Bangladesh University of Engineering and Technology

Department of Biomedical Engineering

BME 204: Human Physiology Sessional

Lab 06: Spirometric Measurement of Lung Function Test by Determination of

FVC, FEV and MVV with Interpretation and Analysis of Results.

Brief Introduction to Pulmonary Functions

The respiratory or pulmonary system performs the important functions of supplying oxygen (O2)
during inhalation, removing carbon dioxide (CO2) during exhalation, and adjusting the acid-base
balance (pH) of the body by removing acid-forming CO2. Because oxygen is necessary for cellular
metabolism, the amount of air that the pulmonary system provides is important in setting the
upper limits on work capacities or metabolism. Therefore, the measurement of lung volumes and
the rate of air movement (airflow) are important tools in assessing the health and capacities of a
person. In this experiment, you will measure: -

 Forced Vital Capacity (FVC), which is the maximal amount of air that a person can
forcibly exhale after a maximal inhalation.
 Forced Expiratory Volume (FEV), which is the percentage of FVC that a person forcibly
expels in intervals of 1, 2, and 3 seconds (FEV1, FEV2, FEV3).
 Maximal Voluntary Ventilation (MVV), which is a pulmonary function test that combines
volume and flow rates to assess overall pulmonary ventilation.

These measurements indicate the upper limit of work that the person can do based on the
capabilities of his or her respiratory system. When a person takes in maximal inhalation and then
follows this with maximal exhalation the volume of expired air is that person’s Single Stage Vital
Capacity (SSVC). The time required to achieve maximal exhalation is not a factor in determining
SSVC. Because the lungs reside in the thoracic cavity, vital capacity is ultimately restricted by
the size of a person’s thoracic cavity. Therefore, size-related variables (e.g., age, gender, weight)
affect the capacities of the respiratory system.

Equations for Predicted Vital Capacity

Male V.C. = 0.052H - 0.022A – 3.60

Female V.C. = 0.041H - 0.018A – 2.69

where V.C=Vital Capacity is in liters, H=Height in centimeters and A=Age in years.

Using this equation, you can estimate the vital capacity of a 19 year old female who is 167
centimeters tall (about 5’6”) as 3.815 liters: 0.041 x (167) – 0.018 x (19) – 2.69 = 3.815 liters.

For adults, the average pulmonary capacities decrease with age. Women tend to have smaller
volumes than men of the same age and weight. As weight increases, volumes increase, with the
exception that overweight people tend to have decreased volumes. Even within one person,
respiratory supply and demand differs with activity levels and health. Accordingly, the rate and
depth of ventilation (the volume of gas you breathe in and out per minute) are not static but
rather must constantly adjust to the changing needs of the body. As you increase your activity

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levels from rest, the volume and rate of air flowing in and out of your lungs also changes. The
changes in volume and how fast those changes in volume (airflow) are effected can be used to
assess the health of a person’s respiratory system.

Pulmonary volumes, pulmonary capacities, and pulmonary airflow rates are often measured in
diagnosing and assessing the health of the respiratory system.

In general, chronic pulmonary diseases may be classified into two physiologic categories:

1. Chronic obstructive pulmonary disease (COPD)

2. Chronic restrictive pulmonary disease.

Chronic obstructive pulmonary disease (COPD)

In obstructive pulmonary disease, such as asthma or emphysema, airflow into and out of the lungs
is reduced. In asthma, inflammation of the lining of the airways and heavy mucus secretion reduce
airway diameters and increase airway resistance. This results in a wheezing sound (characteristic
of asthmatic breathing) and a reduction in the volume of air flowing into and out of the lungs per
minute. Diagnosis of obstructive pulmonary diseases usually requires measurements of pulmonary
flow rates and ERV.

Chronic restrictive pulmonary disease (CRPD)

In restrictive pulmonary disease, the person’s ability to inflate and deflate the lungs is reduced,
and as a result, some lung volumes and capacities are below normal. For example, in pulmonary
fibrosis (such as occurs in coal miner’s disease), vital capacity, (the maximal air volume moved out
of the lungs) is reduced. Vital capacity is reduced both because of reductions in inspiratory and
expiratory reserve volumes, volumes beyond normal resting tidal volume. This also occurs in

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silicosis or other chronic diseases of the lung in which the lungs become less distensible. Restrictive
pulmonary diseases are diagnosed, in part, by determining lung volumes and capacities. It is not
uncommon for a person to have restrictive and obstructive pulmonary diseases simultaneously,
even though each disease may have a different origin and may have begun at a different time. For
example, a person may suffer from emphysema and fibrosis of the lung at the same time.

In this experiment, you will perform two tests to measure pulmonary flow rates:

1. Forced Expiratory Volume (FEV)

2. Maximal Voluntary Ventilation (MVV)

Test #1: Forced Expiratory Volume (FEV)

Forced Expiratory Volume (also referred to as forced vital capacity or timed vital capacity) is a
test in which a limit is placed on the length of time a Subject has to expel vital capacity air.
FEV1, FEV2, FEV3 are defined as the percentage of vital capacity that can be forcibly expelled
after a maximal inhalation in the period of one second, two seconds, and three seconds,
respectively.

The normal adult is able, with maximal effort, to expire about 66-83% of his/her vital capacity in
one second (FEV1.0), 75-94% of their vital capacity in the second second (FEV2.0), and 78-97% of
their vital capacity by the end of the third second (FEV3.0).

A person with asthma may have a normal or near-normal vital capacity as measured in a Single
Stage Vital Capacity test, which allows as long as necessary to maximally inhale and exhale.
However, when an asthmatic exhales vital capacity with maximal effort, FEV measurements are
all reduced because heavy mucus secretion and smooth muscle action reduces airway diameter and
it takes longer to completely exhale vital capacity against increased airway resistance.

Test #2 Maximal Voluntary Ventilation (MVV)

The Maximal Voluntary Ventilation (also known as maximal breathing capacity) measures peak
performance of the lungs and respiratory muscles. MVV is calculated as the volume of air moved
through the pulmonary system in one minute while breathing as quickly and deeply as possible
(hyperventilation). In performing this test, the Subject inspires and expires as deeply and as
rapidly as possible (> 1 breath/sec) while the tidal volume and the respiratory rate are measured.
Because the maximal breathing rate is difficult to maintain, the Subject hyperventilates for a

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maximum of 15 seconds. Then, to calculate MVV, the average volume per respiratory cycle (liters)
is multiplied by the number of cycles per minute (liters/min). MVV can also be extrapolated from
the total volume of air moved in a 12-sec period (total volume in 12- sec X 5 = MVV).

Normal values vary with sex, age and body size. MVV is a measure of how much your pulmonary
system limits your capacity to work or exercise.

You can rarely exceed your MVV, even for brief periods of time. Therefore, MVV ultimately limits
how much oxygen is available for exercising muscles. In general, a maximum of 50% of your MVV
can be used for exercise beyond 10 minutes. Most people have trouble breathing when only using
the available 30-40% MVV. MVV tends to be reduced in both restrictive and obstructive
pulmonary diseases.

Experimental Objectives

 To observe experimentally, record, and/or calculate forced expiratory volume (FEV) and
maximal voluntary ventilation (MVV).
 To compare observed values of FEV with predicted normals.
 Compare MVV values with others in your class.

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On concluding the experiment, it is expected that the students will be able to answer the following
questions in light of the experiment.
D. Define Forced Expiratory Volume (FEV).
E. How do Subject’s FEV values compare to the average?
FEV1.0 less than same as greater than
FEV2.0 less than same as greater than
FEV3.0 less than same as greater than
F. Is it possible for a Subject to have a vital capacity (single stage) within normal range but a value
for FEV1.0 below normal range? Explain your answer.
G. Define Maximal Voluntary Ventilation (MVV.)
H. How does Subject’s MVV compare to others in the class? less than same as greater
than
I. Maximal voluntary ventilation decreases with age. Why?
J. Asthmatics tend to have smaller airways narrowed by smooth muscle constriction, thickening of the
walls, and mucous secretion. How would this affect vital capacity, FEV 1.0, and MVV?
K. Bronchodilator drugs open up airways and clear mucous. How would this affect the FEV and MVV
measurements?
L. Would a smaller person tend to have less or more vital capacity than a larger person?

M. How would an asthmatic person’s measurement of FEV1.0 and MVV compare to an athlete? Explain
your answer.

General Requirements

1. All students must Conduct the experiment as dictated by the lab manual and
document it according to the requirements of Assessment Rubrics and get it approved
by the Lab Instructor after conducting any experiment.
2. You should be prepared to demonstrate your experimental setup and answer questions
in all aspects related to your experiment.
3. You should work in groups of 7 students each. One report addressing all parts of
Assessment Rubrics should be submitted on behalf of the whole group.
4. You may use any resources you find useful to your experiment as long as you
acknowledge such use in your report in accordance to ethical guidelines.

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 Assessment Rubrics
Exemplary Satisfactory Developing Unsatisfactory
KPI’s 3 2 1 0
Conducts the experiment Experimental Set-up is Experimental Set-up is Experimental Set-up is Experimental Set-up is
always neat and accurate. mostly neat and accurate. workable with minor help. mostly untidy and
Always records complete Mostly records complete Recordsincomplete data inaccurate. Rarely records
data and identify possible data and identify possible e.g., sampling (number of and collects data in the
sources of error. sources of error. data points) is just format prescribed in the
Measurements are always Measurements are mostly sufficient, understands lab manual.
accurate with symbols, accurate with symbols, possible sources of error Measurements are
units and significant units and significant with minor help. inaccurate and often
digits. Collects data digits. Collectsdata Measurements are less without symbols, units
always in the format mostly in the format accurate with some errors and significant digits.
prescribed in the lab prescribed in the lab in symbols, units and Does not demonstrate
manual. Always manual. Mostly significant digits. Collects reproducibility as well as
demonstrates demonstrates data that are sometimes required knowledge of lab
reproducibility and good reproducibility and good difficult to handle and procedures.
knowledge of lab knowledge of lab understand. Lacks
procedures. procedures. reproducibility in results
and demonstrates some
knowledge of lab
procedures.

Analyzes and interprets Comprehensively Sufficiently understands Fairly understands the Poorly understands the
data understands the data in the data in terms of data in terms of variables data in terms of variables
terms of variables variables (dependent/independent), (dependent/independent),
(dependent/ (dependent/independent), assumptions, deviations assumptions, deviations
independent), assumptions, deviations and experimental and experimental
assumptions, deviations and experimental uncertainties etc. uncertainties. Fails to
and experimental uncertainties etc. Organizes the data in Organize the data in
uncertainties etc. Organizes the data in figures and tables using figures and tables using
Organizes the data in figures and tables using modern software tools modern software tools.
figures and tables using modern software tools fairly for analysis. Fails to Discuss/compare
modern software tools sufficiently for analysis. Discusses/compares his/her results in the light
extensively for analysis. Discusses/compares his/her results in the light of obtained
Discusses/compares his/her results in the light of obtained results/ results/theoretical models
his/her results in the light of obtained theoretical models of of similar studies from
of obtained results/theoretical models similar studies from other other sources. Fails to
results/theoretical models of similar studies from sources fairly. Concludes conclude rationally based
of similar studies from other sources sufficiently. based on his/her on experimentation and
other sources extensively. Concludes rationally experimentation and acceptable reasoning.
Concludes rationally based on experimentation acceptable reasoning.
based on experimentation and fair reasoning.
and clear reasoning.

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Experimental Protocol

MATERIALS
 BIOPAC Airflow Transducer SS11LB.
 BIOPAC Bacteriological Filter (AFT1): one per subject
 BIOPAC Disposable Mouthpiece (AFT2)
 BIOPAC Noseclip (AFT3)
 Biopac Student Lab System: BSL 4 software, MP36 hardware
 Computer System (Windows or Mac)

EXPERIMENTAL METHODS
A. SETUP
FAST TRACK Setup Detailed Explanation of Setup Steps
1. Turn your computer ON.
2. Turn OFF MP36/35 unit.
3. Plug the Airflow Transducer (SS11LA) into
Channel 1.
4. Turn ON the MP36/35 unit.

Setup continues… Fig. 1 MP35 (top) equipment connections

5. Start the Biopac Student Lab program. Start Biopac Student Lab by double-clicking the Desktop
6. Choose “L13 – Pulmonary Function II” shortcut.
and click OK.
7. Type in a unique filename and click OK.

No two people can have the same filename, so use a unique

identifier, such as Subject’s nickname or student ID#.
A folder will be created using the filename. This same filename
can be used in other lessons to place the Subject’s data in a
common folder.

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B. CALIBRATION
Calibration establishes the hardware’s internal parameters (such as gain, offset, and scaling) and is critical
for optimal performance. Calibration will vary based on the Preference set by your lab instructor.
FAST TRACK Calibration Detailed Explanation of Calibration Steps

1. Hold the Airflow Transducer upright Calibration Stage 1 precisely

and still, making sure no air is flowing zeroes the baseline. Any baseline
through it (Fig. 2). shift during this calibration can
cause errors in the subsequent
recordings. Baseline shift can
occur from:
a) Airflow through the
transducer from movement,
an HVAC duct or even from
breathing close to the unit.
Stage 1 – Always required

b) Changes in transducer Fig. 2

orientation. The transducer
should be held still and in
the same orientation that
will be used during the
recording.
2. Click Calibrate. Calibration lasts from 4 to 8 seconds.
 Wait for Calibration to stop
3. Check Calibration data:
 Verify data is flat and centered. If
necessary, click Redo Calibration.
 To proceed, click Continue.

Fig. 3 Example Calibration Stage 1 data

C. DATA RECORDING
FAST TRACK Recording Detailed Explanation of Recording Steps
1. Prepare for the recording. In this recording, two conditions will be performed to measure
 Remove Calibration Syringe/filter pulmonary flow rates:
assembly (if used). Forced Expiratory Volume (FEV)
Maximal Voluntary Ventilation (MVV)
Each test will be saved as a separate data file.
Hints for obtaining optimal data:
 Review onscreen “Tasks” to prepare for the recording steps in
advance.
 Subject should wear loose clothing so clothing does not
inhibit chest expansion.
 Subject must try to expand the thoracic cavity to its largest
volume during maximal inspiratory efforts.
 Air leaks will result in inaccurate data. Make sure all
connections are tight, noseclip is attached and that Subject’s
mouth is sealed around the mouthpiece.
 Keep the Airflow Transducer vertical and in a constant

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 position. (Fig. 5).

2. Insert the filter into the “Inlet” side of IMPORTANT: Each Subject must use a personal filter,
the transducer, and then attach the mouthpiece and noseclip. The first time they are used, the
mouthpiece (Fig. 4). Subject should personally remove them from the plastic
 If your lab does not use disposable packaging.
filters, attach a sterilized mouthpiece
(AFT8) directly to the “Inlet” side of
the transducer (Fig. 4).

Fig. 4 SS11LB with reusable filter/mouthpiece combination

Setup continues...
3. Prepare the Subject: Verify there are no air leaks; mouthpiece and filter are firmly
 Subject must be seated, relaxed and attached, the noseclip is snug and the Subject’s mouth is tightly
still, facing away from the monitor. sealed around mouthpiece.
 Place noseclip on Subject’s nose.
 Subject holds Airflow Transducer
vertically, breathing through
mouthpiece.
 Before recording, Subject acclimates
by breathing normally for 20 seconds.
 Review recording steps.

Fig. 5 Keep Airflow Transducer upright

at all times

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 Part 1 — FEV

4. Click Record FEV. 1 cycle = inspiration + expiration

5. Subject performs the following procedure: After maximum inspiration, hold breath for an instant so that
 Breathe normally for three cycles. when analyzing the data the beginning of the exhale can be
clearly seen.
 Inhale as deeply as possible
(maximum inspiration). For the maximum expiration, it is important to expel all air,
which should take more than 3 seconds.
 Hold breath for just an instant.
 Forcefully and maximally exhale
(maximum expiration).
 Resume normal breathing for three
more cycles.
6. Click Stop. If a recording is started on an inhale, try to stop recording on an
exhale, or vice versa.
Upon Stop, the Biopac Student Lab software will automatically
convert the air flow data to volume data as shown in Fig. 6.

7. Verify recording resembles the example The maximal inhale and maximal exhale should be clearly visible
data. in the data and there should be three normal breathing cycles
 If similar, click Continue and proceed both before and after.
to the next recording.

Recording continues…

Fig. 6 Example FEV Data

 If necessary, click Redo. If recording does not resemble the Example Data
 If the data is noisy or flatline, check all connections to the
MP unit.
 If there are not three normal breathing cycles on either side
of the maximal inhale/exhale, Redo the recording.
 If it is difficult to determine the beginning of maximal
expiration, the Subject may not have held breath for an
instant after maximal inhalation; consider redoing the
recording.
 If the maximal inhale/exhale data is not much greater in
amplitude than that during normal breathing; verify there are
no air leaks; mouthpiece and filter are firmly attached, the
noseclip is snug and the Subject’s mouth is firmly sealed
around the mouthpiece.
Click Redo and repeat Steps 4 – 6 if necessary. Note that once
Redo is clicked the data will be erased.
8. Zoom in using the zoom tool, on the area The selected area should include some data both before and after
of maximal exhale. maximal exhale.
9. Use the I-beam cursor to select the area The left mouse button is held down while selecting with the I-
of from beginning of maximal expiration beam cursor.
to the end of maximal expiration. At
least three seconds must be selected (Fig. The first measurement box will display Delta T so you can make

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 7). sure the selected area is longer than 3 seconds.
If Delta T is less than 3 seconds, the Subject may not have
expelled all air during maximum expiration. Click Redo and
repeat Steps 4 – 6 if necessary.

Fig. 7 Selected area for maximal exhale

10. Click Calculate FEV. The program will cut out the selected area, invert it, zero the
offset, and paste it into a new channel (Fig. 8). The original
volume data will be deleted.
11. Verify the FEV plot resembles the If the data was selected properly in Step 8, the first data sample
example data. should be the minimum (0 Liters) and the data should continue
 If similar, click Continue and proceed to increase for at least 3 seconds.
to the MVV recording.
 If necessary, click Redo to reselect
area of maximal exhale and recalculate
FEV.
 If you will not be recording MVV,
click Doneand proceed to the Data
Analysis section.

Fig. 8 Example FEV Plot

Upon Continue, the FEV data will be automatically saved for
Recording continues… later analysis.

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 Part 2 — MVV
12. Prepare the Subject.
 Subject must be seated, relaxed and
still, facing away from monitor.
 Place noseclip on Subject’s nose.
 Subject holds airflow transducer
vertically, breathing through
mouthpiece.
 Subject breathes normally for 20
seconds prior to starting recording.
 Review recording steps.
13. Click Record MVV
14. Subject performs the following procedure:
 Breathe normally for five cycles. 1 cycle = inspiration + expiration
 Breathe quickly and deeplyfor 12 – 15 WARNING: This procedure can make Subject dizzy and light
seconds. headed. Subject should be sitting down and Director should watch
Subject. Stop the procedure if Subject feels sick or excessively dizzy.
 Breathe normally for five
additionalcycles.
15. Click Stop. Upon Stop, the Biopac Student Lab software will automatically
16. Verify that recording resembles the convert the airflow data to volume data as shown in Fig. 9.
example data. The rapid, deep breathing data should be clearly visible in the
 If similar, proceed to Step 15. data.

Fig. 9 Example MVV data

Note:
The software zeros the baseline
after each cycle, which can result
in data resembling the example to
the right. It is not necessary to
redo the recording, as data
analysis will not be affected.

Recording continues… Fig. 10 Example of baseline

reset

 If necessary, clickRedo. If recording does not resemble the Example Data

 If the data is noisy or flatline, check all connections to the MP

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 unit.
 If the rapid, deep breathing data is not much greater in
amplitude than that during normal breathing; verify there are
no air leaks; mouthpiece and filter are firmly attached, the
noseclip is snug and the Subject’s mouth is firmly sealed
around the mouthpiece.
Click Redo and repeat Steps 13 – 15 if necessary. Note that once
Redo is clicked the data will be erased.
17. Click Done. This lesson creates two data files; one for FEV data and one for
18. Choose an option and click OK. MVV data, as indicated by the file name extension.
When Done is clicked, a dialog with options will be generated.
Make a selection and click OK.
If FEV and MVV recordings were both performed, choosing the
“Analyze current data file” option will open the MVV file, but the
FEV data file should be opened first, as this file is referenced in
Part 1 of the Data Analysis section that follows.
To open the FEV file first, choose “Analyze another data file” from
the list of options and navigate to the correct “FEV – L13” file in
the Subject’s folder.

If choosing the “Record from another Subject” option:

 Repeat Calibration Steps 1 – 3, and then proceed to

Recording.

END OF RECORDING

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 II. DATA ANALYSIS
FAST TRACK Data Analysis Detailed Explanation of Data Analysis Steps
1. Enter the Review Saved Data mode. If entering Review Saved Data mode from the Startup dialog or
Lessons menu, be sure to choose the file with “FEV – L13” file name
extension.
 Note channel number (CH)
designations:
Channel Displays
CH 2 Volume

 Note measurement box settings:

Channel Measurement
CH 2 Delta T
CH 2 P-P

Fig. 11 Example FEV data

The measurement boxes are above the marker region in the data
window. Each measurement has three sections: channel number,
measurement type, and result. The first two sections are pull-down
menus that are activated when you click them. The following is a
brief description of these specific measurements.
Brief definition of measurements:
Delta T: Displays the amount of time in the selected area (the
difference in time between the endpoints of the selected area).
P -P(Peak-to-Peak): Subtracts the minimum value from the
maximum value found in the selected area.
The “selected area” is the area selected by the I-Beam tool (including
endpoints).
Useful tools for changing view:
Display menu: Autoscale Horizontal, Autoscale Waveforms, Zoom
Back, Zoom Forward
Scroll Bars:Time (Horizontal); Amplitude (Vertical)
Cursor Tools: Zoom Tool
Buttons: Show Grid, Hide Grid, -, +
2. Use the I-beam cursor to select the The P-Pmeasurement for the selected area represents the Vital
area from time zero to the end of the Capacity (VC).
recording. Record the Vital Capacity
(VC). Note: In the example, the Grids have been enabled to assist in data
selection.
A

Fig. 12 All data selected

Data Analysis continues…

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3. Use the I-beam cursor to select the first The selected area should be from
one-second interval (Fig. 13).Record Time 0 to the one-second reading,
the volume expired and calculate as displayed in the Delta T
FEV1.0. measurement. The grid can be used
B as a reference. The volume expired
is indicated by the P-P
Fig. 13 FEV1.0
measurement.
4. Use the I-beam cursor to select the first
two-second interval (Fig. 14).Record
the volume expired and calculate
FEV2.0.
B

Fig. 14 FEV2.0

5. Use the I-beam cursor to select the first

three-second interval (Fig. 15). Record
the volume expired and calculate
FEV3.0.

B

Fig. 15 FEV3.0

6. Select File>Save Changes.

7. Pull down the Lessons menu, select Choose the data file that was saved with “MVV – L13” extension.
Review SavedData, and choose the
correct MVV – L13 file.
8. Use the zoom tool to set up your
display window for optimal viewing of
the deep, fast breathing portion of the
recording (Fig. 16).

Fig. 16 Zoom in on rapid/deep breathing data

9. Use the I-beam cursor to select a Use the Delta T measurement to determine the time interval. In the
twelve-second area that is convenient example below, 13 cycles are in the 12 second interval.
to count the number of cycles in the
interval (Fig. 17).
C

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 Data Analysis continues…

Fig. 17 Example of 12 second data selection

10. Place an event marker at the end of It’s helpful to clearly

the 12 second selected area (Fig. 18). mark the end of the
individual cycle
measurement area
byplacing an event
marker at the end of the
selected 12 second
interval. To place an
event marker, right-click Fig. 18 Event Marker insertion
in the marker region just
above the data display
and select “Insert New
Event.”If the event
marker is not placed
correctly, it can be moved
by holding down the Alt
key and dragging with the
mouse.
You may also enter event
text in the field above the
marker.
11. Use the I-beam cursor to select each The Volume is measured by the P -P (Peak-to-Peak) measurement.
complete individual cycle in the 12- Fig.19 shows the first cycle of the 12-second interval defined in Fig.
second interval defined in Step 9.
Record the volume of each cycle. 18 selected:

C
12. Calculate the average volume per cycle
(AVPC) and then the Maximal
Voluntary Ventilation (MVV).

C

Fig. 19 Example of first cycle selection

13. Quit the program.

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Experiment#06 Datasheet
 Pulmonary Flow Rates
 Forced Expiratory Volume (FEV)
 Maximal Voluntary Ventilation (MVV)
DATA REPORT
Students’ Names (with IDs) :
_________________________________________________________________
_________________________________________________________________
_________________________________________________________________
_________________________________________________________________
Group No.:
Date:

Subject Profile
Name: Height:

Age: Gender: Male /Female Weight:

I. Data and Calculations

A. Vital Capacity (VC)

= ________________

B. Forced Expiratory Volumes: FEV1.0, FEV2.0, FEV3.0

Table 1
Time Forced Expiratory Vital Capacity (FEV/VC) =FEVx NormalAdultR
Interval Volume (VC) ange
(sec) FEV/VC x 100 = %
from A
calculate calculate

0-1 % FEV1.0 66% - 83%

0-2 % FEV2.0 75% - 94%
0-3 % FEV3.0 78% - 97%

C. MVV Measurements (Note, all volume measurements are in liters)

1) Number of cycles in 12-second interval: ______
2) Calculate the number of respiratory cycles per minute (RR):
RR = Cycles/min = Number of cycles in 12-second interval x 5
Number of cycles in 12-second interval (from above): ______ x 5 = ______cycles/min
3) Measure each cycle

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 Complete Table 2 with a measurement for each individual cycle. If Subject had only 5 complete
cycles/12-sec period, then only fill in the volumes for 5 cycles. If there is an incomplete cycle, do not
record it. (The Table may have more cycles than you need.)

Table 2

Volume Volume
Cycle Cycle Number
Measurement Measurement
Number

Cycle 1 Cycle 9

Cycle 2 Cycle 10

Cycle 3 Cycle 11

Cycle 4 Cycle 12

Cycle 5 Cycle 13

Cycle 6 Cycle 14

Cycle 7 Cycle 15

Cycle 8 Cycle 16

4) Calculate the average volume per cycle (AVPC):

Add the volumes of all counted cycles from Table 13.3.

Sum = ________________ liters

Divide the above sum by the number of counted cycles. The answer is the average volume per cycle
(AVPC)

AVPC = _______ / ____________________ = ____________________liters

Sum # of counted cycles

5) Calculate the MVVest

Multiply the AVPC by the number of respiratory cycles per minute (RR) as calculated earlier.

MVV = AVPC x RR = _______ x _________ = __________________liters/min

AVPC RR

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