Biotechnology 5.
PCR uses the enzyme:
MCQs A) Lactase
1. Joining DNA from two different species is called: B) Amylase
A) Transformation C) Taq polymerase
B) Recombinant DNA D) Reverse transcriptase
C) Cloning
6. Suspension culture can be used to produce which
D) Transduction plant metabolite?
A) Morphine
2. Which enzyme cuts DNA at specific sites?
B) Quinine
A) Taq polymerase
C) Penicillin
B) Ligase
D) Insulin
C) Restriction endonuclease
D) Telomerase 7. Which is a thermostable enzyme used in PCR?
A) Pepsin
3. The process of amplifying DNA in vitro using repeated
B) Trypsin
cycles of heating and cooling is called:
C) Taq polymerase
A) Cloning
D) DNAase
B) Northern blotting
C) Polymerase Chain Reaction (PCR)
8. Plasmids most commonly used as cloning vectors in E.
D) Translation coli are derived from:
A) pBR322
4. Enzyme required to join Okazaki fragments during
B) Lambda phage
DNA replication:
C) ColE1
A) DNA polymerase
D) F plasmid
B) DNA ligase
C) Helicase
9. Gene synthesis from mRNA uses which enzyme?
D) RNA primase
A) RNA polymerase
B) Reverse transcriptase
C) DNA polymerase I
D) Ligase
10. In cystic fibrosis, the defective protein is: 15. The enzyme that separates DNA strands in PCR is:
A) Albumin A) Helicase
B) CFTR B) Heat
C) Hemoglobin C) Ligase
D) G6PD D) Isomerase
11. DNA fragments can be separated by: 16. Which is an example of a vector commonly used in
genetic engineering?
A) Centrifugation
A) YAC
B) Gel electrophoresis
B) Lambda phage
C) Chromatography
C) Fungi
D) Dialysis
D) Prion
12. Genetically engineered bacteria for insulin
production often contain a gene for resistance to: 17. The absence of adenosine deaminase (ADA) causes:
A) Penicillin A) Thalassemia
B) Ampicillin B) Down syndrome
C) Vancomycin C) SCID
D) Streptomycin D) Hemophilia
13. Plants carrying foreign genes are called: 18. The first successful gene therapy was for:
A) Hybrids A) Hemophilia
B) Polyploids B) Cystic fibrosis
C) Transgenic plants C) SCID
D) Mutants D) Sickle cell anemia
14. What is the product when DNA from two different 19. In biotechnology, what is a probe?
cells is combined?
A) Monoclonal antibody
A) cDNA
B) Labeled DNA/RNA fragment
B) Chimeric DNA
C) PCR product
C) Plasmid
D) Restriction enzyme
D) Vector
20. Complementary DNA (cDNA) is synthesized from: 25. Use of living organisms for producing useful
products is:
A) mRNA
A) Transgenics
B) tRNA
B) Biotechnology
C) genomic DNA
C) Immunology
D) rRNA
D) Cytogenetics
21. The main function of molecular scissors in
biotechnology is to: 26. Restriction endonucleases naturally occur in:
A) Join DNA fragments A) Humans
B) Cut DNA at specific sites B) Bacteria
C) Amplify DNA C) Virus
D) Ligate DNA D) Plants
22. Transgenic animals are animals that: 27. The role of monoclonal antibodies is to:
A) Undergo asexual reproduction A) Destroy red blood cells
B) Have foreign DNA inserted B) Bind to specific antigens
C) Are cloned C) Synthesize proteins
D) Have mutated mitochondria D) Carry oxygen
23. In SCID, the defect is usually due to the lack of: 28. The discovery of restriction enzymes was essential
for:
A) B cells
A) Monoclonal antibody production
B) ADA enzyme
B) Genetic engineering
C) Insulin
C) DNA replication
D) Hemoglobin
D) Protein synthesis
24. Common effect of cystic fibrosis is on:
29. Gene therapy for cystic fibrosis aims to:
A) Muscle cells
A) Replace mutated CFTR gene
B) Epithelial cells
B) Remove lungs
C) Cardiac cells
C) Inhibit beta cell destruction
D) Lymphocytes
D) Insert mutated hemoglobin gene
30. The first genetically engineered "medicine"
produced by bacteria was:
A) Antibiotic
B) Human insulin
C) Hepatitis vaccine
D) Hemoglobin
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