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Molecular profile of African swine fever virus (ASFV) circulating in Vietnam during 2019-2020 outbreaks

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Abstract

African swine fever (ASF) is a highly infectious disease of pigs caused by African swine fever virus (ASFV). In order to identify potential genetic variations among ASFV strains circulating in Vietnam, 26 ASFV isolates from organs and blood samples collected from domestic pigs from 23 different provinces of northern, central and southern Vietnam during 2019-2020 ASF outbreaks were genetically characterized. Nucleotide sequences were determined for a portion of the B646L (p72) gene, the complete E183L (p54) gene, the variable region of EP402R (CD2v), the central variable region (CVR) of pB602L, and a tandem repeat sequence (TRS) between the I73R and I329L genes. Analysis of the partial B646L (p72) and EP402R (CD2v) gene sequences and the full-length E183L (p54) gene sequence showed that all 26 ASFV isolates belonged to genotype II and serotype VIII and that they were identical to the strain Georgia/2007/1 and all ASFV strains sequenced in China. The TRS between the I73R and I329L genes contained a 10-nucleotide insertion that was observed in the Chinese ASFV strain CN201801 isolated from domestic pigs in 2018, but not in the Georgia/2007/1 and China/Jilin/2018/boar strains isolated from wild boar in China. This is the first intra-epidemic genome analysis reported for the ASFV strains circulating in Vietnam.

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Funding

This work was supported by the Vietnam National Project under project code no. DTDL.CN-53/19 and by a Grant from the Animal and Plant Quarantine Agency (Grant no. I-1543085-2019-21-01), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.

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Author notes

  1. Nguyen Tuan Anh Mai, Xuan Dang Vu, and Thi Thu Huyen Nguyen contributed equally to this article.

Authors and Affiliations

  1. College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam

    Nguyen Tuan Anh Mai, Xuan Dang Vu, Thi Thu Huyen Nguyen, Van Tam Nguyen, Thi Bich Ngoc Trinh, Thi Lan Nguyen, Thi To Nga Bui & Van Phan Le

  2. Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon, South Korea

    Yong Joo Kim, Hyun-Joo Kim & Ki-Hyun Cho

  3. Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea

    Dae Gwin Jeong & Sun-Woo Yoon

  4. National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada

    Thang Truong & Aruna Ambagala

  5. College of Pharmacy, Korea University, Sejong, South Korea

    Daesub Song

Authors
  1. Nguyen Tuan Anh Mai
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  2. Xuan Dang Vu
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Correspondence to Van Phan Le.

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Mai, N.T.A., Vu, X.D., Nguyen, T.T.H. et al. Molecular profile of African swine fever virus (ASFV) circulating in Vietnam during 2019-2020 outbreaks. Arch Virol 166, 885–890 (2021). https://doi.org/10.1007/s00705-020-04936-5

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  • DOI: https://doi.org/10.1007/s00705-020-04936-5