHAPTER III

RESEARCH METHODOLOGY

This chapter presents the methods and procedures of making Aloe

Vera gel and lemon zest as hand sanitizer. This chapter includes

research design, research locale, sample and sampling procedure, and

statistical designs.

Research Design

Experimental method is use to collect data. This study use Aloe

Vera gel and lemon zest which is expert of killing bacteria that

carrying different ailment or diseases by making it as a hand sanitizer.

In the experimental method, the hypothesis undergoes an

investigation that scientifically tested. When the experiment is

organized the independent variable which is the cause being manipulated

and the dependent variable which is the effect is being and extraneous

variables are controlled. When conducting this method, the experiments

should be objective. The perspective and thoughts of the people who

experiment will affect the results of study. This will make the data

more convincing and reliable.

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Materials and Instrument

Aloe Vera Gel

Aloe Vera is a succulent plant kind of the genus Aloe. An

evergreen perennial, The Arabian Peninsula is its origin but grows in

tropical climates around the world and is cultured for agricultural

and medicinal uses. In this study, the antimicrobial efficacy of Aloe

Vera gel is used to kill the bacteria.

Lemon Zest

Lemon zest is the upper layer of a lemon’s skin. It

contains the fruit’s oils and adds a bright, citrus flavor to a dish

or recipe. In this study, the ability to moisturize the hands is used

to avoid dryness.

Glycerin

Glycerin is a pure polyol compound. It is a colorless, odorless,

viscous liquid that is sweet-tasting and non-toxic. The glycerol

backbone is found in all lipids known as triglycerides. It is commonly

used in the food industry as a sweetener and humectant and in

pharmaceutical formulations. In this study, it makes the hand sanitizer

more viscous.
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Staphylococcus Aureus

Staphylococcus aureus- gram-positive, round-shaped bacterium that

is a member of the Firmicutes, and it is a usual member of the microbiota

of the body, frequently found in the upper respiratory tract and on the

skin. In this study, it is the bacteria that are used in testing.

Petri Dish

Petri dish- named after the German bacteriologist Julius Richard

Petri, is a shallow cylindrical glass or plastic lidded dish that

biologists use to culture cells – such as bacteria – or small mosses.

Müller-Hinton agar

It is a microbiological growth medium that is commonly used

for antibiotic susceptibility testing. It is also used to isolate and

maintain Neisseria and Moraxella species.

Forceps

Forceps are a handheld, hinged instrument used for grasping and

holding objects.

Alcohol Lamp

Alcohol lamp is a piece of laboratory equipment used to produce an

open flame. It can be made from brass, glass, stainless steel or

aluminum.
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Spray Bottle

Spray bottle is a bottle that can squirt, spray or mist fluids.

The researcher used it as a container to store their product.

Procedure

Phytochemical screening

It refers to the extraction, screening and identification of the

medicinally active substances found in plants. Some of the bioactive

substances that can be derived from plants are flavonoids, alkaloids,

carotenoids, tannin, antioxidants phenolic compounds and others.

The disk diffusion test, or agar diffusion test, or Kirby–Bauer

test (disc-diffusion antibiotic susceptibility test, disc-diffusion

antibiotic sensitivity test, KB test),

It is a test of the antibiotic sensitivity of a bacteria. It

uses antibiotic discs to test the extent to which bacteria are

affected by those antibiotics. In this test, wafers containing

antibiotics are placed on an agar plate where bacteria have been

placed, and the plate is left to incubate in 24 hours. If an

antibiotic stops the bacteria from growing or kills the bacteria,

there will be an area around the wafer where the bacteria have not

grown enough to be visible. This is called a zone of inhibition.

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The size of this zone depends on many factors, one being how

effective the antibiotic is at stopping the growth of the bacterium.

Another factor that will influence the size of a zone is the

diffusion of the antibiotic within the agar medium and varies based

on the molecular configuration of the antibiotic. Once the zone

diameter is measured it must be compared to a database of zone

standards to determine if the bacterium being studied is susceptible,

moderately susceptible or resistant to the antibiotic in question.

A pure bacterial culture is suspended in buffer, standardized to

turbidity and swabbed uniformly across a culture plate. A filter-

paper disk, impregnated with the compound to be tested, is then

placed on the surface of the agar. The compound diffuses from the

filter paper into the agar. The concentration of the compound will be

highest next to the disk, and will decrease as distance from the disk

increases. If the compound is effective against bacteria at a certain

concentration, no colonies will grow where the concentration in the

agar is greater than or equal to the effective concentration. This is

the zone of inhibition. This along with the rate of antibiotic

diffusion are used to estimate the bacteria's susceptibility to that

particular antibiotic. In general, larger zones correlate with

smaller minimum inhibitory concentration (MIC) of antibiotic for that

bacteria. Inhibition produced by the test is compared with that

produced by known concentration of a reference compound. This

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information can be used to choose appropriate antibiotics to combat a

particular infection.

Research Locale
This study was conducted on Wesleyan University-Philippines and Nueva

Ecija University of Science and Technology University which is

located in Cabanatuan City, Nueva Ecija.

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CHAPTER IV
RESULTS AND DISCUSSION

This chapter presents the results and data gathered. The researchers

analyzed and described carefully the results that have been taken

from the done experimentation.

The extraction of plants was successfully conducted and follows

the Kirby Bauer method and the incubation process that lasted for 24

hours.

Table 1 shows the phytochemical screening result of active

substances from aloe vera plant and lemon zest.

PHYTOCHEMICAL SCREENING RESULTS OF ALOE VERA AND LEMON ZEST PLANT

Analysis Result Positive Result

Greenish-black

Tannins - precipitate

Phlobatannins - Red precipitate

Saponins + Stable froth

Flavonoids - Pale yellow

precipitate

Coumarins - Yellow precipitate

Alkaloids - Flesh precipitate or

turbidity
 18 | P A G E

Terpenoids + Reddish-brown

coloration at the

interface

Cardiac Glycosides - Reddish-brown ring at

the interface

Efficacy of Aloe Vera Gel and Lemon Zest as Anti-Bacterial Hand

Sanitizer in Inhibiting the Growth of Staphylococcus aureus

Table 2 show all the trials that are done by the disc diffusion

method and it resulted 0. The formulation of the researchers is not

effective in killing the Staphylococcus aureus.

Table 2. Concentration of Aloe Vera Gel and Lemon Zest Hand

Sanitizer
TREATMENT NO. 1 MEASUREMENT OF ZONES OF
INHIBITION (ml)
CONCENTRATION 30ml Aloe Vera gel, 10ml Lemon
zest, 10ml Glycerin
TRIAL #1 0

TRIAL #2 0

TRIAL #3 0

TRIAL #4 0

TRIAL #5 0

MEAN ZONE 0
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As shown in Table 2, the researcher came up with a result of zero (0)

for the efficacy of hand sanitizer in all trials. The reason was because

of the insufficient phytochemical load of aloe vera plant and lemon

zest. It would be better if the researchers extracted more out of the

substances.
 20 | P A G E

CHAPTER V

SUMMARY, CONCLUSION AND RECOMMENDATIONS

This chapter presents the summary of findings, conclusions and

recommendations from the thorough study and analysis of data.

Summary

The focus of the study was to determine the efficacy of aloe vera

and lemon zest in preventing the growth of bacteria Staphylococcus

Aureus that is commonly found in hands, which can cause any skin

diseases or infections. If the product proves it effectiveness, it can

be the possible solution for the existing problem by making it as a

hand sanitizer.

This study is experimental research because this method is the

proper way of testing the antibacterial properties of the product using

a laboratory testing and to interpret correctly if the test bacteria

are resistant to the hand sanitizer.

Specifically, this research answered the questions of the efficacy

of aloe vera gel and lemon zest as antibacterial hand sanitizer in terms

of inhibiting the growth of Staphylococcus aureus.

As a result, the efficacy of aloe vera and lemon zest as an anti-

bacterial sanitizer resulted to negative or zero mean in all trial

concentration or simply not effective in killing the staphylococcus

 21 | P A G E

aureus. The reason is because of the insufficient phytochemical load of

aloe vera and lemon zest.

Conclusions

After the whole process of testing, the researchers have arrived

into the following conclusions:

1. The Organic Liquid Hand Sanitizer does not have the ability to

prevent the growth of Staphylococcus aureus.

2. The researchers Organic Hand Sanitizer is maybe more suitable

to kill other bacteria that are usually found in hands.

Recommendations

With regards of the findings of this study, the researchers would

like to forward the following recommendations:

1. The future researchers may test the current Organic Hand Sanitizer

in other bacteria like E. coli to address its flaws such as the

antibacterial effectiveness.

2. The future researchers may try to use other antibiotics to improve

its antibacterial effect. They may also try to separate extracts

in testing to see effectiveness of each formulation.

3. The future researchers may add or remove some ingredients to make

it more effective. They may also try to use different genus of

Aloe to test if it is more effective.

 22 | P A G E

References

Joe Leech 2017. What are the benefits of aloe vera?

Ariane Lang, 2019. 9 benefits and uses of lemon peel
David R. Macinga, 2013. Hand Sanitizer Activities: A Review
Foster, 1999. Evaluation of the nutritional and metabolic effects of
aloe vera
Aloe Vera: A short review
Evaluation of clinical effectiveness of aloe vera- a review

Websites
https://www.facebook.com/1739511626319537/posts/what-are-the-
benefits-of-aloe-veralast-reviewed-sun-28-may-2017by-joe-leech-
tabl/2288562771414417/
https://www.healthline.com/nutrition/lemon-peel
https://www.cosmeticsandtoiletries.com/formulating/function/anti
microbial/premium-hand-sanitizer-actives-a-review-
216615931.html?fbclid=IwAR2a2CDk_b2_0nd36UulWy5HNG5jWcwbXyQ2VIqz
az5Ih7Yu8DudCp0djlg
https://www.ncbi.nlm.nih.gov/books/NBK92765/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2763764/?fbclid=IwA
R3uvdwCcd5AI3WHRT-bYwRP22PJTyQNDguIX4VIZfBc5lRiGzcn0UIWXN4
http://www.jpccr.eu/Evaluation-of-clinical-effectiveness-of-
Aloe-vera-a-
review,74577,0,2.html?fbclid=IwAR1pVdH91IucMv9W_prDxie5L5EY_WGvq
z--z70S9iMx2gaIX1P-r2KTyjU
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Appendices

Materials and Instruments

Staphylococcus Aureus Molar Agar and Glycerin Petri dish

Beaker, foil and

Autoclave Sterilizer
phytochemical substances of Test tubes and tissue
aloe vera and lemon zest
 24 | P A G E

Research Locale

NEUST Wesleyan University- Philippines

Preparation of disc diffusion test

Zone of Inhibition
 25 | P A G E

CURRICULUM VITAE

CHRISTIAN WILL LINGAD ESTELLA

Bongabon, Nueva Ecija

Personal Data
Date of birth: - March 18, 2002
Place of Birth: - Premiere Medical Center,
Cabanatuan City
Age: - 17
Civil Status: - Single
Sex: - Male
Religion: - Roman Catholic
Father’s name: - Wiliiam E. Estella
Mother’s name: - Cecille L. Estella

Educational Background
Secondary - Al Taj International School
- Riyadh, Saudi Arabia
Elementary - Al Taj International School
- Riyadh, Saudi Arabia
Pre-Elementary - Al Taj International School
- Riyadh, Saudi Arabia
 26 | P A G E

CURRICULUM VITAE

MARRY JHOY ADELANTE DIMALANTA

San Leonardo, Nueva Ecija

Personal Data
Date of birth: - July 15, 2002
Place of Birth: - Castellano, San leonardo
Age: - 17
Civil Status: - Single
Sex: - Female
Religion: - Roman Catholic
Father’s name: - Elmar D. Dimalanta
Mother’s name: - Violeta A. Dimalanta

Educational Background
Secondary - Divina Pastora College
- Magpalo St. Gapan, Nueva Ecija
Elementary - Castellano Elementary School
- Castellano, San leonardo,
Nueva Ecija
Pre-Elementary - Castellano Elementary School
- Castellano, San leonardo,
Nueva Ecija
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CURRICULUM VITAE

MARK STEPHEN FLORES DELA CRUZ

Rizal, Nueva Ecija

Personal Data
Date of birth: - June 13, 2002
Place of Birth: - Premiere Medical Center,
Cabanatuan City
Age: - 17
Civil Status: - Single
Sex: - Male
Religion: - Born Again
Father’s name: - Ferdinand D. Dela Cruz
Mother’s name: - Sharon Rose F. Dela Cruz

Educational Background
Secondary - Bicos National High School
- Bicos, Rizal, Nueva Ecija
Elementary - Bicos Elementary School
- Bicos, Rizal, Nueva Ecija
Pre-Elementary - Charles Angel Montessori
School
- Llanera, Nueva Ecija
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CURRICULUM VITAE

MA. JURLET PANGILINAN DOLORMENTE

Gapan City, Nueva Ecija

Personal Data
Date of birth: - August 14, 2002
Place of Birth: - Gonzales Hospital,
San Leonardo
Age: - 17
Civil Status: - Single
Sex: - Female
Religion: - Roman Catholic
Father’s name: - Reynaldo D. Dolormente
Mother’s name: - Jennifer D. Dolormente

Educational Background
Secondary - Village Montessori School
- Gapan City, Nueva Ecija
Elementary - Village Montessori School
- Gapan City, Nueva Ecija
Pre-Elementary - Village Montessori School
- Gapan City, Nueva Ecija
 29 | P A G E

CURRICULUM VITAE

EUNICE BANTING JAVATE

Cabanatuan City, Nueva Ecija

Personal Data
Date of birth: - April 20, 2002
Place of Birth: - Good Samaritan Hospital,
Cabanatuan City
Age: - 17
Civil Status: - Single
Sex: - Female
Religion: - Catholic
Father’s name: - Rommel P. Javate
Mother’s name: - Emelita Sonia B. Javate

Educational Background
Secondary - Nueva Ecija High School
- Cabanatuan City, Nueva Ecija
Elementary - F.A Reyes Memorial
Elementary School
- Barrera, Cabanatuan City,
Nueva Ecija
Pre-Elementary - F.A Reyes Memorial
Elementary School
 30 | P A G E

CURRICULUM VITAE

ALEXANDRIA JAYE CADATAL REYES

Cabanatuan City, Nueva Ecija

Personal Data
Date of birth: - October 31, 2001
Place of Birth: - Premiere Medical Center,
Cabanatuan City
Age: - 18
Civil Status: - Single
Sex: - Female
Religion: - Born Again
Father’s name: - Jayson D. Reyes
Mother’s name: - Richellyn C. Reyes

Educational Background
Secondary - College of the Immaculate
Concepcion
- Cabanatuan City, Nueva Ecija
Elementary - Christian Faith Montessori
- Cabanatuan City, Nueva Ecija
Pre-Elementary - Christian Faith Montessori
- Cabanatuan City, Nueva Ecija
 31 | P A G E

CURRICULUM VITAE

NiCOLE P REYES
Aliaga, Nueva Ecija

Personal Data
Date of birth: - January 1, 2002
Place of Birth: - PJG Hospital, Cabanatuan City
Age: - 17
Civil Status: - Single
Sex: - Female
Religion: - Catholic
Father’s name: - Eduardo S. Reyes
Mother’s name: - Analiza P. Reyes

Educational Background
Secondary - Marciano Del Rosario Memorial
National High school
- Cabanatuan City, Nueva Ecija
Elementary - San juan Elementary School
- Aliaga, Nueva Ecija
Pre-Elementary - San juan Elementary School
- Aliaga, Nueva Ecija
 32 | P A G E

CURRICULUM VITAE

RUSTYN CHUA PADILLA

Cabanatuan City, Nueva Ecija

Personal Data
Date of birth: - September 16, 2001
Place of Birth: - Cabanatuan City, Nueva Ecija
Age: - 18
Civil Status: - Single
Sex: - Male
Religion: - Catholic
Father’s name: - Ferdie M. Padilla
Mother’s name: - Amygdhalia C. Padilla

Educational Background
Secondary - Nueva Ecija University of
Science and Technology
- Cabanatuan City, Nueva Ecija
Elementary - Cabanatuan East Elementary
School
- Cabanatuan City, Nueva Ecija
Pre-Elementary - Mabini Day care center
- Cabanatuan City, Nueva Ecija
 33 | P A G E

CURRICULUM VITAE

KARYLL ORLANDA SALONGA

San Antonio, Nueva Ecija

Personal Data
Date of birth: - July 27, 2001
Place of Birth: - San Antonio, Nueva Ecija
Age: - 18
Civil Status: - Single
Sex: - Female
Religion: - Roman Catholic
Father’s name: - Ricardo E. Salonga
Mother’s name: - Flordeliza O. Salonga

Educational Background
Secondary - Sta. Isabel Montessori (N.E) Inc.
- San Antonio, Nueva Ecija
Elementary - Sta. Isabel Montessori (N.E) Inc.
- San Antonio, Nueva Ecija
Pre-Elementary - Sta. Isabel Montessori (N.E) Inc.
- San Antonio, Nueva Ecija