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197 - Clone Contigs Mapping of Genomes

The document discusses the process of clone contigs mapping in genetics, which involves creating overlapping DNA fragments cloned into YAC/BAC vectors. It explains how these contigs are assembled into maps using techniques like FISH and DNA fingerprinting, and highlights challenges such as contamination from multiple chromosomal locations. The document emphasizes the transition from YAC to BAC vectors for improved sequencing projects.

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38 views14 pages

197 - Clone Contigs Mapping of Genomes

The document discusses the process of clone contigs mapping in genetics, which involves creating overlapping DNA fragments cloned into YAC/BAC vectors. It explains how these contigs are assembled into maps using techniques like FISH and DNA fingerprinting, and highlights challenges such as contamination from multiple chromosomal locations. The document emphasizes the transition from YAC to BAC vectors for improved sequencing projects.

Uploaded by

dc240200257zhu
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Genetics and Genomics

Clone Contigs
Mapping of
Genomes
Clone Contigs Mapping of Genomes

Clone Contigs Mapping


• A partial restriction
digest produces a set
of large, overlapping
DNAs, which are
cloned into YAC/BAC
vector
Clone Contigs Mapping of Genomes

Clone Contigs Mapping


• Shearing may also be
used to make high-
molecular-weight
DNA that is blunt-
end cloned into a
YAC/BAC.
Clone Contigs Mapping of Genomes

Clone Contigs Mapping


• An entire genome or
single chromosome
may be represented
in a YAC clone library
• YAC clones are then
assembled into a
map.
Clone Contigs Mapping of Genomes

Clone Contigs Mapping


• Maps can be
generated either by
matching with a
FISH-generated
chromosome map or
by DNA
fingerprinting
• Assembly of clone
contigs is based on
clone overlaps.
Clone Contigs Mapping of Genomes

Clone Contigs Mapping


• Non-polymorphic
short tandem
sequence are
especially useful for
YAC contig mapping
Clone Contigs Mapping of Genomes

Clone Contigs Mapping

• A complete library
should yield a
complete contig map
that indicates the
order in which the
cloned fragments
occur in the
chromosome
Clone Contigs Mapping of Genomes

Clone Contigs Mapping

• Problems arise when


some of the YAC
inserts contain DNA
from more than one
chromosomal
location.
• Complicated the
efforts at generating
a YAC contig map of
human chromosome
Clone Contigs Mapping of Genomes

Clone Contigs Mapping

• Many labs have


switched to BAC
vectors with a
capacity of 300 kb
and the ability to
replicate in E. coli as
a resource for their
sequencing projects.
Clone Contigs Mapping of Genomes

YAC Contigs Map of Chromosome 7


Clone Contigs Mapping of Genomes

Clone Contigs Mapping of Genomes


• A contig is a set of partially overlapping clones,
a contiguous set of clones. No gaps between
them.
• Contigs allow you to build up the sequence of
the chromosome over much larger regions than
any single clone.
• The first reasonably complete physical map of
the human genome involved contigs generated
by YACs.
Clone Contigs Mapping of Genomes

Clone Contigs Mapping of Genomes


Clone Contigs Mapping of Genomes

Clone Contigs Mapping of Genomes: Yac48-R


Clone Contigs Mapping of Genomes

Clone Contigs Mapping

• An entire genome or
single chromosome
may be represented
in a YAC/BAC clone
library

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