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RSeQC - Normalise junction saturation plot#3216

Merged
vladsavelyev merged 3 commits into
MultiQC:mainfrom
pontushojer:rseqc-junc-sat
Jun 6, 2025
Merged

RSeQC - Normalise junction saturation plot#3216
vladsavelyev merged 3 commits into
MultiQC:mainfrom
pontushojer:rseqc-junc-sat

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@pontushojer

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The junction saturation counts become hard to overview for multiple samples if they vary greatly in the number of junctions. For example, see the plot below generated using the current MultiQC.

rseqc_junction_saturation_plot

The sample with the highest counts obscures all other samples. You could zoom in to observe the other samples, but it is not too practical to evaluate this metric quickly for a large number of samples.

To make this plot more useful, this PR normalises the junction counts to the total number of junctions, which brings all samples into the same scale to make the plot easier to evaluate

rseqc_junction_saturation_plot (1)

PS. I also removed a hint that is no longer relevant.

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@pontushojer pontushojer changed the title RseQC - Normalize junction saturation plot RSeQC - Normalise junction saturation plot Jun 4, 2025

@vladsavelyev vladsavelyev left a comment

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Looks good, thank you! 😌

@vladsavelyev vladsavelyev added this to the v1.30 milestone Jun 6, 2025
@vladsavelyev vladsavelyev merged commit dcf93b8 into MultiQC:main Jun 6, 2025
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@pontushojer pontushojer deleted the rseqc-junc-sat branch December 8, 2025 15:35
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2 participants