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Protein Blood

The document outlines various tests for detecting abnormal constituents in urine, including tests for proteins, blood, reducing sugars, ketone bodies, bile salts, and bile pigments. Each test is described with its procedure, observation, and inference regarding the presence or absence of specific substances. The final result indicates the presence of proteins and blood in the urine sample tested.

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3 views2 pages

Protein Blood

The document outlines various tests for detecting abnormal constituents in urine, including tests for proteins, blood, reducing sugars, ketone bodies, bile salts, and bile pigments. Each test is described with its procedure, observation, and inference regarding the presence or absence of specific substances. The final result indicates the presence of proteins and blood in the urine sample tested.

Uploaded by

dhiva1103har
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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DETECTION OF ABNORMAL CONSTITUENTS IN URINE

Experiment Observation Inference


1. Heat coagulation test:
Fill 3/4th of the test tube with urine. Heat A white coagulum is Presence of Proteins
the upper part of the test tube to boil. formed, which does
not dissolve on adding
Now add about 10 drops of 1% acetic acid, 1% acetic acid
drop by drop.

2. Sulphosalicylic acid test: A white precipitate is Presence of Proteins


formed
To 5 ml of urine add few drops of
25% sulpho salicylic acid solution. Mix well.

3. Heller’s test:
A white ring is formed Presence of Proteins
To 2 ml of urine, add 2 ml of at the junction of two
con. Nitric acid along the sides of the liquids
test tube.

4. Benzidine test for blood in urine:


A black coloured Presence of blood
Take a knife-point of Benzidine in a clean
precipitate is formed
test tube and add 1 m of glacial acetic acid.
Shake well till the powder is dissolved and
add 1 ml of hydrogen peroxide.
Now add 1 ml of urine.
5. Benedict's test for reducing sugars:
No characteristic Absence of Reducing
To 5 ml of Benedict's solution in a test tube, change in colour sugars
add exactly 8 drops of urine. of the solution
Mix well and heat the solution till it begins
to boil.
DETECTION OF ABNORMAL CONSTITUENTS IN URINE
Experiment Observation Inference

6. Rothera's test:
Saturate 5 ml of urine with solid Absence of ketone
No violet colour ring is bodies
ammonium sulphate. Add 5 drops of
formed
freshly prepared 5% sodium nitroprusside
solution and shake gently. Add 1 to 2 ml
of strong ammonia along the sides of the
test tube.
7. Hay's test for bile salts:
Add 5 ml of the given sample of urine to a
test tube marked 'urine' and 5 ml of water Sulphur powder does Absence of bile salts
to another test tube marked 'control'. not sink in both the test
Sprinkle a spatula full of finely powdered tubes
sulphur on the fluid surface in each test
tube.

8. Fouchet's test:
Take 5 ml of urine in a test tube add few
crystals of magnesium sulphate and mix
the contents till it dissolves. Now add 2 ml No green-coloured Absence of Bile
precipitate is formed pigments
of 10% BaCl2.
A precipitate of BaSO4 is formed. Allow
the tube to stand for 10 minutes and then
filter the contents of the tube on a filter
paper.Discard the filtrate. Open out the
filter paper and allow it to dry.
Add a drop or two of Fouchet's reagent to
the precipitate on the filter paper.

Result

The abnormal constituents present in the given urine sample are Protein and blood.

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