Certificate of Analysis – Certified Reference Material
Certipur® Buffer solution pH 4.00 (20°C)
Certified Reference Material for pH measurement
Product no.: 1.09435.1000
Lot no.: HC15176435
Description of CRM: Certipur® Buffer solution pH 4.00 (20°C)
Certified Reference Material for pH m easurement
Expiry date: 2024/02/29
Storage: +15°C to +25°C tightly closed in the original container
Composition: citric acid / sodium hydroxide / hydrogen chloride
Associated uncertainty, U=k .u
Certified value
(k=2)
pH value 4.01 ± 0.02 (20°C)
Metrological traceability: The pH value of this certified buffer solution is directly traceable to prim ary
certified reference m aterials characterised by PTB and verified by SRMs from
NIST.
NIST 189c, 188, 185i, 186 Ig, 186 IIg, 187f
PTB OX-405/18, TA-442/19, PHT-467/20, PHO-490/20, BO-468/20
PTB: Physikalisch Technische Bundesanstalt, Braunschweig, Germany
NIST: National Institute of Standards and Technology, Gaithersburg, USA.
Measurement method: pH value is m easured with a com bined glass electrode after 5-point calibration
according to DIN 19268 with reference buffer solutions according to DIN
19266, IUPAC, NIST, Ph.Eur. and USP.
Accreditation: Merck KGaA, Darm stadt, Germ any is accredited by the Germ an accreditation
authority DAkkS as registered reference m aterial producer D-RM-15185-01-00
in accordance with ISO 17034.
Certificate issue date: 2021/02/10
CRM released by Approving Officer
or delegate LS-OII-QS3
ISO 17034
Dipl.-Ing. Ayfer Yildirim
Responsible Manager of LS-OII-QS3
(Accredited Calibration Laboratory)
Merck KGaA, 64271 Darmstadt, Germany, Tel.: +49 (0)6151 72-24 40
EMD Millipore Corporation, 400 Summit Drive Burlington MA 01803, USA, Tel. +1-978-715-4321
Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd., 2149 Winston Park, Dr. Oakville,
Ontario, L6H 6J8, Phone: +1 800-565-1400
Certificate Page 1 of 2 Certificate version 01
Intended use: This reference m aterial is intended for use as a calibration standard for pH
instrum ents or pH electrodes or as a control sam ple for m easuring the pH value.
Instructions for handling The pH value is strongly dependent on the temperature. It is therefore necessary
and correct use: to keep the tem perature constant within the m easurement.
Health and safety Please refer to the Safety Data Sheet for detailed inform ation about the nature of
information: any hazard and appropriate precautions to be taken.
Preparation: This reference m aterial is prepared gravim etrically from citric acid, sodium
hydroxide, hydrogen chloride and high purity water.
Associated uncertainty:
The expanded uncertainty UCRM is calculated as UCRM = k·u CRM, where k = 2 is the coverage factor for a 95%
coverage probability and u CRM is the com bined standard uncertainty in accordance to ISO 17034.
The com bined uncertainty u CRM is derived from combination of the squared uncertainty contributions:
𝑢𝐶𝑅𝑀 = √𝑢 2𝐶ℎ𝑎𝑟𝑎𝑐𝑡𝑒𝑟𝑖𝑠𝑎𝑡𝑖𝑜𝑛 + 𝑢 2𝐻𝑜𝑚𝑜𝑔𝑒𝑛𝑒𝑖𝑡𝑦 + 𝑢 2𝑆𝑡𝑎𝑏𝑖𝑙𝑖𝑡𝑦
u characterisation : is the uncertainty in accordance with DIN EN ISO/IEC 17025 which includes the
contributions of the prim ary reference m aterial and the m easuring system. The
characterisation measurements have been conducted by our DAkkS accredited
calibration laboratory (D-K-15185-01).
u homogeneity: is the between-bottle variation in accordance with ISO 17034. The assessment
of hom ogeneity is perform ed by analysis of a representative num ber of
systematically chosen sample units.
u stability: is the uncertainty obtained from short-term and long-term stability in accordance
with ISO 17034. The stability studies are the basis for the quantification of the
expiry date of this reference m aterial for the unopened bottle .
Informative values:
Tem perature dependence 1: Tem perature [°C] ∆ pH
0 + 0.05
5 + 0.04
10 + 0.02
15 + 0.01
20 ±0
25 + 0.01
30 + 0.01
35 + 0.01
40 + 0.01
50 ±0
1
Tem perature deviation data provided for reference only. Values are not batch -specific and should not be considered
certified values.
For more detailed information please read the certification report on our website.
Certificate of analysis revision history:
Certificate version Date Reason for version
01 2021/02/10 Initial version
The vibrant M, Supelco, Certipur and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates.
Detailed information on trademarks is available via publicly accessible resources.
© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
Certificate Page 2 of 2 Certificate version 01
