Soyabean Casein Digest Agar Plate w/ 5% Sheep blood MP5211
Intended use:
A general purpose medium used for the cultivation of wide variety of microorganisms and to study the haemolytic reactions.
Composition**
Ingredients Gms / Litre
Tryptone # 15.000
Soya peptone 5.000
Sodium chloride 5.000
Agar 15.000
Sheep blood 50 ml
Final pH ( at 25°C) 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Pancreatic digest of casein
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Soyabean Casein Digest Agar is a widely used medium, which supports the growth of wide variety of organisms even that of
fastidious ones such as Neisseria, Listeria and Brucella etc. The medium with addition of blood provides perfectly defined
haemolysis zones, while preventing the lysis of erythrocytes due to its sodium chloride content. It has been frequently used in
the health industry to produce antigens, toxins etc. It's simple and inhibitor-free composition makes it suitable for the detection
of antimicrobial agents in the food and other products. Tryptone Soya Agar is recommended by various pharmacopoeias as
sterility testing medium (6, 3).
Tryptone Soya Agar conforms as per USP (6) and is used in microbial limit test and antimicrobial preservative - effective test.
Gunn et al (2) used this medium for the growth of fastidious organisms and study of haemolytic reaction after addition of 5%
v/v blood. The combination of tryptone and soya peptone makes this media nutritious by providing amino acids and long
chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance..
Type of specimen
Isolated Microorganism
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the pack. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established
guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
2.Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the
medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Quality Control
Appearance
Sterile Soyabean Casein Digest Agar Plate w/ 5% Sheep blood in 90 mm disposable plates.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Colour of medium
Red coloured medium
Quantity of medium
25 ml of medium in 90 mm disposable plates.
pH
7.10-7.50
Sterility Test
Passes release criteria
Cultural response
Cultural characteristics was observed after an incubation for Bacterial at 30-35°C 18-24 hours and for Fungal at 30-35°C
<=5days.
Organism Inoculum Growth Recovery Haemolysis
(CFU)
Bacillus subtilis subsp. 50 -100 luxuriant >=70 % none
spizizenii ATCC 6633
(00003*)
Staphylococcus aureus 50 -100 luxuriant >=70 % beta
subsp. aureus ATCC
25923 (00034*)
Staphylococcus aureus 50 -100 luxuriant >=70 % beta
subsp. aureus ATCC
6538 (00032*)
Escherichia coli ATCC 50 -100 luxuriant >=70 % none
25922 (00013*)
Escherichia coli ATCC 8739 50 -100 luxuriant >=70 % none
(00012*)
Escherichia coli ATCC 50 -100 luxuriant >=70 % none
11775 (00090*)
Escherichia coli NCTC 50 -100 luxuriant >=70 % none
13167 (00179*)
Escherichia coli NCTC 9002 50 -100 luxuriant >=70 % none
Pseudomonas aeruginosa 50 -100 luxuriant >=70 % -
ATCC 27853 (00025*)
Pseudomonas aeruginosa 50 -100 luxuriant >=70 % -
ATCC 9027 (00026*)
Pseudomonas aeruginosa 50 -100 luxuriant >=70 % -
ATCC 10145 (00024*)
Salmonella Abony 50 -100 luxuriant >=70 % -
NCTC 6017 (00029*)
Micrococcus luteus ATCC 50 -100 luxuriant >=70 % -
9341
Streptococcus pneumoniae 50 -100 luxuriant >=70 % -
ATCC 6305
Salmonella Typhimurium 50 -100 luxuriant >=70 % -
ATCC 14028 (00031*)
Enterococcus faecalis 50 -100 luxuriant >=70 % -
ATCC 29212 (00087*)
Candida albicans ATCC 50 -100 luxuriant >=70 % -
10231 (00054*)
Candida albicans ATCC 50 -100 luxuriant >=70 % -
2091 (00055*)
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
# Aspergillus brasiliensis 50 -100 good 50-70% -
ATCC 16404 (00053*)
Clostridium perfringenes 50 -100 luxuriant >=70 % -
ATCC 13124 (00007*)
Key : (#)- Formerly known as Aspergillus niger (*) - Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label. Product performance is best if used within stated expiry
period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must
be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
Reference
1. Forbes B. A., Sahm A. S. and Weissfeld D. F., 1998, Bailey and Scotts Diagnostic Microbiology, 10th Ed., Mosby
Inc.St. Louis, Mo
2. Gunn B. A., Ohashi D K., Gaydos C. A., Holt E. S., 1977, J. Clin. Microbiol., 5(6) : 650.
3. Indian Pharmacopoeia, 2018, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India.
4. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
6. The United States Pharmacopoeia , 2019, The United States Pharmacopoeial Convention Inc., Rockville, MD.
Revision : 00/ 2019
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
[email protected] Website: www.himedialabs.com
