February 21, 2025

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09/04/2025

research article

Spatial colocalization and molecular crosstalk of myofibroblastic CAFs and tumor cells shape lymph node metastasis in oral squamous cell carcinoma

In this study, the authors integrated bulk genome and transcriptome, single-cell transcriptome, and spatial transcriptome data from OSCC and found that myCAFs were quantitatively and functionally activated in LNM-positive samples and spatially colocalized with OSCC cells at the invasive tumor front, providing a niche that may facilitate LNM.

Image credit: pgen.1011791

Spatial colocalization and molecular crosstalk of myofibroblastic CAFs and tumor cells shape lymph node metastasis in oral squamous cell carcinoma

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Current Issue September 2025

08/26/2025

formal comment

On the role of the terminal oxidase cytochrome bd in hyper-resistance of Listeria monocytogenes to the macrodiolide antibiotic tartrolon B

This commentary discusses how the terminal oxidase cytochrome bd contributes to resistance of Listeria monocytogenes against the antibiotic tartrolon B. The authors outline evidence that cytochrome bd, but not cytochrome aa3, is required for resistance, and propose alternative models for how this oxidase helps maintain proton motive force and protect against the compound.

Image credit: pgen.1011803

On the role of the terminal oxidase cytochrome bd in hyper-resistance of Listeria monocytogenes to the macrodiolide antibiotic tartrolon B

08/14/2025

methods

Defining transcription factor nucleosome binding with Pioneer-seq

This study introduces Pioneer-seq, a method to examine transcription factor binding across thousands of nucleosomes. Using OCT4, SOX2, KLF4, and MYC, the authors show that all can bind at nucleosome edges and that nucleosome sequence is the primary factor regulating binding. KLF4 and SOX2 also bind near nucleosome centers, helping define the mechanistic requirements for pioneer factor binding.

Image credit: pgen.1011813

Defining transcription factor nucleosome binding with Pioneer-seq

09/05/2025

research article

Cell splitting in Staphylococcus aureus is controlled by an adaptor protein facilitating degradation of a peptidoglycan hydrolase

This study identifies CxaR as an adaptor protein that enables ClpXP-mediated degradation of the peptidoglycan hydrolase Sle1, revealing a new regulatory factor controlling Staphylococcus aureus cell splitting and offering insight into β-lactam antibiotic action.

Image credit: pgen.1011841

09/02/2025

research article

Conservation and divergence of ciprofloxacin persister survival mechanisms between Pseudomonas aeruginosa and Escherichia coli

This study shows that while homologous recombination and the SOS response promote ciprofloxacin persistence in both E. coli and P. aeruginosa, their contributions differ by growth phase, highlighting both conservation and divergence of persister survival mechanisms.

Image credit: pgen.1011840

09/02/2025

research article

Deficiency of Kif15 impairing synaptic development leads to mood disorder in mice

The authors show that Kif15 deficiency disrupts dendritic morphology and synaptic function in mice, contributing to mood disorder-like behaviors. Kif15-/- mice had reduced postsynaptic proteins and dendritic spines in adolescence, abnormal increases in adulthood, and overexpression of Kif15 rescued depressive behavior in zebrafish.

Image credit: pgen.1011839

09/05/2025

research article

Charting the equine miRNA landscape: An integrated pipeline and browser for annotating, quantifying, and visualizing expression

The authors developed FARmiR and AIMEE to profile miRNAs and isomiRs, generating the most comprehensive equine miRNA atlas from 461 RNA-seq datasets.

Image credit: pgen.1011835

08/29/2025

research article

Igf2 adult-specific skeletal muscle enhancer activity revealed in mice with intergenic CTCF boundary deletion

Here, the authors investigate the CTCF-bound centrally conserved domain (CCD), intergenic to H19 and Igf2, and an adjacent widely expressed lncRNA.

Image credit: pgen.1011834

08/25/2025

research article

Tissue-specific consequences of tag fusions on protein expression in transgenic mice

Using a series of genome edited mouse models, the authors show that tag-dependent changes in protein expression can vary across different primary cell and tissue contexts.

Image credit: pgen.1011830

08/22/2025

research article

Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter

The authors developed a riboswitch-luciferase reporter to track (p)ppGpp in Bacillus subtilis, enabling dynamic in vivo measurement and linking its metabolism to gene regulation.

Image credit: pgen.1011691

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PLOS Reviewer Center

A collection of free training and resources for peer reviewers of PLOS journals—and for the peer review community more broadly—drawn from research and interviews with staff editors, editorial board members, and experienced reviewers.
Submit your Lab and Study Protocols to PLOS ONE!

PLOS ONE is now accepting submissions of Lab Protocols, a peer-reviewed article collaboration with protocols.io, and Study Protocols, an article that credits the work done prior to producing and publishing results.
Integrating OpenAlex metadata to improve Open Science Indicators

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What's happening at PLOS Genetics?

Discover Our Updated Editorial Board Webpage!

We're excited about our revised and synchronized Editorial Board webpage—now enhanced with a brand-new search functionality!

Easily find and explore board members with our improved layout and seamless navigation.
PLOS Genetics welcomes new Section Editors in 2024

We've been thrilled to welcome:

Marnie Blewitt, Giovanni Bosco, Gregory M. Cooper, Paula Cohen, Monica Colaiacovo, Danielle Garsin, Kent Hunter, Hongbin Ji, Zoltan Kutalik, Anne O'Donnell-Luria, Giorgio Sirugo, Pablo Wappner, and Fengwei Yu!
Harmit Malik receives Novitski Prize

Congratulations to Associate Editor Harmit Malik, who was awarded the Edward Novitski Prize by the Genetics Society of America for his work on genetic conflict. Harmit has also been elected as a new member of the American Academy of Arts & Sciences.

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