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Spatial Transcriptomics Delineate Dedifferentiation and Molecular Characterizations in Lung Adenocarcinoma Progression

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ST_LUAD

Spatial Transcriptomics Delineate Dedifferentiation and Molecular Characterizations in Lung Adenocarcinoma Progression

Features

  • Dimensionality Reduction, clustering, FindMarkers
  • Cell type mapping and deconvolution
  • Spatial correlation analysis
  • Multimodal intersection analysis (MIA)
  • Dedifferentiation trajectory inference
  • Gene Ontology Enrichment Analysis
  • Jaccard index
  • Spatial ligand-receptor interaction analysis
  • mIHC image quantifications

Softwares

  • Seurat (V3.2)
  • Monocle (V2.18.0)
  • CytoTRACE (V0.3.3)
  • clusterProfiler (V3.16.1)
  • SCENIC (V 1.2.2)
  • GSVA (V1.32.0)
  • MSigDB (V7.3)
  • Cytoscape (V3.6.0)
  • CellPhoneDB (V2.1.4)
  • inForm (V2.4)
  • QuPath (V0.2.0)
  • survival (V3.2-10)
  • survminer (V0.4.6)
  • R package: stats (V3.6.0), FSA (V0.9.1), e1071 (V1.7-3), pheatmap (V1.0.12), psych (V1.9.12.31)

Prerequisites

  • Linux x86_64 GNU/Linux or UNIX
  • R >= 3.6.0
  • perl >= v5.16.3

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