This is the repository for the study published in this paper. This paper presents a self-supervised model for classifying white blood cells in peripheral blood smears, achieving high accuracy (F1: 96.2%) while generalizing to diverse label sets. The lightweight EfficientNetV2-B0-based approach enhances label efficiency with active learning and is available as the HemoSight web app to streamline clinical workflows.

General flow of data pipeline:

1. curate.py: curate raw data folders to create data reference csv.
2. loader.py: Load data reference csv and perform train, validation, and test split.
3. train.py, generator.py: Train model; data augmentation generated from generator.
4. val.py, predictor.py: Validate trained model; predictor performs classification on embeddings.

Folders:

• src stores python source code.
• frontend stores frontend source code.
• data is used to store raw data.
• derived is used to store results.
• mongodb is used by the MongoDB database. Three folders (data, derived, src) are needed for file I/O, and should be mounted to the container.

Configuration files:

• Path configuration file (/src/core/settings.json):
  This file is loaded by util.GlobalSettings. settings.json is used by default but can be overwritten by settings_{systemname}.json where {systemname} is the computer name such as ThinkPad. This enables the same repository code base to be cloned and run on multiple environments.
• Job configuration file: Some examples are located at /src/config_*.json. See below for their usage.

1. Create environment conda create -n HemoSight python=3.10 and initialize the environment.
2. Install packages listed in the dockerfile.
3. You may also need to install ipykernel for editing ipynb files.

Dockerfile.gpu is the GPU version. Dockerfile.cpu is the CPU version.

1. Build docker build -t hematology:v1 -f Dockerfile.cpu .
2. Run the container with above three folder mounted docker run -it --gpus all --rm -v "$(pwd)/src:/src" -v "$(pwd)/derived:/derived" -v "D:/Drive/Data/Hematology:/data" hematology:v1
3. After the container is running, execute the following in the container.
   • Training python -m model.train --cfg config.json
   • Validation python -m model.val --run 20231208192208

You may need to install node.js 20.11, MongoDB 7.0.

1. Build docker-compose -f docker-compose.dev.yaml up --build
2. Access http://localhost:4002 for index page.

If you find this repository helpful in your research or work, please consider citing our paper:

@inproceedings{liu2024adaptive,
  title={Adaptive self-supervised learning of morphological landscape for leukocytes classification in peripheral blood smears},
  author={Liu, Z., Castillo, S. P., Han, X., Sun, X., Hu, Z., and Yuan, Y.},
  booktitle={Proceedings of the IEEE-EMBS International Conference on Biomedical and Health Informatics},
  year={2024},
  url={https://openreview.net/forum?id=xkgKn92AGp}
}

Feel free to contact us for further information or questions related to the paper and this repository.

Yuan Lab @ MD Anderson Cancer Center