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Lab Report Integrated Science

1) The experiment aimed to calculate the concentration of an unknown solution using a calibration curve relating the absorbance and concentration of copper sulfate (CUSO4) solutions. 2) Materials and procedures included preparing known CUSO4 solutions, measuring their absorbance spectrophotometrically, and plotting a calibration curve of absorbance versus concentration. 3) Analysis of the calibration curve, which had an R2 value of 0.999, allowed calculation of the concentration of an unknown as 0.033M.

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174 views3 pages

Lab Report Integrated Science

1) The experiment aimed to calculate the concentration of an unknown solution using a calibration curve relating the absorbance and concentration of copper sulfate (CUSO4) solutions. 2) Materials and procedures included preparing known CUSO4 solutions, measuring their absorbance spectrophotometrically, and plotting a calibration curve of absorbance versus concentration. 3) Analysis of the calibration curve, which had an R2 value of 0.999, allowed calculation of the concentration of an unknown as 0.033M.

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LAB REPORT INTEGRATED SCIENCE

Introduction
In this experiment, the calculated mass and actual mass was used to calculate the
concentration of a particular chemical substance. By this, the knowledge taught in class was
used to investigate the concentration of an unknown solution by using the calibration curve.
Also, this knowledge could be developed and used in future study: forensic science.

Materials

Procedure
1. Wear labcoat, tie your hair
2. Put on gloves
3. Prepare materials : spatula, massing paper, balance spectrophotometer CUSO4
(copper sulfate) and H2O (water) , volumetric flask
4. Place the massing paper on the balance and press tear
5. Use the spatula to put CUSO4 on to the balance and weight them (after weighing all
of the 4 masses we need)
6. Dissolve CUSO4 in a beaker then transfer the substance into volumetric flask/ adjust
volume to 100.00ml
7. Dissolce the CUSO4 and H2O in the spectophometer
8. Dissolve the response - absorbance at 810nm (3 trails)
9. Place the data into excel sheet
10. Create the graph in excel with standard calibration curve
Data
The equation that we use to calculate for the concentration is
T heorical concentration(M )
249.68 g/mol(molecular weight)0.1L
For the absorbance, we averaged the absorbance of 3 trials
T rial 1 result + T rial 2 result + T rial 3 result
3
Report the value of slope, y-intercept, and R2
=slope(array of y- absorbance, array of x- concentration)

=intercept(array of y- absorbance, array of x- concentration)

=rsq(array of y- absorbance, array of x- concentration)


Results and Discussion
The relation of concentration and absorbance is directly proportional to one another as the
graph shows a straight line. It can be clearly seen as stated in the equation
y= 12.015x-0.111. Our R2 is also quite satisfying because ours was precise with
0.999659402 and in order for it to be accurate it has to be equal to 1. Our standard
calibration curve is also good because since the R2 is quite accurate, it means that our dots
that are plotted are close to the standard curve. In addition, the linearity of the line is quite
straight, meaning that it is precise. Moreover, Y intercept is the Y value when X value is
equal to zero. The best value of the Y-intercept is the point (0,0).

Find unknown out of concentration


y= 12.015x-0.0111
y= 0.386A
0.386 = 12.015x-0.0111
0.386+0.0111= 12.015x
0.3971= 12.015x
0.3971
x= 12.015
x=0.03305035(M)

However, the absorbance of a greater concentration should be tested in order to make good
prediction of a higher amount of concentration.

Conclusion
Overall, the relation of the concentration and absorbance is directly proportional. Since our
standard calibration curve is quite accurate, the equation that was discovered in this
experiment could be used to make future assumption of the absorbance produced after
increasing the concentration of the solution.

Error
The distilled water mixing with copper sulfate has been kept in the beaker for too long, this
might affect the accuracy of the numbers according to the fading intensity of the substance.
Moreover, another error is that the distilled water has been pour over a line marked on a
cuvette, the accurateness of the light passing through the cuvette will be decreased which
will produce inaccurate numbers.

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