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Process Illumina instrument data into SAM/BAM/CRAM files.

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bambi

A set of programs to manipulate SAM/BAM/CRAM files, using HTSLIB

configuration

to re-enable converting warnings to errors, un-comment HTS_PROG_CC_WERROR(bambi_late_cflags) in configure.ac

installation

autoreconf -i

configure --prefix=<place to install> --with-htslib=<directory containing lib and include directory with htslib release>

eg: configure --prefix=/usr/local/bambi --with-htslib=/usr/local/htslib/1.3

or: configure --prefix=$HOME/programs --with-htslib=$CONDA_PREFIX

if conda is activated and contains htslib, pkg-config plus compilers simply: configure --prefix=$HOME/programs

make or make -j <threads>

make check (to run tests)

make install

usage

bambi <command> [options]

command description
decode decode a multiplexed SAM/BAM/CRAM file by read groups
i2b converts illumina files to SAM/BAM/CRAM files
select select reads by alignment
chrsplit split reads by chromosome
read2tags convert reads into tags
spatial_filter spatial filtering
seqchksum calculate checksums for a bam file
adapters find and remove adapters
update update a BAM/SAM/CRAM file by RG tag
tag2qname prefix qnames of a BAM/SAM/CRAM file by tag value
substitution_analysis produce a substitution analysis table

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Process Illumina instrument data into SAM/BAM/CRAM files.

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