עמרי נהור 211359054 ניצן רפאל מגדל 206763351 יעל בורוכוב 323029637

Bioinformatics Final Project This project analyzes a GenBank file for Bacillus clausii and compares it to UniProt data.

Files main.py - Main script to analyze GenBank file part_b/part_b.py - Script to analyze UniProt data part_c/part_c.py - Script to retrieve sequences from NCBI using accession numbers Usage The main scripts can be run as:

part a: python main.py part b: python part_b/part_b.py part c: python part_c/one.py python part_c/two.py python part_c/dnds.py

main.py will output statistics and plots based on analyzing the GenBank file.

part_b.py will analyze the UniProt data and output hydrophobicity statistics.

part_c is divided into 3 parts: one.py - prints according to the standard table all the codons and their options to be synonymous and non-synonymous two.py-checks whether there are shared genes and prints them (there are no different genes between the 2 files) dnds.py-performs dnds calculation and prints 5 common genes. 2 attachments: April.gb February.gb that we downloaded about the corona virus.

Results Key results from the analysis include:

Statistics on gene features in GenBank file Comparison of genes on positive vs negative strands Histogram of gene lengths Analysis of TC count for each gene Comparison of GenBank vs UniProt gene names Hydrophobicity analysis of UniProt proteins Contributing Contributions to expand the analysis are welcome!

License This project is unlicensed.