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Fehling Test

Fehling's test is used to detect the presence of reducing sugars. It involves mixing a sample with Fehling's solution A and B, which contain copper sulfate and an alkaline tartrate. Upon heating, a reducing sugar will cause the copper ions to reduce and form a reddish-brown copper (II) oxide precipitate. The test can differentiate between reducing sugars, which produce the precipitate, and non-reducing sugars or other substances, which do not. The document provides details on performing the test, including preparing the reagents and interpreting the results.

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2K views7 pages

Fehling Test

Fehling's test is used to detect the presence of reducing sugars. It involves mixing a sample with Fehling's solution A and B, which contain copper sulfate and an alkaline tartrate. Upon heating, a reducing sugar will cause the copper ions to reduce and form a reddish-brown copper (II) oxide precipitate. The test can differentiate between reducing sugars, which produce the precipitate, and non-reducing sugars or other substances, which do not. The document provides details on performing the test, including preparing the reagents and interpreting the results.

Uploaded by

Mg H
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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Fehling’s Test

Fehling’s Test

Objectives of Fehling’s Test


To detect the presence of carbohydrates in a solution.
To differentiate between reducing and non-reducing
sugars.

2
Fehling’s Test

Principle of Fehling’s test:


Fehling’s test is one of the sensitive test for detection of reducing sugars.
Fehling’s reagents comprises of two solution Fehling’s solution A and
solution B. Fehling’s solution A is aqueous copper sulphate and Fehling’s
solution B is alkaline sodium potassium tartarate ( Rochelle salt). Rochelle
salts (sodium potassium tartarate) present in the reagent acts as the chelating
agent in this reaction.These two solution are mixed in equal amount before
test.
On heating an aldehyde or reducing sugar with Fehling’s solution give
reddish brown prepitate. Formation of red precipitate of cuprous oxide
denotes the presence of reducing sugar.

2
Fehling’s Test

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Fehling’s Test

Requirements
1. Reagent
Fehling’s solution A: Dissolve 7 g of CuSO4.7H2O in 100 ml of water.
Fehlings solution B: Dissolve 24 g of KOH and 34.6 g of potassium sodium
tartrate in 100 ml water.
Fehling’s solution: Mix equal volumes of both the solution just before use.
Sample (5% Glucose, 5% Sucrose, 5% Fructose, 5% Starch, 5% lactose)
2. Materials Required
Pipettes
Test tubes
Test tube stand

2
Fehling’s Test

Pi Procedure of Fehling’s Test


Take 1 ml of a given sample in a clean, dry test tube. The concentration of
the test samples should be 5% (w/v).
Take control of 1 ml of distilled water in another tube.
Add about 2-3 drops of Fehling’s reagent to both the tubes and mix them in
a vortex.
Keep the test tubes in the water bath for 1-2 minutes.
Observe the appearance of color in the test tubes.
Note down the appearance of color seen in the test tubes.
pettes
Test tubes
Test tube stand
2
Fehling’s Test

•The appearance of a reddish-brown


precipitate indicates a positive
result and the presence of reducing
sugars.
•The absence of the reddish
precipitate or the appearance of
deep blue color indicates a negative
result and lack of reducing sugars.

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