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VAPER: Viral Assembly from Probe-based EnRichment

Key Features

VAPER is a viral (meta-)assembly pipeline that can:

🧬 Assemble genomes from complex samples, supporting multiple assemblies per sample (e.g., co-infections)
🧬 Automatically detect and select reference genomes
🧬 Predict the taxonomy of each assembly, with an optional viral metagenomic summary
🧬 Export reads associated with each assembly for downstream use

While VAPER was originally designed for hybrid capture data (e.g., Illumina VSP or Twist CVRP), it has also been used with shotgun metagenomic and tile-amplicon data. It comes stock with comprehensive reference sets for multiple taxa, including influenza A, SARS-CoV-2, and Monkeypox (full list). Keep on reading to learn more!

Pipeline Overview

VAPER Flowchart

More Information:

See the docs for more information.

Acknowledgements

VAPER would not be possible without the following people:

  • Holly Halstead (WA PHL)
  • Pauline Trinh (WA MEP)
  • Allison Black (WA MEP)
  • Stephanie Lunn (WA MEP)
  • Kristen Waterman (WA PHL)
  • Brandi Torrevillas (WSU)

VAPER was originally written by Jared Johnson for the Washington State Department of Health.