INSTITUTE OF PHARMACEUTICAL SCIENCES
UNIVERSITY OF LUCKNOW
Lecture Plan B. Pharm Session 2025-26 Semester/Year: 3rdSem/ 2nd Year
Subject Name: Pharmaceutical Microbiology Theory Subject code: BP303T
Faculty Name: Dr. Namrata Singh
Lectur Title of Lecture Unit Teaching Method Reference
e No. (PPT/ Whiteboard) (Books/Journals/Others)
1 Introduction, history of 1 Whiteboard/PPT 1. W.B. Hugo and
Microbiology A.D. Russel:
2 its branches, scope and its 1 Whiteboard/PPT Pharmaceutical
importance Microbiology,
3 Introduction to Prokaryotes 1 Whiteboard/PPT Blackwell
and Eukaryotes, Study of Scientific
ultra-structure and publications,
4 morphological classification of 1 Whiteboard/PPT Oxford London.
bacteria 2. I.P., B.P., U.S.P.-
5 nutritional requirements, raw 1 Whiteboard/PPT latest editions
materials used for culture 3. Prescott and
media and physical parameters Dunn., Industrial
for growth, growth curve Microbiology, 4th
edition, CBS
Publishers &
6 isolation and preservation 1 Whiteboard/PPT Distributors,
methods for pure cultures Delhi.
4. Pelczar, Chan
7 cultivation of anaerobes, 1 Whiteboard/PPT Kreig,
quantitative measurement of Microbiology,
bacterial growth (total & Tata McGraw Hill
viable count) edition
5. N.K. Jain:
Pharmaceutical
8 Study of different types of 1 Whiteboard/PPT
phase contrast microscopy, Microbiology,
dark field microscopy and Vallabh
electron microscopy Prakashan, Delhi
9 Identification of bacteria using 2 Whiteboard/PPT 6. Ravi Kumar
staining techniques (simple, Maddali,
Gram’s &Acid-fast staining) Pharmaceutical
and biochemical tests (IMViC)
Microbiology, 2nd
10 Study of principle, procedure, 2 Whiteboard/PPT edition, Pharma
merits, demerits of med press,
sterilization Hydrabad
11 3 Whiteboard/PPT 7. Cooper and
applications of physical, Gunn’s, Tutorial
chemical gaseous, radiation Pharmacy, CBS
and mechanical method of Publishers and
sterilization
Distributors, Delhi
12 Evaluation of the efficiency of 3 Whiteboard/PPT
sterilization methods 8. Tortora, Funke,
13 Equipments employed in large 3 Whiteboard/PPT and Case's
scale sterilization Microbiology, An
14 Sterility Indicators 3 Whiteboard/PPT Introduction, 13
15 Study of morphology, 3 Whiteboard/PPT Edition
classification, of fungi and
viruses.
16 Reproduction/replication of 3 Whiteboard/PPT
fungi and viruses
17 and cultivation of fungi and 3 Whiteboard/PPT
Viruses
18 Classification and mode of 3 Whiteboard/PPT
action of disinfectants
19 Factors influencing 3 Whiteboard/PPT
disinfection, antiseptics and
their evaluation
20 For bacteriostatic and 3 Whiteboard/PPT
bactericidal actions
21 Evaluation of bactericidal & 3 Whiteboard/PPT
Bacteriostatic
22 Sterility testing of products 3 Whiteboard/PPT
(solids, liquids, ophthalmic
and other sterile products)
according to IP,
23 Sterility testing of products 3 Whiteboard/PPT
(solids, liquids, ophthalmic
and other sterile products)
according to BP
24 Sterility testing of products 3 Whiteboard/PPT
(solids, liquids, ophthalmic
and other sterile products)
according to USP
25 Designing of aseptic area 4 Whiteboard/PPT
26 Laminar flow equipments 4 Whiteboard/PPT
27 study of different sources of 4 Whiteboard/PPT
contamination in an aseptic
area and methods of
prevention
28 clean area classification 4 Whiteboard/PPT
29 Principles and methods of 4 Whiteboard/PPT
different microbiological assay
30 Methods for standardization of 4 Whiteboard/PPT
antibiotics
31 Methods for standardization of 4 Whiteboard/PPT
vitamins
32 Methods for standardization of 4 Whiteboard/PPT
amino acids
33 Assessment of a new antibiotic 4 Whiteboard/PPT
34 Types of spoilage 5 Whiteboard/PPT
35 factors affecting the microbial 5 Whiteboard/PPT
spoilage of pharmaceutical
products
36 sources and types of microbial 5 Whiteboard/PPT
contaminants, assessment of
microbial contamination and
spoilage
37 Preservation of pharmaceutical 5 Whiteboard/PPT
products using antimicrobial
agents,
38 evaluation of microbial 5 Whiteboard/PPT
stability of formulations
39 Growth of animal cells in 5 Whiteboard/PPT
culture
40 general procedure for cell 5 Whiteboard/PPT
culture,
41 Primary, established and 5 Whiteboard/PPT
transformed cell cultures
42 Application of cell cultures in 5 Whiteboard/PPT
pharmaceutical industry and
research.
