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FABind: Fast and Accurate Protein-Ligand Binding (NeurIPS 2023)

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FABind: Fast and Accurate Protein-Ligand Binding 🔥

Overview

This repository contains the source code for Neurips 2023 paper "FABind: Fast and Accurate Protein-Ligand Binding". FABind achieves accurate docking performance with high speed compared to recent baselines. If you have questions, don't hesitate to open an issue or ask me via [email protected], Kaiyuan Gao [email protected], or Lijun Wu via [email protected]. We are happy to hear from you!

News

Oct 10 2023: The trained FABind model and processed dataset are released!

Oct 11 2023: Initial commits. More codes, pre-trained model, and data are coming soon.

Setup Environment

This is an example for how to set up a working conda environment to run the code. In this example, we have cuda version==11.3, and we install torch==1.12.0. To make sure the pyg packages are installed correctely, we directly install them from whl.

conda create --name fabind python=3.8
conda activate fabind
conda install pytorch==1.12.0 torchvision==0.13.0 torchaudio==0.12.0 cudatoolkit=11.3 -c pytorch
pip install https://data.pyg.org/whl/torch-1.12.0%2Bcu113/torch_cluster-1.6.0%2Bpt112cu113-cp38-cp38-linux_x86_64.whl
pip install https://data.pyg.org/whl/torch-1.12.0%2Bcu113/torch_scatter-2.1.0%2Bpt112cu113-cp38-cp38-linux_x86_64.whl
pip install https://data.pyg.org/whl/torch-1.12.0%2Bcu113/torch_sparse-0.6.15%2Bpt112cu113-cp38-cp38-linux_x86_64.whl 
pip install https://data.pyg.org/whl/torch-1.12.0%2Bcu113/torch_spline_conv-1.2.1%2Bpt112cu113-cp38-cp38-linux_x86_64.whl
pip install https://data.pyg.org/whl/torch-1.12.0%2Bcu113/pyg_lib-0.2.0%2Bpt112cu113-cp38-cp38-linux_x86_64.whl
pip install torch-geometric
pip install torchdrug==0.1.2 rdkit torchmetrics==0.10.2 tqdm mlcrate pyarrow accelerate Bio lmdb fair-esm tensorboard
pip install fair-esm

Data

The PDBbind 2020 dataset can be download from http://www.pdbbind.org.cn. We then follow the same data processing as TankBind.

We also provided processed dataset on zenodo. If you want to train FABind from scratch, or reproduce the FABind results, you can:

  1. download dataset from zenodo
  2. unzip the zip file and place it into data_path such that data_path=pdbbind2020

Generate the ESM2 embeddings for the proteins

Before training or evaluation, you need to first generate the ESM2 embeddings for the proteins based on the preprocessed data above.

data_path=pdbbind2020

python fabind/tools/generate_esm2_t33.py ${data_path}

Then the ESM2 embedings will be saved at ${data_path}/esm2_t33_650M_UR50D.lmdb.

Model

The pre-trained model is placed at ckpt/best_model.bin.

Evaluation

data_path=pdbbind2020
ckpt=ckpt/best_model.bin

python fabind/test_fabind.py \
    --batch_size 4 \
    --data-path $data_path \
    --resultFolder ./results \
    --exp-name test_exp \
    --ckpt $ckpt_path \
    --local-eval

Inference on Custom Complexes

Coming soon...

Re-training

data_path=pdbbind_2020
# write the default accelerate settings
python -c "from accelerate.utils import write_basic_config; write_basic_config(mixed_precision='no')"
# "accelerate launch" will run the experiments in multi-gpu if applicable 
accelerate launch fabind/main_fabind.py \
    --batch_size 12 \
    -d 0 \
    -m 5 \
    --data-path $data_path \
    --label baseline \
    --addNoise 5 \
    --resultFolder ./results \
    --use-compound-com-cls \
    --total-epochs 500 \
    --exp-name train_tmp \
    --coord-loss-weight 1.0 \
    --pair-distance-loss-weight 1.0 \
    --pair-distance-distill-loss-weight 1.0 \
    --pocket-cls-loss-weight 1.0 \
    --pocket-distance-loss-weight 0.05 \
    --lr 5e-05 --lr-scheduler poly_decay \
    --distmap-pred mlp \
    --hidden-size 512 --pocket-pred-hidden-size 128 \
    --n-iter 8 --mean-layers 4 \
    --refine refine_coord \
    --coordinate-scale 5 \
    --geometry-reg-step-size 0.001 \
    --rm-layernorm --add-attn-pair-bias --explicit-pair-embed --add-cross-attn-layer \
    --noise-for-predicted-pocket 0 \
    --clip-grad \
    --random-n-iter \
    --pocket-idx-no-noise \
    --pocket-cls-loss-func bce \
    --use-esm2-feat

About

Citations

@article{pei2023fabind,
  title={FABind: Fast and Accurate Protein-Ligand Binding},
  author={Pei, Qizhi and Gao, Kaiyuan and Wu, Lijun and Zhu, Jinhua and Xia, Yingce and Xie, Shufang and Qin, Tao and He, Kun and Liu, Tie-Yan and Yan, Rui},
  journal={arXiv preprint arXiv:2310.06763},
  year={2023}
}

Acknowledegments

We appreciate EquiBind, TankBind, E3Bind, DiffDock and other related works for their open-sourced contributions.

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FABind: Fast and Accurate Protein-Ligand Binding (NeurIPS 2023)

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