Molecular biology articles from across Nature Portfolio

A matching pseudouridine in premature termination codons and in the anticodons of cognate tRNAs promotes ribosome readthrough and avoids nonsense-mediated mRNA decay.

Precise control of transgene expression remains a challenge in engineering primary cells for diverse applications. We developed DIAL, a promoter editing framework that transmits transient inputs into stable setpoints of expression in primary cells and human induced pluripotent stem cells, paving the way for predictable programming of gene circuits in therapeutically relevant cells.

In this study, we uncover the critical role of p300/CBP-mediated histone H2B N terminus multisite lysine acetylation (H2BNTac) in defining oncogenic enhanceosomes in prostate cancer. Degradation of p300/CBP rapidly disables H2BNTac-marked oncogenic enhancers and represents a promising therapeutic strategy for enhancer-driven malignancies, including prostate cancer.

Goel et al. produce high-resolution three-dimensional genome structure mapping from mitosis to G1 phase to show unseen interactions between enhancers and promoters in prometaphase. Polymer modeling indicates the interactions are facilitated by chromosome compaction.

Spider mites produce adhesive silk fibers for web spinning. This study reveals that proteins previously thought to be silk components are salivary proteins with dual roles in feeding and silk fiber coating.

In this Tools of the Trade article, Okuda and Kessler (Müller-McNicoll and Heilemann labs) discuss how the combination of two novel methods enabled them to study the architecture and interaction of nuclear membraneless organelles.

A matching pseudouridine in premature termination codons and in the anticodons of cognate tRNAs promotes ribosome readthrough and avoids nonsense-mediated mRNA decay.

Precise control of transgene expression remains a challenge in engineering primary cells for diverse applications. We developed DIAL, a promoter editing framework that transmits transient inputs into stable setpoints of expression in primary cells and human induced pluripotent stem cells, paving the way for predictable programming of gene circuits in therapeutically relevant cells.

In this study, we uncover the critical role of p300/CBP-mediated histone H2B N terminus multisite lysine acetylation (H2BNTac) in defining oncogenic enhanceosomes in prostate cancer. Degradation of p300/CBP rapidly disables H2BNTac-marked oncogenic enhancers and represents a promising therapeutic strategy for enhancer-driven malignancies, including prostate cancer.

Massively parallel reporter assays (MRPAs) are used in vascular smooth muscle cells to measure the functional effects of over 25,000 variants associated with coronary artery disease. This approach identifies regulatory variants in moderate linkage to disease-associated loci, implicating a broader spectrum of causal variants.