Rhizopus stolonifer LAU 07: a novel source of fructosyltransferase
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Agbaje Lateef
, Julius Oloke
Abstract
Increasing awareness of the importance of fructooligosaccharides (FOS) as ingredients of functional foods has led to intensive search of new sources of fructosyltransferases (FTase), enzymes responsible for the conversion of sucrose to fructooligosaccharides. A local strain of Rhizopus stolonifer isolated from spoilt orange fruit with high fructosyltransferase activity (U t) of 12.31–45.70 U mL−1 during a fermentation period of 24–120 h is herein reported. It showed low hydrolytic activity (U h) in the range of 0.86–1.78 U mL−1 during the same period. FOS yield of 34 % (1-kestose, GF2, nystose, GF3) was produced by FTase obtained from a 72 h-old culture using 60 g of sucrose per 100 mL of the substrate. When the isolate was grown in a defined submerged medium, its pH dropped sharply from the intial value of 5.5 to 1.0 within 24 h, and this value was maintained throughout the fermentation. The biomass content ranged from 8.8 g L−1 at 24 h of fermentation to reach the maximum of 10 g L−1 at 72 h. It was reduced to 5.6 g L−1 at the end of 120 h of fermentation. This report represents the first reference to a strain of Rhizopus as a source of FTase for the production of FOS. The high U t/U h ratio shown by this isolate indicates that it may be a good strain for the industrial and commercial production of FOS. However, there is a need of further optimization of the bioprocess to increase the conversion efficiency of sucrose to FOS by the enzyme.
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© 2008 Institute of Chemistry, Slovak Academy of Sciences
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Articles in the same Issue
- Investigation of 3-amino-1,2,4-triazole azodye derivatives as reagents for determination of mercury(II)
- Electrospray ionization mass spectra of pentoses, hexoses, and 2-deoxy-2-fluoro-d-glucose
- Influence of solution composition and iron powder characteristics on reduction of 2,4,6-trinitrophenol
- Electrochemical behaviour of lanthanum fluoride in molten fluorides
- Structural and electronic effects involving pyridine rings in 4-methylpyridine Cu4OX6L4 complexes. II. Correlations based on molecular structure of the Cu4OCl6(4-Mepy)4 complex
- Photoswitching of a heptanuclear FeII-FeIII complex — A case of multifunctional magnetic materials
- Collagen-grafted ultra-high molecular weight polyethylene for biomedical applications
- Synthesis of macrocyclic polyethers and polyether diesters and preparation of their cationic complexes
- Prediction of anti-HIV activity and cytotoxicity of pyrimidinyl and triazinyl amines: A QSAR study
- Composition of the essential oil of Geocaryum cynapioides (Guss.) L. Engstrand
- Photolytic degradation of triclosan in the presence of surfactants
- Determination of dissociation degrees of K3NbF8 and K3TaF8 by thermodynamic analysis of subsystems of the KF-K2NbF7 and KF-K2TaF7 systems
- DFT-based quantum theory QSPR studies of molar heat capacity and molar polarization of vinyl polymers
- Silica sulfuric acid-catalyzed expeditious environment-friendly hydrolysis of carboxylic acid esters under microwave irradiation
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