INFRARED SPECTROSCOPY: Outline of Presentation

• Introduction
• Units & measurement
• Range of IR radiation
• Principle
• Theory- Molecular vibrations
• Vibrational Frequency- Hook’s Law
• Number of Fundamental vibrations
• Factors affecting vibrational Frequency
• Instrumentation
• Sampling Techniques
• IR Spectrum-Examples
• Advantages & disadvantages
• Applications
Wide Range of Types of Electromagnetic Radiation in nature.
1. Only a small fraction (350-780 nM is visible light).
2. The complete variety of electromagnetic radiation is used throughout
spectroscopy.
3. Different energies allow monitoring of different types of interactions with
matter.
E=hn = hc/l
SPECTROSCOPY - Study of spectral information

Physical response Detecting

Molecule
stimulus instrument

Visual (most common)

representation, or
Spectrum

Upon irradiation with infrared light, certain bonds respond by

vibrating faster. This response can be detected and translated
into a visual representation called a spectrum.
EFFECT OF ELECTROMAGNETIC RADIATION
ON MOLECULES

Graphics source: Wade, Jr., L.G. Organic Chemistry, 5th ed. Pearson Education Inc., 2003
 The unit used on an IR spectrum is
Wavenumbers n

n = wavenumbers (cm-1) = 1
l
wavelength (cm)

n = frequency = nC C = speed of light

= 3 x 1010 cm/sec
or

1 C
n =( ) C = cm/sec
=
1
l l cm sec

wavenumbers are directly proportional to frequency 6

 Range of IR
• Near IR: 0.8 to 2.5m (12000cm-1 –4000cm-1)
• Analyzing mixtures of aromatic amines
• Determination of protein, fat, moisture, oil content.

• Middle IR: 2.5 to 15m ( 4000cm-1 – 667cm-1)

• Also known as vibration- rotation region.
• This region is divided into:
1. Group frequency region: 4000cm-1 – 1500cm-1
2. Fingerprint region: 1500cm-1 – 667cm-1

• Far IR: 15 to 1000m (667cm-1 –10cm-1 )

• Study of inorganic or organometallic compounds
• Sensitive to changes in overall structure of the molecule
 MOLECULES



ATOMS



CONTINOUS MOTION
OR
NATURAL VIBRATIONS



APPLIED IR FREQUENCY = NATURAL FREQUENCY

ABSORPTION OR IR

VIBRATIONS AT MANY RATES


IR SCPECTRA

CHARACTERISTIC FUNCTIONAL GROUPS &
BONDS ( FINGERPRINT OF A MOLECULE)
Principle – IR Spectroscopy
 Infrared radiation is largely thermal energy.
It induces stronger molecular vibrations in covalent bonds, which can be
viewed as springs holding together two masses, or atoms.

Specific bonds respond to (absorb) specific frequencies

Graphics source: Wade, Jr., L.G. Organic Chemistry, 5th ed. Pearson Education Inc., 2003
 Number of Vibrational Modes:
Decided by no. of Vibrational degrees of Freedom (DOF)
Every atom in a molecule has 3 DOF in three Cartesian
coordinates which defines its position in space.
3n= Vibrational DOF + Translational DOF +Rotational DOF
(where n=No. of atoms)
We have to calculate Vibrational DOF
• for linear molecules, number of types of vibrations:
3n= Vibrational DOF + Translational DOF (3) +Rotational DOF (2)
Vibrational DOF= 3n-3-2 =3n-5
•for non-linear molecules, number of types of vibrations: 3n=
Vibrational DOF + Translational DOF (3) +Rotational DOF (3)
Vibrational DOF= 3n-3-3 =3n-6
Examples:
1) HCl: 3(2)-5 = 1 mode

2) CO2: 3(3)-5 = 4 modes

- + -
moving in-out of plane

3) C6H6: 3n-6=3(12)-6=30

•why so many peaks in IR spectra?

- Overtones (2x, 2y), Combination (x+y. x+2y, 2x+y) and
Difference bands (x-y. 2x-y, 2y-x etc.): 10-100 times less intense than
fundamental
• observed vibration can be less then predicted because
• symmetry ( no change in dipole)
• energies of vibration are identical
• absorption intensity too low
• frequency beyond range of instrument
IR Active Vibrations:
- In order for molecule to absorb IR radiation:
• vibration at same frequency as in light
• but also, must have a change in its net dipole moment
as a result of the vibration
Examples:
1) CO2: 3(3)-5 = 4 modes  = 0; IR inactive
d- 2d+ d-
 > 0; IR active
d- 2d+ d-
- + -  > 0; IR active
d- 2d+ d-
degenerate –identical energy single IR peak
2d+
 > 0; IR active
d- d-
 TRANSMISSION vs. ABSORPTION
When a chemical sample is exposed to the action of IR LIGHT, it can absorb
some frequencies and transmit the rest. Some of the light can also be
reflected back to the source.

Transmitted light
IR Chemical
Detector
source sample

From all the frequencies it receives, the chemical sample can

absorb (retain) specific frequencies and allow the rest to pass
through it (transmitted light).

The detector detects the transmitted frequencies, and by doing so also reveals the values of
the absorbed frequencies.
 AN IR SPECTRUM IN ABSORPTION MODE
The IR spectrum is basically a plot of transmitted (or absorbed) frequencies vs.
intensity of the transmission (or absorption). Frequencies appear in the x-axis in
units of inverse centimeters (wavenumbers), and intensities are plotted on the y-
axis in percentage units.

The graph above shows a spectrum in absorption mode.

 AN IR SPECTRUM IN TRANSMISSION MODE

The graph above shows a spectrum in transmission mode.

This is the most commonly used representation and the one found in most chemistry
and spectroscopy books. Therefore we will use this representation.
 CLASSIFICATION OF IR BANDS
IR bands can be classified as strong (s), medium (m), or weak (w), depending on
their relative intensities in the infrared spectrum. A strong band covers most of
the y-axis. A medium band falls to about half of the y-axis, and a weak band falls
to about one third or less of the y-axis.
 INFRARED ACTIVE BONDS
Not all covalent bonds display bands in the IR spectrum. Only polar bonds do
so. These are referred to as IR active.
The intensity of the bands depends on the magnitude of the dipole moment
associated with the bond in question:
• Strongly polar bonds such as carbonyl groups (C=O) produce strong bands.
• Medium polarity bonds and asymmetric bonds produce medium bands.
• Weakly polar bond and symmetric bonds produce weak or non observable
bands.
 INFRARED BAND SHAPES
Infrared band shapes come in various forms. Two of the most common are
narrow and broad. Narrow bands are thin and pointed, like a dagger. Broad
bands are wide and smoother.

A typical example of a broad band is that displayed by O-H bonds, such as

those found in alcohols and carboxylic acids, as shown below.
v Str. Free O-H > v Str. Intermol. H bonded O-H >v Str. Intramol. H bonded O-H
IR Spectrophotometer: Advantages
1. All kinds of material can be analyzed.
2. Provides lot of information.
3. Fast and easy.
4. Very small amount of sample is required.
5. Less expensive.

Disadvantages
1. IR spectra cannot be obtained for Mono atomic substances e.g.: helium, argon.
2. IR spectra cannot be obtained for Homonuclear diatomic molecules like O2 , N2 .
3. Works best for pure substances but not for complex mixtures.
 INFORMATION OBTAINED FROM IR SPECTRA

• IR is most useful in providing information about the presence or absence

of specific functional groups.

• IR can provide a molecular fingerprint that can be used when comparing

samples. If two pure samples display the same IR spectrum it can be
argued that they are the same compound.

• IR does not provide detailed information or proof of molecular formula or

structure. It provides information on molecular fragments, specifically
functional groups.

• Therefore it is very limited in scope, and must be used in conjunction with

other techniques to provide a more complete picture of the molecular
structure.
 IR Absorption Regions
In general, the IR spectrum can be split into four regions for
interpretation:

 4000  2500 cm-1: Absorption of single bonds formed by hydrogen

and other elements e.g. OH, NH, CH

 2500  2000 cm-1: Absorption of triple bonds e.g. C≡C, C≡N

 2000  1500 cm-1: Absorption of double bonds e.g. C=C, C=O

 1500  400 cm-1: This region often consists of many different,

complicated bands, called the fingerprint region.
It is rarely used for identification of particular
53
functional groups
 Summary of IR Absorptions

Graphics source: Wade, Jr., L.G. Organic Chemistry, 5th ed. Pearson Education Inc., 2003
Typical IR Absorption Regions

55
 Examples: IR Spectrum of Alcohols

Focus your analysis on this region. This is where most stretching Fingerprint region: complex and difficult to
frequencies appear. interpret reliably.

Graphics source: Wade, Jr., L.G. Organic Chemistry, 6th ed. Pearson Prentice Hall Inc., 2006