The development of antibody–drug conjugates remains a challenge in part due to the lack of three-dimensional structural information that must account for the inherent flexibility of antibodies and drug payloads. This Perspective discusses computational methods to guide the design of antibody–drug conjugates.

The discovery of lipoamide offers a unique approach to modulate stress granule dynamics. It will advance studies of stress granule biology and inform the therapeutic modification of these biomolecular condensates as a potential treatment option for amyotrophic lateral sclerosis.

We uncovered the function of a cell-bound biosurfactant, a glycine-glucolipid from Alcanivorax borkumensis that is synthesized via an unusual non-ribosomal peptide synthetase pathway. The glycine-glucolipid enhances microbial adhesion to oil droplets, enabling rapid degradation of the oil. Our research provides insights into advanced bioremediation strategies.

This Perspective discusses click biology as an analogy to click chemistry and examines reactions carried out using building blocks present in every living cell, enabling rapid selective covalent bond formation under biologically friendly conditions. Desirable criteria for robust cellular performance are defined, along with new opportunities arising from click biology for fundamental research and synthetic biology.

An ‘intracrine’ signaling mechanism is proposed whereby a G-protein-coupled receptor (free fatty acid receptor 4) senses locally released fatty acids on intracellular membranes associated with lipid droplets to efficiently regulate lipolysis in adipocytes.

Cryo-electron microscopy and biochemical reconstitution analysis reveals that Nde1 enhances Lis1 binding to autoinhibited dynein, promoting formation of a Phi-like–Lis1 intermediate during dynein activation.

LaccID, an engineered laccase, enables hydrogen-peroxide-free proximity labeling and electron microscopy (EM) in mammalian cells. Notably, LaccID is selectively active at the cell surface, enabling the mapping of the dynamic T cell–tumor surfaceome and its use as a genetically encodable EM tag, expanding the toolkit for cell-based imaging and proteomics.

Structural studies of certain Tn and STn antigen-targeting antibodies reveal that their V_H and V_L domains recognize the glycan antigen and the adjacent peptide region, respectively, enabling a V_H domain-focused and V_L domain-varying phage display library to generate antibodies targeting diverse glycopeptide epitopes.

A noncanonical pathway of DELLA regulation by a conserved inositol pyrophosphate synthase was identified in the liverwort, Marchantia polymorpha, that lacks the canonical gibberellin-induced DELLA degradation module found in angiosperms.

The primary entry route of vanilloid ligands to the vanilloid-binding site in transient receptor potential vanilloid 1 (TRPV1) is found to be a distinct and targetable hydrophobic pathway at the TRPV1–cell membrane interface rather than through direct membrane penetration.