NAME : MANIKA SINGH AGE:: 40 SEX: f DATE: 31/01/2025
REF. BY: dr. NIBEDITA CHETTARJEE EXAMINATION OF PATHOLOGICAL
TEST
Estimation of Quantitative 25 OH Vitamin D total by direct enzyme – linked immunosorbent
assay (ELISA) in Serum
INVESTIGATION RESULT BIOLOGICAL REFERENCE INTERVAL
VITAMIN D (TOTAL) – 25 OH, 17.46 ng/ml Vitamin D deficiency 5-20
SERUM BY ELISA Suboptimal Vitamin D Provision 20-30
Optimal Vitamin D level 30-50
Upper norm 50-70
Over dose but not toxic 70-15
Vitamin D intoxication >150
NOTE : Our Vitamin D assays is standardized to be in alignment with the ID-LC/MS/MS 25(OH) vitamin
D Reference method Procedure (RMP), the reference procedure for the Vitamin D Standardization
Program (VDSP). Vitamin D increasing in vitamin D intoxication. Vitamin D deficiency is a caused of
secondary hyperparathyroidism and diseases related to impaired Bone Metabolism like Rickets,
Oesteoporosis, Osteomalacia. Vitamin D3 (cholecalciferol) and Vitamin D2 (ergocalciferol) are the most
abundant Vitamin D in nature. Vitamin D3 is synthesized in the skin from 7-dehydrocholesterol in
response to UV-B rays in sunlight. The best nutrition sources of D3 are oily fish primary salmon and
mackerel. Vitamin D2's nutrition sources are from some vegetables, yeast and fungi. Vitamin D (D3 and
D2) is hydroxylated to 25-hydroxyvitamin D by an enzyme in the liver. The measurement of total 25-OH
vitamin Dconcentration in the serum or plasma is the best indicator of vitamin D nutritional status. The
optimal level of 25-OH Vitamin D is subject to some debate, but >32 ng/mL (> 80 nmol/L) is accepted as
sufficient for bone health. Vitamin D insufficient and deficiency are a recent growing public health
problem. Several research studies have identified widespread vitamin D insuficiency in apparent healthy
populations world-wide. Vitamin D deficiency is commonly treated with vitamin D2 or D3 medications
in doses ranging from 50,000 IU/month to 50,000 IU/week. Fortified foods and nutrition supplements
may contain either form. To ensure accurate assessment of the patient's vitamin D status the total 25-
OH vitamin D2 and D3. Recent studies have indentified an inactive 3-epimer of 25-OH vitamin D, which
has been detected in both infants and adults. It is important that an assay does not measure the
inactive 3-epi compound and only measure 25-OH vitamin D2 and D3 equally. More recently several
studies have suggested that Vitamin D insufficiency is associated with an increasing risk of many chronic
diseases including cardiovascular disease, cancer, infectious diseases and autoimmune diseases.
Comment : Please correlate with the clinical condition.
NAME : MAHUYA SARKAR AGE:: 53 SEX: f DATE: 01/02/2025
REF. BY: dr. SOMNATH DE EXAMINATION OF PATHOLOGICAL TEST
Estimation of Quantitative 25 OH Vitamin D total by direct enzyme – linked immunosorbent
assay (ELISA) in Serum
INVESTIGATION RESULT BIOLOGICAL REFERENCE INTERVAL
VITAMIN D (TOTAL) – 25 OH, 23.63 ng/ml Vitamin D deficiency 5-20
SERUM BY ELISA Suboptimal Vitamin D Provision 20-30
Optimal Vitamin D level 30-50
Upper norm 50-70
Over dose but not toxic 70-15
Vitamin D intoxication >150
NOTE : Our Vitamin D assays is standardized to be in alignment with the ID-LC/MS/MS 25(OH) vitamin
D Reference method Procedure (RMP), the reference procedure for the Vitamin D Standardization
Program (VDSP). Vitamin D increasing in vitamin D intoxication. Vitamin D deficiency is a caused of
secondary hyperparathyroidism and diseases related to impaired Bone Metabolism like Rickets,
Oesteoporosis, Osteomalacia. Vitamin D3 (cholecalciferol) and Vitamin D2 (ergocalciferol) are the most
abundant Vitamin D in nature. Vitamin D3 is synthesized in the skin from 7-dehydrocholesterol in
response to UV-B rays in sunlight. The best nutrition sources of D3 are oily fish primary salmon and
mackerel. Vitamin D2's nutrition sources are from some vegetables, yeast and fungi. Vitamin D (D3 and
D2) is hydroxylated to 25-hydroxyvitamin D by an enzyme in the liver. The measurement of total 25-OH
vitamin Dconcentration in the serum or plasma is the best indicator of vitamin D nutritional status. The
optimal level of 25-OH Vitamin D is subject to some debate, but >32 ng/mL (> 80 nmol/L) is accepted as
sufficient for bone health. Vitamin D insufficient and deficiency are a recent growing public health
problem. Several research studies have identified widespread vitamin D insuficiency in apparent healthy
populations world-wide. Vitamin D deficiency is commonly treated with vitamin D2 or D3 medications
in doses ranging from 50,000 IU/month to 50,000 IU/week. Fortified foods and nutrition supplements
may contain either form. To ensure accurate assessment of the patient's vitamin D status the total 25-
OH vitamin D2 and D3. Recent studies have indentified an inactive 3-epimer of 25-OH vitamin D, which
has been detected in both infants and adults. It is important that an assay does not measure the
inactive 3-epi compound and only measure 25-OH vitamin D2 and D3 equally. More recently several
studies have suggested that Vitamin D insufficiency is associated with an increasing risk of many chronic
diseases including cardiovascular disease, cancer, infectious diseases and autoimmune diseases.
Comment : Please correlate with the clinical condition.
NAME : MANASHI KARMAKAR AGE:: 40 SEX: f DATE: 01/02/2025
REF. BY: dr. SUKALYAN GHOSH EXAMINATION OF PATHOLOGICAL
TEST
Estimation of Quantitative 25 OH Vitamin D total by direct enzyme – linked immunosorbent
assay (ELISA) in Serum
INVESTIGATION RESULT BIOLOGICAL REFERENCE INTERVAL
VITAMIN D (TOTAL) – 25 OH, 07.58 ng/ml Vitamin D deficiency 5-20
SERUM BY ELISA Suboptimal Vitamin D Provision 20-30
Optimal Vitamin D level 30-50
Upper norm 50-70
Over dose but not toxic 70-15
Vitamin D intoxication >150
NOTE : Our Vitamin D assays is standardized to be in alignment with the ID-LC/MS/MS 25(OH) vitamin
D Reference method Procedure (RMP), the reference procedure for the Vitamin D Standardization
Program (VDSP). Vitamin D increasing in vitamin D intoxication. Vitamin D deficiency is a caused of
secondary hyperparathyroidism and diseases related to impaired Bone Metabolism like Rickets,
Oesteoporosis, Osteomalacia. Vitamin D3 (cholecalciferol) and Vitamin D2 (ergocalciferol) are the most
abundant Vitamin D in nature. Vitamin D3 is synthesized in the skin from 7-dehydrocholesterol in
response to UV-B rays in sunlight. The best nutrition sources of D3 are oily fish primary salmon and
mackerel. Vitamin D2's nutrition sources are from some vegetables, yeast and fungi. Vitamin D (D3 and
D2) is hydroxylated to 25-hydroxyvitamin D by an enzyme in the liver. The measurement of total 25-OH
vitamin Dconcentration in the serum or plasma is the best indicator of vitamin D nutritional status. The
optimal level of 25-OH Vitamin D is subject to some debate, but >32 ng/mL (> 80 nmol/L) is accepted as
sufficient for bone health. Vitamin D insufficient and deficiency are a recent growing public health
problem. Several research studies have identified widespread vitamin D insuficiency in apparent healthy
populations world-wide. Vitamin D deficiency is commonly treated with vitamin D2 or D3 medications
in doses ranging from 50,000 IU/month to 50,000 IU/week. Fortified foods and nutrition supplements
may contain either form. To ensure accurate assessment of the patient's vitamin D status the total 25-
OH vitamin D2 and D3. Recent studies have indentified an inactive 3-epimer of 25-OH vitamin D, which
has been detected in both infants and adults. It is important that an assay does not measure the
inactive 3-epi compound and only measure 25-OH vitamin D2 and D3 equally. More recently several
studies have suggested that Vitamin D insufficiency is associated with an increasing risk of many chronic
diseases including cardiovascular disease, cancer, infectious diseases and autoimmune diseases.
Comment : Please correlate with the clinical condition.
NAME : SUNNY ROY AGE:: 19 SEX: f DATE: 01/02/2025
REF. BY: dr. SELF EXAMINATION OF PATHOLOGICAL TEST
Estimation of Quantitative 25 OH Vitamin D total by direct enzyme – linked immunosorbent
assay (ELISA) in Serum
INVESTIGATION RESULT BIOLOGICAL REFERENCE INTERVAL
VITAMIN D (TOTAL) – 25 OH, 17.34 ng/ml Vitamin D deficiency 5-20
SERUM BY ELISA Suboptimal Vitamin D Provision 20-30
Optimal Vitamin D level 30-50
Upper norm 50-70
Over dose but not toxic 70-15
Vitamin D intoxication >150
NOTE : Our Vitamin D assays is standardized to be in alignment with the ID-LC/MS/MS 25(OH) vitamin
D Reference method Procedure (RMP), the reference procedure for the Vitamin D Standardization
Program (VDSP). Vitamin D increasing in vitamin D intoxication. Vitamin D deficiency is a caused of
secondary hyperparathyroidism and diseases related to impaired Bone Metabolism like Rickets,
Oesteoporosis, Osteomalacia. Vitamin D3 (cholecalciferol) and Vitamin D2 (ergocalciferol) are the most
abundant Vitamin D in nature. Vitamin D3 is synthesized in the skin from 7-dehydrocholesterol in
response to UV-B rays in sunlight. The best nutrition sources of D3 are oily fish primary salmon and
mackerel. Vitamin D2's nutrition sources are from some vegetables, yeast and fungi. Vitamin D (D3 and
D2) is hydroxylated to 25-hydroxyvitamin D by an enzyme in the liver. The measurement of total 25-OH
vitamin Dconcentration in the serum or plasma is the best indicator of vitamin D nutritional status. The
optimal level of 25-OH Vitamin D is subject to some debate, but >32 ng/mL (> 80 nmol/L) is accepted as
sufficient for bone health. Vitamin D insufficient and deficiency are a recent growing public health
problem. Several research studies have identified widespread vitamin D insuficiency in apparent healthy
populations world-wide. Vitamin D deficiency is commonly treated with vitamin D2 or D3 medications
in doses ranging from 50,000 IU/month to 50,000 IU/week. Fortified foods and nutrition supplements
may contain either form. To ensure accurate assessment of the patient's vitamin D status the total 25-
OH vitamin D2 and D3. Recent studies have indentified an inactive 3-epimer of 25-OH vitamin D, which
has been detected in both infants and adults. It is important that an assay does not measure the
inactive 3-epi compound and only measure 25-OH vitamin D2 and D3 equally. More recently several
studies have suggested that Vitamin D insufficiency is associated with an increasing risk of many chronic
diseases including cardiovascular disease, cancer, infectious diseases and autoimmune diseases.
Comment : Please correlate with the clinical condition.
