Abstract
Objective
Extracellular fructosyltransferase (FTase, E.C.2.4.1.9) from Aspergillus oryzae IPT-301 was immobilized on silica gel by adsorption and biochemically characterized aiming at its application in the transfructosylation reaction of sucrose for the production of fructooligossaccarides (FOS).
Results
The transfructosylation activity (AT) was maximized by the experimental design in function of the reaction pHs and temperatures. The AT of the immobilized enzyme showed the kinetics behavior described by the Hill model. The immobilized FTase showed reuse capacity for six consecutive reaction cycles and higher pH and thermal stability than the soluble enzyme.
Conclusion
These results suggest a high potential of application of silica gel as support for FTase immobilization aiming at FOS production.
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Acknowledgements
The authors gratefully acknowledge the financial support from the National Council for Scientific and Technological Development—CNPq (Proc. 421540/2018-4), Foundation for Research of the State of Minas Gerais (FAPEMIG, Proc. APQ-02131-14) and Coordination for the Improvement of Higher Education Personnel (CAPES).
Supporting Information—Supplementary Figures
Figure S1 Linear adjustment for the determination of the thermal denaturation constant (kD) of the soluble (A) and immobilized FTase on in silica gel (B).
Figure S2 Determination of activation energy for the thermal denaturation (ED). Arrhenius plot of ln (kD) versus (1/T) was used for ED of soluble (■) and immobilized (□) extracellular FTase from Aspergillus oryzae IPT-301.
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Faria, L.L., Morales, S.A.V., Prado, J.P.Z. et al. Biochemical characterization of extracellular fructosyltransferase from Aspergillus oryzae IPT-301 immobilized on silica gel for the production of fructooligosaccharides. Biotechnol Lett 43, 43–59 (2021). https://doi.org/10.1007/s10529-020-03016-7
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DOI: https://doi.org/10.1007/s10529-020-03016-7