TRACE 1300 and TRACE 1310

Gas Chromatographs

Hardware Manual

PN 31715002 Revision F January 2016

© 2016 Thermo Fisher Scientific Inc. All rights reserved.

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Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
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The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.

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free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
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Release history:

First Edition, released March 2012 - “Original Instructions”

Second Edition September 2012; Third Edition October 2013; Fourth Edition January 2014, Fifth Edition
March 2015; Sixth Edition, January 2016

For Research Use Only. Not for use in diagnostic procedures.

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 Declaration
Manufacturer: Thermo Fisher Scientific

Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
• installation
• re-calibration
• changes and repairs

have been carried out by authorized personnel and if:

• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
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Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.

Thermo Fisher Scientific S.p.A.

Strada Rivoltana, 20090 Rodano - Milan - Italy — Tel: +39 02 950591 - Fax: +39 02 9505276

Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety
standards.

Safety
This device complies with the following safety standards according to Machinery Directive 2006/42/EC and Low
Voltage Directive 2006/95/EC.
• International Electrotechnical Commission (IEC): 61010-1:2001 (2nd edition) - 61010-2-010:2003
(2nd edition) - 61010-2-081:2001 (1st edition) + A1:(2003)
• National differences: CAN/CSA C22.2 No. 61010-1 (2nd Edition) - UL 61010-1 (2nd Edition)
• EuroNorm (EN): 61010-1:2001 (2nd Edition) – 61010-2-010:2004 (2nd Edition) - 61010-2-081:2002
(1st Edition)

Electromagnetic Compatibility
This device complies with the following regulations on Electromagnetic Compatibility (EMC) and Radio
Frequency Interference (RFI) according to directive 2004/108/EC:
• CISPR 11/EN 55011: Group 1 Class A
• IEC/EN 61326-1:2012 (

IMPORTANT: Class A equipment is intended for use in an industrial environment. In others

environments there may be potential difficulties in ensuring electromagnetic compatibility, due
to the conducted as well as radiated disturbances.
 FCC Compliance Statement

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

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Thermo Scientific Instruments
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Notice on the Susceptibility

to Electromagnetic Transmissions
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 C
Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Power Rating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Hydrogen Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
Using Hydrogen with TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . .xxiii
Hydrogen Connection Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Purchasing Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Properly Storing Hydrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Hydrogen Safety Codes, Standards and References . . . . . . . . . . . . . . . . . . xxvii
Hazardous Substances Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxix
Venting Toxic Gases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxix
Liquid Nitrogen Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxix
Carbon Dioxide Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxx

Chapter 1 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Positioning the TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Installing the External Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Installing the Injector and Detector Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installing an Injector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installing a Detector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Making the Gas Supply Plumbing Connections . . . . . . . . . . . . . . . . . . . . . . . . . 7
Testing for Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Connecting the Oven Cryogenic System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Oven Cryo System with Carbon Dioxide Connection . . . . . . . . . . . . . . . . . . 13
Oven Cryo System with Liquid Nitrogen Connection. . . . . . . . . . . . . . . . . . 16
Connecting the PTV/PTVBKF Cryogenic System . . . . . . . . . . . . . . . . . . . . . . 18
PTV/PTVBKF Cryo System with Carbon Dioxide Connection . . . . . . . . . . 18
PTV/PTVBKF Cryo System with Liquid Nitrogen Connection . . . . . . . . . . 21

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual ix

Contents

Coupling to a Mass Spectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Making the Duct to Couple with an ISQ Series, TSQ 8000 Series,
DSQ II, or ITQ Mass Spectrometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Making the Duct to Couple with a TSQ Quantum Mass
Spectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Making the Duct to Couple with a DFS, IRMS, or ICP-MS Mass
Spectrometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Setting Handshake Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Installing the Autosampler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Mounting an Autosampler on the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Connecting the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Connect the Autosampler to the GC System . . . . . . . . . . . . . . . . . . . . . . . . . 37
Setting Autosampler Handshake Parameters . . . . . . . . . . . . . . . . . . . . . . . . . 41
Installing the Data System Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Making Power Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Setting the LAN Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Making the LAN Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Configuring the Data System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Column Installation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Using the Correct Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Installing the Adapters for Encapsulated Graphite Ferrules . . . . . . . . . . . . . . 52
Installing the Column Rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Installing the Column the First Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Chapter 2 Performing Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65

Read Me First . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Maintenance Supplies and Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Cleaning Stainless Steel Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Maintenance Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Powering On the TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . . . . . 69
Shutting Down the TRACE 1300/TRACE 1310 . . . . . . . . . . . . . . . . . . . . . . . 71
Cleaning the Instrument Externally. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Replacing a Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Chapter 3 Performing Injectors Routine Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

Maintaining a Split/Splitless Injector (SSL) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Replacing the SSL Septum. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Cleaning or Replacing the SSL Glass Liner . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Replacing a SSL Broken Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Replacing the SSL Carrier and Split Lines Filters. . . . . . . . . . . . . . . . . . . . . . 88
Replacing the SSL Body Head O-Rings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

x TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 Contents

Maintaining a Split/Splitless Injector with Backflush (SSLBKF) . . . . . . . . . . . . 91

Replacing the SSLBKF Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Cleaning or Replacing the SSLBKF Glass Liner. . . . . . . . . . . . . . . . . . . . . . . 95
Replacing a SSLBKF Broken Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Replacing the SSLBKF Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . 99
Replacing the SSLBKF Body Head O-Rings . . . . . . . . . . . . . . . . . . . . . . . . 100
Maintaining a Gas Sampling Valve Injector (GSV) . . . . . . . . . . . . . . . . . . . . . 102
Connecting the Sample In and Out Lines . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Replacing the Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . . . . . . . 105
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Replacing the Sample Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL) . . . . . . . . 108
Replacing the Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Cleaning or Replacing the Glass Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Replacing the Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . . . . . . . 114
Replacing the Body Head O-Rings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Maintaining a Programmable Temperature Vaporizing Injector (PTV). . . . . . 117
Replacing the PTV Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Cleaning or Replacing the PTV Glass Liner . . . . . . . . . . . . . . . . . . . . . . . . 121
Replacing the PTV Broken Liner. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Replacing the PTV Carrier and Split Lines Filters . . . . . . . . . . . . . . . . . . . . 125
Maintaining a Programmable Temperature Vaporizing Injector with
Backflush (PTVBKF) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Replacing the PTVBKF Septum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Cleaning or Replacing the PTVBKF Glass Liner . . . . . . . . . . . . . . . . . . . . . 131
Replacing the PTVBKF Broken Liner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Replacing the PTVBKF Carrier and Split Lines Filters . . . . . . . . . . . . . . . . 135

Chapter 4 Performing Detectors Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . .137

Maintaining a Flame Ionization Detector (FID) . . . . . . . . . . . . . . . . . . . . . . . 138
Cleaning or Replacing the FID Collecting Electrode . . . . . . . . . . . . . . . . . . 141
Replacing the FID Ignition Glow-plug . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
Maintaining a Nitrogen Phosphorous Detector (NPD) . . . . . . . . . . . . . . . . . . 147
Replacing the NPD Thermionic Source . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
Cleaning or Replacing the NPD Collecting Electrode . . . . . . . . . . . . . . . . . 156
Maintaining a Thermal Conductivity Detector (TCD) . . . . . . . . . . . . . . . . . . 163
Bake-out Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
Measuring the Carrier Gas Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Shutting Down the TCD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Maintaining an Electron Capture Detector (ECD) . . . . . . . . . . . . . . . . . . . . . 166
Detector Chemical Contamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
Wipe Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Cleaning or Replacing the Collecting Electrode (Anode) . . . . . . . . . . . . . . . 169

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xi

Contents

Maintaining a Flame Photometric Detector (FPD) . . . . . . . . . . . . . . . . . . . . . 172

Installing the FPD Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176
Removing the FPD Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Cleaning or Replacing the FPD Jet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Cleaning or Replacing the FPD Interferential Filter . . . . . . . . . . . . . . . . . . 183
Replacing the FPD Ignition Glow-plug . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
Maintaining a Pulsed Discharge Detector (PDD) . . . . . . . . . . . . . . . . . . . . . . 193

Chapter 5 GC Main Frame Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195

Removing/Replacing the GC Top Cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
Removing/Replacing the GC Left Side Panel . . . . . . . . . . . . . . . . . . . . . . . . . 197
Removing/Replacing the GC Right Side Panel . . . . . . . . . . . . . . . . . . . . . . . . 199
Removing/Replacing the GC Back Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . 201
Removing/Replacing the GC Front Door Cover . . . . . . . . . . . . . . . . . . . . . . . 203
Removing/Replacing the Electronic Module . . . . . . . . . . . . . . . . . . . . . . . . . . 208
Replacing the Oven Heater Baffle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211
Replacing the Oven Heater Temperature Sensor . . . . . . . . . . . . . . . . . . . . . . . 215
Replacing the Oven Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 219
Replacing the Flap Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225

Chapter 6 Injectors Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .229

Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and
PTVBKF Injectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230
Removing/Replacing an Injector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231
Cleaning the SSL Injector Body. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 234
Cleaning the SSLBKF Injector Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 238
Cleaning the HeS-S/SL Injector Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242
Cleaning the PTV Injector Head Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . 246
Cleaning the PTVBKF Injector Head Assembly . . . . . . . . . . . . . . . . . . . . . . . 253

Chapter 7 Detectors Advanced Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .261

Removing/Replacing a Detector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 262
Measuring the FID Gas Flows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
Cleaning or Replacing the FID Jet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Measuring the NPD Gas Flows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 276
Cleaning or Replacing the NPD Jet. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285
Measuring the FPD Gas Flows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293
Cleaning or Replacing the FPD Mirror Metal Plug . . . . . . . . . . . . . . . . . . . . . 295
Cleaning or Replacing the FPD Filter-side Heat Shields . . . . . . . . . . . . . . . . . 300
Cleaning or Replacing the FPD Flame-side Heat Shields. . . . . . . . . . . . . . . . . 308
Replacing the FPD Photomultiplier Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . 317

Chapter 8 Installing Optional Kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .325

Installing the Oven Exhaust Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326

xii TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Contents

Installing the Merlin Microseal High Pressure Valve Kit . . . . . . . . . . . . . . . . . 327

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 327
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 327
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector . . . . . . 332
Installing the Packed Column Adapters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338
Installing the HS Adapter Kit on the SSL/SSLBKF Injector . . . . . . . . . . . . . . 344
Installing the Large Volume Splitless Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 354
Installing the Manual On/Off Valve for Single Gas Line . . . . . . . . . . . . . . . . . 357
Connecting a SSL/PTV Backflush System. . . . . . . . . . . . . . . . . . . . . . . . . . . . 360
Connecting a SSL/PTV Backflush System for High Temperature . . . . . . . . . . 367
Connecting a GSV Backflush System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 373
Installing the NoVent Microfluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 376
Connecting the NoVent Microfludics Module to the TRACE
1300/1310 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 376
Installing the Mounting Bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379
Preparing the NoVent Microfluidics Restrictor Tubing. . . . . . . . . . . . . . . . 381
Attaching the Ferrule and Nut to the GC Column . . . . . . . . . . . . . . . . . . . 384
Attaching the New Tubing to the Transfer Line . . . . . . . . . . . . . . . . . . . . . 385
Connecting the Capillaries to the Microfluidics Splitter . . . . . . . . . . . . . . . 390
Configuring the Post-Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391
Using the Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 394
Installing a FTIR Make-up Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 396
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector . . . . . . 400
Performing the Dual FPD Detector Configuration . . . . . . . . . . . . . . . . . . . . . 409

Chapter 9 Adding Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .421

Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module . . . . . . . . . . . . . 422
Adding a GSV Injector Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 425
Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module . . . . . . 431
Adding a NPD Detector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 435
Adding an Aux Temperature/Cryo Module. . . . . . . . . . . . . . . . . . . . . . . . . . . 443
Adding a Helium Saver Injector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 448
Installing the Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 448
Installing the Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 454
Checking for Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 456

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xiii
 Adding a PDD Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 458
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 458
Installing a PDD Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 459
Plumbing the Gas Lines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 463
Connecting the High Voltage and Pulses Cables . . . . . . . . . . . . . . . . . . . . . 465
Installing the Capillary Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 470
Installing the Packed Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 470
Testing for Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 470
Performing Initial Power Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 471
Adding a Generic Detector Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 472
Preliminary Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 472
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 472
Removing the GC Back Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 473
Assembling the GDI Electrical Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . 474
Replacing the Encapsulated Flow Restrictors . . . . . . . . . . . . . . . . . . . . . . . . 476
Installing and Connecting the GDI Electrical Interface . . . . . . . . . . . . . . . . 477
Connecting a GDI Interface to the TRACE 1310 Auxiliary Oven. . . . . . . . 481
Installing a GDI Mechanical Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 482
Connecting the Detector Gas Tubings to the Manifolds . . . . . . . . . . . . . . . 484
Connecting Heater and Signal Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 488
Restarting the GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 489
Performing the Third-party Detector Start-up and Optimization . . . . . . . . 489
Configuring and Setting GDI Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . 490
Adding an Analog Output Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 491

Chapter 10 Adding Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .495

Adding the Oven Cryo System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 496
Oven Cryo System Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 497
Installing the Oven Cryo System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 499
Adding the PTV and PTVBKF Cryo System. . . . . . . . . . . . . . . . . . . . . . . . . . 510
PTV/PTVBKF Cryo System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . 511
Installing the PTV/PTVBKF Cryo System . . . . . . . . . . . . . . . . . . . . . . . . . 513
Adding an Auxiliary Gas System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 521
Auxiliary Gas Module Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 522
Auxiliary Gas Interface Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 522
Preliminary Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 524
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 525
Installing the Auxiliary Gas Interface on the Left Wall of the Oven . . . . . . . 525
Installing the Auxiliary Gas Interface on the Right Wall of the Oven . . . . . 528
Installing the Auxiliary Gas Interface on the Oven for HRMS. . . . . . . . . . . 532
Installing and Connecting the Auxiliary Gas Module . . . . . . . . . . . . . . . . . 534
Adding the Hydrogen Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 542

Chapter 11 Upgrade Equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .549

Upgrading a TRACE 1300 to a TRACE 1310 . . . . . . . . . . . . . . . . . . . . . . . . 550
 Contents

Upgrading a Stand Alone TRACE 1300/TRACE 1310 to MS Version. . . . . . 556

Preliminary Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 556
Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 556
Coupling with the ISQ Series / TSQ 8000 Series Mass Spectrometer . . . . . 557
Coupling with the DSQ II Mass Spectrometer . . . . . . . . . . . . . . . . . . . . . . 561
Coupling with the ITQ Mass Spectrometer. . . . . . . . . . . . . . . . . . . . . . . . . 566
Coupling with the TSQ Quantum Mass Spectrometer . . . . . . . . . . . . . . . . 571
Updating HMI Software From USB Stick. . . . . . . . . . . . . . . . . . . . . . . . . . . . 577

Chapter 12 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .581

Investigating Power Supply Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 582
TRACE 1300/TRACE 1310 will not power-on . . . . . . . . . . . . . . . . . . . . . 582
Investigating Communication Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 582
Software is not communicating with the TRACE 1300/TRACE 1310 . . . . 582
TRACE 1300/TRACE 1310 does not start or is not ready . . . . . . . . . . . . . 582
Cannot download methods to the TRACE 1300/TRACE 1310 . . . . . . . . 582
Sample data are not acquired . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 583
GC is not communicating with the PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . 583
Autosampler is not communicating with the PC . . . . . . . . . . . . . . . . . . . . . 583
Investigating Sensitivity Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 583
Poor sensitivity or sudden loss in sensitivity . . . . . . . . . . . . . . . . . . . . . . . . . 583
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584
TRACE 1300 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584
TRACE 1310 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 584
Contacting Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 589

Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .591

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xv

Microsoft Office Word 2007.lnk

Preface
This manual contains detailed information about installing, maintaining, and
troubleshooting the TRACE 1300/TRACE 1310 gas chromatograph.

This manual is organized as follows:

Chapter 1, “Installation,” provides instructions for installing and connecting the TRACE
1300/TRACE 1310 system.

Chapter 2, “Performing Routine Maintenance,” provides instructions for performing routine

maintenance on the TRACE 1300 and TRACE 1310 modules.

Chapter 3, “Performing Injectors Routine Maintenance,” provides instructions for performing

routine maintenance on the TRACE 1300 and TRACE 1310 injector modules.

Chapter 4, “Performing Detectors Routine Maintenance,” provides instructions for

performing routine maintenance on the TRACE 1300 and TRACE 1310 detector modules.

Chapter 5, “GC Main Frame Advanced Maintenance,” describes TRACE 1300/TRACE

1310 components that do not require routine maintenance, but they need to be removed or
replaced.

Chapter 6, “Injectors Advanced Maintenance,” describes TRACE 1300/TRACE 1310

injector modules that do not require routine maintenance, but troubleshooting may indicate
they need to be cleaned or replaced.

Chapter 7, “Detectors Advanced Maintenance,” describes TRACE 1300/TRACE 1310

detector modules that do not require routine maintenance, but troubleshooting may indicate
they need to be cleaned or replaced.

Chapter 8, “Installing Optional Kits,” describes how to install the optional kits available for
the TRACE 1300/TRACE 1310.

Chapter 9, “Adding Modules,” describes how to install any added module that is available for
the TRACE 1300/TRACE 1310.

Chapter 10, “Adding Systems,” describes how to install any added system that is available for
the TRACE 1300/TRACE 1310. See the TRACE 1300 and TRACE 1310 Spare Parts Guide
for information about ordering the equipment in this chapter.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xvii
Preface
About Your System

Chapter 11, “Upgrade Equipment,” describes how to install any upgraded equipment that is
available for the TRACE 1300/TRACE 1310.

Chapter 12, “Troubleshooting,” describes the symptom, and the remedy for each known issue
with the TRACE 1300/TRACE 1310 gas chromatograph.

“Glossary,” contains definitions of terms used in this guide. It also includes abbreviations,
acronyms, metric prefixes, and symbols.

About Your System

Thermo Scientific systems provide high-caliber gas chromatography (GC) instrumentation.
Your TRACE 1300/TRACE 1310 GC system can be a stand-alone unit or coupled with other
instruments. GC represents a powerful analytical separation technique. Complex mixtures of
individual compounds can be injected into the GC, either manually or by using an
autosampler, and then separated the eluate for presentation to the detector. The detector
generates signals of the GC eluate and its components. These signals are then processed by a
Thermo Scientific Chromatography Data System for qualitative identification, as well as
accurate and precise quantification of the individual compounds present in the sample.

IMPORTANT Thermo Scientific systems optimize the separation and detection

capabilities of GC by providing high performance analytical capabilities for both research,
and routine applications. More information about the use of this system can be found in
related documentation sources, and by using the provided contact information.

WARNING Thermo Scientific systems operate safely and reliably under carefully
controlled environmental conditions. If the equipment is used in a manner not specified
by the manufacturer, the protections provided by the equipment might be impaired. If
you maintain a system outside the specifications listed in this guide, failures of many
types, including personal injury or death, might occur. The repair of instrument failures
caused by operation in a manner not specified by the manufacturer is specifically excluded
from the standard warranty and service contract coverage.

WARNING Operation of this system requires the use of chemical substances with
different hazard specifications. Before using any chemicals, read the hazard indications
and information reported in the Safety Sheet supplied by the manufacturer, referring to
the relevant CAS (Chemical Abstract Service) number.

Power Rating
TRACE 1300/TRACE 1310 gas chromatograph
• 120 Vac ±10%, 50/60 Hz, 2000 VA
• 230 Vac ±10%, 50/60 Hz, 2000 VA

Detailed instrument specifications are in the Product Specifications or Product Brochure.

xviii TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Preface
Contacting Us

Contacting Us
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.

Use http://www.thermoscientific.com address for products information.

Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to

contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer Support.

Related Documentation
In addition to this guide, Thermo Scientific provides the following documents for the
TRACE 1300 and TRACE 1310. These documents are also available on a
“Print-By-Request” basis.

TRACE 1300 and TRACE 1310 Document Set, PN 31715000

• TRACE 1300 and TRACE 1310 Preinstallation Requirements Guide, PN 31715001
• TRACE 1300 and TRACE 1310 Hardware Manual, PN 31715002
• TRACE 1300 and TRACE 1310 User Guide, PN 31715003
• TRACE 1300 and TRACE 1310 Spare Parts Guide, PN 31715004

Safety Alerts and Important Information

Make sure you follow the precautionary notices presented in this manual. The safety and
other special notices appear in boxes.

Special Notices
Notices includes the following:

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal
performance of the system.

Note Emphasizes important information about a task.

Tip Helpful information that can make a task easier.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xix
Preface
Safety Alerts and Important Information

Safety Symbols and Signal Words

All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury, instrument damage, or both. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION is intended to prevent improper actions
that might cause personal injury, instrument damage, or both. You can find the following
safety symbols on your instrument, or in this manual:

Symbol Descriptor

BIOHAZARD: Indicates that a biohazard will, could, or might occur.

BURN HAZARD: Alerts you to the presence of a hot surface that could or might
cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.

FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.

EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates

this risk could or might cause physical injury.
FLAMMABLE GAS HAZARD. Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
CLOTHING REQUIRED. Indicates that you should wear a work clothing when
performing a task or else physical injury could or might occur.
BOOTS REQUIRED. Indicates that you must wear boots when performing a
task or else physical injury could or might occur.

MATERIAL AND EYE HAZARD. Indicates you must wear eye protection when
performing a task.

HAND AND CHEMICAL HAZARD: Indicates that chemical damage or physical

injury could or might occur.
HARMFUL. Indicates that the presence of harmful material will, could, or
might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.

xx TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 Preface
Instrument Markings and Symbols

LIFTING HAZARD. Indicates that a physical injury could or might occur if two
or more people do not lift an object.
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for a
physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.

LASER HAZARD. Indicates that exposure to a laser beam will, could, or might
cause personal injury.
RADIOACTIVE HAZARD. Indicates that the presence of radioactive material
could or might occur.

For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard. In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.

Instrument Markings and Symbols

Table 1 explains the symbols used on Thermo Scientific instruments. Only a few of them are
used on the TRACE 1300/1310. See the asterisk.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
* Alternating Current
Both direct and alternating current

3 Three-phase alternating current

Earth (ground) terminal

Protective conductor terminal

Frame or chassis terminal

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xxi
Preface
Hydrogen Safety Precautions

Table 1. Instrument Marking and Symbols (Sheet 2 of 2)

Symbol Description
Equipotentiality

* On (Supply)

* Off (Supply)

Equipment protected throughout by DOUBLE INSULATION or

REINFORCED INSULATION (Equivalent to Class II of IEC 536)
* Instruction manual symbol affixed to product. Indicates that the user must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock

* Caution, hot surface

* Caution, biohazard

In-position of a bistable push control

Out-position of a bistable push control

* + - Jack socket

* Symbol in compliance to the Directive 2012/19/EU on Waste Electrical

and Electronic Equipment (WEEE) placed on the European market after
August, 13, 2005.

Hydrogen Safety Precautions

Hydrogen is a colorless, odorless, highly flammable gas with the molecular formula H2 and an
atomic weight of 1.00794, making it the lightest element. Hydrogen gas presents a hazard, as
it is combustible over a wide range of concentrations; at ambient temperature and pressure,
the range is from about 4 to 74.2% by volume.

Hydrogen has a flash point of -423 °F (-253 °C) and an auto-ignition temperature of
1040 °F (560 °C). It has a very low ignition energy and the highest burning velocity of any
gas. If hydrogen is allowed to expand rapidly from high pressure, it can self-ignite. Hydrogen
burns with a flame that can be invisible in bright light.

xxii TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Preface
Hydrogen Safety Precautions

WARNING - EXPLOSION HAZARD The use of hydrogen as a carrier gas is dangerous.

Hydrogen is potentially explosive and must be used with extreme care. Any use of
hydrogen gas must be reviewed by appropriate health and safety staff, and all installations
of hydrogen systems must be performed to applicable codes and standards. Thermo Fisher
Scientific assumes no liability for the improper use of hydrogen as a carrier gas.

Before you begin using hydrogen, conduct a risk assessment based on the quantity of
hydrogen to be used and the conditions of your laboratory. Ask yourself:
“What hydrogen hazards associated with this project are most likely to occur?”
“What hydrogen hazards associated with this project have the potential to result in the
worst consequences?”
• Try to reduce or eliminate the higher risks by using the proper ventilation to remove
hydrogen gas before an ignitable concentration can accumulate. Also consider purging the
hydrogen to further reduce hazards and ensure that anyone working with hydrogen has
basic hydrogen safety training.
• As with laboratory safety in general, be sure to wear safety glasses, laboratory coats, gloves,
and so on. Typically there are no specific requirements for gaseous hydrogen, other than
eye protection when working with a compressed gas. If working with liquid (cryogenic)
hydrogen, wear insulated gloves and protective shoes in addition to eye protection.
• Post “No Smoking” and “No Open Flames” signs to identify hydrogen sources and
cylinders. Maintain, inspect, and leak-test all hydrogen sources regularly.
• Clearly mark all hydrogen shutoff valves and label permanent hydrogen piping as such at
the supply or discharge point, and at regular intervals along its length. Where hydrogen
gas piping passes through a wall, be sure to label both sides of the wall.
• Have contingency plans in place should an incident occur.
• Ensure that site emergency response team, as well as the local fire department, knows the
location of all hydrogen storage tanks.

Using Hydrogen with TRACE 1300/TRACE 1310

The use of hydrogen as a carrier gas, or as fuel gas for certain flame detectors, requires strict
attention and compliance with special precautions due to the hazards involved.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xxiii
Preface
Hydrogen Safety Precautions

WARNING - EXPLOSION HAZARD Hydrogen is a dangerous gas that, when mixed with
air, could create an explosive mixture. The use of hydrogen as a carrier gas requires
extreme caution. Special precautions must be taken because of the risk of explosion. When
hydrogen is used as carrier gas the gas chromatograph must be equipped with a hydrogen
sensor.

Never use hydrogen as carrier gas in your TRACE 1300/TRACE 1310 system unless your
oven has a hydrogen sensor installed. Thermo Fisher Scientific FSEs are not authorized to
install or repair any instrument using hydrogen as a carrier gas unless the instrument is
equipped with the appropriate sensor.

If your oven does not have a hydrogen sensor already installed, contact your Thermo
Fisher Scientific sales representative. To comply with instrument safety requirements, a
Thermo Fisher Scientific FSE authorized service personnel should install the sensor into
your TRACE 1300/TRACE 1310.

Hydrogen is a dangerous gas, particularly in an enclosed area when it reaches a concentration

corresponding to its lower explosion level (4% in volume). An explosion hazard could develop
in the oven when hydrogen is used as a carrier gas in the case oven elements are not perfectly
connected to each other, or when the connection materials are worn out, broken, or otherwise
faulty.

Use the following safety precautions when using hydrogen:

• Ensure that all hydrogen cylinders comply with the safety requirements for proper use
and storage. Hydrogen cylinders and delivery systems must comply with local regulations.
• Make sure the gas supply is turned completely off when connecting hydrogen lines.
• Perform a leak test to ensure that the hydrogen lines are leak-tight before using the
instrument. Repeat this test to eliminate all leaks.
• Ensure your TRACE 1300/TRACE 1310 has a Thermo Scientific hydrogen sensor
installed for continuously monitoring the hydrogen level in the oven.

Hydrogen Connection Guidelines

Use the following guidelines to safely connect hydrogen to your system:
• Piping—Hydrogen must be delivered to equipment using appropriate piping and be
done in such a way as to pose essentially no hazard to end-users. Piping systems for the
delivery of hydrogen should be designed and installed by a person qualified by specific
training and experience with hydrogen piping systems.
Stainless steel is usually recommended because it is a safe, cost-effective material. Piping
of black iron or copper must not be used, as the pipe can become brittle with age.
Elastomeric/plastic tubing of various plastics and polymers should not be used, unless the

xxiv TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Preface
Hydrogen Safety Precautions

tubing is approved for use with hydrogen. If elastomeric/plastic tubing is used for
hydrogen gas delivery, the tubing should be tested for hydrogen permeability to minimize
leakage.
The hydrogen piping system must be flexible enough to endure routine thermal
expansion and contraction. The system should also include considerations for the most
severe condition of temperature and pressure expected during service. Piping and
supports must be able to withstand static loading introduced by such things as ice and
snow; and dynamic loading from high wind and earthquake.
Caution should be used if burying hydrogen piping. Proper controls should be used to
protect against damage and corrosion, and also to prevent Hydrogen from entering a
building if there is any leakage.
• Fittings—All fittings must be of the proper type approved or designed for use with
hydrogen gas. Use as few fittings as possible to minimize the potential for leaks. After
installation, ensure that leak testing is carried out prior to system use, and on a regular
basis.
There must be no PTFE tape or other things like plumber's putty used to enhance a seal, as
this actually is a detriment to a good seal. Ideally the best installation would use stainless
steel tubing with appropriate gas-tight fittings.
Welding is usually preferred for joints in hydrogen piping systems since welding provides
a better connection and reduces the potential for leaks compared to mechanical fittings.
Soft solder joints are not permitted for hydrogen systems (due to the low melting point of
soft solder and its potential for brittle failure at cryogenic temperatures). Brazed joints are
permitted, but such joints should be protected against the possibility of external fire.
Tubing connections should be clamped to barbed or press-fit type connections. Hose
clamps or jubilee clamps must not be used.
• Valves—All valves must be suitable for hydrogen service and for the specific operating
conditions. Valves, including regulators, must not be used for hydrogen, unless they are
designed and identified for such a use. Ball valves are often chosen because of their
superior leak tightness through the valve seat. Pneumatic operators are usually chosen for
remotely operated valves so that potential ignition sources (electricity) are remote from
the valve.
Manual shutoff valves should be provided near each point of use, within immediate reach.
If a hydrogen cylinder or hydrogen generation system is located within immediate reach,
a separate point-of-use shutoff valve is usually not necessary.
Line regulators that have their source away from the point of use should have a manual
shutoff valve near the point of use.
An emergency gas shutoff device in an accessible location outside the use area should be
provided in addition to the manual point-of-use valve in each educational and
instructional laboratory space that has a piped gas supply system.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xxv
Preface
Hydrogen Safety Precautions

If necessary, the piping system should have uninterruptible pressure relief. The pressure
relief system should be designed to provide a discharge rate sufficient to avoid further
pressure increase and should vent to a safe location outside or to a ventilation system
exhaust.

Purchasing Hydrogen
Use the following guidelines when purchasing hydrogen:
• Hydrogen Generator—Because it minimizes the amount of hydrogen present and reduces
the degree of hazard, a hydrogen generator (also called an electrolyzer) is the safest way to
purchase hydrogen in the quantity used in gas chromatography/mass spectroscopy
systems.
However, to minimize the degree of hazard, operate the hydrogen generator only in a
non-explosive environment because hydrogen buildup can be ignitable. Thus, your
ventilation system for the room or lab hood where the hydrogen generator operates must
maintain an air exchange rate at least two orders of magnitude greater than the maximum
hydrogen production rate of the hydrogen generator. Follow the manufacturers'
directions about proper use and maintenance of the regulator.
To prevent the possibility of releasing hydrogen, set the hydrogen generator to shut down
if:
− There is a loss of flow to the ventilation system
− A hydrogen detector alarms at 25% of the lower flammable limit of hydrogen in air.
Vent the oxygen exhausted by the electrolyzer to the outside as well.
• Hydrogen Cylinder—Hydrogen can be delivered in standard laboratory gas bottles or
cylinders. These cylinders have a limited amount of hydrogen in them and are a safe way
to transport and store hydrogen. Always secure, compressed hydrogen gas cylinders, like
all compressed gas cylinders, in an upright position, ideally with a non-combustible chain
or cable. If the cylinder falls over, the valve can fall off, causing the pressurized cylinder to
take off like a rocket, leading to the release of hydrogen and possibly an explosion, severe
injury, or death. Never crack a hydrogen cylinder valve to remove dust or dirt from
fittings prior to attaching a regulator, as there is a risk of self-ignition.

Properly Storing Hydrogen

Storing and handling compressed hydrogen gas and cryogenic liquid hydrogen present
potential health and safety hazards. Using proper storage and handling techniques is essential
to maintaining a safe work environment.

Use the following guidelines when storing hydrogen:

xxvi TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Preface
Hydrogen Safety Precautions

• Store spare hydrogen gas cylinders outside and away from doors, windows, building air
intake vents, structures, and vehicle routes. This precaution applies when the hydrogen is
or is not in use. Indoor storage of spare hydrogen cylinders has special requirements,
which are beyond the scope of this document. Documentation for each vessel should
include a description of the vessel, a list of available drawings or other documents, the
most recent inspection results, and the responsible person's name.
• Prevent spare cylinders from toppling by wrapping them with chains. The chains should
also be protected against corrosion and excessive heat.
• Separate spare hydrogen cylinders from oxidizing gases (such as oxygen) with a 5 ft.
(1.5 m) tall fire barrier with a half-hour fire rating or place the cylinders at least 20 ft.
(6 m) apart.
• When moving hydrogen cylinders:
− Remove the regulator and replace the cylinder valve cap before moving.
− Move cylinders on cylinder carts or with other appropriate transport devices.
− Never roll or drop a cylinder and never lift a cylinder by its protective cap.
• Bulk hydrogen systems include either gaseous or liquid hydrogen in fixed installations; in
some gas systems a semi-permanent trailer (tube trailer) can be used. Storage vessels for
compressed hydrogen gas or liquid hydrogen should be designed, constructed, tested, and
maintained in accordance with applicable codes and standards. Bulk hydrogen systems
represent a level of complexity again which is beyond the scope of this document;
however some general guidelines are provided.
• The bulk hydrogen storage system should not be located beneath electric power lines,
close to other flammable gases/liquids, or close to public areas. It should be readily
accessible to authorized personnel and delivery equipment, but protected from physical
damage or tampering.
• As liquid hydrogen systems also have a cryogenic hazard, additional safety considerations
for the use of cryogenic liquids might be necessary.

Hydrogen Safety Codes, Standards and References

The following list of safety codes, standards, and references is in no way an exhaustive list. In
fact, there may be federal, state, or local codes that apply to your specific location. Check with
all appropriate agencies with jurisdiction before installing or using a hydrogen system.
• Air Products Safetygram #4 Gaseous Hydrogen
• ANSI/AIAA standard for hydrogen safety guidelines is AIAA G-095-2004, Guide to
Safety of Hydrogen and Hydrogen Systems
• ASME B31.1, Power Piping Code
• ASME B31.3, Process Piping Code

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xxvii
Preface
Hydrogen Safety Precautions

• ASME B31.8, Gas Transmission and Distribution Systems

• BCGA Code Of Practice CP4 Industrial Gas Cylinder Manifolds and Gas Distribution
Pipework
• BCGA Code Of Practice CP33 The Bulk Storage of Gaseous Hydrogen at Users'
Premises
• CGA G-5, Hydrogen
• CGA G-5.4, Standard for Hydrogen Piping Systems at Consumer Locations
• CGA G-5.5, Hydrogen Vent Systems
• CGA G-5.6, Hydrogen Pipeline Systems
• CGA G-5.8, High Pressure Hydrogen Piping Systems at Consumer Locations.
• FM Global Property Loss Prevention Data Sheets 7-50: Compressed Gases in Cylinders
• FM Global Property Loss Prevention Data Sheets 7-91: Hydrogen
• IGC Doc 121/04/E, Hydrogen Transportation Pipelines System Design Features
• NASA
• NSS 1740.16 Safety Standard For Hydrogen And Hydrogen Systems Guidelines for
Hydrogen System Design, Materials Selection, Operations, Storage, and Transportation
• NFPA 52, Vehicular Fuel Systems Code
• NFPA 55, Standard for the Storage, Use, and Handling of Compressed Gases and
Cryogenic Fluids in Portable and Stationary Containers, Cylinders, and Tanks, 2005
Edition
• NFPA 68, Standard on Explosion Protection by Deflagration Venting
• NFPA 70, National Electrical Code
• NFPA 497, Recommended Practice for the Classification of Flammable Liquids, Gases,
or Vapors and of Hazardous (Classified) Locations for Electrical Installations in Chemical
Process Areas
• NFPA 13, Standard for the Installation of Sprinkler Systems
• NFPA 45, Standard on Fire Protection for Laboratories Using Chemicals
• NFPA 55, Standard for the Storage, Use, and Handling of Compressed Gases and
Cryogenic Fluids in Portable and Stationary Containers, Cylinders, and Tanks
• NFPA 68, 2007 Standard on Explosion Protection by Deflagration Venting
• NFPA 69, Standard on Explosion Prevention Systems
• NFPA 91, Standard for Exhaust Systems for Air Conveying of Vapors

xxviii TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Preface
Liquid Nitrogen Safety Precautions

• NFPA 255, Standard Method of Test of Surface Burning Characteristics of Building

Materials

OSHA 29CFR1910.103 1910.103 Hydrogen

Hazardous Substances Precautions

WARNING Before using hazardous substances (toxic, harmful, and so on), read the
hazard indications and information reported in the applicable Material Safety Data Sheet
(MSDS.) Use Personal protection according to the safety requirements.

Venting Toxic Gases

When analyzing toxic compounds be aware that during the normal operation of the GC some
of the sample might be vented outside the instrument through the inlet and detector exits;
therefore, make sure to vent the exhaust gases to a fume hood. Consult local Environmental
and Safety Regulations for instructions in exhausting fumes from your system.

Liquid Nitrogen Safety Precautions

Liquid nitrogen is a colorless, odorless, extremely cold liquid and gas under pressure. It can
cause rapid suffocation when concentrations are sufficient to reduce oxygen levels below
19.5%. A Self Contained Breathing Apparatus (SCBA) might be required. Contact with
liquid or cold vapors can cause severe frostbite. Cold vapors in the air will appear as a white
fog due to condensation of moisture. Oxygen concentrations must be monitored in the release
area. All cryogenic liquids produce large volumes of gas when they vaporize.

WARNING Before using Liquid Nitrogen, read the hazard indications and the
instructions reported in the Safety sheet supplied by the manufacturer, with reference to
the CAS number (Chemical Abstract Service) 7727-37-9.

Use personal protection:

• Protective gloves: Loose fitting thermal-insulated or leather gloves.
• Eye protection: Full face shield and safety glasses are recommended.
• Other protective equipment: Safety shoes when handling containers. Long sleeved
shirts and trousers without cuffs. Work clothing that sufficiently prevents skin contact
should be worn.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual xxix
Preface
Carbon Dioxide Safety Precautions

Carbon Dioxide Safety Precautions

Carbon dioxide is a colorless, cryogenic liquid. At low concentrations, is odorless. At higher
concentrations carbon dioxide will have a sharp, acidic odor. At concentrations between 2 and
10%, Carbon dioxide can cause nausea, dizziness, headache, mental confusion, increased
blood pressure, and increased respiratory rate. If the gas concentration reaches 10% or more,
suffocation and death can occur within minutes. Contact with the cold gas can cause freezing
of exposed tissue. Moisture in the air could lead to the formation of carbonic acid that can be
irritating to the eyes. All forms of carbon dioxide are noncombustible. Carbon dioxide is
heavier than air and should not be allowed to accumulate in low lying areas.

WARNING Before using carbon dioxide, read the indications of hazard and the
instructions reported in the Safety sheet supplied by the manufacturer with reference to
the CAS number (Chemical Abstract Service) 124-38-9.

Use personal protection:

• Protective gloves: Loose fitting thermal insulated or leather gloves.
• Eye protection: Full face shield and safety glasses are recommended.
• Other protective equipment: Safety shoes when handling containers. Long sleeved
shirts and trousers without cuffs. Work clothing that sufficiently prevents skin contact
should be worn.

xxx TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 1

Installation
This chapter provides instructions for installing and connecting the TRACE 1300/TRACE
1310 system.

Contents
• Positioning the TRACE 1300/TRACE 1310
• Installing the External Accessories
• Installing the Injector and Detector Modules
• Making the Gas Supply Plumbing Connections
• Connecting the Oven Cryogenic System
• Connecting the PTV/PTVBKF Cryogenic System
• Coupling to a Mass Spectrometer
• Installing the Autosampler
• Installing the Data System Software
• Making Power Connections
• Setting the LAN Communication
• Column Installation Requirements
• Installing the Column the First Time

IMPORTANT Before beginning the installation process, your laboratory must be in

compliance with the guidelines and the requirements described in the TRACE
1300/TRACE 1310 Preinstallation Requirements Guide.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 1

1 Installation

When you receive your TRACE 1300/TRACE 1310, an authorized Thermo Fisher Scientific
Field Service Engineer (FSE) will configure the system for you. However, if you need to
reconfigure your system, repeat the following steps:
1. Place the TRACE 1300/TRACE 1310 on the workbench.
a. See “Positioning the TRACE 1300/TRACE 1310” on page 3.
2. Place and connect the external accessories.
a. See “Installing the External Accessories” on page 3.
3. Place and connect the Injector and Detector modules.
a. See “Installing the Injector and Detector Modules” on page 4.
4. Plumb the gas supply and perform test for leaks.
a. See “Making the Gas Supply Plumbing Connections” on page 7.
b. For system test information, refer to the TRACE 1300 and TRACE 1310 User Guide
and to the User Guide of any accessories you have connected to your GC system.
5. Connect coolant to cryogenic system (if present).
a. See “Connecting the Oven Cryogenic System” on page 13.
6. Couple a TRACE 1300/TRACE 1310 version for MS to a mass spectrometer.
a. Perform this step to couple a GC MS version with a Thermo Scientific mass
spectrometer. See “Coupling to a Mass Spectrometer” on page 23.
7. Install the autosampler (optional) on the GC.
a. See “Installing the Autosampler” on page 32.
8. Install the Data System software.
a. See “Installing the Data System Software” on page 42
9. Connecting Power to the GC and the External Modules.
a. See “Making Power Connections” on page 43.
10. Set the LAN communication between TRACE 1300/TRACE 1310 and computer.
a. See “Setting the LAN Communication” on page 45.
11. Install the analytical column.
a. Install the column into GC as described in “Column Installation Requirements” on
page 50 and “Installing the Column the First Time” on page 57.
12. Perform column conditioning, leak test, and column evaluation.
a. Perform column conditioning, leak check, and carrier gas flow calibration (column
evaluation) following the instruction reported in “Installing the Column the First
Time” on page 57.

2 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Positioning the TRACE 1300/TRACE 1310

Positioning the TRACE 1300/TRACE 1310

Your laboratory must already be prepared according to the space requirements, and the gas
and power supplies must be accessible. Optional equipment should be placed near the
TRACE 1300/TRACE 1310 for easier connection.

 To position the instrument

WARNING The main unit of the GC, without injector/detector modules, weighs
approximately 35 kg (77 lbs) when unpacked. Pay attention when lifting the instrument
onto the work table.

1. Set the TRACE 1300/TRACE 1310 on the work table. At least TWO people should
perform this operation, each standing on one side of the instrument and putting their
hands near its supporting feet.

WARNING The oven vents at the back of the GC discharge hot air up to 450 °C (842 °F)
during cooling. Oven exhaust can cause severe burns. Avoid working behind the
instrument oven vents during cool-down cycles. There should be at least 30 cm (12 in.)
free space behind the instrument to allow the exhaust to dissipate. Do not expose gas tanks
or bottles, chemicals, regulators, electrical cables, or other temperature-sensitive items to
oven exhaust.

Note An optional oven exhaust kit can be installed to carry the hot air from the oven
vents out of the working area. See “Installing the Oven Exhaust Kit” on page 326.

2. Look for the yellow label located on the back of the GC under the AC Input Connector.
It indicates the power supply (120 Vac or 230 Vac) required by the GC, and must be in
compliance with your power source.

CAUTION To avoid contact with the hot air from the vents, gather the electrical cables
into the cables holder.

Installing the External Accessories

For additional information, refer to the TRACE 1300 and TRACE 1310 User Guide, and to
the User Guides that come with the accessories you have connected to your GC.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 3

1 Installation
Installing the Injector and Detector Modules

Installing the Injector and Detector Modules

This section provides the instructions for installing your front/back injector and detector
modules into the relevant housing.

The GC is shipped with dummy modules installed into the injector/detector housings.
See Figure 1.

Figure 1. Injector and Detector Housings and Dummy Modules

Detector Housing Injector Housing

Note Where a dummy module is installed, the gas connection is blocked by a plug.

The dummy modules must be removed and replaced by the injector and detector modules as
required by the configuration of your TRACE 1300/TRACE 1310.

CAUTION Each housing must always be occupied by both modules. If the GC is

configured with a single injector/detector module, a dummy module must remain
inserted instead of the missing injector/detector module.

The injector and detector modules are shipped with a plug. Before installing the column,
remove the plug.

To install the injector and detector modules, see the following procedures:
• Installing an Injector Module
• Installing a Detector Module

Installing an Injector Module

 To install an injector module

1. Remove the dummy module from the position where the injector module will be
installed.

4 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Installing the Injector and Detector Modules

a. Open the module flap cover.

b. Using a T20 Torxhead screwdriver, unscrew the two captive fixing screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
injector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connection by unscrewing its fixing screw
using a T20 Torxhead screwdriver.

Figure 2. Injector Gas Block Plug

Gas Block Plug on Injector Seat

O-ring

Gas Connection Without Gas Block Plug

WARNING Make sure the o-ring is placed into its seat on the gas connection.
See Figure 2. Do not install the module if the o-ring is missing.

2. Plug the injector module into the main frame.

a. Open the module flap cover.
b. Keeping the module flap cover open, place it in its seat. Be sure to insert the 25-pin
male connector on the bottom of the module into the 25-pin female connector on
the injector seat of the injector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws evenly and
carefully without overtightening.

CAUTION To maintain the correct alignment the screws must be tightened in turn, and
each screw must be tightened only a small amount before moving to the next screw.
Repeat until all are secure.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 5

1 Installation
Installing the Injector and Detector Modules

d. Close the module flap cover.

3. Continue the installation following the instructions reporting in the section “Making the
Gas Supply Plumbing Connections” on page 7.

Installing a Detector Module

CAUTION If you are installing a NPD detector module, the installation of the NPD
Thermionic Source Power Module is required. For the installation details see the section
“Adding a NPD Detector Module” on page 435.

CAUTION If you are installing a Generic Detector Interface see the section “Adding a
Generic Detector Interface” on page 472.

 To install a detector module

1. Remove the dummy module from the position where the detector module will be
installed.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the two captive fixing screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
injector/detector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connections by unscrewing its fixing screw
using a T20 Torxhead screwdriver.

Figure 3. Detector Gas Block Plug

Gas Block Plug on Detector Seat

O-rings

Gas Connections Without Gas Block Plug

6 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Making the Gas Supply Plumbing Connections

WARNING Make sure all the four o-rings are placed into their seats on the gas
connection. See Figure 3. Do not install the module if the o-rings are missing.

2. Plug the detector module into the main frame.

a. Open the module flap cover.
b. Keeping the module flap cover open, place the module in its seat.
Be sure to insert the 25-pin male connector on the bottom of the module into the
25-pin female connector on the detector seat of the detector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws evenly and
carefully without overtightening.

CAUTION To maintain the correct alignment the screws must be tightened in turn, and
each screw must be tightened only a small amount before moving to the next screw.
Repeat until all are secure.

d. Close the module flap cover.

3. Continue the installation following the instructions in the section “Making the Gas
Supply Plumbing Connections” on page 7.

Making the Gas Supply Plumbing Connections

Make plumbing connections between the gas supply lines and the GC gas inlets on the
instrument back using the proper fittings

CAUTION DO NOT loosen or remove caps from the TRACE 1300/TRACE 1310 until
you have purged gas lines, and you are ready to connect them. Loosening or removing
caps early will contaminate instruments and filters.

WARNING Before using gases, carefully read the hazard indications and information
reported in the Safety Sheet supplied by the manufacturer referring to the CAS (Chemical
Abstract Service) number. It is the user’s responsibility to see that all local safety
regulations for the use of gases are obeyed.

All Thermo Fisher Scientific gas chromatographs use an inert gas as the carrier gas. If you
wish to use hydrogen as a carrier gas, you must install a hydrogen sensor. Contact a
Thermo Fisher Scientific sales representative if you plan to use hydrogen as the carrier gas
in your new TRACE 1300/TRACE 1310. If you don’t have the hydrogen sensor, you
must use an inert carrier gas.

Thermo Fisher Scientific FSEs are not authorized to install or repair any instrument using
hydrogen as a carrier gas unless the instrument is equipped with the appropriate sensor.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 7

1 Installation
Making the Gas Supply Plumbing Connections

 To plumb the gas supply

1. By now you must have done the following:

a. Completely built your gas supply lines including any traps, tees, and extra tubing to
allow about 40 cm (16 in.) of slack in the line.
b. Purged the gas line after every tube cut to remove any debris or contaminants.
c. Ensured the gas supply is turned off.
2. Connect the gas lines.
a. TRACE 1300/TRACE 1310 is provided with six gas inlet ports for the connection of
carrier and detector gases. See Figure 4.

Figure 4. Gases Inlet Connections

Carrier Front
Gas Inlet Ports

Carrier Back
Detector Gases

Hydrogen
Air
Make-up Front
Make-up Back

Note Use the 1/8-in. Swagelok fittings provided on the gas inlet ports (see Figure 5)
to connect the gas lines.

Figure 5. Fittings for Gas Inlets Connection

Nut Ferrules Gas Inlet Fitting O-Ring

8 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Making the Gas Supply Plumbing Connections

CAUTION Inside each 1/8-in. inlet manifold is a fritted filter. To keep the gas line from
touching, and possibly damaging the filter, extend the tubing only 5-mm past the front
ferrule. This ensures the tubing does not touch the filter.

b. If present, remove the cap from the gas inlet port to connect. See Figure 6.

Figure 6. Cap Removal

Gas Inlet Port

Cap

c. If not already installed, screw the gas inlet fitting provided into the gas inlet port
interposing the o-ring. See Figure 7.

Figure 7. Gas Inlet Fitting Installation

O-Ring

Gas Inlet Fitting

d. Connect the gas line to the front/back carrier gas inlet. Use a 7/16-in. wrench for
tightening the fittings. See Figure 8.

Figure 8. Carrier Gas Line Connection

Back Carrier Gas Inlet

Front Carrier Gas Inlet

e. Connect the gas line to the front/back detector gas inlet. Use a 7/16-in. wrench for
tightening the fittings. See Figure 9, Figure 10, Figure 11, and Figure 12 to properly
connect each detector.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 9

1 Installation
Making the Gas Supply Plumbing Connections

Figure 9. FID/NPD Detector Gas Lines Connection

Front Detector Gas Inlets

Back Detector Gas Inlets

Figure 10. ECD Detector Gas Lines Connection

Front Detector Gas Inlet

Back Detector Gas Inlet

Figure 11. TCD Detector Gas Lines Connection

Back Carrier Gas Inlet

Front Carrier Gas Inlet

10 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Making the Gas Supply Plumbing Connections

Figure 12. FPD Detector Gas Lines Connection

Back Carrier Gas Inlet

f. Be sure to complete all inlet and detector connections before turning on the gas
supply.
3. Supply the gas lines.

IMPORTANT The maximum nominal inlet pressure for all the inputs is 1000 kPa
(145 psig), as indicated on the label under the gas inlets ports on the back of the GC.
The working inlet pressure range is from 400 kPa (58 psig) to 1000 kPa (145 psig).

Note If auxiliary carrier gases are required, the installation of the Auxiliary Gas module is
required. For the installation details, see the section “Adding an Auxiliary Gas System” on
page 521.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 11

1 Installation
Making the Gas Supply Plumbing Connections

Testing for Leaks

Once you have connected the gas supplies to the GC, you need to test the gas supply lines for
leaks.

CAUTION Before performing a leak test make sure that the GC is powered off and the
power cable is unplugged from the AC Input connector (Mains socket) and from the wall
outlet.

 To perform a leak test

1. Be sure that GC is powered off.

2. Open and set the gas supply.
a. Open the gas supply.
b. Set the carrier gas pressure to approximately 50 kPa (7 psi) higher than the maximum
pressure of the GC regulator.
c. Set the detector gas pressures to approximately 1000 kPa (145 psig) if available,
otherwise set the maximum pressure allowed, for example 500 kPa (72.5 psi).
3. Check for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check each fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.
c. Repeat this process until all connections are leak free.

12 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Connecting the Oven Cryogenic System

Connecting the Oven Cryogenic System

If the oven of your TRACE 1300/TRACE 1310 is equipped with a cryogenic cooling option,
you need a supply of coolant, such as carbon dioxide (CO2) or liquid nitrogen (N2).

Oven Cryo System with Carbon Dioxide Connection

This Oven cryo system consists of a tube for carbon dioxide inserted into the oven and fitted
with a 1/8-in. solenoid valve for carbon dioxide mounted into the back of the GC.
Carbon dioxide must be supplied in a high-pressure cylinder with a dip tube. It is your
responsibility to ensure the delivery connection from the carbon dioxide cryogenic supply is
adaptable to 1/8-in. tubing. Figure 13 shows the proper carbon dioxide container
configuration.

Figure 13. Carbon Dioxide Container

CORRECT WRONG

Dip tube

CAUTION High pressures and extremely low temperatures make pressurized carbon
dioxide a hazardous material. High concentrations of carbon dioxide are dangerous.
To avoid injury, always follow the safety precautions and delivery system design
recommended by your gas supplier.

 To connect the cryogenic system to the carbon dioxide supply

1. Connect the proper end of the cryo supply tube to the 1/8-in. NPT connection of the
solenoid valve using the proper nut and ferrule. Use a 7/16-in. wrench to tighten the
fittings. See Figure 14.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 13

1 Installation
Connecting the Oven Cryogenic System

Figure 14. Oven Cryo Supply Tube for Carbon Dioxide Connection

Nut Ferrule 1/8-in. NPT

Fittings for the connection to the carbon dioxide tank

2. Connect the other end of the cryo supply tube to the coolant container using the
appropriate nut and ferrule.

14 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Connecting the Oven Cryogenic System

3. Connect the cryo valve to the Aux Temperature/Cryo Module.

IMPORTANT The Auxiliary Temperature/Cryo must be powered at the same line voltage
of the main GC system.

a. Connect the cryo solenoid valve to the 2-pin connector marked Cryo Valves - Oven
using the cable provided. See Figure 15.

Figure 15. Cryo Valves: Oven

Oven Cryo Valve

b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights up after the GC power on.

Note For further details regarding the installation of the Aux Temperature/Cryo module,
see the section “Adding an Aux Temperature/Cryo Module” on page 443.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 15

1 Installation
Connecting the Oven Cryogenic System

Oven Cryo System with Liquid Nitrogen Connection

This Oven cryo system consists of a tube for liquid nitrogen inserted into the oven and fitted
with a 1/8-in. solenoid valve for liquid nitrogen mounted into the back of the GC.

The tube for liquid nitrogen is covered with an insulating material tube.

Liquid Nitrogen must be supplied at a pressure of 1.5 bar (150 kPa; 21.75 psig). Plumbing to
the GC should be 1/4-in. copper or stainless steel tubing with insulation. It is your
responsibility to ensure the delivery connection from the liquid nitrogen cryogenic supply is
adaptable to 1/4-in. tubing. The liquid nitrogen cryogenic valve on the GC is a 1/4-in.
Swagelok fitting.
Figure 24 shows the proper configuration for a liquid nitrogen tank.

Figure 16. Liquid Nitrogen Tank Configuration

WARNING High pressures and extremely low temperatures make liquid nitrogen a
hazardous material. High concentrations of liquid nitrogen in the air can be an
asphyxiation hazard. To avoid injury, always follow the safety precautions and delivery
system design recommended by your gas supplier.

 To connect the cryogenic system to the liquid nitrogen supply

1. Connect the proper end of the cryo supply tube to the 1/8-in. NPT connection of the
solenoid valve using the proper nut and ferrule. Use a 7/16-in. wrench to tighten the
fittings. See Figure 17.

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 1 Installation
Connecting the Oven Cryogenic System

Figure 17. Oven Cryo Supply Tube for Liquid Nitrogen Connection

Nut Ferrule 1/8-in. NPT

Fittings for the connection to the Liquid Nitrogen Tank

2. Connect the other end of the cryo supply tube to the coolant container using the
appropriate nuts and ferrules.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 17

1 Installation
Connecting the PTV/PTVBKF Cryogenic System

3. Connect the cryo valve to the Aux Temperature/Cryo Module.

IMPORTANT The Auxiliary Temperature/Cryo must be powered at the same line voltage
of the main GC system.

a. Connect the cryo solenoid valve to the 2-pin connector marked Cryo Valves - Oven
using the cable provided. See Figure 18.

Figure 18. Cryo Valves: Oven

Oven Cryo Valve

b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights up after the GC power on.

Note For further details regarding the installation of the Aux Temperature/Cryo module,
see the section “Adding an Aux Temperature/Cryo Module” on page 443.

Connecting the PTV/PTVBKF Cryogenic System

If the PTV or PTVBKF injector of your TRACE 1300/TRACE 1310 is equipped with a
cryogenic cooling option, you need a supply of coolant, carbon dioxide (CO2) or liquid
nitrogen (LN2).

PTV/PTVBKF Cryo System with Carbon Dioxide Connection

This cryo system consists of a tube for Carbon Dioxide inserted into the PTV/PTVBKF
module, and fitted with a 1/8-in. solenoid valve for carbon dioxide mounted on a bracket
fixed on the back of the GC. See Figure 19.

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 1 Installation
Connecting the PTV/PTVBKF Cryogenic System

Figure 19. Front and Back PTV Cryo System with CO2

Carbon dioxide must be supplied by a high-pressure cylinder with a dip tube.

It is your responsibility to ensure the delivery connection from the carbon dioxide cryogenic
supply is adaptable to 1/8-in. tubing. Figure 20 shows the proper carbon dioxide container
configuration.

Figure 20. Carbon Dioxide Container

CORRECT WRONG

Dip tube

CAUTION High pressures and extremely low temperatures make pressurized CO2 a
hazardous material. High concentrations of CO2 are dangerous.
To avoid injury, always follow the safety precautions and delivery system design
recommended by your gas supplier.

 To connect the cryogenic system to the carbon dioxide supply

1. Connect the proper end of the cryo supply tube to the 1/8-in. NPT connection of the
solenoid valve using the proper nut and ferrule. Use a 7/16-in. wrench to tighten the
fittings. See Figure 21.

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1 Installation
Connecting the PTV/PTVBKF Cryogenic System

Figure 21. PTV/PTVBKF Cryo Supply Tube for Carbon Dioxide Connection

Solenoid Valve
Solenoid Valve
Bracket

1/8-in. NPT
Ferrule
Nut

2. Connect the other end of the cryo supply tube to the coolant container using the
appropriate nuts and ferrules.
3. Connect the cryo valve to the Aux Temperature/Cryo Module.

IMPORTANT The Aux Temperature/Cryo module must be powered at the same line
voltage of the main GC system.

a. Connect the 2-pin connector marked Cryo Valves - Front Inlet or Cryo Valves-Back
Inlet to the cryo solenoid valve using the cable provided. See Figure 22.

Figure 22. Cryo Valves: Front/Back Inlet

Back Inlet Cryo Valve

Front Inlet Cryo Valve

b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights up after the GC power on.

Note For further details regarding the installation of the Aux Temperature/Cryo module,
see the section “Adding an Aux Temperature/Cryo Module” on page 443.

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 1 Installation
Connecting the PTV/PTVBKF Cryogenic System

PTV/PTVBKF Cryo System with Liquid Nitrogen Connection

This cryo system consists of a tube for liquid nitrogen inserted into the PTV/PTVBKF
module and fitted with a 1/8-in. solenoid valve for liquid nitrogen mounted on a bracket
fixed on the back of the GC. The tube for liquid nitrogen is covered with an insulating
material tube. See Figure 23.

Figure 23. Front and Back PTV Cryo System with Liquid Nitrogen

Liquid nitrogen must be supplied at a pressure below 400 kPa (60 psi). Plumbing to the GC
should be 1/4-in. copper or stainless steel tubing with insulation.
It is your responsibility to ensure the delivery connection from the Liquid Nitrogen cryogenic
supply is adaptable to 1/4-in. tubing. The liquid nitrogen cryogenic valve on the GC is a
1/4-in. Swagelok fitting.

Figure 24 shows the proper configuration for a liquid nitrogen tank.

Figure 24. Liquid Nitrogen Tank Configuration

WARNING High pressures and extremely low temperatures make Liquid Nitrogen a
hazardous material. High concentrations of liquid nitrogen in the air can be an
asphyxiation hazard. To avoid injury, always follow the safety precautions and delivery
system design recommended by your gas supplier.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 21

1 Installation
Connecting the PTV/PTVBKF Cryogenic System

 To connect the cryogenic system to the liquid nitrogen supply

1. Connect the proper end of the cryo supply tube to the 1/8-in. NPT connection of the
solenoid valve using the proper nut and ferrule. Use a 7/16-in. wrench to tighten the
fittings. See Figure 21.

Figure 25. PTV/PTVBKF Cryo Supply Tube for LN2 Connection

Solenoid Valve
Solenoid Valve
Bracket

1/8-in. NPT

Ferrule

Nut

2. Connect the other end of the cryo supply tube to the coolant container using the
appropriate nuts and ferrules.
3. Connect the cryo valve to the Aux Temperature/Cryo Module.

IMPORTANT The Auxiliary Temperature/Cryo must be powered at the same line voltage
of the main GC system.

a. Connect the 2-pin connector marked Cryo Valves - Front Inlet or Cryo Valves-Back
Inlet to the cryo solenoid valve using the cable provided. See Figure 26.
Figure 26. Cryo Valves: Front/Back Inlet

Back Inlet Cryo Valve

Front Inlet Cryo Valve
b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.

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 1 Installation
Coupling to a Mass Spectrometer

c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights up after the GC power on.
]

Note For further details regarding the installation of the Aux Temperature/Cryo module,
see the section “Adding an Aux Temperature/Cryo Module” on page 443.

Coupling to a Mass Spectrometer

This section provides instruction for opening the duct to introduce the transfer line inner
tube into the oven of your TRACE 1300/TRACE 1310 version for MS.
According to the mass spectrometer unit to couple, see the following sections:
• “Making the Duct to Couple with an ISQ Series, TSQ 8000 Series, DSQ II, or ITQ
Mass Spectrometer” on page 23.
• “Making the Duct to Couple with a TSQ Quantum Mass Spectrometer” on page 26.
• “Making the Duct to Couple with a DFS, IRMS, or ICP-MS Mass Spectrometer” on
page 29.

Making the Duct to Couple with an ISQ Series, TSQ 8000 Series, DSQ II, or ITQ Mass
Spectrometer
 To make the duct for the transfer line

1. Remove the left side panel.

a. Open the front door of the GC.
b. Use a T20 Torxhead screwdriver to loosen the left side panel screw from the interior
front panel. See Figure 27. Save the screw because it will be reused later.

Figure 27. Left Side Panel Fixing Screw

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1 Installation
Coupling to a Mass Spectrometer

c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel by pulling it outward. Be aware that the ground wire is attached to
the panel.
2. Prepare the duct for the ISQ Series, TSQ 8000 Series, DSQ II, or ITQ transfer line inner
tube.
a. Remove the partial cut plate on the left panel for that corresponds to your MS.
See Figure 28.
Figure 28. Left Panel for MS Partial Cut Plate

Duct for ISQ Series , TSQ 8000

Series, and DSQ II Partial Cut
Plate

Duct for ITQ Partial Cut Plate

b. On the exterior wall of the oven box, remove the pre-shaped plug of insulating
material from the duct provided. See Figure 29.

Figure 29. Pre-shaped Plug Removal

Duct for DSQ II Pre-shaped Plug

Duct for ISQ Series /TSQ 8000
Series Pre-shaped Plug

Duct for ITQ Pre-shaped Plug

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 1 Installation
Coupling to a Mass Spectrometer

IMPORTANT Save the pre-shaped plug of insulating material in a safe place because it
could be reused.

c. On the interior of the oven box remove the partial cut plate from the corresponding
duct. See Figure 30.

Figure 30. Ducts for the Coupling with ISQ Series, TSQ 8000 Series, DSQ II and ITQ Mass
Spectrometer

ITQ
DSQ II

ISQ Series and TSQ 8000

Series

d. Place the left panel for your MS and attach the screw holding it in place.
3. Introduce the transfer line inner tube into the oven through the duct provided.
4. Attach the transfer line to the GC column using the proper nut and ferrule.

CAUTION - INSTRUMENT DAMAGE: Make sure that the GC column has been
conditioned before connecting it to the transfer line. The material released from the
column, (column bleed), during conditioning may contaminate the detector.

5. Close the front door of the GC.

6. For DSQ II and ITQ, connect the transfer line heater to the Aux Temperature/Cryo
Module. See Figure 31.

CAUTION An extension cable transfer line is needed when connecting an ITQ mass
spectrometer to the Aux Temperature/Cryo module.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 25

1 Installation
Coupling to a Mass Spectrometer

Figure 31. Transfer Line MS Heaters

Heater Connection for

Transfer Line 2
Heater Connection for
Transfer Line 1

7. To tune and set the ISQ Series, TSQ 8000 Series, DSQ II, and ITQ working conditions,
refer to the relevant User Guide and Hardware Manual.

Making the Duct to Couple with a TSQ Quantum Mass Spectrometer

 To make the duct for the transfer line

1. Remove the right side panel.

a. Open the front door of the GC.
b. Use a T20 Torxhead screwdriver to loosen the right side panel screw from the interior
front panel. See Figure 32. Save the screw because it will be reused later.

Figure 32. Right Side Panel Fixing Screw

c. Slide the panel towards the back of the instrument up to the stop.

26 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Coupling to a Mass Spectrometer

d. Remove the panel by pulling it outward. Be aware that the ground wire is attached to
the panel.
2. Prepare the duct for the TSQ Quantum transfer line inner tube.
a. Use a T20 Torxhead screwdriver to loosen the duct plate screws from the GC right
panel of the MS. See Figure 33.

Figure 33. Right Panel for MS

Duct Plate

b. On the exterior wall of the oven box, remove the pre-shaped plug of insulating
material from the duct provided. See Figure 34.

Figure 34. Pre-shaped Plug Removal

Duct for TSQ Quantum Pre-shaped Plug

IMPORTANT Save the pre-shaped plug of insulating material in a safe place because it
could be reused.

c. On the interior of the oven box remove the partial cut plate from the corresponding
duct. See Figure 35.

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1 Installation
Coupling to a Mass Spectrometer

Figure 35. Duct for the Coupling with a TSQ Quantum Mass Spectrometer

TSQ Quantum

d. Place the GC right panel for your MS and attach the screw holding it in place.
3. Introduce the transfer line inner tube into the oven through the duct provided.
4. Attach the transfer line to the GC column using the proper nut and ferrule.

CAUTION - INSTRUMENT DAMAGE: Make sure that the GC column has been
conditioned before connecting it to the transfer line. The material released from the
column, (column bleed), during conditioning may contaminate the detector.

5. Close the front door of the GC.

6. Connect the transfer line heater to the Aux Temperature/Cryo Module. See Figure 36.
Figure 36. Transfer Line MS Heaters

Heater Connection for

Transfer Line 2
Heater Connection for
Transfer Line 1

7. To tune and set the TSQ Quantum working conditions, refer to the relevant User Guide
and Hardware Manual.

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 1 Installation
Coupling to a Mass Spectrometer

Making the Duct to Couple with a DFS, IRMS, or ICP-MS Mass Spectrometer
 To make the duct for the transfer line

1. Remove the left/right side panel according to the high resolution mass spectrometer to
couple.
a. Open the front door of the GC.
b. Use a T20 Torxhead screwdriver to loosen the left/right side panel screw from the
interior front panel. Save the screw because it will be reused later.
c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel by pulling it outward. Be aware that the ground wire is attached to
the panel.
2. Prepare the proper duct for the transfer line inner tube.
a. Use a T20 Torxhead screwdriver to loosen the duct plate screws from the left/right
panel. See Figure 37.

Figure 37. Left/Right Side Panel

Left Side Panel Right Side Panel

Duct Plate

b. According to the configuration of your high resolution mass spectrometer, on the

left/right exterior wall of the oven box, remove the partial cut plate of the duct of
interest, and then the pre-shaped plug of insulating material from the duct provided.
See Figure 38.

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1 Installation
Coupling to a Mass Spectrometer

Figure 38. Left/Right Exterior Oven Wall Box View

Left Oven Wall Right Oven Wall

IMPORTANT Save the pre-shaped plug of insulating material in a safe place because it
could be reused.

c. On the interior of the oven box remove the partial cut plate from the corresponding
duct. See Figure 39.

Figure 39. Left/Right Interior Oven Wall Box View

Left Oven Wall

Right Oven Wall

d. Place the left/right panel for MS and attach the screw holding it in place.
3. Introduce the transfer line inner tube into the oven through the duct provided.
4. Attach the transfer line to the GC column using the proper nut and ferrule.

CAUTION - INSTRUMENT DAMAGE: Make sure that the GC column has been
conditioned before connecting it to the transfer line. The material released from the
column (column bleed) during conditioning may contaminate the detector.

5. Close the front door of the GC.

6. Connect the transfer line heater if required.
a. Connect the transfer line heater to the Aux Temperature/Cryo Module. See
Figure 40.

30 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Coupling to a Mass Spectrometer

Figure 40. Transfer Line MS Heaters

Heater Connection for

Transfer Line 2
Heater Connection for
Transfer Line 1

7. Tune and set the DFS, IRMS, and ICP-MS working conditions of the high resolution
mass spectrometer, referring to the relevant manuals.

Setting Handshake Parameters

When your GC is connected to a mass spectrometer, set the handshaking parameters as shown
in Table 1.
Table 1. GC Handshaking Parameters When A Mass Spectrometer is Connected
Parameter Set To:
Remote Start In High to Low
Inhibit Ready In When High
End of Run Out High to Low
Start of Run Out High to Low
GC Ready Out When Low
Prep Run Out When Low

 To configure handshaking parameters through the touch screen

1. In the main menu select the Configuration icon, the Configuration menu appears.
2. In the Configuration menu, select the Handshake icon to open the relevant submenu.
3. Set the Handshaking parameters as required, then return to main menu.

 To configure handshaking parameters through the Chromatography Data System

1. Launch the Data System. In the relevant Configuration page specify the handshaking
parameters.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 31

1 Installation
Installing the Autosampler

Installing the Autosampler

This section provides instruction for installing and connecting a TriPlus RSH, a TriPlus 100
Liquid Sampler, a TriPlus, an AI/AS 1310 - AI/AS 3000 II, or two AI/AS 1310 - AI/AS 3000
II in Gemini configuration.

Related Topics
• “Mounting an Autosampler on the GC” on page 32
• “Connecting the Autosampler” on page 34

Mounting an Autosampler on the GC

To mount the autosampler use the appropriate support and fix it to the installation holes
provided on the top of the GC. See Figure 41 and Figure 42.

For further details please refer to the manuals of your sampling system.

Figure 41. Installation Holes and Sampler Supports for TriPlus RSH and TriPlus 100 Liquid Sampler

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 1 Installation
Installing the Autosampler

Figure 42. Installation Holes and Sampler Supports for AI/AS 1310 - AI/AS 3000 II Autosampler

Note: The support pin is required when an

AS 1310 must be installed.

Support Pin

Support and Installation Holes for the

AI 1310/AS 1310on the Back Injector
Module.

Support Pin

Support and Installation Holes for the

AI 1310/AS 1310 on the Front Injector
Module.

Support Pin

Support and Installation Holes for Two

AI 1310/AS 1310 in Gemini Configuration.

Support and Installation Holes for One or

Two AI 1310/AS 1310 when a TSQ Quantum
Mass Spectrometer is coupled with the GC.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 33

1 Installation
Installing the Autosampler

Connecting the Autosampler

There are two possible cabling patterns for connecting the TRACE 1300/TRACE 1310 to a
single autosampler:
• The GC and a TriPlus, AI/AS 1310, or AI/AS 3000 II autosampler are controlled via a
single serial cable connected. In this case, a RS-232 connection is also needed between the
GC and the autosampler.
• In the Chromatography Data System (CDS) Serial Port configuration window, this
option is termed Through GC. Through GC is the default connection. See Figure 43.

Figure 43. Through GC Connection

Serial Cable Handshake Cable

Autosampler

• The GC is connected to the Chromatography Data System (CDS) via LAN cable.
The autosampler is connected to the GC via RS-232 serial line and handshake cables.
See Figure 44.

Figure 44. Separated Connections

LAN Cable Serial Cable Handshake Cable

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 1 Installation
Installing the Autosampler

When two AI/AS 1310 or AI/AS 3000 II autosamplers are in use in Gemini
configuration, perform the hardware setup (HWSetup 1 or HWSetup 2) according to
the Thermo Scientific Chromatography Data System in use, and to the High
Throughput, Single, or Confirmation operating mode. See Figure 45, Figure 46, and
Table 2.

Figure 45. Connections of Two AI/AS Autosamplers - HWSetup 1

Hardware Setup 1

Gemini Y-shape Cable

Autosampler
Serial Cable
Twin Cable

CDS

Serial Cable

Figure 46. Connections of Two AI/AS Autosamplers - HWSetup 2

Hardware Setup 2

Serial Y-shape Autosampler Gemini Y-shape Cable

Cable
Twin Cable

CDS

Table 2. Hardware Setup Comparison Table (Sheet 1 of 2)

Chromatography Data System High Throughput Single Confirmation
(CDS) Mode Mode1 Mode
Chromeleon 7.2 HWSetup 1 HWSetup 1 HWSetup 2
See Figure 49 See Figure 49 See Figure 49
Xcalibur NA2 HWSetup 21 HWSetup 2
See Figure 50 See Figure 50

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 35

1 Installation
Installing the Autosampler

Table 2. Hardware Setup Comparison Table (Sheet 2 of 2)

Chromatography Data System High Throughput Single Confirmation
(CDS) Mode Mode1 Mode
TraceFinder 3.2 NA2 HWSetup 21 HWSetup 2
See Figure 50 See Figure 50
Chrom-Card 2.12 Alternative HWSetup 21 HWSetup 2
See Figure 50 See Figure 50 See Figure 50
ChromQuest 5; SP2015 R2 HWSetup 1 HWSetup 1 HWSetup 1
See Figure 49 See Figure 49 See Figure 49
EzChrom 3.22; SP2015 R2 HWSetup 1 HWSetup 1 HWSetup 1
See Figure 49 See Figure 49 See Figure 49
1 The unplugging of the power cord from the unused AI 1310/AS 1310 autosampler could be required
2
Not Applicable

• The GC and the TriPlus RSH or TriPlus 100 Liquid Sampler autosampler are connected
to the Chromatography Data System (CDS) via LAN cable.
The autosampler is connected to the GC through the Y-shape handshake cable. See
Figure 47 and Figure 48.

Figure 47. Connection of GC and TriPlus RSH/ TriPlus 100 Liquid Sampler via LAN

GC

Ethernet
Cable
CDS Y-shape Handshake Cable GC 1 X
Ethernet
Cable

TriPlus RSH
TriPlus 100 LS

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 1 Installation
Installing the Autosampler

Figure 48. Connection of TriPlus RSH/ TriPlus 100 Liquid Sampler to two GC via LAN

First GC
GC 1
Ethernet Cable
Y-shape Handshake Cable

Second GC
Ethernet GC 2
Ethernet Cable
Hub/Switch
CDS

Ethernet Cable

TriPlus RSH
TriPlus 100 LS

Connect the Autosampler to the GC System

To connect an autosampler to the GC system see the following procedures:
• “To connect a TriPlus RSH/TriPlus 100 Liquid Sampler autosampler” on page 38
• “To connect a second GC coupled with the same TriPlus RSH/TriPlus 100 Liquid
Sampler autosampler” on page 38
• “To connect a a TriPlus autosampler” on page 38
• “To connect a second GC is also coupled with the same TriPlus autosampler” on page 38
• “To connect an AI/AS 1310 - AI/AS 3000 II autosampler” on page 39
• “To perform the HWSetup 1 of two AI/AS 1310 - AI/AS 3000 II in Gemini
Configuration” on page 39
• “To perform the HWSetup of two AI/AS 1310 - AI/AS 3000 II in Gemini
Configuration” on page 40

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1 Installation
Installing the Autosampler

 To connect a TriPlus RSH/TriPlus 100 Liquid Sampler autosampler

1. Use the Ethernet cable to connect the LAN connector on the back of the TriPlus
RSH/TriPlus 100 Liquid Sampler Control Board to the computer, and verify that the
LAN control LED lights up when connected to a powered-on computer.
2. Using the Y-shape handshake cable provided, connect:
a. the main connector to the connection marked INTERFACE on the back of the
control interface of the TriPlus RSH/TriPlus 100 Liquid Sampler.
b. the first branch of the Y-shape handshake cable, labelled GC 1 to the marked
AUTOSAMPLER HANDSHAKE on the back panel of the GC.
3. Set the Primary/Secondary switch on PRIMARY position.
4. Establish communication between the TriPlus RSH/TriPlus 100 Liquid Sampler and the
GC following the instructions detailed in the TriPlus RSH Hardware Manual or TriPlus
100 Liquid Sampler Hardware Manual.

 To connect a second GC coupled with the same TriPlus RSH/TriPlus 100 Liquid
Sampler autosampler
1. Connect the second branch of the Y-shape handshake cable, labelled GC 2, to the
connector marked AUTOSAMPLER HANDSHAKE on the back panel of the second
GC.
2. Set the Primary/Secondary switch on SECONDARY position.
3. Establish communication between the TriPlus RSH/TriPlus 100 Liquid Sampler and the
GC following the instructions detailed in the TriPlus RSH Hardware Manual or TriPlus
100 Liquid Sampler Hardware Manual.

 To connect a a TriPlus autosampler

1. Using the cable provided, connect the 6-pin female connector marked HANDSHAKE
MAIN located on the back portion of the crossrail X and the connector marked
AUTOSAMPLER HANDSHAKE on the GC back panel.
2. Using the cable provided, connect the 9-pin female connector marked RS232-1 located
on the back portion of the crossrail X to 9-pin connector marked AUTOSAMPLER 1 on
the GC back panel.
3. Connect the power cable to the AC Input connector (Mains socket) on the autosampler
and to the wall outlet.

 To connect a second GC is also coupled with the same TriPlus autosampler

1. Using the cable provided, connect the 6-pin female connector marked HANDSHAKE
VIRTUAL located on the back portion of the crossrail X to the connector marked
AUTOSAMPLER HANDSHAKE on the back panel of the second GC.

38 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Installing the Autosampler

2. Using the cable provided, connect the 9-pin female connector marked RS232-2 located
on the back portion of the crossrail X to the 9-pin connector marked AUTOSAMPLER 2
on the GC back panel.
3. Connect the power cable to the AC Input connector (Mains socket) on the autosampler
and to the wall outlet.

 To connect an AI/AS 1310 - AI/AS 3000 II autosampler

1. Using the cable provided, connect the 6-pin female connector marked GC located on the
sampling unit back side to the connector marked AUTOSAMPLER HANDSHAKE on
the GC back panel.
2. Using the cable provided, connect the 9-pin female connector marked RS232 located on
the sampling unit back side to the 9-pin connector marked AUTOSAMPLER 1 on the
GC back panel.
3. Connect the power cable to the AC Input connector (Mains socket) on the autosampler
and to the wall outlet.

 To perform the HWSetup 1 of two AI/AS 1310 - AI/AS 3000 II in Gemini Configuration

Figure 49 shows the hardware setup 1 of two AI/AS 1310 or AI/AS 3000 II in Gemini
configuration.

Figure 49. Gemini Configuration - Hardware Setup 1

AI/AS 1310 - AI/AS 3000 II) (aAI/AS 1310 - AI/AS 3000 II)

TWIN TWIN
SYNC SYNC
TR AY RS 232 GC TR AY RS 232 GC
24 Vdc 24 Vdc

- + - +

USB RS232 USB RS232 SAMPLERSIGNAL

Adapter Adapter
COM 1 COM 2

USB USB

PC GC
1. Using the cable provided (PN 23043672), connect the 4-pin male connector marked
TWIN SYNC, on the back of the sampling unit of the sampler “a”, to the 4-pin male
connector marked TWIN SYNC on the back of the sampling unit of the sampler “b”.
2. Using the Gemini Y shape cable provided (PN 23043623), connect:

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 39

1 Installation
Installing the Autosampler

a. the 6-pin main connector labelled GC to the connector marked AUTOSAMPLER

HANDSHAKE on the back of the GC.
b. the first branch of the cable, labelled AI/AS 1, to the 6-pin female connector marked
GC on the back of the sampling unit of the sampler “a”.
c. the second branch of the cable, labelled AI/AS 2, to the 6-pin female connector
marked GC on the back of the sampling unit of the sampler “b”.
3. Using the RS232 cable (PN 23043453) provided in the standard outfit of each AI/AS
1310, connect:
a. the 9-pin connector marked RS232 of the first sampler to a PC serial port (e.g. COM
1), or to an USB port, interposing an USB-RS232 adapter not provided.
b. the 9-pin connector marked RS232 of the second sampler to a second PC serial port
(e.g. COM 2), or to an second USB port, interposing a second USB-RS232 adapter
not provided.
4. Connect the power cable to the AC Input connector (Mains socket) on the autosampler
and to the wall outlet.

 To perform the HWSetup of two AI/AS 1310 - AI/AS 3000 II in Gemini Configuration

Figure 50 shows the hardware setup 2 of two AI/AS 1310 or AI/AS 3000 II in Gemini
configuration.

Figure 50. Gemini Configuration - Alternative Hardware Connection (AHC)

AI/AS 1310 - AI/AS 3000 II) (aAI/AS 1310 - AI/AS 3000 II)

TWIN TWIN
SYNC SYNC
TR AY RS 232 GC TR AY RS 232 GC
24 Vdc 24 Vdc

- + - +

USB RS232dapter
U A

SAMPLERSIGNAL
USB Port
COM 1

PC GC
1. Using the cable provided (PN 23043672), connect the 4-pin male connector marked
TWIN SYNC, on the back of the sampling unit of the sampler “a”, to the 4-pin male
connector marked TWIN SYNC on the back of the sampling unit of the sampler “b”.

40 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Installing the Autosampler

2. Using the Gemini Y shape cable provided (PN 23043623), connect:

a. the 6-pin main connector labelled GC to the connector marked AUTOSAMPLER
HANDSHAKE on the back of the GC.
b. the first branch of the cable, labelled AI/AS 1, to the 6-pin female connector marked
GC on the back of the sampling unit of the sampler “a”.
c. the second branch of the cable, labelled AI/AS 2, to the 6-pin female connector
marked GC on the back of the sampling unit of the sampler “b”.
3. Using the Serial Y shape cable provided (PN 23043675), connect:
a. the 9-pin main connector to a PC serial port (e.g. COM 1) or to an USB port,
interposing an USB-RS232 adapter not provided.
b. the first branch of the cable, labelled AI/AS Primary, to the 9-pin female connector
marked RS232 on the back of the sampling unit of the sampler “a”.
c. the second branch of the cable, labelled AI/AS Secondary, to the 6-pin female
connector marked RS232 on the back of the sampling unit of the sampler “b”.
4. Connect the power cable to the AC Input connector (Mains socket) on the autosampler
and to the wall outlet.

Setting Autosampler Handshake Parameters

When your GC is connected to a AI/AS 1310, AI/AS 3000, TriPlus, TriPlus RSH, or TriPlus
100 Liquid Sampler set the handshaking parameters as shown in Table 3.

Table 3. Handshaking Parameters When An Autosampler is Connected

Parameter Set To:
Remote Start In High to Low
Inhibit Ready In Neither
End of Run Out High to Low
Start of Run Out High to Low
GC Ready Out When Low
Prep Run Out When Low

 To configure handshaking parameters through the touch screen

1. In the main menu select the Configuration icon, the Configuration menu appears.
2. In the Configuration menu, select the Handshake icon to open the relevant submenu.
3. Set the Handshaking parameters as required, then return to main menu.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 41

1 Installation
Installing the Data System Software

 To configure handshaking parameters through the Chromatography Data System

1. Launch the Data System. In the relevant Configuration page specify the handshaking
parameters.

Installing the Data System Software

The data systems as Chromeleon, Xcalibur, Chrom-Card, or ChromQuest, are designed to be
compatible with commercially available computers and requiring the use of Windows™ XP,
Windows™ Vista, or Windows™ 7 operating system.

IMPORTANT Before installing the Data System, please make sure that any previous
version is removed from the PC hard drive.

 To install the data system software

1. Remove the current version.

a. Select Control Panel > Add/Remove Programs.
b. In the dialog window displayed, select the current Data System software version to
remove.
c. Click Add/Remove.
2. Install the new version.
a. Insert the CD/DVD provided and start Setup.exe. Click Next several times.
b. Continue the installation by following the instructions displayed.
c. At the end of the installation, reboot the computer if required.
d. Start the Data System you installed selecting it in the page Start-Program of the
desktop.
e. The Main Page of the data system will be displayed.
For details about the data system in use, please refer to the relevant User Guide.

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 1 Installation
Making Power Connections

Making Power Connections

INSTRUMENT DAMAGE
A label on the electronic module indicates the 120 Vac or 230 Vac power supply. If your
power supply line does not match the power supply required, DO NOT CONNECT
AND DO NOT POWER ON THE GC.

IMPORTANT The external modules must be powered at the same line voltage of the main
GC system.

 To connect the power to the GC

1. Connect the power cable to the AC Input connector (Main socket) on the GC, and to the
wall outlet. See Figure 51.

Figure 51. GC Power Connection

120 Vac or 230 Vac Power

Supply Label

Power Switch ON Position

AC Input Connector

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1 Installation
Making Power Connections

2. If external modules as Aux Temperature/Cryo Modules, NPD Thermionic Source

Power Module, or both are present, connect the power cable to the AC Input connector
on the front of each external module, and to the wall outlet. See Figure 52.

IMPORTANT The external modules must be powered at the same line voltage of the main
GC system.

Figure 52. External Module Power Connection

Aux Temperature/Cryo Module

AC Input Connector

NPD Thermionic Source Power Module

AC Input Connector

• Aux Temperature/Cryo Module — The LED marked On lights up solid green after
the GC power on.
• NPD Thermionic Source Power Module — The LED marked On blinks green at
the plug-in of the power cable, and becomes solid green at the power on of the
thermionic source.

Note The modules Auxiliary Gas, Generic Detector Interface, and Analog Outputs
Interface, receive the power supply from the electronic module of the GC through the
GC Bus connection.

Auxiliary Gas Module

Generic Detector Interface: Electrical Interface

Analog Output Interface Module

The LED marked On lights up solid green after the GC power on.

44 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Setting the LAN Communication

Setting the LAN Communication

This section provides instructions for setting the desired IP and the LAN communication port
for the TRACE 1300/TRACE 1310. See “Making the LAN Setup” on page 46.
Before making the LAN setup, please read the following note.

Note The TRACE 1300/TRACE 1310 allows LAN (Local Area Network) capabilities by
the presence of an RJ45 connector on the Electronic Module, and two LEDs for LAN
activity.
• IP Address —The GC is shipped with a factory IP address, which may not match the
LAN needs of the sites where the instrument will be installed. To change the default
values, contact your LAN administrator and ask for the IP address to be assigned, the
netmask, and eventually the port.
– The IP address is a 3 digits x 4 fields number given by the network administrator
- e.g. 192.168.127.10
– The netmask is a 3 digits x 4 fields number given by the network administrator -
e.g. 255.255.255.0
– The port is a number given by the network administrator - for example 2551
• Reset Button — To reset the LAN parameters (IP address, communication port, and
so on) to the default values, insert a small screwdriver into the hole, then push the
reset button for at least five seconds.
• Network Cables — Two network cables are included in the standard outfit:
– a reversed RED patch for the computer to TRACE 1300/TRACE 1310 direct
connection. This is used for the initial setup operation and when the instrument
is not connected in remote mode.
– a standard GREY patch for the TRACE 1300/TRACE 1310 to local area
network connection.
Figure 53 shows the LAN schematic connection between the TRACE 1300/TRACE
1310 and the PC.

Figure 53. LAN Schematic Connections

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1 Installation
Setting the LAN Communication

Making the LAN Setup

 To set the LAN communication

1. Using the LAN RED patch, connect a desktop or laptop PC directly to the RJ45
connector marked LAN/Ethernet on the Electronic Module of the GC. See Figure 54.

Figure 54. GC/PC LAN Connection

Computer

LAN Standard RED Patch

2. Power on the GC by placing the power switch in the On (up) position marked I.
3. Power on the PC.
4. Start the TS1300ST program to begin the installation on the PC. Follow the instructions
step by step to complete the installation.

Note The TS1300ST program is used for setting the LAN Communication parameters,
and for updating the Firmware version on the GC.

Updating the Firmware version on the GC must be carried out by a Thermo Fisher
Scientific authorized technical personnel.

Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to

contact your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer
Support

5. Run the TS1300ST program; the initial page appears and you will be prompted to enter
the password. See Figure 55.

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 1 Installation
Setting the LAN Communication

Figure 55. Initial Page and Password

6. In the text box enter the password ThermoFisher, then press OK.
7. The system starts scanning the equipment powered on and connected to the LAN.
During this stage, the three buttons on the top of the page are disabled. See Figure 56.

Figure 56. Research of the Equipment

8. At the end of the scan, all the equipment powered on and connected to the LAN is
displayed. Only the SCAN button is enabled. See the example in Figure 57.

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1 Installation
Setting the LAN Communication

Figure 57. Equipment Connected to the LAN

Note To repeat the scan, click SCAN.

9. Select the GC of interest to set up. All the three buttons on the top of the page are
enabled. See the example in Figure 58.

Figure 58. Selection of the GC of Interest

10. Click IP set-up. The Remote Settings page is displayed. See the example in Figure 59.

48 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Setting the LAN Communication

Figure 59. Remote Settings Page

The editable parameters are: Description, DHCP, IP, Netmask, and Gateway.
After changing parameters, press OK to confirm the new settings.
11. Close the TS1300ST program by pressing on the right upper corner of the page, or
selecting the command Close in the drop-down menu. See Figure 60.

Figure 60. Close Commands

12. Configure the Data System (Xcalibur, Chrom-Card, ChromQuest, or Chromeleon) in

use. See the section “Configuring the Data System” on page 50.

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1 Installation
Column Installation Requirements

Configuring the Data System

You should use the self adhesive labels found in the standard outfit of the Data System to
annotate the IP address and the TCP Port that are set in the instrument. Stick the label where
it can be easily found when necessary to configure the data system.
1. Launch the Data System. In the relevant Configuration page specify a direct TCP/IP
address.
2. Enter the IP address of the GC and set the socket used.
Standard operation parameters are set by default; however, your GC may be hidden
behind a Firewall that may prevent Port 2551 from being used.
You can set an alternative port number. The number of the socket entered in this box
must correspond to the port assigned to the instrument setup.

IMPORTANT If the connection is performed through hubs over a 10 Mbit/s network, it is

suggested that no more than five GC units be connected on the same network trunk.
If you have a switched network, this warning does not apply.

Column Installation Requirements

The column is where the separation takes place. It should be positioned inside the oven on the
column rack. The column ends should align correctly with the injector and the detector
bottom fittings. The GC oven controller accurately controls the column temperature.
Each column has a maximum recommended operating temperature. To protect the column
from excessively high temperatures, remember to set the Maximum temperature parameter
for the oven.
Note For detailed information on maximum operating temperature, refer to the column
manufacturer’s instructions provided with the column.

Related Topics
• “Using the Correct Fittings” on page 50
• “Installing the Adapters for Encapsulated Graphite Ferrules” on page 52
• “Installing the Column Rack” on page 56

Using the Correct Fittings

To connect a capillary column to the injector and detector base body, you must use the proper
column ferrules and retaining nuts.
• Retaining Nut — Dedicated retaining nuts are required to connect capillary columns to
injector and detector base bodies, and the Tee connector. See Table 4.

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 1 Installation
Column Installation Requirements

Table 4. Column Retaining Nuts for Injectors and Detectors

Retaining Nut Type Injector/Tee Connector Detector
1/4” Hexagonal SSL, SSLBKF FID, NPD, TCD,
retaining nut ECD, FPD

M4 Split retaining PTV, PTVBKF,

nut SSLBKF
(Tee Connector)

• Ferrules — Graphite ferrules, graphite Vespel® ferrules, and encapsulated graphite

ferrules are used for many column connections. See Table 5.
Table 5. Ferrules for Injectors and Detectors
Injector/Tee Temperature
Ferrule Type Detector
Connector Limit
Graphite ferrule SSL, SSLBKF FID, NPD, TCD, 450 °C
ECD, FPD

Graphite Vespel® SSL, SSLBKF FID, NPD, TCD, 350 °C

ferrule ECD, FPD

Encapsulated PTV, PTVBKF, 450 °C

graphite ferrule SSLBKF
(Graphpack) (Tee Connector)
See the Note
below.
Graphite ferrules are a soft material that is porous to oxygen, making them suitable for most
applications except GC/MS interface connections. These easy-to-use ferrules form a soft grip with the
column and provide a stable seal. Graphite ferrule are re-usable.

Graphite/Vespel® ferrules have a long lifetime and are compatible with GC/MS. These ferrules form
a strong grip with the column and cannot be reused as they form a permanent seal with the column.
They have a temperature limit of 350 °C, but must be re-tightened after initial temperature cycles.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 51

1 Installation
Column Installation Requirements

Note An optional adapter kit must be installed if you want to connect the column to
the injector and detector by using the encapsulated graphite ferrule. See “Installing
the Adapters for Encapsulated Graphite Ferrules” on page 52.

Table 6, Table 7, and Table 8 list the size of the ferrule to use depending on the internal
diameter of precolumn and capillary column.
Table 6. Graphite Ferrules Size ID
Ferrules Size ID Required for:
0.4 mm 0.1 - 0.32 mm ID capillary column
0.8 mm 0.45 - 0.53 mm ID capillary column

Table 7. Graphite Vespel Ferrules Size ID

Ferrules Size ID Required for:
0.4 mm 0.1/0.2/0.25 mm ID capillary column
0.5 mm 0.32 mm ID capillary column
0.8 mm 0.53 mm ID capillary column

Table 8. Encapsulated Graphite Ferrules Size ID

Ferrules Size ID Required for:
0.4 mm 0.25 mm ID capillary column
0.5 mm 0.32 mm ID capillary column
0.8 mm 0.45/0.53 mm ID capillary column

CAUTION Using the wrong size ferrules causes leaks and contamination.

When connecting columns, consider that overtightening compression ferrules does not
necessarily produce a stronger, leak-free joint. In fact, very often the reverse is true. Too
much pressure can cause a leak in the joint and make it very difficult to reseal the joint
when changing columns.

Installing the Adapters for Encapsulated Graphite Ferrules

This section provides instructions for installing the optional adapters for connecting the
capillary column to the SSL injector and FID, NPD, TCD, ECD, and FPD detector by using
the encapsulated graphite ferrules (Graph-pack).
The adapters are provided with the kit PN 19050759. The kit includes the parts listed in
Table 9.

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 1 Installation
Column Installation Requirements

Table 9. Adapters and 1/2-in. Wrench

Part Description
Adapter for connection to the SSL injector

Adapter for connection to the FID, NPD, ECD, TCD, or

FPD detector

1/2-in. wrench

 To install the adapters for encapsulated graphite ferrules

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Remove the analytical column.

a. Open the front door of the GC.
b. Loosen the retaining nut from the injector and detector fitting on the upper interior
wall of the GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector and the detector.
5. Remove the bottom parts of the SSL injector. See Figure 61.

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1 Installation
Column Installation Requirements

Figure 61. SSL Injector Bottom Parts Removal

SSL Injector Bottom

Base Seal
Washer

Retaining Nut

a. Using the 1/2-in. wrench provided, unscrew the retaining nut with the base seal and
the washer from the bottom of the injector.

CAUTION Make sure that the liner does not come from the bottom of the injector.

Save the bottom parts of the SSL injector in a safe place because will be reused when you
restore the original configuration.

6. Install the adapter on the bottom the injector. See Figure 62.

Figure 62. Adapter Installation on SSL Injector Bottom

Silver Washer

Graphpacl SSL
Bottom

SSL Washer
Inlet

Column Adapter
Nut

Retaining Nut

a. Fingertighten the retaining nut of the adapter until it start to grip the bottom of the
injector.
b. Use the 1/2-in. wrench to firmly tighten the retaining nut of the adapter with the
base seal and the washer from the bottom of the injector.
7. Remove the bottom parts of the FID, NPD, ECD, TCD, or FPD detector. See Figure 63.

54 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Column Installation Requirements

Figure 63. Detector Bottom Parts Removal

Detector Bottom

Retaining Nut

Note Save the bottom parts of the detector in a safe place because they will be reused
when you restore the original configuration.

a. Using the 1/2-in. wrench provided, unscrew the retaining nut from the bottom of the
detector.
8. Install the adapter on the bottom the detector. See Figure 64.

Figure 64. Adapter Installation on the Bottom of the Detector

Silver Gasket

FID Graphpack
Adapter

a. Fingertighten the retaining nut of the adapter until it starts to grip the bottom of the
detector.
b. Use the 1/2-in. wrench to firmly tighten the retaining nut of the adapter with the
base seal and the washer from the bottom of the injector.

9. Connect the column.

a. Before starting, use a 1/4-5/16-in. wrench to remove the column nuts and ferrules
from the base of the injector and detector adapters. See Figure 65.

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1 Installation
Column Installation Requirements

Figure 65. Column Nut and Ferrules

SSL Injector

Ferrules

Column Nut

Detector

Ferrules

Column Nut

b. Continue the installation of the capillary column following the instructions in the
section “Installing the Column the First Time” on page 57.

Installing the Column Rack

WARNING Before starting the installation, make sure that the GC is powered off and the
power cable is disconnected.

Before connecting the capillary column, make sure that the column rack is installed in the
oven. See Figure 66.

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 1 Installation
Installing the Column the First Time

Figure 66. Column Rack

Button-holes

Hooks
Oven Heater
Baffle
Arms

Column Rack

 To install a column rack

1. Open the front door of the GC.

2. Slightly press the sides of the column rack and insert the two hooks into the
corresponding button-holes on the oven heater baffle.

Installing the Column the First Time

This section contains instruction for installing the capillary column into the GC oven, for
connecting the column ends to the injector and the detector and for carrying out the column
conditioning, the leak test and the column evaluation.

CAUTION The injector and detector modules are shipped with a plug.

Before installing the column, remove the plug from the injector and detector modules.

Unscrew the column retaining nut from the bottom of the injector and the detector
modules, then remove the blind ferrule.

 To install a new column the first time

1. Install the column into the GC oven.

a. Open the front door of the GC.
b. Place the column on the two arms of the rack. See Figure 67.

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1 Installation
Installing the Column the First Time

Figure 67. Column Installation

2. Connect the column to the injector inside the oven.

a. Unwind the column enough to easily connect its ends to the injector and the
detector.
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.

b. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
c. Insert the column through the injector retaining nut and the proper ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column about 1 cm (0.4 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and end of the
column.

e. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in Table 10.

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 1 Installation
Installing the Column the First Time

Table 10. Column Insertion Depth For SSL, SSLBKF, HeS-S/SL, PTV, PTVBKF, and GSV
Injectors
Injector Column Insertion Depth
SSL and HeS-S/SL • 5 mm (splitless)
• 5 mm (Helium saver)
• 10 mm (split)
SSLBKF • 5 mm (splitless)
• 10 mm (split)
PTV • 30 mm
• As far as possible into the bottom
when the PTV is used as an
On-Column injector.
PTVBKF • 30 mm
GSV • Insert the column as far as goes and
withdrawn about 2 -3 mm

f. Insert the notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.
g. Adjust the column position so that the septum contact the bottom of the retaining
nut.
h. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
i. Remove the notched septum from the column.
3. Open the gas supplies.
4. Power on the GC.
a. Connect the power cable to the AC Input connector on the back of the GC and to
the wall outlet.
b. Flip up the power switch (breaker) to the position I.
5. Setup the GC.
a. Set the oven and injector temperature to 50 °C.
b. Use the column-flowmeter connector to verify that there is flow through the column.
If you do not have a flowmeter, dip the column outlet in a small vial of methanol.
Bubbles indicate there is flow through the column. If there is no flow, check that the
carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is
still no flow, consult the section Analytical Troubleshooting in the TRACE
1300/TRACE 1310 User Guide, or contact the Technical Support.
c. Allow the column to purge for few minutes.
6. Perform a column leak check.

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1 Installation
Installing the Column the First Time

a. Carefully push the capillary column end into the column section of the
column-flowmeter connector. See Figure 68.
Figure 68. Using a Flowmeter for Leak Check
A B C

b. If your GC is equipped with the touch screen as user interface, select the Leak Check
icon in the Maintenance menu, otherwise perform the Leak Check through the
Chromatography Data System by selecting the proper function.
c. Start the leak check to begin operation. The split and purge valves of the selected
channel are automatically closed and the channel is pressurized with carrier gas to the
leak check setpoint.
d. The system monitors the pressure for one minute. If the pressure does not drop more
than the maximum allowed sensitivity value, then the leak check will pass.
e. If the leak check did not pass, you should use the leak detector to find and fix the
leaks.

Tip Leaks can be caused by not tightening the fitting on the column-flowmeter
connector. We recommend that you check that fitting before looking elsewhere.

f. Repeat the leak check until no leaks are indicated.

7. Calibrate the carrier gas flow (column evaluation).
a. Carefully push the capillary column end into the flow meter section of the column-
flowmeter connector. See Figure 69.

Figure 69. Column Flowmeter Connector

A B C

To flowmeter

60 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 1 Installation
Installing the Column the First Time

b. Connect the flowmeter to the dedicated fitting on the column-flowmeter connector.

c. If your GC is equipped with the touch screen as user interface, select Back or Front
Column icon in the Configuration menu, otherwise perform the Column
Evaluation through the Chromatography Data System by selecting the proper
function.
d. Select the column and input the physical characteristics of the column.
e. If a pre-/post-column is present, set the length and nominal internal diameter of the
pre-/post-column in the same valid ranges for the column. The following two lines
are added to the menu.
f. According to the physical characteristics of the column, the system calculates and
displays the relevant Column K-factor.

Note For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.

g. Start column evaluation. At the end of the routine, a message will indicate that
evaluation was successful.
h. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm ID column
(1.3 – 2.0 for a 30 m, 0.25 mm ID column). If the column does not report a K-factor
within this range or within 0.1 units of the previous stored value, check for a leak or
broken column using the leak detector. The K-factor is a measured resistance for the
column. A K-factor that is too low may indicate a leak in the system, while a K-factor
that is too high may indicate a blockage.
8. Disconnect the column-flowmeter.
a. Disconnect the column from the column-flowmeter connector.
b. Remove the clear plastic component, including its fittings, from the oven and set it
aside.
c. Close the GC door.
9. Condition the column.
The column must be conditioned before inserting it into the detector.
Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the column manufacturer’s instructions.
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.

CAUTION When performing column conditioning, the column should be connected only
to the injector leaving the column outlet disconnected to avoid the possibility of
contamination of the detector. Do not use hydrogen as the carrier for conditioning! It
could vent into the oven and present an explosion hazard.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 61

 a. Run the temperature program that is recommended by the manufacturer.

INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating

temperature.

10. Connect the column to the detector inside the GC.

a. Lower the oven temperature to 30 °C and allow it to cool.

WARNING-BURN HAZARD: The injector, detector, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.

b. Unwind the column enough to easily connect its ends to the injector and the
detector..
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.

c. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column outlet about 2.5cm (1 in.) from
the end. Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert the column through the proper detector retaining nut and ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
Tip Slide a notched septum on the column before the detector retaining nut to
make it easier to measure the proper distance between the bottom nut and end of
the column.

f. For FID, NPD, TCD, ECD, and FPD, position the column so that the end of the
column extends the proper distance above the end of the ferrule as reported in
Table 11. For PDD see the instruction described at the step g on page 63.
Table 11. Column Insertion Depth For FID, NPD, TCD, ECD, FPD, and PDD Detectors
Detector Column Insertion Depth
FID, NPD, and TCD Insert the column as far as goes and
withdrawn about 2 -3 mm
ECD 23 mm
FPD 125 mm
PDD 136 mm

i. For FID, NPD, and TCD, insert the column into the detector, paying attention
to not force it further. Finger-tighten the retaining nut, then withdraw the
column 2-3 mm. Tighten the retaining nut an additional a quarter turn.
 1 Installation
Installing the Column the First Time

ii. For ECD and FPD, insert the notched septum on the column to hold the
retaining nut in this position. Thread the retaining nut into the detector but do
not tighten. Adjust the column position so that the septum contact the bottom
of the retaining nut.
Finger-tighten the retaining nut until it starts to grip the column plus a quarter
turn.
iii. Remove the notched septum from the column.
g. For PDD the capillary column must enter 136 mm into the pre-installed capillary
column adapter.
i. Make a mark on the column 136 mm from the end.
ii. Remove the knurled nut column inlet at the bottom of the detector. Slide the nut
overt the end of the column, followed by the appropriate column ferrule.
iii. Seat the ferrule in the detail of the column adapter and begin sliding the column
through the capillary column adapter and into the column inlet.
iv. Get the nut started on the threads and tighten it until you feel it contact the
ferrule, then back off half a turn.
v. Slide the column into the column inlet until the mark is flush with the surface of
the knurled nut, and secure the column in the adapter by tightening the knurled
nut finger tight only.

Note When inserting the capillary column into the PDD detector it might rarely
happen to feel a slight resistance. In this case, for proper column installation, pull
the column out slightly and adjust the angle before inserting it further.

IMPORTANT To install a packed column, the pre-installed capillary column adapter must
be replaced with the packed columns adapter that enters into the PDD cell for the correct
length.

11. End of the column installation.

a. Close the front door of the GC.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 63

 2

Performing Routine Maintenance

This chapter provides instructions for performing routine maintenance on the TRACE 1300
and TRACE 1310.

Contents
• Read Me First
• Maintenance Supplies and Tools
• Maintenance Button
• Powering On the TRACE 1300/TRACE 1310
• Shutting Down the TRACE 1300/TRACE 1310
• Cleaning the Instrument Externally
• Replacing a Column

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 65

2 Performing Routine Maintenance
Read Me First

Read Me First
The instrument will be generally serviced by Thermo Fisher Scientific authorized technical
personnel for all the warranty period or, after warranty, possibly according to a Programmed
Service Contract. For more information contact your local Thermo Fisher Scientific office.

WARNING If, for technical reasons, it is necessary to work on parts of the machine that
may involve hazardous operations (moving parts, components under voltage, and so
on).Thermo Fisher Scientific authorized Technical Support must be called.

This situation can be identified because the access to these moving parts is possible only
using a particular tool, and because the concerned removable protective covers bear a
warning symbol that draws the operator’s attention to the specific warnings included in
the documentation accompanying the instrument. In case the work must be carried out
by the operator, the latter must prove to be adequately trained to perform the specific
maintenance operation.

There are only a few TRACE 1300/TRACE 1310 components that require routine
maintenance, depending on the quantity and types of samples you are running. A frequently
used instrument will, of course, require more maintenance than an instrument that is rarely
used.
• External Cleaning — The GC needs to be cleaned when it gets dirty. See the section
“Cleaning the Instrument Externally” on page 72.
• Column — You may need to replace the column when your performance degrades and
troubleshooting indicates that the column needs maintenance. That may mean that end
of the column needs to be trimmed or the column needs to be replaced.
See “Replacing a Column” on page 73.
• Injector modules — You may need to install, replace or maintain an injector module.
See Chapter 3, “Performing Injectors Routine Maintenance.”
• Detector modules — You may need to install, replace or maintain a detector module.
See Chapter 4, “Performing Detectors Routine Maintenance.”

There are many more components in the TRACE 1300/TRACE 1310 that do not require
routine maintenance, but may need to be replaced if there is a problem with the instrument.
To replace any component not listed in this chapter, see the following chapters:
• Chapter 5, “GC Main Frame Advanced Maintenance.”
• Chapter 6, “Injectors Advanced Maintenance.”
• Chapter 7, “Detectors Advanced Maintenance.”

66 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 2 Performing Routine Maintenance
Read Me First

IMPORTANT
All the maintenance operations must be carried out at low temperature to avoid burns.
Therefore, before beginning the sequence, the GC oven, injectors and detectors must be
cooled to room temperature, and then the gases supply must be closed.

You can carry out these operations manually, or by pressing the Maintenance key. For
details refer to the TRACE 1300 and TRACE 1310 User Guide.

At the end of the maintenance operations, restore the GC normal working conditions.

Maintenance of an injector

Before opening the injector for maintenance, turn the carrier gas off, and wait for the
carrier pressure to go to zero.

When an autosampler is present:

Move the autosampler away from the injector module to create free space around it:
• If an AI 1310/AS 1310 - AI 3000/AS 3000 II is installed, pull the sampler support
plate outwards.
• If a TriPlus RSH, TriPlus 100 Liquid Sampler, or TriPlus is installed, make sure that
the standby turret position does not obstacle the injector or detector module to
maintain. If yes, change the position of the turret/head.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 67

2 Performing Routine Maintenance
Maintenance Supplies and Tools

Maintenance Supplies and Tools

To perform routine maintenance on the TRACE 1300/TRACE 1310, you will need the
following supplies and tools.
• Wrench, open-end, 1/4-in. and 5/16-in., 1/8-in.
• Flathead screwdriver
• 5.5 x 25 mm Slotted Stubby Driver
• Column cutter, wafer (5181-8836, 4/pk)
• T20 Torxhead screwdriver
• T10 Torxhead screwdriver
• T6 Torxhead screwdriver
• 3-mm Allen key wrench
• Electronic flowmeter (Thermo Scientific GFM Pro Flowmeter, or equivalent)
• Electronic leak detector (Thermo Scientific GLD Pro, or equivalent)
• Tweezers (or thin needle-nose pliers) or forceps
• Gloves, heat-resistant (for handling hot parts)

Cleaning Stainless Steel Components

To clean stainless steel components, you will need:
• Acetone, reagent grade (or other suitable polar solvent)
• Applicators, cotton-tipped
• De-ionized water
• Detergent (Alconox™, Micro®, or equivalent)
• Gas, clean and dry (N2 or He)
• Gloves, clean, lint- and powder-free, latex or nitrile
• Lint-free cloth
• Ultrasonic cleaner

68 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 2 Performing Routine Maintenance
Maintenance Button

Maintenance Button
When pressed, the Maintenance button allows cooling at 60 °C of the selected heated zones,
which allows the required maintenance. Carrier and detector gases must be closed manually.
This function also performs the following actions automatically:
• turns off the flame and the fuel gases of the FID and FPD detectors.
• turns off the thermionic source, the hydrogen, and the air flows of the NPD detector.
• turns off the filaments of the TCD detector.

Powering On the TRACE 1300/TRACE 1310

 To power on the GC

1. Install the GC column (see “Replacing a Column” on page 73).

2. Open the supply gases.
3. Plug the power cable to the AC Input connector (Main socket) on the GC, and to the
wall outlet. See Figure 70.
4. Be sure the carrier gas flowing through the column and the detector gases flowing
through the detector.

Figure 70. GC Power On

120 Vac or 230 Vac

Power Supply Label

Power Switch ON Position

AC Input Connector

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 69

2 Performing Routine Maintenance
Powering On the TRACE 1300/TRACE 1310

5. If external modules are present, plug the power cable to the AC Input connector of each
module, and to the wall outlet.
6. Flip up the power switch, located on the back side of the GC, to the On (up) position
marked I.
a. TRACE 1300 — When the GC powers on, all the LEDs on the status panel light up
simultaneously, afterward the Power light becomes a solid green while all the other
lights turn off. The GC is now in stand-by status. See Figure 71.

Figure 71. TRACE 1300 Status Panel at the GC Power On

.

Power On Status

Stand-by Status

b. TRACE 1310 — Check the main menu appears on the touch screen. See Figure 72.

Figure 72. TRACE 1310 Touch Screen

7. Open the Thermo Scientific Chromatography Data System installed on the computer.
8. Set the analytical parameters.

70 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 2 Performing Routine Maintenance
Shutting Down the TRACE 1300/TRACE 1310

Shutting Down the TRACE 1300/TRACE 1310

 To shut down the GC

1. Cool down the GC.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

2. If you do not plan to replace the column or perform maintenance on the GC, you do not
have to lower the injector temperature.
3. Turn off the carrier gas supply at the tank.
4. Push down the power switch (breaker), located at the back of the instrument, to the
position O (down). See Figure 73.

Figure 73. GC Power Off

Power Switch Off Position

AC Input Connector

5. If external modules are present, unplug the power cable from the AC Input connector of
each module, and from the wall outlet.
6. If present, power-off the autosampler by switching off the main power switch, or by
unplugging the power cable from the AC input connector, and from the wall outlet.
7. Power-off all the remaining instruments.

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2 Performing Routine Maintenance
Cleaning the Instrument Externally

Cleaning the Instrument Externally

Normal usage of the TRACE 1300/TRACE 1310 can cause the exterior to get dirty.

WARNING It is your responsibility to avoid that dangerous liquids, materials or both,

seeping inside the GC during operation and maintenance.

Solvent must not be used. Do not spray on electrical parts.

 To clean the instrument externally

1. Place the GC in stand-by condition.

2. Press the Maintenance button to cool down the GC, or switch off the heated zones
manually.
3. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector on the back of the GC, and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each module, and from the wall outlet.
5. Externally clean the instrument with a soap and water solution, or with a household
non-abrasive product.
• Pay special attention when cleaning the back side of the instrument. Do not spray,
but clean using a cloth imbued with the same substance.
• Carefully avoid seeping of the products used inside the instrument, particularly when
cleaning the grid of the back panel.
• If you just suspect that a substance used for cleaning or a product submitted to
analysis has penetrated inside the instrument, immediately shut down the
instrument, and call an authorized customer support engineer for proper actions.
The service engineer must be fully informed on the nature of the concerned
substance.
In the event that a hazardous material is spilled on or in the instrument, clean the
spill according to the procedures reported in the Material Data Sheet for that
substance.
6. Dry with a clean cloth.
7. If external modules are present, plug the power cable to the AC Input connector of each
module, and to the wall outlet.
8. Power on the GC.

72 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 2 Performing Routine Maintenance
Replacing a Column

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
c. Set the normal injector, detector and GC working conditions.

Replacing a Column
You might need to replace the column when your performance degrades and troubleshooting
indicates that the column needs maintenance. That might mean that the end of the column
needs to be trimmed, or the column needs to be replaced.

WARNING-BURN HAZARD: The injector and the oven could be hot. Cool to room
temperature before touching them.

 To replace a column

Note If you are running samples, stop the acquisition.

1. Remove the current column.

a. Press the Maintenance button to cool down the GC.
b. Set off the carrier and detector gases of the channel of interest, and wait for the carrier
pressure to go to zero.
c. Open the front door of the GC.
d. Unscrew the injector and detector nuts, and remove the column.
e. Remove the column from the column rack, and from the GC.
2. Install the new column.
a. Place the new column on the two arms of the column rack inside the oven.
3. Connect the new column to the injector inside the GC.
a. Unwind the column enough to easily connect its ends to the injector and the
detector..
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.

b. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
c. Insert the column through the proper injector retaining nut and ferrule (open end
up). If the M4 retaining nut is used, slide it on the column through the side cut.
Wipe the column again with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column about 1 cm (0.4 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 73

2 Performing Routine Maintenance
Replacing a Column

Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and end of the
column.

e. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in Table 12.
Table 12. Column Insertion Depth For SSL, SSLBKF, HeS-S/SL, PTV, PTVBKF, and GSV
Injectors
Injector Column Insertion Depth
SSL and HeS-S/SL • 5 mm (splitless)
• 5 mm (He saver
• 10 mm (split)
SSLBKF • 5 mm (splitless)
• 10 mm (split)
PTV • 30 mm
• As far as possible into the bottom
when the PTV is used as an
On-Column injector.
PTVBKF • 30 mm
GSV • Insert the column as far as goes and
withdrawn about 2 -3 mm

f. Insert the notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.
g. Adjust the column position so that the septum contact the bottom of the retaining
nut.
h. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
i. Remove the notched septum from the column.
4. Setup the GC parameters.
a. Set the oven and injector temperature to 50 °C.
b. Use the column flowmeter connector to verify that there is flow through the column.
If you do not have a flowmeter, dip the column outlet in a small vial of methanol.
Bubbles indicate there is flow through the column. If there is no flow, check that the
carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is
still no flow, consult the Troubleshooting section or contact Technical Support.
c. Allow the column to purge for at least 10 minutes.
5. Perform a column leak check.

74 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 2 Performing Routine Maintenance
Replacing a Column

a. If your GC is equipped with the touch screen as user interface, select the Leak Check
icon in the Maintenance menu, otherwise perform the Leak Check through the
Chromatography Data System by selecting the proper function.
b. Start the leak check to begin operation. The split and purge valves of the selected
channel are automatically closed and the channel is pressurized with carrier gas to the
leak check setpoint.
c. The system monitors the pressure for one minute. If the pressure does not drop more
than the maximum allowed sensitivity value, then the leak check will pass.
If the leak check did not pass, you should use the leak detector to find and fix the
leaks.

Tip Leaks can be caused by not tightening the fitting on the column flowmeter
connector. We recommend that you check that fitting before looking elsewhere.

d. Repeat the leak check until no leaks are indicated.

6. Calibrate the carrier gas flow (column evaluation).
a. Carefully push the capillary column end into the flow meter section of the column
flowmeter connector. See Figure 74.

Figure 74. Column Flowmeter Connector

A B C

To flowmeter

b. Connect the flowmeter to the dedicated fitting on the column flow meter connector.
c. If your GC is equipped with the touch screen as user interface, select Back or Front
Column icon in the Configuration menu, otherwise perform the Column
Evaluation through the Chromatography Data System by selecting the proper
function.
d. Select the column and input the physical characteristics of the column.
e. If a pre-/post-column is present, set the length and nominal internal diameter of the
pre-/post-column in the same valid ranges for the column. The following two lines
are added to the menu.
f. According to the physical characteristics of the column, the system calculates and
displays the relevant Column K-factor.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 75

2 Performing Routine Maintenance
Replacing a Column

Note For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.

g. Start column evaluation. At the end of the routine, a message will indicate that
evaluation was successful.
h. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm ID column
(1.3 – 2.0 for a 30 m, 0.25 mm ID column). If the column does not report a K-factor
within this range or within 0.1 units of the previous stored value, check for a leak or
broken column using the leak detector. The K-factor is a measured resistance for the
column. A K-factor that is too low may indicate a leak in the system, while a K-factor
that is too high may indicate a blockage.
7. Disconnect the column flowmeter.
a. Disconnect the column from the column flowmeter connector.
b. Remove the clear plastic component, including its fittings, from the oven and set it
aside.
c. Close the GC door.
8. Condition the column.
The column must be conditioned before inserting it into the detector.
Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the column manufacturer’s instructions.
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.

CAUTION When performing column conditioning, connect the column only to the
injector leaving the column outlet disconnected to avoid the possibility of contamination
of the detector.

Do not use hydrogen as the carrier for conditioning! It could vent into the oven and
present an explosion hazard.

a. Run the slow temperature program that is recommended by the manufacturer.

INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating

temperature.

9. Connect the column to the detector inside the GC.

a. Lower the oven temperature to 30 °C and allow it to cool.

76 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 2 Performing Routine Maintenance
Replacing a Column

WARNING-BURN HAZARD: The injector, detectors, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.

b. Unwind the column enough to easily connect its ends to the injector and the
detector.

Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.

c. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column outlet about 2.5cm (1 in.) from
the end. Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert the column through the proper detector retaining nut and ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
Tip Slide a notched septum on the column before the detector retaining nut to
make it easier to measure the proper distance between the bottom nut and end of
the column.

f. For FID, NPD, TCD, ECD, and FPD, position the column so that the end of the
column extends the proper distance above the end of the ferrule as reported in
Table 13. For PDD see the instruction described at the step g on page 77.
Table 13. Column Insertion Depth For FID, NPD, TCD, ECD, FPD, and PDD Detectors
Detector Column Insertion Depth
FID, NPD, and TCD Insert the column as far as goes and
withdrawn about 2 -3 mm
ECD 23 mm
FPD 125 mm
PDD 136 mm

i. For FID, NPD, and TCD, insert the column into the detector, paying attention
to not force it further. Finger-tighten the retaining nut, then withdraw the
column 2-3 mm. Tighten the retaining nut an additional a quarter turn.
ii. For ECD and FPD, insert the notched septum on the column to hold the
retaining nut in this position. Thread the retaining nut into the detector but do
not tighten. Adjust the column position so that the septum contact the bottom
of the retaining nut. Finger-tighten the retaining nut until it starts to grip the
column plus a quarter turn.
iii. Remove the notched septum from the column.
g. For PDD the column must enter 136 mm into the pre-installed capillary column
adapter.
i. Make a mark on the column 136 mm from the end.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 77

2 Performing Routine Maintenance
Replacing a Column

ii. Remove the knurled nut column inlet at the bottom of the detector. Slide the nut
overt the end of the column, followed by the appropriate column ferrule.
iii. Seat the ferrule in the detail of the column adapter and begin sliding the column
through the capillary column adapter and into the column inlet.
iv. Get the nut started on the threads and tighten it until you feel it contact the
ferrule, then back off half a turn.
v. Slide the column into the column inlet until the mark is flush with the surface of
the knurled nut, and secure the column in the adapter by tightening the knurled
nut finger tight only.

Note When inserting the capillary column into the PDD detector it might rarely
happen to feel a slight resistance. In this case, for proper column installation, pull
the column out slightly and adjust the angle before inserting it further.

IMPORTANT To install a packed column, the pre-installed capillary column adapter must
be replaced with the packed columns adapter that enters into the PDD cell for the correct
length.

10. End of the column installation.

a. Close the front door of the GC.

78 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific

 3

Performing Injectors Routine Maintenance

This chapter provides instructions for performing routine maintenance on the TRACE 1300
and TRACE 1310 injector modules.

Contents
• Maintaining a Split/Splitless Injector (SSL)
• Maintaining a Split/Splitless Injector with Backflush (SSLBKF)
• Maintaining a Gas Sampling Valve Injector (GSV)
• Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)
• Maintaining a Programmable Temperature Vaporizing Injector (PTV)
• Maintaining a Programmable Temperature Vaporizing Injector with
Backflush (PTVBKF)

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

Maintaining a Split/Splitless Injector (SSL)

This section provides instructions for maintaining a Split/Splitless injector (SSL).

The module and injector components are shown in Figure 75 and Figure 76.

Figure 75. SSL Module Components

Split Line Vent

Purge Line Vent

Septum Cap

Septum Holder/Liner Cap

Ring Nut

Carrier Gas Line Filter

Injector Body

Split Gas Line Filter

Insulation Cover

Bottom Fittings

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

Figure 76. SSL Injector: Components

Septum Cap

Septum

Ring Nut

Septum Holder/Liner Cap

Liner Seal
Liner
Body Head External O-Ring

Body Head Internal O-Ring

Base Seal

Washer

Retaining Nut Bottom Fittings

Terminal Fitting for Capillary Column

The SSL injector periodic maintenance includes:

• Replacing the SSL septum
Replace the septum at least after every 200 injections, or every time a problem related to
septum damage, or wear occurs.
See “Replacing the SSL Septum” on page 83.
• Cleaning or replacing the SSL liner
Check the liner for contaminants, debris, breakage, and proper installation.
The liner must be replaced depending on the number of injections performed, and the
characteristics of the samples injected. Typical symptoms will indicate that the liner must
be replaced. The most common is the appearance of tailing peaks in the chromatogram,
particularly for polar compounds.
See“Cleaning or Replacing the SSL Glass Liner” on page 84.

Tip It is good practice to replace the septum every time you replace the glass liner.

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

When replacing or removing a glass liner, it might break inside the injector. In this case
the broken parts of the liner must be removed from the injector, including the glass
splinters that might fall into the lower part of the vaporization chamber.
See “Replacing a SSL Broken Liner” on page 86.
• Replacing the active carbon filters on the carrier gas line and split line
The active carbon filters must be replaced depending on the volume of solvent injected in
the time.
See “Replacing the SSL Carrier and Split Lines Filters” on page 88.
• Replacing the body head o-rings
The internal (carrier line) and external (purge line) o-rings of the body head must be
replaced when in presence of leaks.
See “Replacing the SSL Body Head O-Rings” on page 89.

Before maintaining the injector, read the following warning:

WARNING The injector fittings could be hot. Carry out all the operation at low
temperature to avoid burns. Therefore, before beginning the sequence, the injector must
be cooled to room temperature.

CAUTION When handling organic solvents, you must take precautions to avoid health
hazards.

Materials needed to maintain a SSL injector

Septum
Tweezers
Glass liner
Liner seal (O-ring)
Ultrasonic cleaner
Mixture 1:1 methanol/acetone
Base Seal (if necessary)
Carrier gas line and/or split gas line active carbon filters
Body head internal o-ring
Body head external o-ring
T20 screwdriver

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

Note For maintaining or replacing any other component not listed in this section, see
Chapter 6, “Injectors Advanced Maintenance.”
• “Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF
Injectors” on page 230
• “Removing/Replacing an Injector Module” on page 231
• “Cleaning the SSL Injector Body” on page 234

Replacing the SSL Septum

 To replace the septum
Figure 77. SSL Injector: Septum Replacement

Septum Cap

Septum

Septum Holder

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the septum.
a. Unscrew and remove the septum cap.

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

b. Using tweezers, remove the septum from the septum holder.

c. Avoid touching the septum with your fingers. Insert a new septum into the septum
holder using tweezers.
d. Screw and tighten the septum cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

7. Close the module flap cover.

8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Cleaning or Replacing the SSL Glass Liner

 To clean or replace the glass liner
Figure 78. SSL Injector: Glass Liner Replacement

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal (O-ring)

Liner

1. Put the GC in standby condition.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

2. Cool the oven and injector to room temperature.

3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the liner.
a. Using tweezers, remove the liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it. Glass splinters might
fall into the lower part of the vaporization chamber. If the glass liner breaks, see “Replacing
a SSL Broken Liner” on page 86.

8. Replace or clean the liner.

• If you are going to use a new liner, go directly to step 10.
• If you are going to clean the dirty liner, go to step 9.
9. Clean the liner.
a. Put the liner into an ultrasonic cleaner filled with a methanol/acetone mixture (1:1).
b. Sonicate the liner for about half an hour.
c. Using tweezers, remove the liner from the bath and dry it with compressed clean air.
10. Install the liner.
a. Holding the new (or cleaned) liner with tweezers place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
11. Reinstall the top parts of the injector.
a. Place the septum holder/ liner cap with the septum on the body head of the injector,
and fix it by screwing the ring nut.
b. Screw and tighten the septum cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

12. Close the module flap cover.

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing a SSL Broken Liner

 To replace a broken liner
Figure 79. SSL Injector: Broken Liner Replacement

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal

Liner

Base Seal
Washer
Bottom Fittings
Retaining Nut
Ferrule

Capillary Column Retaining Nut Terminal Fitting for Capillary Column

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the broken liner.
a. Using tweezers, remove the upper part of broken liner with the liner seal from the
injector.
8. Remove the bottom parts of the injector.
a. Inside the oven, unscrew the capillary column retaining nut, then remove the
analytical column with its ferrule from the bottom of the injector.
b. From the bottom of the injector, unscrew the retaining nut with the washer and the
base seal. Glass splinters from the broken liner will fall from the injector.
c. With the aid of a pipe cleaner, remove the possible glass fragments from the
vaporization chamber.
9. Reinstall the bottom parts of the injector.
a. Reinstall the analytical column.
b. Reinstall the retaining nut with the new washer and the base seal. If necessary replace
the base seal with a new one.
10. Install a new liner.
a. Holding the new liner with tweezers, place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
11. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector
and fix them by screwing the ring nut.
b. Screw and tighten the injector cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

12. Close the module flap cover.

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector and GC working conditions.

Replacing the SSL Carrier and Split Lines Filters

IMPORTANT The dimensions of the filters are different. The filter on the split gas line is
bigger than the filter on the carrier gas line. Do not invert their position when you replace
them. It is not necessary to replace the filters together.

 To replace the active carbon filters on carrier gas line and split line

Figure 80. SSL Injector: Active Filters Replacement

Split Gas Line Active Carbon Filter

Carrier Gas Line Active Carbon Filter

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.

6. Replace the filter.

a. Remove the filter to replace from its seat by turning it counter-clockwise.
b. Install the new filter, with o-rings, in its seat by turning it clockwise.
7. Close the module flap cover.
8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Replacing the SSL Body Head O-Rings

 To replace the body head o-rings

Figure 81. SSL Injector: Body Head O-Rings Replacement

Body Head Internal O-ring (Carrier gas Line)

Body Head External O-ring (Purge gas Line)

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector (SSL)

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Replace the head body o-rings.
a. Use tweezer to remove the body head internal and external o-rings, and replace them
with new o-rings.
8. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector
and fix it by screwing the ring nut.
b. Screw and tighten the injector cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

9. Close the module flap cover.

10. If present, move the autosampler towards the module to restore the original alignment.
11. Turn the carrier gas on.
12. Set the normal injector, detector, and GC working conditions.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

This section provides instructions for maintaining a Split/Splitless injector for Backflush
(SSLBKF) applications.

The module and injector components are shown in Figure 82 and Figure 83.

Figure 82. SSLBKF Module Components

Module Flap Cover

Split Line Vent

Purge Line Vent

Septum Cap
Septum Holder/Liner Cap
Ring Nut

Carrier Gas Line Filter

Injector Body
Split Gas Line Filter

Module Cover

Insulation Cover

Bottom Fittings
Backflush Line

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

Figure 83. SSLBKF Injector Components

Septum Cap

Septum

Ring Nut

Septum Holder/Liner Cap

Liner Seal
Liner
Body Head External O-Ring

Body Head Internal O-Ring

Base Seal
Washer
Retaining Nut

(Terminal Fitting for Capillary Column) Backflush Line

Backflush Line Fitting

Ferrule
Fitting Cap, Backflush Line

The SSLBKF injector periodic maintenance includes:

• Replacing the SSLBKF septum
Replace the septum at least after every 200 injections, or every time a problem related to
septum damage, or wear occurs.
See “Replacing the SSLBKF Septum” on page 94.
• Cleaning or replying the SSLBKF liner
Check the liner for contaminants, debris, breakage, and proper installation. The liner
must be replaced depending on the number of injections performed, and the
characteristics of the samples injected. Typical symptoms will indicate that the liner must
be replaced. The most common is the appearance of tailing peaks in the chromatogram,
particularly for polar compounds.
See“Cleaning or Replacing the SSLBKF Glass Liner” on page 95.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

Tip It is good practice to replace the septum every time you replace the glass liner.

When replacing or removing a glass liner, it might break inside the injector. In this case
the broken parts of the liner must be removed from the injector, including the glass
splinters that might fall into the lower part of the vaporization chamber.
See “Replacing a SSLBKF Broken Liner” on page 97.
• Replacing the active carbon filters on the carrier gas line and split line
The active carbon filters must be replaced depending on the volume of solvent injected in
the time.
See “Replacing the SSLBKF Carrier and Split Lines Filters” on page 99.
• Replacing the body head o-rings
The internal (carrier line) and external (purge line) o-rings of the body head must be
replaced when in presence of leaks.
See “Replacing the SSLBKF Body Head O-Rings” on page 100.
Before maintaining the injector, read the following warning:

WARNING The injector fittings could be hot. Carry out all the operations at low
temperature to avoid burns. Therefore, before beginning the sequence, the injector must
be cooled to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed to maintain a SSLBKF injector

Septum
Tweezers
Glass liner
Liner seal (O-ring)
Ultrasonic cleaner
Mixture 1:1 methanol/acetone
Base Seal (if necessary)
Carrier gas line and/or split gas line active carbon filters
Body head internal o-ring
Body head external o-ring
T20 screwdriver

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 93

3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

Note For maintaining or replacing any other component not listed in this section, see
Chapter 6, “Injectors Advanced Maintenance.”
• “Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF
Injectors” on page 230
• “Removing/Replacing an Injector Module” on page 231
• “Cleaning the SSLBKF Injector Body” on page 238

Replacing the SSLBKF Septum

 To replace the septum

Figure 84. SSLBKF Injector: Septum Replacement

Septum Cap

Septum

Septum Holder

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the septum.
a. Unscrew and remove the septum cap.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

b. Using tweezers, remove the septum from the septum holder.

c. Avoid touching the septum with your fingers. Insert a new septum into the septum
holder using tweezers.
d. Screw and tighten the septum cap to finger-tight.
7. Close the module flap cover.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Cleaning or Replacing the SSLBKF Glass Liner

 To clean or replace the glass liner
Figure 85. SSLBKF Injector: Glass Liner Replacement

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal (O-ring)

Liner

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the liner.
a. Use tweezers to remove the liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it. Glass splinters might
fall into the lower part of the vaporization chamber. If the glass liner breaks, see “Replacing
a SSL Broken Liner” on page 86.

8. Replace or clean the liner.

• If you are going to use a new liner, go directly to step 10.
• If you are going to clean the dirty liner, go to step step 9.
9. Clean the liner.
a. Put the liner into an ultrasonic cleaner filled with a methanol/acetone mixture (1:1).
b. Sonicate the liner for about half an hour.
c. Using tweezers, remove the liner from the bath, and dry it with compressed clean air.
10. Install the liner.
a. Holding the new (or cleaned) liner with tweezers place a new liner seal over the liner.
b. Insert the liner into the injector and push it gently towards the bottom of the injector.
11. Reinstall the top parts of the injector.
a. Place the septum holder/ liner cap with the septum on the body head of the injector
and fix it by screwing the ring nut.
b. Screw and tighten the septum cap to finger-tight.

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

12. Close the module flap cover.

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing a SSLBKF Broken Liner

 To replace a broken liner
Figure 86. SSLBKF Injector: Broken Liner Replacement

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal

Liner

Base Seal
Washer

Retaining Nut
Bottom Fittings
Ferrule

Capillary Column Retaining Nut

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the broken liner.
a. Use tweezers to remove the upper part of broken liner with the liner seal from the
injector.
8. Remove the bottom parts of the injector.
a. Inside the oven, unscrew the capillary column retaining nut, then remove the
analytical column with its ferrule from the bottom of the injector.
b. From the bottom of the injector, unscrew the retaining nut with the washer and the
base seal. Glass splinters from the broken liner will fall from the injector.
c. With the aid of a pipe cleaner, remove the possible glass fragments from the
vaporization chamber.
9. Reinstall the bottom parts of the injector.
a. Reinstall the analytical column.
b. Reinstall the retaining nut with the new washer and the base seal (if necessary replace
the base seal with a new one).
10. Install a new liner.
a. Holding the new liner with tweezers, place a new liner seal over the liner.
b. Insert the liner into the injector and push it gently towards the bottom of the injector.
11. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector
and fix them by screwing the ring nut.
b. Screw and tighten the injector cap to finger-tight

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

12. Close the module flap cover.

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing the SSLBKF Carrier and Split Lines Filters

IMPORTANT The dimensions of the filters are different. The filter on the split gas line is
bigger than the filter on the carrier gas line. Do not invert their position when you replace
them. It is not necessary to replace the filters together.

 To replace the active carbon filters on carrier gas line and split line

Figure 87. SSLBKF Injector: Active Filters Replacement

Split Gas Line Active Carbon Filter

Carrier Gas Line Active Carbon Filter

1. Put the GC in standby condition.

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3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

2. Cool the oven and injector to room temperature.

3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.

6. Replace the filter.

a. Remove the filter to replace from its seat by turning it counter-clockwise.
b. Install the new filter, with o-rings, in its seat by turning it clockwise.
7. Close the module flap cover.
8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Replacing the SSLBKF Body Head O-Rings

 To replace the body head o-rings

Figure 88. SSLBKF Injector: Body Head O-Rings Replacement

Body Head Internal O-ring (Carrier gas Line)

Body Head External O-ring (Purge gas Line)

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 3 Performing Injectors Routine Maintenance
Maintaining a Split/Splitless Injector with Backflush (SSLBKF)

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Replace the head body o-rings.
a. Use tweezers to remove the body head internal and external o-rings, and replace them
with new o-rings.
8. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector
and fix it by screwing the ring nut.
b. Screw and tighten the injector cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

9. Close the module flap cover.

10. If present, move the autosampler towards the module to restore the original alignment.
11. Turn the carrier gas on.
12. Set the normal injector, detector, and GC working conditions.

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3 Performing Injectors Routine Maintenance
Maintaining a Gas Sampling Valve Injector (GSV)

Maintaining a Gas Sampling Valve Injector (GSV)

This section provides instructions for maintaining a Gas Sampling Valve injector (GSV)
module.

The module and injector components are shown in Figure 89.

Figure 89. GSV Module Components

Sample IN
Sample OUT (WASTE)
Split Line Active Carbon

Split Line Vent

Carrier Line Active Carbon

6-ports Diaphragm Valve

Purge Tubing

Sample Loop

Relief Pins

Bottom Fittings
Backflush Line

IMPORTANT The valve is delivered with relief pins. These pins MUST BE removed from
the valve body before working with the valve.

Once actuation tubing has been set up, and pressure

has been adjusted to 450 kPa (65 psig), actuate the
valve and remove the pins. You may need to increase
the pressure a little to ease hand removal of the pins.

Remember, make sure to properly readjust the

actuating operating pressure after removing the pins,
Relief if you had to change it. Keeps these pins in a safe
Pins place. You may want to re-use them for valve
maintenance. It is good practice to re-install the relief
pins if the valve is not used for a long time.
For details refer to the instructions provided by the valve manufacturer.

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 3 Performing Injectors Routine Maintenance
Maintaining a Gas Sampling Valve Injector (GSV)

The Gas Sampling Valve periodic maintenance includes:

• Connecting the sample In and Out lines
See Connecting the Sample In and Out Lines.
• Replacing the active carbon filters on the carrier gas line and split line
The active carbon filters must be replaced depending on the volume of solvent injected in
the time.
See Replacing the Carrier and Split Lines Filters.
•Replacing the sample loop
Replace the sample loop when an upper volume of sample than the volume of standard
loop is required. Choose among the loops available.
See Replacing the Sample Loop.
•Replacing the gas sampling valve diaphragm
Follow the instructions provided by the manufacturer of the valve.

Connecting the Sample In and Out Lines

 To connect the sample In and Out lines

1. If not already done, remove the protective caps from the Sample In and Sample Out
fittings. See Figure 90.

Figure 90. Protective Caps

1. By using the proper 1/8-in. tubing, nut and ferrule, connect the inlet sample line to the
Sample In port on the GSV module. See Figure 91.

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3 Performing Injectors Routine Maintenance
Maintaining a Gas Sampling Valve Injector (GSV)

Figure 91. GSV Sample In Line Connection

IN

2. By using the proper 1/8-in. tubing, nut and ferrule, connect the Sample OUT port on
the GSV module to an exhaust system. See Figure 92.

Figure 92. GSV Sample Out Line Connection

OUT

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 3 Performing Injectors Routine Maintenance
Maintaining a Gas Sampling Valve Injector (GSV)

Figure 93. GSV Sample In and Out Lines Connection

Sample OUT /Waste

Sample IN

Replacing the Carrier and Split Lines Filters

IMPORTANT The dimensions of the filters are different. The filter on the split gas line is
bigger than the filter on the carrier gas line. Do not invert their position when you replace
them. It is not necessary to replace the filters together.

 To replace the active carbon filters on carrier gas line and split line

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the filter.
a. Remove the filter to replace from its seat by turning it counter-clockwise.
b. Install the new filter, with o-rings, in its seat by turning it clockwise.

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3 Performing Injectors Routine Maintenance
Maintaining a Gas Sampling Valve Injector (GSV)

Figure 94. GSV Module Active Filters Replacement

Split Gas Line Active Carbon Filter
Carrier Gas Line Active Carbon Filter

Filter O-rings

7. Close the module flap cover.

8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Replacing the Sample Loop

 To replace the sampling loop

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the sampling loop.
a. Use a 3/16-in.wrench to loosen the two nuts connecting the sample loop to the
six–port valve.
b. Remove the loop off the valve.
c. Mount the new loop over the valve and tighten the nuts to the six-port valve.

106 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Gas Sampling Valve Injector (GSV)

Figure 95. Example of Sample Loop Replacement

Sample Loop

7. Close the module flap cover.

8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 107
3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

This section provides instructions for maintaining an Instant Connect Helium Saver Injector
Module (HeS-S/SL).

The module and injector components are shown in Figure 96 and Figure 97.

Figure 96. HeS-S/SL Module Components

Split Line Vent
Purge Line Vent
Septum Cap
Septum Holder/Liner Cap
Ring Nut
Carrier Gas Line Filter

Split Gas Line Filter

Helium Transfer Tube

Insulation Cover
Bottom Fittings

108 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

Figure 97. HeS-S/SL Module Components

Septum Cap

Septum
Ring Nut

Septum Holder/Liner Cap

Liner Seal

Liner

Body Head External O-Ring

Body Head Internal O-Ring
Bottom Fittings

Maintaining the Instant Connect Helium Saver Injector Module is largely the same as a
conventional SSL inlet.
One advantage of the Instant Connect Helium Saver Injector Module is that routine septum
and liner changes can be accomplished without cooling the MS transfer line or ion source.
This is particularly desirable since cooling down and re-establishing stable MS temperatures
takes much longer than cooling down and re-establishing the inlet temperature.

The Instant Connect Helium Saver Injector Module periodic maintenance includes:
• Replacing the septum
The septum needs to be changed intermittently to prevent leakage. Replace the septum at
least after every 200 injections, or every time a problem related to septum damage, or
wear occurs.
See “Replacing the Septum” on page 111.
• Cleaning or replacing the liner
Injection port liner needs to be replaced or cleaned as it becomes dirty.
See“Cleaning or Replacing the Glass Liner” on page 112.

Tip It is good practice to replace the septum every time you replace the glass liner.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 109
3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

• Replacing the active carbon filters on the carrier gas line and split line
The active carbon filters must be replaced depending on the volume of solvent injected in
the time.
See “Replacing the Carrier and Split Lines Filters” on page 114.
• Replacing the body head o-rings
The internal (carrier line) and external (purge line) o-rings of the body head must be
replaced when there are leaks present.
See “Replacing the Body Head O-Rings” on page 115.

Before maintaining the injector, read the following warning:

WARNING The injector fittings could be hot. Carry out all the operation at low
temperature to avoid burns. Therefore, before beginning the sequence, the injector must
be cooled to room temperature.

CAUTION When handling organic solvents, you must take precautions to avoid health
hazards.

Materials needed to maintain a HeS-S/SL

Septum
Tweezers
Glass liner
Liner seal (O-ring)
Ultrasonic cleaner
Mixture 1:1 methanol/acetone
Carrier gas line and/or split gas line active carbon filters
Body head internal o-ring
Body head external o-ring
T20 screwdriver

Note For maintaining or replacing any other component not listed in this section, see
Chapter 6, “Injectors Advanced Maintenance.”
• “Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF
Injectors” on page 230
• “Cleaning the HeS-S/SL Injector Body” on page 242

110 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

Replacing the Septum

 To replace the septum
Figure 98. HeS-S/SL Injector: Septum Replacement

Septum Cap

Septum

Septum Holder

Note Care should be taken when performing this procedure in order to keep from
damaging analytical columns.

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Ensure the Enable conservation field in the configuration page of the inlet reads Yes.
4. Check that there is at least 20 mL/min split flow exiting the split line, then turn the
column flow to Off in the GC user interface and allow the inlet to depressurize.
5. Put the autosampler away if present.
6. Open the module flap cover.
7. Replace the septum.
a. Unscrew and remove the septum cap.
b. Using tweezers, remove the septum from the septum holder.
c. Avoid touching the septum with your fingers. Insert a new septum into the septum
holder using tweezers.
d. Screw and tighten the septum cap to finger-tight.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 111
3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

8. Close the module flap cover.

9. If present, move the autosampler towards the module to restore the original alignment.
10. Wait two or three minutes for helium to purge the inlet.
11. Turn the inlet flow back on and enable the inlet heater.
12. Set the normal injector, detector, and GC working conditions.

Cleaning or Replacing the Glass Liner

 To clean or replace the glass liner
Figure 99. HeS-S/SL Injector: Glass Liner Replacement

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal (O-ring)

Liner

Note Care should be taken when performing the procedure in order to keep from
damaging analytical columns.

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

112 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

3. Ensure the Enable conservation field in the configuration page of the inlet reads Yes.
4. Check that there is at least 20 mL/min split flow exiting the split line, then turn the
column flow to Off in the GC user interface and allow the inlet to depressurize.
5. Put the autosampler away if present.
6. Open the module flap cover.
7. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
8. Remove the liner.
a. Using tweezers, remove the liner with the liner seal (o-ring) from the injector.
9. Replace or clean the liner.
• If you are going to clean the dirty liner, go to step 10.
• If you are going to use a new liner, go directly to step 11.
10. Clean the liner.
a. Put the liner into an ultrasonic cleaner filled with a methanol/acetone mixture (1:1).
b. Sonicate the liner for about half an hour.
c. Using tweezers, remove the liner from the bath and dry it with compressed clean air.
11. Install the liner.
a. Hold the new (or cleaned) liner with tweezers place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
12. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector,
and fix it by screwing the ring nut.
b. Screw and tighten the septum cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

13. Close the module flap cover.

14. If present, move the autosampler towards the module to restore the original alignment.
15. Wait two or three minutes for helium to purge the inlet.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 113
3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

16. Turn the inlet flow back on and enable the inlet heater.
17. Set the normal injector, detector, and GC working conditions.

Replacing the Carrier and Split Lines Filters

CAUTION The dimensions of the filters are different. The filter on the split gas line is
bigger than the filter on the carrier gas line. Do not invert their position when you replace
them. It is not necessary to replace the filters together.

 To replace the active carbon filters on carrier gas line and split line

Figure 100. HeS-S/SL Injector: Active Filters Replacement

Split Gas Line Active Carbon Filter
Carrier Gas Line Active Carbon Filter

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Ensure the Enable conservation field in the configuration page of the inlet reads Yes.
4. Check that there is at least 20 mL/min split flow exiting the split line, then turn the
column flow to Off in the GC user interface and allow the inlet to depressurize.

114 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

5. Put the autosampler away if present.

6. Open the module flap cover.

7. Replace the filter.

a. Remove the filter to replace from its seat by turning it counter-clockwise.
b. Install the new filter, with o-rings, in its seat by turning it clockwise.
8. Close the module flap cover.
9. If present, move the autosampler towards the module to restore the original alignment.
10. Wait two or three minutes for helium to purge the inlet.
11. Turn the inlet flow back on and enable the inlet heater.
12. Set the normal injector, detector, and GC working conditions.

Replacing the Body Head O-Rings

 To replace the body head o-rings

Figure 101. HeS-S/SL Injector: Body Head O-Rings Replacement

Body Head Internal O-ring (Carrier gas Line)

Body Head External O-ring (Purge gas Line)

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 115
3 Performing Injectors Routine Maintenance
Maintaining an Instant Connect Helium Saver Injector (HeS-S/SL)

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Ensure the Enable conservation field in the configuration page of the inlet reads Yes.
4. Check that there is at least 20 mL/min split flow exiting the split line, then turn the
column flow to Off in the GC user interface and allow the inlet to depressurize.
5. Put the autosampler away if present.
6. Open the module flap cover.
7. Remove the top parts of the injector.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
8. Replace the head body o-rings.
a. Use tweezers to remove the body head internal and external o-rings, and replace them
with new o-rings.
9. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector
and fix it by screwing the ring nut.
b. Screw and tighten the injector cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

10. Close the module flap cover.

11. If present, move the autosampler towards the module to restore the original alignment.
12. Wait two or three minutes for helium to purge the inlet.
13. Turn the inlet flow back on and enable the inlet heater.
14. Set the normal injector, detector, and GC working conditions.

116 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

Maintaining a Programmable Temperature Vaporizing Injector (PTV)

This section provides instructions for maintaining a Programmable Temperature Vaporizing
injector (PTV).

The module and injector components are shown in Figure 102 and Figure 103.

Figure 102. PTV Module Components

Module Flap Cover

Split Line Vent

Purge Line Vent
Carrier Line Filter
Septum Cap
Injector Head Assembly
Injector Body

Cooling Fan

Split Line Filter

Module Cover

Insulator Cover

Bottom Fittings

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 117
3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

Figure 103. PTV Injector Components

Septum Cap

Septum

Liner Cap

Liner Seal

Liner

Silver Seal

Terminal Fitting for Capillary Column

Ferrule
Split Nut

The PTV injector periodic maintenance includes:

• Replacing the PTV septum
Replace the septum at least after every 200 injections, or every time a problem related to
septum damage, or wear occurs.
See “Replacing the PTV Septum” on page 120.
• Cleaning or replacing the PTV liner
Check the liner for contaminants, debris, breakage and proper installation.

118 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

The liner must be replaced depending on the number of injections performed, and the
characteristics of the samples injected. Typical symptoms will indicate that the liner must
be replaced. The most common is the appearance of tailing peaks in the chromatogram,
particularly for polar compounds.
Tip It is good practice to replace the septum every time you replace the gas liner.

See “Cleaning or Replacing the PTV Glass Liner” on page 121.

When replacing or removing a glass liner, it might break inside the injector. In this case
the broken parts of the liner must be removed from the injector, including the glass
splinters that might fall into the lower part of the vaporization chamber.
See “Replacing the PTV Broken Liner” on page 123.
• Replacing the active carbon filters on the carrier gas line and split line
The active carbon filters must be replaced depending on the volume of solvent injected in
the time.
See “Replacing the PTV Carrier and Split Lines Filters” on page 125.

Before maintaining the injector, read the following warning:

WARNING The injector fittings could be hot. Carry out all the operation at low
temperature to avoid burns. Therefore, before beginning the sequence, the injector must
be cooled to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazard.

Materials needed to maintain a PTV injector

Septum
Tweezers
Glass liner
Liner seal (O-ring)
Ultrasonic cleaner
Mixture 1:1 methanol/acetone
Carrier gas line and/or split gas line active carbon filters
5.5 x 25 mm slotted stubby driver
Silver seal

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 119
3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

Note For maintaining or replacing any other component not listed in this section, see
Chapter 6, “Injectors Advanced Maintenance.”
• “Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF
Injectors” on page 230
• “Removing/Replacing an Injector Module” on page 231
• “Cleaning the PTV Injector Head Assembly” on page 246

Replacing the PTV Septum

 To replace the septum

Figure 104. PTV Injector: Septum Replacement

Septum Cap

Septum

Injector Head Assembly

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the septum.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the injector head assembly.

120 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

c. Avoid touching the septum with your fingers. Insert a new septum into the injector
head assembly using tweezers. Screw and tighten the septum cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

7. Close the module flap cover.

8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Cleaning or Replacing the PTV Glass Liner

 To clean or replace the glass liner

Figure 105. PTV Injector: Glass Liner Replacement

Septum Cap

Septum

Liner Cap

Liner Seal (O-ring)

Liner

Injector Head Assembly

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 121
3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top part of the injector.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the top of the injector head assembly.
7. Remove the liner.
a. Using the slotted stubby driver provided, unscrew and remove the liner cap.
b. Use tweezers to remove the liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it. Glass splinters might
fall into the lower part of the vaporization chamber. If the glass liner breaks, see “To
replace a broken liner” on page 86.

8. Replace or clean the liner.

• If you are going to use a new liner, go directly to step 10.
• If you are going to clean the dirty liner, go to step 9.
9. Clean the liner.
a. Put the liner into an ultrasonic cleaner filled with a methanol/acetone mixture (1:1).
b. Sonicate the liner for about half an hour.
c. Using tweezers, remove the liner from the bath, and dry it with compressed clean air.
10. Install the liner
a. Holding the new (or cleaned) liner with tweezers place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
c. Reinsert and screw the liner cap using the slotted stubby driver.
11. Reinstall the top parts of the injector.
a. Place the septum into the injector head assembly.

CAUTION It is suggested to replace the septum with a new one.

b. Screw and tighten the septum cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

12. Close the module flap cover.

122 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing the PTV Broken Liner

 To replace a broken liner

Figure 106. PTV Injector: Broken Liner Replacement

Septum Cap

Septum

Liner Cap
Liner Seal

Liner

Silver Seal
Bottom Fittings
Terminal Fitting

Ferrule
Split Nut

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 123
3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top part of the injector.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the top of the injector head assembly.
7. Remove the broken liner.
a. Using the slotted stubby driver provided, unscrew and remove the liner cap.
b. Use tweezers to remove the upper part of broken liner with the liner seal from the
injector.
8. Remove the bottom parts of the injector.
a. Inside the oven, unscrew the split nut, and remove the analytical column with its
ferrule from the terminal fitting at the bottom of the injector.
b. Unscrew the terminal fitting with the silver seal. Glass splinters from the broken liner
will fall from the injector.
c. With the aid of a pipe cleaner, remove the possible glass fragments from the
vaporization chamber.
9. Reinstall the bottom part of the injector.
a. Reinstall the terminal fitting with the silver seal.
b. Reinstall the analytical column.
10. Install a new liner.
a. Holding the new liner with tweezers, place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
c. Reinsert and screw the liner cap using the slotted stubby driver.
11. Reinstall the top parts of the injector.
a. Place the septum into the injector head assembly.

CAUTION It is suggested to replace the septum with a new one.

b. Screw and tighten the injector cap to finger-tight.

124 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector (PTV)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

12. Close the module flap cover.

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing the PTV Carrier and Split Lines Filters

IMPORTANT The dimensions of the filters are different. The filter on the split gas line is
bigger than the filter on the carrier gas line. Do not invert their position when you replace
them. It is not necessary to replace the filters together.

 To replace the active carbon filters on carrier gas line and split line

Figure 107. PTV Injector: Active Filters Replacement

Carrier Gas Line Filter

Split Gas Line Filter

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 125
 1. Put the GC in standby condition.
2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the filter.
a. Remove the filter to replace from its seat by turning it counter-clockwise.
b. Install the new filter, with the o-rings, in its seat by turning it clockwise.
7. Close the module flap cover.
8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

Maintaining a Programmable Temperature Vaporizing Injector with

Backflush (PTVBKF)
This section provides instructions for maintaining a Programmable Temperature Vaporizing
injector for backflush (PTVBKF) applications.

The module and injector components are shown in Figure 108 and Figure 109.

Figure 108. PTVBKF Module Components

Module Flap Cover

Split Line Vent

Purge Line Vent
Carrier Line Filter
Septum Cap
Injector Head Assembly
Injector Body

Cooling Fan

Split Line Filter

Module Cover

Insulator Cover

Bottom Fittings Backflush Line

Backflush Line Fitting

Fitting Cap, Backflush Line

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 127
3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

Figure 109. PTVBKF Injector Components

Septum Cap

Septum

Liner Cap

Liner Seal
Liner

Silver Seal

Terminal Fitting for Capillary Column

Ferrule
Split Nut
Backflush Line

Backflush Line Fitting

Ferrule
Fitting Cap, Backflush Line

The PTVBKF injector periodic maintenance includes:

• Replacing the PTVBKF septum
Replace the septum at least after every 200 injections, or every time a problem related to
septum damage, or wear occurs.
See “Replacing the PTVBKF Septum” on page 130.

128 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

• Cleaning or replacing the PTVBKF liner

Check the liner for contaminants, debris, breakage and proper installation. The liner
must be replaced depending on the number of injections performed, and the
characteristics of the samples injected. Typical symptoms will indicate that the liner must
be replaced. The most common is the appearance of tailing peaks in the chromatogram,
particularly for polar compounds.
See “Cleaning or Replacing the PTVBKF Glass Liner” on page 131
Tip It is good practice to replace the septum every time the liner is replaced.

When replacing or removing a glass liner, it might break inside the injector. In this case
the broken parts of the liner must be removed from the injector, including the glass
splinters that might fall into the lower part of the vaporization chamber.
See “Replacing the PTVBKF Broken Liner” on page 133.
• Replacing the PTVBKF active carbon filters on the carrier gas line and split line
The active carbon filters must be replaced depending on the volume of solvent injected in
the time.
See Replacing the PTVBKF Carrier and Split Lines Filters on page 135.

Before maintaining the injector, read the following warning:

WARNING The injector fittings could be hot. Carry out all operations at low temperature
to avoid burns. Therefore, before beginning the sequence, the injector must be cooled to
room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed to maintain a PTVBKF injector

Septum
Tweezers
Glass liner
Liner seal (O-ring)
Ultrasonic cleaner
Mixture 1:1 methanol/acetone
.
Carrier gas line and/or split gas line active carbon filters
5.5 x25 mm slotted stubby driver
Silver seal

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 129
3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

Note For maintaining or replacing any other component not listed in this section, see
Chapter 6, “Injectors Advanced Maintenance.”
• “Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF
Injectors” on page 230
• “Removing/Replacing an Injector Module” on page 231
• “Cleaning the PTVBKF Injector Head Assembly” on page 253

Replacing the PTVBKF Septum

 To replace the septum

Figure 110. PTVBKF Injector: Septum Replacement

Septum Cap

Septum

Injector Head Assembly

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Replace the septum.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the injector head assembly.
c. Avoid touching the septum with your fingers. Insert a new septum into the injector
head assembly using tweezers. Screw and tighten the septum cap to finger-tight.

130 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

7. Close the module flap cover.

8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

Cleaning or Replacing the PTVBKF Glass Liner

 To clean or replace the glass liner

Figure 111. PTVBKF Injector: Glass Liner Replacement

Septum Cap

Septum

Liner Cap

Liner Seal (O-ring)

Liner

Injector Head Assembly

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

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3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top part of the injector.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the top of the injector head assembly.
7. Remove the liner.
a. Using the slotted stubby driver provided, unscrew and remove the liner cap.
b. Use tweezers to remove the liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it. Glass splinters might
fall into the lower part of the vaporization chamber. If the glass liner breaks, see
“Replacing the PTVBKF Broken Liner” on page 133.

8. Replace or clean the liner.

• If you are going to use a new liner, go directly to step 9.
• If you are going to clean the dirty liner, go to step 10.
9. Clean the liner.
a. Put the liner into an ultrasonic cleaner filled with a methanol/acetone mixture (1:1).
b. Sonicate the liner for about half an hour.
c. Using tweezers, remove the liner from the bath, and dry it with compressed clean air.
10. Install the liner.
a. Holding the new (or cleaned) liner with tweezers, place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
c. Reinsert and screw the liner cap using the slotted stubby driver.
11. Reinstall the top parts of the injector.
a. Place the septum into the injector head assembly.

CAUTION It is suggested to replace the septum with a new one.

b. Screw and tighten the septum cap to finger-tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

12. Close the module flap cover.

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 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing the PTVBKF Broken Liner

 To replace a broken liner

Figure 112. PTVBKF Injector: Broken Liner Replacement

Septum Cap

Septum

Liner Cap

Liner Seal

Liner

Silver Seal
Bottom Fittings
Terminal Fitting

Ferrule
Backflush Line
Split Nut

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

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3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top part of the injector.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the top of the injector head assembly.
7. Remove the broken liner.
a. Using the slotted stubby driver provided, unscrew and remove the liner cap.
b. Use tweezers to remove the upper part of broken liner with the liner seal from the
injector.
8. Remove the bottom parts of the injector.
a. Inside the oven, unscrew the split nut, and remove the analytical column with its
ferrule from the terminal fitting at the bottom of the injector.
b. Unscrew the terminal fitting with the silver seal. Glass splinters from the broken liner
will fall from the injector.
c. With the aid of a pipe cleaner, remove the possible glass fragments from the
vaporization chamber.
9. Reinstall the bottom part of the injector.
a. Reinstall the terminal fitting with the silver seal (if necessary replace it with a new
one).
b. Reinstall the analytical column.
10. Install a new liner.
a. Holding the new liner with tweezers, place a new liner seal over the liner.
b. Insert the liner into the injector, and push it gently towards the bottom of the
injector.
c. Reinsert and screw the liner cap using the slotted stubby driver.
11. Reinstall the top parts of the injector.
a. Place the septum into the injector head assembly.

CAUTION It is suggested to replace the septum with a new one.

b. Screw and tighten the injector cap to finger-tight.

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 3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

12. Close the module flap cover.

13. If present, move the autosampler towards the module to restore the original alignment.
14. Turn the carrier gas on.
15. Set the normal injector, detector, and GC working conditions.

Replacing the PTVBKF Carrier and Split Lines Filters

IMPORTANT The dimensions of the filters are different. The filter on the split gas line is
bigger than the filter on the carrier gas line. Do not invert their position when you replace
them. It is not necessary to replace the filters together.

 To replace the active carbon filters on carrier gas line and split line

Figure 113. PTVBKF Injector: Active Filters Replacement

Carrier Gas Line Filter

Split Gas Line Filter

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3 Performing Injectors Routine Maintenance
Maintaining a Programmable Temperature Vaporizing Injector with Backflush (PTVBKF)

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.

6. Replace the filter.

a. Remove the filter to replace from its seat by turning it counter-clockwise.
b. Install the new filter, with the o-rings, in its seat by turning it clockwise.
7. Close the module flap cover.
8. If present, move the autosampler towards the module to restore the original alignment.
9. Turn the carrier gas on.
10. Set the normal injector, detector, and GC working conditions.

136 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 4

Performing Detectors Routine Maintenance

This chapter provides instructions for performing routine maintenance on the TRACE 1300
and TRACE 1310 detectors modules.

Contents
• Maintaining a Flame Ionization Detector (FID)
• Maintaining a Nitrogen Phosphorous Detector (NPD)
• Maintaining a Thermal Conductivity Detector (TCD)
• Maintaining an Electron Capture Detector (ECD)
• Maintaining a Flame Photometric Detector (FPD)
• Maintaining a Pulsed Discharge Detector (PDD)

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

Maintaining a Flame Ionization Detector (FID)

This section provides instructions for maintaining a Flame Ionization Detector (FID).
The module and detector components are shown in Figure 114 and Figure 115.

Figure 114. FID: Module Components

Vent

Polarizing Electrode

Module Flap Cover

Signal Cable
Glow-plug Cable

Glow-plug
Detector Cell Assembly

Detector Body
Polarizing Cable

Insulation Cover

Bottom Fittings

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

Figure 115. FID: Cell Assembly Components

Detector Cap

Cell Top Cover

Insulator Ring

Collecting Electrode

Collector Insulator
Glow-plug Cable
Signal Cable
Detector Body (see
Polarizing Electrode
Polarizing Cable

Figure 116. FID: Jet and Polarizing Electrode

Jet

Polarizing Electrode

To keep optimum performance of the FID, you must keep it clean and free of dust and
deposits. Symptoms, such as reduced sensitivity and increased noise, indicate that the detector
needs cleaning.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

The FID detector periodic maintenance includes:

• Cleaning the collecting electrode
It is suggest to clean the collecting electrode annually, or when a high noise baseline is
found, due to some compounds that produce detector contamination. If necessary,
replace the collecting electrode.
See “Cleaning or Replacing the FID Collecting Electrode” on page 141.
• Replacing the FID ignition glow-plug
This operation is NOT part on the ordinary maintenance. The flame ignition element
must be replaced only when defective.
See “Replacing the FID Ignition Glow-plug” on page 144.

Before maintaining the detector, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed to maintain a FID detector

Ultrasonic cleaner
Liquid detergent
GC-grade methanol
Distilled water
FID Ignition Glow-plug
Collecting electrode (if necessary)
8-mm elbowed box wrench
T6 Torxhead screwdriver
Forceps or tweezers

Note For maintaining or replacing any other component not listed in this section, see
Chapter 7, “Detectors Advanced Maintenance.”
• “Removing/Replacing a Detector Module” on page 262
• “Cleaning or Replacing the FID Jet” on page 271

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

Cleaning or Replacing the FID Collecting Electrode

 To clean or replace the collecting electrode

1. Put the GC in standby condition.

2. Turn the flame off. The fuel gases, hydrogen and air, are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
6. If external modules are present, unplug the power cable from the AC Input connector of
each module, and from the wall outlet.
7. Open the module flap cover.
8. Disconnect the signal, glow-plug, and polarizing cables from their contacts on the cell.

Figure 117. FID Cables

Signal Cable Straight Plug Crimp

Connector

Socket Set Screw

Glow-plug

Polarizing Cable Straight Jack Connector

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

Figure 118. FID Cables Disconnection

Collecting Electrode Bulkhead Jack

Glow-plug Dowel Pin

Polarizing Electrode Bulkhead Jack

a. Loosen the glow-plug cable socket set screw using a T6 Torxhead screwdriver.
Carefully pull out the terminal body of the glow-plug cable from the dowel pin on
the glow-plug element.
b. Unscrew and pull out the straight plug crimp connector of the signal cable from the
collecting electrode bulkhead jack.
c. Pull out the quick coupling straight jack connector of the polarizing cable from the
polarizing electrode bulkhead jack.
d. Carefully move the cables in order to have free space for handling the detector.
9. Remove the top parts of the detector.

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

Figure 119. FID Collecting Electrode

Detector Cap

Cell Top Cover

Insulator Ring

Collecting Electrode

a. Unscrew and remove the detector cap, paying attention to not rotate the cell top
cover.
b. Remove the cell top cover and put it on a safe place. Pay attention to not damaging
the FID collecting electrode pin.
10. Remove, clean and reinstall the collecting electrode.
a. Using forceps or tweezers, carefully extract the collecting electrode and the insulator
ring through the top of the detector cell.
b. Slip off the insulator ring from the collecting electrode.

WARNING The insulator ring must be placed carefully away in a place where it stays
clean.

c. Place the collecting electrode in the ultrasonic cleaner filled with liquid detergent.
d. Sonicate the collecting electrode for about five minutes.
e. Handling the collecting electrode with forceps or tweezers, rinse it using distilled
water, then methanol.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

f. Place the collecting electrode on a paper towel and let it air dry.
Note If after the cleaning the physical condition of the collecting electrode does
not permit its reuse, replace the electrode with a new one.

g. Place the cleaned (or a new) collecting electrode and the insulator ring in its housing.
11. Reinstall the top parts of the detector.
a. Remount the cell top cover, then screw the detector cap, paying attention to not
rotate the cell top cover.
b. Reconnect the signal, glow-plug, and polarizing cables.
12. Close the module flap cover.
13. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
14. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
15. Set the make-up gas on.
16. Set the normal detector working conditions.
17. Ignite the flame.

Replacing the FID Ignition Glow-plug

 To replace the ignition glow-plug

1. Put the GC in standby condition.

2. Turn the flame off. The fuel gases, hydrogen and air, are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

6. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
7. Open the module flap cover.
8. Disconnect the signal cable, glow-plug and polarizing cables from their contacts on the
cell top cover.

Figure 120. FID Cables

Signal Cable Straight Plug Crimp

Connector

Socket Set Screw

Glow-plug Cable Terminal body

Polarizing Cable Straight Jack Connector

Figure 121. FID Cables Disconnection

Collecting Electrode (Anode)

Bulkhead Jack

Glow-plug Dowel Pin

Polarizing Electrode Bulkhead Jack

a. Loosen the glow-plug cable socket set screw using a T6 Torxhead screwdriver.
Carefully pull out the terminal body of the glow-plug cable from the dowel pin on
the glow-plug element.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Ionization Detector (FID)

b. Unscrew and pull out the straight plug crimp connector of the signal cable from the
collecting electrode (anode) bulkhead jack.
c. Pull out the quick coupling straight jack connector of the polarizing cable from the
polarizing electrode bulkhead jack.
d. Carefully move the cables in order to have free space for handling the detector.
9. Replace the defective glow-plug.

Figure 122. FID Glow-plug Replacement

Glow-plug

a. Using an 8-mm wrench, unscrew and remove the defective glow-plug with its washer.
b. Replace the glow-plug with a new one interposing the new washer.
c. Reconnect the glow-plug, signal, and polarizing cables.
10. Close the module flap cover.
11. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Turn the make-up gas on.
14. Set the normal detector working conditions.
15. Ignite the flame.

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Maintaining a Nitrogen Phosphorous Detector (NPD)

This section provides instructions for maintaining a Nitrogen Phosphorous Detector (NPD).
The components of the module and detector are shown in Figure 123, Figure 124, and
Figure 125.

Figure 123. NPD Module Components

Signal Cable
Thermionic Source Assembly
Cable

Thermionic Source Assembly

Module Flap Cover

NPD Thermionic Source Power Module

Thermionic Source Assembly

Vent

Collecting Electrode Cable

Bottom Fittings

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Figure 124. NPD Cell Assembly Components

Thermionic Source
Assembly

Collecting Electrode

Insulator

Detector Body
See Figure 125

Collecting Electrode
Cable

Thermionic Source
Assembly Cable

Signal Cable

Figure 125. NPD Detector: Jet and Collecting Electrode (Anode) Pin

Jet
Collecting Electrode Pin

Collecting Electrode Straight Jack Connector

To ensure optimum performance of the NPD, you must keep it clean and free of dust and
deposits. Symptoms such as reduced sensitivity and increased noise indicate that detector
cleaning, or thermionic source replacement could be necessary.

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Note The thermionic source is supplied by a separated NPD Thermionic Source Power
Module placed into an external module housing on the back of the GC.

The NPD detector periodic maintenance includes:

• Replacing the thermionic source
New thermionic sources may require slightly different values of source heating current to
produce the same signal observed with a previous thermionic source.
See “Replacing the NPD Thermionic Source” on page 150.
• Cleaning the collecting electrode
We suggest cleaning the collecting electrode semiannually, or when a high noise baseline
is found, due to some compounds that produce detector contamination. If necessary,
replace the collecting electrode.
See “Cleaning or Replacing the NPD Collecting Electrode” on page 156.

Before maintaining the detector, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed to maintain a NPD detector

Thermionic Source
Gloves
Ultrasonic cleaner
Liquid detergent
GC-grade methanol
Distilled water
Collecting electrode (if necessary)
Forceps or tweezers
T6 Torxhead key
T10 Torxhead screwdriver
6 mm wrench

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Note For maintaining or replacing any other component not listed in this section, see
Chapter 7, “Detectors Advanced Maintenance.”
• “Measuring the NPD Gas Flows” on page 276
• “Cleaning or Replacing the NPD Jet” on page 285

Replacing the NPD Thermionic Source

CAUTION The thermionic source is delicate. Be careful not to break or crack the source.
When performing maintenance on the NPD, avoid touching the source with your fingers,
and prevent it from coming in contact with other surfaces. Wear clean, lint-free gloves to
prevent contamination of parts with dirt and skin oils.

 To replace the thermionic source

1. Put the GC in standby condition.

2. Switch off the thermionic source. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
6. Unplug the power cable from the AC input connector of the NPD Thermionic Source
Power Module and from the wall outlet.
If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
7. Unplug the signal cable from the detector module. See Figure 126.

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Figure 126. Unplug NPD Signal Cable

8. Open the module flap cover.

9. Remove the thermionic source assembly cable.
a. Twist the ring to disconnect the thermionic source assembly cable.
b. Push and twist the lock so that the button slides up in the groove, then pull the cable
ends apart. See Figure 127.
Figure 127. NPD Thermionic Source Cable Removal

Thermionic Source Assembly Cable

Thermionic Source Assembly

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

10. Unscrew and pull out the straight jack connector of the collecting electrode cable from
the collecting electrode bulkhead jack. See Figure 128.

Figure 128. Collecting Electrode Cable Removal

Collecting Electrode Cable

Collecting Electrode Straight Jack

Connector

11. Remove the thermionic source.

a. Using a T6 Torxhead key, loosen the dowel which fix the thermionic source
connector to the thermionic source support. See Figure 129.

Figure 129. NPD Thermionic Source Removal (1)

Thermionic Source Connector

Thermionic Source Support

Dowel

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

b. Using the T10 Torxhead screwdriver, remove the three T10 Torx screws from the
thermionic source assembly. See Figure 130.

Figure 130. NPD Thermionic Source Removal (2)

T10 Torx Screw

c. Gently lift up the thermionic source connector guiding the flexible cable from the
thermionic source support, then remove the thermionic source assembly from the
NPD body. Avoid bumping the bead on the sides of the collector. See Figure 131.

Figure 131. NPD Thermionic Source Removal (3)

Thermionic Source Connector

Thermionic Source Flexible Cable

Thermionic Source Assembly

Thermionic Source Support

NPD Body

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

12. Replace the thermionic source.

a. Remove the protective cap covering the new thermionic source.
b. Mount and guide the new source assembly on the NPD body proceeding in the
reverse order in which it was removed. Be careful not to bump the bead on the sides
of the body and collecting electrode.
c. Rotate and align the thermionic source connector, then tighten the dowel to fix the
connector to the support using the T6 Torxhead key. See Figure 132.

Figure 132. Replace NPD Thermionic Source (1)

Correct Alignment

Dowel

13. Reconnect and screw the straight jack connector of the collecting electrode cable to the
collecting electrode bulkhead jack. See Figure 133.

Figure 133. Replace NPD Thermionic Source (2)

Collecting Electrode Cable

Collecting Electrode Straight Jack Connector

14. Reconnect the source assembly cable to the NPD thermionic source, and twist the ring to
lock the connection. See Figure 134.

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Figure 134. Reconnect Source Assemble Cable

15. Close the module flap cover.

16. Plug in the signal cable into its contact on the detector module. See Figure 135.

Figure 135. Reconnect Signal Cable

17. Plug the power cable to the AC input connector of the NPD Thermionic Source Power
Module and to the wall outlet. The LED marked On blinks green.
If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
18. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
19. Set the make-up gas on.
20. Switch on the thermionic source. Hydrogen and air are automatically opened.
The LED marked On of the NPD Thermionic Source Power Module becomes solid
green.

21. With all gases on, heat the detector to 150 °C and hold for about 15 minutes. Increase the
temperature to 250 °C and hold for 15 minutes.

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

22. Increase the temperature to the operating value: 300 to 320 °C is recommended. Allow
15 minutes for equilibration.
23. Check the NPD leakage current. If > 2.0 pA, verify the installation.

Cleaning or Replacing the NPD Collecting Electrode

 To clean or replace the NPD collecting electrode

1. Put the GC in standby condition.

2. Switch off the thermionic source. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
4. Set the make-up gas off.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
6. Unplug the power cable from the AC input connector of the NPD Thermionic Source
Power Module and from the wall outlet.
If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
7. Unplug the signal cable from the detector module. See Figure 136.

Figure 136. Unplug NPD Signal Cable

8. Open the module flap cover.

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

9. Remove the thermionic source assembly cable.

a. Twist the ring to disconnect the thermionic source assembly cable.
b. Push and twist the lock so that the button slides up in the groove, then pull the cable
ends apart. See Figure 137.

Figure 137. NPD Thermionic Source Cable Removal

Thermionic Source Assembly Cable

Thermionic Source Assembly

10. Unscrew and pull out the straight jack connector of the collecting electrode cable from
the collecting electrode bulkhead jack. See Figure 138.

Figure 138. Collecting Electrode Cable Removal

Collecting Electrode Cable

Collecting Electrode Straight Jack

Connector

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

11. Remove the thermionic source.

CAUTION The thermionic source is delicate. Be careful not to break or crack the source.
When performing maintenance on the NPD, avoid touching the source with your fingers,
and prevent it from coming in contact with other surfaces.

Wear clean, lint-free gloves to prevent contamination of parts with dirt and skin oils.

a. Using a T6 Torxhead key, loosen the dowel which fix the thermionic source
connector to the thermionic source support. See Figure 139.

Figure 139. NPD Thermionic Source Removal (1)

Thermionic Source Connector

Thermionic Source Support

Dowel

b. Using the T10 Torxhead screwdriver, remove the three T10 Torx screws from the
thermionic source assembly. See Figure 140.

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 4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

Figure 140. NPD Thermionic Source Removal (2)

T10 Torx Screw

c. Gently lift up the thermionic source connector guiding the flexible cable from the
thermionic source support, then remove the thermionic source assemble from the
NPD body. Avoid bumping the bead on the sides of the collector. See Figure 141.

Figure 141. NPD Thermionic Source Removal (3)

Thermionic Source Connector

Thermionic Source Flexible Cable

Thermionic Source Assemble

Thermionic Source Support

NPD Body
Collecting Electrode Pin

12. Remove and clean the collecting electrode.

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4 Performing Detectors Routine Maintenance
Maintaining a Nitrogen Phosphorous Detector (NPD)

a. Using a 6-mm wrench, unscrew and remove the collecting electrode pin from its seat
on the detector body.
b. Using forceps or tweezers, extract the collecting electrode through the top of the
detector body. See Figure 142.

Figure 142. NPD Collecting Electrode Removal

Collector Electrode

c. Place the collecting electrode in the ultrasonic cleaner filled with liquid detergent.
d. Sonicate the collecting electrode for about five minutes.
e. Handling the collecting electrode with forceps or tweezers, rinse it using distilled
water, then methanol.
f. Place the collecting electrode on a paper towel and let it air dry.
Note If after cleaning the physical condition of the collecting electrode does not
permit its reuse, replace the electrode with a new one.

13. Reinstall the collecting electrode.

a. Place the cleaned (or a new) collecting electrode in its housing.
b. Using a 6-mm wrench, screw the collecting electrode pin into its seat on the detector
body.
c. Screw the straight plug crimp connector of the collecting electrode cable to the
collecting electrode bulkhead jack.
14. Reinstall the thermionic source.
a. Remove the protective cap covering the new thermionic source.

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Maintaining a Nitrogen Phosphorous Detector (NPD)

b. Mount and guide the new source assembly on the NPD body proceeding in the
reverse order in which it was removed. Be careful not to bump the bead on the sides
of the body and collecting electrode.
c. Rotate and align the thermionic source connector, then tighten the dowel to fix the
connector to the support using the T6 Torxhead key. See Figure 143.

Figure 143. Replace NPD Thermionic Source (1)

Correct Alignment

Dowel

15. Reconnect and screw the straight jack connector of the collecting electrode cable to the
collecting electrode bulkhead jack. See Figure 144.

Figure 144. Replace NPD Thermionic Source (2)

Collecting Electrode Cable

Collecting Electrode Straight Jack Connector

16. Reconnect the source assemble cable to the NPD cable and twist the ring to lock the
connection. See Figure 145.

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Maintaining a Nitrogen Phosphorous Detector (NPD)

Figure 145. Reconnect Source Assemble Cable

17. Close the module flap cover.

18. Plug in the signal cable into its contact on the detector module. See Figure 146.

Figure 146. Reconnect Signal Cable

19. Plug the power cable to the AC input connector of the NPD Thermionic Source Power
Module and to the wall outlet. The LED marked On blinks green.
If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
20. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
21. Turn the make-up gas on.
22. Heat the detector at the working temperature.
23. Switch on the thermionic source. Hydrogen and air are automatically opened.
The LED marked On of the NPD Thermionic Source Power Module becomes solid
green.

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 4 Performing Detectors Routine Maintenance
Maintaining a Thermal Conductivity Detector (TCD)

Maintaining a Thermal Conductivity Detector (TCD)

This section provides instructions for maintaining a Thermal Conductivity Detector (TCD).
The component of module and detector are shown in Figure 147.

Figure 147. TCD Module Components

Module Flap Cover

Vent

Heat Sink

Cell Upper Insulating Cover

Cell Lower Insulating Cover

Bottom Fittings

The same routine maintenance is applied to the In-Series Connection TCD Module.
See Figure 148.

Figure 148. In-Series Connection TCD Module

Vent Line Metal Tubing

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4 Performing Detectors Routine Maintenance
Maintaining a Thermal Conductivity Detector (TCD)

The TCD detector does not usually need maintenance. Nevertheless, if you follow a few
simple precautions, you will avoid problems and prolong the detector’s lifetime. Pay a special
attention to avoid contamination or damage to the filaments.

The detector must be returned to the factory when filament replacement is required.

CAUTION Do not turn the filament on until the carrier gas is flowing through the
detector.

Do not shut off or disconnect the carrier gas when the detector is hot, even if the unit is
turned off.

Follow these simple rules:

• Avoid switching on the filaments when unnecessary. Doing so considerably reduces the
filament’s lifetime.
• Avoid injecting samples that contain halogenated or acid compounds at high
concentrations.
• Ensure that oxygen (air) cannot enter into the filament’s cells. Oxidation will irreversibly
damage the filaments. Install traps for moisture and oxygen on the gas lines to reduce the
risk of contamination.
• Turn off the filament before disconnecting the column from the detector. When the
column is disconnected, air will enter into the cell and the filament, if powered on, will be
damaged.
• Turn on the filament only if the column is connected. It is a good practice to let the gas
flow through the cells for 5-10 minutes before powering the filaments.

See Bake-out Procedure, Measuring the Carrier Gas Flow Rate, and Shutting Down the TCD
for additional information.

Bake-out Procedure
Under normal conditions, the TCD requires no routine maintenance. However, if the
detector is exposed to chemicals which may condense or polymerize within the detector and
adversely affect performance, an attempt of filament cleaning could be baked out at high
temperatures (up to 300°C). Inert carrier gas flow should be maintained during the
reconditioning procedure.
Filament temperature should also be increased to approximate the setpoint of the bake-out
temperature.
If a 24-hour bake-out is not sufficient to remove the contamination, the unit must be
returned to the factory for disassembly and cleaning.

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Measuring the Carrier Gas Flow Rate

While the measuring of the carrier gas flow rate through the standard TCD detector is
performed simply connecting the flow-meter to the vent outlet, the TCD In-series detector
requires the use of the column- flowmeter connector.

 To measure the TCD In-Series carrier gas flow rate

Carefully push the vent line metal tubing end into the flow meter section of the column-
flowmeter connector. See Figure 149.

Figure 149. Column Flowmeter Connector

A B C
Vent Line Metal Tubing

To flowmeter

Connect the flowmeter to the dedicated fitting on the column-flowmeter connector, then
measure the carrier gas flow rate.

Shutting Down the TCD

To shut down the detector at the end of the analytical cycle:
1. Turn off the filaments.
2. Reduce the carrier gas flow to 50% of the normal operating flow to conserve gas supplies.

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4 Performing Detectors Routine Maintenance
Maintaining an Electron Capture Detector (ECD)

Maintaining an Electron Capture Detector (ECD)

This section provides instructions for maintaining an Electron Capture Detector (ECD).
The component of module and detector are shown in Figure 150 and Figure 151.

Figure 150. ECD Module Components

Module Flap Cover

Signal Cable
Excitation Cable

Collecting Electrode
(Anode) Screw Plug
See Figure 151
Chimney
Detector Cell

Insulating Cover

Figure 151. Collecting Electrode (Anode) Screw Plug

Collecting Electrode
(Anode) Screw Plug

The ECD detector periodic maintenance includes:

• Cleaning the collecting electrode (Anode)
We suggest cleaning the collecting electrode annually or when a high noise baseline is
found producing detector contamination. Replace the collecting electrode if necessary.
See “Cleaning or Replacing the Collecting Electrode (Anode)” on page 169.

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Maintaining an Electron Capture Detector (ECD)

Before maintaining the detector, read the following precautions and notes:

CAUTION The Electron Capture Detector contains a 63Ni beta-emitting radioactive

source at 370 MBq (10 mCi). The detector should never be opened or handled by the
user. Any maintenance or service operations involving even partial disassembling of the
detector must be performed ONLY by qualified personnel at a laboratory expressly
authorized by Thermo Fisher Scientific and specifically licensed to handle radioactive
material.
IMPORTANT For customers within the jurisdiction of the United States Nuclear
Regulatory Commission (US NRC), you may find a listing of the agreement states and
the current contact information for the regulators covering both Generally and
Specifically licensed devices like an ECD at http://nrc-stp.ornl.gov/rulemaking.html.
This information is maintained by the US NRC.
IMPORTANT For customers within the jurisdiction of the Canadian Nuclear Safety
Commission, you may find current contact information for the regulators at
http://www.nuclearsafety.gc.ca
IMPORTANT The recommended working life of the detector is 15 years, after which the
user should arrange for the radioactive source to be inspected and assessed by a qualified
authority to extend its working life, or dispose of the product through a suitable disposal
route.
IMPORTANT According to International, USA, and Canada licenses, the appropriate
Radioactivity Data labels are applied on the ECD detector module.
International US General
License License

Common
Label US Specific
License

Canada
License

Exclusively for ECD detector module used in China, the Caution Radioactive Material
Ni63 labels are replaced by the followings:

Caution Radioactive Material

Labels for China

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4 Performing Detectors Routine Maintenance
Maintaining an Electron Capture Detector (ECD)

WARNING All the operations must be carrier out at low temperature to avoid burns.
Therefore, before conducting maintenance, cool the detector to room temperature.

Materials needed to maintain an ECD detector

Ultrasonic cleaner
GC-grade hexane
GC-grade toluene
Fine emery paper
Little flathead screwdriver
T20 Torxhead screwdriver
Forceps or tweezers
Collecting electrode (Anode) if necessary

Detector Chemical Contamination

The ECD, if properly used, has a good resistance against chemical contamination. However,
some critical operating conditions may cause, over time, contamination of the collecting
electrode (anode). This contamination is highlighted by an excessive increase of the base
frequency, and a baseline drift when the reference current or the pulse amplitude is changed.
The collecting electrode can be easily removed and cleaned without disturbing the radioactive
source.

Detector contamination could be indicated by the following effects in the chromatogram:

• Reduced signal to noise ratio
• High-noise baseline (high frequency)
• Baseline drift with changing pulse voltage
• Negative dips after peaks

If chemical contamination of the whole cell is suspected proceed as follows:

1. Heat the ECD at the maximum operating temperature with carrier and make-up gases
flowing through the detector (thermal cleaning).
2. Follows the decontamination process by monitoring the base frequency. Initially the
frequency value tends to increase to very high values, and then it decreases to acceptable
ones.

If irreversible contamination of the cell is suspected please contact your local Thermo Fisher
Scientific Technical Service office.

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Wipe Test
Before leaving the factory, each ECD is leak tested for surface radio contamination by means
of the Wipe Test method. Each detector is provided with a Wipe Test Certificate reporting
the results of the values found and the procedure followed.

IMPORTANT The users of the ECD detector in the United States are required to perform
a Wipe Test on their ECD at intervals not exceeding 6 months, following the reported
procedure. For other countries, please refer to the appropriate agency for equivalent
requirements.

Cleaning or Replacing the Collecting Electrode (Anode)

 To clean or replace the collecting electrode

1. Put the GC in standby condition.

2. Cool the detector to room temperature.
3. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.

6. Open the module flap cover.

7. Disconnects the signal and excitation cables from their contacts on the cell top cover.
See Figure 152.

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Maintaining an Electron Capture Detector (ECD)

Figure 152. ECD Cables Disconnection

Excitation Cable

Signal Cable

a. Unscrew and pull out the straight plug crimp connector of the signal cable from the
collecting electrode (anode) bulkhead jack.
b. Pull out the quick coupling straight jack connector of the excitation cable from the
polarizing electrode bulkhead jack.
c. Carefully move the cables in order to have free space for handling the detector.
8. Remove and clean the collecting electrode (anode).
a. Unscrew the screw plug with its seal hiding the collecting electrode (anode) using the
T20 Torxhead screwdriver.
b. Using a little flathead screwdriver, unscrew and remove the collecting electrode
(anode). See Figure 153.

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Figure 153. Collecting Electrode (Anode) Removal

Collecting Electrode
Screw Plug
Seal

Collector Electrode

c. Place the collecting electrode in the ultrasonic cleaner filled with liquid detergent,
and clean it for about five minutes.
d. Handling the collecting electrode (anode) with forceps or tweezers, rinse it using
distilled water, then methanol.
e. Place the collecting electrode (anode) on a paper towel and let it air dry.
Note If after cleaning the physical condition of the collecting electrode (anode)
does not permit its reuse, replace the electrode with a new one.

9. Reinstall the collecting electrode (anode).

a. Screw the cleaned, or a new collecting electrode (anode) into its housing. Screw the
hiding screw plug and its seal.
10. Reconnect signal and excitation cables.
11. Close the module flap cover.
12. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
13. Power on the GC.
a. Plug the power cable to the AC Input connector on the back of the GC, and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
14. Set the make-up gas on.
15. Set the normal detector working conditions.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Maintaining a Flame Photometric Detector (FPD)

This section provides instructions for maintaining a Flame Photometric Detector (FPD).
See Figure 154.

Figure 154. FPD Control Module

Photomultiplier Assembly

FPD Cell Assembly

FPD Control Module

The control module and detector cell assembly components are shown in Figure 155,
Figure 156, and Figure 157.
Figure 155. FPD Control Module Components
Ignition/heating Cable

Excitation Voltage and Signal Cables

FPD Jet
Aluminium O-Ring
FPD Detector Base Body

Module Flap Cover

Module Cover

Note The signal, excitation voltage, and ignition/heating cables will be not shown in the
next illustrations for graphic convenience.

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Figure 156. FPD Detector Assembly Components

Photomultiplier Assembly Detector Cell

Chimney Cap

Photomultiplier Tube Housing

Photomultiplier Tube Holder

Knurled Nut
FPD Blocking Nut

Mirror Plug Assembly

Ignition/heating Cable Connection

Signal Cable Connection

Excitation Voltage Cable Connection

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Maintaining a Flame Photometric Detector (FPD)

Figure 157. FPD Cell Assembly Components

FPD Cell Assembly

Photomultiplier Assembly
Cover
Mirror Plug
Graphite Seal
Detector Body
Chimney Cap
Chimney

Flame-side Heat Shield

Graphite Seal
Spacer
Flange

Filter-side Heat Shield

Viton® O-ring

Filter Support
Viton® O-ring
Interferential Filter

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

To ensure optimum performance of the FPD, you must keep it clean and free of dust and
deposits. Symptoms such as reduced sensitivity and increased noise indicate that detector
cleaning could be necessary.

The FPD detector periodic maintenance includes:

• Installing the FPD detector
See “Installing the FPD Detector” on page 176.
• Removing the FPD detector
See “Removing the FPD Detector” on page 178.
• Cleaning or replacing the FPD jet
You should clean the jet semiannually when you analyze particularly dirty compounds
that the flame does not burn properly.
See “Cleaning or Replacing the FPD Jet” on page 179.
• Cleaning or replacing the FPD interferential filter
See “Cleaning or Replacing the FPD Interferential Filter” on page 183.
• Replacing the FPD ignition glow-plug
This operation is NOT part on the ordinary maintenance. The flame ignition element
must be replaced only when defective.
See “Replacing the FPD Ignition Glow-plug” on page 187.

Before maintaining the detector, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed to maintain a FPD detector

FPD fixing tool
Gloves
Ultrasonic cleaner
Liquid detergent
GC-grade methanol
Distilled water

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Materials needed to maintain a FPD detector

Paper towels
Forceps or tweezers
FPD Jet (if necessary)
Interferential filter for sulphur PN 28107000 or PN 19050785, (if necessary)
Interferential filter for phosphorous PN 28107100 or PN 19050785, (if necessary)
Interferential filter for tin PN 28107001 (optional), (if necessary)
FPD Ignition Glow-plug
T6 Torxhead key
T10 Torxhead screwdriver
5 mm wrench
5/16-in tube wrench

Note For maintaining or replacing any other component not listed in this section, see
Chapter 7, “Detectors Advanced Maintenance.”
• “Measuring the FPD Gas Flows” on page 293
• “Cleaning or Replacing the FPD Mirror Metal Plug” on page 295
• “Cleaning or Replacing the FPD Filter-side Heat Shields” on page 300
• “Cleaning or Replacing the FPD Flame-side Heat Shields” on page 308
• “Replacing the FPD Photomultiplier Tube” on page 317

Installing the FPD Detector

Before proceeding the installation of the FPD detector assembly, make sure that the FPD
control module is properly installed into the back detector housing.

 To install the FPD detector assembly on the FPD detector base body

1. Place the jet into the detector base body housing and tighten it. Ensure the jet is perfectly
vertically aligned to avoid damage. Figure 158.
2. Place the FPD on the detector base body, ensuring that the aluminium ring has been
inserted in the correct position. Tighten the fixing nut by using the FPD fixing tool.
See Figure 158.

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Figure 158. FPD Installation

FPD Detector Assembly

FPD Fixing Nut

Jet
Aluminium Ring
Detector Base

FPD Control Module FPD Fixing Tool

3. Carefully, connect the signal, excitation voltage, and ignition/heating cables, coming from
the FPD control module, to the FPD detector. See Figure 159.
Figure 159. FPD Cables Connection

Ignition/Heating Cable

Signal Cable

Excitation
Voltage
Cable

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Removing the FPD Detector

 To remove the FPD detector assembly from the FPD detector base body

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detector from the base.
a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 160

Figure 160. FPD Cable Disconnection

Ignition/Heating Cable

Signal Cable

Excitation
Voltage
Cable

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 161.

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Maintaining a Flame Photometric Detector (FPD)

Figure 161. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

Note Do not lose the aluminium ring inserted between the detector head and
the base body.

Cleaning or Replacing the FPD Jet

 To clean or replace the FPD jet

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.

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Maintaining a Flame Photometric Detector (FPD)

6. Remove the FPD detector from the base.

a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 162.

Figure 162. FPD Cable Disconnection

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 163.

Figure 163. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Note Do not lose the aluminium ring inserted between the detector head and the
base body.

7. Clean the FPD Jet.

a. Using the 5 mm wrench provided with the GC, loosen the jet and remove it from the
detector base body. See Figure 164.

Figure 164. FPD Jet Removal

b. Place the jet in the ultrasonic cleaner filled with liquid detergent and clean it for
about five minutes.
c. Handling the jet with forceps or tweezers, rinse the jet with distilled water, then with
methanol.
d. Place the jet on a paper towel and let it air dry. When the jet is dry, insert the jet into
the detector base body and tighten it. See Figure 165.

Figure 165. FPD Jet Reinstallation

Note If after cleaning the physical condition of the jet does not permit its reuse,
replace the jet with a new one.

8. Reinstall the FPD detector on the base.

a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut. See Figure 166.

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Maintaining a Flame Photometric Detector (FPD)

Figure 166. FPD Detector Reinstallation

FPD Fixing Nut

Aluminium Ring

b. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 167.

Figure 167. FPD Cables Connection

9. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
10. Power on the GC.

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Maintaining a Flame Photometric Detector (FPD)

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
11. Set the normal working conditions.

Cleaning or Replacing the FPD Interferential Filter

Before maintaining the detector, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

 To clean or replace the FPD interferential filter

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Disconnect the signal, excitation voltage, and ignition/heating cables from the detector.
See Figure 168.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Figure 168. FPD Cables Disconnection

7. Remove the photomultiplier assembly and the filter. See Figure 169.

Figure 169. Photomultiplier Assembly Removal

Knurled Nut

a. Loosen the knurled nut that fixes the photomultiplier assembly and remove it from
the detector body.

CAUTION The photomultiplier tube could damage if exposed to ambient light with the
excitation voltage On. Make sure the power supply has been switched off before
disconnecting the tube from the detector body.

b. Remove the interferential filter from its housing, handling it very gently.
Keep it using a clean paper towel. See Figure 170.

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Maintaining a Flame Photometric Detector (FPD)

Figure 170. Interferential Filter Removal

CAUTION Filters are fragile. Pay attention not to let the filter fall down and damage.

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first remove the spacer, and then the interferential filter.

Interferential Filter

Spacer

Keep them using a clean paper towel.

8. Clean the filter.

a. Using a clean paper towel, clean the filter on both faces.

CAUTION Avoid touching the filter with your fingers. If you see fingertips on the filter,
clean it using GC-grade methanol and air dry before remounting.

9. Reassembly the filter and the photomultiplier assembly.

a. Insert the cleaned filter or a new filter into its housing. The mirror face must be
oriented towards the flame. See Figure 171.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Figure 171. Filter Reinstallation

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first insert the filter, and then the spacer.

Interferential Filter

Spacer

b. Reassembly the photomultiplier assembly and the detector body, then fix them
together tightening the knurled nut. See Figure 172.

Figure 172. Photomultiplier Assembly Remounting

10. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 173.

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Figure 173. FPD Cables Connection

11. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Set the normal working conditions.

Replacing the FPD Ignition Glow-plug

 To replace the FPD ignition glow-plug

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

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Maintaining a Flame Photometric Detector (FPD)

5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detector from the base.
a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 174.

Figure 174. FPD Cables Disconnection

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 175.
Figure 175. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

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 4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Note Do not lose the aluminium ring inserted between the detector head and the
base body.

7. Remove the covers of the FPD detector body.

CAUTION Removing the covers, pay attention to the heater element and the temperature
probe.

a. Using a 5/16-in tube wrench, remove the two screws on the top of the detector body,
and the front and back screws on the lower part of the detector body. See Figure 176.

Figure 176. Detector Body Dismounting (1)

Top Screws
Right Cover

Front Screw
Back Screw

b. Remove the left and right covers of the FPD detector body and the insulating
material. The glow-plug is now accessible. See Figure 177.

Figure 177. Detector Body Dismounting (2)

Right Cover
Insulating
Material

Insulating
Material

Left Cover

Glow-plug Cable
Terminal body

8. Replace the defective glow-plug. See Figure 178.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

Figure 178. Glow-plug Replacing

Glow-plug
Glow-plug Dowel Pin
Socket Set Screw

Heater Element
temperature probe

a. Loosen the glow-plug cable socket set screw using a T6 Torxhead screwdriver.
Carefully pull out the terminal body of the glow-plug cable from the dowel pin on
the glow-plug element.
b. Using an 8-mm wrench, unscrew and remove the defective glow-plug with its washer.
c. Replace the glow-plug with a new one interposing the new washer.
9. Reinstall the left and right covers of the FPD detector body with the insulating material
in the original position, and fix them with the four fixing screws. See Figure 179.

CAUTION Reinstalling the covers, pay attention to the heater element and the
temperature probe.

Figure 179. Detector Body Remounting

Top Screws

Right Cover

Front Screw
Back Screw

10. Reinstall the FPD detector on the base.

a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut. See Figure 180.

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Maintaining a Flame Photometric Detector (FPD)

Figure 180. FPD Detector Reinstallation

FPD Fixing Nut

Aluminium Ring

b. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 181.

Figure 181. FPD Cables Connection

11. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
12. Power on the GC.

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4 Performing Detectors Routine Maintenance
Maintaining a Flame Photometric Detector (FPD)

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Set the normal detector working conditions.
14. Ignite the flame.

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 4 Performing Detectors Routine Maintenance
Maintaining a Pulsed Discharge Detector (PDD)

Maintaining a Pulsed Discharge Detector (PDD)

The Pulsed Discharge Detector (PDD) does not require maintenance.

WARNING Under no circumstances should the detector be disassembled.

The components of the detector are assembled with special tooling and held under
considerable force. Disassembling of the detector may present a safety hazard and will
result in its destruction.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 193
 5

GC Main Frame Advanced Maintenance

This chapter describes TRACE 1300/TRACE 1310 GC components that do not require
routine maintenance, but they need to be removed or replaced.

Contents
• Removing/Replacing the GC Top Cover
• Removing/Replacing the GC Left Side Panel
• Removing/Replacing the GC Right Side Panel
• Removing/Replacing the GC Back Cover
• Removing/Replacing the GC Front Door Cover
• Removing/Replacing the Electronic Module
• Replacing the Oven Heater Baffle
• Replacing the Oven Heater Temperature Sensor
• Replacing the Oven Motor
• Replacing the Flap Motor

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Removing/Replacing the GC Top Cover

Removing/Replacing the GC Top Cover

Perform this operation for accessing the internal compartments of the GC.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To remove/replace the top cover accessing the top parts of the GC

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Close the gas supplies.
b. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
c. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the autosampler if present.
6. Remove the cover.
a. Using a T20 Torxhead screwdriver, unscrew the four screws that held it in place.
See Figure 182 and Figure 183.

Figure 182. Top Cover Removal (1)

Top Cover Screws

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 5 GC Main Frame Advanced Maintenance
Removing/Replacing the GC Left Side Panel

b. Lift the top panel up and off the GC.

Figure 183. Top Cover Removal (2)

7. Replace the top cover proceeding in the reverse order in which it was removed.

Removing/Replacing the GC Left Side Panel

 To remove the left side panel with the side panel molded of the GC

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the GC Left Side Panel

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the panel.
a. Open the front door of the GC. Using a T20 Torxhead screwdriver, unscrew the left
side panel screw from the interior front panel. See Figure 184.

Figure 184. Left Side Panel Fixing Screw

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Removing/Replacing the GC Right Side Panel

b. Slide the panel towards the back of the instrument up to the stop.
c. Remove the panel pulling it outwards being aware that the ground wire is attached to
the panel. See Figure 185.

Figure 185. Left Panel Removal

d. Unplug the ground wire from the panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

6. Replace the panel.

a. Plug the ground wire to the panel.
b. Reinstall the panel proceeding in the reverse order in which it was removed.

Removing/Replacing the GC Right Side Panel

 To remove the right side panel with the side panel molded of the GC

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the GC Right Side Panel

4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the AI/AS autosampler, if present.
6. Remove the panel.
a. Open the front door of the GC. Using a T20 Torxhead screwdriver, unscrew the
right side panel screw from the interior front panel. See Figure 186.

Figure 186. Right Panel Fixing Screw

b. Slide the panel towards the back of the instrument up to the stop.
c. Remove the panel pulling it outwards being aware that the ground wire is attached to
the panel. See Figure 187.

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Removing/Replacing the GC Back Cover

Figure 187. Right Panel Removal

d. Unplug the ground wire from the panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

7. Replace the panel.

a. Plug the ground wire to the panel.
b. Reinstall the panel proceeding in the reverse order in which it was removed.

Removing/Replacing the GC Back Cover

Remove the back cover for accessing the compartment on the back of the GC.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To remove the back cover

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the GC Back Cover

4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the cover.
a. Using a T20 Torxhead screwdriver, unscrew the four screws that secure the back cover
to the GC. See Figure 188.

Figure 188. Back Cover Removal

Back Cover Fixing Screws

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back cover. See Figure 189.

Figure 189. Back Cover

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Removing/Replacing the GC Front Door Cover

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

6. Reconnect the ground wire to the back cover terminal.

7. Replace the cover proceeding in the reverse order in which it was removed.

Removing/Replacing the GC Front Door Cover

This section provides the instruction for removing or replacing the cover of the front door.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To remove the cover of the front door of the GC

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the door cover.
a. Open the front door and look for the fixing screws that secure the cover and the door
handle to the door. See Figure 190.

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the GC Front Door Cover

Figure 190. Front Door Back View

Upper Fixing Screws

Door Handle Fixing

Screw

Lower Fixing Screws

Spacer

b. Using a T20 Torxhead screwdriver remove the screw that secure the door handle to
the front door.
c. Pull the door handle out from the front door. See Figure 191.

IMPORTANT Save the door handle because it must be reused.

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Removing/Replacing the GC Front Door Cover

Figure 191. Door Handle Removal

Door Handle

d. Using a T20 Torxhead screwdriver remove the three upper and the three lower screws
that secure the cover to the front door. See Figure 192.
Note The lower screw on the lower right corner is screwed into a spacer.

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the GC Front Door Cover

Figure 192. Door Cover Fixing Screws Removal

Upper Fixing Screws

Lower Fixing Screws

e. Carefully pull the door cover off. Pay attention to the cables that connect the status
panel to the internal section of the door. See Figure 193.

Figure 193. Front Door Removal

Door Cover

Front Door

f. Disconnect the cables from the connector located on the front of the door.

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Removing/Replacing the GC Front Door Cover

• See Figure 194 in case of TRACE 1300.

• See Figure 195 in case of TRACE 1310.

Figure 194. TRACE 1300 Front Door Cables Removal

Flat Cables

Figure 195. TRACE 1310 Front Door Cables Removal

Flat Cable

Ground Cable

6. Replace the cover proceeding in the reverse order in which it was removed.
7. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the Electronic Module

8. Power on the GC.

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
9. Set the normal injector, detector, and GC working conditions.

Removing/Replacing the Electronic Module

The Electronic Module contains power and electronic circuits for the control of the
instrument. A proper module is installed according to 120 Vac or 230 Vac power supply.

WARNING This operation must be carried out only by authorized and trained Thermo
Fisher Scientific technical personnel.

 To remove/replace the electronic module

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Unplug the cables connected to the external interface of the Electronic Module.
6. Remove the autosampler if present.
7. Remove the top cover.
a. Use a T20 Torxhead screwdriver to remove the screws at the sides of the top cover.
See Figure 196.

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Removing/Replacing the Electronic Module

Figure 196. Top Cover Removal (1)

Top Cover Fixing Screws

b. Slide the top panel toward the back of the instrument and lift it off. See Figure 197.

Figure 197. Top Cover Removal (2)

Electronic Module

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5 GC Main Frame Advanced Maintenance
Removing/Replacing the Electronic Module

8. Remove the Electronic Module.

a. Using the two handles on the top cover, pull out module from its housing.
See Figure 198.

Figure 198. Electronic Module Extraction

Handles

Electronic Module Housing

9. Replace the Electronic Module.

a. Guide the electronic module into its housing.
b. Push down the module. Be sure to plug the terminal contacts of the mother board
into the two slots of the backplane board. See Figure 199 and Figure 200.

Figure 199. Electronic Module Replacement (1)

Mother Board Connectors

Backplane Board Connectors

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Replacing the Oven Heater Baffle

Figure 200. Electronic Module Replacement (2)

10. Reinstall and fix the top cover of the GC.

11. Reinstall the autosampler if present.
12. Plug the cables connected to the external interface of the Electronic Module.
13. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
14. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
15. Set the normal injector, detector, and GC working conditions.

Replacing the Oven Heater Baffle

Removing the oven heater requires replacing the complete oven heater baffle. This includes
the plate, heaters, and temperature sensor.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

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5 GC Main Frame Advanced Maintenance
Replacing the Oven Heater Baffle

 To replace the oven heater baffle

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the back cover.
a. Using a T20 Torxhead screwdriver, remove the four screws that secure the back cover
to the GC. See Figure 201.

Figure 201. Back Cover Removal

Back Cover Fixing Screws

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

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Replacing the Oven Heater Baffle

6. Remove the analytical column.

a. Open the front door of the GC.
b. Loosen the retaining nut from the injector and detector fitting on the upper interior
wall of the GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector and the detector.
7. Remove the oven heater baffle.
The oven heater baffle is attached to the oven wall by four adjustable tabs. These tabs are
attached to the oven heater baffle with four Torx screws. See Figure 202.

Figure 202. Oven Heater Baffle

Torx Screws

Oven Heater
Baffle

The heater and temperature sensor wires are connected to the backplane board passing
through the three pipes provided on the oven back wall.
• The temperature sensor wire is plugged into the connector marked J4 (Oven PT100)
• The heater wires are plugged into the connector J23, J24, and J25 (Oven Heater).
The pipes are plugged by insulation material. See Figure 203.

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5 GC Main Frame Advanced Maintenance
Replacing the Oven Heater Baffle

Figure 203. Pipes

Backplane Board

Pipes

Oven Motor

a. Unplug the heater and temperature sensor wires from the relevant connector on the
backplane board.
b. Loosen and remove the four Torx screws that secure the tabs to the oven heater baffle.
See Figure 202.
8. Pull out the oven heater baffle from the oven, paying attention to guide the heater and
temperature sensor wires through the three pipes on the back oven wall.

IMPORTANT During this operation, parts of the insulation material drops down into the
oven. This material is reused later when a new Oven Heater Baffle is installed.

9. Replace the oven heater baffle in the reverse order in which it was removed.
a. Guide the heater and temperature sensor wires through the pipes provided on the
oven back wall.
b. Reconnect the temperature sensor and heater wires to the relevant connector J4, J23,
J24, and J25 on the backplane board.
c. Plug the pipes with the insulation material.
d. Secure the oven heater baffle to the oven wall with the four Torx screws in the tabs.
The tab adjustment screws should be loose. Carefully center the oven heater baffle
inside of the oven. Be sure that the center hole in the oven heater baffle screen is
centered over the blower motor shaft. The tolerance is very small.
The oven heater baffle will not move left or right, nor up or down more than a couple
of millimeters. However, be sure that it is centered in the oven over the blower wheel.
This will ensure that whenever the GC is turned on, the blower wheel will not be
touching the heater element mounted to the oven heater baffle.

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Replacing the Oven Heater Temperature Sensor

10. Reinstall the back cover.

a. Reconnect the ground wire to the back cover terminal.
b. Replace the cover proceeding in the reverse order in which it was removed.
11. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Close the front door of the GC. Be sure that the oven blower is running.
a. Verify that the heater heats, and the fan rotates without contacting the heater
element.
14. Open the front door of the GC.
15. Reinstall the column.
16. Set the normal injector, detector and GC working conditions.

Replacing the Oven Heater Temperature Sensor

Removing the oven heater temperature sensor requires removing the complete oven heater
baffle.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To replace the oven heater temperature sensor

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

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5 GC Main Frame Advanced Maintenance
Replacing the Oven Heater Temperature Sensor

c. If external modules are present, unplug the power cable from the AC Input connector
of each external module, and from the wall outlet.
4. Remove the back cover.
a. Using a T20 Torxhead screwdriver remove the four screws that secure the back cover
to the GC. See Figure 204.

Figure 204. Back Cover Removal

Back Cover Fixing Screws

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

5. Remove the analytical column.

a. Open the front door of the GC.
b. Loosen the retaining nut from the injector and detector fitting on the upper interior
wall of the GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector and the detector.
6. Remove the oven heater baffle.
The oven heater baffle is attached to the oven wall by four adjustable tabs. These tabs are
attached to the oven heater baffle with four Torx screws. See Figure 205.

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Replacing the Oven Heater Temperature Sensor

Figure 205. Oven Heater Baffle

Torx Screws

Oven Heater
Baffle

The heater and temperature sensor wires are connected to the backplane board passing
through the three pipes provided on the oven back wall.
• The temperature sensor wire is plugged into the connector marked J4 (Oven PT100)
• The heater wires are plugged into the connector J23, J24, and J25 (Oven Heater).
The pipes are plugged by insulation material. See Figure 206.

Figure 206. Pipes

Backplane Board

Pipes

Oven Motor

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5 GC Main Frame Advanced Maintenance
Replacing the Oven Heater Temperature Sensor

a. Unplug the heater and temperature sensor wires from the relevant connector on the
backplane board.
b. Loosen and remove the four Torx screws that secure the tabs to the oven heater baffle.
See Figure 205 on page 217.
c. Place the heater baffle on the roof of the oven.

IMPORTANT During this operation, parts of the insulation material drops down into the
oven. This material is reused later when a new Oven Heater Baffle is installed.

7. Remove the oven heater temperature sensor. See Figure 207.

Figure 207. Oven Heater Temperature Sensor

Temperature Sensor

Clamps

a. Loosen the two clamps that attaches the oven heater temperature sensor to the oven
heater baffle.
b. Remove the temperature sensor.

WARNING Pay attention to not damage the sensitive element.

8. Replace the oven heater temperature sensor in the reverse order in which it was removed.
9. Replace the oven heater baffle in the reverse order in which it was removed.
a. Guide the heater and temperature sensor wires through the pipes provided on the
oven back wall.
b. Reconnect the temperature sensor and heater wires to the relevant connector J4, J23,
J24, and J25 on the backplane board.

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Replacing the Oven Motor

c. Plug the pipes with the insulation material.

d. Secure the oven heater baffle to the oven wall with the four Torx screws in the tabs.
The tab adjustment screws should be loose. Carefully center the oven heater baffle
inside of the oven. Be sure that the center hole in the oven heater baffle screen is
centered over the blower motor shaft. The tolerance is very small. The oven heater
baffle will not move left or right, nor up or down more than a couple of millimeters.
However, be sure that it is centered in the oven over the blower wheel. This will
ensure that whenever the GC is turned on, the blower wheel will not be touching the
heater element mounted to the oven heater baffle.
10. Reinstall the back cover.
a. Reconnect the ground wire to the back cover terminal.
b. Replace the cover proceeding in the reverse order in which it was removed.
11. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Close the front door of the GC. Be sure that the oven blower is running.
a. Verify that the heater heats, and the fan rotates without contacting the heater
element.
14. Open the front door of the GC.
15. Reinstall the column.
16. Set the normal detector and GC working conditions.

Replacing the Oven Motor

Removing the oven motor requires the removal of several parts and assemblies.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To replace the oven motor

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

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5 GC Main Frame Advanced Maintenance
Replacing the Oven Motor

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the back cover.
a. Using a T20 Torxhead screwdriver remove the four screws that secure the back cover
to the GC. See Figure 208.

Figure 208. Back Cover Removal

Back Cover Fixing Screws

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

6. Remove the analytical column.

a. Open the front door of the GC.

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Replacing the Oven Motor

b. Loosen the retaining nut from the injector and detector fitting on the upper interior
wall of the GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector and the detector.
7. Remove the oven heater baffle.
The oven heater baffle is attached to the oven wall by four adjustable tabs. These tabs are
attached to the oven heater baffle with four Torx screws. See Figure 209.

Figure 209. Oven Heater Baffle

Torx Screws

Oven Heater
Baffle

The heater and temperature sensor wires are connected to the backplane board passing
through the three pipes provided on the oven back wall.
• The temperature sensor wire is plugged into the connector marked J4 (Oven PT100)
• The heater wires are plugged into the connector J23, J24, and J25 (Oven Heater).
The pipes are plugged by insulation material. See Figure 210.

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5 GC Main Frame Advanced Maintenance
Replacing the Oven Motor

Figure 210. Pipes

Backplane Board

Pipes

Oven Motor

a. Unplug the heater and temperature sensor wires from the relevant connector on the
backplane board.
b. Loosen and remove the four Torx screws that secure the tabs to the oven heater baffle.
See Figure 209 on page 221.
c. Place the heater baffle on the roof of the oven.

IMPORTANT During this operation, parts of the insulation material drops down into the
oven. This material is reused later when a new Oven Heater Baffle is installed.

8. Remove the blower fan.

The oven blower fan is attached to the shaft of the oven blower motor with an Allen
screw. See Figure 211.

Figure 211. Oven Blower Fan

Set Screw

Oven Blower Fan

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 5 GC Main Frame Advanced Maintenance
Replacing the Oven Motor

a. Loosen the Allen screw. Carefully remove the oven fan from the blower motor shaft.

9. Remove the blower motor.

a. The blower motor plugs into the connector marked J7 (Motor fan) on the Backplane
board. Unplug the blower motor electrical plug by pushing down on the plug tab and
pulling out.
b. The ground strap from the motor is secured to chassis ground on a terminal just
below the starting capacitor. Locate this terminal.
c. Remove the terminal nut using a 4-mm nut driver; or, unplug the connector. Remove
the ground wire.
d. The blower motor is secured to the back of the oven wall with three #4 self-locking
nuts. Remove these nuts using the same 4-mm nut driver. See Figure 212.

Figure 212. Removal the Oven Blower Motor

Self Locking Nuts

e. Locate and note the orientation of the six shoulder washers on each side of the three
grommets, before removing the blower motor.
f. Locate the three flat washers between the locking nut and the shoulder washers.
Remove and retain these washers for reassembly.
g. Remove the blower motor from the oven by pulling outward.
10. Assemble the oven motor
The replacement blower motor contains the mounting brackets with the three grommets
in place. The existing blower motor will have a total of six shoulder washers.
Three shoulder washers are installed on each side of the grommets.
a. Remove the all of the shoulder washers from the old blower motor.
b. Place three of the shoulder washers over the mounting studs located on the back oven
wall.

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Replacing the Oven Motor

c. Install the motor on the studs.

d. Place the remaining three shoulder washers over the studs, mating them with the
grommets and the blower motor mounting bracket.
e. Place the three flat washers over the studs and against the shoulder washers.
f. Carefully screw the locking nuts back onto the studs, after all of the washers are in
place.
g. Tighten the locking nuts until the grommets are flat. Loosen each locking nut by one
complete turn.
h. Attach the ground wire to the grounding terminal on the back oven wall. Secure it
with the locking nut.
i. Connect the blower motor electrical plug to J7.
11. Reassemble the remaining components on the back of the GC in the reverse order in
which they were removed.
12. Reinstall the blower fan onto the shaft.
a. The fan should be even with the beveled edge on the motor shaft. The motor shaft
should not extend more than 3 mm beyond the blower fan.
b. Secure the fan to the motor shaft using the M4 Allen wrench. Be sure that the
setscrew is in place on the flat side of the blower motor shaft.
13. Replace the oven heater baffle in the reverse order in which it was removed.
a. Guide the heater and temperature sensor wires through the pipes provided on the
oven back wall.
b. Reconnect the temperature sensor and heater wires to the relevant connector J4, J23,
J24, and J25 on the backplane board.
c. Plug the pipes with the insulation material.
d. Secure the oven heater baffle to the oven wall with the four Torx screws in the tabs.
The tab adjustment screws should be loose. Carefully center the oven heater baffle
inside of the oven. Be sure that the center hole in the oven heater baffle screen is
centered over the blower motor shaft. The tolerance is very small. The oven heater
baffle will not move left or right, nor up or down more than a couple of millimeters.
However, be sure that it is centered in the oven over the blower wheel. This will
ensure that whenever the GC is turned on, the blower wheel will not be touching the
heater element mounted to the oven heater baffle.
14. Reinstall the back cover.
a. Reconnect the ground wire to the back cover terminal.
b. Replace the cover proceeding in the reverse order in which it was removed.
15. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.

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 5 GC Main Frame Advanced Maintenance
Replacing the Flap Motor

16. Power on the GC.

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
17. Close the front door of the GC. Be sure that the oven blower is running.
a. Verify that the heater heats, and the fan rotates without contacting the heater
element.
18. Open the front door of the GC.
19. Reinstall the column.
20. Set the normal injector, detector and GC working conditions.

Replacing the Flap Motor

To replace the oven flap motor, you must remove the complete motor flap and cooling duct
assembly. The flap motor plugs into the motherboard on the GC. Therefore, you must
remove the right side panel of the GC.

 To replace the flap motor

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Remove the back cover.
a. Using a T20 Torxhead screwdriver, remove the four screws that secure the back cover
to the GC. See Figure 213.

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5 GC Main Frame Advanced Maintenance
Replacing the Flap Motor

Figure 213. Back Cover Removal

Back Cover Fixing Screws

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

6. Remove the AI/AS autosampler if present.

7. Remove the right panel.
a. Open the front door of the GC.
b. Using a T20 Torxhead screwdriver remove the screw located on the upper right
corner of the frontal frame.
c. Slide the panel towards the back of the instrument. Tilt the top of the right panel
outwards.
d. Continue to slide the panel towards the back of the GC and lift it off.
8. Remove the cooling duct. See Figure 214.

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Replacing the Flap Motor

Figure 214. Cooling Duct Removal

Cooling Duct
Fixing Screws

Flap Motor

a. Locate the three screws that secure the cooling duct to the oven wall. Remove these
screws.
b. Remove the cooling duct from the back of the GC.
9. Remove the flap motor.
a. Disconnect the oven flap motor electrical plug from J10 (Flap Motor) on the
backplane board.
b. Loosen the M4 Allen screw the secure the flap motor shaft to the railing that attaches
to the flaps.
c. Remove the two fixing screws that secure the flap motor to its support plate. See
Figure 215.

Figure 215. Flap Motor Removal (1)

M4 Allen Screw Fixing Screws

d. Remove the flap motor paying attention to not rotate it.

10. Reinstall the new motor.
11. Align the flat side of the motor shaft with the Allen screw.
12. Reassemble the flap motor and cooling duct assembly in the reverse order in which it was
removed.

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5 GC Main Frame Advanced Maintenance
Replacing the Flap Motor

13. Reconnect the power plug to connector J10 on the backplane board.
14. Reinstall the back cover.
a. Reconnect the ground wire to the back cover terminal.
b. Replace the cover proceeding in the reverse order in which it was removed.
15. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
16. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
17. Set the normal injector, detector and GC working conditions.

228 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 6

Injectors Advanced Maintenance

This chapter describes TRACE 1300/TRACE 1310 injector modules that do not require
routine maintenance, but troubleshooting may indicate they need to be cleaned or replaced.

Contents
• Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and
PTVBKF Injectors
• Removing/Replacing an Injector Module
• Cleaning the SSL Injector Body
• Cleaning the SSLBKF Injector Body
• Cleaning the HeS-S/SL Injector Body
• Cleaning the PTV Injector Head Assembly
• Cleaning the PTVBKF Injector Head Assembly

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6 Injectors Advanced Maintenance
Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF Injectors

Baking-out Contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and

PTVBKF Injectors
Perform this operation to bake-out contaminants from SSL, SSLBKF, HeS-S/SL, PTV, and
PTVBKF injectors.

 To bake-out contaminants from the injector

1. Put the inlet into Split injection mode.

2. Select Constant Flow mode and enter the normal operating Column flow value.
3. Set Split flow to 200 mL/min.
4. Purge the column with carrier flow for at least 10 minutes before heating the oven.
5. If the column is connected to the detector, set the detector 25 °C above normal operating
temperature. If the column is not attached to the detector, cap the detector fitting.
In case of PDD detector, it is strongly recommended disconnecting the column from the
detector.
6. Set the injector temperature to 300 °C or 25 °C above the normal operating temperature
to bake out contaminants from the injector, mostly through the split vent.
7. Set the oven temperature 25 °C above the GC method final oven temperature to bake
contaminants from the column. Do not exceed the column manufacturer’s maximum
temperature limit.
8. Bake-out for 30 minutes or until the detector baseline is free of contamination peaks.

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 6 Injectors Advanced Maintenance
Removing/Replacing an Injector Module

Removing/Replacing an Injector Module

This section provides the instructions for removing/replacing a front/back injector module.

Figure 216. Example of Injector Module

Captive Fixing Screws

Module Flap Cover

Lifting Handle

Figure 217. Replacing a Front Injector Module

Example of Injector Module

Figure 218. Replacing a Back Injector Module

Example of Injector Module

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6 Injectors Advanced Maintenance
Removing/Replacing an Injector Module

 To remove/replace a front/back injector module

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector on the back of the GC, and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Close the gas supplies.
6. Remove the column end from the injector.
a. Open the front door of the GC.
b. Loosen the retaining nut from the injector fitting on the upper interior wall of the
GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector.
7. Put the autosampler away if present.
a. If an AI/AS autosampler is present, move the sampler support to the right.
b. If a TriPlus or a TriPlus RSH autosampler is present, move the turret/head until
enough free space is created around the module.
8. Remove the injector module.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Keeping the module flap cover open, lift up the module from its seat in the injector
housing.
d. Place the module on a clean surface.

WARNING Make sure the o-ring is placed into its seat on the gas
connection plate.

Do not install the module if the o-ring is missing.

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 6 Injectors Advanced Maintenance
Removing/Replacing an Injector Module

9. Replace the injector module.

a. Open the module flap cover.
b. Keeping the module flap cover open, place it in its seat. Be sure to insert the 25-pin
male connector, on the bottom of the module, into the 25-pin female connector on
the injector seat of the injector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.

CAUTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

e. Keep the plug connected to the bottom.
10. Open the gas supplies.
11. Check the gas supply for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check each fitting for leaks.
b. If you detect a leak, tighten the connection, and retest it.
c. Repeat this process until all connections are leak free.
12. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
13. Power on the GC.
a. Plug the power cable to the AC Input connector on the back of the GC, and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
14. Pressurize the module with the carrier gas.
15. Check the module gas connections for leaks.
16. Remove the plug from the bottom.
17. Reconnect the column end to the injector, and verify the connection point.
18. Close the front door of the GC.
19. If present, move the autosampler towards the module to restore the original alignment.

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6 Injectors Advanced Maintenance
Cleaning the SSL Injector Body

Cleaning the SSL Injector Body

Perform this operation when a more efficient cleaning of the injector body is desired or
required due to contaminants.

 To clean the injector body

Materials needed
Ultrasonic cleaning bath
Methanol/acetone mixture 1:1
GC-grade methanol
T20 Torxhead screwdriver
Forceps or tweezers

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector. See Figure 219.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the liner. See Figure 219.
a. Use tweezers to remove the liner with the liner seal from the injector.
8. Remove the bottom parts of the injector. See Figure 219.
a. Unscrew the capillary column retaining nut, then remove the analytical column with
its ferrule from the bottom of the injector.
b. Unscrew the retaining nut with the washer and the base seal.

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 6 Injectors Advanced Maintenance
Cleaning the SSL Injector Body

Figure 219. SSL Injector Components

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal

Liner

Base Seal
Washer
Bottom Fittings
Retaining Nut

Ferrule

Capillary Column Retaining Nut Terminal Fitting for Capillary Column

9. Remove the body head o-rings. See Figure 220.

a. Using tweezers, remove both the internal and external body head o-rings.
b. Place and keep all the removed components on a clean surface.
10. Remove the injector body. See Figure 220.
a. Using a T20 Torxhead screwdriver, undo the two injector body fixing screws, and
extract the injector body from its housing.

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6 Injectors Advanced Maintenance
Cleaning the SSL Injector Body

Note Do not remove the carrier, split and purge lines o-rings.

Figure 220. SSL Injector Body Cleaning

Body Head Internal O-ring

Body Head External O-ring

SSL Injector Body Fixing Screws

SSL Injector Body

Carrier Line O-ring

Purge Line O-ring

Split Line O-ring

11. Clean the injector body.

a. Put the injector body into an ultrasonic cleaner, filled with a methanol/acetone
mixture (1:1).
b. Sonicate the injector body for about half an hour.
c. Using tweezers, remove the injector body from the bath, and dry it with an inert gas.
12. Reinstall the injector body.
a. Reinstall and fix the injector body into its housing by screwing the two fixing screws.
b. Using tweezers, replace both the internal and external body head o-rings.

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 6 Injectors Advanced Maintenance
Cleaning the SSL Injector Body

13. Reinstall the bottom parts of the injector.

a. Reinstall the retaining nut with the washer and the base seal.
b. Reinstall the analytical column.
14. Reinstall the liner
a. Using tweezers, place the liner seal over the liner, insert the liner into the injector, and
push it gently towards the bottom of the injector.
15. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector.
Fix them by screwing the ring nut.
b. Screw and tighten the injector cap to finger tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

16. Close the module flap cover.

17. If present, move the autosampler towards the module to restore the original alignment.
18. Turn the carrier gas on.
19. Set the normal oven and injector working conditions.

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6 Injectors Advanced Maintenance
Cleaning the SSLBKF Injector Body

Cleaning the SSLBKF Injector Body

Perform this operation when a more efficient cleaning of the injector body is desired or
required, due to contaminants.

 To clean the injector body

Materials needed
Ultrasonic cleaning bath
Methanol/acetone mixture 1:1
GC-grade methanol
1/8-inch -wrench
T20 Torxhead screwdriver
Forceps or tweezers

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the top parts of the injector. See Figure 221.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the liner. See Figure 221.
a. Use tweezers to remove the liner with the liner seal from the injector.
8. Remove the bottom parts of the injector. See Figure 221.
a. Unscrew the capillary column retaining nut, then remove the analytical column with
its ferrule from the bottom of the injector.
b. Unscrew the retaining nut with the washer and the base seal.

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 6 Injectors Advanced Maintenance
Cleaning the SSLBKF Injector Body

Figure 221. SSLBKF Injector Components

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal

Liner

Base Seal
Washer

Retaining Nut
Bottom Fittings
Ferrule

Capillary Column Retaining Nut

9. Remove the body head o-rings. See Figure 222.

a. Using tweezers, remove both internal and external body head o-rings.
b. Place and keep all the removed components on a clean surface.
10. Remove the injector body. See Figure 222.
a. Using a T20 Torxhead screwdriver, undo the two injector body fixing screws, and
extract the injector body from its housing.

Note Do not remove the carrier, split, and purge lines’ o-rings.

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6 Injectors Advanced Maintenance
Cleaning the SSLBKF Injector Body

Figure 222. SSLBKF Injector Body Cleaning

Body Head Internal O-ring

Body Head External O-ring

SSL Injector Body Fixing Screws

SSL Injector Body

Carrier Line O-ring

Purge Line O-ring

Split Line O-ring

11. Clean the injector body.

a. Put the injector body into an ultrasonic cleaner filled with a methanol/acetone
mixture (1:1).
b. Sonicate the injector body for about half an hour.
c. Using tweezers, remove the injector body from the bath, and dry it with an inert gas.
12. Reinstall the injector body.
a. Reinstall and fix the injector body into its housing by screwing the two fixing screws.
b. Using tweezers, replace both the internal and external body head o-rings.
13. Reinstall the bottom parts of the injector.
a. Reinstall the retaining nut with the washer and the base seal.

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 6 Injectors Advanced Maintenance
Cleaning the SSLBKF Injector Body

b. Reinstall the analytical column.

14. Reinstall the liner
a. Place the liner seal over the liner, then, Using tweezers, insert the liner into the
injector and push it gently towards the bottom of the injector.
15. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector.
Fix them by screwing the ring nut.
b. Screw and tighten the injector cap to fingertight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

16. Close the module flap cover.

17. If present, move the autosampler towards the module to restore the original alignment.
18. Turn the carrier gas on.
19. Set the normal oven and injector working conditions.

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6 Injectors Advanced Maintenance
Cleaning the HeS-S/SL Injector Body

Cleaning the HeS-S/SL Injector Body

Over time, contamination of the Instant Connect Helium Saver Injector Module will occur
due to the deposition of cored septum particles or other material not captured by the glass
wool of the injection port liner. In this case, the injector insert should be removed and cleaned
according to the following procedure.

 To clean the Instant Connect Helium Saver Injector Module injector body

Materials needed
Ultrasonic cleaning bath
Methanol/acetone mixture 1:1
GC-grade methanol
Methylene chloride
Hexane
T20 Torxhead screwdriver
Forceps or tweezers

1. Put the GC in standby condition.

2. Cool the GC oven, injector, transfer line, and ion source.
Note By pressing the Maintenance button, the GC cool down is automatically
carried out.

3. Vent the mass spectrometer and set the inlet flow rate (nitrogen) to Off. Keep the helium
enabled and pressurized as usual.
4. Put the autosampler away if present.
5. Open the module flap covers.
6. Remove the top parts of the injector. See Figure 223.
a. Unscrew the septum cap of the injector.
b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
7. Remove the liner. See Figure 223.
a. Use tweezers to remove the liner with the liner seal from the injector.
8. Remove the analytical column.
a. Unscrew the capillary column retaining nut, then remove the analytical column with
its ferrule from the bottom of the injector.

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Cleaning the HeS-S/SL Injector Body

Figure 223. Instant Connect Helium Saver Injector Module: Cleaning the Injector Body (1)

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal
Liner

9. Disconnect the helium transfer tube See Figure 224.

a. Loosen the captive screw of the helium transfer tube and remove the tube from the
gas delivery block.

Figure 224. Instant Connect Helium Saver Injector Module: Cleaning the Injector Body (2)

Gas Delivery Block

Helium Transfer Tube

10. Remove the body head o-rings. See Figure 225.

a. Using tweezers, remove both the internal and external body head o-rings.

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6 Injectors Advanced Maintenance
Cleaning the HeS-S/SL Injector Body

b. Place and keep all the removed components on a clean surface.

11. Remove the injector body. See Figure 225.
a. Using a T20 Torxhead screwdriver, undo the two injector body fixing screws, and
extract the injector body from its housing.

Note Do not remove the carrier, split and purge lines o-rings.

Figure 225. Instant Connect Helium Saver Injector Module: Cleaning the Injector Body (3)

Body Head Internal O-ring

Body Head External O-ring

Helium Transfer Tube

Helium Saver SSL Injector Body

Purge Line O-ring

Split Line O-ring
Carrier Line O-ring

12. Clean the injector body.

a. Ultrasonically clean the injector insert using a warm 1% Liquinox™ solution (or
equivalent).
b. Thoroughly rinse the insert then ultrasonically clean in chromatographic grade
methanol or acetone followed by solvents of lower polarity such as methylene
chloride and hexane. Limit ultra-sonication to 5 min in each solvent.
c. Blow dry the insert using high purity gas (do not use compressed house air as it
contains residual oils from the compressor) then assemble in the reverse order of
removal.

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Cleaning the HeS-S/SL Injector Body

Note Do not use abrasives, cleaning wires, or brushes on the inlet insert as these will
damage the passivation treatment layers. Be especially careful not to bend the short
segment of tubing at the base of the insert interior. Nothing should be inserted into
the bore of the insert other than glass liners.

13. Reinstall the injector body.

a. Reinstall and fix the injector body into its housing by screwing the two fixing screws.
b. Using tweezers, replace both the internal and external body head o-rings.
14. Reconnect the additional helium carrier gas line mating block.
15. Reinstall the liner.
a. Using tweezers, place the liner seal over the liner, insert the liner into the injector, and
push it gently towards the bottom of the injector.
16. Reinstall the analytical column. Refer to the TRACE 1300/1310 GC Hardware Manual.
17. Reinstall the top parts of the injector.
a. Place the septum holder/liner cap with the septum on the body head of the injector.
Fix them by screwing the ring nut.
b. Screw and tighten the injector cap to finger tight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

18. Close the module flap covers.

19. If present, move the autosampler towards the module to restore the original alignment.
20. Wait two or three minutes for helium to purge the inlet.
21. Turn the inlet flow back on and enable the inlet heater.
22. Set the normal injector, detector, and GC working conditions.

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6 Injectors Advanced Maintenance
Cleaning the PTV Injector Head Assembly

Cleaning the PTV Injector Head Assembly

Perform this operation when cleaning the injector head assembly is desired or required, due to
contaminants.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To clean the injector head assembly

Materials needed
Ultrasonic cleaning bath
GC-grade Methanol/acetone mixture 1:1
1/8-inch -wrench
5.5 x 25 mm Slotted Stubby Driver
T10 Torxhead screwdriver
Forceps or tweezers

1. Put the GC in standby condition.

2. Cool the oven, injectors, and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Pull down on the power switch (breaker), located at the back of the instrument, to
the position O.
b. Unplug the power cable from the AC Input connector on the back of the GC, and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Close the gas supplies.
6. Put the autosampler away if present.
7. Remove the top parts of the injector. See Figure 226.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the top of the injector head assembly.
8. Remove the liner. See Figure 226.
a. Using the slotted stubby driver provided, unscrew and remove the liner cap.

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Cleaning the PTV Injector Head Assembly

b. Use tweezers to remove the liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it. Glass
splinters might fall into the lower part of the vaporization chamber. If the glass
liner breaks, follow the instructions for “Replacing the PTV Broken Liner” on
page 123.

Figure 226. PTV Top Parts and Liner Removal

Septum Cap

Septum

Liner Cap

Liner Seal (O-ring)

Liner

Injector Head Assembly

9. Remove the fan assembly.

The fan is fixed on a support. Do not remove the fan from its support but remove the
assembly proceeding as follows:
a. Using tweezers and a T10 Torxhead screwdriver, unscrew the left fixing screw of the
support, then unscrew the right fixing screw. See Figure 227.

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Cleaning the PTV Injector Head Assembly

Figure 227. PTV Module: Fan Assembly

Unscrew and remove this screw

Fan

Fan Support

Loosen this screw

b. Carefully turn the fan assembly slightly to access the slot on the right base of the
support from the right fixing screw. See Figure 228.

Figure 228. PTV Module: Fan Assembly Removal (1)

Slot

c. Carefully extract the fan assembly from its seat, and place it sideways to the module.
Be sure to not damage the two wires. See Figure 229.

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Cleaning the PTV Injector Head Assembly

Figure 229. PTV Module: Fan Assembly Removal (2)

10. Remove the injector head.

a. Using the T10 Torxhead screwdriver, unscrew the two fixing screws, and the captive
screw that respectively fix the top and the bottom of the injector head assembly. See
Figure 230.

Figure 230. PTV Injector Head Assembly

Top Injector
Head Assembly
Fixing Screws

Bottom Injector
Head Assembly
Captive Screw

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Cleaning the PTV Injector Head Assembly

b. Holding the top of the injector head assembly with your thumb, index, and middle
fingers, turn the assembly clockwise. See Figure 231.

Figure 231. PTV Injector Head Assembly Removal (1)

Injector Head Assembly

c. Extract the assembly sideways from its housing. See Figure 232.
Note Do not remove the carrier, split, and purge lines’ o-rings on the plate.

Figure 232. PTV Injector Head Assembly Removal (2)

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Cleaning the PTV Injector Head Assembly

11. Clean the injector head.

a. Put the injector head assembly into an ultrasonic cleaner filled with a
methanol/acetone mixture (1:1).
b. Sonicate the injector head assembly for about half an hour.
c. Using tweezers, remove the injector head assembly from the bath, and dry it with
compressed clean air.
12. Reinstall the injector head.
a. Carefully reinstall the injector head assembly into its housing proceeding in the
reverse order in which it was removed.
b. Screw the two fixing screws and the captive screw fixing the top and the bottom of
the injector head assembly respectively.
13. Remount the fan assembly.
a. Carefully reinsert the fan assembly in its seat into the module.
b. Move the assembly for inserting the slot on the right base of the support slightly
under the head of the right fixing screw, and for aligning the hole on the left base
with the corresponding fixing hole.
c. Using tweezers, reinsert the left fixing screw previously removed.
d. Using a T10 Torxhead screwdriver tighten both the left and right fixing screws.
14. Reinsert the liner.
a. Place the liner seal over the liner.
b. Using tweezers, insert the liner into the injector, and push it gently towards the
bottom of the injector.
c. Reinsert and screw the liner cap using the slotted stubby driver.
15. Reinstall the upper parts of the injector.
a. Place the septum into the injector head assembly.

CAUTION We suggest replacing the septum with a new one.

b. Screw and tighten the septum cap to fingertight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

16. Close the module flap cover.

17. If present, move the autosampler towards the module to restore the original alignment.

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Cleaning the PTV Injector Head Assembly

18. Open the gas supplies.

19. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
20. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
21. Set the normal oven, injectors, and detectors working conditions.

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 6 Injectors Advanced Maintenance
Cleaning the PTVBKF Injector Head Assembly

Cleaning the PTVBKF Injector Head Assembly

Perform this operation when cleaning the injector head assembly is desired or required, due to
contaminants.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Field Service Engineers.

 To clean the injector head assembly

Materials needed
Ultrasonic cleaning bath
GC-grade Methanol/acetone mixture 1:1
1/8-inch -wrench
5.5 x 25 mm Slotted Stubby Driver
T10 Torxhead screwdriver
Forceps or tweezers

1. Put the GC in standby condition.

2. Cool the oven, injectors, and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
5. Close the gas supplies.
6. Put the autosampler away if present.
7. Open the module flap cover.
8. Remove the top parts of the injector. See Figure 233.
a. Unscrew the septum cap of the injector.
b. Using tweezers, remove the septum from the top of the injector head assembly.
9. Remove the liner. See Figure 233.

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Cleaning the PTVBKF Injector Head Assembly

a. Using the slotted stubby driver provided, unscrew and remove the liner cap.
b. Use tweezers to remove the liner with the liner seal (o-ring) from the injector.
CAUTION Be careful not to break the glass liner when removing it. Glass splinters
might fall into the lower part of the vaporization chamber. If the glass liner
breaks, follow the instructions for “Replacing the PTVBKF Broken Liner” on
page 133.

Figure 233. PTVBKF Top Parts and Liner Removal

Septum Cap

Septum

Liner Cap

Liner Seal (O-ring)

Liner

Injector Head Assembly

10. Remove the fan assembly.

The fan is fixed on a support. Do not remove the fan from its support, but remove the
assembly by proceeding as follows:
a. Using tweezers, and a T10 Torxhead screwdriver, unscrew and remove the left fixing
screw of the support, then unscrew and remove the right fixing screw. See Figure 234.

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Cleaning the PTVBKF Injector Head Assembly

Figure 234. PTVBKF Module: Fan Assembly

Unscrew and remove this screw

Fan

Fan Support

Loosen this screw

b. Carefully turn the fan assembly slightly to access the slot on the right base of the
support from the right fixing screw. See Figure 235.

Figure 235. PTVBKF Module: Fan Assembly Removal (1)

Slot

c. Carefully extract the fan assembly from its seat, and place it sideways to the module.
Be sure to not damage the two wires. See Figure 236.

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Cleaning the PTVBKF Injector Head Assembly

Figure 236. PTVBKF Module: Fan Assembly Removal (2)

11. Remove and clean the injector head.

a. Using the T10 Torxhead screwdriver, unscrew the two fixing screws, and the captive
screw that respectively fix the top and the bottom of the injector head assembly. See
Figure 237.

Figure 237. PTVBKF Injector Head Assembly

Top Injector
Head Assembly
Fixing Screws

Bottom Injector
Head Assembly
Captive Screw

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Cleaning the PTVBKF Injector Head Assembly

b. Holding the top of the injector head assembly with your thumb, index, and middle
=

fingers, turn the assembly clockwise. See Figure 238.

Figure 238. PTV Injector Head Assembly Removal (1)

Injector Head Assembly

c. Extract the assembly sideways from its housing. See Figure 239.
Note Do not remove the carrier, split, and purge lines’ o-rings on the plate.

Figure 239. PTVBKF Injector Head Assembly Removal (2)

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Cleaning the PTVBKF Injector Head Assembly

12. Clean the injector head.

a. Put the injector head assembly into an ultrasonic cleaner filled with a
methanol/acetone mixture (1:1).
b. Sonicate the injector head assembly for about half an hour.
c. Using tweezers, remove the injector head assembly from the bath and dry it with
compressed clean air.
13. Reinstall the injector head.
a. Carefully reinstall the injector head assembly into its housing proceeding in the
reverse order in which it was removed.
b. Screw the two fixing screws and the captive screw fixing the top, and the bottom of
the injector head assembly respectively.
14. Remount the fan assembly.
a. Carefully reinsert the fan assembly in its seat into the module.
b. Move the assembly slightly to position the slot on the right base of the support under
the head of the right fixing screw, and for aligning the hole on the left base with the
corresponding fixing hole.
c. Using tweezers, reinsert the left fixing screw previously removed.
d. Using a T10 Torxhead screwdriver, tighten both the left and right fixing screws.
15. Reinsert the liner.
a. Place the liner seal over the liner.
b. Using tweezers, insert the liner into the injector and push it gently towards the
bottom of the injector.
c. Reinsert and screw the liner cap using the slotted stubby driver.
16. Reinstall the upper parts of the injector.
a. Place the septum into the injector head assembly.

CAUTION We suggest replacing the septum with a new one.

b. Screw and tighten the septum cap to fingertight.

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

17. Close the module flap cover.

18. If present, move the autosampler towards the module to restore the original alignment.

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Cleaning the PTVBKF Injector Head Assembly

19. Open the gas supplies.

20. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
21. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
22. Set the normal oven, injectors, and detectors working conditions.

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Cleaning the PTVBKF Injector Head Assembly

260 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 7

Detectors Advanced Maintenance

This chapter describes TRACE 1300/TRACE 1310 detector modules that do not require
routine maintenance, but troubleshooting may indicate they need to be cleaned or replaced.

Contents
• Removing/Replacing a Detector Module
• Measuring the FID Gas Flows
• Cleaning or Replacing the FID Jet
• Measuring the NPD Gas Flows
• Measuring the FPD Gas Flows
• Cleaning or Replacing the NPD Jet
• Cleaning or Replacing the FPD Mirror Metal Plug
• Cleaning or Replacing the FPD Filter-side Heat Shields
• Cleaning or Replacing the FPD Flame-side Heat Shields
• Replacing the FPD Photomultiplier Tube

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Removing/Replacing a Detector Module

Removing/Replacing a Detector Module

This section provides the instruction for removing/replacing a front/back detector module.

Figure 240. Example of Detector Module

Captive Fixing Screws

Module Flap Cover

Lifting Handle

Figure 241. Replacing a Front Detector Module

Example of Detector Module

Figure 242. Replacing a Back Detector Module

Example of Detector Module

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 7 Detectors Advanced Maintenance
Removing/Replacing a Detector Module

 To remove/replace a front/back detector module

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.
3. Close the gas supplies.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector on the back of the GC, and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the column from the detector.
a. Open the front door of the GC.
b. Loosen the retaining nut from the detector fitting on the upper interior wall of the
GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
detector.
7. Remove the detector module.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Keeping the module flap cover open, lift up the module from its seat in the injector
housing.
d. Place the module on a clean surface.

WARNING Make sure all the four o-rings are placed into their seats on
the gas connection.

Do not install the module if the o-rings are missing.

8. Replace the detector module.

a. Open the module flap cover.

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Removing/Replacing a Detector Module

b. Keeping the module flap cover open, place it in its seat. Be sure to insert the 25-pin
male connector, on the bottom of the module, into the 25-pin female connector on
the injector seat of the injector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.
.

CAUTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

9. Remove the plug, and reconnect the column end to the detector.
10. Open the gas supplies.
11. Check the gas supply for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check each fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.
c. Repeat this process until all connections are leak free.
12. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
13. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
14. Check the module gas connections for leaks.
15. Close the front door of the GC.

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 7 Detectors Advanced Maintenance
Measuring the FID Gas Flows

Measuring the FID Gas Flows

Use this procedure for manually measuring the FID fuel and make-up gases.

 To manually measure the FID flow rates

Materials needed
Forceps or tweezers
T10 Torxhead screwdriver
Measuring Tool - Flowmeter Adapter
Electronic flowmeter (Thermo Scientific GFM Pro Flowmeter, or equivalent)

1. Put the GC in standby condition.

2. Turn the flame off. The fuel gases, hydrogen, and air, are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC, and
from the wall outlet.
6. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet
7. Open the module flap cover.
8. Disconnect the signal, glow-plug, and polarizing cables from their contacts on the cell top
cover. See Figure 243 and Figure 244.

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Measuring the FID Gas Flows

Figure 243. FID Cables

Signal Cable Straight Plug Crimp Connector

Socket Set Screw

Glow-Plug Cable Terminal body

Polarizing Cable Straight Jack Connector

Figure 244. FID Cables Disconnection

Collecting Electrode Bulkhead Jack

Glow-plug Dowel Pin

Polarizing Electrode Bulkhead Jack

a. Loosen the glow-plug cable socket set screw using a T10 Torxhead screwdriver.
Carefully pull out the terminal body of the glow-plug cable from the dowel pin on
the glow-plug element.
b. Unscrew and pull out the straight plug crimp connector of the signal cable from the
collecting electrode bulkhead jack.
c. Pull out the quick coupling straight jack connector of the polarizing cable from the
polarizing electrode bulkhead jack.
d. Carefully move the cables in order to have free space for handling the detector.
9. Remove the top parts of the detector. See Figure 245.

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Measuring the FID Gas Flows

Figure 245. FID Top Parts Removal

Detector Cap

Cell Top Cover

Insulator Ring

Collecting Electrode

Collector Insulator

a. Unscrew and remove the detector cap, paying attention to not rotate the cell top
cover.
b. Remove the cell top cover and put it in a safe place. Do not damage the FID
collecting electrode pin.
10. Remove the electrode. See Figure 245.
a. Using forceps or tweezers, carefully extract the collecting electrode, the insulator ring
and the collector insulator through the top of the detector cell. Place all the parts
removed on a clean surface.

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Measuring the FID Gas Flows

WARNING The collector insulator must be stored somewhere it can stay clean.

b. Loosen the polarizing electrode fixing screw using a T10 Torxhead screwdriver, and
extract the polarizing electrode from its seat.
c. Place the polarizing electrode on a clean surface.
11. Insert the FID gases measuring tool. See Figure 246.
a. Insert the measuring tool into the detector body.
b. Push the measuring tool downwards until it stops.

Figure 246. FID Gases Measuring Tool

Male Hose Adapter

Measuring Tool

c. Screw the detector cap onto the measuring tool. See Figure 247.

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 7 Detectors Advanced Maintenance
Measuring the FID Gas Flows

Figure 247. FID Gases Measuring Tool Assembling

Detector Cap

d. Place the flowmeter tubing over the male hose adapter of the measuring tool to begin
measuring flows. See Figure 248.

Figure 248. Measuring Tool to Flowmeter Connection

To Flowmeter

12. Power on the GC.

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Measure the gas flow rate.
a. Open the GC user interface.
b. Turn the Hydrogen flow On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.

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Measuring the FID Gas Flows

c. Turn the hydrogen flow Off.

d. Turn the Air supply On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
e. Turn the Air flow Off.
f. Turn the Makeup gas flow On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
g. Turn the Makeup Off.
14. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC, and
from the wall outlet.
15. Unscrew the detector cap, then remove the measuring tool from the detector body.
16. Reinstall the electrodes.
a. Reinsert the polarizing electrode into its seat pushing while screwing it, then screw
the fixing screw.

b. Remount the collector insulator, the collecting electrode, and its insulator ring.
17. Remount the top parts of the detector.
a. Remount the cell top cover.
b. Screw the detector cap paying attention to not rotate the cell top cover.
c. Reconnect the signal, glow-plug, and polarizing cables.
18. Close the module flap cover.
19. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
20. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
21. Turn the make-up gas on.
22. Set the normal oven and detector working conditions.
23. Ignite the flame. The fuel gases, hydrogen and air, are automatically opened.

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 7 Detectors Advanced Maintenance
Cleaning or Replacing the FID Jet

Cleaning or Replacing the FID Jet

It is good practice to clean the jet semiannually when you are analyzing particularly dirty
compounds such that the flame does not burnt properly. In case of actual damage, the jet
must be replaced.

 To clean or replace the FID ceramic jet

Materials needed
GC-grade methanol
Distilled water
Screwdriver?
Forceps or tweezers
T10 Torxhead screwdriver
Elbowed box wrench, 8-mm
Ceramic jet (if necessary)

1. Put the GC in standby condition.

2. Turn the flame off. The fuel gases, hydrogen, and air, are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC, and
from the wall outlet.
c. If external modules are present, unplug the power cable from the AC Input connector
of each external module, and from the wall outlet.
6. Open the module flap cover.
7. Disconnect the signal, glow-plug, and polarizing cables from their contacts on the cell top
cover. See Figure 249 and Figure 250.

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Cleaning or Replacing the FID Jet

Figure 249. FID Cables

Signal Cable Straight Plug Crimp

Connector

Socket Set Screw

Glow-Plug Cable Terminal body

Polarizing Cable Straight Jack Connector

Figure 250. FID Cables Disconnection

Collecting Electrode Bulkhead Jack

Glow-plug Dowel Pin

Polarizing Electrode Bulkhead Jack

a. Loosen the glow-plug cable socket set screw using a T10 Torxhead screwdriver.
Carefully pull out the terminal body of the glow-plug cable from the dowel pin on
the glow-plug element.
b. Unscrew and pull out the coaxial straight plug crimp connector of the signal cable
from the collecting electrode bulkhead jack.
c. Pull out the quick coupling coaxial straight jack connector of the polarizing cable
from the polarizing electrode bulkhead jack.
d. Carefully move the cables in order to have free space for handling the detector.
8. Remove the top parts of the detector. See Figure 251.

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Cleaning or Replacing the FID Jet

Figure 251. FID Top Parts Removal

Detector Cap

Cell Top Cover

Insulator Ring

Collecting Electrode

Collector Insulator

a. Unscrew and remove the detector cap paying attention to not rotate the cell top
cover.
b. Remove the cell top cover and put it in a safe place. Do not damage the FID
collecting electrode pin.
9. Remove the electrode. See Figure 251.
a. Using forceps or tweezers, carefully extract the collecting electrode, the insulator ring,
and the collector insulator through the top of the detector cell. Place all the parts
removed on a clean surface.

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Cleaning or Replacing the FID Jet

WARNING The collector insulator must be stored somewhere it can stay clean.

b. Loosen the polarizing electrode fixing screw using a T10 Torxhead screwdriver, and
extract the polarizing electrode from its seat. See Figure 252.

Figure 252. FID Jet (2)

Jet

Polarizing Electrode
Fixing Screw

Polarizing Electrode

c. Place the polarizing electrode on a clean surface.

10. Remove, clean, and reinstall the jet.
a. Use the elbowed box wrench for unscrewing and extracting the jet through the
detector body. See Figure 253.

Figure 253. FID Jet (2)

Jet

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 7 Detectors Advanced Maintenance
Cleaning or Replacing the FID Jet

b. Place the jet in the ultrasonic cleaner, filled with liquid detergent.
c. Sonicate the jet for about five minutes.
d. Handling the jet with forceps or tweezers. Rinse the jet using distilled water, then
methanol.
e. Place the jet on a paper towel, and let the jet air dry.

Note If after the cleaning physical condition of the jet does not permit its reuse,
replace the jet with a new one.

f. Using the proper tool, reinsert and screw the cleaned (or a new) jet into its seat in the
detector body.
11. Reinstall the electrodes.
a. Reinsert the polarizing electrode into its seat pushing while screwing it, then screw
the fixing screw.

b. Remount the collector insulator, the collecting electrode, and its insulator ring.
12. Remount the top parts of the detector.
a. Remount the cell top cover.
b. Screw the detector cap paying attention to not rotate the cell top cover.
c. Reconnect signal, glow-plug, and polarizing cables.
13. Close the module flap cover.
14. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
15. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
16. Turn the make-up gas on.
17. Set the normal detector working conditions.
18. Ignite the flame. The fuel gases, hydrogen. and air, are automatically opened.

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Measuring the NPD Gas Flows

Measuring the NPD Gas Flows

Use this procedure for manually measuring the NPD fuel and make-up gases.

 To manually measure the NPD flow rates

Materials needed
Forceps or tweezers
T6 Torxhead key
T10 Torxhead screwdriver
6 mm wrench
Measuring Tool Flowmeter Adapter
Electronic flowmeter (Thermo Scientific GFM Pro Flowmeter, or equivalent)

1. Put the GC in standby condition.

2. Switch off the thermionic source. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
6. Unplug the power cable from the AC input connector of the NPD Thermionic Source
Power Module and from the wall outlet.
If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
7. Unplug the signal cable from the detector module. See Figure 254.

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Measuring the NPD Gas Flows

Figure 254. NPD Signal Cable Removal

8. Open the module flap cover.

9. Remove the thermionic source assembly cable. See Figure 255.

Figure 255. NPD Thermionic Source Cable Removal

Thermionic Source Assembly

Thermionic Source Assembly Cable

a. Twist the ring to disconnect the thermionic source assembly cable.

b. Push and twist the lock so that the button slides up in the groove, then pull the cable
ends apart.
10. Unscrew and pull out the straight jack connector of the collecting electrode cable from
the collecting electrode bulkhead jack. See Figure 256.

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Measuring the NPD Gas Flows

Figure 256. NPD Collecting Electrode Cable Removal

Collecting Electrode Cable

Collecting Electrode Straight Jack

Connector

11. Remove the thermionic source

CAUTION The thermionic source is delicate. Be careful not to break or crack the source.
When performing maintenance on the NPD, avoid touching the source with your fingers,
and prevent it from coming in contact with other surfaces.
Wear clean, lint-free gloves to prevent contamination of parts with dirt and skin oils.

a. Using a T6 Torxhead key, loosen the dowel which fix the thermionic source
connector to the thermionic source support. See Figure 257.

Figure 257. NPD Thermionic Source Removal (1)

Thermionic Source Connector

Thermionic Source Support

Dowel

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b. Using the T10 Torxhead screwdriver, remove the three T10 Torx screws from the
thermionic source assembly. See Figure 258.

Figure 258. NPD Thermionic Source Removal (2)

T10 Torx Screw

c. Gently lift up the thermionic source connector guiding the flexible cable from the
thermionic source support, then remove the thermionic source assemble from the
NPD body. Avoid bumping the bead on the sides of the collector. See Figure 259.

Figure 259. NPD Thermionic Source Removal (3)

Thermionic Source Connector

Thermionic Source Flexible Cable

Thermionic Source Assemble

Thermionic Source Support

NPD Body
Collecting Electrode Pin

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Measuring the NPD Gas Flows

12. Remove the collecting electrode and the insulator. See Figure 260.
a. Using a 6-mm wrench, unscrew and remove the collecting electrode pin from its seat
on the detector body.
b. Using forceps or tweezers, extract the collecting electrode through the top of the
detector body.
c. Using forceps or tweezers, extract the insulator through the top of the detector body.
Place the insulator on a clean surface.

Figure 260. NPD Insulator and Collecting Electrode Removal

Collector Electrode

Insulator

WARNING The collector insulator must be stored somewhere it can stay clean.

13. Insert the NPD gases measuring tool. See Figure 261.
Figure 261. NPD Gases Measuring Tool (1)

Male Hose Adapter

Measuring Tool

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a. Insert the flowmeter adapter measuring tool into the detector body.
b. Push the flowmeter adapter measuring tool downwards until it stops.
c. Rotate the measuring tool to align the fixing holes, then insert the three screws used
to fix the thermionic source.
d. Fix the flowmeter adapter measuring tool to the detector body using a T10 Torxhead
screwdriver.
e. Place the flowmeter tubing over the male hose adapter of the measuring tool to begin
measuring flows.See Figure 262.

Figure 262. NPD Gases Measuring Tool (2)

To Flowmeter

14. Measure the gas flow rate.

a. Open the GC user interface.
b. Turn the Hydrogen flow On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
c. Turn the hydrogen flow Off.
d. Turn the Air supply On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
e. Turn the Air flow Off.
f. Turn the Makeup gas flow On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
g. Turn the Makeup Off.
15. Remove the flowmeter adapter measuring tool from the detector body proceeding in the
reverse order in which it was installed.

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a. Using forceps or tweezers, reinstall the insulator into the detector body.
16. Reinstall the insulator and the collecting electrode.
a. Using forceps or tweezers, reinstall the insulator into the detector body.
b. Place the collecting electrode in its housing.
c. Using a 6-mm wrench, screw the collecting electrode pin into its seat on the detector
body.
d. Screw the straight plug crimp connector of the collecting electrode cable to the
collecting electrode bulkhead jack.
17. Reinstall the thermionic source.
a. Remove the protective cap covering the new thermionic source.
b. Mount and guide the new source assembly on the NPD body proceeding in the
reverse order in which it was removed. Be careful not to bump the bead on the sides
of the body and collecting electrode.
c. Rotate and align the thermionic source connector, then tighten the dowel to fix the
connector to the support using the T6 Torxhead key. See Figure 263.
Figure 263. Reinstall Thermionic Source (1)

Correct Alignment

Dowel

18. Reconnect and screw the straight jack connector of the collecting electrode cable to the
collecting electrode bulkhead jack. See Figure 264.

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Figure 264. Reinstall Thermionic Source (2)

Collecting Electrode Cable

Collecting Electrode Straight Jack Connector

19. Reconnect the source assembly cable to the NPD cable and twist the ring to lock the
connection. See Figure 265.

Figure 265. Reinstall Thermionic Source (3)

20. Close the module flap cover.

21. Plug in the signal cable into its contact on the detector module. See Figure 266.

Figure 266. Plug NPD Signal Cable

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Measuring the NPD Gas Flows

22. Plug the power cable to the AC input connector of the NPD Thermionic Source Power
Module and to the wall outlet. The LED marked On blinks green.
If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
23. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
24. Turn the make-up gas on.
25. Heat the detector the working temperature.
26. Switch on the thermionic source. Hydrogen and air are automatically opened.
The LED marked On of the NPD Thermionic Source Power Module becomes solid
green.

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Cleaning or Replacing the NPD Jet

Cleaning or Replacing the NPD Jet

You should cleaning the jet semiannually when you analyze particularly dirty compounds that
the flame does not burnt properly. In case of breakage or cracking, the jet must be replaced.

 To clean or replace the NPD jet

Materials needed
Ultrasonic cleaner
Liquid detergent
GC-grade methanol
Distilled water
Forceps or tweezers
T6 Torxhead key
T10 Torxhead screwdriver
6 mm wrench
Elbowed box wrench, 8-mm
Jet (if necessary)

1. Put the GC in standby condition.

2. Switch off the thermionic source. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.
4. Turn the make-up gas off.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

5. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
6. Unplug the power cable from the AC input connector of the NPD Thermionic Source
Power Module and from the wall outlet.
If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
7. If present, move the autosampler away from the module.
8. Unplug the signal cable from the detector module. See Figure 267.

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Figure 267. NPD Signal Cable Removal

9. Open the module flap cover.

10. Remove the thermionic source assembly cable. See Figure 268.
Figure 268. NPD Thermionic Source Cable Removal

Thermionic Source Assembly

Thermionic Source Assembly Cable

a. Twist the ring to disconnect the thermionic source assembly cable.

b. Push and twist the lock so that the button slides up in the groove, then pull the cable
ends apart.
11. Unscrew and pull out the straight jack connector of the collecting electrode cable from
the collecting electrode bulkhead jack. See Figure 269.

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Figure 269. NPD Collecting Electrode Cable Removal

Collecting Electrode Cable

Collecting Electrode Straight Jack

Connector

12. Remove the thermionic source

CAUTION The thermionic source is delicate. Be careful not to break or crack the source.
When performing maintenance on the NPD, avoid touching the source with your fingers,
and prevent it from coming in contact with other surfaces.
Wear clean, lint-free gloves to prevent contamination of parts with dirt and skin oils.

a. Using a T6 Torxhead key, loosen the dowel which fixes the thermionic source
connector to the thermionic source support. See Figure 270.
Figure 270. NPD Thermionic Source Removal (1)

Thermionic Source Connector

Thermionic Source Support

Dowel

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b. Using the T10 Torxhead screwdriver, remove the three T10 Torx screws from the
thermionic source assembly. See Figure 271.

Figure 271. NPD Thermionic Source Removal (2)

T10 Torx Screw

c. Gently lift up the thermionic source connector guiding the flexible cable from the
thermionic source support, then remove the thermionic source assembly from the
NPD body. Avoid bumping the bead on the sides of the collector. See Figure 272.

Figure 272. NPD Thermionic Source Removal (3)

Thermionic Source Connector

Thermionic Source Flexible Cable

Thermionic Source Assemble

Thermionic Source Support

NPD Body
Collecting Electrode Pin

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13. Remove the collecting electrode. See Figure 273.

a. Using a 6-mm wrench, unscrew and remove the collecting electrode pin from its seat
on the detector body.
b. Using forceps or tweezers, extract the collecting electrode through the top of the
detector body.

Figure 273. NPD Collecting Electrode Removal

Collector Electrode

14. Remove and clean the jet. See Figure 274.

a. Using forceps or tweezers, extract the insulator through the top of the detector body.
Place the insulator on a clean surface.

WARNING The collector insulator must be stored somewhere it can stay clean.

b. Use the elbowed box wrench for unscrewing and extracting the jet through the
detector body.
Figure 274. Jet Removal

Insulator

Jet

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c. Place the jet in the ultrasonic cleaner, filled with liquid detergent.
d. Sonicate the jet for about five minutes.
Note If after cleaning the physical condition of the jet does not permit its reuse,
replace the jet with a new one.

15. Reinsert the jet

a. Using the proper tool, reinsert the cleaned (or a new) jet, into the detector body and
screw it in its seat.
b. Using forceps or tweezers, reinstall the insulator into the detector body.
16. Reinstall the collecting electrode.
a. Place the cleaned (or a new) collecting electrode in its housing.
b. Using a 6-mm wrench, screw the collecting electrode pin into its seat on the detector
body.
c. Screw the straight plug crimp connector of the collecting electrode cable to the
collecting electrode bulkhead jack.
17. Reinstall the thermionic source.
a. Remove the protective cap covering the new thermionic source.
b. Mount and guide the new source assembly on the NPD body proceeding in the
reverse order in which it was removed. Be careful not to bump the bead on the sides
of the body and collecting electrode.
c. Rotate and align the thermionic source connector, then tighten the dowel to fix the
connector to the support using the T6 Torxhead key. See Figure 275.

Figure 275. Reinstall Thermionic Source (1)

Correct Alignment

Dowel

18. Reconnect and screw the straight jack connector of the collecting electrode cable to the
collecting electrode bulkhead jack. See Figure 276.

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Figure 276. Reinstall Thermionic Source (2)

Collecting Electrode Cable

Collecting Electrode Straight Jack Connector

19. Reconnect the source assemble cable to the NPD cable and twist the ring to lock the
connection. See Figure 277.

Figure 277. Reinstall Thermionic Source (3)

20. Close the module flap cover.

21. Plug in the signal cable into its contact on the detector module. See Figure 278.

Figure 278. Plug NPD Signal Cable

22. If present, move the autosampler towards the module to restore the original alignment.

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23. Plug the power cable to the AC input connector of the NPD Thermionic Source Power
Module and to the wall outlet. The LED marked On blinks green.
If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
24. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
25. Turn the make-up gas on.
26. Heat the detector the working temperature.
27. Switch on the thermionic source. Hydrogen and air are automatically opened. The LED
marked On becomes solid green.

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 7 Detectors Advanced Maintenance
Measuring the FPD Gas Flows

Measuring the FPD Gas Flows

Use this procedure for manually measuring the FPD fuel gases.

 To manually measure the FPD flow rates

Materials needed
Forceps or tweezers
T10 Torxhead screwdriver
Measuring Tool Flowmeter Adapter
Electronic flowmeter (Thermo Scientific GFM Pro Flowmeter, or equivalent)

1. Put the GC in standby condition.

2. Turn the flame off. The fuel gases, hydrogen, and air, are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Remove the column end from the bottom of the detector, then plug the bottom of the
detector using the blind cap.
5. Insert the FPD gases measuring tool. See Figure 279.
a. Remove the chimney cap.
b. Place the measuring tool on the chimney.

Figure 279. FPD Gases Measuring Tool

Chimney Cap

Chimney
Measuring Tool

Male Hose Adapter

Plug

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Measuring the FPD Gas Flows

Note For measuring the flows use the male hose adapter. The plug must be used
instead the male hole adapter when a leak test is required.

6. Place the flowmeter tubing over the male hose adapter of the measuring tool to begin
measuring flows.
7. Measure the gas flow rate.
a. Open the GC user interface.
b. Turn the Hydrogen flow On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
c. Turn the hydrogen flow Off.
d. Turn the Air supply On.
i. Measure the gas flow and verify that the actual flow rate corresponds to the
setpoint value.
e. Turn the Air flow Off.
8. Remove the flowmeter tubing from the male hose adapter of the measuring tool.
9. Remove the measuring tool from the chimney.
10. Replace the chimney cap over the chimney.
11. Remove the blind cap from the bottom of the detector.
12. Reconnect the column end into the bottom of the detector.
13. Ignite the flame. The fuel gases, hydrogen and air, are automatically opened.

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Cleaning or Replacing the FPD Mirror Metal Plug

Cleaning or Replacing the FPD Mirror Metal Plug

Before cleaning or replacing the FPD mirror metal plug, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed
FPD fixing tools
Paper towels
Methylene chloride or GC-grade methanol
1-mm Allen wrench

 To clean or replace the FPD mirror metal plug

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detector from the base.
a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 280.

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Figure 280. FPD Cables Disconnection

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 281.

Figure 281. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

Note Do not lose the aluminium ring inserted between the detector head and
the base body.

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7. Remove the mirror plug.

a. Loosen the Allen screws on the mirror plug and remove it. See Figure 282.

Figure 282. FPD Mirror Plug Removal

8. Clean the mirror surface of the plug.

a. Using a clean paper towel, clean the mirror surface of the plug. If necessary, use a
solvent as methylene chloride or methanol to remove deposits, and a metal polishing
paste to restore it to the previous reflectivity.
9. Remount the mirror plug.

Note Before reinserting the mirror plug, inspect the graphite seal inside the plug’s
housing. If the seal is damaged and could not ensure tightness, replace it with a new
one.

a. Insert the cleaned or a new mirror plug in the detector body and fix it with the
relevant Allen screws. See Figure 283.

Figure 283. FPD Mirror Plug Remounting

10. Reinstall the FPD detector on the base.

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a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut. See Figure 284.

Figure 284. FPD Detector Reinstallation

FPD Fixing Nut

Aluminium Ring

b. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 285.

Figure 285. FPD Cables Connection

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11. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Set the normal working conditions.

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Cleaning or Replacing the FPD Filter-side Heat Shields

Cleaning or Replacing the FPD Filter-side Heat Shields

Before cleaning or replacing the FPD filter-side heat shield, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed
FPD fixing tools
Paper towels
FPD maintenance kit
Methylene chloride or GC-grade methanol
1-mm Allen wrench
Screwdriver

 To clean or replace the FPD filter-side heat shield

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detector from the base.
a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 286.

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Figure 286. FPD Cables Disconnection

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 287.
Figure 287. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

Note Do not lose the aluminium ring inserted between the detector head and
the base body.

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Cleaning or Replacing the FPD Filter-side Heat Shields

7. Remove the photomultiplier assembly and the filter.

a. Loosen the knurled nut that fixes the photomultiplier assembly and remove it from
the detector body. See Figure 288.
Figure 288. Photomultiplier Assembly Removal

Knurled Nut

CAUTION The photomultiplier tube could damage if exposed to ambient light with the
excitation voltage On. Make sure the power supply has been switched off before
disconnecting the tube from the detector body.

b. Remove the interferential filter from its housing, handling it very gently. Keep it
using a clean paper towel. See Figure 289.

Figure 289. Filter Removal

CAUTION Filters are fragile. Pay attention not to let the filter fall down and damage.

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Cleaning or Replacing the FPD Filter-side Heat Shields

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first remove the spacer, and then the filter.

Interferential Filter

Spacer

8. Remove the filter-side heat shield.

a. Loosen the three Allen screws that fix the filter support to the flange of the detector
body and remove it. See Figure 290.

Figure 290. Filter-side Heat Shield Removal (1)

Flange

Filter Support
b. Turn down slowly the detector body and let the filter-side heat shield come out from
the flange. Pay attention not to lose the o-ring inserted in the filter support.
Figure 291. Filter-side Heat Shield Removal (2)

Filter-Side Heat Shield

Viton® O-Ring

9. Insert a new filter-side heat shield.

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Cleaning or Replacing the FPD Filter-side Heat Shields

a. Insert a new filter-side heat shield into its housing inside the filter support.
See Figure 292.

Figure 292. Filter-side Heat Shield Replacement (1)

b. Insert the filter support into the flange and fix it tightening the three Allen screws.
Check the o-ring that fixes the heat shield is in place before tightening the screws.
See Figure 293.

Figure 293. Filter-side Heat Shield Replacement (2)

10. Remount the filter and the photomultiplier assembly.

a. Reinsert the filter into the support. The mirror face must be oriented towards the
flame. See Figure 294.
Figure 294. Filter Remounting

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CAUTION Avoid touching the filter with your fingers. If you see fingertips on the filter,
clean it using a clean paper towel and, if needed, GC-grade methanol before remounting.

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first insert the filter, and then the spacer.

Interferential Filter

Spacer

b. Reassembly the photomultiplier assembly and the detector body, then fix them
together tightening the knurled nut. See Figure 295.

Figure 295. Photomultiplier Assembly Remounting

11. Reinstall the FPD detector on the base.

a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut. See Figure 296.

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Figure 296. FPD Detector Reinstallation

b. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 297.
Figure 297. FPD Cables Connection

12. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.

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13. Power on the GC.

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
14. Set the normal working conditions.

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Cleaning or Replacing the FPD Flame-side Heat Shields

Cleaning or Replacing the FPD Flame-side Heat Shields

Before cleaning or replacing the FPD flame-side heat shield, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION When handling organic solvents you must take precautions to avoid health
hazards.

Materials needed
FPD fixing tools
Paper towels
FPD maintenance kit
Methylene chloride or GC-grade methanol
1-mm Allen wrench
Screwdriver

 To clean or replace the FPD flame-side heat shield

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detector from the base.
a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 298.

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Figure 298. FPD Cable Disconnection

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 299.

Figure 299. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

Note Do not lose the aluminium ring inserted between the detector head and
the base body.

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7. Remove the photomultiplier assembly and the filter. See Figure 300.
Figure 300. Photomultiplier Assembly Removal

Knurled Nut

a. Loosen the knurled nut that fixes the photomultiplier assembly and remove it from
the detector body.

CAUTION The photomultiplier tube could damage if exposed to ambient light with the
excitation voltage On. Make sure the power supply has been switched off before
disconnecting the tube from the detector body.

b. Remove the interferential filter from its housing, handling it very gently. Keep it
using a clean paper towel. See Figure 301.

Figure 301. Filter Removal

CAUTION Filters are fragile. Pay attention not to let the filter fall down and damage.

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Cleaning or Replacing the FPD Flame-side Heat Shields

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first remove the spacer, and then the filter.

Interferential Filter

Spacer

8. Remove the filter support.

a. Loosen the three Allen screws that fix the filter support to the flange of the detector
body and remove it. See Figure 302.
Figure 302. Filter Support Removal
Flange

Filter Support

9. Remove the filter-side heat shield.

a. Loosen the three Allen screws that fix the flange to the detector body and remove it
with the spacer that should remain inserted in the flange. Now you could access the
flame-side heat shield, and the relevant graphite seal. See Figure 303.

Figure 303. Filter-side Heat Shield Removal (1)

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b. Loosen the Allen screws that fix the mirror plug and remove it. See Figure 304.

Figure 304. Mirror Plug Removal

c. Insert the handle of a screwdriver or other un-sharpened tool in the combustion

chamber and push the flame-side heat shield and its graphite seal out from its
housing. Act gently to avoid breaking of the heat shield. See Figure 305.

CAUTION While pushing out the heat shield, pay attention not to damage the ignition
coil.

Figure 305. Filter-side Heat Shield Removal (2)

d. Remove with care traces of graphite due to the breaking of the seal.
10. Insert a new filter-side heat shield. See Figure 306.

Figure 306. Filter-side Heat Shield Replacement (1)

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 7 Detectors Advanced Maintenance
Cleaning or Replacing the FPD Flame-side Heat Shields

a. Insert a new heat shield into its housing inside the detector body.
b. Insert the spacer into the flange, letting it stand out for about 5 mm.
c. Insert a new graphite seal on the spacer, pushing it slowly until it touches the flange.

d. Insert the flange into the detector body and fix it tightening the three Allen screws.
See Figure 307.

Figure 307. Filter-side Heat Shield Replacement

nt (2)

e. Using a clean paper towel, clean the mirror surface of the plug. If necessary, use a
solvent as methylene chloride or methanol to remove deposits and a metal polishing
paste to restore it to the previous reflectivity.
f. Insert the mirror plug in the detector body and fix it with the relevant Allen screws.
See Figure 308.

Note Before reinserting the plug, inspect the graphite seal inside the plug’s
housing. If the seal is damaged and could not ensure tightness, remove it and
replace with a new one.

Figure 308. Mirror Plug Reinstallation

Graphite Seal

g. Insert the filter support into the flange and fix it tightening the three Allen screws.
Check the O-ring that fixes the heat shield is in place before tightening the screws.
See Figure 309.

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Cleaning or Replacing the FPD Flame-side Heat Shields

Figure 309. Filter Support Reinstallation

11. Reassembly the filter and the photomultiplier assembly.

a. Reinsert the filter into the support. The mirror face must be oriented towards the
flame. See Figure 310.

Figure 310. Filter Remounting

CAUTION Avoid touching the filter with your fingers. If you see fingertips on the filter,
clean it using a clean paper towel and, if needed, GC-grade methanol before remounting.

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first insert the filter, and then the spacer.

Interferential Filter

Spacer

b. Reassembly the photomultiplier assembly and the detector body, then fix them
together tightening the knurled nut. See Figure 311.

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 7 Detectors Advanced Maintenance
Cleaning or Replacing the FPD Flame-side Heat Shields

Figure 311. Photomultiplier Assembly Remounting

12. Reinstall the FPD detector on the base.

a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut. See Figure 312.

Figure 312. FPD Detector Reinstallation

b. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 313.

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7 Detectors Advanced Maintenance
Cleaning or Replacing the FPD Flame-side Heat Shields

Figure 313. FPD Cables Connection

13. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
14. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
15. Set the normal working conditions.

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 7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

Replacing the FPD Photomultiplier Tube

Before replacing the FPD photomultiplier tube, read the following precautions:

WARNING Carry out all the operations at low temperature to avoid burns. Therefore,
before beginning maintenance, cool the detector to room temperature.

CAUTION The photomultiplier tube must be replaced only when defective.

Wear clean, lint- and powder-free gloves when you handle the photomultiplier tube.

Materials needed
FPD fixing tools
FPD Photomultiplier Tube
1-mm Allen wrench
Cross head screwdriver
Gloves

 To replace the photomultiplier tube

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detector from the base.
a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 314.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 317
7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

Figure 314. FPD Cable Disconnection

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 315.

Figure 315. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

Note Do not lose the aluminium ring inserted between the detector head and
the base body.

318 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

7. Dismount the photomultiplier tube assembly.

a. Using a crosshead screwdriver unscrew the two fixing screws that fix the
photomultiplier tube housing to the photomultiplier tube holder. See Figure 316.

Figure 316. Photomultiplier Tube Housing Removal (1)

Photomultiplier Tube Holder

Photomultiplier Tube Housing

Fixing Screw
Fixing Screw

b. Remove the photomultiplier tube housing from the photomultiplier tube holder.
Holding the photomultiplier tube holder with one hand, use the other hand to pull
out the photomultiplier tube housing from the holder. During the extraction meets
some resistance due to the presence of an o-ring inside the holder. See Figure 317.

Figure 317. Photomultiplier Tube Housing Removal (2)

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 319
7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

8. Replace the defective photomultiplier tube.

Note Wear clean, lint- and powder-free gloves when you handle the
photomultiplier tube.

a. Carefully extract the photomultiplier tube from its connector. See Figure 318.

Figure 318. Photomultiplier Tube Replacement (1)

Connector

Photomultiplier Tube

b. Replace the photomultiplier tube with a new one. Make sure to correctly insert the
pins of the photomultiplier tube into the connector referring to the polarized
position. See Figure 319.

Figure 319. Connector Polarized Position

Polarized Position

320 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

9. Remount the photomultiplier tube assemble.

a. Reinsert the photomultiplier tube housing into the photomultiplier tube holder.
Holding the photomultiplier tube holder with one hand, use the other hand to
reinsert the photomultiplier tube housing into the holder. During the insertion meets
some resistance due to the presence of an o-ring inside the holder.
See Figure 320.

Figure 320. Photomultiplier Tube Housing Reinstallation (1)

b. Fix the photomultiplier tube housing to the photomultiplier tube holder.

Carefully rotate the photomultiplier tube housing up to matching its fixing holes to
the fixing holes on the photomultiplier tube holder. See Figure 321.

Figure 321. Photomultiplier Tube Housing Reinstallation (2)

c. Insert the two fixing screws into the corresponding fixing holes, then tighten the
screws using a crosshead screwdriver.

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7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

Figure 322. Photomultiplier Tube Housing Reinstallation (3)

Fixing Screw Fixing Screw

10. Reinstall the FPD detector on the base.

a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut. See Figure 323.

Figure 323. FPD Detector Reinstallation

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 7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

b. Reconnect the signal, excitation voltage and ignition/heating cables to the detector.
See Figure 324.

Figure 324. FPD Cables Connection

11. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Set the normal working conditions.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 323
7 Detectors Advanced Maintenance
Replacing the FPD Photomultiplier Tube

324 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 8

Installing Optional Kits

This chapter describes how to install the optional kits available for the TRACE 1300/TRACE
1310. See the TRACE 1300 and TRACE 1310 Spare Parts Guide for information about
ordering the upgrade kits in this chapter.

Contents
• Installing the Oven Exhaust Kit
• Installing the Merlin Microseal High Pressure Valve Kit
• Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector
• Installing the Packed Column Adapters
• Installing the HS Adapter Kit on the SSL/SSLBKF Injector
• Installing the Large Volume Splitless Kit
• Installing the Manual On/Off Valve for Single Gas Line
• Connecting a SSL/PTV Backflush System
• Connecting a SSL/PTV Backflush System for High Temperature
• Connecting a GSV Backflush System
• Installing the NoVent Microfluidics
• Installing a FTIR Make-up Module
• Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector
• Performing the Dual FPD Detector Configuration

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 325
8 Installing Optional Kits
Installing the Oven Exhaust Kit

Installing the Oven Exhaust Kit

The oven vents at the back of the GC discharge hot air up to 450 °C (842 °F) during cooling.
Installing the optional Oven Exhaust Kit (PN 19050760), the hot air from the oven vents can
be carried away to a fume hood or other exhausting devices.

The kit includes two air ducts and two 3-m length extensible aluminium tubes.
See Figure 325.

Figure 325. Air Duct and Extensible Aluminium Tube

Air Duct Extensible Aluminium Tube

 To install the oven exhaust kit

1. Put the GC in standby condition.

2. Cool the oven to room temperature.
3. Place each air duct on the oven vent inserting the hooks of the air duct into the relevant
vertical slots provided on the GC back panel.
4. Place an end of each extensible aluminium tube on each air duct.
5. Connect the other end of the extensible aluminium tube to an exhausting device.

Extensible Aluminium Tube

Air Duct
Slot

6. Set the normal GC working conditions.

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 8 Installing Optional Kits
Installing the Merlin Microseal High Pressure Valve Kit

Installing the Merlin Microseal High Pressure Valve Kit

This section provides instructions for installing the Merlin Microseal™ High Pressure Valve kit
(PN 19050735) on the SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF injectors.

Note Merlin Microseal™ is a trademark of the Merlin Instrument Company.

Introduction
The Merlin Microseal™ High Pressure Valve is a long-life replacement for the standard septum
on the SSL, SSLBKF, HeS-S/SL, PTV, and PTVBKF injectors of the TRACE 1300/TRACE
1310. High pressure capability allows operation from 15 to 700 kPa (2 - 100 psi). A top wiper
rib improves resistance to particulate contamination. The valve can be taken apart for
cleaning. High resistance to wear greatly reduces the shedding of valve particles into the
injection port liner. This eliminates a major source of septum bleed and ghost peaks in the
chromatogram. Longer life reduces the chances of valve leaks occurring during extended
automated runs. The low syringe insertion force makes manual injections easier.
The Microseal High Pressure valve requires a 0.63-mm diameter (0.025-in.) blunt tip syringe.

Syringe Compatibility — The Microseal valve should be used only with a blunt-tipped
0.63-mm diameter (0.025-in.; 23 gauge) syringe needle.
• A needle with too small a diameter, such as a 0.43-mm diameter (0.017-in.; 23 gauge)
needle, will not seal properly.
• A needle with too large a diameter will overstretch and damage the O-ring and duckbill
seals.
• A sharp-pointed or sharp-edged syringe needle will slice or pierce the seals.

Temperature and Pressure Limits — The operating temperature of the valve is lower than
the injection port temperature set point. For long term operation (> 6 months) use the
following limits for operating conditions:
• Injection port temperature < 350 °C; Pressure range: 15 - 700 kPa (2 - 100 psi)

Higher temperatures and pressures result in shorter lifetime. High temperature deterioration
can be recognized by leaks caused by stiffening and cracking of the Microseal valve,
particularly around the sealing flange.

Getting Started
Install the Microseal High Pressure Valve on the SSL, SSLBKF, HeS-S/SL, PTV, and
PTVBKF injector following the instructions in the next operating procedures.
• “To install the Merlin Microseal Valve kit on the SSL/SSLBKF and HeS-S/SL injector”
on page 328
• “To install the Merlin Microseal Valve kit on the PTV/PTVBKF injector” on page 329

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 327
8 Installing Optional Kits
Installing the Merlin Microseal High Pressure Valve Kit

 To install the Merlin Microseal Valve kit on the SSL/SSLBKF and HeS-S/SL injector

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

5. Open the module flap cover.
6. Remove the septum.

Figure 326. SSL/SSLBKF and HeS-S/SL Injector: Septum Replacement

Septum Cap

Septum

Septum Holder

a. Unscrew and remove the septum cap.

b. Using tweezers, remove the septum from the septum holder.
7. Install the Microseal valve on the injector.

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 8 Installing Optional Kits
Installing the Merlin Microseal High Pressure Valve Kit

Figure 327. SSL/SSLBKF and HeS-S/SL Injector: Microseal Valve Installation

Microseal Valve Cap

Microseal Valve

Septum Holder

a. Place the Microseal valve in the septum holder.

b. Push down gently to seat the Microseal valve onto the rim of the septum holder.
c. Screw the Microseal valve cap completely. Microseal valve overtightening and sealing
flange stressing is not mechanically allowed.
8. Close the module flap cover.
9. If present, move the autosampler towards the module to restore the original alignment.
10. Turn the carrier gas on.
11. Set the normal injector, detector, and GC working conditions.

 To install the Merlin Microseal Valve kit on the PTV/PTVBKF injector

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Put the autosampler away if present.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 329
8 Installing Optional Kits
Installing the Merlin Microseal High Pressure Valve Kit

5. Open the module flap cover.

6. Remove the septum.

Figure 328. PTV/PTVBKF Injector: Septum Replacement

Septum Cap

Septum

Injector Head Assembly

a. Unscrew the septum cap of the injector.

b. Using tweezers, remove the septum from the injector head assembly.
7. Install the Microseal valve on the injector.

330 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 8 Installing Optional Kits
Installing the Merlin Microseal High Pressure Valve Kit

Figure 329. PTV/PTVBKF Injector: Microseal Valve Installation

Microseal Valve Cap

Microseal Valve

Injector Head Assembly

a. Place the Microseal valve in the injector head assembly.

b. Push down gently to seat the Microseal valve onto the rim of the septum holder.
c. Screw the Microseal valve cap completely. Microseal valve overtightening and sealing
flange stressing is not mechanically allowed.
8. Close the module flap cover.
9. If present, move the autosampler towards the module to restore the original alignment.
10. Turn the carrier gas on.
11. Set the normal injector, detector, and GC working conditions.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 331
8 Installing Optional Kits
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector

Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector
This section provides the instruction for installing the Purge & Trap Adapter kit
(PN 19050730) on the SSL/SSLBKF injector for the use with a Purge & Trap system.
See Figure 330.

Figure 330. Purge & Trap Adapter

Purge and Trap Adapter

 To install the purge & trap adapter

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. Remove the column end from the injector.
a. Open the front door of the GC.

332 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 8 Installing Optional Kits
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector

b. Loosen the retaining nut from the injector fitting on the upper interior wall of the
GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector.
6. Put the autosampler away if present.
7. Remove the SSL/SSLBKF injector module from its seat.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Throw upward the module from its seat of the injector housing. Place the
SSL/SSLBKF module on a clean surface.

WARNING Make sure the o-ring remains into its seat on the gas connection.
Do not install the module if the o-ring is missing.

O-ring

8. Replace the module flap cover with the one’s provided. See Figure 331.

Figure 331. Module Flap Cover for Purge & Trap

Module Flap Cover

9. Reinstall the injector module into the main frame.

a. Open the module flap cover.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 333
8 Installing Optional Kits
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector

b. Place the injector module in its seat. Be sure to insert the 25-pin male connector, on
the bottom of the module, into the 25-pin female connector on the injector seat of
the injector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtighten.

CAUTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

10. Remove the top parts of the injector. See Figure 332.

Figure 332. Injector Top Parts Removal

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

a. Unscrew the septum cap of the injector.

b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
11. Replace the liner.

IMPORTANT We suggest to replace the liner currently installed into the injector with
the HS/SPME liner (PN 453A1335).

a. Use tweezer to remove the current liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it. Glass
splinters might fall into the lower part of the vaporization chamber.

b. Holding the HS/SPME liner with tweezers place a new liner seal over the liner.
c. Insert the liner into the injector and push it gently towards the bottom of the injector.

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 8 Installing Optional Kits
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector

12. Install the purge & trap adapter on the top of the injector. See Figure 333.

Figure 333. Purge & Trap Adapter (1)

Septum Cap

Ring Nut

Septum

Purge & Trap Adapter

a. Avoid touching the septum with your fingers. Insert a new septum into the septum
holder cavity of the purge & trap adapter using tweezers. See Figure 334.

Figure 334. Purge & Trap Adapter (2)

b. Place purge & trap adapter with the septum on the body head of the injector.
See Figure 335.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 335
8 Installing Optional Kits
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector

Figure 335. Purge & Trap Adapter (3)

c. Guide the ring nut on the purge & trap adapter with the septum, then fix it screwing
the ring nut. See Figure 336.

Figure 336. Purge & Trap Adapter (4)

d. Screw and tighten the septum cap to finger-tight. See Figure 337.

Figure 337. Purge & Trap Adapter (5)

336 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 8 Installing Optional Kits
Installing the Purge & Trap Adapter Kit on the SSL/SSLBKF Injector

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

13. Close the module flap cover.

14. Connect the purge & trap adapter to the Purge & Trap system. See Figure 338.

Figure 338. Purge & Trap Adapter (6)

IN

OUT

a. Using the nut and ferrule provided, connect the heated transfer line from the Purge
& Trap on the connector IN of the purge & trap adapter.
b. Using the nut and ferrule provided, connect the gas line from the injector module to
the Purge & Trap on the connector OUT of the purge & trap adapter.
15. Reconnect the column end to the injector and verify the connection point.
16. Open the gas supplies.
17. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip out the power switch (breaker) to the position I.
18. Pressurize the module with the carrier gas.
19. Check for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check the two fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.
c. Repeat this process until all connections are leak free.
20. Close the front door of the GC.
21. If present, update the autosampler for the new injection position.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 337
8 Installing Optional Kits
Installing the Packed Column Adapters

Installing the Packed Column Adapters

This section provides instructions for installing the adapters for connecting 1/8 or 1/16-in.
OD metal packed columns to the bottom of the SSL injector, FID, NPD, ECD, TCD, and
FPD. For the connection to a PDD detector see the Caution note below.

WARNING Do not try to connect packed columns if the lengths of the column ends
above the end of the ferrule are not compatible with the adapters. In this case we suggest
to connect a new packed column by using the nuts and ferrules provided with the kit.

CAUTION Connecting a packed column to a PDD detector: An adapter to connect the

PDD packed column adapter can be assembled using a piece of tube, the 1/16-in. nut and
gold plated ferrule, and a 1/8-in. to 1/16-in. reducing union in the standard outfit.

Introduction
The adapters are provided with the kit PN 19050758. The kit includes the following parts:
Table 14. Packed Column Adapters and 1/2-in. Wrench
Part Description
Adapter for the connection of the 1/8-in. OD packed column
to the SSL injector

Adapter for the connection of the 1/16-in. OD packed

column to the SSL injector

Adapter for the connection of the 1/8-in. OD packed column

to the FID, NPD, ECD, TCD, or FPD detector

Adapter for the connection of the 1/16-in. OD packed

column to the FID, NPD, ECD, or FPD detector

1/2-in. wrench

Getting Started
Install the column adapters following the instructions in the next operating procedures.
• “To install the packed column adapters” on page 339
• “To connect a new packed column to the injector and detector adapters” on page 341

338 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 8 Installing Optional Kits
Installing the Packed Column Adapters

 To install the packed column adapters

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Remove the analytical column.

a. Open the front door of the GC.
b. Loosen the retaining nut from the injector and detector fitting on the upper interior
wall of the GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector and the detector.
5. Remove the bottom parts of the SSL injector. See Figure 339.

Figure 339. SSL Injector Bottom Parts Removal

SSL Injector Bottom

Base Seal
Washer

Retaining Nut

a. Using the 1/2 wrench provided, unscrew the retaining nut with the base seal and the
washer from the bottom of the injector.

CAUTION Make sure that the liner does not come out from the bottom of the
injector.

Save the bottom parts of the SSL injector in a safe place because will be reused
when you restore the original configuration for capillary columns.

6. Install the adapter on the bottom the injector interposing the washer. See Figure 340.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 339
8 Installing Optional Kits
Installing the Packed Column Adapters

Figure 340. Adapter Installation on SSL Injector Bottom

Retaining Nut

Washer

Adapter for 1/8-in. OD Packed Column Adapter for 1/16-in. OD Packed Column

a. Finger-tighten the retaining nut of the adapter until it start to grip the bottom of the
injector.
b. Use the 1/2 wrench to firmly tighten the retaining nut of the adapter with the base
seal from the bottom of the injector.
7. Remove the bottom parts of the FID, NPD, ECD, TCD, or FPD detector.
See Figure 341.

Figure 341. Detector Bottom Parts Removal

Detector Bottom

Retaining Nut

Note Save the bottom parts of the detector in a safe place because will be reused
when you restore the original configuration for capillary columns.

a. Using the 1/2 wrench provided, unscrew the retaining nut from the bottom of the
detector.

340 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 8 Installing Optional Kits
Installing the Packed Column Adapters

8. Install the adapter on the bottom the detector interposing the washer. See Figure 342.

Figure 342. Adapter Installation on the Bottom of the Detector

Retaining Nut

Washer

Adapter for 1/8-in. OD Packed Column Adapter for 1/16-in. OD Packed Column

a. Finger-tighten the retaining nut of the adapter until it start to grip the bottom of the
detector.
b. Use the 1/2 wrench to firmly tighten the retaining nut of the adapter with the base
seal from the bottom of the injector.

 To connect a new packed column to the injector and detector adapters

Before you begin, verify that the proper adapters are installed on the injector and detector
side.
1. Preparing the metal packed column.
a. Using a 1/4-5/16-in. wrench, remove the column nut and ferrules from the base of
the injector adapter.
b. Using a 1/4-5/16-in. wrench, remove the column nut and ferrules from the base of
the detector adapter.
c. Slide the column nut and the ferrules onto the packed column injector and detector
ends in the order and direction as shown in the Figure 343 and Figure 344.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 341
8 Installing Optional Kits
Installing the Packed Column Adapters

Figure 343. Column Nut and Ferrules (1)

Ferrules

Column Nut

Adapter for 1/8-in. OD Packed Column Adapter for 1/16-in. OD Packed Column

Figure 344. Column Nut and Ferrules (2)

Ferrules

Column Nut

Adapter for 1/8-in. OD Packed Column Adapter for 1/16-in. OD Packed Column

2. Connect the packed column to the injector.

a. Insert the inlet end of the column as far as possible into bottom of the adapter.
b. Slide the ferrule up to adapter base then finger-tighten the column retaining nut until
it starts to grip the column.
c. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
3. Close the front door of the GC.
4. Turn on the gas supplies and the injector temperature.
5. Setup the GC.
a. Set the oven and injector temperature to 50 °C.
b. Allow the column to purge for few minutes.
6. Condition the column.
The column must be conditioned before inserting it into the detector.

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 8 Installing Optional Kits
Installing the Packed Column Adapters

Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the column manufacturer’s instructions.
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.

CAUTION When performing column conditioning, the column should be connected only
to the injector leaving the column outlet disconnected to avoid the possibility of
contamination of the detector.

Do not use hydrogen as the carrier for conditioning! It could vent into the oven and
present an explosion hazard.

a. Run the temperature program that is recommended by the manufacturer.

INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating

temperature.

7. Connect the column to the detector inside the GC.

a. Lower the oven temperature to 30 °C and allow it to cool.

WARNING-BURN HAZARD: The injector, detector, and oven, may be hot. Allow them to
cool to room temperature before touching them.

8. Connect the packed column to the detector.

a. Insert the inlet end of the column as far as possible into bottom of the adapter.
b. Slide the ferrule up to adapter base then finger-tighten the column retaining nut until
it starts to grip the column.
c. Use the wrench to tighten the retaining nut. Use no more pressure than is necessary
to obtain a good seal (1/4 to 1/2 turn).
9. End of the column installation.
a. Close the front door of the GC.

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8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

Installing the HS Adapter Kit on the SSL/SSLBKF Injector

This section provides the instructions for installing the HS Adapter kit PN 19050732, on the
SSL/SSLBKF injector for the use with a TriPlus 300 Headspace sampling system. See
Figure 345.

Figure 345. HS Adapter

HS adapter

 To install the HS adapter

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically car-
ried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. Remove the column end from the injector and the detector.
a. Open the front door of the GC.

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 8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

b. Loosen the retaining nut from the injector fitting on the upper interior wall of the
GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector.
6. Remove the SSL/SSLBKF injector module from its seat.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Lift the module from its seat in the injector housing. Place the SSL/SSLBKF module
on a clean surface.

WARNING Make sure the o-ring remains into its seat on the gas connection.
Do not install the module if the o-ring is missing.

O-ring

7. Replace the module flap cover with the one’s provided. See Figure 346.

Figure 346. Module Flap Cover for the HS Adapter

Module flap cover

8. Reinstall the injector module into the main frame.

a. Open the module flap cover.
b. Place the injector module in its seat. Be sure to insert the 25-pin male connector, on
the bottom of the module, into the 25-pin female connector on the injector seat of
the injector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.

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8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

CAUTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

9. Remove the top parts of the injector. See Figure 347.

Figure 347. Injector Top Parts Removal

Septum Cap

Ring nut

Septum holder/Liner cap with septum

a. Unscrew the septum cap of the injector.

b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
10. Replace the liner.

IMPORTANT We suggest replacing the liner currently installed into the injector with
the HS/SPME straight glass empty liner PN 453A1335.

a. Use tweezers to remove the current liner with the liner seal (o-ring) from the injector.

CAUTION Be careful not to break the glass liner when removing it.
Glass splinters might fall into the lower part of the vaporization chamber.

b. Holding the HS/SPME liner with tweezers, place a new liner seal over the liner.
c. Insert the liner into the injector and push it gently towards the bottom of the injector.
11. Install the HS adapter on the top of the injector. See Figure 348.

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Installing the HS Adapter Kit on the SSL/SSLBKF Injector

Figure 348. HS Adapter (1)

Septum cap

Ring nut

Septum

HS adapter

a. Avoid touching the septum with your fingers. Insert a new septum into the septum
holder cavity of the HS adapter using tweezers. See Figure 334.

Figure 349. HS Adapter (2)

b. Place the HS adapter with the septum on the body head of the injector.
See Figure 350.
Figure 350. HS Adapter (3)

c. Guide the ring nut on the HS adapter with the septum, then fix it by screwing in the
ring nut. See Figure 351.

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8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

Figure 351. HS Adapter (4)

12. Close the module flap cover.

13. Connect the carrier gas line coming from the TriPlus 300 Headspace sampling system to
the HS adapter by using the proper nut and ferrule. See Figure 352.

Figure 352. HS Adapter (5)

14. Screw and finger-tighten the septum cap provided. See Figure 353
Figure 353. HS Adapter (6)

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 8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

15. Insert the transfer line needle into the septum cap. See Figure 354.

Figure 354. Transfer Line Connection

16. Cut the piece of column end previously connected to the injector.
a. Use a scoring wafer to score and break the column in order to remove the current
ferrule and the nut.

17. Connect the column to the injector.

a. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
b. Insert the column through the injector retaining nut and the proper ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
c. Use a scoring wafer to score and break the column about 1 cm (0.4 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and the end of the
column.

d. Position the column so that the end of the column extends the proper distance of
30 mm above the end of the ferrule.
e. Insert the notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.

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8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

f. Adjust the column position so that the septum contact the bottom of the retaining
nut.
g. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
h. Remove the notched septum from the column.
18. Open the gas supplies.
19. Power on the GC.
a. Plug the power cable into the AC Input connector on the back of the GC and into
the wall outlet.
b. Flip out the power switch (breaker) to the position I.
20. Setup the GC.
a. Set the oven and injector temperature to 50 °C.
b. Use the column-flowmeter connector to verify that there is flow through the column.
If you do not have a flowmeter, dip the column outlet in a small vial of methanol.
Bubbles indicate there is flow through the column. If there is no flow, check that the
carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is
still no flow, consult the section Analytical Troubleshooting in the TRACE
1300/TRACE 1310 User Guide, or contact the Technical Support.
c. Allow the column to purge for few minutes.
21. Perform a column leak check.
a. Carefully push the capillary column end into the column section of the
column-flowmeter connector. See Figure 355.
Figure 355. Using a Flowmeter for Leak Check
A B C

b. If your GC is equipped with the touch screen as user interface, select the Leak Check
icon in the Maintenance menu, otherwise perform the Leak Check through the
Chromatography Data System by selecting the proper function.
c. Start the leak check to begin operation. The split and purge valves of the selected
channel are automatically closed and the channel is pressurized with carrier gas to the
leak check setpoint.

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 8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

d. The system monitors the pressure for one minute. If the pressure does not drop more
than the maximum allowed sensitivity value, then the leak check will pass.
If the leak check did not pass, you should use the leak detector to find and fix the
leaks.

Tip Leaks can be caused by not tightening the fitting on the column-flowmeter
connector. We recommend that you check that fitting before looking elsewhere.

e. Repeat the leak check until no leaks are indicated.

22. Calibrate the carrier gas flow (column evaluation).
a. Carefully push the capillary column end into the flow meter section of the column-
flowmeter connector. See Figure 356.

Figure 356. Column Flowmeter Connector

A B C

To flowmeter

b. Connect the flowmeter to the dedicated fitting on the column-flowmeter connector.

c. If your GC is equipped with the touch screen as user interface, select Back or Front
Column icon in the Configuration menu, otherwise perform the Column
Evaluation through the Chromatography Data System by selecting the proper
function.
d. Select the column and input the physical characteristics of the column.
e. If a pre-/post-column is present, set the length and nominal internal diameter of the
pre-/post-column in the same valid ranges for the column. The following two lines
are added to the menu.
f. According to the physical characteristics of the column, the system calculates and
displays the relevant Column K-factor.

Note For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.

g. Start column evaluation. At the end of the routine, a message will indicate that
evaluation was successful.

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8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

h. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm ID column

(1.3 – 2.0 for a 30 m, 0.25 mm ID column). If the column does not report a K-factor
within this range or within 0.1 units of the previous stored value, check for a leak or
broken column using the leak detector. The K-factor is a measured resistance for the
column. A K-factor that is too low may indicate a leak in the system, while a K-factor
that is too high may indicate a blockage.
23. Disconnect the column-flowmeter.
a. Disconnect the column from the column-flowmeter connector.
b. Remove the clear plastic component, including its fittings, from the oven and set it
aside.
c. Close the GC door.
24. Condition the column.
The column must be conditioned before inserting it into the detector.
Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the column manufacturer’s instructions.
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.

CAUTION When performing column conditioning, the column should be connected only
to the injector leaving the column outlet disconnected to avoid the possibility of
contamination of the detector.
Do not use hydrogen as the carrier for conditioning! It could vent into the oven and
present an explosion hazard.

a. Run the temperature program that is recommended by the manufacturer.

INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating

temperature.

25. Connect the column to the detector inside the GC.

a. Lower the oven temperature to 30 °C and allow it to cool.

WARNING-BURN HAZARD: The injector, detector, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.

b. Unwind the column enough to easily connect its ends to the injector and the
detector.

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 8 Installing Optional Kits
Installing the HS Adapter Kit on the SSL/SSLBKF Injector

Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.

c. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column outlet about 2.5cm (1 in.) from
the end. Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert the column through the proper detector retaining nut and ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
Tip Slide a notched septum on the column before the detector retaining nut to
make it easier to measure the proper distance between the bottom nut and end of
the column.

f. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in Table 15.
For PDD see the instruction described at the step g on page 353.
Table 15. Column Insertion Depth For FID, NPD, TCD, ECD, FPD, and PDD Detectors
FID NPD TCD ECD FPD PDD
Insert the column as far as goes and withdrawn 23 mm 125 mm 136 mm
about 2mm

i. For FID, NPD, TCD, insert the column into the detector, paying attention to
not force it further. Finger-tighten the retaining nut, then withdraw the column
2-3 mm. Tighten the retaining nut an additional a quarter turn.
ii. For ECD and FPD, insert the notched septum on the column to hold the
retaining nut in this position. Thread the retaining nut into the detector but do
not tighten.
Finger-tighten the retaining nut until it starts to grip the column plus a quarter
turn. Remove the notched septum from the column.
g. For PDD the column must penetrate 136 mm inside the capillary column adapter.

Note When inserting the capillary column into the PDD detector it might rarely
happen to feel a slight resistance. In this case, for proper column installation, pull
the column out slightly and adjust the angle before inserting it further.

IMPORTANT To install a packed column, the pre-installed capillary column adapter must
be replaced with the packed columns adapter that enters into the PDD cell for the correct
length.

26. End of the column installation.

a. Close the front door of the GC.

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8 Installing Optional Kits
Installing the Large Volume Splitless Kit

Installing the Large Volume Splitless Kit

This section provides instruction for installing the Large Volume Splitless kit (PN 19050725)
on your TRACE 1300/TRACE 1310.

The Large Volume Splitless injector is a setup of the standard splitless injector, where the
introduction of large amount of liquid samples can be performed manually, or with the
TriPlus RSH, TriPlus 100 Liquid Sampler, or AI/AS 1310 autosampler.

Large Volume-Splitless kit includes:

• Two dummy filters
• Two o-rings for dummy filters
• An deactivated connector (press-fit)
• An uncoated precolumn (5 m x 0.32 mm ID)
• A dedicated splitless liner (set of 5)
• LV-SL Assistant software

 To install the large volume splitless kit

1. Put the GC in standby condition.

2. Cool the oven and injector to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
4. Put the autosampler away if present.
5. Open the module flap cover

6. Replace the filters with the dummy filters.

a. Remove both the filters from their seats by turning them counter-clockwise.

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 8 Installing Optional Kits
Installing the Large Volume Splitless Kit

Carrier line filter

Split line filter

b. Install the dummy filters in their seats interposing the o-ring, then turn them
clockwise.

O-ring

Dummy filter

7. Replace the current liner installed into the Split/Splitless injector with the dedicated
splitless liner.
8. Close the module flap cover.
9. Install the uncoated precolumn.
a. Open the oven door.
b. Disconnect the analytical column from the bottom of the injector.

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8 Installing Optional Kits
Installing the Large Volume Splitless Kit

c. Connect an end of the precolumn to the bottom of the injector by using the proper
nut and ferrule. Position the precolumn so that its end extends a distance of 5 mm
above the end of the ferrule.
10. Couple the precolumn to the analytical capillary column.
Split/Splitless Injector

Detector

Deactivated connector

Precolumn

Analytical column

a. Properly cut the fused silica column ends pay attention to achieve a clean square cut
by using a ceramic scoring wafer or sapphire scribe.

CAUTION A poorly cut will produce an insufficient seal.

b. insert the precolumn and analytical column ends into the relevant ports of the
deactivated connector.

Note To create a good seal between all the parts, will be necessary to increase the
oven temperature up to 200 °C.

11. Close the oven door.

12. If present, move the autosampler towards the module to restore the original alignment.
13. Turn the carrier gas on.
14. Set the injector, detector, and GC working conditions.

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 8 Installing Optional Kits
Installing the Manual On/Off Valve for Single Gas Line

Installing the Manual On/Off Valve for Single Gas Line

This section provides instructions for installing the manual on/off toggle valve for single gas
line kit (PN 19050756) on a GC gas inlet of your TRACE 1300/TRACE 1310.
See Figure 357.

Figure 357. Manual Toggle Valve Installation

CLOSE

The manual toggle valve is used for manually opening and closing the gas flow coming from
the supply line.

It is particularly useful when for any reason you need to manually interrupt the flow of a gas
into the GC, for example: before the replacement of a module, to save the consumption of a
gas when its flow is unnecessary, and so on.
The kit can be installed only on a single gas line, then if you need managing more gas lines, up
to six kits can be installed accordingly.

 To install the manual on/off valve kit

Note This procedure describes the installation of a manual on/off valve on a single gas
line. In the example the installation of the valve on the Front Carrier gas line is considered.

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.
3. Turn the carrier gas off, and wait for the carrier pressure to go to zero.
4. On the back of the GC disconnect the gas supply tube and the gas inlet fitting with its
o-ring from the GC gas inlet where the manual on/off valve must be installed.

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8 Installing Optional Kits
Installing the Manual On/Off Valve for Single Gas Line

Use a 7/16-in. wrench for unscrewing the fittings.

Gas supply tube Gas inlet fitting

O-ring

5. Screw the gas inlet fitting 1/8-in. provided into the gas inlet port interposing the o-ring.
Use a 7/16-in. wrench for tightening the fitting.

Gas inlet fitting 1/8-in.

6. Using the nut and ferrules provided, connect the valve outlet to the gas inlet fitting
1/8-in. Make sure that the arrow marked on the valve body indicating the direction of the
flow is turned forward the GC gas inlet. Use a 7/16-in. wrench for tightening the fittings.

Nut and ferrules

Gas flow direction

7. Remove the nut and the ferrules provided from the valve inlet.

Remove nut and ferrules

8. Connect the gas supply tube previously disconnected (see the step 4). Use a 7/16-in.
wrench for tightening the fittings.

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 8 Installing Optional Kits
Installing the Manual On/Off Valve for Single Gas Line

Gas supply tube

9. Turn the carrier gas on.

10. Open or close the flow of the supply gas turning the black handle of the valve up or down
respectively.
11. Set the injectors, detectors, and GC working conditions.

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8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

Connecting a SSL/PTV Backflush System

This section provides instructions for connecting your SSLBKF/PTVBKF injector modules
with precolumn and analytical capillary column into the oven. Refer to the chapters SSLBKF
Injector Module and PTVBKF Injector Module in the TRACE 1300/TRACE 1310 User
Guide. This system operates up to 300 °C.

The operation consists of the following operating procedures:

• “To assemble the Tee connector on the mounting bracket” on page 360
• “To install the mounting bracket” on page 367
• “To connect backflush line, precolumn, and capillary column” on page 369
• “To install of a second backflush system” on page 373

CAUTION Before starting make sure the SSLBKF/PTVBKF injector module is correctly
installed into its seat.

 To assemble the Tee connector on the mounting bracket

Tee Connector
Retaining Nut

Graphite/Vespel Ferrule Clamp

Backflush Line Fittings Clip

Graphite/Vespel Ferrule Nut

Retaining Nut Threaded Shaft

Mounting Bracket
Top Nut Tee Connector Assembly Bottom

1. Remove the nut and the top end of the mounting bracket. This nut secures the two
sections of the mounting bracket. See Figure 358.

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 8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

Figure 358. Assembling Tee Connector and Mounting Bracket (1)

Nut
Top End

Note Wait reinstalling the top end of the mounting bracket and the nut. These parts
will be reinstalled during the installation of the mounting bracket into the GC oven.
See “To install the mounting bracket” on page 367.

2. Slide the clip and the clamp on the body of the mounting bracket. See Figure 359.

Figure 359. Assembling Tee Connector and Mounting Bracket (2)

Clamp Clip

3. Screw the part of threaded shaft of the Tee connector with the smaller diameter into the
Tee connector, then screw the nut on the threaded shaft. Use a 2.5 mm key to tighten the
shaft. See Figure 360.

Figure 360. Assembling Tee Connector and Mounting Bracket (3)

Nut
Threaded Shaft
Tee Connector

4. Screw the Tee connector into clamp. Turn the threaded shaft until it protrudes the clamp,
then turn the nut until it grip the clamp. See Figure 361.

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8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

Figure 361. Assembling Tee Connector and Mounting Bracket (4)

5. Move the Tee connector support on the lower part of the mounting bracket, then
finger-tighten the nut up to grip the clamp without overtightening. This allow to adjust
the position of the Tee connector during the installation of backflush line, precolumn,
and capillary column.
6. At this point mount the mounting bracket into the oven of the GC. See “To install the
mounting bracket” on page 367.

 To install the mounting bracket into the GC oven

Install the mounting bracket near the front of the GC oven on the right-hand side. This will
keep the mounting bracket out of the way of the column.

Proceed as follows:
1. Make sure that the nut at the top end of the mounting bracket are removed. See
Figure 358 on page 361.
2. Line up the bottom of the mounting bracket into two holes on the bottom side of the GC
oven.
3. Hold the mounting bracket directly upright, and twist the top end of it so that it is
securely attached to the GC oven holes.
4. Let out the top of the mounting bracket until it is long enough to be secured into two
holes on the bottom of the GC oven.
5. Replace the nut.
6. Tighten the nut until the mounting bracket is securely attached to the top and bottom of
the GC oven.
Figure 362 shows the result of the operation.

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 8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

Figure 362. Mounting Bracket Installed in the GC

Tee Connector

Clamp

7. At this point connect backflush line, precolumn, and capillary column to the Tee
connector. See “To connect backflush line, precolumn, and capillary column” on
page 369.

 To connect backflush line, precolumn, and capillary column

The result of this operation is schematically shown in Figure 368.

Figure 363. Connecting Backflush Line, Precolumn, and Capillary Column

Precolumn

Backflush Line

Capillary Column

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8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

Note Could be necessary to adjust the position of the Tee connector when installing
backflush line, precolumn, and column to stress as minimal as possible.

1. Connect the backflush line metal tube coming from the bottom of the SSLBKF injector
module or PTVBKF injector module to the Tee connector.
Tip The backflush line must be bent as required by the position of the Tee connector
on the mounting bracket.

a. Slide the retaining nut and the ferrule onto the end of the backflush line.
b. Insert the backflush line into the Tee connector.
c. Finger-tighten the retaining nut until it starts to grip the backflush line.
d. Use the 5 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
2. Connect the precolumn to the Tee connector.

Note Always use original Thermo Fisher Scientific precolumns.

The use of precolumns not meeting the specifications of our products does not ensure
a good operation of the instrument and may even compromise the analytical results.

Thermo Fisher Scientific precolumn (2 m; 10 m) are mounted on the appropriate

rack and identified with a relevant label fixed on the rack.

a. Place the precolumn on the column support.

b. Slide the retaining nut and the 0.8 mm Vespel/graphite ferrule onto the precolumn
with the flat end facing towards the Tee connector. Be careful to avoid damaging the
graphite ferrule when inserting the precolumn. Always use a new ferrule of the
correct diameter.
c. Cut 1 cm from the precolumn end.
d. Insert the precolumn into the Tee connector in the side with the dot that it must be
normally be turned towards the ceiling of the GC oven.
e. Finger-tighten the precolumn retaining nut until it starts to grip the precolumn.
f. Use the 1/4-in. wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
3. Connect the precolumn to the injector base.

a. Slide the retaining nut and the graphite ferrule onto the fused silica precolumn (0.53
mm ID; 2 m length) with the bevelled end facing towards the injector.
b. Cut 1 cm from the precolumn.
c. Insert the precolumn into the injector and slide the ferrule up to the injector
base.Insert the precolumn about 3 cm into the bottom of the injector.

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 8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

d. Finger-tighten the precolumn retaining nut until it starts to grip the precolumn.
e. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
4. Connect the capillary column to the Tee connector and to the precolumn.
See Figure 369.

Figure 364. Precolumn and Capillary Column Connection

Precolumn

Backflush Line

35 mm

Capillary Column

a. Place the column on the column support.

b. Slide the proper retaining nut and Vespel/graphite ferrule onto the column with the
flat end facing towards the Tee connector. Be careful to avoid damaging the ferrule
when inserting the column.
c. Cut 1 cm from the column end.
d. Use typewriter correction fluid or a felt-tipped pen to mark the position of the ferrule
bottom 35 mm from the end of the column.
e. Carefully insert the column into the precolumn up to reach the marked position.
The capillary column end must protrudes the Tee connector; if not, repeat the
operations.
f. Finger-tighten the column retaining nut until it starts to grip the column.
g. Use the 1/4-in. wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal.

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8 Installing Optional Kits
Connecting a SSL/PTV Backflush System

5. Connect the analytical column to the detector base.

a. Slide the retaining nut and the graphite ferrule onto the capillary column with the
bevelled end facing the detector base.
Be careful to avoid damaging the graphite ferrule when inserting the column.
b. Cut 2–3 cm from the column end.
c. Insert the column into the detector base body and slide the ferrule up to the detector
base.
d. Finger-tighten the column retaining nut until it starts to grip the column.
e. Push the column through the detector base according to the detector in use.
f. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.

 To install of a second backflush system

If a second SSL/PTV Backflush system is installed, you can connect both the systems by using
two Tee connectors mounted on the same mounting bracket as schematically shown in
Figure 370.

Figure 365. Schematically Connection of a Second Backflush System

Precolumn

Backflush Line

Capillary Column

Precolumn

Backflush Line

Capillary Column

Tip Turn the Tee connector as required to allow the installation of the two precolumns.

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 8 Installing Optional Kits
Connecting a SSL/PTV Backflush System for High Temperature

Connecting a SSL/PTV Backflush System for High Temperature

This section provides instructions for connecting your SSLBKF/PTVBKF injector modules
with precolumn and analytical capillary column into the oven for application at high
temperature. For the purpose, the kit PN 19050254 is required. Refer to the chapters
SSLBKF Injector Module and PTVBKF Injector Module in the TRACE 1300/TRACE 1310
User Guide. This system operates at a temperature more than 300 °C.

The operation consists of the following operating procedures:

• “To install the mounting bracket” on page 367
• “To connect backflush line, precolumn, and capillary column” on page 369
• “To install of a second backflush system” on page 373

CAUTION Before starting make sure the SSLBKF/PTVBKF injector module is correctly
installed into its seat.

 To install the mounting bracket

Install the mounting bracket near the front of the GC oven on the right-hand side. This will
keep the mounting bracket out of the way of the column.

Proceed as follows:
1. Remove the nut at the top end of the mounting bracket. This nut secures the two sections
of the mounting bracket. See Figure 366.
Figure 366. Mounting Bracket Parts
Bottom Tee Connector Nut Top

2.Push the top of the mounting bracket down.

3. Line up the bottom of the mounting bracket into two holes on the bottom side of the GC
oven.
4. Hold the mounting bracket directly upright, and twist the top end of it so that it is
securely attached to the GC oven holes.
5. Let out the top of the mounting bracket until it is long enough to be secured into two
holes on the bottom of the GC oven.
6. Replace the nut.

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Connecting a SSL/PTV Backflush System for High Temperature

7. Tighten the nut until the mounting bracket is securely attached to the top and bottom of
the GC oven.
Figure 367. Mounting Bracket Installed in the GC

Tee Connector

Clamp

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 8 Installing Optional Kits
Connecting a SSL/PTV Backflush System for High Temperature

Note If the Tee connector should not be assembled into its clamp, proceed as follows:
1. Completely screw the nut on the threaded shaft of the Tee connector.
−

Clamp

Tee Connector

Retaining Nut
Threaded Shaft

Note that the clamp has two holes: the smaller one is threaded while the bigger other
is not threaded.
−

Not Threaded Hole

Threaded Hole

2. Pay attention to insert the threaded shaft of the Tee connector into the clamp through
the bigger hole not threaded up to grip the threaded hole.

3. Turn the Tee connector until the threaded shaft begins to protrude the clamp.
−

4. Move the Tee connector support on the lower part of the mounting bracket, then
finger-tighten the nut up to grip the clamp without overtightening. This allow to
adjust the position of the Tee connector during the installation of backflush line,
precolumn, and capillary column.

 To connect backflush line, precolumn, and capillary column

The result of this operation is schematically shown in Figure 368.

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Connecting a SSL/PTV Backflush System for High Temperature

Figure 368. Connecting Backflush Line, Precolumn, and Capillary Column

Backflush Line Injector 1 = Tee Connector Mounted on the Tee holder
2 = Graphite Ferrule 0.8 mm OD
3 = Retaining Nut

Pre-column

3
2

2
3

Capillary
Column

Note Could be necessary to adjust the position of the Tee connector when installing
backflush line, precolumn, and column to stress as minimal as possible.

1. Connect the backflush line metal tube coming from the bottom of the SSLBKF injector
module or PTVBKF injector module to the Tee connector.
Tip The backflush line must be bent as required by the position of the Tee connector
on the mounting bracket.

a. Slide the retaining nut and the 1.0 mm graphite ferrule onto the end of the backflush
line with the bevelled end facing towards the Tee connector. Be careful to avoid
damaging the graphite ferrule when inserting the tube.
b. Shortly activate the backflush with carrier open to eliminate possible graphite pieces
before connecting the Tee connector.
c. Insert the backflush line into the Tee connector.
d. Finger-tighten the retaining nut until it starts to grip the backflush line.

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 8 Installing Optional Kits
Connecting a SSL/PTV Backflush System for High Temperature

e. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
2. Connect the precolumn to the Tee connector.

Note Always use original Thermo Fisher Scientific precolumns.

The use of precolumns not meeting the specifications of our products does not ensure
a good operation of the instrument and may even compromise the analytical results.

Thermo Fisher Scientific precolumn (2 m; 10 m) are mounted on the appropriate

rack and identified with a relevant label fixed on the rack.

a. Place the precolumn on the column support.

b. Slide the retaining nut and the 0.8 mm graphite ferrule onto the precolumn with the
bevelled end facing towards the Tee connector. Be careful to avoid damaging the
graphite ferrule when inserting the precolumn. Always use a new ferrule of the
correct diameter.
c. Cut 1 cm from the precolumn end.
d. Insert the precolumn into the Tee connector in the side with the dot that it must be
normally be turned towards the ceiling of the GC oven.
e. Finger-tighten the precolumn retaining nut until it starts to grip the precolumn.
f. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
3. Connect the precolumn to the injector base.

a. Slide the retaining nut and the graphite ferrule onto the fused silica precolumn (0.53
mm ID; 2 m length) with the bevelled end facing towards the injector.
b. Cut 1 cm from the precolumn.
c. Insert the precolumn into the injector and slide the ferrule up to the injector
base.Insert the precolumn about 3 cm into the bottom of the injector.
d. Finger-tighten the precolumn retaining nut until it starts to grip the precolumn.
e. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
4. Connect the capillary column to the Tee connector and to the precolumn.
See Figure 369.

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Connecting a SSL/PTV Backflush System for High Temperature

Figure 369. Precolumn and Capillary Column Connection

Pre-column

Pre-column

Backflush Line

Capillary
35 mm
Column

Capillary
Column

a. Place the column on the column support.

b. Slide the proper retaining nut and graphite ferrule onto the column with the bevelled
end facing towards the Tee connector. Be careful to avoid damaging the graphite
ferrule when inserting the column.
c. Cut 1 cm from the column end.
d. Use typewriter correction fluid or a felt-tipped pen to mark the position of the ferrule
bottom 35 mm from the end of the column.
e. Carefully insert the column into the precolumn up to reach the marked position. The
capillary column end must protrudes the Tee connector; if not, repeat the operations.
f. Finger-tighten the column retaining nut until it starts to grip the column.
g. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal.
5. Connect the analytical column to the detector base.
a. Slide the retaining nit and the graphite ferrule onto the capillary column with the
bevelled end facing the detector base.
Be careful to avoid damaging the graphite ferrule when inserting the column.

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 8 Installing Optional Kits
Connecting a GSV Backflush System

b. Cut 2–3 cm from the column end.

c. Insert the column into the detector base body and slide the ferrule up to the detector
base.
d. Finger-tighten the column retaining nut until it starts to grip the column.
e. Push the column through the detector base according to the detector in use.
f. Use the 6 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.

 To install of a second backflush system

If a second SSL/PTV Backflush system is installed, you can connect both the systems by using
two Tee connectors mounted on the same mounting bracket as schematically shown in
Figure 370.

Figure 370. Schematically Connection of a Second Backflush System

Precolumn
to Front Injector

BackFlush Line

Capillary Column
to Front Detector

Precolumn
to Back Injector

Backflush Line

Capillary Column
to Back Detector

Tip Turn the Tee connector as required to allow the installation of the two precolumns.

Connecting a GSV Backflush System

This sections provides instructions for connecting your Gas Sampling Valve module with
precolumn and analytical capillary column into the oven of the TRACE 1300/1310 GC.
Refer to the chapters Gas Sampling Valve (GSV) Module in the TRACE 1300/TRACE 1310
User Guide.
The Gas Sampling Valve backflush system is provided with the kit PN 19050764.

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Connecting a GSV Backflush System

 To connect backflush line, precolumn, and analytical capillary column

CAUTION Before starting make sure the Gas Sampling Valve (GSV) module is correctly
installed into its seat.

The result of this operation is schematically shown in the example of Figure 371.
Figure 371. Connecting GSV Backflush Line, Precolumn, and Analytical Capillary Column

GSV Backflush Line Metal Tubing

Precolumn

Three-way Connector

Analytical Capillary Column

Note Could be necessary to adjust the position of the three-way connector when installing
backflush line, precolumn, and capillary column to stress as minimal as possible.

When the GSV module is inserted into its position on the upper deck of the GC, the
backflush line metal tubing protrudes into the GC oven.
1. Open the front door of the GC.
2. Remove nut and ferrule from the metal tubing of the GSV nodule.
3. Connect the backflush line metal tubing coming from the bottom of the Gas Sampling
Valve module to the three-way connector.
a. Slide the 1/32-in. nut and the Vespel/Graphite ferrule PN 29003428 onto the end of
the backflush line with the bevelled end facing towards the three-way connector.
b. Insert the backflush line into the central position of the three-way connector.
c. Slide the retaining nut onto the backflush line through its side cut.
d. Use the 5 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
4. Connect the precolumn to the Tee connector.
a. Place the precolumn on the column support.
b. Slide the 1/32-in. nut and the proper Vespel/Graphite ferrule onto the precolumn
with the bevelled end facing towards the three-way connector. Always use a new
ferrule of the correct diameter.
c. Cut 1 cm from the precolumn end.

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 8 Installing Optional Kits
Connecting a GSV Backflush System

d. Insert the precolumn in one of the two external positions of the three-way connector.
e. Finger-tighten the precolumn retaining nut until it starts to grip the precolumn.
f. Use the 5 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
5. Connect the precolumn to the injector base.
a. Slide the M6 nut and the graphite ferrule onto the fused silica precolumn with the
bevelled end facing towards the injector.
b. Cut 1 cm from the precolumn.
c. Insert the precolumn into the injector and slide the ferrule up to the injector base.
Insert the precolumn till it reaches the mechanical stop, than drawn it back of 1 mm.
d. Finger-tighten the precolumn retaining nut until it starts to grip the precolumn.
e. Use the 1/4-in. wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal without overtighten.
6. Connect the capillary column to the three-way connector.
a. Place the capillary column on the column support.
b. Slide the 1/32-in. nut and the proper Vespel/Graphite ferrule onto the capillary
column with the bevelled end facing towards the three-way connector.
c. Cut 1 cm from the end of the capillary column.
d. Insert the end of the capillary column in the free position of the three-way connector.
e. Finger-tighten the column retaining nut until it starts to grip the column.
f. Use the 5 mm wrench to tighten the retaining nut. Use enough pressure necessary to
obtain a good seal.
7. Connect the capillary column to the detector base.
a. Slide the nut and the graphite ferrule onto the capillary column with the bevelled end
facing the detector base.
b. Be careful to avoid damaging the graphite ferrule when inserting the capillary
column.
c. Cut 2–3 cm from the end of the capillary column.
d. Insert the capillary column into the detector base body and slide the ferrule up to the
detector base.
e. Finger-tighten the capillary column retaining nut until it starts to grip the column.
f. Push the capillary column through the detector base according to the detector in use.
g. Use the 1/4-in. wrench to tighten the retaining nut.
h. Use enough pressure necessary to obtain a good seal without overtighten.

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8 Installing Optional Kits
Installing the NoVent Microfluidics

Installing the NoVent Microfluidics

This section provides instructions for installing the NoVent™ Microfluidics on your TRACE
1300 or TRACE 1310 GC, and your ISQ or TSQ 8000 Series mass spectrometer.

Contents
• “Connecting the NoVent Microfludics Module to the TRACE 1300/1310” on
page 376
• “Installing the Mounting Bracket” on page 379
• “Preparing the NoVent Microfluidics Restrictor Tubing” on page 381
• “Attaching the Ferrule and Nut to the GC Column” on page 384
• “Attaching the New Tubing to the Transfer Line” on page 385
• “Connecting the Capillaries to the Microfluidics Splitter” on page 390
• “Configuring the Post-Column” on page 391
• “Using the Module” on page 394

Connecting the NoVent Microfludics Module to the TRACE 1300/1310

According to the configuration of your TRACE 1300 or TRACE 1310 GC, a dummy
module is present in the free site where the detector module is not installed.
If no dummy module is present, and no vacant module position is present on either the
TRACE 1300 or TRACE 1310 GC or on the optional gas valve oven (if you have one), you
must take one of the existing and installed modules off in order to install the NoVent
Microfluidics.
Figure 372. Location of Detector Modules on your TRACE 1300/TRACE 1310 GC

Dummy Front Detector Module Dummy Back Detector Module

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

 To connect the NoVent Microfluidics module to the TRACE 1300/1310 GC

1. Cool the oven, injector or injectors, transfer line, ion source, and any installed GC
detectors to room temperature and shut down the GC.

WARNING - BURN HAZARD: The injectors, detectors, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.

2. Push down on the power switch to power off the GC.

3. Shut down the TSQ 8000 Series or ISQ Series mass spectrometer using the software. See
your mass spectrometer's user guide for more information. The heaters and the
turbo-molecular pump power off.
4. If you are using hydrogen as a carrier gas, unscrew the hydrogen safety screw on the front
door of the mass spectrometer.

WARNING - FIRE HAZARD: If you are using hydrogen, do NOT reach over the top of the
instrument to power it off. Instead, reach around the right side or go to the back of the
instrument and flip down the power switch.

Figure 373. Finding the Vent Valve on the Mass Spectrometer

Vacuum Interlock Shield Power Switch

Vent Valve Knob

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8 Installing Optional Kits
Installing the NoVent Microfluidics

5. Open the front door of the mass spectrometer.

6. Look behind the right side of the vacuum interlock shield and twist the vent valve knob
one and a half times in a counter-clockwise direction to open the vent valve.
7. Wait five minutes for the mass spectrometer to vent.
8. Open the front door of the GC and loosen the transfer line nut. Then pull the column
back (into the oven) about 5 cm to ensure the column is no longer in the ion source.
9. Close all GC gas supplies. Shut the carrier gas supply off at its source, such as the tank.
10. Remove the dummy module from the position where the NoVent Microfluidics will be
installed. The most convenient location for accessing the tubing connections is the front
position.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew and remove the two captive fixing
screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
detector housing. Place the dummy module on a clean surface.
d. Remove the gas plug by unscrewing its fixing screw using a T20 Torxhead
screwdriver.

Figure 374. Removing the Detector Gas Plug

Gas Block Plug on Detector Seat

O-rings

Gas Connections Without Gas Block Plug

WARNING Make sure all four o-rings are correctly seated on the gas connection. Do not
install the module if the o-rings are missing.

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

11. Install the No-Vent module.

a. Open the module flap cover.
b. Keeping the module flap cover open, place the module in its seat. Be sure to insert the
25-pin male connector, on the bottom of the module, into the 25-pin female
connector on the detector seat of the detector housing.
c. Use a T20 screwdriver to tighten the three captive fixing screws without
overtightening.
Note Tighten the center screw first and then secure the side screws.

d. Close the module flap cover.

12. Power on the GC.
13. Switch the module on so that the LED light glows green and start the carrier gas flowing
to purge the line.

Note This module requires a constant carrier gas pressure of 60 psig (410 kPa).

Installing the Mounting Bracket

Install the mounting bracket near the front of the GC oven on the right-hand side. This will
keep the mounting bracket out of the way of the column.

Note If you already have a mounting bracket installed in your GC with either the SSL
backflush or PTV backflush system, skip this step.

 To install the mounting bracket

1. Remove the nut at the top end of the mounting bracket. This nut secures the two sections
of the mounting bracket. See Figure 375.
Figure 375. Mounting Bracket Parts
Bottom Holder Nut Top

2. Push the top of the mounting bracket down.

3. Line up the bottom of the mounting bracket into two holes on the bottom side of the GC
oven.

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8 Installing Optional Kits
Installing the NoVent Microfluidics

4. Hold the mounting bracket directly upright, and twist the top end of it so that it is
securely attached to the GC oven holes.
5. Let out the top of the mounting bracket until it is long enough to be secured into two
holes on the bottom of the GC oven.
6. Loosely replace the nut, then rotate the top and bottom in opposite directions.
7. Tighten the nut until the mounting bracket is securely attached to the top and bottom of
the GC oven.
Figure 376. Mounting Bracket Installed in the GC

8. Attach the Thermo Scientific™ microfluidics splitter to the mounting bracket.

The microfluidics splitter snaps into place. The small hole should be positioned at the
top. See Figure 377 for the correct orientation.

Figure 377. Correct Orientation for the Microfluidics Splitter

Small Hole

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

9. Position the holder for the microfluidics splitter upright and use two 7 mm wrenches to
secure it. See Figure 378.

Figure 378. Securing the Holder for the Microfluidics Splitter

Preparing the NoVent Microfluidics Restrictor Tubing

If you are using helium as a carrier gas, use the 30 cm length of 75 μm fused silica tubing in
your kit (two are supplied) to connect the NoVent Microfludics module to the splitter.
If you are using hydrogen as a carrier gas, use the 80 cm length of 75 μm fused silica tubing in
your kit (one is supplied) to connect the NoVent Microfludics module to the splitter.
This tubing acts as a flow restrictor to limit the helium flow from the NoVent microfluidics
module.You must swage a SilTite™ ferrule to this tubing.
You will need the following materials to connect the tubing to new ferrule and nut. They are
all included in the complete kit.
• SilTite FingerTite™ pre-swage tool—to pre-swage the SilTite FingerTite ferrule to the GC
module
• SilFlow™ pre-swage tool and SilFlow FingerTite tool—to pre-swage the SilFlow ferrule to
the microfluidics splitter
• SilFlow nut—to connect the tubing to the microfluidics splitter
• SilTite ferrule (1)
• SilFlow ferrules (3)

Note If you are using a 0.25 mm id column, use a 0.4 mm SilFlow ferrule and a 0.4 mm
SilFlow FingerTite jig. If you are using a 0.32 mm id column, use a 0.5 mm SilFlow
ferrule and a 0.5 mm SilFlow FingerTite jig.

1. Use a scoring wafer to cut the end off the tubing.

2. Use the SilTite FingerTite pre-swage tool in your kit to pre-swage the SilTite ferrule to
your tubing. See Figure 379. The tubing should extend just past the tool when
pre-swaging the ferrule. After the ferrule is swaged, remove the tool and confirm the
ferrule does not slide on the tubing.

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8 Installing Optional Kits
Installing the NoVent Microfluidics

Then cut the tubing to about 2 mm past the tip of the ferrule. See Figure 379.

Figure 379. Pre-swaging the Ferrule to the 30 cm Fused Silica Tubing

SilTite FingerTite Pre-swage Tool

Ferrule

3. Insert the ferrule and the tubing into the NoVent microfluidics module. See Figure 380.

Figure 380. Securing the 30 cm Fused Silica Restrictor to the No-Vent Microfluidics Module

4. Add the FingerTite screw to the module and tighten.

5. Now you must prepare the other end of the tubing to connect to the microfluidics 3-port
splitter. It is much easier to attach the ferrule to the tubing while it is outside the GC.
You will connect it to the splitter later.
6. Position a SilFlow nut and SilFlow ferrule onto the tubing as shown in Figure 381.

Figure 381. Positioning the SilFlow Nut and SilFlow Ferrule Correctly on the Tubing

7. Use a scoring wafer to cut the tubing after inserting it through the ferrule. See Figure 382.
Then use the SilFlow pre-swage tool to secure the ferrule into position. When done
properly, the tubing will extend slightly past the tip of the ferrule.

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

It is important to use the SilFlow pre-swage tool in order to prevent crushing the tip of
the fused silica. See Figure 382.

Figure 382. Cutting the Tubing with the Scoring Wafer

8. Place the column and ferrule into SilFlow pre-swage tool until the tubing reaches the
bottom of the tool. When done properly, the tubing will extend slightly past the tip of the
ferrule. It is important to use the pre-swage tool in order to prevent crushing the tip of the
fused silica. See Figure 383.

Figure 383. Inserting the Column and Ferrule into the SilFlow Pre-Swage Tool

SilFlow
Pre-Swage Tool

9. Use the SilFlow FingerTite tool to swage the ferrule to the tubing. Be sure to keep the tip
of the fused silica bottomed out in the pre-swage tool. See Figure 384.

Figure 384. Swaging the Ferrule Using the SilFlow FingerTite Tool

SilFlow
FingerTite
Pre-Swage Tool

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8 Installing Optional Kits
Installing the NoVent Microfluidics

10.Remove the jig, and lay the tubing carefully on the bottom of the GC until you are ready
to connect it to the microfluidics splitter.

Attaching the Ferrule and Nut to the GC Column

You will need the following materials to connect the column to new ferrule and nut. They are
all included in the complete kit.
• SilFlow FingerTite tool
• SilFlow pre-swage tool
• SilFlow nut
• SilFlow ferrules
Note If you are using a 0.25 mm id column, use a 0.4 mm SilFlow ferrule and a 0.4 mm
SilFlow pre-swage tool. If you are using a 0.32 mm id column, use a 0.5 mm SilFlow
ferrule and a 0.5 mm SilFlow pre-swage tool.

 To add the new ferrule and nut to the column

1.Attach the ferrule to the column while it is outside the GC. You will connect it to the
module later.
2. Position the SilFlow nut and SilFlow ferrule onto the column as shown in Figure 385.

Figure 385. Positioning the Nut and Ferrule Correctly on the Column

3. Use a scoring wafer to cut the column after inserting it through the ferrule. Then use the
appropriate pre-swage tool to secure the ferrule into position. When done properly, the
tubing will extend slightly past the tip of the ferrule. It is important to use the pre-swage
tool in order to prevent crushing the tip of the fused silica. See Figure 386.

Figure 386. Cutting the Column with the Scoring Wafer

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

4. Place the column and ferrule into the SilFlow pre-swage tool until the column reaches the
bottom of the jig. See Figure 387.

Figure 387. Inserting the Column and Ferrule into the SilFlow Pre-Swage Tool

SilFlow
Pre-Swage Tool

5. Use the FingerTite tool to swage the ferrule to the column. Be sure to keep the tip of the
fused silica bottomed out in the pre-swage tool. See Figure 388.

Figure 388. Swaging the Ferrule Using the SilFlow FingerTite Tool

SilFlow FingerTite Tool

6. Remove the pre-swage tool, and lay the column carefully on the bottom of the GC until
you are ready to connect it to the microfluidics splitter.

Attaching the New Tubing to the Transfer Line

Your kit comes with a 0.6 m and 1.2 m deactivated segments of fused silica tubing having an
internal diameter of 0.17 mm. This tubing has fused ends. You must cut these ends off the
tubing and attach a SilFlow ferrule before connecting it. Use this tubing to connect the
microfluidics splitter to the transfer line.

Note Use the 60 cm tubing if you are using helium as a carrier gas. Use the 120 cm length
of tubing if you are using hydrogen as a carrier gas.

When connecting the column to the transfer line, you may use either the regular transfer line
nut or the spring loaded transfer line nut with the graphite Vespel® ferrule.

 To connect the column using the regular transfer line nut

1. Lower the oven temperature and allow it to cool.

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8 Installing Optional Kits
Installing the NoVent Microfluidics

2. Confirm that the MS is vented and remove the current transfer line nut and ferrule.
3. Unwind about one turn of the column from the column outlet end.

Note Wear clean, lint- and powder-free gloves when you handle the column and
transfer line ferrule.

4. Wipe approximately 300 mm (12 in.) of the column with a tissue soaked in methanol.
5. Choose an appropriate ferrule for the outer diameter of your column.

Note If the maximum oven temperature in your method is ≥ 290 °C (554 °F),
Thermo Fisher Scientific recommends using a spring loaded transfer line nut with a
graphite Vespel® ferrule or a SilTite™ nut and ferrule. By cycling the oven at and above
this temperature, expansion and contraction of the graphite Vespel® material can
cause leaks in the transfer line.

6. Insert the column through the transfer line nut and ferrule, entering through the tapered
end of the ferrule. Wipe the column again with a tissue soaked in methanol.

Figure 389. Transfer Line Nut and SilTite Ferrule Orientation

Flat on the Transfer Line Nut

Transfer Line Nut

SilTite Ferrule
7. Insert the column into the column measuring tool (see Figure 390), which is in the ISQ
Toolkit, so that it is even with the lines at the end of the column. Figure 391 indicates
proper positioning of the column in the tool for accurate measuring.
8. Use a scoring wafer to score and break the column. Use a magnifying glass to check for an
even, flat cut. Repeat if necessary.
9. Use a 5/16 in. wrench to hold the column measuring tool steady.

Figure 390. Column Measuring Tool

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

10. While holding the column measuring tool steady, tighten the transfer line nut with a
1/4 in. wrench until the column just stops moving in the ferrule.
11. Turn the transfer line nut 1 flat backward so the column is able to move in the ferrule
with slight resistance.
12. Line up the outlet of the column with the arrows on the end of the column measuring
tool.

Figure 391. Lining Up the Column in the Column Measuring Tool

Column Outlet

13. Place a septum with a notch cut into it behind the transfer line nut. The septum marks
the place on the column where it should exit the nut.

Figure 392. Positioning the Septum

Transfer Line
Column Nut
Measuring Tool
Septum

14. Pull the column back from the transfer line nut. Do not move the septum from its
position on the column.

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8 Installing Optional Kits
Installing the NoVent Microfluidics

Figure 393. Pulling the Column Back from the Transfer Line Nut
Column
Column
Measuring Tool

Transfer Line
Nut Septum

15. Loosen the transfer line nut from the column measuring tool.
16. Remove the column, transfer line nut and ferrule from the column measuring tool,
making sure not to move the septum from its location on the column.
17. Insert the column into the transfer line.

Figure 394. Inserting the Column into the Transfer Line

Ferrule Transfer Line
Column
Nut

Transfer Line Septum

18. Tighten the transfer line nut until it is just secure enough so that you cannot move it.
19. Loosen the nut by turning it exactly 1 flat backward.
20. Position the column in the transfer line. Use the septum as a guide to measure the correct
length you should insert the column. Be careful not to change the location of the septum
on the column.

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Installing the NoVent Microfluidics

Figure 395. Positioning the Column in the Transfer Line

Transfer Line Column

Septum

21. Tighten the nut 1 flat forward—back to where it is secure enough in the transfer line that
you cannot move it.
22. Tighten the nut 1 additional quarter turn.
23. Remove the cut septum.
24. Close the front door of the GC.
Note If you are using a SilTite ferrule, follow the instructions that come with SilTite
ferrules. If you are using a graphite Vespel ferrule, they require conditioning to ensure
a leak-tight seal. See the ISQ Spare Parts Guide for information about ordering these
ferrules.

25. Condition the graphite Vespel ferrule:

a. Raise the oven temperature to the maximum temperature you will operate the GC
column.
b. Wait 10 minutes.
c. Lower the oven temperature to 40 °C (104 °F) and allow it to cool before continuing.

WARNING BRUN HAZARD The oven may be hot. Allow it to cool to room temperature
before opening it. The injector will still be hot, so do not touch it.

d. Retighten the transfer line nut.

26. Restore working conditions.
a. Raise the oven temperature to the initial temperature that you will use.
b. Turn on vacuum compensation on the GC.
c. Twist the vent valve clockwise to close the valve. Be sure not to pin. the o-ring.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 389
 d. If you are using hydrogen as a carrier gas, replace the front panel screw.
e. Replace all remaining hydrogen safety screws if you are using hydrogen.
27. Power on the mass spectrometer.

WARNING - FIRE HAZARD: If you are using hydrogen, do NOT reach over the top of the
instrument to power it on. Instead, reach around the side or go to the back of the
instrument and flip up the power switch.

28. Once the instrument is pumped down and able to scan, click Air & Water / Tune on the
Dashboard view air water spectra and look for evidence of leaks with a large m/z 28 signal.
If you observe a leak, stop scanning and gently tighten the nut in small increments until
no leaks appear when scanning.

Connecting the Capillaries to the Microfluidics Splitter

You can attach now attach all the capillaries to the microfluidics splitter.
 To connect the capillaries to the microfluidics splitter

1. Place the ferrule connected to the original GC column into capillary B location on the
microfluidics splitter. See Figure 396. This corresponds to the bottom of the three holes
on a correctly installed splitter.

Figure 396. Correct Orientation for the Microfluidics Splitter

GC Column

Tip If a ferrule gets stuck in the microfluidics splitter, use a thumbtack or similar
pointed tool for removal. Insert the point of the tool between the ferrule and threads
and press so that the ferrule is forced off center. This will dislodge the ferrule.

2. Use the FingerTite tool to secure the nut you previously attached to the column to top of
the three holes on the microfluidics splitter.
3. Orient the capillary connected to the transfer line and the one attached to the no-vent
module as shown in Figure 397.
 8 Installing Optional Kits
Installing the NoVent Microfluidics

Figure 397. Correct Orientation of Columns to Microfluidics Splitter

Capillary Restrictor to Transfer Line

GC Column
Capillary Restrictor to No-Vent Module

4. Close the GC door.

5. Close the vent valve knob.
6. Power on the mass spectrometer.
7. Ensure the switch is set to Off on the NoVent microfluidics module.
8. Let the mass spectrometer pump down for a minimum of one hour and then check the
air-water spectrum for gross leaks. Assuming the convection gauge and ion gauge (if
present) indicate appropriate pressures, small leaks can be located by spraying with Freon,
argon, or another suitable gas near the tubing connections.

Configuring the Post-Column

After installing the NoVent microfluidics, you must enter the post-column length and ID
before running samples. This section contains instructions for configuring your post-column
settings on both a TRACE 1310 and a TRACE 1300.

 To enter post-column settings on a TRACE 1310

1. On the home page of the TRACE 1310 touchscreen, click the Configuration icon.

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Installing the NoVent Microfluidics

Figure 398. Locating the Configuration Icon on the TRACE 1310 Touchscreen
Configuration Icon

2. The default GC Configuration (Oven) screen opens. Click the Back Column or Front
Column button, corresponding to where you installed the No-vent microfluidics.
3. The GC Configuration (Front or Back Column) screen opens. Click the down arrow to
access the post column settings.
4. Select Yes from the Post Column? menu. Enter the post column length and ID in the
boxes below.

Figure 399. Configuring the Post Column Settings

Home Icon

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

IMPORTANT For hydrogen, the Post Column Length is 1.2 m. For helium, the Post
Column Length is 0.6 m. The Post Column ID is 0.17 mm for both hydrogen and
helium.

5. Click the home icon when you are finished.

 To enter the post-column settings on a TRACE 1300

1. Open Xcalibur by clicking the icon on your desktop

2. The Xcalibur Roadmap opens. Select TRACE 1300 Series GC from the list of
instruments.
3. Click Column Setup in the Status pane.

Figure 400. Xcalibur Roadmap

Status Pane

Column
Setup

4. The Column Setup window opens. Check the Using Post-Column checkbox and enter
the post-column length and ID.

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8 Installing Optional Kits
Installing the NoVent Microfluidics

Figure 401. Column Setup Options

Post-column Settings

5. Click Apply.
6. Click Close.

Using the Module

The GC does not recognize the presence of the NoVent microfluidics, so it is invisible to the
system. The module receives only the voltages for supplying the solenoid valve and the
On/Off light from the GC.
The normal condition of the module is Off. If the switch is in the Off position, the Status
LED is off. See Figure 402.

Figure 402. Module in Off Condition

Status LED Off

Switch on Off Position

Before replacing the column or performing maintenance on an injector module, flip the
switch to the On position. Ensure the mass spectrometer is in standby mode and the filament
is off. The status LED lights up as solid green indicating that the solenoid valve is activated.
The carrier gas flows into the mass spectrometer to avoid breaking vacuum. See Figure 403.

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 8 Installing Optional Kits
Installing the NoVent Microfluidics

Figure 403. Module in On Condition

Status LED On

Switch on On Position

When you have completed your tasks, flip the switch to the Off position. The status LED
turns Off indicating the solenoid valve is deactivated.

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8 Installing Optional Kits
Installing a FTIR Make-up Module

Installing a FTIR Make-up Module

This section provides instructions for installing the FTIR Make-up module on your
TRACE 1300/1310 for the connection with the Thermo Scientific Nicolet iS50 FT-IR
Spectrometer. According to the configuration of your TRACE 1300/TRACE 1310, a dummy
module is present into the free site where the detector module is not installed.
See Figure 404.

Figure 404. Add a Front/Back Detector Module

Dummy Front Detector Module Dummy Back Detector Module

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

The FTIR Make-up module must be installed into the detectors housing, and supplied
through the line of the front/back carrier gas.

IMPORTANT If the front/back carrier gas is not already used by an injector module, you
must install the front/back carrier gas inlet, and connect the carrier gas line as described in
the section “Making the Gas Supply Plumbing Connections” on page 7.

The module is equipped with an ON/OFF needle valve for the regulation of the gas flow.
• Open the needle valve manually turning the knob counter-clockwise.
• Close the needle valve manually turning the knob clockwise.

Perform the regulation of the gas flow using a little flat head screwdriver.
• Increase the gas flow turning the flow regulation screw counter-clockwise.
• Decrease the gas flow turning the flow regulation screw clockwise.

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Installing a FTIR Make-up Module

The standard operating value is in the range 0.2- 0.3 mL/min. Refer to the Nicolet iS50 FT-IR
Spectrometer Manual for details.

ATTENTION The flow of the carrier gas depends on the inlet pressure. Set the carrier gas
flow according to the working pressure.

The components of the FTIR module are shown in Figure 405.

Figure 405. FTIR Make-up Module

Open/Close Knob

Flow Regulation Screw

ON/OFF Knob:
Turn clockwise for closing
Turn counter-clockwise for opening

Flow Regulation Screw:

Turn clockwise for decreasing
Turn counter-clockwise for increasing

Needle Valve VICI-CNV1A10S1

Bottom Fitting

Nut and Ferrules

 To add a FTIR Make-up module

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.

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8 Installing Optional Kits
Installing a FTIR Make-up Module

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the dummy module from the position where the FTIR module will be installed.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew and remove the two captive fixing
screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
detector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connections by unscrewing its fixing screw
using a T20 Torxhead screwdriver. See Figure 406.

Figure 406. Detector Gas Block Plug

Gas Block Plug on Detector Seat

O-rings

Gas Connections Without Gas Block Plug

WARNING Make sure all the four o-rings are placed into their seats on the gas
connection. See Figure 406.

Do not install the module if the o-rings are missing.

7. Open the front door of the GC.

8. Plug the FTIR Make-up module into the main frame.
a. Open the module flap cover.

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Installing a FTIR Make-up Module

b. Keeping the module flap cover open, place the module in its seat.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.
.

ATTENTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

9. Connect the 1/16-in tubing provided.
a. Connect an end of the 1/16-in tubing to the bottom fitting of the module using the
Swagelock® nut and ferrules provided.
b. Connect the other end of the tubing to the transfer line of the FT-IR spectrometer.
Refer to the Nicolet iS50 FT-IR Spectrometer Manual for details.
10. Open the gas supplies.
11. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
13. Close the front door of the GC.

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8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

This section provides the instruction for installing the Hot Injection Adapter kit
(PN 19050733) on the SSL/SSLBKF injector for the use with an external device for gas
sampling. See Figure 330.

Figure 407. Hot Injection Adapter

Hot Injection Adapter

The Hot Injection Adapter consists of the following parts: head, short PTV liner, liner seal,
and stem. See Figure 408.

Figure 408. Hot Injector Adapter Assembly

Head
Liner Seal

Short PTV Liner

Stem

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 8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

 To install the hot injection adapter on the injector

1. Assemble the hot injection adapter if not already done. See Figure 409.

Figure 409. Adapter Assembly

Liner Seal

Liner

Fixing
Screws
Stem

Guides

a. Place the liner seal on the liner.

b. Insert the liner into the stem.
c. Couple the head and the stem paying attention to properly align the corresponding
holes doing match the polarizing guides on the head and on the flange of the stem.
d. Fix the stem to the head screwing the three fixing screws using a T8 Torxhead
screwdriver.

Note The stem conducts heat from the body of the SSL/SSLBKF injector to the
head of the adapter allowing a hot injection.

2. Put the GC in standby condition.

3. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Close the gas supplies.

5. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
6. Remove the column end from the injector.

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8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

a. Open the front door of the GC.

b. Loosen the retaining nut from the injector fitting on the upper interior wall of the
GC oven.
c. Remove the analytical column with its nut and ferrule from the bottom of the
injector.
7. Put the autosampler away if present.
8. Remove the SSL/SSLBKF injector module from its seat.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Throw upward the module from its seat of the injector housing. Place the
SSL/SSLBKF module on a clean surface.

WARNING Make sure the o-ring remains into its seat on the gas connection.
Do not install the module if the o-ring is missing.

O-ring

9. Replace the module flap cover with the one provided. See Figure 331.

Figure 410. Module Flap Cover for Hot Injection

Fixing Holes

Module Flap Cover

Note The fixing holes present on the top of the module flap cover can be used for
making more stable a sampling device that requires to be seat directly on the injector.

10. Reinstall the injector module into the main frame.

a. Open the module flap cover.

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Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

b. Place the injector module in its seat. Be sure to insert the 25-pin male connector, on
the bottom of the module, into the 25-pin female connector on the injector seat of
the injector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtighten.

CAUTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

11. Remove the top parts of the injector. See Figure 332.

Figure 411. Injector Top Parts Removal

Septum Cap

Ring Nut

Septum Holder/Liner Cap with Septum

Liner Seal (O-ring)

Liner

a. Unscrew the septum cap of the injector.

b. Unscrew the ring nut.
c. Remove the septum holder/liner cap with septum from the injector body head.
12. Remove the liner and its seal.

a. Use tweezer to remove the current liner with the liner seal (o-ring) from the injector.
13. Remove the injector body. See Figure 412.

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8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

Figure 412. SSL Injector Body Removal

SSL/SSLBKF Injector Body Fixing Screws

External Body Head O-ring

Internal Body Head O-ring

SSL Injector Body

Carrier Line O-ring

Purge Line O-ring

Split Line O-ring

a. Using a T20 Torxhead screwdriver, undo the two injector body fixing screws, and
extract the injector body from its housing.

Note Do not remove the carrier, split, and purge lines o-rings.

14. Install the spacer plate. See Figure 413.

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 8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

Figure 413. Spacer Plate

O-rings

Spacer Plate

a. Place the spacer plate and its three o-ring on the injector base.

Note The three o-ring of the spacer plate do not replace the carrier, purge, and
split lines o-rings but are put on them, then six o-rings are present on the base.

15. Reinstall the injector body. See Figure 414.

Figure 414. Injector Body Reinstallation

a. Reinstall and fix the injector body into its housing by screwing the two fixing screws.

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8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

b. If required, replace both the internal and external body head o-rings using tweezers.

16. Install the hot injection adapter on the top of the injector. See Figure 333.

Figure 415. Hot Injection Adapter (1)

Hot Injection Adapter

a. Avoid touching the septum with your fingers. Insert a new septum into the septum
holder cavity of the hot injection adapter using tweezers. See Figure 334.

Figure 416. Hot Injection Adapter (2)

b. Guide the ring nut on the hot injection adapter with the septum, then fix it screwing
the ring nut. See Figure 336.

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 8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

Figure 417. Hot Injection Adapter (3)

c. Screw and tighten the septum cap to finger-tight. See Figure 418.

Figure 418. Hot Injection Adapter (4)

CAUTION Do not overtighten the septum cap because you might damage the septum and
affect instrument performance.

17. Close the module flap cover.

18. Connect the hot injection adapter to the device for the sampling of gas. See the example
in Figure 419.

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8 Installing Optional Kits
Installing the Hot Injection Adapter Kit on the SSL/SSLBKF Injector

Figure 419. Hot Injection Adapter (5)

IN

OUT

Note If only a port of the adapter is connected to the device for the sampling of gas,
close the other one with the blind nut provided.

19. Reconnect the column end to the injector and verify the connection point.
20. Open the gas supplies.
21. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip out the power switch (breaker) to the position I.
22. Pressurize the module with the carrier gas.
23. Check for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check the two fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.
c. Repeat this process until all connections are leak free.
24. Close the front door of the GC.
25. If present, update the autosampler for the new injection position.

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 8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

Performing the Dual FPD Detector Configuration

This section provides the instructions for performing the Dual FPD configuration by
connecting a second photomultiplier tube with a different interferential filter on the same
detector control module. See Figure 420.

Figure 420. Dual FPD Configuration

To perform the Dual FPD configuration you need the following devices:
• Two Flame Photometric Detector modules
• A Dual FPD upgrade kit PN 19050783

IMPORTANT The Dual FPD must be installed onto the FPD control module placed into
the back site of the detectors housing.

The FPD control module placed into the front site of the detector housing must be
equipped with the PT100 simulator, provided with the kit, instead of the FPD detector,
and will supply the second photomultiplier of the configuration.

The operation consists of the following steps:

• Remotion of the front and back FPD Detectors from the relevant detector control
module.
• Disassembling the photomultiplier tube from a FPD detector.
• Assembling the second photomultiplier tube on the other FPD detector to obtain the
Dual FPD configuration.
• Remotion of the jet from the front detector control module.
• Installation of the PT100 simulator, provided with the kit, on the detector base of the
front detector control module.

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8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

Note For writing convention, we consider the disassembling of the photomultiplier tube
from the FPD detector installed on the front detector control module, and the assembling
of the Dual FPD configuration on the FPD detector installed on the back detector
control module. We name them secondary FPD detector and primary FPD detector
respectively.

 To assembly the Dual FPD configuration

Materials needed
Dual FPD Upgrade Kit PN 19050783
Gloves
FPD fixing tools
Appropriate interferential filter (394 nm for Sulphur; 526 nm for Phosphorous; 610 nm for Tin
Methylene chloride or GC-grade methanol
Screwdriver
T6 Torxhead key
T10 Torxhead screwdriver
1-mm Allen wrench
5 mm wrench
5/16-in tube wrench

Initial operations

1. Put the GC in standby condition.

2. Switch off the flame. Hydrogen and air are automatically closed.
3. Cool the detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If other external modules are present, unplug the power cable from the AC Input
connector of each module, and from the wall outlet.
6. Remove the FPD detectors from the base of the respective detector control module.

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 8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

a. Disconnect the signal, excitation voltage, and ignition/heating cables from the
detector. See Figure 421.

Figure 421. FPD Cable Disconnection

Ignition/Heating Cable

Signal Cable

Excitation
Voltage
Cable

b. Using the tool provided with the system, loosen the fixing nut on the base of the
detector and remove it. See Figure 422.

Figure 422. FPD Detector Removal

FPD Fixing Nut

Aluminium Ring

Note Do not lose the aluminium ring inserted between the detector head and
the base body.

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8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

c. Place both the FPD detectors on a clean and free workbench.

Disassembling the secondary FPD detector

7. Remove the photomultiplier tube assembly and the filter from the cell of the secondary
FPD detector. See Figure 423.

Figure 423. Photomultiplier Assembly Removal

Knurled Nut

a. Loosen the knurled nut that fixes the photomultiplier assembly and remove it from
the detector body.

CAUTION The photomultiplier tube could damage if exposed to ambient light with the
excitation voltage On. Make sure the power supply has been switched off before
disconnecting the tube from the detector body.

b. Remove the interferential filter from its housing, handling it very gently. Keep it
using a clean paper towel. See Figure 424.

Figure 424. Filter Removal

CAUTION Filters are fragile. Pay attention not to let the filter fall down and damage.

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 8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first remove the spacer, and then the filter.

Interferential Filter

Spacer

8. Remove the filter a support and the flange assembly from the cell of the secondary FPD
detector.
a. Loosen the three smaller Allen screws that fix the flange to the detector body and
remove it with the spacer that should remain inserted in the flange See Figure 425.

Figure 425. Filter Support and Flange Assembly Removal

Fixing Screws

b. Now you could access the flame-side heat shield, and the relevant graphite seal.
Loosen the Allen screws that fix the mirror plug and remove it. See Figure 426.

Figure 426. Mirror Plug Removal

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8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

c. Insert the handle of a screwdriver or other un-sharpened tool in the combustion

chamber and push the flame-side heat shield and its graphite seal out from its
housing. Act gently to avoid breaking of the heat shield. See Figure 427.

CAUTION While pushing out the heat shield, pay attention not to damage the ignition
coil.

Figure 427. Filter-side Heat Shield Removal

d. Remove with care traces of graphite due to the breaking of the seal.
e. Reinstall the mirror plug.

Assembling the primary FPD detector in Dual FPD configuration

Perform the Dual FPD configuration on the primary FPD detector using the components
removed from the secondary FPD detector.
9. Remove the mirror plug from the cell of the primary FPD detector.
a. Loosen the Allen screws that fix the mirror plug and remove it. See Figure 428.

Figure 428. Mirror Plug Removal

10. Insert the filter support and the flange assembly into the primary FPD detector.
a. Insert the heat shield into its housing inside the detector body.
b. Insert the spacer into the flange, letting it stand out for about 5 mm.

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 8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

c. Insert the graphite seal on the spacer, pushing it slowly until it touches the flange.

d. Fix the assembly tightening the three Allen screws. See Figure 429.

Figure 429. Filter Support and Flange Assembly Installation

11. Place the appropriate filter and the photomultiplier tube assembly.

a. Reinsert the filter into the support. The mirror face must be oriented towards the
flame. See Figure 430.

Figure 430. Filter Remounting

CAUTION Avoid touching the filter with your fingers. If you see fingertips on the filter,
clean it using a clean paper towel and, if needed, GC-grade methanol before remounting.

CAUTION If the filter installed is equipped with the spacer (see the kits PN 19050785 and
PN 19050786), first insert the filter, and then the spacer.

Interferential Filter

Spacer

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8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

b. Mount the photomultiplier tube assembly then fix it tightening the knurled nut.
See Figure 431.

Figure 431. Photomultiplier Tube Assembly Mounting

The result of the operation is shown in Figure 432.

Figure 432. Result of the Dual FPD Configuration

Primary Photomultiplier Tube Secondary Photomultiplier Tube

Installing of the Dual FPD and electrical connections

12. Remove the jet of the front detector control module.

a. Using the 5 mm wrench provided with the GC, loosen the jet and remove it from the
detector base body. See Figure 433.
Figure 433. FPD Jet Removal

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Performing the Dual FPD Detector Configuration

IMPORTANT Save the jet and the aluminium ring in a safe place because will be reused
when you restore the original configuration of the FPD modules.

13. Install the PT 100 simulator, provided with kit PN 19050783, on the detector base of the
front detector control module. See Figure 434.

Figure 434. PT100 Simulator Installation

PT100 Simulator provided

with the kit PN 19050783

14. Install the Dual FPD on the detector base of the detector control module placed into the
back site of the detector housing. See Figure 435.

Figure 435. Dual FPD Installation

a. Place the detector on its base body, ensuring that the aluminium ring has been
inserted in the correct position, then tighten the fixing nut.

15. Connect the cables to the FPD detectors.

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8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

a. Connect the signal, excitation voltage and ignition/heating cables coming from the
control module installed into the back site to the primary FPD detector.
See Figure 436.

Figure 436. Primary FPD Detector Cables Connection

Excitation Voltage Cable Signal Cable Ignition Heating Cable

b. Connect the signal, excitation voltage and ignition/heating cables coming from the
control module installed into the front site to the secondary FPD detector.
See Figure 436.

Figure 437. Secondary FPD Detector Cables Connection

Excitation Voltage Cable Signal Cable Ignition Heating Cable

Restart the GC and set the working parameters

16. If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.

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 8 Installing Optional Kits
Performing the Dual FPD Detector Configuration

17. Power on the GC.

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.

Set the working parameters

18. Set the parameters for the primary FPD detector (back).
a. Set the flow of the gases, the temperatures, the ignition threshold, and the PMT
voltage for the primary photomultiplier tube according to the working analytical
conditions.
19. Set the parameters for the secondary FPD detector (front).
a. Set the flow of the gases and the temperatures to Off.
b. Set the PMT voltage for the secondary photomultiplier tube.
20. Turn on the Flame On parameter of the primary FPD detector.

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 9

Adding Modules
This chapter describes how to install any added injector, detector, external, module that is
available for the TRACE 1300/TRACE 1310. See the TRACE 1300 and TRACE 1310 Spare
Parts Guide for information about ordering the equipment in this chapter.

Contents
• Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module
• Adding a GSV Injector Module
• Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module
• Adding a NPD Detector Module
• Adding an Aux Temperature/Cryo Module
• Adding a Helium Saver Injector Module
• Adding a PDD Module
• Adding a Generic Detector Interface
• Adding an Analog Output Interface

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9 Adding Modules
Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module

Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module

This section provides instructions for adding a front/back SSL, SSLBKF, PTV, or PTVBKF
injector module. According to the configuration of your TRACE 1300/TRACE 1310, a
dummy module is present into the free site where the injector module is not installed.
See Figure 438.

Figure 438. Add a New Front/Back Injector Module

Dummy Front Injector Module Dummy Back Injector Module

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

 To add an SSL, SSLBKF, PTV, or PTVBKF injector module

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.

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 9 Adding Modules
Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module

6. Put the autosampler away if present.

7. Remove the dummy module from the position where the injector module will be
installed.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat into the
injector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connections by unscrewing its fixing screw
using a T20 Torxhead screwdriver. See Figure 439.

Figure 439. Injector Gas Block Plug

Gas Block Plug on Injector Seat

O-ring

Gas Connection Without Gas Block Plug

WARNING Make sure the o-ring is placed into its seat on the gas connection.
See Figure 439. Do not install the module if the o-ring is missing.

8. Open the front door of the GC.

9. Plug the injector module into the main frame.
a. Open the module flap cover.
b. Keeping the module flap cover open, place the module in its seat. Be sure to insert the
25-pin male connector, on the bottom of the module, into the 25-pin female
connector on the injector seat of the injector housing.

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9 Adding Modules
Adding a SSL, SSLBKF, PTV, or PTVBKF Injector Module

c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.

ATTENTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

e. Keep the plug connected to the bottom.
10. Open the gas supplies.
11. Check the gas supply for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check each fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.
c. Repeat this process until all connections are leak free.
12. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
13. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
14. Pressurize the module with the carrier gas.
15. Check the module gas connections for leaks.
16. Remove the plug from the bottom.
17. Connect the analytical column end to the injector and verify the connection point.
a. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in Table 16.

Table 16. Column Insertion Depth For SSL, SSLBKF, PTV, and PTVBKF Injectors
SSL SSLBKF PTV PTVBKF
5 mm (splitless) 5 mm (splitless) 30 mm 30 mm
10 mm (split) 10 mm (split) As far as possible into
the bottom when the
PTV is used as an
On-Column injector.

18. Close the front door of the GC.

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 9 Adding Modules
Adding a GSV Injector Module

19. If present, move the autosampler towards the module to restore the original alignment.

Adding a GSV Injector Module

This section provides instructions for adding a front/back Gas Sampling Valve injector
module. According to the configuration of your TRACE 1300/TRACE 1310, a dummy
module is present into the free site where the injector module is not installed.
See Figure 440.

Figure 440. Add a New Front/Back GSV Injector Module

Dummy Front Injector Module Dummy Back Injector Module

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

 To connect the gas sampling valve module to the TRACE 1300/1310 GC

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.

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9 Adding Modules
Adding a GSV Injector Module

b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Put the autosampler away if present.
7. Remove the dummy module from the position where the injector module will be
installed.
a. Open the module flap cover.
b. Using a T20 Torxhead screwdriver, unscrew the three captive fixing screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat into the
injector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connections by unscrewing its fixing screw
using a T20 Torxhead screwdriver. See Figure 441.

Figure 441. Injector Gas Block Plug

Gas Block Plug on Injector Seat

O-ring

Gas Connection Without Gas Block Plug

WARNING Make sure the o-ring is placed into its seat on the gas connection.
See Figure 439. Do not install the module if the o-ring is missing.

8. Open the front door of the GC.

9. Plug the Gas Sampling Valve module into the main frame.

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 9 Adding Modules
Adding a GSV Injector Module

a. Open the module flap cover.

b. Keeping the module flap cover open, place the module in its seat. Be sure to insert the
25-pin male connector, on the bottom of the module, into the 25-pin female
connector on the detector seat of the detector housing.
c. Use a T20 screwdriver to tighten the three captive fixing screws without
overtightening.

Note Tighten the center screw first and then secure the side screws.

d. Close the module flap cover.

10. Connect the analytical column end insert it as far as goes and withdrawn about 2 -3 mm.
See the section “Installing the Column the First Time” on page 57.

Note If you need the backflush, connect the backflush system into the GC oven
following the instructions provided in the section Connecting the GSV
Backflush System in Chapter 8 of the TRACE 1300/TRACE 1310 Hardware
Manual.

11. Close the front door of the GC.

12. Remove the protective caps from the Sample In and Sample Out fittings. See Figure 442.

Figure 442. Protective Caps

13. Connect the Sample In and Sample Out lines.

a. By using the proper 1/8-in. tubing, nut and ferrule, connect the inlet sample line to
the Sample IN port on the GSV module. See Figure 443.

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9 Adding Modules
Adding a GSV Injector Module

Figure 443. Sample In Line Connection

IN

b. By using the proper 1/8-in. tubing, nut and ferrule, connect the Sample OUT port
on the GSV module to an exhaust system. See Figure 444.

Figure 444. Sample Out Line Connection

OUT

14. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
15. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
16. Open the module flap cover.

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 9 Adding Modules
Adding a GSV Injector Module

17. Remove the relief pins from the valve body.

IMPORTANT The valve is delivered with relief pins. These pins MUST BE removed from
the valve body before working with the valve.

Relief Pins

WARNING DO NOT SET THE CARRIER GAS INPUT PRESSURE HIGHER

THAN 450 kPa (65 psig; 4.5 bar), OTHERWISE THE VALVE MAY BE DAMAGED.

WARNING DO NOT USE HYDROGEN AS CARRIER GAS. The module is not

compatible with Hydrogen carrier gas. The same gas used as carrier gas is used to purge
and to actuate the valve and must be an inert gas.

a. Open the gas supplies.

b. Adjust the gas pressure to 450 kPa (65 psig; 4.5 bar), actuate the valve and remove the
pins.
You may need to increase the pressure a little to ease hand removal of the pins.
Remember, make sure to properly readjust the actuating operating pressure after
removing the pins, if you had to change it. Keeps these pins in a safe place.
18. For critical applications, where air diffusion must be the lowest possible, we suggest to
replace the purge vent tubing with the longer one provided. See Figure 445.

Figure 445. Purge Vent Tubing

Purge Vent Tubing

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9 Adding Modules
Adding a GSV Injector Module

a. Remove the purge valve tubing from the Purge Out port on the valve body.
b. Connect to the 24-in. long tubing from the Purge port on the valve body by using
the proper nut and ferrule.
19. Close the module flap cover.
20. If present, move the autosampler towards the to restore the original alignment.

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 9 Adding Modules
Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module

Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module

This section provides instructions for adding a front/back FID, TCD/TCD In-Series, ECD,
or FPD detector module. According to the configuration of your TRACE 1300/TRACE
1310, a dummy module is present into the free site where the detector module is not installed.
See Figure 446.

Figure 446. Add a Front/Back Detector Module

Dummy Front Detector Module Dummy Back Detector Module

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

 To add a new FID, TCD/TCD In-Series, ECD, or FPD detector module

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.

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9 Adding Modules
Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module

6. Remove the dummy module from the position where the detector module will be
installed.

ATTENTION The FPD detector must be installed in the back site of the detector housing.
For details about the installation of the FPD detector module, see the section “Installing
the FPD Detector” on page 176.

a. Open the module flap cover.

b. Using a T20 Torxhead screwdriver, unscrew and remove the two captive fixing
screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
injector/detector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connections by unscrewing its fixing screw
using a T20 Torxhead screwdriver. See Figure 447.

Figure 447. Detector Gas Block Plug

Gas Block Plug on Detector Seat

O-rings

Gas Connections Without Gas Block Plug

WARNING Make sure all the four o-rings are placed into their seats on the gas
connection. See Figure 447.

Do not install the module if the o-rings are missing.

7. Open the front door of the GC.

8. Plug the detector module into the main frame.
a. Open the module flap cover.

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 9 Adding Modules
Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module

b. Keeping the module flap cover open, place the module in its seat. Be sure to insert the
25-pin male connector, on the bottom of the module, into the 25-pin female
connector on the detector seat of the detector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.
.

ATTENTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

9. Remove the plug and connect the analytical column end to the detector.
a. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in Table 17.
Table 17. Column Insertion Depth For FID, NPD, TCD, ECD, and FPD Detectors
FID NPD TCD ECD FPD
Insert the column as far as goes and withdrawn about 23 mm 125 mm
2 -3 mm

Note If you are installing an In-Series Connection TCD module (TCD module
modified for the connection in-series with a second detector or with a
methanizer), you must connect the vent line metal tubing, that protrudes into
the GC oven, to a FIDsecond detector proceeding as follows:
• Being careful not to create too narrow angles, bend the vent line metal
tubing until its end reaches the bottom of the second detector.
• Making sure that the end of the vent line metal tube is perfectly straight, insert it
like as an analytical column through the proper detector retaining nut and
ferrule.
ATTENTION If you are connecting the In-Series Connection TCD module to a
FID, NPD, or FPD, position the vent line metal tubing so that the end of the
tubing extends the proper distance above the end of the ferrule as reported in
Table 17.
If you are connecting the In-Series Connection TCD module to an ECD, the
insertion depth of vent line metal tubing must be 21 mm.

10. Open the gas supplies.

11. Check the gas supply for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check each fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.

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9 Adding Modules
Adding a FID, TCD/TCD In-Series, ECD, or FPD Detector Module

c. Repeat this process until all connections are leak free.

12. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
13. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
14. Check the module gas connections for leaks.
15. Close the front door of the GC.

434 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 9 Adding Modules
Adding a NPD Detector Module

Adding a NPD Detector Module

This section provides instructions for adding a front/back NPD detector module. According
to the configuration of your TRACE 1300/TRACE 1310, a dummy module is present into
the free site where the detector module is not installed. See Figure 448.

ATTENTION This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

Figure 448. Add a Front/Back NPD Module

Dummy Front Detector Module Dummy Back Detector Module

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

The NPD detector module addition also requires the installation of the NPD Thermionic
Source Power Module. See Figure 449.

Figure 449. NPD Thermionic Source Power Module

The module includes the following connections. See Figure 450.

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9 Adding Modules
Adding a NPD Detector Module

Figure 450. NPD Thermionic Source Power Module Connections

1 2 3

1. AC input connector.
The module has a power rating of 120/230 Vac; 50/60 Hz; 65VA.
2. A 5-pin connector marked NPD Module (2) for the connection of the signal cable
coming from the NPD detector module.
3. A 2-pin connector marked NPD Source (3) for the connection of the thermionic source
assembly cable coming from the NPD detector module.

One protecting fuse is present inside the module. See Table 18.
Table 18. Aux Temperature/Cryo Module Protecting Fuses
Fuse Type Protections
F1 F4A 250V; (5 x 20 mm) Circuit for the generation of the current for the thermionic source

 To add a new NPD detector module

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the dummy module from the position where the detector module must be
installed.
a. Open the module flap cover.

436 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 9 Adding Modules
Adding a NPD Detector Module

b. Using a T20 Torxhead screwdriver, unscrew and remove the two captive fixing
screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
injector/detector housing. Place the dummy module on a clean surface.
d. Remove the gas block plug from the gas connections by unscrewing its fixing screw
using a T20 Torxhead screwdriver. See Figure 451.

Figure 451. Detector Gas Block Plug

Gas Block Plug on Detector Seat

O-rings

Gas Connections Without Gas Block Plug

WARNING Make sure all the four o-rings are placed into their seats on the gas
connection. See Figure 451.

Do not install the module if the o-rings are missing.

7. Open the front door of the GC.

8. Plug the detector module into the main frame.
a. Open the module flap cover.
b. Keeping the module flap cover open, place the detector module in its seat. Be sure to
insert the 25-pin male connector on the bottom of the module into the 25-pin
female connector on the detector seat of the detector housing.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.

ATTENTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

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9 Adding Modules
Adding a NPD Detector Module

9. Install the NPD Thermionic Source Power Module.

The module should be installed into an external module housing provided on the back
of the GC. See Figure 452.

Figure 452. External Modules Housing

External Modules Housing

10. Remove the cover of the external modules housing where installing the module. See
Figure 453.

Figure 453. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

a. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws.
b. Remove the covers from the housing.
11. Install the module into the housing.
a. Loosen the two hexagonal screws under the module. See Figure 454.

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 9 Adding Modules
Adding a NPD Detector Module

Figure 454. Module Installation (1)

Hexagonal Screws

b. Place the module into the left or right housing until the hexagonal screws couple the
slots on the floor of the GC. See Figure 455.

Figure 455. Module Installation (2)

Slots

c. Fingertighten the hexagonal screws slightly, or using a 10-mm wrench.

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the auxiliary module when necessary.

The result of the installation is shown in Figure 456.

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9 Adding Modules
Adding a NPD Detector Module

Figure 456. NPD Thermionic Source Power Module Installed into the GC

Cables Holder
NPD Thermionic Source Power Module

12. Connect the NPD Thermionic Source Power Module.

Tip To avoid the contact with the hot air from the vents, it is suggested to gather the
electrical cables into cables holder.

a. Open the module flap cover.

b. Using the cable provided, connect the source assembly connector on the NPD
Thermionic Source Power Module to the thermionic source and twist the ring to lock
the connection. See Figure 457.

Figure 457. Thermionic Source Assembly Cable Connection

440 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 9 Adding Modules
Adding a NPD Detector Module

c. Using the cable provided, connect the signal connector on the NPD Thermionic
Source Power Module to the connector on the detector module. See Figure 458.

Figure 458. Signal Cable Connection

13. Remove the plug and connect the column end to the detector.
14. Open the gas supplies.
15. Check the gas supply for leaks.
a. Use a handheld electronic leak detector (Thermo Scientific GLD Pro leak detector or
equivalent) to check each fitting for leaks.
b. If you detect a leak, tighten the connection and retest it.
c. Repeat this process until all connections are leak free.
16. Plug the power cable to the AC input connector of the NPD Thermionic Source Power
Module, and to the wall outlet. The LED marked On blinks green.

IMPORTANT The NPD Thermionic Source Power Module must be powered at the same
line voltage of the main GC system.

If other external modules are present, plug the power cable to the AC Input connector of
each module, and to the wall outlet.
17. Power on the GC.

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9 Adding Modules
Adding a NPD Detector Module

a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
18. Check the module gas connections for leaks.
19. Close the front door of the GC.
20. Set the make-up gas on.
21. Switch on the thermionic source. Hydrogen and air are automatically opened.
The LED marked On of the NPD Thermionic Source Power Module becomes solid
green.
22. With all gases on, heat the detector to 150 °C and hold for about 15 minutes, then
increase the temperature to 250 °C and hold for 15 minutes.
23. Increase the temperature to operating value (300 °C to 320 °C recommended). Allow 15
minutes for equilibration.
24. Check the NPD leakage current. If > 2.0 pA, verify the installation.

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 9 Adding Modules
Adding an Aux Temperature/Cryo Module

Adding an Aux Temperature/Cryo Module

This section provides instructions for updating your TRACE 1300/TRACE 1310 with the
Aux Temperature/Cryo module. See Figure 459.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

Figure 459. Aux Temperature/Cryo Module

The module includes the following connections. See Figure 460.

Figure 460. Aux Temperature/Cryo Module Connections

1 2 3

8 7 6 5 4

1. AC input connector.
The module has a power rating of 120/230 Vac; 50/60 Hz; 355 VA.
2. A 6-pin connector marked Heater 1 for the connection of the transfer line of a mass
spectrometer.
3. A 6-pin connector marked Heater 2 for the connection of the transfer line of a second
mass spectrometer.
4. 15-pin female connectors marked Bus for the communication with the GC.
5. A 16-pin connector marked Events for the connection of eight external solenoid valves
(24 V, 2 W max). The valves can be programmed individually during the running of the
instrument.

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9 Adding Modules
Adding an Aux Temperature/Cryo Module

6. A 2-pin connector marked Back Inlet for the connection of the solenoid valves for the
back PTV/PTVBKF cryogenic system.
7. A 2-pin connector marked Front Inlet for the connection of the solenoid valves for the
front PTV/PTVBKF cryogenic system.
8. A 2-pin connector marked Oven for the connection of the solenoid valves for the Oven
cryogenic system.

Four protecting fuses are present inside the module. See Table 19.
Table 19. Aux Temperature/Cryo Module Protecting Fuses
Fuse Type Protections
F1 F2A 250V; (5 x 20 mm) Heater 1
F2 F2A 250V; (5 x 20 mm) Heater 2
F3 T2A 250V; (5 x 20 mm) 24 V
F4 F1.6A 250V; (5 x 20 mm) 24 V solenoid valves

The module features the following:

• The valve control of the Oven cryo option.
• The valve control of the front/back PTV cryo option.
• Up two heater controls for the DSQ II, ITQ, or TSQ Quantum transfer line.
• Sixty-three timed events to automatically drive up to eight external on/off solenoid valves.

The module should be installed into a free external module housing provided on the back of
the GC. See Figure 461.

Figure 461. External Modules Housing

External Modules Housing

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 9 Adding Modules
Adding an Aux Temperature/Cryo Module

 To add an Aux Temperature/Cryo Module

1. Put the GC in standby condition.

2. Cool the oven, injectors, and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the cover of the external modules housing where installing the module.
See Figure 462.

Figure 462. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

a. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws.
b. Remove the covers from the housing.
7. Install the module into the housing
a. Loosen the two hexagonal screws under the module. See Figure 463.

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Figure 463. Module Installation (1)

Hexagonal Screws

b. Place the module into the left or right housing until the hexagonal screws couple the
slots on the floor of the GC. See Figure 464.

Figure 464. Module Installation (2)

Slots

c. Finger-tighten the hexagonal screws slightly, or using a 10-mm wrench.

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the auxiliary module when necessary.

The result of the installation is shown in Figure 465.

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Figure 465. Aux Temperature/Cryo Module Installed into the GC

Cables Holder
Aux Temperature/Cryo Module

8. Connect the Aux Temperature/Cryo module.

Tip To avoid the contact with the hot air from the vents, it is suggested to gather the
electrical cables into cables holder.

a. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
– If a DSQ II, ITQ, or TSQ Quantum mass spectrometer is coupled with the GC,
connect the heater cable coming from the transfer line to the connector marked
Heater 1 or Heater 2.

ATTENTION An extension cable transfer line is needed when connecting an ITQ mass
spectrometer to the Aux Temperature/Cryo module.

– If the cryo option for the Oven is present, connect the 2-pin connector marked
Cryo Valves - Oven to the cryo solenoid valve using the cable provided.
– If the cryo option for the front/back PTV/PTVBKF injector is present, connect
the 2-pin connector marked Cryo Valves - Front Inlet or Cryo Valves-Back
Inlet to the cryo solenoid valve using the cable provided.
– If the on/off activation of external solenoid valves (up to eight) is required,
connect a 2-pin connector marked Event 1÷8 to each external solenoid valve.
– Plug the power cable to the AC Input connector on the front of the module and
to the wall outlet. The LED marked On lights after the GC is powered on.

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IMPORTANT The Auxiliary Temperature/Cryo module must be powered at the same

power supply voltage required for the GC.

9. Open the gas supplies.

10. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
11. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
12. Configure and enable the system installed through the user interface of your GC, or
through the CDS in use. Refer to the TRACE 1300 and TRACE 1310 User Guide.

Adding a Helium Saver Injector Module

The Instant Connect Helium Saver Injector Module (HeS-S/SL) is designed as a “double
wide” module that fits into the space provided by one detector module and one injector
module on the upper deck of your TRACE 1300/TRACE 1310 GC.

Installing the Module

The module may be placed in either the Front or Back position on the upper deck.
• If the module is located in the Front position, plumb the helium supply to the Front
Make Up gas connection on the back panel of the GC.
• If the module is located in the Back position, plumb the helium to the Back Make Up gas
connection on the back panel of the GC.

Plumb the nitrogen gas to the corresponding Front or Back Carrier gas connections.

IMPORTANT Although nitrogen will not be the actual carrier gas for the analytical
separation, it will be the carrier gas during the injection, and it is necessary to plumb the
nitrogen to the carrier gas input.

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Note For optimal results, use a high capacity oxygen trap on the nitrogen supply, and a
heated zirconium alloy gettering trap on the helium supply. These traps can be purchased
together as Thermo Scientific part number 1R120577-0001.

Alternatively, you may opt to provide own helium purification. Due to the low flow rate
of helium employed, conventional chemical traps (non-heated traps) may actually
contaminate the gas supply. Heated zirconium-based traps specifically designed for helium
are ideal. These traps can also remove nitrogen, which is difficult to eliminate from
conventional traps.

 To install the Instant Connect Helium Saver Injector Module

1. Cool the GC and MS heated zones and then shut off all carrier and detector gases on the
local GC user interface as well as at the source cylinder.
2. Remove the detector and injector “dummy modules” from the locations where the
Helium Saver S/SL module will be installed by loosening the two captive T20 screws on
each module. See Figure 466.

Figure 466. Removing the Dummy Modules

3. Remove the blanking washers from the detector and injector pneumatic network.
See Figure 467.

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Figure 467. Removing the Blanking Washers

Blanking
Washer

4. Ensure there is an o-ring present in the o-ring seat of each position on the pneumatic
network block. See Figure 468.

Figure 468. Confirming Presence of O-rings

Make-up Gas Port
Delivering Helium

5. Plumb a cylinder of high purity helium 99.999% to the appropriate make-up gas
connection on the back of the GC using the adapter provided. See Figure 469.
The Thermo Scientific gas purification kit part number 1R120577-0001 contains a
heated gettering trap which can be used directly with this fitting.

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Figure 469. Plumbing the Helium Cylinder

Alternatively, a customer supplied gettering trap can be used along with the provided
1/16 in. stainless steel tubing. Set the regulator pressure such that helium purges the
make-up line of the pneumatic network for 15 min at a flow of 20–100 mL/min.
The flow can be measured directly from the detector block at the left most port as shown
in Figure 468 on page 450. This will purge the gas line as well as eliminate air from the
gettering material of an in-line heated helium purifier. Do this before applying power to
the purifier. Ensure the hose leading to the flowmeter is clean and dust free before holding
it against the o-ring seat.
6. Carefully insert the Instant Connect Helium Saver Injector Module into position on the
GC top deck after first inserting the ceramic insulator shown in Figure 470.

Figure 470. Positioning the Instant Connect Helium Saver Injector Module

Also, be sure to remove the column nut from the injector if it has not already been
removed. This prevents damage to the ceramic insulator when the module is inserted.

Note It may be helpful to insert the left side of the module just prior to the right side
in order to clear the space properly. The module fits snugly into position but should
not require undue force to clear the sheet metal opening.

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7. Secure the T20 screws near the center line of the module leading to the injector and
detector pneumatic network prior to tightening the captive screws which hold the
module. See Figure 471.

Figure 471. Securing the Instant Connect Helium Saver Injector Module in Place

It will be necessary to push down on the right side of the module near the back edge to
ensure engagement of the 25 pin D-sub electrical connector. Wiggle the module as
necessary to engage the pins and then secure the screws.
8. At this point, the regulator that delivers the helium can be set to the appropriate pressure:
typically 110 psig (760 kPa; 7.6 bar) will suffice for 0.25 mm i.d. columns of 30 m
length.
Dial the pressure up slowly while tapping the pressure gauge in order to set the
appropriate pressure. Table 20 serves as a guide for setting the correct helium regulator
pressure.
Table 20. Determining Correct Helium Regulator Pressure for Column Type (Sheet 1 of 2)
Required helium regulator
Column Length Column i.d. Desired Flow *
pressure psig (kPa; bar) **
5 m/10 m 0.10 mm 0.4 mL/min 110 psig (760 kPa; 7.6 bar)
0.5 mL/min 120 psig (830 kPa; 8.3 bar)
0.6 mL/min 130 psig (900 kPa; 9 bar)
10 m/20 m 0.18 mm 0.8 mL/min 100 psig (690 kPa; 6.9 bar)
1.0 mL/min 110 psig (760 kPa; 7.6 bar)
1.2 mL/min 120 psig (830 kPa; 8.3 bar)
1.5 mL/min 130 psig (900 kPa; 9 bar)
2.0 mL/min 140 psig (970 kPa; 9.7 bar)
15 m 0.25 mm 1.0 mL/min 100 psig (690 kPa; 6.9 bar)
1.2 mL/min 110 psig (760 kPa; 7.6 bar)
1.7 mL/min 120 psig (830 kPa; 8.3 bar)

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Table 20. Determining Correct Helium Regulator Pressure for Column Type (Sheet 2 of 2)
Required helium regulator
Column Length Column i.d. Desired Flow *
pressure psig (kPa; bar) **
2.2 mL/min 130 psig (900 kPa; 9 bar)
2.7 mL/min 140 psig (970 kPa; 9.7 bar)
30 m 0.25 mm 1.0 mL/min 100 psig (690 kPa; 6.9 bar)
1.2 mL/min 110 psig (760 kPa; 7.6 bar)
1.7 mL/min 120 psig (830 kPa; 8.3 bar)
2.2 mL/min 130 psig (900 kPa; 9 bar)
2.7 mL/min 140 psig (970 kPa; 9.7 bar)
3.2 mL/min 150 psig (1030 kPa; 10.3 bar)
60 m 0.25 mm 1.0 mL/min 120 psig (830 kPa; 8.3 bar)
1.2 mL/min 130 psig (900 kPa; 9 bar)
1.7 mL/min 140 psig (970 kPa; 9.7 bar)
2.2 mL/min 150 psig (1030 kPa; 10.3 bar)
100 m 0.25 mm 1.0 mL/min 130 psig (900 kPa; 9 bar)
1.2 mL/min 140 psig (970 kPa; 9.7 bar)
1.7 mL/min 150 psig (1030 kPa; 10.3 bar)
30 m 0.32 mm 1.5 mL/min 100 psig (690 kPa; 6.9 bar)
2.0 mL/min 110 psig (760 kPa; 7.6 bar)
2.5 mL/min 120 psig (830 kPa; 8.3 bar)
3.0 mL/min 130 psig (900 kPa; 9 bar)
3.5 mL/min 140 psig (970 kPa; 9.7 bar)
60 m 0.32 mm 1.5 mL/min 110 psig (760 kPa; 7.6 bar)
2.0 mL/min 120 psig (830 kPa; 8.3 bar)
2.5 mL/min 130 psig (900 kPa; 9 bar)
3.0 mL/min 140 psig (970 kPa; 9.7 bar)
3.5 mL/min 150 psig (1030 kPa; 10.3 bar)
100 m 0.32 mm 1.5 mL/min 130 psig (900 kPa; 9 bar)
2.0 mL/min 140 psig (970 kPa; 9.7 bar)
2.5 mL/min 150 psig (1030 kPa; 10.3 bar)
*
For flow rates not specifically listed, round up in pressure to the next highest value. For example, if
1.5 mL/min is desired using a 0.25 mm ID column of 15 m length, use a helium regulator pressure of
110 psig (760 kPa; 7.6 bar).
** Minimum
pressure required to avoid nitrogen back diffusion into the helium carrier gas. Higher pressure
will result in a slightly higher (than minimum) consumption of helium, but will not result in adverse
analytical performance.

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9. Plumb a nitrogen supply to the appropriate Carrier input at the back of the GC as shown
in Figure 472.

Figure 472. Plumbing the Nitrogen Supply to the GC

10. The Thermo Scientific gas purification kit contains a high capacity oxygen trap that
should be placed in the nitrogen line. Be sure to purge the regulator and gas line before
attaching the oxygen filter.
11. Keep nitrogen flowing through the trap while attaching the filter to the back of the GC in
order to prevent oxygen from entering the trap.
12. Adjust the pressure regulator to supply a pressure sufficient for the analytical method in
use. In general, 75 psig (517 kPa; 5.17 bar) is more than sufficient for most applications.
Very small bore capillaries may require higher pressures.

Installing the Column

The Instant Connect Helium Saver Injector Module is designed to be used with Silflow™
metallic ferrules. This allows for very accurate trimming of the column following compression
of the ferrule onto the column. It is important that only 5 mm of column protrudes past the
tip of the ferrule for proper operation. The inlet has been designed to work optimally with
0.25 mm i.d. columns, although larger (up to 0.32 mm i.d.) or narrower i.d.’s can be used
successfully.

 To install the column

1. Insert the column through the SilFlow™ nut and ferrule as shown in Figure 473.

Figure 473. Installing the Column Nut and Ferrule

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2. Allow a few centimeters of column to extend past the tip of the ferrule and insert it into
the base of the inlet. It will be necessary to gently poke around in order to find the small
bore cone that serves as a column guide and ferrule seat.
3. Use the knurled tool to tighten the nut by finger force only, until the ferrule grabs the
column, and the column no longer slides in the bore of the ferrule.
The column connection should appear as shown in Figure 474.

Figure 474. Installing the Column in the Injector

The column should be fully captured by the ferrule without the ability to slide it up or
down.
4. At this point, remove the nut and ferrule assembly and confirm the column will not slide
in the ferrule (See first Tip on page 455). Trim the column such that only 5 mm extends
past the tip of the ferrule.
5. Carefully reinstall the column and again tighten the ferrule. The column is now installed
in the injector.

Tip An indispensable tool for removal of the column from the inlet is a dental pick or
thumbtack. When the SilFlow ferrule engages the inlet on tightening, it is slightly
deformed at the tip in order for the sealing to occur. This causes the ferrule to become
“stuck,” which is a normal occurrence. The ferrule can easily be removed by inserting
the pointed tip of the dental pick gently along the side of the ferrule and pressing
vertically to cause the ferrule to be displaced to the side. Gently pulling on the
column at the same time will dislodge the ferrule.

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Note The standard outfit kit for the Instant Connect Helium Saver Injector Module is
equipped with SilFlow ferrules having internal diameters of 0.50, 0.40, and 0.35 mm.

If you are using a 0.32 mm id column, use the 0.50 mm i.d. ferrules supplied.
If you are using a 0.25 mm id column or smaller, use the 0.35 mm id ferrule if the column
will pass through it.
This will allow easy finger tightening of the ferrule using the hand tool without undue
torque. In other words, use the smallest ferrule that will fit your columns.
The ideal situation is when the column passes through the hole with slight pressure.
The replacement Thermo part numbers are as follows:
• 0.50 mm ferrule 10ea. 29063467 (for 0.32 columns)
• 0.40 mm ferrule 10ea. 29063466 (for 0.25 columns and smaller on the high end of
the o.d. tolerance)
• 0.35 mm ferrule 10ea. 29063465 (for 0.25 columns and smaller on the low end of
the o.d. tolerance).

Tip When installing the column, nut and ferrule, lift up slightly on the ceramic insulator
surrounding the base of the injector insert as shown in figure.

This will expose the end of the insert and make it easier to locate the column in the central
hole.
After the final trimming and installation of the column, make sure to pull the insulator
back to its lowest position.

Checking for Leaks

A hand held helium leak detector may be used to check for helium leaks around the inlet and
column connection. With the helium conservation enabled, approximately 4 mL/min of
helium will enter the injection port.
For maximum sensitivity of the leak detector, set the split flow to Off during leak check.
This results only in column and septum purge flows.

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The composition of the gas mixture in the inlet will be mostly helium and allow good
sensitivity. For bulk nitrogen leak detection, the GC column exit can be plugged, and the
automated pressure drop leak detection of the GC used for determining the pressure drop.

The helium delivery block should be parked in the rear “blocked” position of Figure 475 if
leak checking is done based on pressure drop. The block must be replaced in the front
position to resume operation.
Figure 475. Removing the Helium Tube from the Gas Delivery Block

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Adding a PDD Module

Adding a PDD Module

This section provides instructions for adding a front/back PDD detector module. According
to the configuration of your TRACE 1300/TRACE 1310, a dummy module is present into
the free site where the detector module is not installed. See Figure 476.

Figure 476. Add a Front/Back Detector Module

Dummy Front Detector Module Dummy Back Detector Module

ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

Getting Started
To properly installing and connecting the PDD module see sequentially the following
sections:
• “Installing a PDD Module” on page 459
• “Plumbing the Gas Lines” on page 463
• “Installing and Purging the Gas Regulator” on page 464
• “Installing and Purging the Helium Purifier” on page 464
• “Connecting the Discharge Gas Supply” on page 465
• “Connecting the High Voltage and Pulses Cables” on page 465
• “Installing the Capillary Column” on page 470
• “Installing the Packed Column” on page 470
• “Testing for Leaks” on page 470
• “Performing Initial Power Up” on page 471

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Installing a PDD Module

 To install a PDD detector module

The result of the operation is shown in Figure 477.

Figure 477. Result of the PDD Installation

Note The installation of a single PDD detector is considered. The injector modules are
deliberately missing for convenience.

1. Put the GC in standby condition.

2. Cool the oven, injector, and detector to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the dummy module from the position where the detector module will be
installed.

a. Open the module flap cover.

b. Using a T20 Torxhead screwdriver, unscrew and remove the two captive fixing
screws.

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Adding a PDD Module

c. Keeping the dummy module flap cover open, lift up the module from its seat in the
injector/detector housing. Place the dummy module on a clean surface.
d. Keep the gas block plug of the gas connections installed. See Figure 447.

Figure 478. Detector Gas Block Plug

Gas Block Plug on Detector Seat

7. Open the front door of the GC.

8. Plug and fix the detector module into the main frame.

a. Place the module in its seat. Be sure to insert the 25-pin male connector, on the
bottom of the module, into the 25-pin female connector on the detector seat of the
detector housing.

Note Because the module flap cover of the PDD detector cannot be opened, two
holes are provided on the module flap cover for accessing the captive fixing
screws. See Figure 479.

Figure 479. Holes on the Module Flap Cover

Holes

b. Insert a T20 Torxhead screwdriver into the holes provided on the module flap cover
and tighten the captive fixing screws without overtightening.
.

ATTENTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

9. Mount the support bracket of the pulse generator. See Figure 480.
Note The support bracket can hold two pulse generators for supplying two PDD
detectors on the GC.

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Figure 480. Support Bracket

Lower Clamp

Upper Clamps

a. Insert the upper clamps of the support bracket into the last slots of the cooling fan
grid. See Figure 481.
Figure 481. Support Bracket Installation (1)

Cooling Fan
Upper Clamps Grid

b. Lift the bottom part of the support bracket and push it back until the lower lamp
hooks the rear of the GC top cover. See Figure 482.

Figure 482. Support Bracket Installation (1)

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Note Two support brackets can be installed for supplying a maximum of four
PDD detectors, two installed on the GC and two installed on the TRACE 1310
Auxiliary Oven. See the example of Figure 483 and Figure 484.

Figure 483. Installation of two Support Bracket

Figure 484. Installation of Four PDD Detectors

10. Place the pulse generator on the support bracket.

a. Place the pulse generator on a convenient position of the support bracket by aligning
the four holes on the base of the generator with the corresponding four rivets on the
support bracket as shown in the example of Figure 485.

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Figure 485. Pulse Generator Installation

Base Holes

Pulse
Generator

Rivets

Barrel Nut

b. Fix the pulse generator using the four barrel nuts provided. Tighten the nuts using a
T30 Torxhead screwdriver.

Plumbing the Gas Lines

Figure 486 shows the diagram of the gas lines connections of the detector system.

Figure 486. Gas Line Connections

Before connecting the gas lines verify that:

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• The pressure regulators are commercial ultra-pure grade regulators with stainless steel
diaphragms. Regulators with diaphragms made of neoprene or others elastomers should
never be used.
• The connecting tubes are thoroughly cleaned and baked before use.
• The gas regulator and the helium purifier must be properly purged. See the following
sections for further details: “Installing and Purging the Gas Regulator” on page 464,
“Installing and Purging the Helium Purifier” on page 464, and “Connecting the
Discharge Gas Supply” on page 465.

WARNING Never use leak detecting fluids on any part of the system.

Installing and Purging the Gas Regulator

WARNING To avoid injury, before starting make sure to strictly respect all the
Regulations concerning the manual handling of gas cylinders under pressure.

 To install and purge the gas regulator

1. Install the pressure regulator on the cylinder.

2. Making sure the regulator is closed, open the cylinder allowing the regulator to pressurize.
3. Close the cylinder and check the regulator for pressure leaks. Observe the needle of the
regulator output pressure gauge for 15 minutes.
4. Open the regulator allowing the pressure to relief.
5. Repeat five times from step 2.

Installing and Purging the Helium Purifier

 To install and purge the helium purifier

Before starting, make sure the helium purifier outlet tube is capped.
1. Connect the helium cylinder pressure regulator to the inlet port of the helium purifier by
using the appropriate connecting tube and fittings.
2. Turn the output pressure regulating knob clockwise until the gauge registers 345 kPa
(50 psig).
3. Wait five minutes for equilibrium, then turn the regulating knob all the way
counterclockwise.

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4. Observe the needle of the output pressure gauge for 15 minutes. There will be a slight
initial drop. If it does not move after that, consider all the connections are tight.
5. If necessary, use an electronic leak detector to locate any leaks. If a leak detector is not
available, tighten all the fitting and repressurize the system for another test.
6. Uncap the outlet tube of the helium purifier and purge the system for 15 to 30 minutes at
60 - 80 mL/min to eliminate air from the purifier getting material.

Connecting the Discharge Gas Supply

 To connect the discharge gas supply

1. Connect the helium purifier outlet port to a port of the Tee connector provided by using
the 1/16-in. o.d. connecting tube provided.
2. Connect the second port of the Tee connector to the carrier gas inlet port, located on the
rear panel of the GC, by using a sufficient piece of the stainless steel connecting tube
provided and the appropriate fitting.
3. Connect the last port of the Tee connector to the inlet of the discharge gas restrictor.
4. Connect the outlet tube of the restrictor to a flow measuring device and adjust the helium
pressure to obtain a flow of about 30 mL/min.
5. After setting the flow rate, connect the outlet of the restrictor to the discharge gas inlet on
the PDD module. See Figure 487.
Figure 487. Gas Supply Connections

Connecting the High Voltage and Pulses Cables

 To connect the cables from the pulse generator to the PDD module

WARNING Never the high voltage discharge cable must be disconnected when the pulse
generator is turned on. Dangerous high voltage is present: 3700 V Peak; 170 mA Peak.

To avoid accidental disconnection of the high voltage discharge cable (mainly) and the
pulses cable from the pulse generator, the safety covers must be installed. See Figure 488.

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Figure 488. Pulse Generator Safety Covers

Safety Cover
Pulse Generator

Safety Cover

1. Connect the high voltage discharge cable.

a. On the high voltage side, loosen the right nut that fix the pulse generator to the
bracket. Do not loosen the left nut, keep it tightened. Place the safety cover until the
nuts couple the slots on the bottom of the safety cover. See Figure 489.

Figure 489. High Voltage Discharge Cable Connection (1)

b. Tighten the right nut using a T30 torxhead screwdriver. See Figure 490.

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Figure 490. High Voltage Discharge Cable Connection (2)

c. Insert the connector of the high voltage discharge cable, coming from the high
voltage discharge electrode on the PDD module, into the connection provided in the
pulse generator passing through the hole on the safety cover. See Figure 491.

Figure 491. High Voltage Discharge Cable Connection (3)

High Voltage
Discharge Cable

High Voltage
Discharge
Electrode

High Voltage
Discharge Cable

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2. Connect the pulses cable.

a. Plug the 4-pin pulses cable connect into the 4-pin connector on the pulse generator
See Figure 492.

Figure 492. Pulses Cable Connection (1)

b. On the pulse generator control side, loosen the right nut that fix the pulse generator
to the bracket. Do not loosen the left nut, keep it tightened. Place the safety cover
until the nuts couple the slots on the bottom of the safety cover payning attention to
guide the cable into the slot located on the bottom of the safety cover. See Figure 493.

Figure 493. Pulses Cable Connection (2)

Slot

c. Tighten the right nut using a T30 torxhead screwdriver. See Figure 494.

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Figure 494. Pulses Cable Connection (3)

d. Connect the cable between the pulse generator and the pulse generator control on the
PDD module. See Figure 495.

Figure 495. Pulses Cable Connection (4)

Pulses Generator Control

Connector

WARNING If for any reasons it is necessary to disconnect the cables from the pulse
generator, the safety covers must be removed proceeding in the reverse order in which they
was installed. Before starting, make sure that the GC is powered Off.

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Installing the Capillary Column

For PDD, the column must penetrate 136 mm inside the pre-installed capillary column
adapter.

 To install the capillary column

1. Make a mark on the column 136 mm from the end.

2. Remove the knurled nut column inlet at the bottom of the detector. Slide the nut overt
the end of the column, followed by the appropriate column ferrule.
3. Seat the ferrule in the detail of the column adapter and begin sliding the column through
the capillary column adapter and into the column inlet.
4. Get the nut started on the threads and tighten it until you feel it contact the ferrule, then
back off half a turn.
5. Slide the column into the column inlet until the mark is flush with the surface of the
knurled nut, and secure the column in the adapter by tightening the knurled nut finger
tight only.

Installing the Packed Column

 To install the packed column

1. Remove the pre-installed capillary column adapter.

2. Replace pre-installed capillary column adapter with the packed columns adapter that
penetrates into the PDD cell for the correct length.
3. Connect the packed column to the packed column adapter.

Testing for Leaks

It is critical for the system to be leak-tight. Leak test is strongly recommended before
operating with PDD.

 To test for leaks

1. Open the discharge gas supply.

2. Cap the tube and pressurize the entire system with helium to 138 kPa (20 psi).
3. If the system does not hold pressure, check all the fittings with an electronic helium leak
detector. DO NOT use leak detecting liquids.
4. Tighten fittings as required.

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Performing Initial Power Up

 To perform the initial power up

WARNING During normal operation, the detector produce ultraviolet energy (UVA,
UVB), some of which may be emitted. Do not watch the arc without eye protection.

1. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
2. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
3. Close the front door of the GC.
4. Set the detector temperature to 100 °C and allow time for the detector to reach the set
temperature, and for the helium purifier to reach the optimum working condition.
5. Turn on the pulse generator.
6. Check the standing/background current. Optimum range is 600 - 2000 pA at 100°C.
Lower current indicates a clean, leak-free system.
7. The recommended detector temperature is 20 °C above the column temperature, with a
minimum of 100 °C. Set the detector to the operating temperature required for the
intended analysis. When the detector has reached the set temperature, read and record the
standing current.
8. Start carrier flow, then read the standing current.The difference between this reading and
the one previous is the ionization of the combined impurities in and eluting with the
carrier gas. The smaller the difference, the better the quality of the gas exiting the column.
9. Set the column oven to the temperature required for the intended analysis. When the
oven reaches the set temperature, read the standing current. The difference with the
previous reading is the ionization of the column bleed. The smaller the difference, the
better the column is conditioned.
From this point, the standing current should be observed and logged after any system
change. In addition, logging the standing current (with and without the column) on a
regular basis is an effective monitor of system integrity (leak tightness and cleanliness).
We also recommend tracking the internal standard (quantity on column/area count) for
sensitivity continuity.

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Adding a Generic Detector Interface

This section provides instructions for installing the detector and control modules of the
Generic Detector Interface on your TRACE 1300/1310 GC or TRACE 1310 Auxiliary
Oven.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

Preliminary Operations
Before starting, the following preliminary operation must be carried out.
1. Put the GC in standby condition.
2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

CAUTION If the GC is coupled to a TRACE 1310 Auxiliary Oven, unplug the power
cable from the AC Input connector into the back of the TRACE 1310 Auxiliary Oven
and from the wall outlet.

4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.

Getting Started
For properly installing the Generic Detector Interface see the following sections:
• “Removing the GC Back Cover” on page 473
• “Assembling the GDI Electrical Interface” on page 474
• “Replacing the Encapsulated Flow Restrictors” on page 476
• “Installing and Connecting the GDI Electrical Interface” on page 477
• “Connecting a GDI Interface to the TRACE 1310 Auxiliary Oven” on page 481

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• “Installing a GDI Mechanical Module” on page 482

• “Connecting the Detector Gas Tubings to the Manifolds” on page 484
• “Connecting Heater and Signal Cables” on page 488
• “Restarting the GC” on page 489
• “Performing the Third-party Detector Start-up and Optimization” on page 489
• “Configuring and Setting GDI Detector” on page 490

Removing the GC Back Cover

Remove the back cover for accessing the compartment on the back of the GC.

 To remove the back cover

1. Remove the cover.

a. Using a T20 Torxhead screwdriver, unscrew the four screws that secure the back cover
to the GC. See Figure 496.

Figure 496. Back Cover Removal

Back Cover Fixing Screws

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back cover.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

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Assembling the GDI Electrical Interface

If not already done, you must connect the detector cable and its cable grommet into the GDI
electrical interface. See Figure 497.

Figure 497. GDI Electrical Interface Back View

Duct for Detector Cable

Detector Cable

 To assemble the GDI electrical interface

1. Unscrew the fixing screws of the GDI electrical interface using a T10 torxhead
screwdriver, and remove the top cover. See Figure 498.

Figure 498. Assemble GDI Electrical Interface (1)

2. On the board inside the control module look for the 26-pin male connector marked J4.
See Figure 499.

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 9 Adding Modules
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Figure 499. Assemble GDI Electrical Interface (2)

J4

Connector J4

3. Connect the 26-pin female connector of the detector cable to the 26-pin male connector
marked J4 on the board.
4. Guide the detector cable and its cable grommet out of the control module through the
duct. See Figure 500.

Figure 500. Assemble GDI Electrical Interface (3)

F1

Detector Cable
F2

ATTENTION Two protecting fuses F1 and F2 are present on the electronic board of the
GDI electrical interface for the protection of the 24 Vdc and 48 Vac respectively:
• F1 = T300mA 250V; (5 x 20 mm)
• F2 = T2A 250V; (5 x 20 mm)

5. Remount and fix the top cover by using the fixing screws.

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Replacing the Encapsulated Flow Restrictors

This section provides instruction for replacing the 1/4-in. OD x 1/4-in. length encapsulated
flow restrictors. The flow restrictors are located inside the manifold marked Gas Outputs on
the GDI electrical interface. See Figure 501.

Figure 501. Gas Outputs Manifold

Gas Outputs
Manifold

 To replace an encapsulated flow restrictor

1. Remove the Gas Outputs manifold from the GDI electrical interface by unscrewing its
fixing screws using a T20 Torxhead screwdriver. See Figure 502.

Figure 502. Gas Outputs Manifold

Pay attention to not lose
the o-ring placed into the
manifold

Manifold O-Rings

2. Remove the encapsulated flow restrictor of interest from its seat using tweezers.
See Figure 503.

Figure 503. Flow Restrictor Replacement

Pay attention to not
lose the internal
Encapsulated o-ring
Flow
Restrictors
Internal O-Rings

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 9 Adding Modules
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3. Replace the encapsulated flow restrictor with the ones required by the third-party
detector, then reinstall the manifold proceeding in the reverse order in which it was
removed.
4. Calibrate the new full scale of the flow restrictor.

a. In the GDI Configuration page, set gas type to the real gas in use and full scale to
100 mL/min whatever restrictor is installed.
See the section “Configuring and Setting GDI Detector” on page 490, and refer to
Chapter 2 and Chapter 4 of the TRACE 1300/TRACE 1310 User Guide.
b. Set relevant channel flow rate in the Method/Instrument Control page to the full
scale value (100 mL/min). Make sure the gas input pressure is at least 60 psig
(414 kPa), and verify that the actual flow displayed by the GC reaches 100 mL/min
as set.
See the section “Configuring and Setting GDI Detector” on page 490, and refer to
Chapter 2 and Chapter 4 of the TRACE 1300/TRACE 1310 User Guide.
c. Check the real flow rate with an external flowmeter.
d. Use the real reading of the flowmeter as the new full scale, and set it in the GDI
Configuration page.

Installing and Connecting the GDI Electrical Interface

This section provides the instruction for installing and connecting the GDI electrical
interface.

CAUTION It is mandatory that the GDI electrical interface must be placed into an
housing on the back of the GC. If both the housings are already occupied by other
external modules, one of these must be removed and placed beside the GC, and in the
most comfortable position for the user to have free access to the connections.

 To install and connect the GDI electrical interface

1. Remove the cover of the external modules housing where installing the module.
See Figure 504.

Figure 504. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

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9 Adding Modules
Adding a Generic Detector Interface

a. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws, then remove the cover from the housing.
2. Install the GDI electrical interface into the housing
a. Loosen the two hexagonal screws under the module. See Figure 505.

Figure 505. GDI Module Installation (1)

Hexagonal Screws

b. Carefully place the GDI electrical interface into the left or right housing. Guide the
detector cable into the electronic compartment of the GC, next push the module
until the hexagonal screws couple with the slots on the floor of the GC.
See Figure 506.
Figure 506. GDI Module Installation (2)

Slots

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 9 Adding Modules
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c. Finger-tighten the hexagonal screws slightly, or use a 10-mm wrench.

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the GDI electrical interface when necessary.

ATTENTION If an GDI mechanical module will be installed on the TRACE

1300/TRACE 1310 GC, continue the procedure from the step 3. If an GDI mechanical
module will be installed on the TRACE 1310 Auxiliary Oven, jump to the section
“Connecting a GDI Interface to the TRACE 1310 Auxiliary Oven” on page 481.

3. Connect the 26-pin connector of the detector cable coming from the GDI electrical
interface to the backplane board in the electronic compartment.
a. On the backplane board disconnect the flat cable from the connector marked J13
DET.FRONT if the GDI mechanical module is installed in the Front site, or from
the connector marked J5 DET.REAR if the GDI mechanical module is installed in
the Back site. See Figure 507.

Figure 507. GC Backplane Board Layout

J5 DET.REAR Connector
J13 DET.FRONT Connector

b. Connect the detector cable to the connector J13 DET.FRONT, or J5 DET.BACK

accordingly.

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ATTENTION TRACE 13100/1310 coupled with a TRACE 1310 Auxiliary Oven

If you are installing the GDI mechanical module in the Front or Back site of a TRACE
1300/1310 GC coupled with a TRACE 1310 Auxiliary Oven, the detector cable of the
GDI electrical interface must be connected as follows:
• Mechanical module installed in the Front site: On the backplane board disconnect
the flat cable from the connector marked J13 DET.FRONT. Connect the detector
cable to the connector J13 DET.FRONT.
• Mechanical module installed in the Back site: Do NOT DISCONNECT the flat
cable from connector marked J5 DET.REAR. Connect the detector cable directly to
the additional connector on the flat cable.

4. Reconnect the ground wire to the back cover terminal.

5. Replace the back cover of the GC proceeding in the reverse order in which it was
removed.
The result of the installation is shown in Figure 508.

Figure 508. GDI Electrical Interface Installed into the GC

GDI electrical interface

Note Continue the procedure of installation following the instruction reported in the
section “Installing a GDI Mechanical Module” on page 482.

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Connecting a GDI Interface to the TRACE 1310 Auxiliary Oven

The GDI mechanical module can be installed as auxiliary detector into the position Aux L or
Aux R of the auxiliary detector assembly.

The detector cable of the GDI electrical interface, installed into an external module housing
on the back of the GC, must be connected to the VOBP-HRM board located into the
electronic compartment of the TRACE 1310 Auxiliary Oven. See the following procedure.

 To connect the GDI electrical interface to the TRACE 1310 auxiliary oven

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

Before starting make sure that the TRACE 1300/1310 GC and the TRACE 1310
Auxiliary Oven are powered off and the power cables disconnected.

1. Carefully decouple the TRACE 1310 Auxiliary Oven from the GC for creating enough
operating space for guiding the detector cable from the GDI electrical interface through
the GC into the electronic compartment of the TRACE 1310 Auxiliary Oven.

WARNING Before proceeding this operation make sure to disconnect properly the inner
tube. Refer to the section Coupling the TRACE 1310 Auxiliary Oven to the GC on the
TRACE 1310 Auxiliary Oven Instruction Manual, and operating in the reverse order in
which it was mounted.

2. In the electronic compartment look for the VOBP-HRM board. It is located over the
power section of the TRACE 1310 Auxiliary Oven. See Figure 509.
Figure 509. TRACE 1310 Auxiliary Oven: VOBP-HRM Board

VOBP-HRM

3. Connect the 26-pin connector of the detector cable coming from the GDI electrical
interface to the VOBP-HRM board in the electronic compartment.
a. On the board VOBP-HRM disconnect the flat cable from the connector marked J29
AUX DET LEFT if the GDI mechanical module is installed in the Left site, or from
the connector marked J13 AUX DET RIGHT if the GDI mechanical module is
installed in the Right site. See Figure 510.

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9 Adding Modules
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Figure 510. VOBO-HRM Board Layout

AUX DET LEFT

AUX DET RIGHT
J29 J13

b. Connect the detector cable to the connector J29 AUX DET LEFT, or J13 AUX
DET RIGHT accordingly.
detector

4. Re-couple the TRACE 1310 Auxiliary Oven to the GC.

Note Continue the procedure of installation following the instruction reported in the
section “Installing a GDI Mechanical Module” on page 482.

Installing a GDI Mechanical Module

This section provides instructions for adding a Front/Back/Aux L/Aux R GDI mechanical
module. According to the configuration of your TRACE 1300/TRACE 1310 GC, a dummy
module is present into the free site where a detector module is not installed. See Figure 446.

Figure 511. Add a Front/Back/Aux L/Aux R Detector Module

Dummy AUX L
Detector Module

Dummy AUX R
Detector Module

Dummy Back
Detector Module

Dummy Front
Detector Module

TRACE 1300/TRACE 1310 GC TRACE 1310 Auxiliary Oven

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 9 Adding Modules
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ATTENTION Where a dummy module is installed, the gas connection is blocked by a

plug.

 To install a GDI mechanical module

1. Remove the dummy module from the position where the detector module will be
installed.

a. Open the module flap cover.

b. Using a T20 Torxhead screwdriver, unscrew and remove the two captive fixing
screws.
c. Keeping the dummy module flap cover open, lift up the module from its seat in the
injector/detector housing. Place the dummy module on a clean surface.
d. DO NOT REMOVE the gas block plug from the gas connections. See Figure 512.
The gas supply is done through the GDI electrical interface.

Figure 512. Detector Gas Block Plug (1)

Gas Block Plug on Detector Seat

CAUTION In the case you are removing a real detector module instead of a dummy
module, you must place and fix the gas block plug on the gas connections using a T20
Torxhead screwdriver. See Figure 513.

Figure 513. Detector Gas Block Plug (2)

Gas Block Plug

Gas Connections
Without Gas Block Plug

2. Plug the GDI mechanical module into the main frame.

a. Open the module flap cover.

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b. Keeping the module flap cover open, place the module in its seat. Note that the
25-pin female connector on the detector seat of the detector housing is not used.
c. Use a T20 Torxhead screwdriver to tighten the three captive fixing screws without
overtightening.
.

ATTENTION To maintain the correct alignment the screws must be tightened in turn.
Tighten each screw only a small amount before moving to the next screw. Repeat until all
are secure.

d. Close the module flap cover.

Connecting the Detector Gas Tubings to the Manifolds

This sections provides instruction for connecting the detector gas tubings to the
manifolds of the GDI mechanical module and GDI electrical interface.

 To connect the gas tubing block to the manifolds

Figure 514 shows manifolds of the GDI mechanical module and GDI electrical interface.

Note Figure 514, Figure 515, Figure 516, Figure 517, and Figure 522 do not show
the third-party detector for graphic convenience. Suppose that it is installed in the
detector module GDI.

Figure 514. Detector Gas Tubings Manifolds (1)

Third-party
Detector

Detector Gas Tubings

Manifolds

1. Take one, two, or three segments of the 1/16-in.stainless steel tubings (provided)
according to the detector gases required and long enough to properly connect both the
detector gas tubings manifolds.

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 9 Adding Modules
Adding a Generic Detector Interface

2. Connect the detector gas tubings to the manifold of the GDI mechanical module.
See Figure 515.

Figure 515. Detector Gas Tubings Connections

Detector Gases
Tubings
Third-party
Detector Detector Gases
Tubings Manifold

a. Connect the detector gas tubings to the numbered inlet ports using the appropriate
Swagelok® 1/16-in. nut and ferrules. Use a 1/4-in. wrench to tighten the fittings.

ATTENTION The inlet ports of the manifold on the GDI mechanical module are
numbered 1, 2, and 3 respectively. Pay attention to the correct order when you connect
each detector gas tubing to the corresponding outlet ports marked Gas 1, Gas 2, and
Gas 3 on the GDI electrical interface.

Connect the detector gas tubings for the third-party detector as follows:
• Outlet port Gas 1 to inlet port 1
• Outlet port Gas 2 to inlet port 2
• Outlet port Gas 3 = Air (Wall) to inlet port 3

b. Repeat step a until all the required detector gas tubings are connected to the GDI
mechanical module.
c. Bend and run the detector gas tubings along the top cover.

Note The bending of the detector gas tubings shown in Figure 516 and Figure 517 is
indicative.

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9 Adding Modules
Adding a Generic Detector Interface

Figure 516. Bending of the Detector Gas Tubings on the TRACE 1300/1310 GC

Gas Tubings
Third-party
Detector

Figure 517. Bending of the Detector Gas Tubings on the TRACE 1310 Auxiliary Oven

Gas Tubings

Third-party
Detector

3. Connect the relevant detector gas tubings to the manifold of the GDI electrical interface.
See Figure 518.

Figure 518. Detector Gas Tubings Connection to the GDI Electrical Interface - External View (1)

Gas 3

Gas 2

Gas 1

a. Guide the detector gas tubings up to reach the GDI electrical interface.

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 9 Adding Modules
Adding a Generic Detector Interface

b. Bend and run the detector gas tubing along the back panel of the GC (or TRACE
1310 Auxiliary Oven) until its end reaches the corresponding numbered gas outlet
port on the GDI electrical interface.
c. Connect the detector gas tubing to the corresponding numbered outlet port using the
appropriate Swagelok® 1/16-in. nut and ferrules. Use a 1/4-in. wrench to tighten the
fittings.

d. Repeat step b and step c until all the required detector gas tubings are connected to
the GDI electrical interface.
The result of the operation is shown in Figure 519.

Figure 519. Detector Gas Tubings Connection to the GDI Electrical Interface - External View (2)

Gas 3
Gas 2
Gas 1

Note The bending of the gas tubing is indicative.

4. Connect the supply gas to the GDI electrical interface.

Note Use the 1/8-in. Swagelok fittings provided on the gas inlet ports to connect the
gas lines.

a. Connect the gas line to the corresponding inlet port using the appropriate nut and
ferrules. Use a 7/16-in. wrench for tightening the fittings.
b. Repeat step a until all the gas lines are connected to the corresponding inlet port on
the GDI electrical interface. See Figure 520.

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Figure 520. Gas Lines Connection to the GDI Electrical Interface

Gas 3
Gas 2
Gas 1

Gas 2 Gas 3

Gas 1

IMPORTANT The maximum nominal inlet pressure for all the inputs is 1000 kPa
(145 psig). The working inlet pressure range is from 400 kPa (58 psig) to 1000 kPa (
145 psig).

Connecting Heater and Signal Cables

If required by the third-party detector, connect the heater cable, the signal cable, or both.

 To connect the heater and signal cables

1. Using the cable provided connect the 5-pin connector marked Heater on the GDI
electrical interface to the detector connector on the GDI mechanical module.
See Figure 521 and Figure 522.

Figure 521. Heater and Signal Connectors

From Third-party
Detector
To Heater
Connector
on Detector
Module

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 9 Adding Modules
Adding a Generic Detector Interface

Figure 522. Heater Cable Connection

Heater Cable From GDI
Electrical Interface

Third-party
Detector

2. Connect the signals cable from the third-party detector to the connector marked Signal
IN on the GDI electrical interface. See Figure 523.

Figure 523. Signal Connection

Signal Cable from

Third-party Detector

Restarting the GC
 To restart the GC

1. Open the gas supplies.

2. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
3. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.

Performing the Third-party Detector Start-up and Optimization

Refer to the third-party detector manual.

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Configuring and Setting GDI Detector

Configure and enable the GDI mechanical module through the user interface of your GC, or
through the CDS in use. Refer to the TRACE 1300 and TRACE 1310 User Guide.

See the following sequences:

• “Configure and set GDI Detector through the touch screen” on page 490
• “Configure and set GDI Detector through the chromatography data system (CDS)” on
page 490

 Configure and set GDI Detector through the touch screen

1. In the main menu select the Configuration icon, the Configuration menu appears.
Select the Front/Back/Aux L/Aux R GDI detector icon to open the relevant sub-menu.
2. In the main menu select the Instrument control icon. The Instrument Control menu
appears. In the Instrument Control menu, select the Front/Back/Aux L/Aux R GDI
detector icon to open the relevant sub-menu.
3. Set the parameters values as required, then return to main menu.

Note For details refer to Chapter 2 in the TRACE 1300/REACE 1310 User Guide.

 Configure and set GDI Detector through the chromatography data system (CDS)

1. In the Configuration window select the Detectors tab.

2. Select the Detector Type: choose GDI.

3. Click GDI Config...; the GDI Configuration page is visualized.

4. Select the Gas Type used for the detector gases Gas 1, Gas 2, and Gas 3.
Choose one: Air, Hydrogen, Nitrogen, Helium, Argon, or Argon/Methane. Nitrogen is
the default gas.
5. Set the Full-scale flow of the restriction installed for each detector gas. Set a value in the
range 1-1000 mL/min. Default value is 50 mL/min.
6. Select the Max. detector temperature in the range from 0 °C to 450 °C.
The default temperature is 400 °C.
7. Select the ADC full-scale voltage. Choose one: 1 V, 5 V, or 10 V. The default value is 1V.

8. Open the GDI Method Page and set the required parameters.

Note For details refer to Chapter 4 in the TRACE 1300/REACE 1310 User Guide.

Note If the heater is installed but the actual Temperature read back is 0 °C, check the
integrity of the heater and the temperature probe.

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Adding an Analog Output Interface

Adding an Analog Output Interface

This section provides instructions for installing the Analog Output Interface (AOI) on your
TRACE 1300/1310 GC.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To install an analog output interface

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.

4. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
1. Remove the cover of the external modules housing where installing the Analog Output
Interface module. See Figure 524.

Figure 524. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

a. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws, then remove the cover from the housing.
2. Install the Analog Output Interface into the housing.
a. Loosen the two hexagonal screws under the module. See Figure 525.

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 491
 Figure 525. AOI Installation (1)

Hexagonal Screws

b. Carefully place the Analog Output Interface into the left or right housing until the
hexagonal screws couple with the slots on the floor of the GC. See Figure 526.
Figure 526. AOI Installation (2)

Slots

c. Finger-tighten the hexagonal screws slightly, or use a 10-mm wrench.

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the auxiliary module when necessary.
 9 Adding Modules
Adding an Analog Output Interface

The result of the installation is shown in Figure 508.

Figure 527. Analog Output Interface Module Installed into the GC

Cables Holder
AOI Control Module

3. Connect the analog output interface module. See Figure 528.

Figure 528. AOI Connections

Device
Data System Analog
Acquisition Box

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9 Adding Modules
Adding an Analog Output Interface

Tip To avoid the contact with the hot air from the vents, it is suggested to gather the
electrical cables into cables holder.

a. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
b. Connect up to four channels CH-1, CH-2, CH-3, CH-4 to your device selecting the
full scale of each analogue signal from 1 V to 10 V according to your needs.

IMPORTANT Only connect the Ground wire to the GND contact of the AOI module or
of the your device. Do not connect the Ground cable to both the devices.

c. Connect the Start OUT contact closure to Start IN TTL line of the Data system
analog acquisition box.
4. Open the gas supplies.
5. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
6. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
7. Configure and enable the system installed through the touch screen of your GC. Refer to
the TRACE 1300 and TRACE 1310 User Guide.

494 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 10

Adding Systems
This chapter describes how to install any added system that is available for the TRACE
1300/TRACE 1310. See the TRACE 1300 and TRACE 1310 Spare Parts Guide for
information about ordering the equipment in this chapter.

Contents
• Adding the Oven Cryo System
• Adding the PTV and PTVBKF Cryo System
• Adding an Auxiliary Gas System
• Adding the Hydrogen Sensor

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Adding the Oven Cryo System

Adding the Oven Cryo System

This section provides instructions for installing and configuring the Oven Cryo system on
your TRACE 1300/TRACE 1310 using the dedicated kit. See Figure 529.

Figure 529. Oven Cryo System for Carbon Dioxide and Liquid Nitrogen

Oven Cryo System for Carbon Dioxide

Oven Cryo System for Liquid Nitrogen

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Adding the Oven Cryo System

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

The cryo system requires the use of liquid nitrogen or carbon dioxide as coolant. Before
using liquid nitrogen or carbon dioxide, read the indications of hazards, and the
instructions reported in the Safety sheet supplied by the manufacturer with reference to
the CAS number (Chemical Abstract Service). See also “Liquid Nitrogen Safety
Precautions” on page xxix and “Carbon Dioxide Safety Precautions” on page xxx.

Oven Cryo System Overview

Two Oven Cryo Upgrade Kits are available:
• Oven Cryo with Liquid Nitrogen (LN2) as coolant.
• Oven Cryo with Carbon Dioxide (CO2) as coolant.

Each upgrade kit contains all the material required to install the Oven Cryo system on your
GC. See Figure 530 and Figure 531.

Figure 530. Oven Cryo Kit for Carbon Dioxide

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Adding the Oven Cryo System

Figure 531. Oven Cryo Kit for Liquid Nitrogen

Each upgrade kit contains all the material required to install the Oven Cryo system on your
GC:
• Dedicated solenoid valve mounted on a support bracket.
• Tube for the coolant into the oven.
• Coolant tank tube with connection fittings.
• Aux Temperature/Cryo Module that should be installed into a free external module
housing provided on the back of the GC. See Figure 532.

Figure 532. External Modules Housing

External Modules Housing

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Installing the Oven Cryo System

 To install the Oven Cryo System

1. Put the GC in standby condition.

2. Cool the oven, injectors, and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, in the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the Left Side panel.
a. Open the front door of the GC. Using a T20 Torxhead screwdriver, unscrew the left
side panel screw from the interior front panel. See Figure 533.

Figure 533. Left Side Panel Fixing Screw

b. Slide the panel towards the back of the instrument up to the stop.
c. Remove the panel by pulling it outwards. Be aware that the ground wire is attached
to the panel. See Figure 534.

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Figure 534. Left Panel Removal

d. Unplug the ground wire from the panel.

Note Pay attention to the positioning of the ground wire plug, so it can be
reconnected in the same way it was removed.

7. Install the Aux Temperature/Cryo Module. See Figure 535

Figure 535. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

a. Remove the cover of the external modules housing where installing the module.
b. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws.
c. Remove the covers from the housing.
8. Install the module into the housing
a. Loosen the two hexagonal screws under the module. See Figure 536.

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Figure 536. Module Installation (1)

Hexagonal Screws

b. Place the module into the left or right housing until the hexagonal screws couple the
slots on the floor of the GC. See Figure 537.

Figure 537. Module Installation (2)

Slots

c. Finger-tighten the hexagonal screws slightly, or use a 10-mm wrench.

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the auxiliary module when necessary.

The result of the installation is shown in Figure 538.

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Adding the Oven Cryo System

Figure 538. Aux Temperature/Cryo Module Installed into the GC

Cables Holder
Aux Temperature/Cryo Module

9. Install the solenoid valve.

The solenoid valve must be installed in the proper seat on the back of the GC. See
Figure 539.

Figure 539. Solenoid Valve Assembly Seat

a. With care, take the solenoid valve assembly and remove the two screws from the
brackets using a T15 Torxhead screwdriver. These screws are used to fix the bracket
on the GC.
If not already done, connect the coolant tube to the solenoid valve using the proper
nut and ferrule. Use a 7/16-in. wrench for tightening the fittings. See Figure 540.

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Figure 540. Solenoid Valve Assembly

Solenoid Valve Assemble

for Carbon Dioxide

Solenoid Valve Assemble

for Liquid Nitrogen

b. Guide the solenoid valve assembly into its seat on the back of the GC, and the tube
for the coolant into the oven through the holes provided. See Figure 541 and
Figure 542.

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Figure 541. Installation of the Solenoid Valve Assembly for Carbon Dioxide

Figure 542. Installation of Solenoid Valve Assembly for Liquid Nitrogen

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c. Fix the solenoid valve assembly to the back of the GC using the two fixing screws
previously removed. See Figure 543.

Figure 543. Solenoid Valve Assemble Fixing

Solenoid Valve
Assembly
Fixing Screws

10. Connect the cryogenic tank tube to the solenoid valve assembly.
a. Connect the proper end of the cryo supply tube to the 1/8-in. NPT connection of
the solenoid valve using the proper nut and ferrule. Use a 7/16-in. wrench for
tightening the fittings. See Figure 544 and Figure 545.

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Figure 544. Oven Cryo Supply Tube for Carbon Dioxide Connection

Nut Ferrule 1/8-in. NPT

Fittings for the connection to the Liquid Carbon Dioxide Tank

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Figure 545. Oven Cryo Supply Tube for Liquid Nitrogen Connection

Nut Ferrule 1/8-in. NPT

Fittings for the connection to the Liquid Nitrogen Tank

b. Connect the other end to the coolant container using the appropriate nuts and
ferrules.

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11. Connect the cryo valve to the Aux Temperature/Cryo Module.

a. Connect the cable provided to the solenoid valve connector, and guide the cable
through the slot in the centre of the back panel. See Figure 546.

Figure 546. Solenoid Valve Cable Connection

b. Connect the cryo solenoid valve to the 2-pin connector marked Cryo Valves - Oven
using the cable provided. See Figure 547.

Figure 547. Cryo Valves: Oven

c. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
d. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights after the GC is powered on.

Note For further details regarding the installation of the Aux Temperature/Cryo
module, see the section “Adding an Aux Temperature/Cryo Module” on
page 443.

e. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.

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12. Mount the left side panel proceeding in the reverse order in which the left side panel was
removed.
13. Open the gas supplies.
14. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
15. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
16. Configure and enable the cryogenic system through the user interface of your GC, or
through the CDS in use. Refer to the TRACE 1300 and TRACE 1310 User Guide.
a. Configuration and enabling through the touch screen.
i. In the main menu select the Configuration icon. The Configuration menu
appears.
ii. In the Configuration menu, select the Oven icon to open the relevant submenu.
iii. Set the Cryogenic parameters.
– Cryogenic Type — Enable or disable the cryogenic system when it is
installed and configured with Carbon Dioxide or Liquid Nitrogen as a
coolant. Select between LN2, CO2, or none.
– Cryo timeout — Enter the time at which the cryo system will be disabled.
Enter a value from 0–30 min.
– Cryo Threshold — Specify the temperature at which the cryo system begins
to supply the coolant. Enter a value from 40-200 °C.
iv. Return to main menu.
b. Configuration and enabling through the Chromatography Data System.
i. In the Configuration window select the Auxiliary tab.
ii. Select the Auxiliary control module check box to enable the setting for the
auxiliary control of the module option installed on your GC.
iii. Select the Oven cryogenics check box.
iv. Select the Cryo type used by your cryogenic option. Choose one: Liquid
Nitrogen or Carbon Dioxide.
v. Open the Oven page. Select the Cryogenics enable check box to enable the
cryogenic system.
vi. In Cryo threshold text box specify the temperature at which the cryo system
begins to supply the coolant. Enter a value from 40-200 °C.

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Adding the PTV and PTVBKF Cryo System

17. Set the normal detector, injector, and GC working conditions.

Adding the PTV and PTVBKF Cryo System

This section provides instructions for installing and configuring the PTV and PTVBKF Cryo
system on your TRACE 1300/TRACE 1310 using the dedicated kit. See Figure 548 and
Figure 549.

Figure 548. PTV and PTVBKF Single and Double Cryo System for Carbon Dioxide

Figure 549. PTV and PTVBKF Single and Double Cryo System for Liquid Nitrogen

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Adding the PTV and PTVBKF Cryo System

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.
The cryo system requires the use of liquid nitrogen or carbon dioxide as coolant. Before
using liquid nitrogen or carbon dioxide, read the hazard indications and the instructions
reported in the Safety sheet supplied by the manufacturer with reference to the CAS
number (Chemical Abstract Service). See also “Liquid Nitrogen Safety Precautions” on
page xxix and “Carbon Dioxide Safety Precautions” on page xxx.

PTV/PTVBKF Cryo System Overview

Two PTV/PTVBKF Cryo Upgrade Kits are available:
• PTV/PTVBKF Cryo with Liquid Nitrogen (LN2) as coolant.
• PTV/PTVBKF Cryo with Carbon Dioxide (CO2) as coolant.

Each upgrade kit contains all the material required to install the PTV/PTVBKF Cryo system
on your GC. See Figure 550 and Figure 551.

Figure 550. PTV/PTVBKF Cryo Kit for Carbon Dioxide

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Figure 551. PTV/PTVBKF Cryo Kit for Liquid Nitrogen

Each upgrade kit contains all the material required to install the PTV/PTVBKF Cryo system
on your GC:
• Dedicated solenoid valve mounted on a support bracket.
• Tube for the coolant into the PTV/PTVBKF injector module.
• Coolant tank tube with connection fittings.
• Aux Temperature/Cryo Module that should be installed into a free external module
housing provided on the back of the GC. See Figure 552.

Figure 552. External Modules Housing

External Modules Housing

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Installing the PTV/PTVBKF Cryo System

 To install the PTV/PTVBKF Cryo System

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Put the autosampler away if present.
7. Install the Aux Temperature/Cryo Module.
a. Remove the cover of the external modules housing where installing the module. See
Figure 553.

Figure 553. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

b. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws.
c. Move and drive the cover out from the housing.
8. Install the module into the housing
a. Loosen the two hexagonal screws under the module. See Figure 554.

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Figure 554. Module Installation (1)

Hexagonal Screws

b. Place the module into the left or right housing until the hexagonal screws couple with
the slots on the floor of the GC. See Figure 555.

Figure 555. Module Installation (2)

Slots

c. Finger-tighten the hexagonal screws slightly or use a 10-mm wrench.

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the auxiliary module when necessary.

The result of the installation is shown in Figure 556.

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Figure 556. Aux Temperature/Cryo Module Installed into the GC

Cables Holder

Aux Temperature/Cryo Module

9. Install the proper solenoid valve assembly.

The solenoid valve must be installed in the proper seat on the back of the GC.
• In case of a single cryo kit, mount the solenoid assembly as shown in Figure 557.
• In case of double cryo kit, mount the two solenoid valves assembly as shown in
Figure 558.

Figure 557. Single Solenoid Valve Assemble Installation

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Figure 558. Double Solenoid Valve Assemble Installation

10. Insert the coolant tube into the PTV/PTVBKF injector module.
a. Open the module flap cover.
b. On the top of the injector, undo and remove the screw closed to the coolant tube
insertion hole. See Figure 559.

Figure 559. Coolant Tube Insertion Hole

Screw

Insertion Hole

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c. Insert the coolant tube into the insertion hole, and fix it using the screw previously
removed. See Figure 560.

Figure 560. Installation of the Coolant Tubes for Carbon Dioxide and Liquid Nitrogen

Coolant Tube for Carbon Dioxide

Coolant Tube for Liquid Nitrogen

d. Guide the coolant tube along the GC top cover up to reach the solenoid valve
assemble on the back of the GC. Bend the tube if necessary.
11. Connect the coolant tube to the solenoid valve assemble.
a. Connect the coolant tube to the solenoid valve assembly using the proper nut and
ferrule. Use a 7/16-in. wrench for tightening the fittings. See Figure 561.

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Figure 561. Coolant Tube Connection to the Solenoid Valve

Coolant Tube for Carbon Dioxide

Coolant Tube for Liquid Nitrogen

b. Connect the proper end of the cryo supply tube to the solenoid valve using the
proper nut and ferrule. Use a 7/16-in. wrench for tightening the fittings.
See Figure 562.

Figure 562. Cryo Supply Tube Connection to the Solenoid Valve

Cryo Supply Tube for Carbon Dioxide

Cryo Supply Tube for Liquid Nitrogen

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c. Connect the other end of the cryo supply tube to the coolant container using the
appropriate nuts and ferrules. Use a 7/16-in. wrench for tightening the fittings.
12. Connect the cryo valve to the Aux Temperature/Cryo Module.
a. Connect the cryo solenoid valve to the 2-pin connector marked Cryo Valves - Front
Inlet or Cryo Valves-Back Inlet using the cable provided. See Figure 563.

Figure 563. Cryo Valves: Oven

b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights after the GC is powered on.

Note For further details regarding the installation of the Aux Temperature/Cryo
module, see the section “Adding an Aux Temperature/Cryo Module” on
page 443.

d. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
13. Mount the left side panel proceeding in reverse order which the left side panel was
removed.
14. Open the gas supplies.
15. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
16. Power on the GC.
a. Plug the power cable to the AC Input connector on the back of the GC, and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
17. Configure and enable the cryogenic system through the user interface of your GC, or
through the CDS in use. Refer to the TRACE 1300 and TRACE 1310 User Guide.
a. Configuration and enabling through the touch screen.
i. In the main menu select the Configuration icon, the Configuration menu
appears.

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ii. In the Configuration menu, select the Front/Back PTV or PTVBKF icon to
open the relevant submenu.
iii. Set the Cryogenic parameters.
– Cryogenic Type — Enable or disable the cryogenic system when it is
installed and configured with Carbon Dioxide or Liquid Nitrogen as a
coolant. Select between LN2, CO2, none.
– Cryo timeout — Enter the time at which the cryo system will be disabled.
Enter a value from 0–30 min.
– Cryo Threshold — Specify the temperature at which the cryo system begins
to supply the coolant. Enter a value from 40-200 °C.
– Cryo Cool at — Specify the temperature at which the cryogenic system
begins to supply the coolant. Enter a value from 40-200 °C.
iv. Return to main menu.
b. Configuration and enabling through the Chromatography Data System.
i. In the Configuration window select the Auxiliary tab.
ii. Select the Auxiliary control module check box to enable the setting for the
auxiliary control of module and option installed on your GC.
iii. Select the Front inlet cryogenics or/and Back inlet cryogenics check box.
iv. Select the Cryo type used by your cryogenic option. Choose one: Liquid
Nitrogen or Carbon Dioxide.
v. Open the PTV/PTVBKF page. Select the Cryogenics enable check box to
enable the cryogenic system.
vi. In Cool during combo box, select when you want the cooling to be done.
Choose between Prep-Run or Post -Run.
vii. In Cryo threshold text box specify the temperature at which the cryo system
begins to supply the coolant. Enter a value in the range 40-200 °C.
viii. In Cryo timeout text box enter the time at which the cryo system will be
disabled. Enter a value in the range 0–30 min.
18. If present, move the autosampler towards the module to restore the original alignment.
19. Set the normal detector, injector, and GC working conditions.

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Adding an Auxiliary Gas System

This section provides instructions for updating your TRACE 1300/TRACE 1310 with the
Auxiliary Gas System.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

The Auxiliary Gas Module is shipped with a protecting plate screwed on the manifold.
Remove this plate before installing the module. See Installing and Connecting the
Auxiliary Gas Module on page 534 for details.

The auxiliary gas system comprises the auxiliary gas module and the auxiliary gas interface.
See Figure 564.

Figure 564. Auxiliary Gas Module and Interface

Auxiliary Gas Module with Gas Tubing Block

Front View Back View

Auxiliary Gas Interface

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Auxiliary Gas Module Overview

The auxiliary gas module includes the following connections. See Figure 565

Figure 565. Auxiliary Gas Module Connections

1 2 3

1. Switch marked Primary/Secondary used to set two Auxiliary Gas modules

simultaneously present, one as Primary, and the other as Secondary. The primary module
controls the aux pressures from 1 to 3, while the secondary module controls the aux
pressures from 4 to 6. See also the point 3.
2. 15-pin female connectors marked Bus for the communication with the GC.
3. Three inlets ports marked Gas 1 (4), Gas 2 (5), and Gas 3 (6) for the connection up to
three auxiliary carrier gases. If two modules are present, up to six auxiliary carrier gases
can be connected. See also the point 1.

Auxiliary Gas Interface Overview

The auxiliary gas interface is installed and fixed on the left or right wall of the GC oven,
through the ducts provided, for the coupling with a mass spectrometer. See Figure 566.

Note The Auxiliary Gas Interface is fixed on the exterior wall of the GC oven through the
slots provided on the collar duct. Align the slots to the fixing holes accordingly.

Collar Duct
Slots

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Figure 566. Auxiliary Gas Interface Installed in the Oven

Auxiliary Gas Interface

installed on the Left

Auxiliary Gas Interface

installed on the Right

Note If your GC is equipped with the oven for the coupling with a Thermo Scientific
high resolution mass spectrometers (HRMS), the Auxiliary Gas Interface is installed
through the ducts provided on the left and right walls of the oven as well as the GC
equipped with the standard oven. See “Installing the Auxiliary Gas Interface on the Oven
for HRMS” on page 532.

Left Wall Right Wall

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The module should be installed into a free external module housing provided on the back of
the GC. See Figure 567.

Figure 567. External Modules Housing

External Modules Housing

Preliminary Operations
Before starting, the following preliminary operation must be carried out.
1. Put the GC in standby condition.
2. Cool the oven, injectors, and detectors to room temperature.

Note By pressing the Maintenance button, the GC cool down is automatically

carried out.

3. Close the gas supplies.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector on the back of the GC, and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.

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Getting Started
To install and connect the Auxiliary Gas Interface and the Auxiliary Gas Module, see the
following sections:
• “Installing the Auxiliary Gas Interface on the Left Wall of the Oven” on page 525
• “Installing the Auxiliary Gas Interface on the Right Wall of the Oven” on page 528
• “Installing the Auxiliary Gas Interface on the Oven for HRMS” on page 532
• “Installing and Connecting the Auxiliary Gas Module” on page 534

Installing the Auxiliary Gas Interface on the Left Wall of the Oven
 To install the auxiliary gas interface on the left wall of the oven

1. Make sure that the preliminary operations have been carried out. See “Preliminary
Operations” on page 524.
2. Remove the left side panel.
a. Open the front door of the GC.

b. Using a T20 Torxhead screwdriver, unscrew the left side panel screw from the interior
front panel. See Figure 568. Save the screw because it will be reused later.

Figure 568. Left Side Panel Fixing Screw

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c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel pulling it outward being aware that the ground wire is attached to
the panel. See Figure 569.
e. Unplug the ground wire from the panel.

Figure 569. Left Side Panel Removal

3. Prepare the duct for the installation of the auxiliary gas interface.
a. Remove the partial cut shaped plate from the exterior wall of the oven box for
accessing the insulating material. See Figure 570.

Figure 570. Perform the Duct for the Auxiliary Gas Interface (1)

Partial Cut Shaped Plate

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b. Using a knife or similar tool, gently cut the insulating material following the border.
See Figure 571.

Figure 571. Perform the Duct for the Auxiliary Gas Interface (2)

c. Save the removed insulating material in a safe place because it can be reused.
d. On the left side wall in the interior of the oven, remove the partial cut plate from the
corresponding duct. See Figure 572.

Figure 572. Perform the Duct for the Auxiliary Gas Interface (3)

Partial Cut Plate

e. Insert the auxiliary gas interface into the duct. Fix the interface on the exterior wall of
the oven box using the fixing screws provided. See Figure 573.

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Figure 573. Perform the Duct for the Auxiliary Gas Interface (4)

4. Jump to the section “Installing and Connecting the Auxiliary Gas Module” on page 534.

Installing the Auxiliary Gas Interface on the Right Wall of the Oven
 To install the auxiliary gas interface on the right wall of the oven

1. Make sure that the preliminary operations have been carried out. See “Preliminary
Operations” on page 524.
2. Remove the right side panel.
a. Open the front door of the GC.

b. Using a T20 Torxhead screwdriver, unscrew the left side panel screw from the interior
front panel. See Figure 574. Save the screw because it will be reused later.

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Figure 574. Right Side Panel Fixing Screw

c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel pulling it outward. Be aware that the ground wire is attached to the
panel. See Figure 575.
e. Unplug the ground wire from the panel.

Figure 575. Right Side Panel Removal

3. Prepare the duct for the installation of the auxiliary gas interface.

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a. Remove the partial cut shaped plate from the exterior wall of the oven box for
accessing the insulating material. See Figure 576.

Figure 576. Perform the Duct for the Auxiliary Gas Interface (1)

Partial Cut Shaped Plate

b. Using a knife or similar tool, gently cut the insulating material following the border.
See Figure 577.

Figure 577. Perform the Duct for the Auxiliary Gas Interface (2)

c. Save the removed insulating material in a safe place because it can be reused.
d. On the left side wall in the interior of the oven, remove the partial cut plate from the
corresponding duct. See Figure 578.

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Figure 578. Perform the Duct for the Auxiliary Gas Interface (3)

Partial Cut Plate

e. Insert the auxiliary gas interface into the duct. Fix the interface on the exterior wall of
the oven box using the fixing screws provided. See Figure 579.

Figure 579. Perform the Duct for the Auxiliary Gas Interface (4)

4. Jump to the section “Installing and Connecting the Auxiliary Gas Module” on page 534.

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Installing the Auxiliary Gas Interface on the Oven for HRMS

 To make the duct into the oven for HRMS

1. Remove the left/right side panel.

a. Open the front door of the GC.
b. Use a T20 Torxhead screwdriver to loosen the left/right side panel screw from the
interior front panel. Save the screw because it will be reused later.
c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel pulling it outward being aware that the ground wire is attached to
the panel.
2. Prepare the duct for the installation of the auxiliary gas interface.
a. On the left/right exterior wall of the oven box, remove the partial cut plate of the
duct of interest.
b. Remove the pre-shaped plug of insulating material from the duct provided. See
Figure 580.

Figure 580. Left/Right Exterior Oven Wall Box View

Left Oven Wall Right Oven Wall

IMPORTANT Save the pre-shaped plug of insulating material in a safe place because it
could be reused.

c. On the interior of the oven box, remove the partial cut plate from the corresponding
duct. See Figure 581.

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Adding an Auxiliary Gas System

Figure 581. Left/Right Interior Oven Wall Box View

Left Oven Wall

Right Oven Wall

3. Insert the auxiliary gas interface into the duct. Fix the interface on the exterior wall of the
oven box using the fixing screws provided.
4. Jump to the section “Installing and Connecting the Auxiliary Gas Module” on page 534.

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Adding an Auxiliary Gas System

Installing and Connecting the Auxiliary Gas Module

 To install and connect the Auxiliary Gas Module

1. Remove the cover of the external modules housing where installing the module. See
Figure 582.

Figure 582. Housing Cover Removal

Left Cover
Screws

Right Cover
Screws

Left/Right Housing Covers

a. Using a T20 Torxhead screwdriver, unscrew and remove the left or right housing
cover screws.
b. Remove the cover from the housing.
2. Remove the manifold protecting plate
a. Using a T20 Torxhead screwdriver, unscrew the two fixing screws, and remove the
protecting plate from the manifold. Save the protecting plate and the fixing screws.

Figure 583. Manifold Protecting Plate

Protecting Plate

Fixing Screws

3. Install the module into the housing

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Adding an Auxiliary Gas System

a. Loosen the two hexagonal screws under the module. See Figure 584.

Figure 584. Module Installation (1)

Hexagonal Screws

b. Carefully place the module into the left or right housing.

c. Push the module until the hexagonal screws couple with the slots on the floor of the
GC. See Figure 585.

Figure 585. Module Installation (2)

Slots

d. Finger-tighten the hexagonal screws slightly, or use a 10-mm wrench.

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Adding an Auxiliary Gas System

Note Always keep the hexagonal screws in their place. This allows you an easier
removal of the auxiliary module when necessary.

The result of the installation is shown in Figure 586.

Figure 586. Auxiliary Gas Module Installed into the GC

Cables Holder
Auxiliary Gas Module

4. Connect the gas tubing block to the manifold.

Figure 587 shows the gas tubing block and the manifold located into the auxiliary gas
module.

Figure 587. Gas Tubing Block and Manifold

Gas Tubing Block

Fixing Screws

Manifold

Fixing Holes

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a. Carefully guide the gas tubing block on the manifold located into the auxiliary gas
module. See Figure 588 if the module is installed on the right, or Figure 589 if the
module is installed on the left.

Figure 588. Installation on the Right Side

Figure 589. Installation on the Left Side

b. Align the fixing screws of the gas tubing block with the corresponding holes on the
manifold.
c. Use the T20 Torxhead screwdriver to tighten the two fixing screws without
overtightening.
5. Connect the gas tubes of interest to the Auxiliary Gas Interface.
a. Guide the three gas tubes up to reach the Auxiliary Gas interface.

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Adding an Auxiliary Gas System

Note The length of the tubes allows them to reach the Auxiliary Gas Interface whether
they are installed on the same side or on the opposite side of the Auxiliary Gas Module.

ATTENTION The three gas tubes, coming from the gas tubing block, are numbered 1, 2,
and 3 respectively. Pay attention to the correct order when you connect each tube to the
corresponding inlet on the auxiliary gas interface. The end of each tube is provided with a
label indicating the type of gas.

b. Bend the gas tube until its end reaches the corresponding numbered inlet port of the
auxiliary interface.
c. Connect the gas tube to the corresponding numbered inlet port using the appropriate
nut and ferrules. Use a 7/16-in. wrench to tighten the fittings. See Figure 590.

Figure 590. Gas Tubes Connection to the Auxiliary Gas Interface - External View(

d. Repeat step b and step c until all the gas tubes of interest are connected to the
auxiliary gas interface.
e. In the GC oven, carry out the connections of the components of interest to the
corresponding inlet ports of the auxiliary gas interface using the appropriate nut and
ferrules.

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Adding an Auxiliary Gas System

f. Repeat step e until all the components are connected to the auxiliary gas interface.
See Figure 591.
Figure 591. Gas Tubes Connection to the Auxiliary Gas Interface - Internal View(

6. Connect the Supply Gas to the Auxiliary Gas Module.

Note Use the 1/8-in. Swagelok fittings provided on the gas inlet ports to connect the
gas lines.

a. Connect the gas line to the corresponding inlet port of interest using the appropriate
nut and ferrules. Use a 7/16-in. wrench for tightening the fittings.
b. Repeat step a until all the gas lines of interest are connected to the corresponding
inlet port on the auxiliary gas module. See Figure 592.

Figure 592. Gas Line Connection to the Auxiliary Gas Interface

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Adding an Auxiliary Gas System

IMPORTANT The maximum nominal inlet pressure for all the inputs is 1000 kPa
(145 psig). The working inlet pressure range is from 400 kPa (58 psig) to 1000 kPa
(145 psig).

7. Connect the Auxiliary Gas Module electrically.

a. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.

IMPORTANT If two Auxiliary Gas modules are simultaneously present, one must be set as
Primary and the other as Secondary by mean of dedicated switch on the back panel.
See the example below.

Secondary

Primary

The primary module controls the aux pressures from 1 to 3, while the secondary module
controls the aux pressures from 4 to 6.

8. Replace the left/right side panel.

a. Plug the ground wire previously removed into the left/right panel.
b. Place the left/right panel and attach the screw holding it in place.
9. Open the gas supplies.
10. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
11. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.

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12. Configure and enable the Auxiliary Gas system through the user interface of your GC, or
through the CDS in use. Refer to the TRACE 1300 and TRACE 1310 User Guide.
a. Configuration and enabling through the touch screen.
i. In the main menu select the Instrument control icon. The Instrument Control
menu appears.
ii. In the Instrument Control menu, select the Auxiliary icon to open the relevant
submenu.

iii. Set the Aux Gas Pressure values as required, then return to main menu.
b. Configuration and enabling through the Chromatography Data System.
i. In the Configuration window select the Auxiliary tab.
ii. Select the check box Auxiliary Carrier Module 1/2 according to the auxiliary
carrier module installed on your GC.
iii. Select the Auxiliary Pressure check box to enable up to six auxiliary Pressures,
and the adjacent field.
iv. According the inlet ports connected to the Auxiliary Gas Interface, select the
corresponding check box and set the pressure in the adjacent field.
13. Set the normal detector, injector, and GC working conditions.

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10 Adding Systems
Adding the Hydrogen Sensor

Adding the Hydrogen Sensor

This section provides instructions for updating your TRACE 1300/TRACE 1310 with the
hydrogen sensor. See Figure 593.

Figure 593. Hydrogen Sensor

Fixing Screw

Sensor Cable
Sensor Body
Sensor Tube

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 10 Adding Systems
Adding the Hydrogen Sensor

 To add the hydrogen sensor

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

IMPORTANT The hydrogen sensor requires a GC firmware version 1.03 or later.

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.
3. Close the gas supplies.

4. Power off the GC.

a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.
6. Remove the left side panel.
a. Open the front door of the GC.

b. Using a T20 Torxhead screwdriver, unscrew the left side panel screw from the interior
front panel. Save the screw because it will be reused later.

c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel by pulling it outward. Be aware that the ground wire is attached to
the panel.
e. Unplug the ground wire from the panel.

7. Remove the back cover.

a. Use a T20 Torxhead screwdriver to remove the four screws that secure the back cover
to the GC.

b. Lift the cover off using the cover handle. Be aware that the ground wire is attached to
the back panel.

Note Pay attention to the positioning of the ground wire plug, so it can be reconnected in
the same way it was removed.

8. Perforate the duct for the installation of the sensor into the oven.
a. Looking the interior of the GC from the back side, locate the duck provided on the
back wall of the oven. See Figure 594.

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Adding the Hydrogen Sensor

Figure 594. Duck for Hydrogen Sensor

Duct for Hydrogen Sensor

b. Using a tool (for example a punch), perforate the insulating material until the tool
protrudes into the oven. Make sure the duct is free of insulating material.
c. Insert the sensor tube adapter into the duct and fix the adapter using the screw
provided. See Figure 595.

Figure 595. Sensor Tube Adapter Installation (1)

Sensor Tube Adapter Fixing Screw

Sensor Tube Adapter

9. Open the front door.

a. Look into oven the duct for the hydrogen sensor previously done. See Figure 596.

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Adding the Hydrogen Sensor

Figure 596. View of the Sensor Tube Duct Into the Oven

Sensor Tube Duct

b. Check the duct is free of insulating material; if not, remove it.

IMPORTANT The duct must be free of the insulating material. If not, it could obstruct
the tube sensor.

10. Install the hydrogen sensor.

a. Move and guide the sensor tube into the duct for the hydrogen sensor. See
Figure 597.

Figure 597. Hydrogen Sensor Installation (1)

Fixing Holes

Sensor Tube

b. Place the hydrogen sensor into the back of the GC aligning the fixing holes to the
corresponding holes on the GC chassis. See Figure 598.

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Adding the Hydrogen Sensor

Figure 598. Hydrogen Sensor Installation (2)

Fixing Screws

c. Fix the hydrogen sensor using the two fixing screws. See Figure 599.

Figure 599. Hydrogen Sensor Installation (3)

d. Look into oven the hydrogen sensor tube that protrudes into the oven. See
Figure 600.

Figure 600. Sensor Tube Protruding Into the Oven

Sensor Tube

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11. Connect the sensor cable to the 10-pin connector marked J8 Hydrogen Sensor of the
Backplane board.
12. Close the oven door.
13. Reinstall the back cover.
a. Reconnect the ground wire to the back cover terminal.
b. Replace the cover proceeding in the reverse order in which it was removed.
14. Reinstall the left side panel.
a. Plug the ground wire to the panel.
b. Reinstall the panel proceeding in the reverse order in which it was removed.
15. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
16. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
17. Set the normal injector, detector and GC working conditions.

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 11

Upgrade Equipment
This chapter describes how to upgrade the TRACE 1300/TRACE 1310. See the TRACE
1300 and TRACE 1310 Spare Parts Guide for information about ordering the equipment in
this chapter.

Contents
• Upgrading a TRACE 1300 to a TRACE 1310
• Upgrading a Stand Alone TRACE 1300/TRACE 1310 to MS Version
• Updating HMI Software From USB Stick

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Upgrading a TRACE 1300 to a TRACE 1310

Upgrading a TRACE 1300 to a TRACE 1310

This section provides the instruction to upgrade a TRACE 1300 to a TRACE 1310 GC.
.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

 To upgrade a TRACE 1300 to a TRACE 1310 GC

1. Put the GC in standby condition.

2. Cool the oven, injectors and detectors to room temperature.
3. Close the gas supplies.
4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
6. Remove the door cover of the TRACE 1300.
a. Open the front door and look for the fixing screws that secure the cover and the
handle to the door. See Figure 601.

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Figure 601. Front Door Back View

Upper Fixing Screws

Door Handle Fixing

Screw

Lower Fixing Screws

Spacer

b. Use a T20 Torxhead screwdriver to remove the screw that secure the handle to the
front door.
c. Pull the door handle out from the front door. See Figure 602.
Save the handle because it must be re-used.

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Figure 602. Door Handle Removal

Door Handle

d. Use a T20 Torxhead screwdriver to remove the three upper and the three lower screws
that secure the cover to the front door. See Figure 603.
Note The lower screw on the lower right corner is screwed into a spacer.

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Figure 603. Door Cover Fixing Screws Removal

Upper Fixing Screws

Lower Fixing Screws

e. Carefully pull the door cover off (see Figure 604), paying attention to the cables that
connect the status panel to the internal section of the door.

Figure 604. Front Door Removal

Door Cover

Front Door

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Upgrading a TRACE 1300 to a TRACE 1310

f. Disconnect the flat cables from the connector located on the front of the door. See
Figure 605.

Figure 605. Cable Removal

Flat Cables

7. Install the door cover of the TRACE 1310.

a. The door cover of the TRACE 1310 includes the touch screen and the cables for its
connection. Figure 606 shows the connection points on the internal section of the
door where the touch screen and ground cables must be connected.

Figure 606. Cables Connections Points

Touch Screen Connection

Point

Ground Connection
Point

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b. Connect the touch screen and ground cables to the proper connection points located
on the front of the door. See Figure 607.

Figure 607. Cables Connections

8. Mount the TRACE 1310 cover door proceeding in the reverse order in which the
TRACE 1300 cover door was removed.
9. Remount the door handle.
10. Open the gas supplies.
11. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
13. Configure the system.
14. Set the normal detector, injector, and GC working conditions.

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Upgrading a Stand Alone TRACE 1300/TRACE 1310 to MS Version

Upgrading a Stand Alone TRACE 1300/TRACE 1310 to MS Version

This section provides the instructions for updating your TRACE 1300/TRACE 1310 stand
alone version to a MS version. According to Thermo Scientific you need a dedicated upgrade
kit to couple the GC with an ISQ Series, TSQ 8000 Series, DSQ II, ITQ, or TSQ Quantum
mass spectrometer.

WARNING This operation must be carried out by authorized and trained Thermo Fisher
Scientific Service Field Engineers.

Preliminary Operations
Before starting, the following preliminary operation must be carried out.
1. Put the GC in standby condition.
2. Cool the oven, injectors and detectors to room temperature.
3. Close the gas supplies.
4. Power off the GC.
a. Push down the power switch (breaker), located at the back of the instrument, to the
position O.
b. Unplug the power cable from the AC Input connector into the back of the GC and
from the wall outlet.
5. If external modules are present, unplug the power cable from the AC Input connector of
each external module, and from the wall outlet.

Getting Started
Depending on your mass spectrometer, see the following sections:
• Coupling with the ISQ Series / TSQ 8000 Series Mass Spectrometer
• Coupling with the DSQ II Mass Spectrometer
• Coupling with the ITQ Mass Spectrometer
• Coupling with the TSQ Quantum Mass Spectrometer

IMPORTANT The temperature control for the DSQ II, ITQ and TSQ Quantum transfer
line is carried out through the Aux Temperature/Cryo Module. See the section “Adding
an Aux Temperature/Cryo Module” on page 443 for details.

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CAUTION - INSTRUMENT DAMAGE: Condition the column before connecting it to the

transfer line. The material released from the column (column bleed) during conditioning
may contaminate the detector.

Coupling with the ISQ Series / TSQ 8000 Series Mass Spectrometer
 To update the GC for the coupling with the ISQ Series /TSQ 8000 Series mass
spectrometer
1. Make sure that the preliminary operations have been carried out. See “Preliminary
Operations” on page 556.
2. Remove the left side panel.
a. Open the front door of the GC.

b. Use a T20 Torxhead screwdriver to screw the left side panel screw from the interior
front panel. See Figure 608. Save the screw because it will be reused later.

Figure 608. Left Side Panel Fixing Screw

c. Slide the panel towards the back of the instrument up to the stop.
d. Remove the panel pulling it outward being aware that the ground wire is attached to
the panel. See Figure 609.
e. Unplug the ground wire from the panel.

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Upgrading a Stand Alone TRACE 1300/TRACE 1310 to MS Version

Figure 609. Left Side Panel Removal

3. Prepare the duct for the transfer line inner tube.

a. Remove the partial cut shaped plate from the exterior wall of the oven box to access
the insulating material. See Figure 610.

Figure 610. Perform the Duct for ISQ Series / TSQ 8000 Series (1)

Partial Cut Shaped Plate

b. Draw up the duct aligning the slot to the fixing holes on the exterior oven wall and
push the duct against the insulating material up to obtain a trace. See Figure 611.

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Figure 611. Perform the Duct for ISQ Series / TSQ 8000 Series (2)

Duct for ISQ Series /TSQ 8000

Series

c. Using a knife or similar tool, gently cut the insulating material following the track.
d. Save the removed insulating material in a safe place because it can be reused.
e. Place the duct and fix it on the exterior wall of the oven box using the fixing screws
provided. See Figure 612.

Figure 612. Perform the Duct for ISQ Series / TSQ 8000 Series (3)

f. On the left side wall in the interior of the oven, remove the partial cut plate from the
corresponding duct. See Figure 613.

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Figure 613. Perform the Duct for ISQ Series / TSQ 8000 Series (4)

Duct for ISQ Series and

TS 8000 Series Partial Cut Plate

4. Replace the left side panel with the left panel for MS provided.
a. Remove the partial cut plate on the GC left panel for MS. See Figure 614.

Figure 614. Left Panel for MS

Duct for ISQ Series and

TS 8000 Series Partial Cut Plate

b. Plug the ground wire previously removed to the left panel for MS provided.
c. Place the left panel for MS and attach the screw holding it in place.
5. Introduce the ISQ Series / TSQ 8000 Series transfer line inner tube into the oven
through the duct provided.
6. Attach the transfer line to the GC column using the proper nut and ferrule.

Note The column must be conditioned before installing into the transfer line. See
“Installing the Column the First Time” on page 57

7. Close the front door of the GC.

8. If external modules are present, plug the power cable to the AC Input connector of each
external module, and to the wall outlet.
9. Power on the GC.

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a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
10. Tune the ISQ Series / TSQ 8000 Series and set its working conditions. Set the GC
working conditions accordingly.
For details please refer to the TRACE 1300 and TRACE 1310 User Guide, and to the ISQ
or TSQ 8000 Series User and Hardware manuals.

Coupling with the DSQ II Mass Spectrometer

 To update the GC for the coupling with the DSQ II mass spectrometer

1. Make sure that the preliminary operations have been carried out. See “Preliminary
Operations” on page 556.
2. Remove the left side panel.
a. Open the front door of the GC.

b. Use a T20 Torxhead screwdriver to screw the left side panel screw from the interior
front panel. See Figure 615. Save the screw because it will be reused later.

Figure 615. Left Side Panel Fixing Screw

c. Slide the panel towards the back of the instrument up to the stop.

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d. Remove the panel pulling it outward being aware that the ground wire is attached to
the panel. See Figure 616.
e. Unplug the ground wire from the panel.

Figure 616. Left Side Panel Removal

3. Prepare the duct for the transfer line inner tube.

a. Remove the partial cut shaped plate from the stirrer wall of the oven box to access the
insulating material. See Figure 617.

Figure 617. Perform the Duct for DSQ II (1)

Partial Cut Shaped Plate

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b. Draw up the duct aligning the slot to the fixing holes on the exterior oven wall and
push the duct against the insulating material up to obtain a track. See Figure 618.

Figure 618. Perform the Duct for DSQ II (2)

Duct for DSQ II

c. Using a knife or similar tool, gently cut the insulating material following the track.
d. Save the removed insulating material in a safe place because it can be reused.
e. Place the duct and fix it on the exterior wall of the oven box using the fixing screws
provided. See Figure 619.

Figure 619. Perform the Duct for DSQ II (3)

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f. On the left side wall in the interior of the oven, remove the partial cut plate from the
corresponding duct. See Figure 620.

Figure 620. Perform the Duct for DSQ II (4)

Duct for DSQ II Partial Cut Plate

4. Replace the left side panel with the left panel for MS provided.
a. Remove the partial cut plate on the GC left panel for MS. See Figure 621.

Figure 621. Left Panel for MS

Duct for DSQ II Partial Cut Plate

b. Plug the ground wire previously removed to the left panel for MS provided.
c. Place the left panel for MS and attach the screw holding it in place.
5. Introduce the DSQ II transfer line inner tube into the oven through the duct provided.

6. Attach the transfer line to the GC column using the proper nut and ferrule.

Note The column must be conditioned before installing into the transfer line. See
“Installing the Column the First Time” on page 57.

7. Place the Aux Temperature/Cryo module into the housing on the back of the GC.
See the section “Adding an Aux Temperature/Cryo Module” on page 443 for details.

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Aux Temperature/Cryo Module

8. Make the electrical connections. See Figure 622.

Figure 622. Electrical Connections

Transfer Line

Cables Holder

a. Connect the heater cable coming from the transfer line to the connector marked
Heater 1 or Heater 2 on the front of the module
b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
Tip To avoid the contact with the hot air from the vents, it is suggested to gather
the electrical cables into the cables holder.

c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights.
9. Close the front door of the GC.

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10. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
11. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
12. Tune the DSQ II and set its working conditions. Set the GC working conditions
accordingly. For details please refer to the TRACE 1300 and TRACE 1310 User Guide and
to the DSQ II User and Hardware manuals.

Coupling with the ITQ Mass Spectrometer

 To update the GC for the coupling with the ITQ mass spectrometer

1. Make sure that the preliminary operations have been carried out. See “Preliminary
Operations” on page 556.
2. Remove the left side panel.
a. Open the front door of the GC.

b. Using a T20 Torxhead screwdriver, unscrew the left side panel screw from the interior
front panel. See Figure 623. Save the screw because it will be reused later.

Figure 623. Left Side Panel Fixing Screw

c. Slide the panel towards the back of the instrument up to the stop.

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d. Remove the panel pulling it outward being aware that the ground wire is attached to
the panel. See Figure 624.
e. Unplug the ground wire from the panel.

Figure 624. Left Side Panel Removal

3. Prepare the duct for the transfer line inner tube.

a. Remove the partial cut shaped plate from the exterior wall of the oven box to access
the insulating material. See Figure 625.

Figure 625. Perform the Duct for ITQ (1)

Partial Cut Shaped Plate

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b. Draw up the duct aligning the slot to the fixing holes on the exterior oven wall and
push the duct against the insulating material up to obtain a track. See Figure 626.

Figure 626. Perform the Duct for ITQ (2)

Duct for ITQ

c. Using a knife or similar tool, gently cut the insulating material following the track.
d. Save the removed insulating material in a safe place because it can be reused.
e. Place the duct and fix it on the exterior wall of the oven box using the fixing screws
provided. See Figure 627.

Figure 627. Perform the Duct for ITQ (3)

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f. On the left side wall in the interior of the oven, remove the partial cut plate from the
corresponding duct. See Figure 628.

Figure 628. Perform the Duct for ITQ (4)

Duct for ITQ Partial Cut Plate

4. Replace the left side panel with the left panel for MS provided.
a. Remove the partial cut plate on the GC left panel for MS. See Figure 629.

Figure 629. Left Panel for MS

Duct for ITQ Partial Cut Plate

b. Plug the ground wire previously removed to the left panel provided for MS.
c. Place the left panel for MS and attach the screw holding it in place.
5. Introduce the ITQ transfer line inner tube into the oven through the duct provided.
6. Attach the transfer line to the GC column using the proper nut and ferrule.

Note The column must be conditioned before installing into the transfer line. See
“Installing the Column the First Time” on page 57.

7. Place the Aux Temperature/Cryo module into the housing on the back of the GC.
See the section “Adding an Aux Temperature/Cryo Module” on page 443 for details.

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Aux Temperature/Cryo Module

8. Making the electrical connections. See Figure 630.

Figure 630. Electrical Connections

Transfer Line

Cables Holder

a. Connect the heater cable coming from the transfer line to the connector marked
Heater 1 or Heater 2 on the front of the module.

ATTENTION An extension cable transfer line is needed when connecting an ITQ mass
spectrometer to the Aux Temperature/Cryo module.

b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.

Tip To avoid the contact with the hot air from the vents, it is suggested to gather
the electrical cables into the cables holder.

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c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights.
9. Close the front door of the GC.
10. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
11. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
12. Tune the ITQ and set its working conditions. Set the GC working conditions
accordingly.
For details please refer to the TRACE 1300 and TRACE 1310 User Guide and to the ITQ
User and Hardware manuals.

Coupling with the TSQ Quantum Mass Spectrometer

 To configure the GC for the coupling with the TSQ Quantum mass spectrometer

1. Make sure that the preliminary operations have been carried out. See “Preliminary
Operations” on page 556.
2. Remove the right side panel.
a. Open the front door of the GC.

b. Using a T20 Torxhead screwdriver, unscrew the left side panel screw from the interior
front panel. See Figure 631. Save the screw because it will be reused later.

Figure 631. Right Side Panel Fixing Screw

c. Slide the panel towards the back of the instrument up to the stop.

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d. Remove the panel pulling it outward being aware that the ground wire is attached to
the panel. See Figure 632.
e. Unplug the ground wire from the panel.

Figure 632. Right Side Panel Removal

3. Prepare the duct for the transfer line inner tube.

a. Remove the partial cut shaped plate from the exterior wall of the oven box to access
the insulating material. See Figure 633.

Figure 633. Perform the Duct for TSQ Quantum (1)

Partial Cut Shaped Plate

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b. Draw up the duct aligning the slot to the fixing holes on the exterior oven wall and
push the duct against the insulating material up to obtain a track. See Figure 634.

Figure 634. Perform the Duct for TSQ Quantum (2)

Duct for TSQ Quantum

c. Using a knife or similar tool, gently cut the insulating material following the track.
d. Save the removed insulating material in a safe place because it can be reused.
e. Place the duct and fix it on the exterior wall of the oven box using the fixing screws
provided. See Figure 635.

Figure 635. Perform the Duct for TSQ Quantum (3)

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f. On the right side wall in the interior of the oven, remove the partial cut plate from
the corresponding duct. See Figure 636.

Figure 636. Perform the Duct for TSQ Quantum (4)

Duct for TSQ Quantum Partial Cut Plate

4. Replace the right side panel with the right panel provided for MS.
a. Using a T20 Torxhead screwdriver, unscrew the duct plate screws from the right
panel for MS. See Figure 637.

Figure 637. Right Panel for MS

Duct Plate

b. Plug the ground wire previously removed to the left panel of MS provided.
c. Place the right panel for MS and attach the screw holding it in place.
5. Introduce the TSQ Quantum transfer line inner tube into the oven through the duct
provided.
6. Attach the transfer line to the GC column using the proper nut and ferrule.

Note The column must be conditioned before installing into the transfer line.
See “Installing the Column the First Time” on page 57.

7. Place the Aux Temperature/Cryo module into the housing on the back of the GC.
See the section “Adding an Aux Temperature/Cryo Module” on page 443 for details.

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Aux Temperature/Cryo Module

8. Making the electrical connections. See Figure 638.

Figure 638. Electrical Connections

Transfer Line

Cables Holder

a. Connect the heater cable coming from the transfer line to the connector marked
Heater 1 or Heater 2 on the front of the module
b. Using the cable provided, connect the 15-pin female connector marked GC Bus on
the module to a Bus interface on the back of the GC.
Tip To avoid the contact with the hot air from the vents, it is suggested to gather
the electrical cables into the cables holder.

c. Plug the power cable to the AC Input connector on the front of the module and to
the wall outlet. The LED marked On lights up.

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9. Close the front door of the GC.

10. Open the gas supplies.
11. If other external modules are present, plug the power cable to the AC Input connector of
each external module, and to the wall outlet.
12. Power on the GC.
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker), located at the back of the instrument, to the
position I.
13. Configure and enable the cryogenic system through the user interface of your GC, or
through the CDS in use. Refer to the TRACE 1300 and TRACE 1310 User Guide.
a. Configuration and enabling through the touch screen.
i. In the main menu select the Instrument control icon, the Instrument Control
menu appears.
ii. In the Instrument Control menu, select the Auxiliary icon to open the relevant
submenu.

iii. Set the Aux Temp 1/2 values as required, then return to main menu.
b. Configuration and enabling through the Chromatography Data System.
i. In the Configuration window select the Auxiliary tab.
ii. Select the Auxiliary control module check box to enable the setting for the
auxiliary control of module and option installed on your GC.
iii. In the Heater 1/Heater 2 combo box specify the present heater control.
iv. Open the Auxiliary setup page.
v. Select Heater 1 and/or Heater 2 check box to enable the relevant heater control.
In the adjacent field set the required temperature.
14. Set the normal detector, injector and GC working conditions.
15. Tune the TSQ Quantum and set its working conditions. Set the GC working conditions
accordingly.
For details please refer to the TRACE 1300 and TRACE 1310 User Guide and to the TSQ
Quantum User and Hardware manuals.

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 11 Upgrade Equipment
Updating HMI Software From USB Stick

Updating HMI Software From USB Stick

This section provides the instruction for updating the HMI software of the TRACE 1310
touch screen through an USB stick.

ATTENTION The upgrade must be performed by authorized and trained Thermo Fisher
Scientific technical personnel.

 To update the HMI Software

1. Copy on your PC the HMI SW XX.XX.XX.ZIP folder of the last HMI software version
received from the Thermo Fisher Scientific GC-GC/MS Custom Support.
2. Unzip the folder for extracting the files contained therein. Figure 639 shows an example
of the files contained into the unzipped folder.

Figure 639. Example of the files content into the HMI software folder

ATTENTION The Hermes_KeyB.exe file and the Languages folder are the fundamental
components always present in the list. The other files shown in the list might change from a
software version and the next.

3. Copy all the files in the root of the USB stick.

4. Insert the USB stick into the USB port positioned below the touch screen.
On the touch screen main menu, press the Configuration icon to open the
Configuration menu.
5. Press the Touch screen icon to open the relevant menu. See Figure 640.

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11 Upgrade Equipment
Updating HMI Software From USB Stick

Figure 640. Touch Screen Main Menu: Configuration

6. The icon Update software from USB appears in the configuration page.
See Figure 641.

Figure 641. USB stick positioned into the USB port below the touch screen

USB Stick

Note It can take a few moments for USB stick to be recognized by the TRACE 1310.
If you do not see the icon Update software from USB in the menu, then return to the
Home screen, and re-enter the Touch screen portion of the Configuration menu.

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Updating HMI Software From USB Stick

7. Press the icon , the following page is visualized:

8. Press the icon Update to start the updating process.

WARNING DO NOT REMOVE THE USB STICK DURING THE UPDATING PROCESS,
THIS COULD DAMAGE THE INSTRUMENT.

9.At the end of the updating process, the program restarts.

10. Only now remove the USB stick from the USB port.

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 12

Troubleshooting
In this chapter, we describe the symptoms of and remedy for each known issue with the
TRACE 1300/TRACE 1310 gas chromatograph. All of these issues are related to hardware,
but your instrument or software will alert you to them. For issues that you discover while
reviewing your data, see the Analytical Troubleshooting section of the TRACE 1300 and
TRACE 1310 User Guide.

Contents
• Investigating Power Supply Issues
• Investigating Communication Issues
• Investigating Sensitivity Issues
• Error Messages
• Contacting Technical Support

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12 Troubleshooting
Investigating Power Supply Issues

Investigating Power Supply Issues

Verify the correct power supply to the instrument.

TRACE 1300/TRACE 1310 will not power-on

Possible Remedies
Make sure the TRACE 1300/TRACE 1310 power cable is properly connected to the instrument and to the correct 220/120 V main power
line outlet.
Verify that the electrical outlet is functioning properly.
Power supply system is faulty. Contact your local Thermo Fisher Scientific customer support organization.

Investigating Communication Issues

Verify the instruments is communicating with the computer.

Software is not communicating with the TRACE 1300/TRACE 1310

Possible Remedies
Make sure the LAN cable is properly connected to the GC.
Confirm the TCP/IP configuration on the computer matches the GC.
Restart the GC.

TRACE 1300/TRACE 1310 does not start or is not ready

Possible Remedies
Verify the GC methods and configuration.
Make sure the electrical connections have been properly carried out.
Confirm the GC handshaking parameters are set properly.

Cannot download methods to the TRACE 1300/TRACE 1310

Possible Remedies
Verify that your instruments are properly configured.

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 12 Troubleshooting
Investigating Sensitivity Issues

Sample data are not acquired

Possible Remedies
Make sure the autosampler methods and configuration include starting up and injecting a sample.
You should also make sure the sample has been injected.
Make sure the cables between autosampler and GC are properly connected.
Add more disk space to the computer if necessary.

GC is not communicating with the PC

Possible Remedies
Make sure the GC is powered on.
Make sure the GC is properly configured.

Autosampler is not communicating with the PC

Possible Remedies
Make sure the autosampler is powered on.
Make sure the autosampler is properly configured.
Make sure the cable between the autosampler and PC is properly connected.

Investigating Sensitivity Issues

Sensitivity issues are usually the result of an air leak, dirty components, or contamination.
Sometimes sensitivity issues can be caused by simple problems such as the carrier gas tank
running out or a sample not being injected into the GC.

If the problem is more complex, then check for air leaks or dirty components. You can prevent
these problems by properly cleaning and maintaining your GC system.

It is normal to see a decrease in sensitivity in the first few injections on a clean system. Before
troubleshooting for sensitivity issues, look for simple solutions, such as fixing a clogged
autosampler syringe or raising the level of your sample.

Poor sensitivity or sudden loss in sensitivity

Possible Remedies
Check the system for leaks and address them.
Clean or replace the GC injection port liner to remove possible contamination, trim the injector end of the column, or replace the septum.

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12 Troubleshooting
Error Messages

Error Messages
Error messages are visualized in case of GC malfunctioning. See the following sections:
• TRACE 1300 Error Messages
• TRACE 1310 Error Messages

TRACE 1300 Error Messages

TRACE 1300 instrument malfunction, due to a component failure or to abnormal operating
condition is identified by the blinking of both Power and Ready lights located on the
TRACE 1300 Status Panel. See Figure 642.

Figure 642. TRACE 1300: Alarm Identification

A typical sound (beep) is heard. Power and temperatures are automatically cut off.
To reset an alarm, the GC must be powered off, and then powered on.

TRACE 1310 Error Messages

TRACE 1310 instrument malfunctioning, due to a component failure or to abnormal
operating condition, is identified by an error messages displayed before or during the runs.

IMPORTANT When an alarm is displayed in the message bar, try to solve the
problem, then go to the Diagnostics menu and press the Reset button to reboot
the system. If the problem persists, contact the Technical Support; see “Contacting
Technical Support” on page 589.

• FID Front/Back/Left/Right
– Unconnected
– Temperature over limit
– Opened PT100
– Shorted PT100
– Thermal safety: Not Heating or Auto Heating
– Reset detected
• ECD Front/Back/Left/Right
– Unconnected
– PLD error
– Temperature over limit

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 12 Troubleshooting
Error Messages

– Opened PT100
– Shorted PT100
– Thermal safety: Not Heating or Auto Heating
– Reset detected
• NPD Front/Back/Left/Right
– Unconnected
– Bead Current Over or Under range
– Temperature over limit
– Opened PT100
– Shorted PT100
– Thermal safety: Not Heating or Auto Heating
– Reset detected
– High Voltage shorted
• TCD Front/Back/Left/Right
– Unconnected
– Temperature over limit
– Opened PT100
– Shorted PT100
– Thermal safety: Not Heating or Auto Heating
– Reset detected
• FPD Front/Back/Left/Right
– Unconnected
– Temperature over limit
– Opened PT100
– Shorted PT100
– Thermal safety: Not Heating or Auto Heating
– Reset detected
– Opened Cell PT100
– Shorted Cell PT100
• PDD Front/Back/Left/Right
– Unconnected
– Temperature over limit

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12 Troubleshooting
Error Messages

– Opened PT100
– Shorted PT100
– Reset detected
• GDI Front/Back/Left/Right
– Unconnected
– Temperature over limit
– Opened PT100
– Shorted PT100
– Reset detected
• SSL-SSLBKF Front/back
– Unconnected
– Temperature over limit
– Opened PT100
– Shorted PT100
– Thermal safety: Not Heating or Auto Heating
– Reset detected
– Loss of Carrier
• PTV-PTVBKF Front/Back
– Unconnected
– Temperature over limit
– Opened Temperature Sensor
– Shorted Temperature Sensor
– Thermal safety: Not Heating or Auto Heating
– Reset detected
– Loss of Carrier
• AUX Temperature EXT-V Sub Ambient
– Unconnected
– X-Line B over limit
– X-Line B Opened PT100
– X-Line B Shorted PT100
– X-Line A over limit
– X-Line A Opened PT100

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Error Messages

– X-Line A Shorted PT100

– Thermal safety: Not Heating or Auto Heating
• Valve Oven
– Unconnected
– Heater B over limit
– Heater B Opened PT100
– Heater B Shorted PT100
– Heater A over limit
– Heater A Opened PT100
– Heater A Shorted PT100
– Thermal safety: Not Heating or Auto Heating Heater A or B
• AUX Carrier Primary
– Unconnected
– Channel 1 Loss of Carrier
– Channel 2 Loss of Carrier
– Channel 3 Loss of Carrier
• AUX Carrier Secondary
– Unconnected
– Channel 1 Loss of Carrier
– Channel 2 Loss of Carrier
– Channel 3 Loss of Carrier
• Oven
– Unconnected
– Temperature over limit
– Opened PT100
– Shorted PT100
– Thermal safety: Oven Auto Heating
– Hydrogen Sensor Alarm
– Hydrogen Sensor Fault
– Reset detected
– Thermal safety: Oven Not Heating
– Max Temperature reached

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Error Messages

• Main CPU
– Master Safety detected
– Main I2C Bus fault
– Detector I2C Bus fault
– EMI RAM Memory fault
– Main CPU Overheating
– Manifold Temperature Overheating
– 48Vac missing
– +24Vcc missing
– +15Vcc missing
– -15Vcc missing
– AC Mains out of range

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 12 Troubleshooting
Contacting Technical Support

Contacting Technical Support

If the information in this section does not help solve your problem, you should contact
Technical Support. Be sure to reference the model, serial number, and power supply of your
instrument when contacting them.
• Serial Number — You can find the serial number of the GC by opening the front door,
and reading the serial number label on the right lower corner. See Figure 643.

Figure 643. Identify Your Instrument: Serial Number

TRACE 1300/TRACE 1310 Serial

Number Label

• Power supply your GC is set — The 120 Vac or 230 Vac power supply is indicated on
the yellow label on the electronic module. See Figure 644.

Figure 644. Identify Your Instrument: Power Supply Data

Power Supply 120 Vac or

230 Vac Alert Label

For contacting your local Thermo Fisher Scientific office or affiliate GC-GC/MS Customer
Support, see the section “Contacting Us” on page xix.

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 G

Glossary
This section lists and defines terms used in this manual.
It also includes acronyms, metric prefixes, and symbols.
A B C D E F G H I J K L M N O P Q R S T V V W X Y Z

A <Ctrl> control key of the keyboard

A ampere D
ac alternating current d depth
ADC analog-to-digital converter DAC digital-to-analog converter
AOI Analog Output Interface dc direct current

B DS data system

b bit E
B byte (8 b) ECD Electron Capture Detector
baud rate data transmission speed in events per EMC electromagnetic compatibility
second
ESD electrostatic discharge
C
F
C Carbon
f femto
°C Celsius
ºF Fahrenheit
CDS Chromatography Data System
FID Flame Ionization Detector
CIP Carriage and Insurance Paid To
FOB Free on board
cm centimeter
FSE Field Service Engineer
CPU central processing unit (of a computer)
FPD Flame Photometric Detector

Thermo Scientific TRACE 1300 and TRACE 1310 Hardware Manual 591
Glossary: ft

ft foot K
FT-IR fourier transform infrared spectroscopy/fourier k kilo (103 or 1024)
transform infrared spectrometer
K Kelvin
G kg kilogram
g gram kPa kilopascal
GC gas chromatography- gas chromatograph
L
GDI Generic Detector Interface
l length
GND electrical ground
L liter
GSV Gas Sampling Valve
LAN Local Area Network
H lb pound
h height LED light-emitting diode
h hour
M
H Hydrogen
m meter (or milli [10-3])
harmonic distortion A high-frequency disturbance
that appears as distortion of the fundamental sine M mega (106)
wave
μ micro (10-6)
He Helium
MBq megabecquerel
HeS-S/SL Instant Connect Helium Saver Injector
Ci millicurie
HV high voltage
min minute
Hz hertz (cycles per second)
mL or ml milliliter
I mm millimeter

ID inside diameter MS mass spectrometry-mass spectrometer

IEC International Electrotechnical Commission m/z mass-to- charge ratio

Impulse See transient

N
in. inch
n nano (10-9)
I/O input/output
N Nitrogen

negative polarity The inverse of a detector signal

polarity.

592 TRACE 1300 and TRACE 1310 Hardware Manual Thermo Scientific
 Glossary: nm

nm nanometer SOP Standard Operating Procedures

NPD Nitrogen Phosphorous Detector SSL split/splitless injector

SSLBKF split/splitless injector with Backflush

O
source current The current needed to ignite a
OD outside diameter source, such as a detector lamp.
Ω ohm surge A sudden change in average RMS voltage level,
with typical duration between 50 μs and 2 s.
P
p pico (10-12)
T

Pa pascal TCD Thermal Conductivity Detector

PCB printed circuit board transient A brief voltage surge of up to several

thousand volts, with a duration of less than 50 μs.
PDD Pulsed Discharge Detector
V
PN part number
V volt
psi pounds per square inch
Vac volts, alternating current
PTV Programmable Temperature Vaporizing Injector
Vdc volts, direct current
PTVBKF Programmable Temperature Vaporizing
Injector with backflush VGA Video Graphics Array

R W
RAM random access memory w width
<Return> <Return> key on the keyboard W Watt
RF radio frequency
When a unit of measure has a quotient (e.g. Celsius
ROM read-only memory degrees per minute or grams per liter) this can be written
as negative exponent instead of the denominator:
RS-232 industry standard for serial communication
For example:
°C min-1 instead of °C/min
S
g L-1 instead of g/L
s second

sag See surge

slow average A gradual long-term change in average

RMS voltage level, with typical duration greater than
2 s.

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