PROGRAM BOOK

International Symposium on Innovative Approaches to

Livestock Industry (ISIALI 2025)

IPB International Convention Center, Bogor-Indonesia

January 21, 2024

Organized by
School of Veterinary Medicine and Biomedical Sciences IPB University
Faculty of Animal Science IPB University

Supported by
Korea International Cooperation Agency (KOICA)

International Symposium on Innovative Approaches to Livestock Industry 2025 | 1

 Table of Contents
Table of Contents.........................................................................................................3
Guidelines of Presentation...........................................................................................9
SEMINAR PROGRAM FIRST DAY........................................................................11
SEMINAR PROGRAM SECOND DAY..................................................................12
CV OF SPEAKERS...................................................................................................13
PLENARY SESSION (FIRST DAY)........................................................................27
PLENARY SESSION (SECOND DAY)...................................................................33
ORAL PRESENTATION (FIRST DAY)...................................................................37
ORAL PRESENTATION (SECOND DAY)..............................................................75
POSTER PRESENTATION.....................................................................................119
List of ICARE Committee.......................................................................................141

2 | International Symposium on Innovative Approaches to Livestock Industry 2025

International Symposium on Innovative Approaches to Livestock Industry 2025 | 3
 INTERNATIONAL SYMPOSIUM ON
INNOVATIVE APPROACHES TO LIVESTOCK
INDUSTRY 2025
PROGRAM SCHEDULE

Tuesday, January 21, 2025

Time Event
08.00-08.30 Registration
08.30-08.55 Opening Remark
Prof. Dr. Arif Satria, SP, M.Si
Rector of IPB University
08.55-09.20 Opening Remark
Dr. Ir. Dadan Hindayana
National Nutrition Agency, Indonesia
09.20-09.50 Opening Remark
Dr. drh. Agung Suganda, M.Si
DG of Livestock and Animal Health, Ministry of Agric
09.50-10.15 Group Photo and Coffee break
Plenary Session 1
Moderator: Prof. Byung-Chul Park
10.15-10.35 Invited speaker
Title: (TBA)
Prof. Kyoung Hoon Kim
GSIAT, Seoul National University
10.35-10.55 Invited speaker
Title: Feed Additive
Prof. Ahmad Sofyan, S.Pt, M.Sc., Ph.D
National Research and Innovation Agency (BRIN)
10.55-11.25 Invited speaker
Title: (TBA)
Prof. Dr. Ir. Drajad Martianto, M.Si
Inter-Univ. Center for Excellence for Food Security
11.25-13.00 Lunch Break
Plenary Session 2
Moderator: Prof. Dr. Ir. Komang G. Wiryawan
13.00-13.30 Invited speaker
Topic: Dairy cattle
Prof. Sang Kee Kang
GSIAT, Seoul National University
13.30-14.00 Invited speaker

4 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Title: Innovative Feeding Strategy to Enhance Milk Quality
and Rumen Efficiency: A Pathway to Sustainable Food
Security in Indonesia
Prof. Dr. Despal, S.Pt. MSc.Agr
Faculty of Animal Science, IPB University
14.00-14.30 Invited speaker
Title: Palmofeed: A Functional Poultry Feed Based on
Hydrolyzed Palm Kernel Meal
Prof. Dr. Ir. Nahrowi, M.Sc
Faculty of Animal Science, IPB University
14.30-15.00 Invited speaker
Title: Development of Wagyu Cattle in Indonesia by
Embryo Transfer Technology
Prof. drh. Arief Boediono, PhD PAVet
School of Veterinary Medicine and Biomedical, IPB
University
15.00-15.30 Coffee Break
Plenary Session 3
Moderator: Prof. Tae Sub Park
15.30-16.00 Invited speaker
Title: Meat processing and safety systems in Korea
Prof. Gap-don Kim
GSIAT, Seoul National University
16.00-16.30 Invited speaker
Title: The Current Vaccine Technology for Livestock in
Indonesia
Dr. drh. Okti Nadia Putri, M.Si, M.Sc
School of Veterinary Medicine and Biomedical, IPB
University
16.30-17.00 Invited speaker
Topic: Insect for Food and Feed
Prof. Dr. Ir. Dewi Apri Astuti, MS
Faculty of Animal Science, IPB University
17.00-17.30 Invited speaker
Title: Genetic Improvement of Economically Important
Traits in Indonesian Local Livestock
Prof. Dr. Asep Gunawan, S.Pt, MSc
Faculty of Animal Science, IPB University
17.30 Wrap-up Session

International Symposium on Innovative Approaches to Livestock Industry 2025 | 5

 CV OF SPEAKERS

6 | International Symposium on Innovative Approaches to Livestock Industry 2025

Prof. Kyoung Hoon Kim

Ermias Kebreab is a Professor of Animal Science and Associate

Dean at the University of California, Davis, where he holds the
Sesnon Endowed Chair in Sustainable Agriculture. He conducts
research on climate smart animal production and improving food
security. Dr. Kebreab is a globally recognized expert, having
contributed to the 2019 Intergovernmental Panel on Climate Change
(IPCC) update on enteric methane emissions and co-chaired
committees on feed additive and methane for the United Nations Food and
Agriculture Organization. He has authored over 250 peer-reviewed articles and has
received numerous awards, including the Excellence in Ruminant Nutrition and
International Agriculture awards from the American Society of Animal Science, as
well as the 2022 and 2024 Chancellor’s Innovator of the Year and Global
Engagement awards. Dr. Kebreab has also served on two committees of The
National Academies of Sciences, Engineering, and Medicine, focusing on methane
and the nutrition of dairy cattle. As a sought-after speaker, he delivered a TED talk
highlighted as a "must-watch climate talk of 2022" by ted.com. His research was
ranked in the top 10 of all research conducted within the entire University of
California system in 2021, and he was listed among the top 30 global leaders
working toward climate solutions in 2023 by Business Insider. Dr. Kebreab
completed his undergraduate studies at the University of Asmara, Eritrea, and
earned his M.S. and Ph.D. from the University of Reading, U.K.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 7

Prof. Ahmad Sofyan, S.Pt, M.Sc., Ph.D
Senior Lecturer in Microbiology, School of Veterinary Medicine,
Murdoch University
Member, Centre for Animal Production and Health, Food Futures
Institute
Member, Centre for Biosecurity and One Health, Harry Butler
Institute

 Summary: A degree-qualified and highly experienced academic and researcher

who has held positions across universities and laboratories in different countries.
Strengths include well-developed communication skills across a broad range of
interpersonal and institutional contexts, administrative excellence, excellent
collaborations with industries and universities nationally and internationally, and
grants success. Skilled in managing research projects and experienced in leading
research groups, student supervision and mentoring. Experienced of actively
working in Germany, Bangladesh and Australia in teaching, diagnostic and
research related to Veterinary Virology, Animal health and production,
Immunology, Biosecurity, and Molecular Genetics and Genomics.
 Previous experiences:
 Associate Professor, Faculty of Veterinary Science, Bangladesh Agricultural
University, Bangladesh
 Group Leader, Molecular biology of pig group, Institute of Animal Science,
University of Bonn, Germany
 Lecturer, School of Veterinary Science, University of Queensland, Australia
 Virologist, Biosecurity Sciences Laboratory, Department of Agriculture and
Fisheries, Queensland Government, Australia
 Educational qualification:
 Postdoctoral Researcher: School of Veterinary Science, UQ, Australia, and
Institute of Animal Science, University of Bonn, Germany
 PhD: Faculty of Agriculture, University of Bonn, Germany
 Master of Science (MS), Doctor of Veterinary Medicine (DVM): Bangladesh
Agricultural University
 Research grants: attracted research grants worth more than three-million AUD.
Some notable projects are:
 Exploring nature's antimicrobial arsenals to combat resistant bacteria in pigs
(Australasian Pork Research Institute: $925,000).
 National Surveillance of Antimicrobial Bacteria in Retail Food (Food Standards
Australia New Zealand, $701,628).
 Evaluation of single cell protein products in piglet diets (Woodside Energy Ltd:
$75,000 and $115,000).
 Growth, immune and antioxidant responses of heat stress-exposed broilers fed
diets supplemented and with different methionine sources (Evonik Australia
Ltd: $373,000 and $369,000).
 Research collaborations: excellent national and international collaborations.
Key collaborators are:

8 | International Symposium on Innovative Approaches to Livestock Industry 2025

  Prof Karl Schellander, University of Bonn, Germany
 Prof Sam Abraham, Murdoch University, Australia
 Prof Asep Gunawan, IPB/Bogor Agricultural University, Indonesia
 Prof Mehmet Ulas Cinar, Erciyes University, Turkey
 Prof Autchara Kayan, Kasetsart University, Thailand
 Prof M Siddiqur Rahman, Bangladesh Agricultural University
 Publication track record:
 Number of peer reviewed article: 85, and Conference abstract: 61
 Total citations: 2090 (as of 30 Sep 2024), and H-index: 26.
 Teaching experiences:
 Conducted 18 courses in Veterinary and Animal Science at the undergraduate
and postgraduate levels in the past 18 years of academic career.
 Associate Fellow of the Higher Education Academy (AFHEA).
 Supervisions:
 Worked with a total of 16 HDR students. Most of them are working in
academia.
 Currently supervising 5 PhD students, of which 3 as principal supervisor.
 Professional services:
 Associate Editor: BMC Res Notes; Frontiers in Vet Sci; PLoS One.
 Editorial Board member: Bang J Vet Med; Res in Agri Livest Fish.
 Reviewing articles regularly for reputed journals
 Awards:
 University Gold Medal
 Global Research Impact Award
 Public profiles:
 http://profiles.murdoch.edu.au/myprofile/jasim-uddin/
 https://scholar.google.com.au/citations?user=o_sKX5QAAAAJ&hl=en
 https://www.researchgate.net/profile/Muhammad_Jasim_Uddin
 https://orcid.org/0000-0002-6732-5534
 Contact details:
School of Veterinary Medicine, College of Science, Health, Engineering &
Education
Tel: +61 8 9360 7418, Email [email protected]
Room 3.050, Building 250, 90 South Street, Murdoch, WA 6150, Australia

International Symposium on Innovative Approaches to Livestock Industry 2025 | 9

Prof. Dr. Ir. Drajad Martianto, M.Si
Maja Slingerland, currently associate professor at Plant
Production Systems group, Wageningen University, has an
engineer degree in animal sciences at the same university (1989).
Thesis topics were the performance of different livestock species
for draught power, and improving the quality of rice straw as cattle
feed through treatment with urea. After working for 10 years in
West Africa, she received her PhD degree from the same university in 2000 on
“Mixed farming Systems in West Africa” amongst others focusing on nutrient and
financial budgets for different combinations of grazing and crop residue feeding at
farm and village level. Ever since she has investigated crop livestock systems
varying from fish-vegetable combinations in aquaponics in Ethiopia, soybean-
maize-chicken integration in Tanzania to oil palm cattle or goat integration in
Indonesia and Malaysia. A focus is on optimizing resource use benefitting both
livestock and crops.

Since more than 30 years she does research in smallholder systems, but also with
commercial enterprises, mostly in interdisciplinary research projects, which she
often co-designs and co-leads. Topics vary from agriculture providing
micronutrients for human health, competing claims on land by food/feed/fuel/nature,
sustainable cocoa production systems, living income.

About 15 years’ ago she started research on farming systems in oil palm. Topics
vary from factors affecting yield gaps (Woittiez et al., 2017), the use of the
PALMSIM to calculate yield gaps affected by climate (Monzon et al, 2021, 2022),
use of WanulCas to assess oil palm intercropping (Khasanah et al., 2020), oil palm
weeds as cattle feed (Grinnell et al., 2022), oil palm intensification (Special issue
Agric systems 2023), barriers for certification and for implementing better
management practices (Hutabarat et al., 2018; De Vos, 2019). She works with
RSPO, civil society organizations, policy makers, oil palm companies and research
institutes in Indonesia, Colombia, and the Netherlands. She is currently leading the
work on oil palm livestock integration and oil palm intercropping in
SUSTAINPALM program together with IPB and ULM, and will lead the new
OILCLIPS program on developing management for oil palm diseases under
changing climate and agricultural practices with UGM-Indsonesia and
CENIPALMA-Colombia. Together with animal production systems Wageningen
University and UGM she is currently supervising an Indonesian PhD researcher who
will research oil palm cattle integration from animal production, nutrient cycling and
disease control perspective.

She developed and teaches Global Food Security and the Advanced Bioresources
course and gives many guest lectures. She presents her work regularly in
international scientific conferences but also in multistakeholder meetings as WU
motto is “Science for Impact”.

10 | International Symposium on Innovative Approaches to Livestock Industry 2025

H-index 33 Publications 258 of which 70 in International top journals (Google
Scholar).

Prof. Sang Kee Kang

Maja Slingerland, currently associate professor at Plant Production Systems group,
Wageningen University, has an engineer degree in animal sciences at the same
university (1989). Thesis topics were the performance of different livestock species
for draught power, and improving the quality of rice straw as cattle feed through
treatment with urea. After working for 10 years in West Africa, she received her
PhD degree from the same university in 2000 on “Mixed farming Systems in West
Africa” amongst others focusing on nutrient and financial budgets for different
combinations of grazing and crop residue feeding at farm and village level. Ever
since she has investigated crop livestock systems varying from fish-vegetable
combinations in aquaponics in Ethiopia, soybean-maize-chicken integration in
Tanzania to oil palm cattle or goat integration in Indonesia and Malaysia. A focus is
on optimizing resource use benefitting both livestock and crops.

Since more than 30 years she does research in smallholder systems, but also with
commercial enterprises, mostly in interdisciplinary research projects, which she
often co-designs and co-leads. Topics vary from agriculture providing
micronutrients for human health, competing claims on land by food/feed/fuel/nature,
sustainable cocoa production systems, living income.

About 15 years’ ago she started research on farming systems in oil palm. Topics
vary from factors affecting yield gaps (Woittiez et al., 2017), the use of the
PALMSIM to calculate yield gaps affected by climate (Monzon et al, 2021, 2022),
use of WanulCas to assess oil palm intercropping (Khasanah et al., 2020), oil palm
weeds as cattle feed (Grinnell et al., 2022), oil palm intensification (Special issue
Agric systems 2023), barriers for certification and for implementing better
management practices (Hutabarat et al., 2018; De Vos, 2019). She works with
RSPO, civil society organizations, policy makers, oil palm companies and research
institutes in Indonesia, Colombia, and the Netherlands. She is currently leading the
work on oil palm livestock integration and oil palm intercropping in
SUSTAINPALM program together with IPB and ULM, and will lead the new
OILCLIPS program on developing management for oil palm diseases under
changing climate and agricultural practices with UGM-Indsonesia and
CENIPALMA-Colombia. Together with animal production systems Wageningen
University and UGM she is currently supervising an Indonesian PhD researcher who
will research oil palm cattle integration from animal production, nutrient cycling and
disease control perspective.

She developed and teaches Global Food Security and the Advanced Bioresources
course and gives many guest lectures. She presents her work regularly in
international scientific conferences but also in multistakeholder meetings as WU
motto is “Science for Impact”.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 11

 H-index 33 Publications 258 of which 70 in International top
journals (Google Scholar).

Prof. Dr. Despal, S.Pt. MSc.Agr

Prof. Dr. Despal, S.Pt, M.Sc.Agr is an experienced
researcher, lecturer, and practitioner in the field of tropical
dairy cattle, with a career spanning over three decades. Since
1994, she has been dedicated to advancing the science of
animal nutrition and feed technology, currently serving as a faculty member at the
Department of Animal Nutrition and Feed Technology, Faculty of Animal Science,
Bogor Agricultural University (IPB University).
She holds a Bachelor’s degree in Animal Production from IPB University, a
Master’s in Integrated Tropical Agriculture from Georg-August University in
Germany, and a PhD in Animal Nutrition & Physiology, also from Georg-August
University. Prof. Dr. Despal’s research interests are focused on several areas within
dairy cattle management, including the development of concentrate standards,
forage conservation, and local feed databases. She has made significant
contributions to ration formulation systems, functional milk production rich in CLA,
and techniques for fat supplementation in dairy cattle. Additionally, she has worked
on developing local feed NIRS databases and improving the effective fiber for dairy
cattle, along with advancing the knowledge on DCAD feed information and anionic
salt production for optimal dairy production.
With 45 Scopus-indexed publications and an H-index of 11, Prof. Dr. Despal has
been recognized for his scholarly contributions, accumulating a total Sinta score of
1797. He has also published nine national journal articles since 2000 and authored
four books, with one co-authored. In terms of intellectual property, she holds two
copyrights and one registered simple patent. Prof. Dr. Despal has also served as an
editor and reviewer for several reputable journals, including Bulmater, INTP IPB,
Tropical Animal Science Journal, American Journal of Animal and Veterinary
Science, Agripet, Unsyiah, and Buletin Peternakan, UGM.
Her career at IPB University began in 1996, and she has taken on multiple
leadership roles, including Manager of the Integrated Laboratory since 2020 and
Head of the Division of Dairy Nutrition since the same year. She has also
contributed as an Internal Auditor at IPB, Secretary of the Faculty of Animal
Science, and Consultant for sustainable dairy development projects, including a role
with SNV Netherlands. Her professional journey includes serving as an International
Collaboration Coordinator and Community Service Coordinator at IPB, as well as
consulting for dairy cattle development projects with PT Danone Indonesia and
contributing to national livestock agribusiness development as a judge for the
Directorate General of Livestock in Indonesia.

12 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Prof. Dr. Despal's dedication to his profession has been
recognized through numerous accolades, including the Satya
Lencana awards for 10 and 20 years of service and the highest
EPBM (Educational Performance-Based Management)
achievement within the Department of Animal Nutrition and
Feed Technology in 2013, 2014, and 2019.
Prof. Dr. Ir. Nahrowi, M.Sc
Prof. Nahrowi is a professor of nutrition and feed technology at
IPB University and a President of Center for Tropical Animal Studies. Nahrowi
graduated from IPB University with a Bachelor of Nutrition and Feed Science in
1995, a Master degree from Upsala University (Sweden) with Nutrition in 1990 and
Doctorate degree from University of Ehime (Japan in 1995). He was focused on
Research and Development about Potential Local Feedstuff and give some touch of
Technology to improve the quality and availability of Feedstuff. One of the
invention in potential feedstuff is Palm Kernel Meal-Hydrolisis as known as
PalmOfeed. He optimize the use of PKM-H in poultry until 12.5% in ration. He also
has achievment as Most Prospective Indonesian Innovations of 2017, 2013 and 2010
from Business Innovation Center (BIC) and the Indonesian Ministry of Research,
Technology and Higher Education.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 13

 Prof. drh. Arief Boediono, PhD PAVet
Doctoral education was completed at the United Graduate
School of Veterinary Science, Yamaguchi University Japan
in 1996. As one of the pioneers in the development of
fertility clinics in Fukuoka, Japan, experience and
knowledge gained after taking Post-Doctoral Fellow in Canada (University of
British Columbia), and the USA (Louisiana State University). At present as the
Head of the Embryology Laboratory at the School of Veterinary Medicine and
Biomedical Sciences, IPB University. Currently responsible as Scientific Director at
Morula IVF Indonesia, and President of the Indonesian Society of Human
Embryology (ISHE).
The professional organizations that actively participated were: Asia Pacific Initiative
on Reproduction (ASPIRE), Indonesian Association for In Vitro Fertilization
(IAIVF), Indonesian Stem Cell Association (ASPI), Indonesian Veterinary Medical
Association (IVMA), Indonesian Anatomists Association (IAA).
His scientific fields are: embryo production, gamete and embryo manipulation,
gamete and embryo cryopreservation, assisted reproductive technology (ART), and
stem cells.

14 | International Symposium on Innovative Approaches to Livestock Industry 2025

Prof. Gap-don Kim

Dr. drh. Okti Nadia Putri, M.Si, M.Sc

Prof. Dewi Apri Astuti

Doctor Dewi Apri Astuti is a Professor in physiological of nutrition at
the Department of Animal Nutrition and Feed Technology, Faculty of
Animal Science, IPB University, Indonesia. She graduated as
bachelor from Gadjah Mada University, Yogyakarta Indonesia
(1984) and received Master in Animal Nutrition (1988) and Doctor in
Physiological of Nutrition (1995) from IPB University, Bogor,
Indonesia. She has authored and co-authored for more than 300
publications in journals and proceedings, including 13 books: Monograph Cricket
Farming in Indonesia – Lambert (2016) and BSF for feed (2022). Research interests
in the field of ruminant nutrition with emphasis on nutrition by using insects for
functional diets. The publication entitled “BSF for ruminant” (Bioscience journal,
2022) is cited by many researchers. Some research collaborations was done with
National Institute of Animal Industry- Tsukuba, Japan (1998) and Hohenheim
University, Germany (2004), Wake Forest University , USA (2012-2013), Thunghai
University Taiwan (2018-2019) and Universiti Putra Malaysia (2020 - 2024). Now
she is a head of the Division of Meat and Draught Animal Nutrition, Coordinator of
Post Graduate Study Program at the Faculty of Animal Science IPB University,
member of ACUC at IPB University, A country representative for Indonesia in
Asian-Australasian Dairy Goat Network (AADGN, 2012-2014 and 2014-2016) and
continued as AADGN steering committee 2016-up to now. She got a National Gold
Academic Leader for agriculture science at 2024.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 15

Prof. Dr. Asep Gunawan, S.Pt, MSc
Current Position:
Professor
Department of Agricultural Biotechnology,
College of Agriculture and Life Sciences,
Seoul National University, Seoul, Republic of Korea
Email [email protected]

Education and careers:

2013.02 - Present: Professor at Seoul National University, Seoul, Republic of Korea
1992-2013.01: Professor at Chonnam National University, Gwangju
2005-2006: Visiting Professor, Michigan State University with Mike VandeHaar,
USA
1991-1992: Postdoctoral training with Dr. L. Hennighausen; NIH, USA
1988-1991: Ph. D. with professor C.S. Park in Animal Sciences, North Dakota State
Univ., Fargo, USA
1986-1988: M.S. in Animal Science, Seoul National University, Korea
1979-1986: B.S. in Animal Science, Seoul National University, Korea

Professional affiliations/Societies:
President, the Korean Society of Rumen Function Studies (2018 - present)
President, Korean Society of Animal Nutrition & Feed Technology Studies (2019 -
2021)

Editorial member:
Associate Editor, Animal Bioscience (2017- present)

Research area:
 Nutrigenomics on beef production
 Studies on development of feeding/management systems for improvement
of beef quality
 Ruminant stress mitigation and welfare
 Rumen microbiome and methane mitigation

Recent selected publications in Journal (2017 - )

1. Fassah, D. M., … & Baik, M. (2023). Effects of dietary glycerol supplementation
on meat quality, palatability, and lipid metabolism gene expression in the
longissimus thoracis of Hanwoo steers. Meat science, 198, 109093.
2. Jung, D.J.S., … & Baik, M. (2023) Effects of vitamin E and selenium
administration on transportation stress in pregnant dairy heifers. J Dairy Sci.
106:9576–9586
3. Park, S. J., … & Baik, M. (2023). Effects of genomic estimated breeding value and
dietary energy to protein ratio on growth performance, carcass trait, and lipogenic
gene expression in Hanwoo steer. Animal, 17(4), 100728.
4. Sang Weon Na , …, Myunggi Baik. (2021) Pseudoprevotella muciniphila gen.
nov., sp. nov., a mucin-degrading bacterium attached to the bovine rumen

16 | International Symposium on Innovative Approaches to Livestock Industry 2025

 epithelium. PLoS One. 20;16(5):e0251791
5. Seok-Hyeon Beak, …, Myunggi Baik. (2021) Relationships among carcass traits,
auction price, and image analysis traits of marbling characteristics in Korean cattle
beef. Meat Sci. 171(2021):108268.
6. Hyeok Joong Kang, …, Myunggi Baik. (2020) Effects of cold temperature and fat
supplementation on growth performance and rumen and blood parameters in early
fattening stage of Korean cattle steers. Anim. Feed Sci. Technol.
269(2020):114624.
7. Da Jin Sol Jung & Myunggi Baik. (2019) Up-regulation of bone morphogenetic
protein and its signaling molecules following castration of bulls and their
association with intramuscular fat content in Korean cattle. Scientific Reports
19807 (2019).
8. Park SJ, Kang HJ, Na S, Lee SH, Baik M. (2018) Differential expression of
extracellular matrix and integrin genes in the longissimus thoracis between bulls
and steers and their association with intramuscular fat contents. Meat Sci. 136:35-
43.
9. Baik M., et al. (2017) TRIENNIAL GROWTH AND DEVELOPMENT
SYMPOSIUM: Molecular mechanisms related to bovine intramuscular fat
deposition in the longissimus muscle. J Animal Science. 95(5):2284-2303.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 17

18 | International Symposium on Innovative Approaches to Livestock Industry 2025
 PLENARY SESSION (FIRST DAY)

Time Title Moderator

10.15-11.00 Methane Reduction Strategies in Livestock for Dr. Idat G. Permana
Sustainable Agriculture
Kebreab, E.
11.00-11.45 Challenges to Food Security Stemming from Prof. Dr. Asep
Animal Health Gunawan
M. J. Uddin, E. A. Rana, M. Akhter, M. A.
Rahman, and S. H. Akter
12.45-13.30 Quality and Quantity of Plantation Undergrowth Dr. Windi Al Zahra
in Integrated Cattle-Oil Palm Production Systems
Shekhar Chettri, Aart van der Linden,
Muhammad Fahmi Sidiq, Wahyu Darsono, Ika
Sumantri, Maja Slingerland
13.30-14.15 The Miracle of Black Soldier Fly: from Research Dr. Windi Al Zahra
to Table.
Dewi Apri Astuti

International Symposium on Innovative Approaches to Livestock Industry 2025 | 19

Methane Reduction Strategies in Livestock for Sustainable
Agriculture

Kebreab1, E.
1
Department of Animal Science, College of Agricultural and
Environmental Sciences, University of California, Davis, Davis,
CA, 95616, USA

E-mail correspondence: [email protected]

Abstract. Livestock production is a major contributor to methane

emissions, a potent greenhouse gas that significantly impacts global
climate change. This paper presents a comprehensive overview of
cutting-edge strategies aimed at reducing methane emissions from
livestock systems, essential for meeting global climate targets. The
study highlights the efficacy of feed additives, particularly
bromoform-containing seaweed, which has demonstrated up to 98%
effectiveness in mitigating methane emissions. In addition, the
potential of genetic selection for breeding low-methane-emitting
livestock is explored, revealing reductions of 11-26% in emissions.
Another recent development is microbial engineering, utilizing
CRISPR technology to edit rumen microbiomes, offering a long-
term, scalable solution to methane reduction. Furthermore, the
integration of these strategies with productivity enhancements,
especially in low-income regions, is discussed as a means to reduce
methane intensity without increasing livestock numbers. The
findings underscore the importance of a multipronged approach,
combining short-term and long-term solutions, to significantly
mitigate the environmental impact of livestock production. These
strategies are crucial for aligning livestock systems with global
sustainability goals, ensuring that agricultural practices contribute
positively to the climate agenda.

Keywords: Methane, Feed additives, Genetic selection, Microbial

engineering, Climate Change.

20 | International Symposium on Innovative Approaches to Livestock Industry 2025

Challenges to Food Security Stemming from Animal Health

M. J. Uddin1,2,3, E. A. Rana1, M. Akhter1, M. A. Rahman1, and S.

H. Akter1
1
School of Veterinary Medicine, Murdoch University, WA 6150,
Australia
2
Centre for Biosecurity and One Health, Harry Butler Institute,
Murdoch University, WA 6150, Australia
3
Centre for Animal Production and Health, Food Future Institute,
Murdoch University, WA 6150, Australia

E-mail correspondence: [email protected]

Abstract. The global demand for animal-derived foods has

dramatically increased in the past decades and is expected to continue
rising significantly in the foreseeable future. Animals are being bred
for higher production and improved performance, which challenges
their physiology and makes them more vulnerable to various diseases.
As a result, traits related to disease resilience are becoming
increasingly important to incorporate into breeding plans.
Furthermore, disease outbreaks have become more frequent in recent
years due to human activity, ecosystem disruption, and climate
change. Animal health problems are threatening animal-derived food
security globally. Researchers are at the forefront of addressing food
security challenges through advanced technologies, including the
development of rapid and sensitive pen-site tests for early disease
diagnosis. The application of high-throughput genomic techniques
plays a pivotal role in disease diagnosis, tracking, control, and
preparedness strategies. Early and accurate diagnosis enables
appropriate treatment planning, including reducing the use and
reliance on antimicrobials, thereby combating antimicrobial
resistance. Additionally, sustainable approaches, such as the use of
naturally abundant bacteriophages, are also gaining attention. Vaccine
development and it’s application in livestock play a crucial role in
protecting animals from preventable diseases and safeguarding against
economic losses. These efforts are crucial in containing zoonotic
diseases, minimizing spillover, and combating antimicrobial resistance

International Symposium on Innovative Approaches to Livestock Industry 2025 | 21

 through a One Health approach, which ultimately ensures food
security. Global collaboration among animal scientists, veterinarians,
and infectious disease specialists will enhance both animal and public
health, contributing to greater food security.

Keywords: Food security, Animal health, Disease resilience,

Infectious disease outbreaks, Diagnosis, Vaccine

Quality and Quantity of Plantation Undergrowth in

Integrated Cattle-Oil Palm Production Systems

Shekhar Chettri1,2, Aart van der Linden2, Muhammad Fahmi

Sidiq1, Wahyu Darsono3, Ika Sumantri4, Maja Slingerland1
1
Plant Production Systems, Wageningen University, P.O. Box 430,
6700AK Wageningen, The Netherlands
2
Animal production Systems, Wagreningen University, P.O. Box
338, 6700AH Wageningen, The Netherlands
3
Siska ranch, PT Buana Karya Bhakti, South Kalimantan, Indonesia
4
Department of Animal Sciences, Universitas Lambung Mangkurat,
Banjarbaru, Indonesia

E-mail correspondence: [email protected]

Abstract. In Indonesia integrating cattle and oil palm production

supports local beef supply, increases plantation income, and saves
land. This study assesses effects of palm age and selection pressure
on the species composition, quality, and quantity of the undergrowth
and its carrying capacity. Rotationally grazed and non-grazed
blocks, with oil palm monoculture planted in 2009 and 2012, were
sampled (May 2023). Biomass from quadrats (0.5 m 2) was sorted to
measure frequency and dry weight per plant species. The number of
species significantly declined with palm age, irrespective of grazing.
Average standing biomass was significantly higher in non-grazed
than in grazed blocks. For grazed blocks the average biomass in the
younger plantings was higher than in the older with 1230 vs 712 kg
DM per ha. Consumption data revealed that diet quality was higher
in younger versus older plantings with CP of 11.1 vs 10.2% and ME
of 6.2 vs 5.6 MJ/kg respectively. Cattle selected more of nutritious

22 | International Symposium on Innovative Approaches to Livestock Industry 2025

species such as Axonopus compressus compared to their availability.
Average diet quality was enough to support cattle production. When
palm age increases supplementary feed e.g. palm kernel meal or
lower stocking rates are advised to compensate for lower average
quality and quantity of undergrowth.

Keywords: cattle-oilpalm integration, undergrowth species, feed

quality, biomass.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 23

The Miracle of Black Soldier Fly: from Research to Table

Dewi Apri Astuti

Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Feed is the important factor to improve animal production

and reproduction. The quality, quantity, type and mode of action of
feed will affect to animal performance. Black soldier fly (BSF,
Hermetia illucens) is insect which has high quality chemical
composition, easy to produce and act as a bio-composter, can be
used as protein source and be promoted for fish meal substitution in
the ration. This review paper will explain the miracle of BSF as a
feed or food with technology approach to produce functional diet for
animal, snack for stunting children and also fertilizer to improve
agriculture products. A ten years study was done to evaluate quality
of BSF in different medium and to create such kind of milk replacer,
creep feed, herbal mineral blok (HMB), frass, fertilizer and also
some snack for stunting, based on BSF products. The quality of BSF
in different medium rearing were differ based on quality of the
media. Organic waste medium will produce high fat BSF,
meanwhile palm kernel meal or tofu waste have high protein BSF.
One of the miracle BSF is, they have high lauric acid in the total
BSF oil, means that seems to be a considerable material as an
alternative to improve the health and immune response and
antibiotic growth promoters (AGPs) since they are rich in
antimicrobial peptides (AMPs). The in vitro study by using BSF
larva meal in the ration showed that activity and phagocytosis
capacity of peritoneal macrophage to S. aureus and also antibody
titre to Avian Influenza virus quail were 89% and 9.13/cell and also
192, respectively, higher compared to the control ration without
BSF meal. Around 10% BSF larva in different type (fresh, dry and
BSF Me-OH extracted) in laying chicken ration resulted the best
performance was in BSF Me-OH extracted treatment, meanwhile

24 | International Symposium on Innovative Approaches to Livestock Industry 2025

the highest IOFC was in BSF fresh treatment. Utilization of BSF in
milk replacer formula for boerka and sapera kids showed that could
replace goat milk without any problem in palatability and could
increase ADG around 106-112 g/h/d. In small ruminant growing
animal with different breed giving by creep feed containing BSF
meal showed very good data on ADG, where for kacang, etawah
crossbred, senduro and dorper crossbred goat have 90, 120, 160 and
230 g/h/d, respectively. Feeding HMB based on BSF meal giving to
growing and lactating dorper sheep showed they did not have
significance difference in performance, except in the serum-Ca
higher than control. In Indonesia stunting program with necessary
need high protein and vitamin A in the diet, so BSF meal could be
one alternative to be used in the stunting children snack for daily
consumption. The last but not least, BSF frass function in
agriculture could be a high fibre ration for growing or fattening
ruminant and also can be used for fertilizer in any agricultural
crops..

Keywords: Black soldier fly, Creep feed, herbal mineral block,

miracle, milk replacer.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 25

 PLENARY SESSION (SECOND DAY)

Time Title Moderator

08.30-09.15 Korean Cattle Industry and Nutrigenomics of Prof. Dr. Despal
Beef Quality
Myunggi Baik
09.15-10.00 Mitigation of Enteric Methane for Sustainable Prof. Dr. Despal
Ruminant Production
Satoshi Koike
10.15-11.00 Recombinant Protein Production in Silkworms Dr. Cahyo Budiman
Kazuhito Fujiyama, Hiroyuki Kajiura, and Ryo
Misaki

26 | International Symposium on Innovative Approaches to Livestock Industry 2025

Korean Cattle Industry and Nutrigenomics of Beef Quality

Myunggi Baik, Ph.D., Professor

Department of Agricultural Biotechnology, College of
Agriculture and Life Sciences, Seoul National University,
Seoul 08826, Republic of Korea

E-mail correspondence: [email protected]

Intramuscular fat (IMF) deposition or marbling in the longissimus

dorsi muscle is the most important trait affecting beef quality and
palatability, as well as beef price, particularly in Korean cattle
(Hanwoo). This presentation addresses Korean cattle industry and
beef carcass grading system in South Korea. Beef quality is
characterized by marbling, physicochemical, and sensory traits. This
presentation addresses beef-quality traits and recent research
progresses on the genetic, management, and nutritional factors that
affect beef quality. Mechanisms of IMF deposition with emphasis
on adipogenesis and lipogenesis are addressed. Selected signaling
pathways associated with IMF deposition are presented. This
information will be useful in the design of efficient methods to
improve beef quality. Nutrients regulate gene expression via
transcription factors, thereby affecting metabolism and the
phenotype (growth rate, beef quality, etc.). The control of gene
expression through several nutrients for IMF deposition is
addressed. The concept of genome-based precision feeding in
Korean cattle is presented.

Keywords: Korean cattle, beef quality, intramuscular fat,

nutrigenomics, genome-based precision feeding

International Symposium on Innovative Approaches to Livestock Industry 2025 | 27

Mitigation of Enteric Methane for Sustainable Ruminant
Production

Satoshi Koike
Research Faculty of Agriculture, Hokkaido University, Sapporo
060-8589, Japan

E-mail correspondence: [email protected]

Abstract. Ruminant animals harbor dense and diverse microbial

populations in their rumen. The rumen microbes ferment feed and
produce short chain fatty acids (SCFAs), the main energy source for
the host. Food security has become a major global concern as the
world population is increasing rapidly. Improvement of livestock
production efficiency with low adverse environmental impact is
required to ensure a stable supply of meat and milk, which are
important animal protein sources. Ruminant animals mainly depend
on rumen microbes to acquire nutrients from the feed, particularly
from forage. Therefore, modulation of rumen microbiota is one of
the options to improve the productivity of ruminant animals.
Although rumen fermentation enables ruminants to produce meat
and milk from the forages, a non-negligible amount of methane gas,
which has a 28 times higher potential of greenhouse effect compared
to carbon dioxide, is produced along with anaerobic fermentation in
the rumen. Therefore, efforts have been made to minimize methane
emissions from the rumen by regulating rumen fermentation via
dietary manipulation. Methane mitigation in the ruminant livestock
sector can contribute to easing the issues on the global environment
and food supply simultaneously if successfully proceeded.

Keywords: Cattle, Rumen, Methane, Microbiota, Feed additives.

28 | International Symposium on Innovative Approaches to Livestock Industry 2025

Recombinant Protein Production in Silkworms

Kazuhito Fujiyama1,2, Hiroyuki Kajiura1,2, and Ryo Misaki1,2

1
International Center for Biotechnology, Osaka University, 2-1
Yamada-oka, Suita, Osaka 565-0871, Japan
2
Institute for Open and Transdisciplinary Research Initiatives
(OTRI), Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871,
Japan

E-mail correspondence:[email protected]

Abstract. Bombyx mori was first domesticated silk moth. In these

days, silkworms are an attractive host for production of recombinant
proteins, such as enzymes, biopharmaceutical proteins and vaccines,
because the host can show high productivity and potential of
mammal-like post-translational modifications. For the recombinant
protein production, we apply transgenic silkworms or transient
expression in silkworms using baculovirus. In this presentation, I
will show technological developments for protein production in
transgenic silkworms.

Keywords: Recombinant protein, Silkworm, Glycosylation,

Transgenic, Transient expression

International Symposium on Innovative Approaches to Livestock Industry 2025 | 29

 ORAL PRESENTATION (FIRST DAY)

Sustainable animal genetic resources (OP-1)

Moderator: Amelia Kamila Islami, S.Pt., M.Si.
Room 1

Time Abs No. Title Author

15.30-15.45 OP-1-1 Asian Tropics Vs. African Tropics: Are P. K. Astuti, S.
Sheep Climate Adaptations Genetically Kusza
Different?
15.45-16.00 OP-1-2 Transcriptomic Study of IPB-D2 D. Lestari, A.
Chickens Cecal Tonsils with Different Gunawan, S.
Newcastle-Disease Antibody Titer Murtini, N. Ulupi,
I. Khaerunnisa, and
C. Sumantri
16.00-16.15 OP-1-3 The pivotal role of heat resistance R. R. Noor, Y. G.
genes in Bali cattle and its strategic M. Bonawati, I.
utilization in adapting to global Suhendro, and
warming Jakaria
16.15-16.30 OP-1-4 Fatty Acid Composition and Nutritional O. L. Bondoc
Indices/Ratios of Pork, Milk, and Eggs
from Local (Native) Breeds and
Hybrids in The Philippines
16.30-16.45 OP-1-5 Agro-Advocacy Organizations as P. Strausz, Cs.
Management Actors in the Process of Latorcai
Knowledge Transfer for Maintaining
Genetic Diversity in Husbandry – Best
Practices in the European Union
16.45-17.00 OP-1-6 Concepts, methods and experiences to Sz. Kusza, Z. Bagi,
avoid loss of animal genetic diversity and P.K. Astuti
under the challenges of climate change

30 | International Symposium on Innovative Approaches to Livestock Industry 2025

Asian Tropics Vs. African Tropics: Are Sheep Climate
Adaptations Genetically Different?

P. K. Astuti1,2,3, S. Kusza1,*
1
Centre for Agricultural Genomics and Biotechnology, University
of Debrecen, 4032 Hungary; [email protected],
2
Doctoral School of Animal Science, University of Debrecen,
Debrecen 4032 Hungary;
3
Department of Animal Breeding and Reproduction, Faculty of
Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281,
Indonesia;

E-mail correspondence: [email protected]

Abstract. The livestock farming sector is currently focusing on

adapting to hot/tropics climates to ensure survival in the era of climate
change. The tropics refer to the region encompassing both land and
sea surrounding the equator, lying between the Tropic of Cancer in the
north and the Tropic of Capricorn in the south. This region
encompasses approximately 15% of the Asian continent and 70% of
the African continent. Although countries in both continents are
classified under the same climate zone, their tropical characteristics
differ. Past studies have demonstrated that climatic factors, including
altitude precipitation, wind speed, and temperature, are essential in
determining the specific variation of adaption. To maintain the long-
term sustainability of livestock production in a changing climate, it is
crucial to comprehend the biological processes involved in adapting to
various tropical climate zones. Genomic methods can identify
genomic regions and genes responsible for heat resistance in tropical
sheep breeds. Our research project aims to employ a medium density
SNPs chip to analyse the compare the genetics of Indigenous sheep
from Asian and African tropics. This study may aid the genetic
mapping of indigenous sheep breeds from different continents that
have adapted to tropical climes, particularly in thermoregulation and
heat tolerance.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 31

 Keywords: Climate adaptation, Genomics, Heat stress, Ovis aries,
Tropics.

Acknowledgement: The research is supported by the EKÖP-24-4 University

Research Scholarship Program of the Ministry for Culture and Innovation from the
source of the National Research, Development and Innovation Fund and this
conference participation was supported by University of Debrecen Program for
Scientific Publication. Thanks to Stipendium Hungaricum Scholarship from
Tempus Public Foundation for supporting PKA studies.

32 | International Symposium on Innovative Approaches to Livestock Industry 2025

Unveiling the Genetic Potential of Newcastle Disease
Resistance in IPB-D2 Chickens: A Transcriptomic Approach
for the Sustainability of Indonesian Chicken Genetic
Resources

D. Lestari1, A. Gunawan2, S. Murtini3, N. Ulupi2, I.

Khaerunnisa4 and C. Sumantri2
1
Research Center for Applied Zoology, National Research and
Innovation Agency, Bogor, 16911, Indonesia
2
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Bogor, 16680, Indonesia
3
Division Medical Microbiology, School of Veterinary Medicine
and Biomedical Science, IPB University, Bogor, 16680, Indonesia
4
Research Center for Applied Zoology, National Research and
Innovation Agency, Bogor, 16911, Indonesia

E-mail correspondence: [email protected]

Abstract. Disease resistance is influenced by many genes.

Improving the quality of disease resistance, especially in local
Indonesian chickens through transcriptomic analysis through RNA-
Seq analysis has not been done much. The aim of this study was to
conduct preliminary research on the genes that contribute to high
and low ND antibody titers in IPBD2 chickens. A transcriptomic
analysis based on RNA-Seq of cecal tonsils was performed in IPB-
D2 chicken with high and low Newcastle Disease antibody titer. In
total, 1.680 genes were differentially expressed included
upregulated and downregulated genes. Analysis showed that MAPK
signaling pathway as the dominant pathway behind the high and low
ND antibody titer in IPB-D2 chicken. Potential genes were
categorized into 3 categories i.e., biological process, cellular
components, and moleculer function. PPI analysis showed 10 genes
with highest topology score i.e., GSK3A, CDC42BPA, UTRN,
MECOM, SYNE2, CDH23, SMURF1, CD44, ZBTB38, and
FGRF3. Based on several analyses, there are 5 potential genes that

International Symposium on Innovative Approaches to Livestock Industry 2025 | 33

 affect the high and low titer of ND antibodies in IPB-D2 chickens,
namely ZBTB38, CDC42BPA, CD44, TLR2A and CCR9.

Keywords: Cecal tonsils, IPB-D2 chicken, Newcastle-Disease

antibody titer, Transcriptome

34 | International Symposium on Innovative Approaches to Livestock Industry 2025

The Pivotal Role of Heat Resistance Genes in Bali Cattle and
Its Strategic Utilization in Adapting to Global Warming

R. R. Noor1*, Y. G. M. Bonawati1, I. Suhendro2, and J. Jakaria1

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
National Research and Innovation Agency, Cibinong, Bogor,
Indonesia

E-mail correspondence: [email protected]

Abstract. One of the steps taken is to analyze and utilize the

primary genes related to body temperature homeostasis, which
directly impacts livestock resistance to heat. This study aimed to
profile the HSP70 gene in Bali cattle of 12 Bali cattle from UPTD
Sobangan, Gianyar Regency, Bali Province, and UPTD Kupang
Regency, NTT using sequencing techniques to obtain specific SNPs
and analyze their expression in different maintenance systems
(intensive and extensive). The results of the long sequence analysis
of the HSP 70 gene in the 1926 bp coding region (ATGTAG)
showed a 31 bp indel in the 5'UTR region and 6 SNPs in the coding
region with a total of 5 SNPs (c.90 C/T, c.156A/C, c.279 C/T,
c.1446 C/T, c.1569 A/G, c.1596 G/A) synonymous and one SNP
c.1800 G/C non-synonymous which causes a change in amino acids
from glutamic acid (E) to aspartic acid (D) with a total of 641 amino
acids. The expression of the HSP70 gene in Bali cattle that were
maintained in an intensive and extensive environment showed no
significant difference. The study proves that Bali cattle can adapt to
different maintenance system conditions.

Keywords: Bali cattle, HSP70 gene, SNP, gene expression, global

warming.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 35

Fatty Acid Composition and Nutritional Indices/Ratios of
Pork, Milk, and Eggs from Local (Native) Breeds and Hybrids
in The Philippines: A Review

O. L. Bondoc, Ph.D. 1
1
Professor, Animal Breeding Division, Institute of Animal Science,
College of Agriculture and Food Science, University of the
Philippines Los Baños, Laguna 4031 Philippines

E-mail correspondence: [email protected]

Abstract. Fats and fatty acids (FAs) in meat, milk, and eggs
produced by local (native) breeds and hybrids are important
nutritional components that may have different nutritional qualities
and effects on human health. This paper highlights the FA
composition and nutritional indices of pork ham from Black Tiaong
native pigs, milk from “Murrah × Carabao” crossbred buffaloes,
and egg yolk from Joloano native chickens and balut produced by
Itik-Pinas Kayumanggi mallard ducks. The major FAs with highest
proportions by weight of total FAs in pork, milk, and egg yolk are
palmitic acid–C16:0, stearic acid–C18:0, and oleic acid–C18:1n-9.
Lauric acid–C12:0 and myristic acid–C14:0 are major saturated FAs
in milk. Linoleic acid–C18:2n-6 is a major polyunsaturated FA in
pork and egg yolk. SFAs were lowest in pork fats and highest in
milk fats. Yolk fats from chickens seem to have more health benefits
because of highest PUFA-to-SFA ratio and hypocholesterolemic-to-
hypercholesterolemic ratio. Yolk fats from balut had highest
monounsaturated FA-to-SFA ratio and health-promoting index
value; and lowest atherogenicity and thrombogenicity. These
findings suggest the importance of native animal genetic resources
to provide nutritional security in rural areas with limited or no
access to pork, milk, and eggs produced commercially.

Keywords: Egg yolk, fatty acid, local breeds, milk, nutritional

indices, pork.

36 | International Symposium on Innovative Approaches to Livestock Industry 2025

Agro-Advocacy Organizations as Management Actors in The
Process of Knowledge Transfer for Maintaining Genetic
Diversity in Husbandry – Best Practices in The European
Union

P. Strausz1* and Cs. Latorcai2

1
Department of Management, Institute of Strategy and management,
Corvinus University of Budapest, Budapest, 1093, Hungary
2
Department of Humanities and Vocational Education, Institute of
Rural Development and Sustainable Economy, Hungarian
University of Agriculture and Life Sciences, Gödöllő, 2100,
Hungary

E-mail correspondence: [email protected]

Abstract. Over the past decade, climate change has made the shift
towards sustainable farming even more urgent. Closely related to
this phenomenon is the need to maintain genetic diversity, which on
the one hand offers the opportunity to put local breeds capable of
adapting to the changing climate into production or to create new
breeds, and on the other hand helps to preserve the animal genetic
treasures and values of a given country or region. It requires the
joint work of science, the respective state and breeders. In many
regions, however, organizations in the mezzosphere of economics
and society that could play an important mediating role in
disseminating new scientific results, recognizing their importance
and later applying them in practice are absent or still inexperienced.
The presentation aims to present those European best practices that
exist at Member State and Community (European Union) level,
primarily by agrarian interest representatives (chambers, breeders'
organisations). In addition to describing the goals, membership
system and main activities of these organizations, the presentation
pays special attention to their efforts in knowledge transfer, active
contact with breeding members, during the educational work, the
management and communication strategies and tools used.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 37

 Keywords: agro advocacy, climate change, future directions and
innovation, genetic diversity, husbandry, management,
sustainability.

38 | International Symposium on Innovative Approaches to Livestock Industry 2025

Concepts, Methods and Experiences to Avoid Loss of Animal
Genetic Diversity Under the Challenges of Climate Change

Sz. Kusza1*, Z. Bagi1, and P. K. Astuti1

1
Centre for Agricultural Genomics and Biotechnology, Faculty of
Agricultural and Food Sciences and Environmental Management,
University of Debrecen, Debrecen, 4032, Hungary

E-mail correspondence: [email protected]

Abstract. In the face of climate change, maintaining genetic

diversity in livestock and wild animals is vital for ensuring
resilience and adaptability. This presentation aims to focus on the
importance, aims of genetic conservation work and its method, and
its role in maintaining genetic diversity to enhance mainly livestock
resilience. Genomic analysis and population genetics tools were
used to assess the genetic diversity of several animal breeds across
different climatic regions. Our results indicate that high genetic
diversity correlates with improved adaptability to environmental
stressors. The outcomes of our studies emphasize the need for
ongoing genetic conservation initiatives to protect livestock and
wild animals’ biodiversity, which is essential for long-term
sustainability. In conclusion, the preservation of genetic diversity in
animals not only bolsters the resilience of these species but also
supports broader ecological and agricultural stability in a rapidly
changing climate.

Keywords: climate change, conservation; genetic diversity loss,

genomic studies, livestock, sustainability, wild animals.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 39

40 | International Symposium on Innovative Approaches to Livestock Industry 2025
Smart animal production systems and management (OP-2)
Moderator: Dr. Isyana Khaerunnisa
Room 2

Time Abs No. Title Author

15.30-15.45 OP-2-1 Feeding Dietary Fiber Source in Post- D. Pamungkas, L.
Partum Crossbred Cows Affandhy, Aryogi,
M. Luthfi, M.
Chanafi, R. Antari,
M. Primananda,
W.I. Wulansari, and
Mudawamah
15.45-16.00 OP-2-2 Impact of Temperature-Humidity Index Mazlishah, M.S.H,
(THI) on the Physiological and Sperm Mohamad-Fauzi,
Quality of Barbados Blackbelly Sheep N., Mohd Nor, M.
F. F., & Hashida,
N.H.
16.00-16.15 OP-2-3 Development Strategy of Dairy Farmer Y. L. Purnamadewi,
Cooperatives in Optimizing Services to A. Asmara, and D.
Members and Supporting Food Tanjung
Security in The Industrial Era 4.0
16.15-16.30 OP-2-4 Reproductive Performance of Pigs in Marchie. A. Da
Local Communities on Ambon Island Costa, P. R.
towards Sustainable Livestock Business Matitaputty, E.
Waas, S. S.
Kaihatu, M.
Nurdin, T. N.
Ralahalu, and D. K.
Hau
16.30-16.45 OP-2-5 Perfomance Production of Ongole EL Aditia, R.
Grade Heifers during Recondition Priyanto, L
Period Fed with Concentrate and Abdullah, PDMH
Fermented Feed Karti, and T
Milliyana

International Symposium on Innovative Approaches to Livestock Industry 2025 | 41

Feeding Dietary Fiber Source in Post-Partum Simmental
Crossed Cows

D. Pamungkas1, L. Affandhy1*, Aryogi1, M. Luthfi1, M.

Chanafi11, R. Antari1, M. Primananda2, W. I. Wulansari1, and
Mudawamah3

1
Research Center of Animal Husbandry, Research Organization
Agricultural and Food, National Agency for Research and
Innovation, Republic of Indonesia (BRIN)
2 Large Ruminant Instrument Standardization Center, Ministry of
Agriculture, Grati, Pasuruan, East Java, Indonesia.
3 Malang Islamic University, Malang, East-Java, Indonesia

E-mail correspondence: [email protected]

Abstract. The aim of this study was to evaluate the reproductive

performance of postpartum Simmental crossed cows on a high-fiber
diet inclusion, including rice straw (RS), elephant grass (EG), and
sorghum leaf (SL). As of 24 cows were divided into four feeding
regimes: T1 (25 kg SL + 10 kg EG), T2 (20 kg SL + 10 kg EG), T3
(15 kg SL + 10 kg EG), and T4 (10 kg SL + 10 kg EG), with RS
provided ad libitum. These diets were administered three months
post-calving. Artificial insemination was conducted around 45 days
after calving. The study used a completely randomized design with a
one-way ANOVA. Results showed that dry matter intake was the
highest in T1 (5.9 kg/d), followed by T2 (5.3 kg/d), T3 (4.6 kg/d),
and T4 (3.9 kg/d). Similar trends were observed for crude protein
and fiber intake. Calf birth weights ranged from 34.8 to 36.0 kg,
with daily gains of 0.4 to 0.6 kg/d. No significant differences were
found in Anestrus postpartum, Services per Conception, and
Days Open. The highest Conception Rate (85.7%) was in T1. Thus,
dietary fiber sources significantly affected nutrient intake but did not
adversely impact the reproductive performance.

42 | International Symposium on Innovative Approaches to Livestock Industry 2025

Keywords: feeding, dietary fiber, post-partum, reproductive
performance, Simmental crossed cows.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 43

Impact of Temperature-Humidity Index (THI) on the
Physiological and Sperm Quality of Barbados Blackbelly
Sheep

M. S. H. Mazlishah1,2, N. Mohamad-Fauzi1,2, M. F. F. Mohd

Noor3, and N. H. Hashida1,2*
1
Glami Lemi Biotechnology Research Centre, Universiti Malaya,
71650 Jelebu, Negeri Sembilan, Malaysia
2
Institute of Biological Sciences, Faculty of Science, Universiti
Malaya, 50603 Kuala Lumpur, Malaysia
3
Institute of Ocean and Earth Sciences, Universiti Malaya, 50603
Kuala Lumpur, Malaysia

E-mail correspondence: [email protected]

Abstract. Climate change leads to heat stress occurrence in small

ruminants, results in critical effects on farm productivity, animal
welfare, productivity, and subsequently leads to significant
economic losses. Sixteen Barbados Blackbelly rams were exposed
to the intensive (ISM) and semi-intensive management (SIM)
systems (open grazing space, 6 hours/day) for 2 months. An in-
house weather station and a temperature-humidity logger were used
to measure Temperature-Humidity Index (THI). Weekly rectal
temperature, respiration rate, heart rate, and testicular temperature
were measured. Semen was evaluated using Computerized Assisted
Sperm Analyzer (CASA) for concentration, motility,
progressiveness, Path Velocity (VAP), Progressive Velocity (VSL)
and velocity distribution.The findings showed that THI would have
an impact on the management system and cause heat stress in rams.
Generally, THI of SIM was significantly greater than ISM system.
The semi-intensive rams recorded significantly higher rectal
temperatures, heart rates, and respiration rates than ISM which
indicating heat stress. However, the rams from ISM showed
significantly higher sperm motility and progressiveness than the
SIM. In conclusion, the SIM system coud expose animals to higher
THI, therefore affects reproductive performance of ram. Effective

44 | International Symposium on Innovative Approaches to Livestock Industry 2025

management strategies are necessary to mitigate its impact, ensuring
the sustainability and profitability of small ruminant farming.

Keywords: barbados blackbelly sheep, management systems,

physiological assessment, sperm quality, temperature humidity
index (THI).

International Symposium on Innovative Approaches to Livestock Industry 2025 | 45

Development Strategy of Dairy Farmer Cooperatives in
Optimizing Services to Members and Supporting Food
Security in The Industrial Era 4.0

Y. L. Purnamadewi1*, A. Asmara1, and D. Tanjung2

1
Department of Economics, Faculty of Economics and
Management, IPB University, Jl. Kamper Wing 4 Level 5, Dramaga
Campus, Bogor 16680, Indonesia
2
Study Program of Agribusiness, School of Vocasional Studies, IPB
University, Jl. Kumbang No.14, Cilibende Campus, Bogor 166,
Indonesia

E-mail correspondence: [email protected]

Abstract. The purpose of cooperatives is to improve the welfare of

members through the services they need. In the industrial era 4.0,
the capacity of cooperatives as business entities, both in relation to
internal members and with external parties in the upstream and
downstream industries, is determined by their ability to undergo
digital transformation. Digital transformation requires relatively
large investments; meanwhile, dairy farmer cooperatives (DFC) in
general have limited capital. The purpose of this research is to
formulate a digital-based cooperative development strategy for
optimizing services to members. The main data used is primary data
collected through interviews with 89 cooperative members
determined by stratified random sampling and also collected through
focus group discussions from stakeholders. The analysis method
used is the Analytic Network Process (ANP). The results of the
analysis show that there are five strategies that must be prioritized in
the development of DFC towards digital transformation in
optimizing services to members, namely increasing the cattle
population through the utilization of government programs and
equity capital; socio-economic improvement of members through
diversification of dairy products and diversification of output and
input markets; improvement of livestock health services and feed

46 | International Symposium on Innovative Approaches to Livestock Industry 2025

quality; partnerships in product innovation and digital platforms;
and digital transformation.

Keywords: ANP, cooperatives, dairy farmers, development

strategy, digital transformation.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 47

Reproductive Performance of Pigs in Local Communities on
Ambon Island towards Sustainable Livestock Business

M. A. Da Costa1, P. R. Matitaputty1*, E. D. Waas2, S. S.

Kaihatu2, M. Nurdin3, T. N. Ralahalu4, and D. K. Hau1
1
Research Center for Animal Husbandry, National Research and
Innovation Agency, Jl. raya Jakarta Bogor, Cibinong 16911, Indonesia
2
Research Center for Estate Crop, National Research and Innovation
Agency, Jl. Raya Jakarta Bogor, Cibinong 16911, Indonesia
3
Research Center for Behavioral and Circular Economics, National
Research and Innovation Agency, Jl. Gatot Subroto Jakarta 12710,
Indonesia
4
Department of Animal Husbandry, Faculty of Agriculture, Pattimura
University, Jl. Ir. M. Putuhena Poka, Ambon 97234, Indonesia

E-mail correspondence: [email protected]

Abstract. Reproductive performance is one of the main priorities in

the development of sustainable pig farming. Lack of knowledge of
farmers about reproduction can be an obstacle in future maintenance
management. This study aims to determine the reproductive
performance of pigs, as well as factors affecting business
sustainability. This study used survey methods and direct interviews
in the field using structured questionnaires. Respondents were 32
pig farmers from 3 villages, namely Latuhalat Village (11
respondents): Passo Village (9 respondents) and Waitatiri Village
(12 respondents). Parameters observed included: mating method,
lactation duration, dry period after lactation, and weaning age. All
data were analysed descriptively. The results showed that generally
farmers in the three sample villages conducted natural mating
method of pigs (100%); Length of lactation of piglets in Latuhalat
Village 60 days (81.82%), 25 days (18.18%); Passo Village 60 days
(100%); Waitatiri Village 60 days (91. Weaning age in Latuhalat
Village 60 days (81.82%), 25 days (18.18%); Passo Village 60 days
(100%); Waitatiri Village 60 days (91.67%) and 45 days (8.33%);
Dry period after lactation of the three sample villages Latuhalat,
Waitatiri, Passo is 30 days (100%). It is concluded that the

48 | International Symposium on Innovative Approaches to Livestock Industry 2025

 reproductive performance of pigs in the local community of Ambon
Island shows relatively uniform practices among farmers, but more
education and counselling is needed to improve reproductive
management that is still lacking to support the sustainability of pig
farming on Ambon Island.

Keywords: local community, reproduction, pig farming,

sustainability

Performance Production of Ongole Grade Heifers during

Recondition Period Fed with Concentrate and Fermented
Feed

E. L. Aditia1*, R. Priyanto1, L. Abdullah2, P. D. M. H. Karti2, and

T Milliyana1
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The objective of this study was to evaluate the effect of

feeding concentrate and fermented feed (Sorinfer) on the production
performance of Ongole Grade cattle (PO) heifer during the
recondition period. A total of 12 heads of PO heifers with an
average age of 1-2 years (I 0-I1) and initial body weight of
177.17±20.35 kg were used for the study. PO heifer was allotted
into three groups according to a combination of concentrate and
Sorinfer with a ratio of 70:30 (R1), 50:50 (R2), and 30:70 (R3).
Parameters observed were ambient temperature and humidity,
Temperature Humidity Index (THI), as feed consumption, dry
matter intake (DMI), crude protein (CP) intake, total digestible
nutrient (TDN) intake, conversion of as feed, dry matter conversion,
average daily gain (ADG), final live weight, and feed cost over gain

International Symposium on Innovative Approaches to Livestock Industry 2025 | 49

 (FCG). The data were analyzed by analysis of covariance
(ANCOVA), and differences between treatments were tested using
the least square means (LS means) method. The results showed that
as-fed consumption of R3 was significantly higher (P<0.05)
compared to the R1 and R2 treatments. However, DMI, CP, and
TDN intake of R1 was significantly lower (P<0.05) compared to R2
and R3.

Keywords: performance production, PO heifer, recondition,

Sorinfer

50 | International Symposium on Innovative Approaches to Livestock Industry 2025

Eco friendly animal feed resources and nutrition (OP-3)
Moderator: Dr. Nisa Nurmilati Barkah
Ballroom IICC 1

Time Abs No. Title Author

15.30-15.45 OP-3-1 Fatty Acid Characteristics of E. Sulistyowati,
Concentrate Containing Fermented I.K.G. Wiryawan, I.
Arenga pinnata on Milk of Dairy Cows Badarina, S.H.
Naibaho, Apreza,
W.A. Pratama, C.
Cahyadi, M.
Romdhoni and R. J.
Waspodo
15.45-16.00 OP-3-2 Impact of Encapsulated Cyanide- S. Suharti, A.
Degrading Bacteria on In Vitro Mislah, and I.
Fermentation and Microbial Population Wijayanti
in Bitter Cassava Leaf Meal-Based
Rations
16.00-16.15 OP-3-3 Digestive Organs, Total Leukocytes, Wahyuni, K.M.
and Spleen of Laying Quail with Ibadurrahman, R.
Maggot BSF in Feed Afnan, N. Ulupi,
I.I. Arief, and A.
Jayanegara
16.15-16.30 OP-3-4 In Vitro Evaluation of Carob (Ceratonia I. G. Permana, S.
siliqua L.) as An Energy Sources in Salehudin, D.
Dairy Ration Evvyernie, and A.
Rosmalia
16.30-16.45 OP-3-5 Supplementation of Brotowali Stem Muhammad A. A.
Meal (Tinospora Crispa L) in the Diet A. N, R. Mutia, and
on Performance, and Organ Immunity W. Hermana
of Hybrid Ducks
16.45-17.00 OP-3-6 Giblets, Malondialdehyde (MDA) Sumiati, R. Mutia,
Levels in Meat, and Carcass Quality of N. Septiandini, A.
Broiler Chickens Supplemented with A. Farhah, and R.
Vitamin E and Selenium in Feed Nadia
Containing Lemuru Fish Oil

International Symposium on Innovative Approaches to Livestock Industry 2025 | 51

Fatty Acid Characteristics of Concentrate Containing
Fermented Arenga pinnata on Milk of Dairy Cows

E. Sulistyowati1*, I. K. G. Wiryawan2, I. Badarina1, S. H.

Naibaho1, Apreza1, W. A. Pratama1, C. Cahyadi1, M. Romdhoni2
and R. J. Waspodo3
1
Animal Science Department., Faculty of Agriculture, University of
Bengkulu, Indonesia
2
Animal Production Department, Faculty of Animal Husbandry, IPB
University Bogor, Indonesia
3
Nutrition Laboratory, Faculty of Fishery, Graduate School, IPB
University Bogor, Indonesia
E-mail correspondence: [email protected]

Abstract. This study was aimed to evaluate the effect of diet with
concentrate containing fermented Arenga pinnata by-product on
fatty acid characteristics in concentrate, milk, and feces of dairy
cows. Pleurotus ostreatus was used to inoculate Arenga pinnata by-
product for five weeks until the full hypha was grown in the bag
logs. This biomass was then ready to be used as one of the
ingredients in the concentrate. The concentrates contained of four
ratios of fermented Arenga pinnata by-product to rice bran. The
ratios were CA0: diet with no fermented Arenga /35% rice bran in
concentrate; CA10: diet with 10% fermented Arenga/25% rice bran
in concentrate; CA15: diet with 15% fermented Arenga/20% rice
bran in concentrate; CA20: diet with 20% fermented Arenga/15%
rice bran in concentrate. Other ingredients were ground corn,
soybean meal, palm oil, mineral mix, Curcuma xanthorrhiza, yeast,
NaCl, CaCO3, and TSP. Those were all in the same amount in the
total of 65% in the concentrates. This arrangement used Latin
Square design with four lactating dairy cows. The results showed
that all characters of fatty acid (MCFA, LCFA, MUFA, PUFA, P/S)
were decreasing with increasing fermented Arenga pinnata- by
product in concentrates; while, saturated fatty acid increased. In
milk, there were improvement in LCFA, MCFA, MUFA, and P/S
supported with decreasing saturated fatty acid. This trend was also
found in the fatty acid in feces. With the concerns on some fatty

52 | International Symposium on Innovative Approaches to Livestock Industry 2025

 acids characters which found improvement in milk, it is concluded
that concentrate containing 20% fermented Arenga pinnata- by
product in combination with 15% rice bran is considered reasonable
fed for lactating dairy cow.

Keywords: acids, arenga, characteristics, concentrate, fatty,

fermented.

Impact of Encapsulated Cyanide-Degrading Bacteria on In

Vitro Fermentation and Microbial Population in Bitter
Cassava Leaf Meal-Based Rations

S. Suharti1*, A. Mislah1, and I. Wijayanti1

1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Bitter cassava leaves contain high protein around 21%,

but also have toxic compounds, namely cyanide acid (HCN), which
are harmful to livestock. Cyanide-degrading bacteria (CDB) that we
already isolated can detoxify HCN. Furthermore, the CDB isolate
needs to be capsulated for easy storage and distribution. This study
aimed to observe the effect of encapsulated cyanide-degrading
bacteria (ECDB) supplementation on in vitro fermentation with
bitter cassava leaf meal-based ration. The treatments were P0 =
control ration (Napier grass: bitter cassava leaf
meal:concentrate=30:30:40), P1 = P0 + ECDB (0-day storage), P2 =
P0 + ECDB (7-day storage), P3 = P0 + ECDB (21-day storage), P4
= P0 + ECDB (28-day storage). Variables observed include pH
value, NH3 concentration, dry matter digestibility (DMD) and
organic matter digestibility (OMD), volatile fatty acid (VFA)
concentration and its proportionality, methane estimation, and
rumen microbial population. The results showed that ECBD with
different shelf lives increased (p<0.05) rumen pH, total bacterial
population, and NH3 concentrations. Whereas DMD, OMD, VFA,

International Symposium on Innovative Approaches to Livestock Industry 2025 | 53

 and protozoa populations were similar among treatments. In
conclusion, the addition of encapsulated cyanide-degrading acid
bacteria stored for up to 28 days at room temperature has a
beneficial effect on the bacteria population and feed protein
degradation.

Keywords: bacteria population, bitter cassava leaf, degrading

cyanide bacteria, encapsulation, in vitro fermentation.

54 | International Symposium on Innovative Approaches to Livestock Industry 2025

Digestive Organs, Total Leukocytes, and Spleen of Laying
Quail with Maggot BSF in Feed

Wahyuni1*, K. M. Ibadurrahman1, R. Afnan1, N. Ulupi1, I. I.

Arief1, and A. Jayanegara2
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Feed is the main factor in the success of quail egg

production. However, feed prices often increase. Maggots can be a
solution for a feed protein source to replace MBM. This research
aims to analyze the size of the digestive organs, spleen, and total of
leukocytes of laying quail feed with maggot BSF. This research was
carried out at Arkan Quail Farm from November 2023 to March
2024. The parameters observed were the relative weight and relative
length of digestive organs, the relative weight of immune organs,
and total leukocytes. Data were analyzed using a t-test with 2
treatments (R0: feed without BSF maggots and R1: feed with BSF).
Each treatment was given five repetitions using ten quail during the
laying period (40 weeks old). The research showed that giving
maggots to feed did not significantly affect the weight percentage
and relative length of the digestive organs, relative weight of
Immune organs, and total leukocytes of the laying quail. It showed
that giving BSF and MBM maggots in feed is equally good.
Therefore, it can be concluded that giving BSF maggots with a
percentage of 12% in the feed does not negatively impact the
digestive and immune organs.

Keywords: BSF maggot, digestive organ, immunity organ, quail.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 55

In Vitro Evaluation of Carob (Ceratonia siliqua L.) as An
Energy Sources in Dairy Ration

I. G. Permana1*, S. Salehudin1, D. Evvyernie1, and A. Rosmalia1

1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Carob (Ceratonia siliqua L.) is a promising energy source

for dairy rations due to its high carbohydrate content (48–89%).
However, its tannin content may have negative impacts, requiring
careful adjustment in feed formulation. Research on carob in
tropical dairy rations is limited. This study evaluates carob's effects
on rumen fermentation and digestibility in dairy cattle using a
randomized block design (RBD) with five treatments and four
groups for rumen fluid collection. Treatments included R1 (6%
cassava waste), R2 (6% molasses), R3 (3% carob powder + 3%
cassava waste), R4 (3% carob powder + 3% molasses), and R5 (6%
carob powder). The parameters measured were rumen pH, ammonia
(NH₃) levels, volatile fatty acids (VFA), rumen bacterial and
protozoan populations, as well as dry matter digestibility (DMD)
and organic matter digestibility (OMD). Data were analyzed using
ANOVA followed by Duncan’s test. Results showed that carob
inclusion up to 6% (R5) significantly increased total VFA and
bacterial populations. No significant effects were observed on
rumen pH, NH3 levels, protozoa populations, DMD, and OMD.
Carob inclusion produced similar results to molasses and cassava
waste across most parameters, except for VFA levels and bacteria,
suggesting that carob can be used as a substitute for common energy
sources in dairy cattle rations.

Keywords: carob, carbohydrate, fermentability, energy sources,

digestibility.

56 | International Symposium on Innovative Approaches to Livestock Industry 2025

Supplementation of Brotowali Stem Meal (Tinospora Crispa L)
in the Diet on Performance and Organ Immunity of Hybrid
Ducks

A. A. A. N. Muhammad1*, R. Mutia1, and W. Hermana1

1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Efforts to overcome pathogenic microbial infections in

poultry farms still depend on antibiotics. However, using the
Antibiotic Growth Promoter (AGP) in poultry feed ingredients has
been prohibited, so herbal feed additives such as brotowali are
needed. This research aimed to evaluate the effect of feeding
brotowali stem meal on the performance and organ immunity of
hybrid ducks. The livestock used were 200 hybrid ducks, kept from
11 to 46 days of age. Ducks were fed basal diet as control (Tl), basal
diet supplemented with 0.5%, 1%, and 1.5% brotowali stem meal
(T2, T3, T4). The observed organs are the thymus, fabricius
exchange, and spleen. The research was carried out in Bogor, West
Java, while the brotowali phytochemical analysis was carried out at
the Biofarmaka Tropika IPB. All data collected within the present
study were analyzed using analysis of variance, and if there were
significant differences, further tests needed to be performed using
Duncan’s multiple range test by SPSS version 25. The results
showed brotowali stem meal did not significantly (p>0.05) influence
the immunity of hybrid ducks. Brotowali stem meal significantly
(p<0.05) improved the performances of the hybrid ducks, as
indicated by higher body weight gain and decreased feed conversion
ratio. In conclusion, brotowali can improve the performance of
hybrid ducks, although it does not affect immunity.

Keywords: brotowali stem meal, hybrid ducks, immunity organ,

performances.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 57

58 | International Symposium on Innovative Approaches to Livestock Industry 2025
Giblets, Malondialdehyde (MDA) Levels in Meat, and
Carcass Quality of Broiler Chickens Supplemented with
Vitamin E And Selenium in Feed Containing Lemuru Fish
Oil

Sumiati1*, R. Mutia1, N. Septiandini1, A. A. Farhah1,

and R. Nadia1
1
Department Nutrition and Feed Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB
Dramaga, Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The aimed of this study was to evaluate the effects

of supplementation of vitamin E and selenium on giblets,
malondialdehyde (MDA) in the meat, and carcass quality of
broiler chickens. A completely randomized factorial design
(CRFD) 2x3 with 3 replications and each used 1 bird, consisting
of 2 levels of vitamin E supplementation (0 ppm, 200 ppm) and
3 levels of selenium supplementation (0 ppm, 0.3 ppm, and 0.6
ppm) was used in this experiment. Data were analyzed using
analysis of variance (ANOVA). The results showed that there
was interaction between supplementation of 200 ppm vitamin E
and 0.6 ppm selenium in decreasing (p<0.05) the weight
percentage of kidney and abdominal fat. Supplementation of
200 ppm vitamin E highly significantly decreased (p<0.01) and
supplementation of 0.6 ppm selenium significantly decreased
(p<0.05) MDA contained in the meat. Supplementation of
vitamin E or selenium did not affect the carcass quality. It is
concluded that supplementation of 200 ppm vitamin E or 0.6
ppm selenium effective as an antioxidant without bothering of
carcass production and digestive tract of broiler
chickens. Combination of supplementation of 200 ppm vitamin
E and 0.6 ppm selenium effective decreased the fat abdominal.

Keywords: antioxidant, broiler chicken, lemuru fish oil,

selenium, vitamin E.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 59

60 | International Symposium on Innovative Approaches to Livestock Industry 2025
Frontier animal product technology (OP-4)
Moderator: Dr. M. Sriduresta Soenarno
Room 3

Time Abs No. Title Author

15.30-15.45 OP-4-1 Physicochemical Quality and Sensory M. I. Sylvani, H.
Evaluation of Reduced Fat Mayonnaise Evanuarini, P. P.
Prepare Using Aloe vera powder as Rahayu
Stabilizer
15.45-16.00 OP-4-2 Grilled Beef Characteristics Marinated T. Suryati, A. M.
Using Sweet Based Seasoning Added Ramadhani, B. U.
Etlingera elatior Flower for One or Alghifary, A.
Twelve Hours Apriantini, and M.
Arifin
16.00-16.15 OP-4-3 Utilization of β-Galactosidase (Lactase) M. Arifin, A.
Enzyme to Produce Lactose-Free Cow's Detiara, E. Taufik,
Milk and Its Derivative Product M. S. Soenarno, Z.
Wulandari, T.
Suryati, C.
Budiman, A.
Apriantini,
Salundik, D.
Murtini, R.R.S.
Wihansah
16.15-16.30 OP-4-4 Characteristics of Pasteurized Liquid S. F. Putri, H.
Eggs Yolk with Salt Addition to Evanuarini, P. P.
Improve the Stability Rahayu
16.30-16.45 OP-4-5 Utilization of Roselle Greek Yogurt as I.I. Arief, Z.
a Filling in Pastry Products and Its Wulandari, M.
Impact on Physicochemical and Assalamah, R.J.
Microbiological Quality During Furkon, D. Murtini,
Storage M Arifin and M.S.
Soenarno
16.45-17.00 OP-4-6 Whole genome sequence of meat- C. Budiman, I.I.
derived Lactobacillus paracasei IIA and Arief, K.F.
recombinant production of its protease Rodrigues, F.
Opok, R. Razali, O.
Mega, C. Sumantri,
and V. Kumar

International Symposium on Innovative Approaches to Livestock Industry 2025 | 61

Physicochemical Quality and Sensory Evaluation of Reduced
Fat Mayonnaise Prepare Using Aloe vera Powder as Stabilizer

M. I. Sylvani1, H. Evanuarini2*, and P. P. Rahayu2

1
Student of Animal Product Technology, Faculty of Animal Science,
Universitas Brawijaya, Veteran Street, Malang 65145, Indonesia
2
Lecturer of Animal Product Technology, Faculty of Animal
Science, Universitas Brawijaya, Veteran Street, Malang 65145,
Indonesia

E-mail correspondence: [email protected]

Abstract. Full fat mayonnaise raises consumer concerns about

degenerative diseases such as high cholesterol and obesity, creating
a demand for reduced fat alternatives. Reduced fat mayonnaise,
however, often suffers from emulsion instability due to the lower oil
content. Adding natural stabilizers like Aloe vera powder can
improve emulsion stability and increase viscosity. This research
aims was to determine the optimal percentage of Aloe vera powder
to enhance the physicochemical and sensory qualities of reduced fat
mayonnaise. The materials used was reduced fat mayonnaise made
from canola oil, vinegar, yolk, sugar, salt, pepper, and mustard, with
2%, 4%, and 6% Aloe vera powder. The experimental laboratory
method used was a completely randomized design (CRD) with 4
treatments and 5 replications. Data analysis was conducted using
ANOVA, followed by Duncan’s Multiple Range Test (DMRT). The
results of this research showed that Aloe vera powder decreased
moisture content and pH, but increased acidity, viscosity, and
emulsion stability. The research concludes that addition of 6% Aloe
vera powder gave the best reduced fat mayonnaise based on
physicochemical quality and sensory evaluation.

Keywords: Aloe vera, emulsion, mayonnaise, stabilizer.

62 | International Symposium on Innovative Approaches to Livestock Industry 2025

Grilled Beef Characteristics Marinated Using Sweet Based
Seasoning Added Etlingera elatior Flower for One or Twelve
Hours

T. Suryati1*, A. M. Ramadhani1, B. U. Alghifary1, A. Apriantini1,

and M. Arifin1
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. This study aimed to analyze the characteristics of grilled

beef marinated using sweet based seasoning (SBS) added EEF for 1
or 12 hrs marinating duration. Beef short plates come from the same
breed and age cattle were used in this study. SBS used in this study
consisted of honey (37%), oyster sauce (23%), worcestershire sauce
(14%), pepper (3%), garlic (21%), and sesame oil (3%). Beef was
sliced with a thickness of 3.5 mm, and then treated without
marination (control), marination with SBS without EEF addition, or
marination with SBS plus 10% EEF ground. The marination process
was carried out for 1 or 12 hrs. Before analyses, beef was grilled for
5 mins with flipping every min. The use of SBS in marinating
process resulted in grilled beef with lower pH (p<0.05), higher
antioxidant activity (p<0.05), and better sensory characteristics
(except for tenderness attribute) than control. The use of EEF into
marinade seasoning and duration marinating (1 or 12 hrs) had no
significant effect for all variables. As a conclusion, marinating in
grilled beef processing with the SBS for 1 hr was more efficient than
12 hrs, and the use of SBS with or without EEF addition increased
antioxidant activity.

Keywords: antioxidant activity, Etlingera elatio, grilled beef,

marination, seasoning,

International Symposium on Innovative Approaches to Livestock Industry 2025 | 63

Utilization of β-Galactosidase (Lactase) Enzyme to Produce
Lactose-Free Cow's Milk And Its Derivative Product

M. Arifin1, A. Detiara1, E. Taufik1, M. S. Soenarno1, Z.

Wulandari1, T. Suryati1, C. Budiman1, A. Apriantini1, Salundik1,
D. Murtini1, and R.R.S. Wihansah2
1
Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia
2
Department of Animal Husbandry, Bogor Agricultural
Development Polytechnic, Bogor, 16119, Indonesia

E-mail correspondence: [email protected]

Abstract. People who are lactose intolerant avoid consuming milk

and dairy products. However, excluding milk and dairy products
from the daily diet can impact the intake of essential nutrients found
in milk. Lactose can be broken down into galactose and glucose
using β-galactosidase (Lactase) enzymes. Lactose-free milk, which
has a higher sweetness level than full-cream milk, is an ideal base
for making ice cream, a popular product known for its sweet taste
and unique texture. This study aims to analyze the physical,
chemical, and organoleptic properties of lactose-free ice cream. The
analysis includes assessments of physical characteristics, chemical
composition, and sensory qualities. A completely randomized
design (CRD) was used for the milk analysis, while a T-Test with
three repetitions was employed for the ice cream analysis. The
results indicated that lactose-free milk had no significant effect
(p>0.05) on viscosity, water activity (aw), pH, protein content, fat
content, solid non-fat content, or organoleptic qualities, but it had a
significant effect on lactose content (p<0.01). For lactose-free ice
cream, there was no significant effect (p>0.05) on overrun, pH
value, melting time, protein and fat content, as well as in the
hedonic tests for color, aroma, sweetness, and milk flavor. These
results show that lactose-free milk has characteristics similar to
whole milk.

64 | International Symposium on Innovative Approaches to Livestock Industry 2025

Keywords: β-galactosidase, cow milk, ice cream, lactose free.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 65

Characteristics of Pasteurized Liquid Eggs Yolk with Salt
Addition to Improve the Stability

S. F. Putri1, H. Evanuarini2*, and P. P. Rahayu2

1
Student of Animal Product Technology, Faculty of Animal Science,
Universitas Brawijaya, Veteran Street, Malang 65145, Indonesia
2
Lecturer of Animal Product Technology, Faculty of Animal
Science, Universitas Brawijaya, Veteran Street, Malang 65145,
Indonesia

E-mail correspondence: [email protected]

Abstract. Liquid eggs are a safe and practical form of processed

eggs, undergo homogenization and pasteurization, which can reduce
their functional properties. To maintain these properties, stabilizers
such as salt are used. Salt improves foaming stability and
expandability in liquid eggs. The objective of this study was
determining the best percentage of salt use in liquid eggs yolk in
terms of physicochemical characteristics. This study used fresh
chicken egg yolks and added different levels of salt (0.5%, 1%, and
1.5%) with a pasteurization process at 60°C for 2 minutes. The
method used in this study was a laboratory experiment with a
Completely Randomized Design (CRD) with 4 treatments and 5
replicates. The data analysis was performed using ANOVA,
followed by Duncan's Multiple Range Test (DMRT) if significant
differences were found. The results showed that the addition of salt
didn’t give significant effect on pH and color values. However, it
had a significant effect on yield, viscosity, foaming stability, and
moisture content. It can be concluded that the addition of salt to
liquid egg yolk has a significant effect on yield, viscosity, foaming
stability and moisture content, and improves the overall quality of
liquid egg yolk.

Keywords: liquid egg yolk, pasteurized, salt.

66 | International Symposium on Innovative Approaches to Livestock Industry 2025

Utilization of Roselle Greek Yogurt as a Filling in Pastry
Products and Its Impact on Physicochemical and
Microbiological Quality During Storage

I. I. Arief1*, Z. Wulandari1, M. Assalamah1, R. J. Furkon1, D.

Murtini1, M Arifin1, and M. S. Soenarno1
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Pastry is a type of dough used to make baked goods,

characterized by a flaky, crisp, or tender texture. Strudel, a specific
type of pastry, is rolled out and filled with various ingredients,
primarily fruits. This study investigates the physicochemical and
microbiological properties of strudel pastry filled with roselle Greek
yogurt during storage at room temperature. The design research used
was randomized complete factorial design with two treatments and
three replications. The treatments included different fillers;
condensed milk and roselle Greek yogurt; and storage durations of
0, 2, and 4 days at room temperature. Results showed that in strudel
with condensed milk, the moisture content is 27.86%, protein
content is 7.25%, and fat content is 25.6%. In contrast, for strudel
with yogurt filled, the moisture content is 24.46%, protein content is
8.68%, and fat content is 24.25%. After 2 days of storage, strudel
with yoghurt still meet microbiological quality standards according
to SNI, where the maximum allowable mold and yeast is 2 log
CFU/g. Strudel with yogurt filled contains lactic acid bacteria at
3.71 log CFU/g, mold and yeast at 1.96 CFU/g, and total microbes
at 4.48 log CFU/g. In comparison, strudel with condensed milk
contains lactic acid bacteria at 3.38 log CFU/g, mold and yeast at
2.58 log CFU/g, and total microbes at 4.61 log CFU/g.
Keywords: greek yogurt, pastry, roselle, storage.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 67

Whole Genome Sequence of Meat-derived Lactobacillus
paracasei IIA and Recombinant Production of Its Protease

C. Budiman1, I. I. Arief1, K. F. Rodrigues2, F. Opok2, R. Razali2,

O. Mega3, C. Sumantri1, and V. Kumar2
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Bogor 16680, Indonesia
2
Biotechnology Research Institute, Universiti Malaysia Sabah, Kota
Kinabalu, Sabah, Malaysia
3
Department of Animal Science, Faculty of Animal Science,
University of Jambi. Jambi 36361, Indonesia

E-mail correspondence: [email protected]

Abstract. Lactobacillus paracasei IIA-1A5, a lactic acid bacterium

isolated from Indonesian cattle, shows promise for probiotic use due
to its inhibitory effects on pathogenic bacteria. This study aims to
identify functional genes of this strain through complete genomic
analysis and to produce its industrial enzyme using recombinant
techniques. Using a Pacific Biosciences RSII Sequencer, the
genome was sequenced, revealing a 3,055,892 bp chromosome with
46.59% G+C content and three plasmids. The genome includes
3,161 genes, encoding 3,007 proteins and various RNA genes.
Unique features include genes for monosaccharide metabolism and a
toxin-antitoxin system, potentially aiding adaptation to lactose-free
environments like meat. Additionally, the genome revealed a lon-
like protease (Lon-Pro) gene, known for its versatile applications.
To produce this enzyme recombinantly, the gene was inserted into a
pET28a system, expressed in Escherichia coli BL21(DE3), and
purified using Ni-NTA affinity chromatography. The purified Lon-
Pro enzyme was shown to be catalytically active against casein,
skim milk, collagen, gelatin, sarcoplasmic, and myofibrillar
proteins. This suggested that this protease to reduce the use of
chemical treatment in leather processing. These findings provide
valuable insights into the genetic mechanisms enabling L. paracasei

68 | International Symposium on Innovative Approaches to Livestock Industry 2025

IIA-1A5 to thrive in such environments and suggest potential
functional genes for further applications.

Keywords: lactic acid bacteria, Lactobacillus, probiotic, protease,

recombinant protein, wholge genome sequence.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 69

70 | International Symposium on Innovative Approaches to Livestock Industry 2025
Animal welfare, health, and environment (OP-5)
Moderator: Dr. Reza Adiyoga
Room 4

Time Abs No. Title Author

15.30-15.45 OP-5-1 The Effect of Adding Penicillin on The Parsaoran Silalahi
Quality of Pig Semen
15.45-16.00 OP-5-2 Reducing Nitrogen Losses in Dairy W.A Zahra, M.
Farms: The Effects of Gutter Arifin, Salundik,
Modifications in a Tie Stall System S.D.Oktarina, I.
Rosmayanti and L.
Priskila
16.00-16.15 OP-5-3 Dynamics of the Physiological D. N. Humaira, L.
Response in Garut Sheep to Different Khotijah, R. I.
Fatty Acid Sources and Antioxidants Arifiantini, Herdis,
and A. Nurlatifah
16.15-16.30 OP-5-4 Screening the Wound Healing Potential A. Y. Prawira, R.
Compounds in Ethanolic and N-Hexane Fidriyanto, R. F.
Extract of Porcupine Quill Using Yuneldi, T. H.
Untargeted Metabolomic Analysis Handayani, R. L. R.
Approach Amalia
16.30-16.45 OP-5-5 Supplementation of lauric acid from Faza Baihaqi, D. A.
BSF oil as anti-diarrhea in growing Astuti, M. A.
Garut sheep Setiadi,
Purwantiningsih, A.
Tarigan
16.45-17.00 OP-5-6 Analysis of Factors Causing Death of Cyrilla, L., Sinaga,
Pigs Due to African Swine Flu S., Sipayung, G.F
Outbreak in Toba District, North
Sumatra Province

International Symposium on Innovative Approaches to Livestock Industry 2025 | 71

The Effect of Adding Penicillin on The Quality of Pig Semen

P. Silalahi1*, T. F. Sitorus1, A. C. Tarigan1, and P. Panjaitan1

1
Study Program Animal Husbandry, Faculty of Animal Husbandry,
University of HKBP Nommensen, Jl. Sutomo, Medan 20234,
Indonesia

E-mail correspondence: [email protected]

Abstract. One type of antibiotic that can be used in the semen

dilution process is penicillin. The penicillin can inhibit the growth
of gram-positive bacteria. This study aimed to determine the effect
of penicillin addition in the pig semen dilution process on the
quality of pig semen after 24 hours of dilution. The pig used in this
study was a male pig over 2 years old. The method used was a
Completely Randomized Design (CRD), consisting of 4 treatments
with 4 replications, namely P0 without penicillin in the diluent and
P1, P2 and P3 with 0.3, 0.6 and 0.9 gr/l penicillin in the diluent
respectively. The extender used in this study was the non-antibiotic
Beltsville Thawing Solution (BTS). The parameters observed were
Motility, Viability, Sperm Abnormalities and pH. The data was
analysed using Analysis of Variance (ANOVA) and continued with
HSD test when significant different was observed between the
treatments. The results showed that the addition of penicillin was
not significantly different (P>0.05) on pH and abnormalities, but
was significantly different (P<0.05) on motility, and very
significantly different (P<0.01) on viability of sperm. The addition
of 0.3 g/l penicillin was the best level in the pig semen dilution
process.

Keywords: penicillin, pig, semen dilution, semen quality.

72 | International Symposium on Innovative Approaches to Livestock Industry 2025

Reducing Nitrogen Losses in Dairy Farms: The Effects of
Gutter Modifications in a Tie Stall System

W.A Zahra1, M. Arifin1, Salundik1, S.D. Oktarina2,

I. Rosmayanti1, and L. Priskila1
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department of Statsitic, Faculty of Mathematics and Natural
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Manure from dairy cows, consisting of feces and urine,

presents a significant issue for smallholder dairy farms in Indonesia.
Managing feces is relatively easy as it can be collected and stored
for use as organic fertilizer. However, most urine is discharged and
lost to the environment. This study aims to analyze the potential
reduction of nitrogen (N) losses from urine by modifying the gutter
system.We modified the gutter by constructing the urine gutter
which separated with the feces gutter on a smallholder dairy farm in
Lembang, West Java, with five lactating cows and two dry cows.
The separated urine line was directed to temporary storage and
processed as liquid processed manure (POC). We calculated the
amount of urine excreted per cow over 24 hours to estimate the N
content in the urine. Our findings showed the average excreted urine
of 15 liters per day, totalling 125 liters per farm or 3770 liters per
month. By modifying the gutter system, 880 kg of urine has been
captured, which equals to 23.3% of the total urine per month. With a
0.5% nitrogen content in urine, the potential N loss was 18.8 kg, and
the avoided losses were 4.4 kg per month. Modifying the gutter can
thus reduce N losses from urine production.

Keywords: manure management, nitrogen losses, dairy cows.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 73

 Dynamics of The Physiological Response in Garut Sheep
to Different Fatty Acid Sources and Antioxidants

D. N. Humaira1*, L. Khotijah1, R. I. Arifiantini2, Herdis3,

and A. Nurlatifah4
1
Department Nutrition and Feed Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB
Dramaga, Bogor 16680, Indonesia
2
Department of Clinical, Reproduction, and Pathology, School
of Veterinary Medicine and Biomedical Sciences, IPB
University, Jl. Agatis Kampus IPB Dramaga, Bogor 16680,
Indonesia
3
Research Center for Animal Husbandry, National Research
and
Innovation Agency, Bogor 16915, Indonesia
4
Faculty of Animal Science, Gadjah Mada University, 555281

E-mail correspondence: [email protected]

Abstract. Indications of heat stress in sheep can be seen from

physiological responses influenced by climate and feed factors.
This study aimed to evaluate the effects of various unsaturated
fatty acid sources enriched with antioxidants on the
physiological responses of Garut sheep. Twenty Garut rams,
aged 12-14 months and weighing 33.1 ± 4.9 kg, were assigned
to four treatments in a 4 x 2 factorial design. The first factor
was feed (control, 4.2% canola oil, 4.2% sunflower seed oil,
and 4.2% lemuru fish oil, each oil supplemented with vitamin E
500 IU and selenium 0.5 ppm), and the second factor was
observation time (morning, noon, and afternoon). Physiological
responses were measured, including rectal temperature, heart
rate, and respiratory rate. The results showed no significant
interaction between feed treatment and observation time
(P>0.05). Feed treatment and observation time significantly
affected (P<0.05) the physiological response. The addition of
4.2% lemuru oil + antioxidants significantly lowered (P<0.05)
respiratory rate within the mild stress range and heart rate within
the normal range by the 3rd week of treatment. It can be
concluded that sheep given 4.2% lemuru oil + antioxidants had
better physiological responses, which correlated with lower
levels of heat stress.

Keywords: antioxidants, fatty acids, Garut rams,

physiological

74 | International Symposium on Innovative Approaches to Livestock Industry 2025

Screening The Wound Healing Potential Compounds in
Ethanolic and N-Hexane Extract of Porcupine Quill Using
Untargeted Metabolomic Analysis Approach

A. Y. Prawira1*, R. Fidriyanto1, R. F. Yuneldi1, T. H.

Handayani1, and R L R Amalia1
1
Research Center for Applied Zoology, Research Organization for
Life Science and Environment, National Research and Innovation
Agency, Cibinong, Indonesia. Jl. Raya Bogor Km 46, Cibinong,
Bogor 16911, West Java, Indonesia

E-mail correspondence: [email protected]

Abstract. Porcupine quills, besides being used in traditional

communities in Indonesia, its ethanol and n-hexane extracts have
been demonstrated to facilitate wound healing, reduce pain, and
minimize scarring in rats. However, there is currently no detailed
information available regarding the specific compounds in
porcupine quill extracts that have the potential to improve wound
healing. This study aimed to screen the compounds in ethanol and n-
hexane extracts of porcupine quills to identify the wound-healing
potential compounds. This study utilized 90 g of naturally detached
porcupine quills, extracted and identified using untargeted
metabolomic analysis in LC-HRMS and bioinformatic analysis. The
findings indicated the ethanol extract exhibited higher relative
abundance than the n-hexane extract, with fatty acyls identified as
predominant compound (38%). A total of 33 compounds were
identified in both extracts, while the specific compounds in ethanol
extract and n-hexane extract were 44 and 36 compounds. Four
compounds exhibited the highest abundance in both extracts and
were involved in improving wound healing: α-Eleostearic acid, 9-
Oxo-10(E),12(E)-octadecadienoic acid, Stearamide, and Palmitoyl
ethanolamide. The other compounds were D-sphingosine,

International Symposium on Innovative Approaches to Livestock Industry 2025 | 75

 cholecalciferol, palmitoleic acid, 11(Z),14(Z),17(Z)-eicosatrienoic
acid, and arachidonic acid. The study showed that ethanol and n-
hexane extract of porcupine quill contain compounds that may
improve wound healing.

Keywords: compound, extract, fatty acyls, porcupine quill, wound

healing.

76 | International Symposium on Innovative Approaches to Livestock Industry 2025

Supplementation of Lauric Acid from BSF Oil as Anti-
diarrhea in Growing Garut Sheep

F. Baihaqi1*, D. A. Astuti2, M. A. Setiadi3, Purwantiningsih4, and

A. Tarigan5
1
Graduate School of Nutrition and Feed Technology, IPB University
Department of Animal Nutrition
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Bogor 16680, Indonesia
3
Department of Veterinary Clinic, Reproduction and Pathology,
Faculty of Veterinary Medicine, IPB University, Bogor 16680,
Indonesia
4
Department of Chemistry, Faculty of Mathematics and Natural
Sciences, IPB University, Bogor 16680, Indonesia
5
Research Center for Animal Husbandry, National Research and
Innovation Agence (BRIN), Jl. Jakarta-Bogor Km.46 Cibinong
16911, Bogor, Indonesia

E-mail correspondence: [email protected]

Abstract. Decreased immunity in Garut sheep often reduces

productivity and presents a complex challenge. Lauric acid, an
antimicrobial agent, may enhance immunity in these sheep. This
study aimed to evaluate the effects of lauric acid from black soldier
fly (BSF) oil on feed intake, lauric acid intake, average daily gain
(ADG), and anti-diarrheal properties in vivo. A complete block
design (CBD) with four treatments (was used: P0: (Ration + 2%
BSF Oil), P1: (Ration + 2% Coated Lauric Acid), P2: (Ration + 2%
Uncoated Lauric Acid), and P3: (Ration + 2% Palm Oil), each with
four replicates. Data were analyzed using variance analysis
(ANOVA) followed by Duncan's Test. Results showed that Coated
Lauric Acid, Uncoated Lauric Acid, and Palm Oil treatments
significantly (P<0.05) reduced the number of E. coli in feces
compared to BSF oil, while feed intake, lauric acid intake, and ADG
were not significantly affected (P>0.05). Supplementation with 2%
Coated Lauric Acid, Uncoated Lauric Acid, or Palm Oil effectively

International Symposium on Innovative Approaches to Livestock Industry 2025 | 77

 reduced E. coli in the feces of Garut sheep without negatively
impacting feed intake, lauric acid intake, or ADG.

Keywords: antimicrobial, bsf, coating, garut sheep, lauric acid.

78 | International Symposium on Innovative Approaches to Livestock Industry 2025

Analysis of Factors Causing Death of Pigs due to African
Swine Flu Outbreak in Toba District, North Sumatra
Province

L. Cyrilla1*, S. Sinaga2, and G. F. Sipayung3

1
Faculty of Animal Science IPB University, Jl, Agatis Kampus IPB
Dramaga Bogor
2
Faculty of Animal Husbandry Pajajaran University, Jalan Raya
Bandung Sumedang KM 21, Jatinangor
3
Dinas Pertanian Kabupaten Toba Jalan Pertanian No . 1 Hutabulu-
Mejan Balige

E-mail correspondence: [email protected]

Abstract. This study aims to analyze the factors causing livestock

death in pig farms due to African Swine Flu (ASF) outbreak in Toba
District, North Sumatra Province. The research was conducted at a
community pig farm in Toba District, North Sumatra Province. Data
collection was carried out from May to July 2022. Data collection
was carried out by interviewing farmers, as well as conducting
direct observations in the pens of pig farms in Toba Regency. Sixty
five farmers were selected as respondents in this study. Factors
causing the death of pigs were analyzed using logistic regression.
The dependent variable used is pigs infected with ASF (dead pigs)
with a value of 1, and a value of 0 if there are no pigs infected with
ASF. Variables that have a significant influence on the number of
deaths of pigs in smallholder farms in Toba Regency are: farmer's
education level, main occupation of the farmer, location of pens,
distance of pens from other farmers' pens, housing systems, sanitize
goods that will enter the pen, use of disinfectants, origin of male or
boar, type of feed given, feed processing before being given to pigs,
and carrying out fumigation.

Keywords: African Swine Flu, outbreaks, pig death’ factors, small

pig farms.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 79

80 | International Symposium on Innovative Approaches to Livestock Industry 2025
International Symposium on Innovative Approaches to Livestock Industry 2025 | 81
 ORAL PRESENTATION (SECOND DAY)

Sustainable animal genetic resources & Frontier Animal Product

Technology (OP-6)
Moderator : Dr. Kasita Listyarini
Room 1

Time Abs No. Title Author

12.30-12.45 OP-6-1 No Association between SNP in FATP1 A. Furqon, N.
Gene and Carcass Traits in Kampung Ulupi, T. Suryati,
Chicken A. Gunawan, and
C. Sumantri
12.45-13.00 OP-6-2 ND1 Gene Analysis in Beef Cattle for N. Mulyati, Y. G.
Sustainable Genetic Resource M. Bonawati, R.
Management R. Noor, M. F.
Ulum, and Jakaria
13.00-13.15 OP-6-3 Prolactin Gene in Duck Based on Latifah, Y.
Genbank Data Sequences: A Preliminary Sopian, U. F.
Study Handayani, L.
Anggraini, and Y.
L Dewi
13.15-13.30 OP-6-4 The Influence of IBA, NAA, IAA Types L. Liman, R.
of Plant Growth Regulators and Their Rohana, F. T.
Concentrations on The Growth of Farda , E.
Pakchong Grass Cuttings (Pennisetum Erwanto, M
purpureum cv. Thailand) Muhtarudin , and
V. Waniatie
13.30-13.45 OP-6-5 Antioxidant and Antibacterial Potential T.M. Alfiansyah,
of Pepsin-Hydrolyzed Bioactive Peptides Z. Wulandari, and
from IPB D1 Chicken Egg White C. Budiman
13.45-14.00 OP-6-6 Protein Profiles of Buffalo Milk Dangke A.Mutmainna,
as Traditional Product of Enrekang, I.Isnafia Arief, C.
South Sulawesi Budiman, Z.
Abidin and
Z.Wulandari
14.00-14.15 OP-6-7 Growth Performance of IPB-D2 with M. A. Rizqi, C.
IPB-D3 and Reciprocal Chicken Crosses Sumantri, and S.
Darwati
14.15-14.30 OP-6-8 Genetic and acoustic characteristics of M. Ulfah, S.
the pelung long-crowing cock for Rochana, A.
competition Farajallah, B.
Masy’ud

82 | International Symposium on Innovative Approaches to Livestock Industry 2025

No Association between SNP in FATP1 Gene and Carcass
Traits in Kampung Chicken

A. Furqon1*, N. Ulupi2, T. Suryati2, A. Gunawan2, and C.

Sumantri2
1
Research Center for Applied Zoology, National Research and
Innovation Agency, Bogor, Indonesia
2
Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Bogor, Indonesia

E-mail correspondence: [email protected]

Abstract. This study aimed to identify the genetic diversity of the

FATP1 gene associated with the carcass composition in Kampung
chicken. A total of 56 Kampung chickens aged 16 weeks were used
in this study, consisting of 26 male chickens and 30 female
chickens. DNA was amplified using Polymerase Chain Reaction
(PCR). Furthermore, the PCR products were incubated at 37 oC using
the AciI enzyme. Data of carcass compositions were collected
consisting of carcass weight (CW), breast weight (BW), thigh
weight (TW), drumstick weight (DW), wing weight (WW), and
back weight (BcW). We found three genotypes (AA, GG, and GA)
and two alleles (G and A). The AG genotype and A allele were the
most frequent in all populations of Kampung chicken. There was no
effect of SNP in the FATP1 gene on carcass composition in this
study. In descriptive terms, the AA genotype exhibited the highest
values in CW, TW, DW, and BcW. Conversely, the AG genotype
demonstrated the highest values in BW and WW. In conclusion, this
SNP has the potential to be developed as a molecular marker to
assist in breeding programs for Indonesian native chickens.

Keywords: FATP1, genetic diversity, kampung chicken, PCR-

RFLP, polymorphism.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 83

ND1 Gene Analysis in Beef Cattle for Sustainable Genetic
Resource Management

N. Mulyati1, Y. G. M. Bonawati1, R. R. Noor1, M. F. Ulum2, and

Jakaria1*
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Veterinary Medicine and Biomedicine College, IPB University, Jl.
Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The mitochondrial ND1 gene encodes the NADH-

ubiquinone oxidoreductase chain and can be considered a candidate
gene associated with adaptation to high-altitude environments. This
study aimed to identify sequence variations in the ND1 gene and to
assess the genetic relationships among Bali, PO, and Wagyu-Bali
crossbred cattle. Twelve samples were analyzed, consisting of 2
Bali, 5 PO, and 5 Wagyu-Bali crossbred cattle. Identification of
ND1 gene SNPs and phylogenetic analysis were performed using
the Neighbor-Joining method with 1,000 bootstrap replicates in the
MEGA7 software. Genetic diversity of the ND1 gene in beef cattle
revealed three SNPs: SNP c.459G/A, c.691T/A, and c.693T/C.
Therefore, the SNPs identified need further study about heat
resistance adaptation, particularly in Bali cattle and other breeds.
However, the phylogenetic tree analysis based on the ND1 gene did
not show clear distinctions among the cattle breeds studied.

Keywords: beef cattle, ND1 gene, phylogenetic tree, SNP, heat

tolerance.

84 | International Symposium on Innovative Approaches to Livestock Industry 2025

Prolactin Gene in Duck Based on Genbank Data Sequences: A
Preliminary Study

Latifah1, Y. Sopian2, U. F. Handayani1, L. Anggraini1, and Y. L

Dewi1
1
Research Center for Animal Husbandy, National Research and
Innovation Agency (BRIN), Cibinong 16911, Indonesia
2
Doctoral Program in Innovative Tropical Agriculture, School of
Industrial Education and Technology, King Mongkut’s Institute of
Technology Ladkrabang, Bangkok 10520, Thailand

E-mail correspondence: [email protected]

Abstract. The Prolactin gene is a candidate gene for egg production

in poultry. This study aimed to perform genetics analysis, including
single nucleotide polymorphisms (SNPs) identification, amino acid
change analysis, and restriction enzyme mapping. A preliminary
study on the prolactin gene was carried out in 8 GenBank sequences
of ducks. Polymorphism was screened in 8 prolactin gene sequences
of duck (AB158611.1; JQ677091.2; GU984377.1; DQ660983.1;
DQ345782.1; LC565023.1; NM001310372; DQ345783.1) using
alignment with Bioedit ver. 7.2. g. The results showed that 87 SNPs
were detected: 10 SNPs in the 5’UTR region, 3 SNPs in exon 1, 26
SNPs in intron 1, 4 SNPs in exon 2, 18 SNPs in intron 2, 2 SNPs in
exon 3, 8 SNPs in intron 3, 1 SNP in exon 4, 9 SNPs in intron 4; 5
SNPs in exon 5 and 2 SNPs in 3’UTR. There were 9 SNPs in the
exon region that changed amino acids. The main result of restriction
enzyme mapping discovered seven enzymes (AluI, BstKTI, DpnI,
MboI, AciI, FatI, NlaIII) which recognized 6 SNPs regions based on
restriction mapping using the Bioedit program. The results of this
study are further evidence for the role of Prolactin gene as a
candidate gene in the duck.

Keywords: duck, enzyme, preliminary study, genbank, prolactin

gene, SNP.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 85

The Influence of IBA, NAA, IAA Types of Plant Growth
Regulators and Their Concentrations on The Growth of
Pakchong Grass Cuttings (Pennisetum purpureum cv.
Thailand)

L. Liman1, R. Rohana1, F. T. Farda1*, E. Erwanto1, M.

Muhtarudin1, and V. Waniatie2
1
Program study of Nutrition and Feed Technology, Department of
Animal Husbandry, Faculty of Agriculture Lampung University, Jl.
Prof. Dr. Soemantri Brojonegoro No.1 Gedung Meneng, Bandar
Lampung 35145
2
Program study of Animal Husbandry, Department of Animal
Husbandry, Faculty of Agriculture Lampung University, Jl. Prof.
Dr. Soemantri Brojonegoro No.1 Gedung Meneng, Bandar
Lampung 35145

E-mail correspondence: [email protected]

Abstract. This research aims to determine the influence of the type

and concentration of external plant growth regulators (PGRs) on
growth of Pakchong grass. A factorial Completely Randomized
Design 3×3 with 3 replicates was employed in this study. The
experiment consists of two factors. The first factor was type of
PGRs consisting of P1: Indole acetic acid (IBA), P2:Naphthalene
acetic acid (NAA), and P3: Indole acetid acid (IAA), and the second
was the concentration of PGRs consisting of K1: 300ppm, K2:
400ppm, K3: 500ppm. The experimental plants were immersed in a
PGR solution according to the treatment. The experimental plants
were planted on plots with 0.5 x 2 meters dimension. The
parameters including percentage of growth, tiller number, leaves
number, plant weight. The data were analyzed using Analysis of
Variance (ANOVA) and further analyzed using Least Significant
Difference (LSD) test. The results showed that there was an
interaction effect of both factors (p<0.05) on plant height and plant
weight, but no significant effect (p>0.05) on the tiller number,
leaves number, and percentage of growth. Application of IAA at 500
ppm resulted in the best growth performance.

86 | International Symposium on Innovative Approaches to Livestock Industry 2025

Keywords: growth of Pennisetum purpureum cv. Thailand,
Pennisetum purpureum cv. Thailand, plant growth regulator.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 87

Antioxidant and Antibacterial Potential of Pepsin-Hydrolyzed
Bioactive Peptides from IPB D1 Chicken Egg White

T. M. Alfiansyah1, Z. Wulandari1*, and C. Budiman1

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Research on egg protein-derived bioactive peptides has

progressed during the past decades. Egg whites are known to have
bioactive activities such as antioxidants and antibacterials located in
the peptides of their proteins. IPB-D1 chicken is a new chicken
strain with genetic advantages including disease resistance,
adaptability, better growth performance than local chickens, and
high-quality egg production. IPB-D1 chicken eggs are expected to
be a new source of bioactive peptide-producing proteins. This study
aims to analyze the antioxidant and antibacterial activity of IPB-D1
chicken egg white bioactive peptides through enzymatic hydrolysis
with pepsin. In this study, enzymatic hydrolysis was conducted
using different incubation times (0, 6, and 12 hours) and varying
concentrations of pepsin enzyme (0, 50, and 100 units). The degree
of hydrolysis (DH) was measured to determine the efficiency of the
hydrolysis process. Among the treatments, the hydrolysis filtrate
with 100 units of pepsin enzyme and 12 hours of incubation showed
the best results, achieving a DH of 14.20%. The egg white filtrate
showed DPPH inhibitory activity of 14.97% and an antioxidant
capacity of 86.78 mg EVC 100 g-1. This inhibitory activity was
higher compared to the non- hydrolyzed egg white. Antibacterial
testing using the disc method showed inhibitory activity against both
Gram-positive and Gram- negative bacteria. The hydrolyzed egg
white filtrate exhibited better antibacterial activity than the non-
hydrolyzed egg white, with stronger inhibition observed against
Gram-positive bacteria.

Keywords: antibacterial, antioxidant, bioactive peptides, IPB-D1

chicken egg, pepsin.

88 | International Symposium on Innovative Approaches to Livestock Industry 2025

International Symposium on Innovative Approaches to Livestock Industry 2025 | 89
Protein Profiles of Buffalo Milk Dangke as Traditional
Product of Enrekang, South Sulawesi

A. Mutmainna1,3*, I. Isnafia Arief2, C. Budiman1, Z. Abidin2 and

Z.Wulandari1
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department Mathematics and Natural Science, Bogor Agricultural
University, Jl. Agatis Kampus IPB Darmaga, Bogor, West of Java,
16680, Indonesia
3
Department of Animal Science, Faculty of Science and
Technology, Universitas Islam Negeri Alauddin Makassar,
Indonesia

E-mail correspondence: [email protected]

Abstract. Dangke is a processed product of buffalo milk,

originating from South Sulawesi. It is made through a fermentation
process which facilitates coagulation responsible for its texture with
addition of some ingredients, which make it popular as a soft
cheese. In addition, lactic acid bacteria in raw buffalo milk are able
to hydrolyse casein into peptides, a smaller form of protein
fragments. This work aimed to investigate protein profiles of
dangke collected from three locations in Enrekang, South Sulawesi.
Molecular size and purity of the fractions were determined using
SDS-PAGE experiment. The amount of purified protein byof
dangke was quantified using Lowry protocols. As a result, P2
showed the highest protein content of 19.36%. The dangke samples
had different protein concentration as follows: P1 (12.29 mg/ml), P2
(14.54 mg/mL), and P3 (13.07 mg/mL). Based on SDS-Page
analysis, the highest protein band for dangke was detected in P2
with 26.5 kDa which was much lower than that for buffalo milk
with P2 71.9 kDa. In summary, the differences in protein profiles,
protein concentration, and molecular weight between dangke

90 | International Symposium on Innovative Approaches to Livestock Industry 2025

samples from different sites revealed proteolysis activities by
protease.

Keywords: buffalo milk, dangke, molecular weight, protein content,

protein concentration.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 91

Growth Performance of IPB-D2 with IPB D-3 and Reciprocal
Chicken Crosses

M. A. Rizqi11*, C. Sumantri2, and S. Darwati2

1
Post Graduate Student, Animal and Production Technology, Faculty
of Animal Science, IPB University, Indonesia
2
Department of Animal Production Technology, Faculty of
Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The potential of local chickens to be developed into

superior seeds will be a national asset. Efforts to increase the
productivity of local chickens are through crossbreeding. IPB-D1
chicken is a superior local chicken breeding result. The
development of IPB-D1 chickens produced prospective strains of
IPB-D2 and IPB- D3 chickens. IPB-D2 chicken is a candidate
female line with strong endurance traits, while IPB-D3 chicken is a
candidate line with fast growth traits. Crossing IPB-D2 chickens
with IPB-D3 and its reciprocals is expected to produce offspring
like their parents. This study aims to determine the growth
performance of IPB-D2 chicken crosses with IPB-D3 and their
reciprocals. The study used 100 DOC D2D3 and 100 DOC D3D2
raised in the Field Laboratory of Genetics and Animal Breeding.
Data were collected every 1 week including body weight, body
weight gain, feed consumption and feed conversion. Data were
analyzed descriptively and T-test to determine the average
difference in crossing results. The results showed that there was no
difference between the two crosses. The result of crossing IPB-D2
chicken with IPB-D3 and its reciprocal has the potential to be
developed as a superior local broiler.

Keywords: crosses, local chicken, performane, reciprocals.

92 | International Symposium on Innovative Approaches to Livestock Industry 2025

Genetic and Acoustic Characteristics of The Pelung Long-
Crowing Cock for Competition

M. Ulfah1*, S. Rochana1, A. Farajallah2, and B. Masy’ud3

1
Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Kampus IPB Dramaga Bogor
16680, Indonesia
2
Department of Biology, Faculty of Math and Natural Science, IPB
University, Kampus IPB Dramaga Bogor 16680, Indonesia
3
Department of Forest Resources Conservation and Ecotourism,
Faculty of Forestry and Environment, IPB University, Kampus IPB
Dramaga Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The pelung chicken is grouped as the long-crowing

chicken in Indonesia, and it is mainly used for crowing competitions
and exhibition purposes. This study first screened the purity of the
32 pelung long-crowing cocks based on the cock pedigree record
and the sequence of the mtDNA displacement loop (D-loop) region.
From the initial screening of their genetic purity, there were only 20
cocks further evaluated for their acoustic characters based on 2 age
groups: 7-12 months (P1) and 15-20 months (P2) (10 birds each).
The cock songs were recorded with a Sennheiser ME66 microphone
and stored in the Sony PCM D50. The Raven Pro 1.4 program was
used to analyze the cocks song profiles. There were 3 waveforms
and 3 oscillogram regions observed in this study. The P2 produced a
better quality of the song compared to the P1.

Keywords: acoustic, age, long crowing, mtDNA D-loop, pelung

chicken.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 93

94 | International Symposium on Innovative Approaches to Livestock Industry 2025
Eco friendly animal feed resources and nutrition (OP-7)
Moderator : Dr. Wulansih Dwi Astuti
Room 2

Time Abs No. Title Author

12.30-12.45 OP-7-1 Effect of Adding Curcuma xanthorrhiza M. S. Lim, D. A.
to Ram Post Transportaion Diet on Astuti, D.
Physiological Response and Blood Budiono, N. A.
Profile Sholeha, and T. U.
P. Sujarnoko
12.45-13.00 OP-7-2 The Effect of the Combination Boron Y. Armaji, C. M.
(B) Foliar Fertilizer with Mikoseed- Aprelene, L.
Cookies (MSC) on Canavalia ensiformis Abdullah, P. D.
(Jack Bean) Parent Plants to the M. H. Karti, D. A.
Physiological Quality of the Seeds Astuti, and
Produced Nahrowi
13.00-13.15 OP-7-3 Physical Properties and Energy Content N. N. Barkah,
of Different Rumen-Protected Fats from Despal, Y.
Crude Palm Oil Retnani, N. O. H.
Putri, S.
Rahmayuni and R.
D. N. Metania
13.15-13.30 OP-7-4 Performance, Physiological Responses, H. N. Rizqa, R.
and Blood Mineral Profiles in Garut Rachmawati, S.
Ewes Fed Ration with Different Protein Suharti, E. L.
and Energy Levels Aditia, D.A.
Astuti
13.30-13.45 OP-7-5 Optimizing Soybean Protein Protection A. Rosmalia, R.
Through Roasting: Effects of A. Destrisatania, I.
Temperature and Heating Duration on In G. Permana, and
Vitro Fermentability and Digestibility D. Evvyernie
13.45-14.00 OP-7-6 Evaluation of the Nutritional Profile of E. J. Escala, R.
Matoa (Pometia pinnata) Peel-Enriched Martin, and
Corn Silage Nahrowi
14.00-14.15 OP-7-7 Organoleptic and Physical Quality of F. R. Azzahra, I.
Silage as Affected by the Addition of Susanto, R.
Lactic Acid Bacteria and Acacia Tannin Ridwan and A.
Extract Jayanegara
14.15-14.30 OP-7-8 Performance of Pre-weaning Lambs S.I.W. Rakhmani,
Given Starter Feed Containing W. Puastuti, D.
Indigofera zollingeriana Leaf Meal Yulistiani,
Anuraga
Jayanegara

International Symposium on Innovative Approaches to Livestock Industry 2025 | 95

Effect of Adding Curcuma xanthorrhiza to Ram Post
Transportation Diet on Physiological Response and Blood
Profile

M. S. Lim1, D. A. Astuti1, D. Budiono2, N. A. Sholeha2, and T. U.

P. Sujarnoko3*
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Study Program of Veterinary Paramedic, College of Vocational
Studies, Bogor Agricultural University (IPB University), Jalan
Kumbang No. 14, Bogor 16151, Indonesia
3
Study Program of Chemical Analysis, College of Vocational
Studies, Bogor Agricultural University (IPB University), Jalan
Kumbang No. 14, Bogor 16151, Indonesia

E-mail correspondence: [email protected]

Abstract. The transportation process of sheep can induce stress,

affecting their physiological responses, decreasing body weight, and
disrupting blood profiles. Curcuma xanthorrhiza, a traditional
herbal plant, contains curcumin and essential oils. Curcuma can
reduce oxidative stress, boost the immune system, and has
antibacterial and anti-inflammatory properties. This study aimed to
evaluate the effects of Curcuma in the post-transportation diet of
rams on physiological responses and blood profiles. This study used
15 fat-tailed rams aged 12-14 months, with an average body weight
of 19.71±1.97 kg. The experimental design was a randomized block
design with three treatments (T0: Control without Curcuma powder,
T1: complete feed with 1% Curcuma powder, and T2: complete feed
with 2% Curcuma powder) and five replications based on body
weight. The parameters tested included body weight loss,
physiological responses, and blood profiles. Results showed that the
treatment had no significant effect (p>0.05) on physiological
responses and blood profiles, except erythrocytes. However, after
nine days of observation, the recovery time significantly impacts

96 | International Symposium on Innovative Approaches to Livestock Industry 2025

(p<0.05) physiological responses, erythrocytes, leukocytes,
hemoglobin, neutrophils, and creatinine. In conclusion, adding up to
2% Curcuma to the post-transportation diet of rams does not
negatively affect any parameters.

Keywords: blood profile, curcuma, physiological response,

recovery period, transportation

International Symposium on Innovative Approaches to Livestock Industry 2025 | 97

The Effect of The Combination Boron (B) Foliar Fertilizer
with Mikoseed-Cookies (MSC) on Canavalia ensiformis (Jack
Bean) Mother Plants to The Physiological Quality of The
Seeds Produced

Y. Armaji1*, C. M. Aprelene1, L. Abdullah1, P. D. M. H. Karti1,

D. A. Astuti1, and Nahrowi1
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The Canavalia ensiformis was a diversified crop with

high economic value, one of which was as animal feed. To support
optimal cultivation and the ability to produce quality seeds, optimal
doses of fertilization and planting media were important factors in
supporting the growth of plants. This study aimed to evaluate the
physiological quality of Canavalia ensiformis seeds, which were a
harvested product from mother plants, by administering a
combination of different doses of B fertilizer and diameters MSC.
This study used a randomized block design with a 4x3 factorial with
4 replicated blocks based on differences of B fertilizer doses
combined with the diameter of MSC in each block. The B dose: 0,
23, 46, 69 mg L-1 and the diameter of MSC: 4, 5, 6 cm. The P3M2
treatment significantly effect (P<0.05) seed production (3.85 3.85
ton ha-1), 1000-seeds weight (1,471.25 g), and germination (99%).
The highest vigor-index (83%) was in the P4M3 treatment, though
other treatments (P2M3, P3M3, P3M2, P3M1, P4M2) had similar,
non-significant (P>0.05) vigor-index. The combination of 46 mg L-
1 B fertilizer (P3) and 5 cm MSC diameter (M2) improved
germination, vigor-index, 1000-seed weight, and seed production
while also reducing harvest time to 4-months and producing large
Canavalia ensiformis seeds.

98 | International Symposium on Innovative Approaches to Livestock Industry 2025

Keywords: boron, Canavalia ensiformis, mikoseed-cookies, seeds
production, vigor index, weight of 1000 grains.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 99

Physical Properties and Energy Content of Different Rumen-
Protected Fats from Crude Palm Oil

N. N. Barkah1, Despal1, Y. Retnani1*, N. O. H. Putri1, S.

Rahmayuni1, and R. D. N. Metania1
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Rumen-protected fat (RPF) is commonly incorporated

into dairy rations to enhance energy density and improve the fatty
acid profile of milk. The physical properties and energy content of
RPF can vary significantly based on the protection method used,
which impacts ration formulation, handling, and mixing. This study
aimed to compare the physical properties and energy density of RPF
derived from crude palm oil processing, using a randomized block
design with three treatments and four replications: A1 = RPF Ca-
soap, A2 = RPF Prill fat, and A3 = RPF Triglyceride. Results
indicated that different protection methods had a significant effect
on physical properties, including water activity, bulk density, angle
of repose, and particle size (P < 0.05). The gross energy content of
the RPF types were 37.33 MJ/kg for A1, 44.99 MJ/kg for A2, and
46.77 MJ/kg for A3. Despite the differences in protection methods,
all RPF types demonstrated physical properties that were compatible
with handling and mixing processes. Due to their high energy
content, these RPFs are suitable as energy supplements for dairy
rations.

Keywords: crude palm oil, dairy cattle, gross energy, physical

quality, rumen-protected fat.

100 | International Symposium on Innovative Approaches to Livestock Industry 2025

Performance, Physiological Responses, and Blood Mineral
Profiles in Garut Ewes Fed Ration with Different Protein
and Energy Levels

H. N. Rizqa1, R. Rachmawati1, S. Suharti1*, E. L. Aditia2,

and D. A. Astuti1
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor
16680, Indonesia
2
Department of Animal Production Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB
Dramaga, Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. This study aims to analyze performance,

physiological response and blood mineral levels of Garut ewes
fed rations with different protein and energy content. This study
used 20 Garut ewes aged 1-2 years with an average weight of
36.37±4.04 kg. This study used a Block Randomized Design
with 2 treatments and 5 Blocks. The treatments consisted of P0=
grass+commercial concentrate (60:40) with 11% CP and 56.74%
TDN and P1= grass+ standardized concentrate (60:40) with 14%
CP and 61.66% TDN. Variables observed included feed intake,
body condition score (BCS), body weight gain (BWG),
temperature humadity index (THI) value, physiological
response, blood mineral levels, and income over feed cost
(IOFC). Data were analyzed using Independent Sample T-Test.
The results showed that feeding ewes at protein level 14%
increased (P≤0.05) feed intake and BWG in months 2 and 3,
increased (P≤0.05) the blood Ca mineral content and reduced
(P≤0.05) the average afternoon rectal temperature and heart
rate. BCS also significantly affected (P≤0.05) at month 1 and 3
after rearing. The IOFC value in the standardized concentrate
treatment resulted in higher profit. It can be concluded that the
use of standardized concentrates can improve performance,
IOFC and stabilize the level of heat stress in Garut ewes.

Keywords: BCS, blood minerals, garut ewes, performance,

physiological response, standardized concentrate

International Symposium on Innovative Approaches to Livestock Industry 2025 | 101

Optimizing Soybean Protein Protection Through Roasting:
Effects of Temperature and Heating Duration on In Vitro
Fermentability and Digestibility

A. Rosmalia1, R. A. Destrisatania1, I. G. Permana1*, and D.

Evvyernie1
1
Department of Animal Nutrition and Feed Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Soybean (Glycine max L.) is a high-quality protein source

for dairy rations but contains high levels of rumen degradable
protein (RDP), necessitating protection. Producing protected protein
through roasting has shown variable results, particularly in
determining the optimal temperature and duration of heating. This
study aimed to determine the optimal temperature and duration for
protecting soybean protein using the roasting method, observed by
in vitro fermentability and digestibility. A Randomized Block
Design was employed with five treatments: unroasted soybeans
(R1), soybeans roasted at 180°C for 30 min (R2), soybeans roasted
at 180°C for 40 min (R3), soybeans roasted at 200°C for 30 min
(R4), and soybeans roasted at 200°C for 40 min (R5), and four
groups based on rumen sampling. Data were analyzed using analysis
of variance (ANOVA) and, if significant (p<0.05), followed by the
Duncan test. The results showed that the roasting method decreased
NH3, dry matter and organic matter digestibility (DMD and OMD)
while enhancing VFA production, protozoa and bacteria population
(p<0.01). The R4 treatment was optimal for protecting soybean
protein in the rumen, as indicated by a reduction in NH 3 and an
increase in total VFA without significantly decreasing DMD and
OMD.

Keywords: digestibility, fermentability, protected protein, roasting,

soybean.

102 | International Symposium on Innovative Approaches to Livestock Industry 2025

International Symposium on Innovative Approaches to Livestock Industry 2025 | 103
Evaluation of the Nutritional Profile of Matoa (Pometia
pinnata) Peel-Enriched Corn Silage

E. J. Escala1*, R. S. H. Martin2, and Nahrowi2

1
Livestock Research and Development Center, Capiz State
University, Bailan, Pontevedra, Capiz 5802, Philippines
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. This study evaluated the effect of matoa peel inclusion on

the nutritional composition of corn silage. Sixteen packs of 200 g
corn silage, with 0%, 20%, 30% and 40% matoa peel, were analyzed
for pH and nutrient content on day 0 and 21 after ensiling. Results
showed lower pH of 3.62 after 21 days of ensiling, while addition of
20% matoa peel resulted in the lowest pH of 3.90 across inclusion
levels. Highest dry matter was observed on day 0. Although there
was no significant interaction effect on ash content, significant
variation among inclusion levels was observed, with the highest
value at 9.65% in the 0% inclusion. Crude protein (CP) content
showed a significant interaction, with the highest CP in the 20%
matoa peel inclusion on day 0. Significant difference was noted in
the CF, which was highest at the 40% inclusion level. Significant
interaction was found in ether extract (EE), with the highest EE in
the 0% inclusion after 21 days. Nitrogen-free extract (NFE) revealed
significant differences, highest value in 0% matoa peel inclusion.
Incorporating matoa peel into corn silage significantly influenced
pH, CP, CF, and EE, with the 20% inclusion level showing optimal
effects on pH and CP.

Keywords: corn silage, ensiling days, matoa peel, nutritional

composition, pH, proximate analysis.

104 | International Symposium on Innovative Approaches to Livestock Industry 2025

Organoleptic and Physical Quality of Silage as Affected by the
Addition of Lactic Acid Bacteria and Acacia Tannin Extract

F. R. Azzahra1, I. Susanto1, R. Ridwan2, and A. Jayanegara1*

1
Department of Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Research Center for Applied Zoology, Reseacrh Organization for
Life Science and Environment, National Research and Innovation
Agency (BRIN), Cibinong, Bogor 16911, Indonesia

E-mail correspondence: [email protected]

Abstract. The purpose of this study was to determine the effect of

adding lactic acid bacteria (LAB) inoculant and acacia tannin extract
(Acacia mangium) on the organoleptic and physical quaity of forage
silage. A completely randomized design consisting of three factors
with five replications was used in this study. The factors were
addition of lactic acid bacteria (Lactiplantibacillus plantarum)
inoculant, the source of tannin extract from the bark and leaves of
acacia, and the type of forage. Parameters measured were
organoleptic and physical quality of silage, including color, odor,
texture, and presence of fungi. Collected data were analyzed by
analysis of variance and followed by Duncan’s test. Results revealed
that the addition of L. plantarum inoculant had a significant effect
(P<0.05) on the odor and texture of silage. Silage with tannin
significantly (P<0.05) affected the presence of fungi in silage. Silage
with different forage types had a significant effect (P<0.05) on the
color, odor, and texture of silage. In conclusion, LAB and tannin
extract may be combined as additives to maintain the quality of
silage.

Keywords: acacia, Lactiplantibacillus plantarum, organoleptic,

silage, tannin.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 105

Performance of Pre-weaning Lambs Given Starter Feed
Containing Indigofera zollingeriana Leaf Meal

S. I. W. Rakhmani 1,2*, W. Puastuti1, D. Yulistiani1, and A.

Jayanegara2
1
Research Centre for Animal Husbandry, Research Organization for
Agriculture and Food, Indonesian National Research and
Innovation Agency, KST Cibinong
2
Department of Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Survival rate of pre-weaned lamb is typically low,

apparently due to inadequate nutrition derived from maternal milk.
It is therefore essential to enhance the survival by supplementing
pre-weaned lamb with additional nutrition in the form of starter
(creep) feed. This study aimed to evaluate the supplementation of
Indigofera zollingeriana (Iz) leaf meal in creep feeding. The
experiment utilized 18 lambs along with their respective mothers,
and divided into several treatment groups, i.e., creep feeding with Iz
(KKI), without Iz (KK), and a control group (K, exclusively
suckling from the mother). Parameters measured were chemical
composition of the diets, lamb weight gain and milk production,
while ewe weights were also recorded. Results revealed that the
protein content of the starter feed was 34.8% for KK and 34.2% for
KKI. Mortality rates were 0% for KKI and KK, with one lamb
mortality recorded in the K group. Milk production: K:239±70.0
g/d, KK:547±360.4 g/d, KKI:308±195 g/d. The provision of creep
feeding resulted in increased lamb body weights, with weight gains
for KK (12.1±1.44 kg/h/12weeks) and KKI (12.7±2.29
kg/h/12weeks) surpassing those of K (8.82±1.49 kg/h/12weeks). In
conclusion, feeding starter feed to pre-weaned lambs is associated
with higher body weights and has the potential to enhance survival
rates.

106 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Keywords: creep feed, Indigofera zollingeriana, lamb, weight gain.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 107

108 | International Symposium on Innovative Approaches to Livestock Industry 2025
Eco friendly animal feed resources and nutrition (OP-8)
Moderator : Dr. Roni Ridwan
Ballroom IICC 1

Time Abs No. Title Author

12.30-12.45 OP-8-1 Application of Feed Supplement M. Slait, D. M.
Nanocalcium-Vitamin D, or Protease Fassah, and D. A.
Enzymes to Rumen Fermentation Astuti
Characteristics of Fattening Sheep
12.45-13.00 OP-8-2 Effect of Locally Formulated Urea N. S. A. Hanafi, K.
Molasses Multi-nutrient Block Mat, H. C. Harun, H.
Supplementation on Growth and A. Hassim, A. F.
Health Performances of Beef Cattle Mohd Azmi, M.
Mahmud, S. M. Al-
Amsyar, S. H. Sead,
and N. D. Rusli
13.00-13.15 OP-8-3 Agrosilvopastoral Design for Forage Sunardi, H.
Supply Based on Slope Units to Purwawangsa,
Increase Landslide Resilience Supriyanto, and N. R.
Kumalasari
13.15-13.30 OP-8-4 Estrus Characteristics, Percentage of J.A. Yusuf, Patmalia,
Gestation, Hematology Profile, and S. Suharti, M. Baihaqi,
Blood Metabolites in Garut Ewes L. Khotijah
Fed Ration with Different Protein
Levels
13.30-13.45 OP-8-5 Rumen Fermentability Profiles and D. S. Wahyuni, H.
Digestibility of Combination of Herdis, S. Suharti, M.
Camellia sinensis, Hibiscus rosa Surachman, S.
sinensis and Clove oil as Feed Akhadiarto, W. A.
Supplement: In vitro Assay Darmawan, W. Artati,
R. A. Gopar, W.
Negara, S. Martono,
H.A. Parastiwi, R. D.
Purba, P. S. Negoro,
H. A. Sukria, A.
Jayanegara
13.45-14.00 OP-8-6 Probiotic and Coconut Oil S. N. Indah, A.
Supplementation for Colic Mulyana, A. R. Akbar,
Prevention in Horses D. A. Ramadhan,
Nahrowi, A. Kurnia,
Amrozi, B. J.
Widyananta, D. M.
Fassah, B. Triadi, S.
H.Wijaya, A.
Sudarman
14.00-14.15 OP-8-7 Potential Use of Black Soldier Fly Hassim, H.A
Larvae (Hermetia illucens) As an
Alternative Protein In Animal Feed

International Symposium on Innovative Approaches to Livestock Industry 2025 | 109

110 | International Symposium on Innovative Approaches to Livestock Industry 2025
Application of Feed Supplement Nanocalcium-Vitamin D, or
Protease Enzymes to Rumen Fermentation Characteristics of
Fattening Sheep

M. Slait1*, D. M. Fassah1, and D. A. Astuti1

1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. This study aimed to analyze the characteristics of rumen

fermentation in fattening sheep with supplementation of
nanocalcium-vitamin D, bromelain enzyme, and enzyme papain
enzyme. A total of 12 thin-tailed sheep with an initial body weight
of 13.8 ± 1.36 kg was used in this study. This study used a
completely randomized block (CRBD) consisting of four treatments,
namely P0 (pakchong grass 30% + concentrate 70%) as control, P1
(P0 + nanocalcium-vitamin D 5.06 g kgBW -1 day-1), P2 (P0 +
bromelain enzyme 108 mg kgBW -1 day-1), and P3 (P0 + papain
enzyme 108 mg kgBW-1 day-1). Parameters measured included pH
value, protozoa population, total bacterial population, NH 3, total
VFA, partial VFA, and CH4 production in the rumen. The results
showed that the treatment of nanocalcium-vitamin D, bromelain
enzyme, or papain enzyme had no significant effect (p>0.05) on pH
value, protozoa population, total bacterial population, NH 3
concentration, total VFA, partial VFA, and CH 4 production.
Supplementation of nanocalcium-vitamin D, bromelain enzyme, or
papain enzyme in sheep does not interfere with fermentation activity
in the rumen.

Keywords: bromelain, nanocalcium, papain, rumen fermentation,

vitamin.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 111

Effects of Locally Formulated Urea Molasses Multi-nutrient
Block Supplementation on Feed Intake, Growth Performance
and Meat Quality of Beef Cattle in Malaysia

N. S. A. Hanafiah1, K. Mat 1,2, H. C. Harun1,2, H. A. Hassim3,4, A.

F. Mohd Azmi5, M. Mahmud1, S. M. Al-Amsyar1,2, S. H. Sead6,
and N. D. Rusli1,2*
1
Department of Agricultural Sciences, Faculty of Agro-Based
Industry, University Malaysia Kelantan, Jeli Campus,17600 Jeli,
Kelantan, Malaysia
2
Advanced Livestock and Aquaculture Research Group, Faculty of
Agro-Based Industry, Universiti Malaysia Kelantan, Jeli Campus,
17600 Jeli, Kelantan, Malaysia
3
Laboratory of Sustainable Animal Production and Biodiversity,
Institute of Tropical Agriculture and Food Security, Universiti Putra
Malaysia (UPM), 43400 Serdang, Malaysia
4
Department of Veterinary Preclinical Sciences, Faculty of
Veterinary Medicine, UPM, 43400 Serdang, Malaysia
5
Department of Veterinary Preclinical Sciences, Faculty of
Veterinary Medicine, Universiti Malaysia Kelantan. 16100
Pengkalan Chepa, Kelantan, Malaysia
6
FGV Integrated Farming Holdings Sdn Bhd, Kapar Livestock Feed
Mill, 42200 Sungai Kapar Indah, Klang, Selangor.

E-mail correspondence: [email protected]

Abstract. Ruminant production in Malaysia faces significant

challenges due to nutritional deficiencies, affecting growth
performance and overall productivity. To address these issues, this
study evaluated the effects of locally formulated urea molasses
multi-nutrient mineral block (UMMB) supplementation on cattle's
body weight, feed intake, and meat production. An 80-day feeding
trial was conducted at a local beef cattle farm in Tanah Merah,
Kelantan, Malaysia, involving twenty beef cattle, divided into two
age groups: calves (N=10) and adults (N=10). Cattle were randomly
assigned to either a control group (C1=calves and C2=adults)

112 | International Symposium on Innovative Approaches to Livestock Industry 2025

 receiving a basal diet or a treatment group (T1=calves and
T2=adults) receiving the basal diet supplemented with UMMB.
Daily feed and block intake were monitored, and live weights were
recorded biweekly. Post-slaughter carcass percentage, meat quality,
and composition were analysed. Results indicated that UMMB
supplementation enhanced dry matter intake, particularly in adult
cows, without significantly affecting live weight or feed conversion
ratio. Additionally, the treatment groups exhibited a higher carcass
percentage (p<0.05), with no adverse impact on meat pH, nutritional
composition, or quality. These findings suggest that UMMB
supplementation can improve feed intake and meat yield, providing
a practical solution for enhancing local meat production and
supporting small farmers in Malaysia.

Keywords: beef cattle, growth, feed intake, meat production, multi-

nutrient supplement, UMMB

International Symposium on Innovative Approaches to Livestock Industry 2025 | 113

Agrosilvopastoral Design for Forage Supply Based on Slope
Units to Increase Landslide Resilience

Sunardi1,2, H. Purwawangsa2, Supriyanto2,3, and N. R.

Kumalasari2*
1
Study Program of Agrotechnology, Faculty of Agriculture, Nusa
Bangsa University, JL. KH Sholeh Iskandar KM.4 Tanah Sareal
Bogor 16166, Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
3
SEAMEO BIOTROP, Jalan Raya Tajur Km.6 Bogor 16134,
Indonesia

E-mail correspondence: [email protected]

Abstract. The research aimed to investigate the high-risk steep

slopes and design specific vegetation patterns as agrosilvopastoral to
increase landslide resilience and supply animal livestock at
surrounding villages. The research was conducted at Pasir Madang
village, Sukajaya Subdistrict, Bogor Regency, West Java Province.
The village area was impacted by erosion on the valley side
undergoing a deepening process at a former landslide area of
Halimun Salak mountain foot slopes. Data are collected through
aerial photo interpretation by delineating morphological units of old
landslides, slope units, and existing land use. Field surveys were
conducted to (1) verify aerial photo interpretation and (2) identify
and assess existing geomorphological processes. The result showed
that agrosilvopastoral design would stabilize the landslides due to
the tree and crop arrangement based on the morphological units
from the previous landslide. The slope units were classified into
three zones at the highest elevations (>40 o) as the conservation zone,
the second elevation (>30o) as the agrosilvopastoral zone, and the
lowest elevations with gentle slopes as the crop production zone.
The combination of trees and crops especially vetiver across the
slope have different functions in increasing landslide resilience and

114 | International Symposium on Innovative Approaches to Livestock Industry 2025

supporting the forage supply for livestock, especially sheep and
buffalo.

Keywords: agrosilvopastoral zone, animal livestock, forage supply,

landscape design, landslide resilience.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 115

 Estrus Characteristics, Percentage of Gestation,
Hematology Profile, and Blood Metabolites in Garut Ewes
Fed Ration with Different Protein Levels

J. A. Yusuf1, Patmalia1, S. Suharti1*, M. Baihaqi2, and L.

Khotijah1
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department of Animal Production Technology, Faculty of
Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. This research aimed to analyze the effect of different

levels of crude protein (CP) rations on estrus characteristics,
percentage of gestation, hematological profile, and blood
metabolites of Garut ewes. This study used 20 Garut ewes (I1-
I2) with an average body weight of 36.37 ± 4.04 kg and 4
Dorper male sheep. This study used a randomized block design
(RBD) with 2 treatments and 5 groups. The treatments used
were P0= grass+commercial concentrate (60:40) with 11% CP
and P1= grass+standardized concentrate (60:40) with 14% CP.
Variables measured were estrus characteristics, number of
fetuses, percentage of gestation, hematology (erythrocytes,
leukocytes, hemoglobin, hematocrit, and leukocyte
differentiation), and blood metabolites (glucose, triglycerides,
total protein, albumin, blood urea nitrogen (BUN) and
cholesterol). Data were analyzed using an independent sample
T-test. The results showed that the use of ration with protein
level 14% significantly decreased (P<0.05) vulvar temperature
but tended to increase (P<0.1) vulvar mucus. Vulvar color and
shape were relatively similar between treatments. Higher levels
of CP significantly increased (P<0.05) hemoglobin, hematocrit,
total protein, and albumin levels in gestation sheep. Feeding
ration with 14% crude protein can increase the percentage of
successful gestation from 70% to 90% and improve hemoglobin
and hematocrit levels in Garut ewes.

Keywords: blood metabolites, estrus characteristics, Garut

ewes, hematology, standardized concentrate, successful
gestation

116 | International Symposium on Innovative Approaches to Livestock Industry 2025

International Symposium on Innovative Approaches to Livestock Industry 2025 | 117
Rumen Fermentability Profiles and Digestibility of
Combination of Camellia sinensis, Hibiscus rosa sinensis and
Clove oil as Feed Supplement: In Vitro Assay

D. S. Wahyuni1*, H. Herdis1, S. Suharti3, M. Surachman1, S.

Akhadiarto1, W. A. Darmawan1, W. Artati4., R. A. Gopar1, W.
Negara1, S. Martono2, H.A. Parastiwi1, R. D. Purba1, P. S.
Negoro1, H. A. Sukria3, and A. Jayanegara3
Jakaria1, N. R. Kumalasari2, and W. Al Zahra1
1
Research Center for Animal Husbandry National Research and
Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor KM 46,
Cibinong, Bogor 16915, Indonesia
2
Directorate of Laboratory Management, Facilities, Science
Technology Area, National Research and Innovation Agency
(BRIN), Bogor, Indonesia
3
Department Animal Nutrition and Feed Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia
4
Study Program of Nutrition Science and Feed Technology,
Faculty
of Animal Science, IPB University. Jl. Agatis, Kampus IPB
Darmaga, Bogor 16680, West Java, Indonesia

E-mail correspondence: [email protected]

Abstract. The optimal tea by product combination to increase VFA

production was found to be 2 mg/ml plus 0.3 mg/ml of hibiscus leaf
(Supplement 1). This study aimed to assess the effect of a
combination of Hibiscus rosa-sinensis, Camellia sinensis leaf meal,
and clove oil on the fermentability and digestibility of rumen in
vitro. The experiment design used was a complete randomized block
design with 4 treatments and 5 replications. Forage and concentrate
were used as the substrates for the in vitro fermentation at a ratio of
60:40 (% DM). The treatments included the following: A1: forage:
concentrate (60:40) as control, A2: control + Supplement 1, A3:
control + Supplement 1 + 0.02 mg/ml clove oil, A4: control +

118 | International Symposium on Innovative Approaches to Livestock Industry 2025

Supplement 1 + 0.04 mg/ml clove oil. Variables observed were dry
matter digestibility (DMD), organic matter digestibility (OMD),
total volatile fatty acid (VFA) production, ammonia (NH3)
concentration, and total gas production. The result showed that the
addition of clove oil 0.04 mg/ml increased total VFA (p<0.01),
decreased DMD (P<0.01), reduced OMD, and declined total gas
production (P<0.1). NH3 concentrations and pH values showed no
significant effect (P>0.05). In conclusion, rumen fermentation
characteristics may be stimulated by adding clove oil up to 0.04
mg/ml.

Keywords: Camellia sinensis, clove oil, digestibility, Hibiscus

rosasinensis, in vitro, rumen fermentability

International Symposium on Innovative Approaches to Livestock Industry 2025 | 119

Probiotic and Coconut Oil Supplementation for Colic
Prevention in Horses

S. N. Indah1, A. Mulyana2, A. R. Akbar2, D. A. Ramadhan2,

Nahrowi1, A. Kurnia2, Amrozi3, B. J. Widyananta3, D. M.
Fassah1, B. Triadi1, S. H. Wijaya2, and A. Sudarman1
1
Faculty of Animal Science- IPB University
2
Faculty of Mathematics and Natural Sciences- IPB University
3
School of Veterinary and Biomedicine- IPB University
Kampus IPB Dramaga, Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Colic is a prevalent digestive disorder in horses. This

study aimed to prevent colic by incorporating probiotics and
coconut oil into horse feed. Three young mares were fed a 50:50
concentrate-forage diet. Concentrate and forage were given 2 times
a day each. The treatments given were P0 (control) in the form of a
regular ration, P1 with the addition of probiotics of 2% and P2 with
the addition of coconut oil of 4%. The treatments were given
alternately to each mare. After a 10-day adaptation period, each
treatment lasted 14 days. Parameter measurements were carried out
on the last day of each treatment period. The parameters measured
were hematology, blood metabolites, pH, and fecal microbes.
Results indicated that while there were no significant changes in
hematology and blood metabolites, probiotic supplementation
reduced E. coli and lactic acid, leading to an increased pH. This
reduction in acidity suggests a potential decrease in the risk of
acidosis and colic in horses.

Keywords: Coconut oil, colic prevention in horses, digestive health,

probiotics, E. coli reduction.

120 | International Symposium on Innovative Approaches to Livestock Industry 2025

Potential Use of Black Soldier Fly Larvae (Hermetia illucens)
As an Alternative Protein in Animal Feed

Hassim, H.A.

Institute of Tropical Agriculture and Food Security; Faculty of

Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia

E-mail correspondence: [email protected]

Abstract. The combined effects of a growing human population and

increasing demand for sustainable animal protein has led to many
research and development related to black soldier fly larvae (BSFL)
as an alternative protein source in animal feed. Apart from that, the
BSFL also rich in lipids, vitamins and minerals, making them a
good nutrient source for animal feed. The utilization of BSFL
further develops and applies circular economy concepts, using
sustainable, non-polluting and efficient biological process in
converting organic waste into animal feed, biomass and fertilizer.
Additionally, BSFL has the potential to reduce the cost of animal
feed formulations by partially replacing soybean and/or fish meals.
This is one of the primary reasons why insects like BSF are seen as
viable alternative protein sources for animal feeds. Nevertheless, the
use of BSFL in animal feed presents some issues and challenges in
terms of processing (small scale vs large scale), variability and
suitability of organic waste as feedstock and consumer acceptance
towards the use of insects as feed ingredients. However, this should
be considered in the context of the current shortage of protein
sources as animal feed and the nutritional value of BSFL, which has
important research significance for sustainability of livestock
production.

Keywords: black soldier fly larvae, alternative protein, animal feed,

organic waste, circular economy

International Symposium on Innovative Approaches to Livestock Industry 2025 | 121

Animal welfare, health, and environment (OP-9)
Moderator : Edit Lesa Aditia, S.Pt., M.Sc
Room 3

Time Abs No. Title Author

12.30-12.45 OP-9-1 Benchmarking Study of Farmer Practices M. Baihaqi, H.
on Land Sheep Transportation in Nuraini, A.
Indonesia Jayanegara, and
W. Manalu
12.45-13.00 OP-9-2 Productivity of Mini Rex Fancy Rabbits W. Jasman, H.
in a Tropical Environment Nuraini & B.
Brahmantiyo
13.00-13.15 OP-9-3 Addition Moringa Leaf Flour as a Means Salam A, N.
for Treating Anemia in Quail Ulupi, and H.
Maheshwari
13.15-13.30 OP-9-4 Physiological Response and Performance IBAD Vedanta,
of Landrace Pigs at Different VA Mendrofa,
Transportation Duration and R Afnan
13.30-13.45 OP-9-5 The Dairy Cow Shelter Aspects S. Prabowo, A.
Assessment in Smallholder Farmer Yani, M. Arifin,
Bogor Region West Java Indonesia Y. Adinata, A.
Amir, I.
Prihantoro
13.45-14.00 OP-9-6 Hematology and Metabolites in Boer- Y. Pujiawati, D.T.
Jawarandu Doe with Flushing Ration Rahmi, and L.
Containing Calcium Soap and Jackfruit Khotijah
Leaf Extract

122 | International Symposium on Innovative Approaches to Livestock Industry 2025

Benchmarking Study of Farmer Practices in Sheep Land
Transport in Indonesia

M. Baihaqi1*, H. Nuraini1, A. Jayanegara2, and W. Manalu3

1
Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
3
Division of Physiology, School of Veterinary Medicine and
Biomedical Sciences, IPB University, Jl. Agatis Kampus IPB
Dramaga, Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The transportation of sheep is a critical factor that can

induce stress and impact their production performance. In Indonesia,
detailed information regarding the practical conditions of sheep
transport is limited. This study aimed to identify the transportation
conditions of sheep during overland journeys across the region. The
research involved the survey of twenty trips of 1204 head of sheep
originating from four different farms. The majority of vehicles
utilized for these trips were pickups equipped using three decks
(88%) with space allowance was 0.15±0.01 m 2/head. The duration
of the trips varied, with 1.84±1.71 hours for short journeys and
12.52±2.78 hours for long journeys. A significant finding was that
the weight loss due to transportation of short journeys was
2.40±1.04% compared to the long journey, which was 10.81±3.79%
of initial body weight (P<0.05). The proportion of lame sheep after
transportation for short and long trips were 3.79±0.95% and
9.37±3.70%, respectively. This study concludes that the
transportation conditions of sheep in Indonesia result in a high body
weight loss, indicating a need for improved transportation practices
to reduce stress and its associated negative impacts on animal
welfare and performance.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 123

 Keywords: body weight loss, land transportation, sheep, survey

124 | International Symposium on Innovative Approaches to Livestock Industry 2025

Performance of Mini Rex Rabbits Raised in a Tropical
Environment

W. Jasman1, H. Nuraini2*, and B. Brahmantiyo3

1
Postgraduate School of Animal Production and Technology,
Faculty of Animal Science, IPB University
2
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
3
Research Center for Animal Husbandry, Research Organization for
Agriculture and Food, National Research and Innovation Agency,
Cibinong Science Center, Jl. Raya Jakarta-Bogor, Cibinong 16915,
Indonesia

E-mail correspondence: [email protected]

Abstract. Minirex rabbits come from subtropical areas, so

evaluating their performance in Indonesia is interesting. The
purpose of this study is to evaluate the performance of minirex
rabbits in tropical climate areas of Indonesia. The study was
conducted at several farms located in the Bogor District. The
samples used were 52 minirex rabbits and 40 rex rabbits as a
comparison. The variables observed were production performance
through morphometric measurements, physiological descriptions of
the body of minirex fancy rabbits and microclimate data on the
farm. Body size data were analyzed using General Linear Models
using SAS ver.9.4 (SAS 2020), and physiological data were
analyzed descriptively. The results showed that the average body
weight of male mini rex rabbits was 1547.37 ± 316.99 g and female
mini rex rabbits was 1743.10 ± 426.54 g; this value is in accordance
with the standard body weight of mini rex rabbits, which ranges
from 1.7-2 kg. The results showed that mini rex rabbits had
morphometric characteristics that were significantly different
(p<0.05) compared to rex rabbits. At an average environmental
temperature of 28.36 oC and humidity of 64.86%, mini rex rabbits

International Symposium on Innovative Approaches to Livestock Industry 2025 | 125

 showed a physiological response of thermal heat index of 25.70 and
was classified as normal.

Keywords: mini rex, morphometrics, performance, physiological

response, tropical environment.

126 | International Symposium on Innovative Approaches to Livestock Industry 2025

Addition Moringa Leaf Flour as a Means for Treating Anemia
in Quail

A. Salam1*, N. Ulupi1, and H. Maheshwari2

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department of Physiology, Faculty of Veterinary Medicine, IPB
University, Jl. Agatis Kampus IPB Dramaga, Bogor 16680,
Indonesia

E-mail correspondence: [email protected]

Abstract. Raising quail in tropical areas will withstand the

challenges of high environmental temperatures which can cause
anemia. Moringa with its high iron content is expected to be able to
overcome this condition. The research aims to analyze quail red
blood cells as an indicator of anemia by adding Moringa leaf flour to
the feed. The study used 120 quail with a Completely Randomized
Design (CRD) consisting of 4 levels of treatment with the addition
of Moringa leaf flour to the feed and 3 replications, namely P0
(control), P1 (2.5%), P2 (5%) and P3 (7.5 %). The parameters
observed included the number of erythrocytes, hematocrit,
hemoglobin, erythrocyte index (MCV, MCH, and MCHC), initial
and final body weight. The results of the red blood cell analysis
obtained were below the normal range, however the addition of
Moringa leaf flour was able to increase the number of red blood
cells in quail experiencing heat stress so that the risk of anemia at a
higher level could be reduced. Reduces and can improve
physiological conditions and support weight gain. The addition of
Moringa leaf flour to feed at a level of 5% showed the best results in
reducing anemia and increasing body weight gain.

Keywords: anemia, iron, moringa, quail, red blood cells.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 127

Physiological Response and Performance of Landrace Pigs at
Different Transportation Duration

I. B. A. D. Vedanta1, V. A. Mendrofa1*, and R. Afnan1

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Livestock transportation is the process of moving

livestock from one place to another for distribution purposes. The
transportation has a direct impact on livestock themselves, including
livestock experiencing stress due to environmental temperature,
changes in physiological responses and reduction in body weight.
This study aimed to analyze the physiological response and
performance of landrace pigs at different transportation durations.
The data were analyzed using CRD (Completely Randomized
Design) with three different time treatments of 1 hour, 2 hours and 3
hours on heart rate, respiratory rate, rectal temperature and body
weight. Additionally, descriptive analysis is used for microclimatic
data and physiological response reconditioning. The experiment
results showed that the pulse rate and body weight of landrace pigs
after loading and before unloading showed different results between
treatments. The mean of landrace body weight before the
transportation was 114.34 kg, while the mean of landrace body
weight after the transportation was 111.85 kg. The results of
landrace pig reconditioning data concluded that it did not decrease
significantly within one hour. Transporting pigs can be carried out
with an optimal duration of 2 hours.

Keywords: body weight, landrace pigs, microclimatic,

physiological response, transportation.

128 | International Symposium on Innovative Approaches to Livestock Industry 2025

The Dairy Cow Shelter Aspects Assessment in Smallholder
Farmer Bogor Region West Java Indonesia

S. Prabowo1*, A. Yani1, M. Arifin1, Y. Ananta2, A. Amir2, and I.

Prihantoro3
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
3
Research Center for Animal Husbandry, National Research and
Innovation Agency of Indonesia, Cibinong Science Center, Jalan
Raya Jakarta – Bogor, Cibinong, Bogor 16915
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Housing factors significantly impact the optimization of

dairy cattle production performance, particularly in smallholder
farms in tropical regions. This study was conducted to assess dairy
farm housing in Bogor, West Java, Indonesia, particularly in
smallholder farms. The Dairy NZ dairy cow housing checklist
evaluated the housing aspects of 15 barns classified as smallholders.
The questionnaire included three main data groups: (1) livestock
conditions, (2) housing facilities, and (3) calving and quarantine
pens. The questionnaire also included general farm information.
Purposive sampling was used to pick the targeted pens, and
descriptive analysis was performed on the collected data. According
to the findings of the field inspection, most of the farms in Bogor
had tie-pen systems, gable roofs, concrete floors, and tail-to-tail
pens, with an average ownership scale of 20.3 cows. An average
score of 13.1 was deemed suitable for livestock conditions, while 1.9
was considered unacceptable. Housing facilities had an average
score of 9.8 for tolerable and 5.7 for intolerable. Comparatively, 4.5
quarantine and calving enclosures were allowed, and 10.5 were not.
The priority order for improving aspects of housing that must be

International Symposium on Innovative Approaches to Livestock Industry 2025 | 129

 improved promptly in the dairy shed in the Bogor area is the aspect
of calving and quarantine pens, housing facilities, and, finally, cow
status conditions as the denouement and final postulate based on the
principle of seriousness.

Keywords: cow comfort, dairy cattle housing, evaluation,

smallholder farmer, tropic area.

Hematology and Metabolites in Boer-Jawarandu Doe with

Flushing Ration Containing Calcium Soap and Jackfruit Leaf
Extract

Y. Pujiawati1*, D. T. Rahmi2, and L. Khotijah2

1
Research Center for Animal Husbandry, National Research and
Innovation Agency, Kawasan Sains dan Teknologi (KST) Soekarno,
Jl. Raya Jakarta-Bogor KM 46, Cibinong, West Java 16911,
Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence:
[email protected]

Abstract. This study aimed to optimize the prolific traits of goats

using polyunsaturated fatty acids (PUFA) and jackfruit leaf extract.
Calcium soap (Ca-Soap) production was used to protect PUFA from
fermentative digestion. However, PUFA and plant extracts can
influence inflammatory mechanisms and oxidative stress, making it
essential to evaluate the hematological profile and blood metabolite
status in does fed a diet containing unsaturated fatty acids and
jackfruit leaf extract. The experimental design used was a
randomized block design with 2 treatments and 5 blocks, divided
into two phases: luteal and follicular. Data were analyzed using
ANOVA, and if significant differences were found, Duncan’s
multiple range test was conducted. The variables measured included
nutrient intake, hematology, and blood metabolites. The use of Ca-

130 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Soap and jackfruit leaf extract did not affect nutrient intake,
hematocrit, hemoglobin, RBC, WBC, and WBC types in the luteal
phase, as well as glucose, triglycerides, and albumin levels (P>0.05).
However, this treatment influenced (P<0.05) the percentage of
monocytes and basophils in the follicular phase, as well as
cholesterol levels in both phases. In conclusion, the use of Ca-Soap
and jackfruit leaf extract does not negatively impact the
hematological profile and can lower plasma cholesterol levels in
both phases.

Keywords: cholesterol, follicular, luteal, plant extract,

polyunsaturated fatty acids.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 131

132 | International Symposium on Innovative Approaches to Livestock Industry 2025
Animal welfare, health, and environment (OP-10)
Moderator : Dr. Arif Darmawan
Room 4

Time Abs No. Title Author

12.30-12.45 OP-10-1 Evaluation of The Application of Good I. R. Purnama, R.
Farming Practice (GFP) of Broiler Afnan, and L.
Business at Palasah Farm Mitra Pt Cyrilla
Ciomas Adi Satwa
12.45-13.00 OP-10-2 The Chemical Properties of Eco-Print E. Nirmalawati,
Leather-Tanned with Vegetable Tanning A.
Agent: Gambier Pertiwiningrum,
and M. Z. Abidin
13.00-13.15 OP-10-3 Analysis of Factors Causing Death of L. Cyrilla, S.
Pigs Due to African Swine Flu Outbreak Sinaga, G. F.
in Toba District, North Sumatra Province Sipayung
13.15-13.30 OP-10-4 Screening, Morphological and R. H. Ash
Biochemical Characterization of a Shiddiq, C.
Cellulose-Degrading Bacterium isolated Budiman, H. Asis,
from Horse Feces (Equus caballus) R. Razali and E.
Taufik
13.30-13.45 OP-10-5 Evaluation of Feeding Behaviour and Rosita, H. A.
Palatability of Domestic Cat (Felix Hassim, L.
Catus) on Locally Formulated Chicken- Maylina, D. A.
Based Wet Pet Food Containing Astuti
Different Flavor

International Symposium on Innovative Approaches to Livestock Industry 2025 | 133

Evaluation of The Application of Good Farming Practice
(GFP) of Broiler Business at Palasah Farm Mitra PT Ciomas
Adi Satwa

I. R. Purnama1*, R. Afnan1, and L. Cyrilla1

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The world of poultry, which is increasingly popular

among the public, with the scale of a household business, continues
to grow in various regions, meanwhile large-scale businesses are
also growing and are able to run their business more efficiently. The
purpose of this study was to determine the application of Good
Farming Practice (GFP) carried out in the broiler business at Palasah
Farm and knowing the value of broiler production performance
based on Feed Conversion Ratio (FCR), Performance Index (IP),
Depletion. The research method used is a case study by collecting
limited primary data. The results of the data were analyzed using
descriptive analysis methods and SWOT analysis. Variables
observed include evaluation of aspects of advice, aspects of the
production process, aspects of environmental preservation and
aspects of supervision. The implementation of Good Farming
Practice aspects has been carried out very well, achieving a GFP
performance score of 91.87% and an achievement of a Performance
Index (IP) of 449.

Keywords: broiler, depletion, good farming practice (GFP), feed

conversion ratio (FCR), performance index (IP).

134 | International Symposium on Innovative Approaches to Livestock Industry 2025

The Chemical Properties of Eco-Print Leather-Tanned with
Vegetable Tanning Agent: Gambier

E. Nirmalawati1, A. Pertiwiningrum1*, and M. Z. Abidin1

1
Faculty of Animal Science, Gadjah Mada University, Yogyakarta
55281, Indonesia

E-mail correspondence: [email protected]

Abstract. Tanning materials in leather production usually use

materials that are not environmentally friendly such as chrome. To
implement clean production in the leather tanning process, the use
of vegetable tanning is starting to be widely studied, one of which is
gambier. This research aims to evaluate the effect of various levels
of ingredients tanner vegetable gambier on the chemical properties
of eco-print-tanned sheep leather. The treatment consists of five
variations of tanner ingredients (vegetable tanning, gambier) 10%,
15%, 20%, 25%, and 30%. The variables measured include fat
content, ash content and water content. The results showed that the
level of gambier as vegetable tanning had a significant effect on fat
content, but had no significant effect on ash content and water
content. The highest fat content was found in the 30% tanning
treatment (18.93 ± 0.7%) while the ash content and water content
did not show significant differences between treatments, with
average values of 1.82-1.94% and 15.33- 18.00%. This research
concluded that the utilization of gambier as a vegetable tanner at
various levels affects the fat content of sheep leather, but had no
significant effect on the ash content and water content.

Keywords: chemical properties, eco-print, gambier, sheep leather,

vegetable tanning.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 135

Analysis of Factors Causing Death of Pigs Due to African
Swine Flu Outbreak in Toba District, North Sumatra
Province

L. Cyrilla1*, S. Sinaga2, and G. F. Sipayung3

1
Faculty of Animal Science IPB University, Jl, Agatis Kampus IPB
Dramaga Bogor
2
Faculty of Animal Husbandry Pajajaran University, Jalan Raya
Bandung Sumedang KM 21, Jatinangor
3
Dinas Pertanian Kabupaten Toba Jalan Pertanian No . 1 Hutabulu-
Mejan Balige

E-mail correspondence: [email protected]

Abstract. This study aims to analyze the factors causing livestock

death in pig farms due to African Swine Flu (ASF) outbreak in Toba
District, North Sumatra Province. The research was conducted at a
community pig farm in Toba District, North Sumatra Province. Data
collection was carried out from May to July 2022. Data collection
was carried out by interviewing farmers, as well as conducting
direct observations in the pens of pig farms in Toba Regency. Sixt
five farmers were selected as respondents in this study. Factors
causing the death of pigs were analyzed using logistic regression.
The dependent variable used is pigs infected with ASF (dead pigs)
with a value of 1, and a value of 0 if there are no pigs infected with
ASF. Variables that have a significant influence on the number of
deaths of pigs in smallholder farms in Toba Regency are: farmer's
education level, main occupation of the farmer, location of pens,
distance of pens from other farmers' pens, housing systems, sanitize
goods that will enter the pen, use of disinfectants, origin of male or
boar, type of feed given, feed processing before being given to pigs,
and carrying out fumigation.

Keywords: African Swine Flu, outbreaks, pig death’ factors, small

pig farms.

136 | International Symposium on Innovative Approaches to Livestock Industry 2025

Screening, Morphological and Biochemical Characterization
of a Cellulose-Degrading Bacteria Isolated from Horse Feces
(Equus caballus)

R. H. A. Shiddiq1, C. Budiman1*, H. Asis2, R. Razali2, and E.

Taufik1
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Biotechnology Research Institute, Universiti Malaysia Sabah, Jl
UMS 88400, Kota Kinabalu, Sabah, Malaysia

E-mail correspondence: [email protected]

Abstract. Cellulase is a widely developed enzyme for industrial

applications, produced by various sources, including bacteria. While
numerous bacteria are known to produce cellulose-degrading
enzymes (CDE), further exploration is needed to discover unique
and highly active CDEs. This study focuses on screening,
identifying, and characterizing cellulose-degrading bacteria (CDB)
from horse feces, which are considered an excellent source for such
bacteria. Fecal samples were collected from Megastar Horse Farm in
Batu, Malang, and screened on carboxymethyl cellulose (CMC)-
containing agar, incubated at 37 °C. The colonies that formed halo
zones on the agar were isolated, and their genomic DNA was
extracted for 16S rRNA gene amplification. Sequence analysis
revealed that the isolate showed over 98% similarity to B.
amyloliquefaciens SLBD and was subsequently labeled as B.
amyloliquefaciens SLBD. Gram staining and scanning electron
microscopy confirmed that B. amyloliquefaciens SLBD is Gram-
positive with a rod-shaped morphology. B. amyloliquefaciens SLBD
also grew optimally in Luria-Bertani (LB) medium at 37 °C. The
CDE activity of this strain was assessed using its extracellular
fraction against CMC and mannan oligosaccharides (MOS),
revealing a substrate-dependent activity profile, with CMC being the
preferred substrate.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 137

 Keywords: Bacillus amyloliquefaciens SLBD, cellulolytic activity,
cellulose producing bacteria, horse feces.

138 | International Symposium on Innovative Approaches to Livestock Industry 2025

Evaluation of Feeding Behaviour and Palatability of Domestic
Cat (Felix Catus) on Locally Formulated Chicken-Based Wet
Pet Food Containing Different Flavor

Rosita1*, H. A. Hassim2, L. Maylina3, and D. A. Astuti4

1
Master's student Department of Animal Nutrition and Feed
Science, Faculty of Animal Science, IPB University, Bogor,
Indonesia, 16680
2
Veterinary Medicine School IPB University Bogor, Indonesia,
16680
3
Institute of Tropical Agriculture and Food Security, UPM
Malaysia;
4
Department of Nutrition and Feed Technology, Faculty of
Animal Science, IPB University, Bogor, Indonesia, 16680

E-mail correspondence: [email protected]

Abstract. The dominance of imported products in Indonesia's pet

food industry necessitates the development of local alternatives.
This study evaluated feeding behaviour and palatability in domestic
cats (Felix catus) using a locally formulated chicken-based wet pet
food. A Randomized Group Design was used with three treatments:
control food, chicken-flavored food, and fish-flavored food. Pet
food was given twice daily for sixty days based on daily intake
requirements. The fish-flavored food had the highest palatability
score (4.88), followed by chicken-flavored (4.71) and control (4.33).
Total dry matter consumption was highest for the fish-flavored food
(85.35 g), followed by chicken-flavored (70.77 g) and control (38.87
g). Protein consumption mirrored these results. Arachidonic acid
content was highest in the control food (0.030%), followed by fish-
flavored (0.028%) and chicken-flavored (0.020%). Cats fed chicken
and fish-flavored diets gained more weight, while those on the
control diet lost weight. Cats showed quicker responses and higher
consumption rates for the flavored foods, and no digestive issues
such as diarrhea were observed during the study period, suggesting
that these foods do not cause distress to the cats' digestive tract. The

International Symposium on Innovative Approaches to Livestock Industry 2025 | 139

 chicken-based local wet pet food formulation is well-accepted by
domestic cats and shows potential as a local alternative in Indonesia.

Keywords: behavior, cat, flavor, pallatability, pet food.

140 | International Symposium on Innovative Approaches to Livestock Industry 2025

 POSTER PRESENTATION

Abs No. Title Author

PP-01 Nano-Emulsified Black Soldier Fly Ratih Dewanti, Ronny Martien,
(Hermetia illucens) Oil Concerning Bambang Ariyadi, and Zuprizal
Breast Meat Fatty Acids of Broilers
PP-02 A Survey of Cattle Health in Village L. N. Oo, S. H. Lwin, M. T.
Farms in Pakokku and Natmauk Naung, B. Hein, A. J. D.
Townships of Magway Region, Campbell, M. T. Htun, and M.
Myanmar Sriasih
PP-03 Reproductive Performance of Y Yanti, A Pramono, J Riyanto, K
Friesian Holstein Cross in Small Sari, L N Nisa, Z H Abdurrahman
Scale Farmer in Boyolali Indonesia
PP-04 Chemical Compositions of Black N. F. A. Jakfar , H. A. Hassim,
Soldier Larve, and Effects on MH Kamalludin, E.S Jamein, and
Nutrient Digestibility in Broiler N. Ramachandran
Chickens
PP-05 Effects of Feeding Agricultural By- S. A. A. Fadzlin, A. B. Zuki, M.
Products on Milk Composition of Zamri-Saad, N. M. Norhariani, A.
Dairy Buffaloes at Early Stage Salleh, and H. A. Hassim
Lactation
PP-06 Comparison on Digestibility of Gyue Gyue, N. K. Hline, M. T.
Different Pulse Crop Residues in Naung, H. Myint, K. S. Mu, L. N.
Goats Oo, Dahlanuddin, and M. T. Htun
PP-07 Production Characteristics of G. T. Prilyadi, H. Nuraini, and R.
Slaughter Cattle in Garut Regency Priyanto
PP-08 Zeolite/Copper Oxide Granules as Z. Abidin, H. Salsabila, Z. T.
Antibacterial Water Filter to Prevent Maulitia, P. N. Angelina, and I. I.
Pathogenic Bacterial Contamination Arief
in Laying Hens Farm
PP-09 Chromosome Assessment of Bali Jakaria, M. F. Ulum, Sutikno, and
Cattle and Their Hybrids Using the R. Priyanto
G-Banding Technique
PP-10 Blood Metabolites Profile, L. Khotijah, S. Munggaran, Y.
Physiological Response, and Estrus Adisti, K. Komalasari, D. M.
Characteristics of Composite Fassah, K. Komalasari, A.
Sumatera Ewes Fed Flushing Ration Tarigan, and D. A. Astuti
PP-11 In Ovo Feeding of Pegagan Leaf A. Darmawan, R. Krisnan, I. N.
(Centella asiatica) Extract Improves Fajri, Y. Artika, M. A.
Hatchability without Impairing Lusbiantoro, I. Wijayanti, R. K.
Physical Quality of Sentul Chicks Rusli, and C. Hidayat
PP-12 Production of Functional Forage: N. R. Kumalasari, W. S. Mulyati,
Gynura procumbens S. Lestari, A. Rosalia, and A. U.
Syahidah
PP-13 Evaluation of Anti-Methanogenic M. S. Daulai, I. Wijayanti, Y.
Compound and Phytogenic Extract Retnani, and S. Toshisada

International Symposium on Innovative Approaches to Livestock Industry 2025 | 141

 Abs No. Title Author
of Moringa oleifera on Ruminal
Methane Production
PP-14 Marinades Application to Reduce Y. K. Silaban, T. Suryati, and Z.
Malondialdehyde Compound and Wulandari
Increase Antioxidant Activity in
Fried Chicken
PP-15 Association of Alcohol K. Listyarini, F. Muharram, and
Dehydrogenase 1C Gene with A. Gunawan
Minerals in Sheep
PP-16 The Body Size and Body Shape of R. H. Mulyono, S. Darwati, C.
Adult Pelung, Sentul, Kampung, and Sumantri, and K. Listyarini
IPB D1 Chickens
PP-17 Evaluating the Efficacy of Panasonic R. Adiyoga, I. I. Arief, N. Ulupi,
WPS in Enhancing Layer Chicken Z. Abidin, I. Komala, and I. G.
Performance Permana
PP-18 Analysis of Human Resources in M A Nabil, L Cyrilla, I Komala,
Tajur Halang Village Dairy Farm for Yumita
Agrotourism Development
PP-19 Smart Microclimate Control System I Komala, M Fayruz, A Yani, MT
using Internet of Things (IoT) on Mubarrok, YB Gampur
Physiological Responses of the
Dairy Cow

142 | International Symposium on Innovative Approaches to Livestock Industry 2025

Nano-Emulsified Black Soldier Fly (Hermetia illucens)
Oil Concerning Breast Meat Fatty Acids of Broilers

R. Dewanti1, 2, R. Martien 3, B. Ariyadi4, and Zuprizal2

1
Department of Animal Production, Faculty of Animal Science,
Universitas Sebelas Maret, Surakarta, Indonesia
2
Department of Animal Nutrition and Feed Science, Faculty of
Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3
Department of Pharmaceutics, Faculty of Pharmacy, Universitas
Gadjah Mada, Yogyakarta, Indonesia
4
Department of Animal Production, Faculty of Animal Science,
Universitas Gadjah Mada, Yogyakarta, Indonesia

E-mail correspondence: [email protected]

Abstract. In the antibiotic-free era, traditional antibiotics have been

suggested as alternatives to antibiotic-based growth promoters.
Among the various methods, self-nano-emulsifying drug delivery
systems (SNEDDS) are increasingly utilized to improve the
bioavailability of oils containing essential substances. In this study,
we evaluated the effects of black soldier fly oil (BSFO) SNEDDS in
chicken drinking water on breast meat fatty acids of broiler. We
divided 225 male Indian River strain broiler chickens into five
treatment groups, each consisting of five replicates. The chickens
were reared from 0–35 days of age in a controlled environmental
housing system. The BSFO SNEDDS was administered via drinking
water. Treatments included ordinary drinking water (P1), bacitracin
(P2), 10 mL/L (P3), 20 mL/L (P4), and 30 mL/L (P5) BSFO
SNEDDS. The observed parameters included breast meat fatty
acids. Data were analyzed using analysis of variance (ANOVA) and
Duncan’s test. The results indicated that administering BSFO
SNEDDS via drinking water increased the breast meat methyl
laurate (P < 0.001) 0.94%, increased methyl palmitate (P < 0.05)
26.34% and methyl palmitoleate (P < 0.1) 0.44%. BSFO SNEDDS
in drinking water can improve the quality of broiler chicken meat
and reduce synthetic antibiotic residues.

Keywords: black soldier fly oil, broiler, fatty acids, lauric acid,
SNEDDS.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 143

A Survey of Cattle Health in Village Farms in Pakokku and
Natmauk Townships of Magway Region, Myanmar

Lwin Naing. Oo1, S. H. Lwin2, M. T. Naung2, B. Hein2, A. J. D.

Campbell3, M. Sriasih1, and M. T. Htun2
1
Faculty of Animal Science, University of Mataram, Lombok,
Indonesia
2
University of Veterinary Science, Yezin, Nay Pyi Taw, Myanmar
3
Faculty of Veterinary & Agricultural Sciences, University of
Melbourne, 250 Princes Highway, Werribee, Victoria, Australia

E-mail correspondence: [email protected]

Abstract. This study aimed to assess the health status and evaluate
the haematological parameters according to the age of cattle in
village farms in the Magway region, Myanmar. A cross-sectional
study was conducted in 10 geographically representative villages in
each township, with five households were randomly selected per
village. Data were obtained through a formal questionnaire on
animal ownership, and blood samples were collected from 1 young
and 1 adult per household. Then, syndromic observations were
recorded to look for 8 different syndromes. The haematological
parameters were measured using a Neubauer type of
Haemocytometer, micro-centrifuge and refractometer. Foot and
Mouth disease was the most frequently reported health issue in the
study area. The most occurrence health syndromes was ill-thrift,
which was greater in adults in Pakokku whereas the gastrointestinal
was greater in young cattle in Natmauk. The mean value of red
blood cells was significantly higher in young cattle. The mean value
of haemoglobin and white blood cells showed no significant
interaction between age. The lower pack cell volume was greater in
adults, whereas the lower total protein value was found in young
cattle in both townships. These findings may be helpful in the
diagnosis and assessment of diseased animals in the future.

144 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Keywords: Cattle health, Village farm, Haematological parameters,
Magway Region.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 145

Reproductive Performance of Friesian Holstein Cross in Small
Scale Farmer in Boyolali Indonesia

Y. Yanti1, A. Pramono1, J. Riyanto1, K. Sari1, L. N. Nisa1, and Z.

H. Abdurrahman2
1
Department of Animal Science, Universitas Sebelas Maret, Jalan Ir.
Sutami No. 36A Kentingan Surakarta 57126, Indonesia
2
Animal Science Program Study, Universitas Boyolali, Boyolali
57315, Indonesia

E-mail correspondence: [email protected]

Abstract. This study aims to obtain information on the reproductive

performance of Friesian Holstein Crossbreed dairy cattle in
Mojosongo and Musuk District, Boyolali Regency. The method
used in data collection is a survey and the sampling technique used
purposive sampling. Data collection uses interviews. The number of
respondents used was 42 dairy farmers from Mojosongo and 40
dairy farmers in Musuk District. All the respondents must have
Friesian Holstein Crossbreed (FH><local cattle) cow which two
times calving. All data observed were analyzed using t-test. The
result showed that the Friesian Holstein crossbreed cow in Musuk
District and in Mojosongo District have no difference in all
parameters observed, except in Service per Conception (S/C). The
age of first mating was 18.5-20.1 months. Age of first calving was
29.1-30.1 months. Post Partum Mating was 65.5-71.5 days. S/C in
Mojosongo District was higher than that S/C in Musuk District.
Days Open was 87.9-98.8 days. Calving Interval was 12.3-12.5
months. Calf’s age at weaning was 3.8-4.1 months. Based on the
results of the study, the reproductive performance of dairy cows in
Mojosongo and Musuk District, Boyolali Regency is classified as
good, but the weaning age still needs to be improved.

Keywords: calving interval, Friesian Holstein crossbreed,

reproductive performance, service per conception, small-scale
farmer.

146 | International Symposium on Innovative Approaches to Livestock Industry 2025

Chemical Compositions of Black Soldier Larvae, and Effects
on Nutrient Digestibility in Broiler Chickens

N. F. A. Jakfar1 , H. A. Hassim*1,2 , M. H. Kamalludin*1,3 , E. S.

Jamein1, and N. Ramachandran4
1
Institute of Tropical Agriculture and Food Security, Universiti
Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
2
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia.
3
Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia.
4
BioLoop Sdn Bhd, Desa Sri Hatamas Kuala Lumpur, 50480
Wilayah Persekutuan, Malaysia.

E-mail correspondence: [email protected] /

[email protected]

Abstract. This study analyzed the nutritional properties, apparent

ileal digestibility (AID), and apparent metabolizable energy (AME)
of broiler chickens fed with black soldier fly larvae (BSFL). The
nutritional composition of BSFL was determined through proximate
analysis, while amino acid and mineral contents were assessed using
high-performance liquid chromatography (HPLC) and atomic
absorption spectroscopy, respectively. Gross energy (GE) and AME
were measured using a bomb calorimeter, and titanium dioxide
(TiO2) was used to calculate AID. The effects of BSFL on crude
protein (CP), crude fiber (CF), ether extract (EE), dry matter (DM),
amino acid, and mineral digestibility, along with AME, were
examined in a study involving 30 broiler chickens across two
treatment groups: BSFL and soybean meal (SBM). Results indicated
that BSFL is a source of essential macro and micronutrients. The
AME values for BSFL and SBM were 3999.2 and 3796.8 kcal/kg,
respectively. AID results showed significant differences in
digestibility, with BSFL diets having higher AID values for CP and
EE compared to SBM. In conclusion, BSFL is a viable alternative
protein source for broiler chickens, providing comparable or
superior digestibility of key nutrients, including protein, fat, and
essential minerals, compared to soybean meal.

Keywords: apparent ileal digestibility, apparent metabolisable

energy, broiler, black soldier fly larvae, soybean meal

International Symposium on Innovative Approaches to Livestock Industry 2025 | 147

Effects of Feeding Agricultural By-Products on Milk
Composition of Dairy Buffaloes at Early Stage Lactation

S. A. A. Fadzlin1, A. B. Zuki2, M. Zamri-Saad2, N. M.

Norhariani2, A. Salleh2, and H. A. Hassim*1,2
1
Institute of Tropical Agriculture and Food Security, Universiti
Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia

E-mail correspondence: [email protected]

Abstract. This study examines the impact of feeding agricultural

by-products (PKE, wheat pollard, rice bran, and soy hull meal) on
the milk composition of dairy buffaloes during early lactation.
Fourteen female Murrah buffaloes were randomly assigned to a
treatment group (n=7), which received 5.0 kg of formulated feed
plus Napier grass, or a control group (n=7), which received only
Napier grass with minimal supplementation. The study lasted three
months, covering early lactation. Milk samples were collected mid-
experiment period and analyzed for their composition. Results
indicated that the crude protein content of the formulated feed
(21.95 ± 0.75%) was significantly higher (p < 0.05) than that of the
farm’s feed (17.55 ± 0.5%). The formulated feed also had higher
gross energy (355.50 ± 3.5 MJ/kg) compared to the farm’s feed
(315.50 ± 5.5 MJ/kg), though this difference was not statistically
significant. The treatment group’s milk fat content (5.50 ± 0.75%)
was significantly higher (p < 0.05) than that of the control group
(3.36 ± 0.41%). No significant differences were found in milk
protein or casein content between groups. Inclusion of agricultural
by-products improved milk quality without adverse effects on the
buffaloes.

Keywords: Dairy buffaloes, agricultural by-products, nutritional

composition, milk composition, milk quality.

148 | International Symposium on Innovative Approaches to Livestock Industry 2025

Comparison on Digestibility of Different Pulse Crop Residues
in Goats

Gyue Gyue1, N. K. Hline1, M. T. Naung1, H. Myint1, K. S. Mu2,

L. N. Oo3, Dahlanuddin3, and M. T. Htun1
1
Department of Physiology and Biochemistry, University of
Veterinary Science, Myanmar
2
Department of Animal Nutrition, University of Veterinary Science,
Myanmar
3
Faculty of Animal Science, University of Mataram, Mataram,
Lombok, Indonesia

Corresponding author’s email: [email protected]

Abstract: The study aimed to compare the digestibility of different

pulse crop residues in goats. The research are to improve the
understanding of the nutritional composition and how to use
leguminous forages as ruminant feeds. The experiment was
conducted at the University of Veterinary Science, Myanmar. Green
Gram Residue (GGR), Groundnut Residue (GNR), Chickpea
Residue (CHR), Black Gram Residue (BGR) and Lablab Bean
Residue (LLBR) were used as experimental feed. A total of fifteen
male goats with 6-8 months old having 21±3 kg body weight were
randomly allocated into five groups with three goats per group in a
completely randomized design. Data were analysed with ANOVA
using SAS Software. The higher organic matter content was
observed in BGR and LLBR, while the crude protein content of
GNR and the fibre content of GGR were significantly higher
(p<0.05) than others. The dry matter, organic matter and acid
detergent fiber intake of goat fed BGR was highest and followed by
GNR. The significantly highest (p<0.05) fibre digestibility was
observed in goats fed LLBR while the lowest fiber digestibility was
found in goats fed BGR. The crude protein and digestible organic
matter intake of BGR was significantly higher (p<0.05) than those
of others.

Keywords: Goats, pulse crop residues, nutritive values, nutrient

intakes, digestibility of nutrient.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 149

Production Characteristics of Slaughter Cattle in Garut
Regency

G. T. Prilyadi1, H. Nuraini1, and R. Priyanto1

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Local cattle are the primary source of beef for the
industry in the Garut Regency. The regional beef supply typically
comes from the slaughter of these cattle at local abattoirs. This study
aimed to evaluate the productivity of slaughtered cattle and their
carcasses in Garut Regency. A total of 137 slaughtered cattle were
examined and categorized based on age, breed, sex, and body
condition score (BCS). The parameters measured included slaughter
weight, carcass weight, and carcass yield percentage. There were 7
breeds of local cattle, including Bali, Madura, Ongole Grade, FH
Grade, Limousin Grade, Simmental Grade, and Angus Grade were
identified among those slaughtered, with Limousin Grade (42%) and
Ongole Grade (34%) being the most common. Most cattle
slaughtered had I1-I3 dentition and BCS of 2-3. Notably, 74% of the
slaughtered cattle were female, while only 26% were male. The
slaughter weights and carcass yields varied significantly across the
different breeds. In general, the average slaughter weight, carcass
weight, and percentages of the local cattle slaughtered at the local
abattoir were 371.44 ± 93.54 kg, 162.69 ± 50.40 kg, and 43.42 ±
0.43%, respectively. Therefore, the cattle slaughtered in Garut
Regency exhibited suboptimal slaughter weights and lower carcass
productivity.
Keywords: Abattoir, Garut regency, local cattle, production
characteristics, slaughter cattle

150 | International Symposium on Innovative Approaches to Livestock Industry 2025

Zeolite/Copper Oxide Granules as Antibacterial Water Filter
to Prevent Pathogenic Bacterial Contamination in Laying
Hens Farm

Z. Abidin1, H. Salsabila1, Z. T. Maulitia1, P. N. Angelina1, and I.

I. Arief2
1
Department of Chemistry, Faculty of Mathematics and Natural
Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Chicken eggs are widely recognized as a cost-effective

and easily accessible source of protein in Indonesia. However, their
microbiological quality is poor due to contamination with
pathogenic bacteria, such as Escherichia coli. It is crucial to control
excess bacteria in the farm environment, especially in the water
consumed by laying hens. Although chlorine is commonly used as a
disinfectant in farm drinking water, it can interfere with chicken
metabolism, is not selective in killing microorganisms, and may
increase the resistance properties of microorganisms. To address this
issue, this study aims to develop zeolite granules modified with
copper oxide (CuO) to impart antibacterial properties. The granules
are produced by reacting zeolite and copper(II) nitrate solutions,
followed by a thermal process to decompose Cu 2+ into CuO. The
study has shown that the CuO-modified zeolite granules have
effective antibacterial abilities against E. coli and S. aureus bacteria,
with clear zone diameters of 6.97 ± 0.35 mm and 9.12 ± 0.23 mm,
respectively. This strongly suggests that the material possesses the
necessary qualities to be effectively employed as a reliable
antibacterial water filtration system specifically designed for
maintaining high water quality standards on a laying hens farm.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 151

 Keywords: antibacterial, composite, copper oxide, granule, laying
hens.

152 | International Symposium on Innovative Approaches to Livestock Industry 2025

Chromosome Assessment of Bali Cattle and Their Hybrids
Using the G-Banding Technique

Jakaria1, M. F. Ulum2, Sutikno3, and R. Priyanto1

1
Department of Animal Production Technology, Faculty of
Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Veterinary Medicine and Biomedicine College, IPB University,
Jl. Agatis Kampus IPB Dramaga, Bogor 16680, Indonesia
3
National Research and Innovation Agency, Cibinong, Bogor,
Indonesia

E-mail correspondence: [email protected]

Abstract. Chromosome determination is crucial, particularly in

identifying abnormalities in cattle. This study aims to determine
the chromosome number in Bali cattle and their hybrids using
the G- banding technique. A total of four Bali cattle and their
hybrids (Wagyu-Bali) (male and female) were analyzed.
Chromosome analysis was conducted in several stages: (1)
blood samples were collected with added heparin, (2) blood
cells were cultured using Karyo Max chromosome media
(Gibco), (3) incubation at 38°C, (4) after 70 hours, colchicine
was added and left for 2-3 hours, followed by centrifugation at
1000 rpm for 10 minutes and removal of the supernatant; the
cell pellet was then treated with a fixative solution, (5) staining
was performed using Giemsa stain, and (6) chromosome
observation and analysis (karyotyping) were carried out using
Cytovision software. The research found 60 (2n) chromosomes
in both Bali cattle and their hybrids. Additionally, the G-
banding technique allowed the determination of male and
female sexes. Therefore, it can be concluded that Bali cattle and
their hybrids (Wagyu-Bali) share the same chromosome
number.

Keywords: Bali cattle, Chromosome, Hybridization, Giemsa

staining, Karyotype.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 153

Blood Metabolites Profile, Physiological Response, and Estrus
Characteristics of Composite Sumatera Ewes Fed Flushing
Ration

L. Khotijah1, S. Munggaran1, Y. Adisti1, K. Komalasari1, D. M.

Fassah1, K. Komalasari1, A. Tarigan2, and D. A. Astuti1
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor,
Indonesia
2
Reserach Center for Animal Husbandry, National Research and
Innovation Agency, Bogor, Indonesia

E-mail correspondence: [email protected]

Abstract. This research aims to evaluate the effect of flushing ration

on nutrient intake, blood metabolites, physiological response, and
estrus of composite Sumatera ewes. This research used 10 composite
Sumatera ewes that had given birth once (average body weight 43.41
± 2.81 kg). The treatments were Sumatera Martabe Farm ration (P0)
and flushing ration (P1). The parameters observed were nutrient
intake, blood metabolites (glucose, triglyceride, and cholesterol) in the
pre-mating and post-mating phases, physiological response, and estrus
characteristics (onset of estrus, duration of estrus, and estrus
response). The data were analyzed using an independent sample t-test.
The results showed that the flushing ration significantly increased
(p<0.01) nutrient intake in both phases. The flushing ration
significantly increased (p<0.05) blood glucose at post-mating, and
increased (p<0.01) blood triglyceride and cholesterol levels during
post-mating phase. This also significantly accelerated (p<0.05) the
onset and duration of estrus. Flushing ration did not affect the
physiological responses of composite Sumatera ewes. It can be
concluded that the flushing ration increases nutrient intake in pre-
mating and post-mating phases, as well as blood metabolite levels in
post-mating phase, onset, and duration of estrus, reflecting adequate
nutritional status for reproduction.

154 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Keywords: blood metabolites, estrus, flushing, nutrient intake,
physiological response, reproduction

In Ovo Feeding of Pegagan Leaf (Centella asiatica) Extract

Improves Hatchability without Impairing Physical Quality of
Sentul Chicks

A. Darmawan1, R. Krisnan2, I. N. Fajri1, Y. Artika1, M. A.

Lusbiantoro1, I. Wijayanti1, R. K. Rusli3, and C. Hidayat2
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Indonesian Research Institute for Animal Production, Ciawi Bogor
16720, Indonesia
3
Department of Nutrition and Feed Technology, Faculty of Animal
Science, Universitas Andalas, 25175 Padang, Indonesia

E-mail correspondence: [email protected]

Abstract. This study aimed to assess the effectiveness of in-ovo

feeding of Centella asiatica leaf extract on the hatchability and
quality of Sentul chicks. A total of 300 fertile Sentul chicken eggs
with similar weight were randomly assigned to 5 treatments and 6
replicates (10 eggs per replicate). The treatments were uninjected
eggs (P0), eggs injected with 0.5 ml Phosphate Buffer Saline (PBS)
(P1), and eggs injected with 0.5 ml PBS containing 1 mg/egg (P2), 2
mg /egg(P3), and 4 mg/egg (P4) of Centella asiatica leaf extract. In-
ovo feeding of 4 mg/egg Centella asiatica leaf extract significantly
increased (p<0.05) hatchability but did not affect (p>0.05) hatching
weight, length of body chick, femur, tibia, and shank, and the score
of feather, eye, leg, and navel. It is concluded that in-ovo feeding of
Centella asiatica leaf extract up to 4 mg/egg increases hatchability
without adversely affecting the quality of Sentul chicks.

Keywords: hatchability, herbs, in-ovo, local chick.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 155

Production of Functional Forage: Gynura procumbens

N. R. Kumalasari1, W. S. Mulyati1, S. Lestari1, A. Rosalia1, and

A. U. Syahidah1
1
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Gynura procumbens is a shrub with significant potential

as an alternative livestock feed, known for its adaptability and
ability to thrive in various environments. This study aims to assess
the growth, biomass production, and phytochemical content of
Gynura procumbens, while identifying factors affecting its
performance. The research includes methods such as seedling
propagation, transplantation, and growth management. Results show
robust growth, high biomass yield, and the presence of beneficial
phytochemicals like flavonoids, saponins, and alkaloids, which
contribute to its nutritional value. Regular pruning and appropriate
fertilization enhance both leaf quality and growth rate. These
findings highlight Gynura procumbens as an eco-friendly,
phytochemical-rich feed option, offering sustainable solutions for
improving livestock feeding systems while reducing environmental
impact.

Keywords: growth, Gynura procumbens, production.

156 | International Symposium on Innovative Approaches to Livestock Industry 2025

Evaluation of Anti-Methanogenic Compound and Phytogenic
Extract of Moringa oleifera on Ruminal Methane
Production

M. S. Daulai1, I. Wijayanti2, Y. Retnani2, and S. Toshisada3

1
Graduate Student of Nutrition and Feed Science, Faculty of
Animal Science, IPB University, Bogor, Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Bogor, Indonesia
3
Department of Biological Molecular Chemistry, Faculty of
Agriculture, Kagawa University, Kagawa, Japan

E-mail correspondence: [email protected]

Abstract. Livestock is a significant contributor to methane

(CH4) emissions, a potent greenhouse gas. This research
investigates the potential of Moringa oleifera to reduce methane
emissions from ruminants. A comprehensive literature search
identified 24 relevant studies. The meta-analysis revealed that
M. oleifera supplementation significantly improves dry matter
digestibility (DMD) and organic matter digestibility (OMD)
while reducing methane emissions (p<0.01) without adversely
affected (p>0.05) ruminal fermentation parameters. Moringa
seeds had a significantly greater effect on methane reduction
compared to Moringa leaves and pods, by lowering acetate and
propionate ratio. Moringa seeds have been selected as a
promising source for the extraction and isolation process. Using
high-performance liquid chromatography (HPLC) and nuclear
magnetic resonance (NMR), 2,3,6-trihydroxybenzaldehyde was
isolated and identified from Moringa seeds. The in silico study
demonstrated that 2,3,6 trihydroxybenzaldehyde has a lower
binding affinity to the Methyl Coenzyme M Reductase (MCR)
enzyme compared to pterygospermin. Pterygospermin exhibited
the highest binding interaction with the enzyme's active site,
effectively inhibiting MCR. These findings suggest that M.
oleifera could serve as a phytogenic feed additive to mitigate
methane emissions.

Keywords: anti-methanogenic compound, methane, Moringa

oleifera, phytogenic extract, ruminal.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 157

Marinades Application to Reduce Malondialdehyde
Compound and Increase Antioxidant Activity in Fried
Chicken

Y. K. Silaban 1, T. Suryati1*, and Z. Wulandari1

1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Frying chicken at high temperatures can lead to the

oxidation of lipids and other components in the meat. Marinating
with ingredients high in antioxidants can inhibit the formation of
malondialdehyde (MDA), a toxic and mutagenic compound
produced during lipid oxidation reactions. This study aimed to
evaluate the ability of marinades to inhibit the formation of MDA
compounds and increase antioxidant activity in fried broiler
chickens. Broiler thighs were marinated using 4 different marinades
and a control seasoning (which included only salt and lime) for 12
hours. The marinated chicken was then fried in oil for 10 minutes,
with flipping occurring every 2 minutes during frying. The results
showed that marinades were significantly (p<0.05) able to inhibit
the MDA formation in fried chicken compared to the control,
increased the ability to inhibit free radical DPPH up to 74.69%, and
increased the antioxidant capacity up to 159.49 mg EVC g -1. The use
of marinades did not produce significant differences in
physicochemical characteristics, except that the color of the fried
chicken was a bit darker than the control. In conclusion, marinating
using different spice formulas increases antioxidant activity and
inhibits the formation of MDA in fried chicken.

Keywords: antioxidant, fried chicken, malondialdehyde,

marination, physicochemical properties.

158 | International Symposium on Innovative Approaches to Livestock Industry 2025

Association of Alcohol Dehydrogenase 1C Gene with Minerals
in Sheep

K. Listyarini, F. Muharram, and A. Gunawan

Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The Alcohol Dehydrogenase 1C (ADH1C) gene is

expressed at high levels in the liver and is predicted to play a role in
energy metabolism. The aim of this study was to identify
polymorphisms in the ADH1C gene and analyze its association with
minerals in sheep. A total of 85 Javanese Thin-Tailed rams, with an
average live weight of 25 kg at 12 months of age, were used to
identify polymorphisms and conduct association studies.
Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP) was employed to identify the single
nucleotide polymorphisms (SNPs) of the ADH1C gene. The
association of ADH1C genotypes with minerals was analyzed using
the General Linear Model. The SNP of the ADH1C gene was
polymorphic, with two genotypes identified: GG and CG. The Chi-
square test revealed that the ADH1C gene was in Hardy-Weinberg
equilibrium. The SNP of the ADH1C gene (g.25288858 C>G) was
significantly associated (P<0.05) with minerals, specifically
potassium (K). The CG genotype was associated with higher levels
of minerals, while the GG genotype exhibited the opposite effect.
Thus, the ADH1C gene (g.25288858 C>G) may contribute to
mineral levels in sheep.

Keywords: ADH1C, Minerals, Polymorphism, Sheep.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 159

The Body Size and Body Shape of Adult Pelung, Sentul,
Kampung, and IPB D1 Chickens

R. H. Mulyono, S. Darwati, C. Sumantri, and K. Listyarini

Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. The IPB D1 chicken is a composite breed consisting of

25% Pelung, 25% Sentul, 25% Kampung, and 25% Cobb broiler
chicken. Pelung, Sentul, and Kampung are native Indonesian breeds.
This study aimed to analyze the size and shape of adult Pelung,
Sentul, Kampung, and IPB D1 chickens. The analysis used eight
linear body measurements: femur length, tibia length, shank length,
shank circumference, third toe length, wing length, maxilla length,
and comb height, to identify size and shape through principal
component analysis. The first principal component (PC1) indicated
body size, while the second (PC2) indicated body shape. The
equations for PC1 and PC2 were established based on the measured
variables. Scatter plots revealed that Pelung chickens were the
largest, followed by IPB D1, Sentul, and Kampung chickens. Male
chickens consistently exhibited larger body sizes than females
across all breeds. While the body shape of IPB D1 overlapped with
that of the other breeds, distinct separations were noted among
Pelung, Sentul, and Kampung chickens, indicating variability in
body shape.

Keywords: Adult body shape, adult body size, IPB D1 chicken,

Kampung chicken, Pelung chicken, Sentul chicken.

160 | International Symposium on Innovative Approaches to Livestock Industry 2025

Evaluating the Efficacy of Panasonic WPS in Enhancing
Layer Chicken Performance

R. Adiyoga1*, I. I. Arief1, N. Ulupi1, Z. Abidin1, I. Komala1, and I.

G. Permana2
1
Department of Animal Production Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia
2
Department Nutrition and Feed Technology, Faculty of Animal
Science, IPB University, Jl. Agatis Kampus IPB Dramaga, Bogor
16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Poultry performance can be negatively affected by

microbial contamination in drinking water, leading to economic
losses in commercial chicken farming. This research aims to
evaluate the effect of Panasonic Water Purification System (WPS)-
filtered water on the productivity of ISA Brown laying hens
compared to unfiltered water. The study was conducted over 30
days with 2,000 hens aged 57 weeks. Water samples were taken
from the water tank and drinking nipples for both treatments (WPS
and No WPS), and production performance data—including
mortality, hen-day production, total egg production, egg mass, and
feed conversion ratio (FCR)—was recorded daily. Results revealed
a significant reduction in pathogenic microbes in the WPS-treated
water, peaking on day 15 and stabilizing thereafter. After 30 days,
WPS-treated hens showed a 66.6% decrease in mortality, a 2.08%
increase in hen-day production, a 1.4 kg rise in total egg production,
a 0.5 decrease in FCR, and a 0.25 g/egg reduction in egg mass.
These findings highlight the importance of clean water in improving
the productivity and health of layer chickens, offering potential
economic benefits to commercial farms.

Keywords: Egg production, Layer chicken, Pathogen, Poultry

management, Water quality.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 161

162 | International Symposium on Innovative Approaches to Livestock Industry 2025
Analysis of Human Resources in Tajur Halang Village Dairy
Farm for Agrotourism Development

M. A. Nabil, L. Cyrilla, I. Komala*, and Yumita

Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia

E-mail correspondence: [email protected]

Abstract. Dairy farming in Tajur Halang Village has good prospects

for being developed into agrotourism. Therefore, data on competent
human resources in animal husbandry is needed to support
agrotourism. This research aims to analyze human resources on
dairy farms in Tajur Halang Village for the development of
agrotourism. Data was obtained by conducting interviews using a
Likert scale related to several aspects, namely farmer’s competence,
attractions, membership and social culture. Interviews were
conducted with 24 respondents from the Mandiri Sejahtera
Livestock Group. The results of the research showed that the
analysis of human resources in sequence is membership (4.32) very
good, socio-cultural (4.31) very good, attractions (4.15) good, and
farmer’s competence (3.70) good. Every aspect needs to be
maintained and improved to support the development of agrotourism
in Tajur Halang Village.

Keywords: agrotourism, categories, human resource, livestock

group.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 163

Smart Microclimate Control System using Internet of Things
(IoT) on Physiological Responses of the Dairy Cow

I. Komala1,*, M. Fayruz2, A. Yani2, M. T. Mubarrok2, and Y. B.

Gampur2
1
Department of Animal Production and Technology, Faculty of
Animal Science, IPB University, Jl. Agatis Kampus IPB Dramaga,
Bogor 16680, Indonesia
2
Study Program Management, Sekolah Tinggi Ilmu Ekonomi (STIE)
Ganesha, Jakarta, Indonesia
E-mail correspondence: [email protected]

Abstract. This study aimed to evaluate the physiological responses of

the dairy cows used smart microclimate control system (SMCS) D-
Ruminansia in dairy cow base on Internet of Things (IoT). This
research used ten lactation cows at Balai Embrio Ternak Cipelang and
ten lactation cows at Kelompok Ternak Mandiri Sejahtera, 500-600 kg
of body weight and 2.7-3.2 of body condition score were used in this
study. They were housed in stanchion barn with cement floors, fed
twice daily in the form of total mixed ration (TMR) and given ad
libitum amounts of drinking water. Ambient temperature, relative
humidity and temperature humidity index (THI) were recorded real
time at 24 hours. Physiological responses i.e. rectal temperature (RT),
heart rate (HR) and respiration rate (RR) and heat tolerance coefficient
(HTC) were also recorded. Descriptive statistics such as mean and
standard deviation were performed using SPSS software. The results
showed that both ambient temperature and THI reached their
maximum (temperature 25.14±1.12oC at 0.1 PM, humidity 80.64±5.52
at 06.30 AM and THI 74.45±1.69 at 01.00 PM, respectively) and
minimum (temperature 19.65±1.30oC at 04.00 AM, humidity
74.23±6.87 at 01.00 PM, and THI 66.33±0.90 at 04.00 AM,
respectively). The average ambient temperature was 22.00+2.65 oC,
which was in accordance with the thermo neutral zone (TNZ: 16-
25oC) for dairy cows, with the average humidity was 78.93+3.14. The
physiological responses (RT, PR and RR) and HTC increased in line
with the increasing ambient temperature and THI. The dairy cows in
general are exposed feel comfortable, only at 01.00 PM dairy cow

164 | International Symposium on Innovative Approaches to Livestock Industry 2025

 experience mild stress due to increasing ambient temperature, relative
humidity and THI. Generally, the cows show the ability to adapt heat
stress by balancing their body temperature, pulse rate and respiration
rate.

Keywords: heat stress, humidity, temperature, temperature humidity

index.

International Symposium on Innovative Approaches to Livestock Industry 2025 | 165

 List of ICARE Committee

Honorable Adviser: Dean of Faculty of Animal Science, Bogor

Agricultural University (Dr. Ir. Idat Galih Permana,
MSc.Agr.)

Steering Committee: Dr. Ir. Idat Galih Permana, M.Sc.Agr

Dr. Sri Suharti, S.Pt., M.Si
Prof. Dr. Irma Isnafia Arief, S.Pt, M.Si
Prof. Dr. Ir. Muladno, MSA
Prof. Dr. Ir. Komang G. Wiryawan
Prof. Dr. Asnath M. Fuah, MS
Prof. Dr. Despal, S.Pt., M.Sc.Agr
Prof. Dr. Agr. Asep Gunawan, M.Sc
Himpunan Ilmuwan Peternakan Indonesia (HILPI)

Scientific Committee & Proceeding:

Chairman: Dr. Tuti Suryati, S.Pt., M.Si
Members: Prof. Ermias Kebreab
Prof. Kazuhito Fujiyama
Prof. Satoshi Koike
Prof. Myunggi Baik
Dr. Muhammad Jasim Uddin
Dr. Ir. MA (Maja) Slingerland
Prof. Dr. Anuraga Jayanegara, S.Pt., M.Sc
Prof. Dr. Irma Isnafia Arief, SPt., M.Si
Prof. Dr. Ir. Iman Rahayu Hidayati, MS
Prof. Dr. Ir. Toto Toharmat, M.Agr. Sc
Prof. Dr. Ir. Ronny Rachman Noor, M.Rur.Sc
Prof. Dr. Ir. Cece Sumantri, M.Agr.Sc
Prof. Dr. Ir. Nahrowi, MSc.
Prof. Dr. Ir. Muladno, MSA
Prof. Dr. Ir. Komang G. Wiryawan
Prof. Dr. Ir. Luki Abdullah
Prof. Dr. Ir. Dewi Apri Astuti, MS
Prof. Dr. Ir. Erika B. Laconi, MS
Prof. Dr. Ir. Sumiati, M.Sc
Prof. Dr. Ir. Panca Dewi MHK, MS
Prof. Dr. Ir. Yuli Retnani, M.Sc
Prof. Dr. Ir. Asnath Maria Fuah, MS
Prof. Dr. Ir. Niken Ulupi, MS
Prof. Dr. Ir. Asep Sudarman, M.Rur.Sc
Prof. Dr. agr. Asep Gunawan, S.Pt. M.Sc

166 | International Symposium on Innovative Approaches to Livestock Industry 2025

 Prof. Dr. Ir. Rudy Priyanto
Prof. Dr. Jakaria, SPt., MSi
Prof. Dr. Despal, SPt., M.Sc.Agr
Prof. Dr. Syahruddin Said
Dr. Cahyo Budiman, S.Pt., M.Sc
Dr. Epi Taufik, S.Pt., MVPH, MSi.
Dr. Ir. Muhammad Ridla, M.agr.
Dr. Yantyati Widyastuti
Dr. Wulansih Dwi Astuti
Dr. Roni Ridwan

Organizing Committee:
Chairman: Prof. Dr. Ir. Jakaria, S.Pt., M.Si
Vice Chairman: Dr. Windi Al Zahra, S.Pt., M.Si
Dr. rer. Nat. Nur Rochmah Kumalasari, S.Pt., M.Si
Secretary: Dr. Dilla Mareistia Fassah, S.Pt., M.Sc
Dr. Isyana Khaerunnisa, S.Pt. M.Si
Secretariat: Irma Nuranthy Purnama, S.Pt., MSi
Indriani, S.Pt
Irmayanti
Treasurer: Dr. Zakiah Wulandari, S.TP. M.Si
Dr. Ir. Lilis Khotijah, M.Si
Seminar Program: Dr. Sigid Prabowo, S.Pt., M.Sc
Dr. Epi Taufik, S.Pt., MVPH, Msi
M. Baihaqi, S.Pt., M.Sc
Arif Darmawan, S.Pt., M.Sc
Dr. Indah Wijayanti, S.TP., M.Si
Dr. Yuni Cahya Endrawati, S.Pt., M.Si
Dr. Iyep Komala, S.Pt., M.
IT Program: M. Arifin, S.Pt., M.Si
Sazli Tutur Risyahadi, S.TP., M.T
Sugeng Tri Wahyono
Femmy
Logistic: Dr. M. Sriduresta, S.Pt., M.Sc
Verika Armansyah Mendrova, S.Pt., M.Si
Edit Lesa Aditia, S.Pt., M.Sc
Mad Haris
Fund Raising & Dr. Astari Apriantini, S.Gz., M.Sc
Finance: Vivin Hadiningrum, SPt
Refreshment: Dr. Ir. Widya Hermana, M.Si
Dr. Ir. Sri Darwati, M.Si
Triyati

International Symposium on Innovative Approaches to Livestock Industry 2025 | 167