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Scripts analysisng the Hungate1000 Culture Collection

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The Hungate 1000 prokaryotic culture collection encodes a wide variety of bacteriocinss

This project mines 410 rumen-derived genomes from the Hungate1000 collection to explore bacteriocin biosynthetic potential. We identify 408 novel bacteriocin gene clusters across 308 genomes, including multiple previously undescribed bacteriocins and producer species.

All 410 cultivated reference genomes are available from the Joint Genome Institute with under the DOI 10.46936/10.25585/60000534 or from NCBI under the Umbrella project accession PRJNA471733. Here are the scripts analysing the Hungate1000 Culture Collection for bacteriocin gene clusters.

Interactive GMSC Table

Click here to explore the interactive HTML table

Interactive GenBank Table

Click here to explore the interactive HTML table

This study aims to identify bacteriocins encoded by ruminant-associated bacteria to establish a foundation for microbiome-native antimicrobial strategies capable of modulating rumen community structure and function. A secondary aim is to explore the ecological and evolutionary context of these bacteriocins, including their association with host specificity and microbial competition within and outside the rumen ecosystem. Hungate 1000 rationale

The bacteriocins encoded in the Hungate 1000 culture collection are found in the global microbiome when looking at exact core peptides.

The bacteriocins encoded in the Hungate 1000 culture collection are found in ruminant animals.

Conclusion

Overall, ~30% of rumen isolates encode known antimicrobial bacteriocins, rising to ~70% when including less-characterised peptide classes, highlighting the rumen as a rich reservoir of antimicrobial diversity.

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DEHourigan

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For any queries, please reach out via GitHub issues or directly to [email protected] or [email protected].

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